domestic dog origin of canine distemper virus in free-ranging wolves in portugal as revealed by hemagglutinin gene characterization.serologic evidence for canine distemper virus (cdv) has been described in grey wolves but, to our knowledge, virus strains circulating in wolves have not been characterized genetically. the emergence of cdv in several non-dog hosts has been associated with amino acid substitutions at sites 530 and 549 of the hemagglutinin (h) protein. we sequenced the h gene of wild-type canine distemper virus obtained from two free-ranging iberian wolves (canis lupus signatus) and from one domestic dog (canis f ...201121719841
echinococcus granulosus (cestoda, taeniidae) in the iberian wolf.the intestinal contents of 27 iberian wolves (canis lupus signatus) from spain were screened for the presence of the taenid cestode echinococcus granulosus. four animals were found positive (15% prevalence). the intensity of parasitation was variable (mean 71, range 1-147 e. granulosus per host). gravid individuals were found in all wolves positive with the parasite. molecular characterization of the parasite material showed that the wolf strain belongs to the g1 genotype. according to the resul ...200616752157
new techniques for an old disease: sarcoptic mange in the iberian wolf.sarcoptic mange, a parasitic skin infection caused by the burrowing mite sarcoptes scabiei, has been reported in over 100 mammals, including humans. in endangered species, mange causes conservation concerns because it may decimate isolated populations and contribute to extinction. the iberian peninsula still maintains one of the largest wolf (canis lupus) populations in europe. in iberia, sarcoptic mange is endemic in red foxes (vulpes vulpes) and the first confirmed wolf mange cases were recent ...201121600696
sarcoptic mange in iberian wolves (canis lupus signatus) in northern spain. 200818540036
to eat or not to eat? the diet of the endangered iberian wolf (canis lupus signatus) in a human-dominated landscape in central portugal.livestock predation by large carnivores and their persecution by local communities are major conservation concerns. in order to prevent speculations and reduce conflicts, it is crucial to get detailed and accurate data on predators' dietary ecology, which is particularly important in human dominated landscapes where livestock densities are high. this is the case of the endangered iberian wolf in portugal, an endemic subspecies of the iberian peninsula, which has seen its population distribution ...201526030294
high seroprevalence of antibodies to toxoplasma gondii in wild animals from portugal.we report an investigation of antibodies to toxoplasma gondii in 52 wild birds and 20 wild mammals from northern and central areas of portugal by using the modified agglutination test. the birds comprised 26 common buzzards (buteo buteo), five tawny owls (strix aluco), four white storks (ceconia ceconia), three eurasian eagle owls (bubo bubo), three northern goshawks (accipiter gentilis), two booted eagles (hieraaetus pennatus), two common barn owls (tyto alba), two eurasian sparrowhawks (accipi ...201021104273
detection of vancomycin-resistant enterococci from faecal samples of iberian wolf and iberian lynx, including enterococcus faecium strains of cc17 and the new singleton st573.the aim of this study was to perform the molecular characterization of vancomycin resistant enterococci (vre) within the faecal flora of iberian wolf and iberian lynx. the association with other resistance genes and the detection of virulence genes were also analysed. from 2008 to 2010, 365 faecal samples from iberian wolf and iberian lynx were collected and tested for vre recovery. mechanisms of resistance to vancomycin and other antibiotics, as well as genes encoding virulence factors were det ...201122018960
a selective sweep of >8 mb on chromosome 26 in the boxer genome.abstract:201121722374
using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the iberian wolf and leptospires.the iberian wolf (canis lupus) is the top predator in the iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. therefore, the wolf can serve as sentinel of environmental contamination with pathogens. we investigated the exposure of free-living wolves to 14 serovars of leptospira interrogans sensu lato. kidney samples from 49 wolves collected from 2010-2013 in northwestern spain were analysed by culture, direct immunofluores ...201425494467
prevalence of antibodies against canine distemper virus and canine parvovirus among foxes and wolves from spain.viral diseases can influence the population dynamics of wild carnivores and can have effects on carnivore conservation. hence, a serologic survey was conducted in an opportunistic sample of 137 foxes (vulpes vulpes) and 37 wolves (canis lupus) in spain for 1997-2007 to detect antibodies against canine distemper virus (cdv) and against canine parvovirus (cpv) by indirect elisa. antibodies against cdv were detected in 18.7% of the analyzed animals and antibodies against cpv in 17.2%. there was no ...200817646060
iberian wolf as a reservoir of extended-spectrum β-lactamase-producing escherichia coli of the tem, shv, and ctx-m groups.the intensive use of antibiotics in human and veterinary medicine, associated with mechanisms of bacterial genetic transfer, caused a selective pressure that contributed to the dissemination of antimicrobial resistance in different bacteria groups and throughout different ecosystems. iberian wolf, due to his predatory and wild nature, may serve as an important indicator of environmental contamination with antimicrobial resistant bacteria. the aim of this study was to characterize the diversity o ...201122185366
concomitance and interactions of pathogens in the iberian wolf (canis lupus).with the aim of improving our understanding of their epidemiological features, exposure to or presence of canine parvovirus (cpv), canine distemper virus (cdv), leishmania infantum and sarcoptes scabiei were studied in 88 wild wolves from asturias (northern spain) by means of long-term (2004-2010) serological and molecular data. individual and population factors and the possible interactions between them were also statistically analyzed for better understanding the contact/presence of studied pa ...201526267084
parasite species of the endangered iberian wolf (canis lupus signatus) and a sympatric widespread carnivore.parasites have a profound impact on wildlife population dynamics. however, until some years ago, studies on the occurrence and prevalence of wildlife parasites were neglected comparatively with the studies on humans and domestic animals. in this study, we determined the parasite prevalence of two sympatric wild canids: the endangered iberian wolf (canis lupus signatus) and the widespread red fox (vulpes vulpes), in central portugal. from november 2014 to july 2015, fresh fecal samples from both ...201627358768
patterns of exposure of iberian wolves (canis lupus) to canine viruses in human-dominated landscapes.wildlife inhabiting human-dominated landscapes is at risk of pathogen spill-over from domestic species. with the aim of gaining knowledge in the dynamics of viral infections in iberian wolves (canis lupus) living in anthropized landscapes of northern spain, we analysed between 2010 and 2013 the samples of 54 wolves by serology and polymerase chain reaction (pcr) for exposure to four pathogenic canine viruses: canine distemper virus (cdv), canine parvovirus-2 (cpv), canine adenovirus 1 and 2 (cav ...201626589403
viral gut metagenomics of sympatric wild and domestic canids, and monitoring of viruses: insights from an endangered wolf population.animal host-microbe interactions are a relevant concern for wildlife conservation, particularly regarding generalist pathogens, where domestic host species can play a role in the transmission of infectious agents, such as viruses, to wild animals. knowledge on viral circulation in wild host species is still scarce and can be improved by the recent advent of modern molecular approaches. we aimed to characterize the fecal virome and identify viruses of potential conservation relevance of diarrheic ...201728649326
antimicrobial resistance in faecal enterococci and escherichia coli isolates recovered from iberian wolf.the aim of this study was to report the antimicrobial resistance, the molecular mechanisms associated and the detection of virulence determinants within faecal enterococcus spp. and escherichia coli isolates of iberian wolf. enterococci (n = 227) and e. coli (n = 195) isolates were obtained from faecal samples of iberian wolf (canis lupus signatus). high rates of resistance were detected for tetracycline and erythromycin among the enterococci isolates, and most of resistant isolates harboured th ...201323336611
genetic epidemiology of sarcoptes scabiei in the iberian wolf in asturias, spain.during the last decades, attempts have been made to understand the molecular epidemiology of sarcoptes scabiei, and to detect and clarify the differences between isolates from different hosts and geographic regions. two main phenomena have been described: (i) host-taxon derived-sarcoptes mite infection in european wild animals (revealing the presence of three separate clusters, namely herbivore-, carnivore- and omnivore-derived sarcoptes populations in europe) and (ii) prey-to-predator sarcoptes ...201323664709
noninvasive heavy metal pollution assessment by means of iberian wolf (canis lupus signatus) hair from galicia (nw spain): a comparison with invasive samples.the current study represents the first investigation of the suitability of wolf hair as indicator tissue for metal exposure and accumulation within nw spanish ecosystems. dead animals, which were not specifically killed for these purposes, were necropsied, and further toxicological analyses were performed in order to establish the heavy metal content (pb, cd, and zn) in liver, kidney, and hair, by means of inductively coupled plasma optical emission spectrometry. two different factors, gender an ...201323877576
decoding group vocalizations: the acoustic energy distribution of chorus howls is useful to determine wolf reproduction.population monitoring is crucial for wildlife management and conservation. in the last few decades, wildlife researchers have increasingly applied bioacoustics tools to obtain information on several essential ecological parameters, such as distribution and abundance. one such application involves wolves (canis lupus). these canids respond to simulated howls by emitting group vocalizations known as chorus howls. these responses to simulated howls reveal the presence of wolf litters during the bre ...201627144887
relationships between sex and stress hormone levels in feces and marking behavior in a wild population of iberian wolves (canis lupus signatus).feces deposited by the breeding alpha pair on exposed substrates and/or zones may act as visual and olfactory marks associated with social dominance in wolves. the aim of this study was to determine if there was a correlation between marking behavior, sex hormone levels, and physiological stress in a wild population of iberian wolves in northwest spain. the glucocorticoid and sex hormone levels were measured in feces collected as a function of exposure (conspicuous/inconspicuous), height (above ...200818392658
quantification of sexual steroid hormones in faeces of iberian wolf (canis lupus signatus): a non-invasive sex typing method.the determination of gender in wild animals is essential for behavioural and ecological studies, and also for conservation. the objectives of this study were (i) the determination of gender in faecal samples of iberian wolf based on the differential concentrations of sexual steroid hormones (ssh) and (ii) to analyse the profiles of ssh in males and females (considering the gender determination carried out previously) during the non-reproductive and reproductive periods. the quantification of and ...200818422862
comparative proteomics of an extended spectrum β-lactamase producing escherichia coli strain from the iberian wolf.the iberian wolf (canis lupus signatus) is an endangered species native to the iberian peninsula. due to their predatory and wild nature, these wolves serve as important indicators of environmental contamination by antimicrobial-resistant bacteria. β-lactam antibiotics like cefotaxime are the most commonly used antibacterial agents. bacterial resistance to these antibiotics occurs predominantly through enzymatic inactivation by extended-spectrum beta-lactamases. escherichia coli strain wa57, iso ...201424631823
new insights into the genetic composition and phylogenetic relationship of wolves and dogs in the iberian peninsula.this study investigates the gene pool of portuguese autochthonous dog breeds and their wild counterpart, the iberian wolf subspecies (canis lupus signatus), using standard molecular markers. a combination of paternal and maternal molecular markers was used to investigate the genetic composition, genetic differentiation and genetic relationship of native portuguese dogs and the iberian wolf. a total of 196 unrelated dogs, including breed and village dogs from portugal, and other dogs from spain a ...201728649351
taeniid species of the iberian wolf (canis lupus signatus) in portugal with special focus on echinococcus spp.taeniid species represent relevant pathogens in human and animals, circulating between carnivorous definitive hosts and a variety of mammalian intermediate hosts. in portugal, however, little is known about their occurrence and life cycles, especially in wild hosts. an epidemiological survey was conducted to clarify the role of the iberian wolf as a definitive host for taeniid species, including echinococcus spp. wolf fecal samples (n = 68) were collected from two regions in northern portugal. t ...201324533315
plant-based remedies for wolf bites and rituals against wolves in the iberian peninsula: therapeutic opportunities and cultural values for the conservation of biocultural diversity.combined approaches to local knowledge and folk plant use improve awareness and promote effective strategies for the conservation of significant biocultural patrimony. moreover, the information reported might be the basis for further appropriate phytochemical and pharmacological research. therefore we provide an insight into traditional herbal remedies and practices for healing bite injuries in humans and domestic animals caused by the iberian wolf. wolf bites are associated with inflammatory pr ...201728755969
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