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cytophotometric and biochemical analyses of dna in pentaploid and diploid agave species.nuclear dna content, chromatin structure, and dna composition were investigated in four agave species: two diploid, agave tequilana weber and agave angustifolia haworth var. marginata hort., and two pentaploid, agave fourcroydes lemaire and agave sisalana perrine. it was determined that the genome size of pentaploid species is nearly 2.5 times that of diploid ones. cytophotometric analyses of chromatin structure were performed following feulgen or dapi staining to determine optical density profi ...199618469892
characterization of five typical agave plants used to produce mezcal through their simple lipid composition analysis by gas chromatography.five agave plants typically used in mexico for making mezcal in places included in the denomination of origin (mexican federal law that establishes the territory within which mezcal can be produced) of this spirit were analyzed: agave salmiana ssp. crassispina, a. salmiana var. salmiana, agave angustifolia, agave cupreata, and agave karwinskii. fatty acid and total simple lipid profiles of the mature heads of each plant were determined by means of a modified bligh-dyer extraction and gas chromat ...200919216532
diversity and structure of landraces of agave grown for spirits under traditional agriculture: a comparison with wild populations of a. angustifolia (agavaceae) and commercial plantations of a. tequilana.traditional farming communities frequently maintain high levels of agrobiodiversity, so understanding their agricultural practices is a priority for biodiversity conservation. the cultural origin of agave spirits (mezcals) from west-central mexico is in the southern part of the state of jalisco where traditional farmers cultivate more than 20 landraces of agave angustifolia haw. in agroecosystems that include in situ management of wild populations. these systems, rooted in a 9000-year-old tradit ...200921628200
the pollination biology of two paniculate agaves (agavaceae) from northwestern mexico: contrasting roles of bats as pollinators.paniculate agaves from tropical deserts depend on nectar-feeding bats for their reproduction, while species from extratropical areas near the northern limit of leptonycteris curasoae are pollinated by diurnal and nocturnal insects and birds. agave angustifolia is a paniculate agave with a broad distribution in mexico whose range coincides with the distribution of l. curasoae, while a. subsimplex has a narrow distribution in sonora within the range of nectar-feeding bats. we studied the pollinati ...200321659200
reproductive biology of henequen (agave fourcroydes) and its wild ancestor agave angustifolia (agavaceae). i. gametophyte development.the pathways of micro- and megagametophyte development in agave fourcroydes (henequén) and a. angustifolia were studied. we used histology and light microscopy to observe anther ontogeny and ovary differentiation in relation to flower bud size. both species have the same sexual reproductive strategies and gametophyte development that may be divided into three phases: (1) premeiotic, which includes the establishment of the megaspore mother cell and the pollen mother cell; (2) meiotic, the formati ...200121669630
Effects of Different Sources of Fructans on Body Weight, Blood Metabolites and Fecal Bacteria in Normal and Obese non-diabetic and Diabetic Rats.Fructans contribute significantly to dietary fiber with beneficial effects on gastrointestinal physiology in healthy individuals and offer a promising approach to treating some diseases. Two experiments (Experiment 1 = rats with normal weight; Experiment 2 = obese rats) were developed to compare the effects of three fructan sources (Cichorium intybus L. Asteraceae, Helianthus tuberosus L. Asteraceae and Agave angustifolia ssp. tequilana Haw, Agavaceae) on body weight change, blood metabolites an ...201122210166
an exploratory study into the putative prebiotic activity of fructans isolated from agave angustifolia and the associated anticancer activity.linear inulin-type fructan (itf) prebiotics have a putative role in the prevention of colorectal cancer, whereas relatively little is known about branched fructans. this study aims to investigate the fermentation properties and potential prebiotic activity of branched fructans derived from agave angustifolia haw, using the simulator of human intestinal microbial ecosystem (shime) model. the proximal, transverse and distal vessels were used to investigate fructan fermentation throughout the colon ...201323714623
anti-inflammatory activity of different agave plants and the compound cantalasaponin-1.species of the agave genus, such as agave tequilana, agave angustifolia and agave americana are used in mexican traditional medicine to treat inflammation-associated conditions. these plants' leaves contain saponin compounds which show anti-inflammatory properties in different models. the goal of this investigation was to evaluate the anti-inflammatory capacity of these plants, identify which is the most active, and isolate the active compound by a bio-directed fractionation using the ear edema ...201323846754
meiotic restitution mechanisms involved in the formation of 2n pollen in agave tequilana weber and agave angustifolia haw.a cytological analysis of the microsporogenesis was carried out in the agave tequilana and a. angustifolia species. several abnormalities such as chromosomal bridges, lagging chromosomes, micronuclei, monads, dyads and triads were found. the morphological analysis of the pollen, together with the above-mentioned 2n microspores, allowed us to confirm the presence of 2n pollen as well as its frequency. in both a. tequilana and a. angustifolia two different mechanisms were observed: the first mecha ...201223961348
physical mapping of 5s and 18s ribosomal dna in three species of agave (asparagales, asparagaceae).agave linnaeus, 1753 is endemic of america and is considered one of the most important crops in mexico due to its key role in the country's economy. cytogenetic analysis was carried out in agave tequilana weber, 1902 'azul', agave cupreata trelease et berger, 1915 and agave angustifolia haworth, 1812. the analysis showed that in all species the diploid chromosome number was 2n = 60, with bimodal karyotypes composed of five pairs of large chromosomes and 25 pairs of small chromosomes. furthermore ...201324260700
nocturnal versus diurnal co2 uptake: how flexible is agave angustifolia?agaves exhibit the water-conserving crassulacean acid metabolism (cam) photosynthetic pathway. some species are potential biofuel feedstocks because they are highly productive in seasonally dry landscapes. in plants with cam, high growth rates are often believed to be associated with a significant contribution of c3 photosynthesis to total carbon gain when conditions are favourable. there has even been a report of a shift from cam to c3 in response to overwatering a species of agave. we investig ...201424648568
prebiotic potential of agave angustifolia haw fructans with different degrees of polymerization.inulin-type fructans are the most studied prebiotic compounds because of their broad range of health benefits. in particular, plants of the agave genus are rich in fructans. agave-derived fructans have a branched structure with both β-(2→1) and β-(2→6) linked fructosyl chains attached to the sucrose start unit with a degree of polymerization (dp) of up to 80 fructose units. the objective of this work was to assess the prebiotic potential of three agave angustifolia haw fructan fractions (aff) wi ...201425153877
fungal planet description sheets: 214-280.novel species of microfungi described in the present study include the following from south africa: cercosporella dolichandrae from dolichandra unguiscati, seiridium podocarpi from podocarpus latifolius, pseudocercospora parapseudarthriae from pseudarthria hookeri, neodevriesia coryneliae from corynelia uberata on leaves of afrocarpus falcatus, ramichloridium eucleae from euclea undulata and stachybotrys aloeticola from aloe sp. (south africa), as novel member of the stachybotriaceae fam. nov. s ...201425264390
anti-inflammatory effect of 3-o-[(6'-o-palmitoyl)-β-d-glucopyranosyl sitosterol] from agave angustifolia on ear edema in mice.in mexico agave angustifolia has traditionally been used to treat inflammation. the aim of this study was to measure the anti-inflammatory effect of the extract of a. angustifolia, the isolation and identification of active compounds. from the acetone extract two active fractions were obtained, (asf13 and aaf16). for the characterization of pharmacological activity, the acute inflammatory model of mouse ear edema induced with tpa was used. the tissue exposed to tpa and treatments were subjected ...201425268718
agavins from agave angustifolia and agave potatorum affect food intake, body weight gain and satiety-related hormones (glp-1 and ghrelin) in mice.agavins act as a fermentable dietary fiber and have attracted attention due to their potential for reducing the risk of disease. therefore, we evaluated the effect of supplementation using 10% agavins with a short-degree of polymerization (sdp) from agave angustifolia haw. (aasdp) or agave potatorum zucc. (apsdp) along with chicory fructans (rse) as a reference for 5 weeks, on the energy intake, body weight gain, satiety-related hormones from the gut and blood (glp-1 and ghrelin), blood glucose ...201425367106
genomic diversity of saccharomyces cerevisiae yeasts associated with alcoholic fermentation of bacanora produced by artisanal methods.bacanora is a spirituous beverage elaborated with agave angustifolia haw in an artisanal process. natural fermentation is mostly performed with native yeasts and bacteria. in this study, 228 strains of yeast like saccharomyces were isolated from the natural alcoholic fermentation on the production of bacanora. restriction analysis of the amplified region its1-5.8s-its2 of the ribosomal dna genes (rflpr) were used to confirm the genus, and 182 strains were identified as saccharomyces cerevisiae. ...201525561061
hydrophobic modification of cellulose isolated from agave angustifolia fibre by graft copolymerisation using methyl methacrylate.graft copolymerisation of methyl methacrylate (mma) onto agave angustifolia was conducted with ceric ammonium nitrate (can) as the redox initiator. the maximum grafting efficiency was observed at can and mma concentrations of 0.91 × 10(-3) and 5.63 × 10(-2)m, respectively, at 45°c for 3h reaction time. four characteristic peaks at 2995, 1738, 1440, and 845 cm(-1), attributed to pmma, were found in the ir spectrum of grafted cellulose. the crystallinity index dropped from 0.74 to 0.46, while the ...201525857961
studies on the molluscicidal activity of agave angustifolia and pittosporum tobira on schistosomiasis transmitting snails.in the search for new molluscicidal plants for controlling the snail vectors of schistosomiasis, laboratory evaluation was made to assess the molluscicidal activity of agave angustifolia and pittosporum tobira plants against biomphalaria alexandrina snails. results indicated that both plants have promising molluscicidal activity as the lc90 of the dry powder of both plants was 120 ppm. both plants showed marked cercaricidal and miracidicidal potencies against s. mansoni larvae. the lc90 of both ...201526012228
identification of the temperature induced larvicidal efficacy of agave angustifolia against aedes, culex, and anopheles larvae.synthetic insecticides are generally employed to control the mosquito population. however, their injudicious over usage and non-biodegradability are associated with many adverse effects on the environment and mosquitoes. the application of environment-friendly mosquitocidals might be an alternate to overcome these issues. in this study, we found that organic or aqueous extracts of agave angustifolia leaves exhibited a strong larvicidal activity (ld50 28.27 μg/ml) against aedes aegypti, culex qui ...201626793700
morphological features of different polyploids for adaptation and molecular characterization of cc-nbs-lrr and lea gene families in agave l.polyploidy has been widely described in many agave l. species, but its influence on environmental response to stress is still unknown. with the objective of knowing the morphological adaptations and regulation responses of genes related to biotic (lea) and abiotic (nbs-lrr) stress in species of agave with different levels of ploidy, and how these factors contribute to major response of agave against environmental stresses, we analyzed 16 morphological trials on five accessions of three species ( ...201627016883
physiological differences and changes in global dna methylation levels in agave angustifolia haw. albino variant somaclones during the micropropagation process.global dna methylation changes caused by in vitro conditions are associated with the subculturing and phenotypic variation in agave angustifolia haw. while the relationship between the development of albinism and in vitro culture is well documented, the role of epigenetic processes in this development leaves some important questions unanswered. during the micropropagation of agave angustifolia haw., we found three different phenotypes, green (g), variegated (v) and albino (a). to understand the ...201627590059
erratum to: physiological differences and changes in global dna methylation levels in agave angustifolia haw. albino variant somaclones during the micropropagation process. 201727999975
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