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an identification in fish of the genus puntius hamilton 1822 (cypriniformes: cyprinidae) of some wetlands in northeast thailand with the use of random amplified polymorphic dna technique.the experiment was carried out during the 2003 to 2006 at the department of fisheries, khon kaen university, khon kaen, thailand in collaboration with the department of biosciences, the university of hertfordshire, college land, hatfield, herts, uk. molecular rapd technique was used for the determinations of dna patterns of the fish genus puntius hamilton 1822. the fish samples of 1,500 individual fish were collected from fifteen wetlands in northeast thailand and they were used for dna extracti ...200818817121
a morphological identification in fish of the genus puntius hamilton 1822 (cypriniformes: cyprinidae) of some wetlands in northeast thailand.this investigation was carried out at the department of fisheries, khon kaen university, khon kaen, thailand in collaboration with the department of biosciences, university of hertfordshire, hatfield, united kingdom. the work was carried out in the 2003 to 2006 to taxonomically identify fish of the genus puntius hamilton 1822 harvested from 15 wetlands in northeast thailand. some considerable amounts of fish samples were collected and used for morphological characteristics identifications both m ...200719093500
myxozoan pathogens of malaysian fishes cultured in ponds and net-cages.we describe new myxosporean species from malaysian fishes cultured in pond farms and net-cages. myxobolus omari sp. nov. and m. leptobarbi sp. nov. were found in the muscles of pangasianodon hypophthalmus and leptobarbus hoevenii, respectively, while plasmodia and spores of thelohanellus zahrahae sp. nov. and henneguya daoudi sp. nov. were detected in the gills of barbonymus gonionotus and trichogaster trichopterus, respectively. plasmodia and spores found in these fishes differed from the known ...200919301636
identification of novel and zoonotic cryptosporidium species in fish from papua new guinea.there is still limited information on the distribution of cryptosporidium species and genotypes in fish. the present study investigated the prevalence of cryptosporidium species in cultured freshwater (n=132), wild freshwater (n=206) and wild marine (n=276) fish in papua new guinea (png) by pcr screening at the 18s rrna locus. a total of seven fish (2 cultured freshwater, 1 wild freshwater and 4 wild marine fish) were identified as positive for cryptosporidium. specifically, cryptosporidium was ...201324064001
trans- and cis-urocanic acid, biogenic amine and amino acid contents in ikan pekasam (fermented fish) produced from javanese carp (puntius gonionotus) and black tilapia (oreochromis mossambicus).ikan pekasam is a fermented fish product produced in malaysia and is usually made from freshwater fish with ground roasted uncooked rice as the main source of carbohydrate. in this study, the amino acid, biogenic amine, and trans- and cis-urocanic acid (uca) contents of fifteen commercial samples of ikan pekasam made from javanese carp and black tilapia, that had undergone either natural or acid-assisted fermentation, were quantified. the latter includes either tamarind (tamarindus indica) pulp ...201525442635
alteration of blood parameters and histoarchitecture of liver and kidney of silver barb after chronic exposure to quinalphos.quinalphos (qp) is commonly used for pest control in the agricultural fields surrounding freshwater reservoirs. this study was conducted to evaluate the chronic toxicity of this pesticide on blood parameters and some organs of silver barb, barbonymus gonionotus. fish were exposed to two sublethal concentrations, 0.47 ppm and 0.94 ppm, of qp for a period of 28 days. all the blood parameters (red blood cell, hematocrit, and hemoglobin) and blood glucose except for white blood cells decreased with ...201526635877
acute exposure to a quinalphos containing insecticide (convoy) causes genetic damage and nuclear changes in peripheral erythrocytes of silver barb, barbonymus gonionotus.the present study was aimed to assess the genotoxic effect in fish caused by convoy, an insecticide commercial formulation containing quinalphos, present in the aquatic waterbody. for this purpose a freshwater teleost, silver barb was exposed to sublethal concentrations (25% and 50% of lc50) of convoy and erythrocytic cellular abnormalities (eca) and erythrocytic nuclear abnormalities (ena) tests were performed in addition to the commonly used micronucleus (mn) assay using peripheral erythrocyte ...201627667680
dietary fish oil replacement by linseed oil: effect on growth, nutrient utilization, tissue fatty acid composition and desaturase gene expression in silver barb (puntius gonionotus) fingerlings.silver barb (puntius gonionotus) is considered a promising medium carp species for freshwater aquaculture in asia. this study in silver barb was carried out to evaluate the effects of total or partial substitution of dietary fish oil (fo) with linseed oil (lo) on growth, nutrient utilization, whole-body composition, muscle and liver fatty acid composition. fish (12.1±0.4g of initial body weight) were fed for 60days with five experimental iso-proteinous, iso-lipidic and iso-caloric diets in which ...201727913275
alterations of organ histopathology and metallothionein mrna expression in silver barb, puntius gonionotus during subchronic cadmium exposure.common silver barb, puntius gonionotus, exposed to the nominal concentration of 0.06 mg/l cd for 60 d, were assessed for histopathological alterations (gills, liver and kidney), metal accumulation, and metallothionein (mt) mrna expression. fish exhibited pathological symptoms such as hypertrophy and hyperplasia of primary and secondary gill lamellae, vacuolization in hepatocytes, and prominent tubular and glomerular damage in the kidney. in addition, kidney accumulated the highest content of cad ...200718232229
histopathological effects of paraquat and gill function of puntius gonionotus, bleeker. 19873801702
determination of levels of mn, as, and other metals in water, sediment, and biota from phayao lake, northern thailand, and assessment of dietary exposure.this study was undertaken to determine levels of contamination of toxic metals in water, sediment, and consumed fishery products from phayao freshwater lake located in northern thailand, which is a major water resource for drinking water, agriculture, and household use. concentrations of mn, as, and other metals were determined in water, sediment, fish tissues (puntius gonionotus) and pond snails (filopaludina martensi). sampling was carried out in 3 periods (february, may, and august) in 2005. ...200717616874
comparison of infectivity, metacercarial burden and host mortality induced by opisthorchis viverrini sensu lato cercariae from lao pdr compared with thailand in cyprinid fish, barbonymus gonionotus.previous studies indicate that opisthorchis viverrini sensu lato (s.l.) is a species complex with strong associations to geographical regions, i.e., specific wetlands. the present study was conducted to compare the infectivity, establishment and metacercarial burden and survival of the fish host following exposure to o. viverrini s.l. cercariae isolates from two different river wetlands.201626740362
infectivity and development of opisthorchis viverrini metacercariae in immunosuppressed barbonymus gonionotus fingerlings (cyprinidae).opisthorchiasis caused by opisthorchis viverrini infection, is a serious public health problem in lao pdr, cambodia, vietnam and thailand. cyprinid fish are required for the transmission of o. viverrini metacercariae to fish eating-mammal hosts including humans. the transmission of infective metacerariae is an important event, potentially resulting in the development of cholangiocarcinoma in cases of chronic opisthorchiasis. for a better understanding of the development of o. viverrini metacerca ...201627338181
ddt and derivatives in indicator species of the aquatic food web of rangsit agricultural area, central thailand.the presence of ddt and derivatives in the food web of freshwater ecosystems of rangsit agricultural area, pathum thani province, thailand were investigated from june 2004 to may 2007. by using gas chromatography (gc) with micro electron capture detector (mu ecd), ddt and derivatives in water, sediment, and fifteen indicator species i.e., 2 producers; eichhornia crassipes and plankton (phyto- and zoo- plankton), an herbivore; trichogaster microlepis (3) 3 omnivores; trichogaster trichopterus, or ...200920161116
a cross-sectional study on the potential transmission of the carcinogenic liver fluke opisthorchis viverrini and other fishborne zoonotic trematodes by aquaculture fish.throughout southeast asia and china, eating raw and or partially cooked cyprinid fish causes liver (hepatobiliary) disease and cancer (cholangiocarcinoma) due to fishborne zoonotic trematodes (fzt), in particular clonorchis sinensis and opisthorchis viverrini. the primary source of transmission is by native fish, but aquaculture fish are also reported to have high infective potential. here, a cross-sectional survey of fzt in fish farms was conducted in an endemic area in khon kaen province, thai ...201323320421
Effects of Bayluscide on Bithynia siamensis goniomphalos, the first intermediate host of the human liver fluke, Opisthorchis viverrini, in laboratory and field trials.The molluscicidal effects of Bayluscide (niclosamide) were investigated on Bithynia siamensis goniomphalos, the first intermediate host of human liver fluke, Opisthorchis viverrini. Lethal concentrations of 50% (LC(50)) and 95% (LC(95)) against young and adult males were 0.38 and 0.80, 0.42 and 0.86ppm, respectively. The LC(50) and LC(95) against young and adult females were 0.42 and 0.86, 0.46 and 0.97ppm, respectively. No significant differences in mortality rate between sexes or snail size (p ...201221864711
ultrastructural changes in various organs of the fish puntius gonionotus fed cadmium-enriched cyanobacteria.the accumulation and toxicity of cadmium in puntius gonionotus fish that consumed the cyanobacterium spirulina platensis contaminated with cadmium were studied. fish were fed cadmium-contaminated cells for 4 weeks, after which cadmium accumulation in various organs was determined. the highest cadmium content was found in the kidney (56.0 microg cd/g wet weight). cadmium was not detected in the gill during the entire 4 weeks of cadmium feeding. histopathological alteration of cells was observed i ...200415526263
histopathological changes in the gastrointestinal tract of fish, puntius gonionotus, fed on dietary cadmium. 200314567583
intensity of trematode metacercariae in cyprinoid fish in chiang mal province, northern thailand.the intensity of infection of trematode metacercariae was determined in five species of cyprinoid fish collected from mae ngud reservoir, chiang mai province. these species were thynnichthys thynnoides, puntioplites proctozysron, hampala macrolepidota, puntius leiacanthus and puntius gonionotus. t. thynnoides contained the highest number (83.0%) of metacercariae, whereas p. gonionotus had the fewest (0.19%). the caudal fin was the area of highest infection of metacercariae (49.39%), with the ana ...200112041593
effect of macrophages and serum of fish susceptible or resistant to epizootic ulcerative syndrome (eus) on the eus pathogen, aphanomyces invadans.epizootic ulcerative syndrome (eus) is one of the most destructive diseases of fresh and brackish water farmed and wild fish in the asia-pacific region. the in vitro germination and growth of the propagules of the eus pathogen, aphanomyces invadans (= a. piscicida), were assessed in the presence of the head-kidney macrophages, serum, and serum heated to inactivate complement proteins, of three eus-susceptible and one resistant fish species. the susceptible species were: striped snakehead (channa ...200111592585
immunological parameters of javanese carp puntius gonionotus (bleeker) exposed to copper and challenged with aeromonas hydrophila.this study was to determine the median lethal concentration (lc50) of copper to javenese carp, puntius gonionotus (bleeker), and the immune response after the fish were exposed to sublethal levels of copper and challenged with formalin killed aeromonas hydrophila. the lc50 of copper on p. gonionotus at 24, 48, 72, 96 and 120 h were estimated as 2.17, 0.91, 0.57, 0.53 and 0.42 mg l(-1), respectively. to determine the effect of copper on the immune system, fish were exposed for 66 days to 0.05, 0. ...200111417716
application of hazard analysis critical control point (haccp) as a possible control measure for opisthorchis viverrini infection in cultured carp (puntius gonionotus).opisthorchiasis due to opisthorchis viverrini and transmitted through infected freshwater cyprinoid fish (carps) affects more than 8 million people in thailand, people's democratic republic of lao, and vietnam. the hazard analysis critical control point (haccp)-concept has been recommended by fao and who to be included in programs to control foodborne trematode infections (fbt). haccp is a multifactorial approach to control food hazards through surveillance of diseases, foods, and operations and ...19979656352
experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus.opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24mm) of barbonymus gonionotus fish on infection success of o. viver ...201424780189
reprint of "experimental infection of opisthorchis viverrini cercariae to the cyprinid fish, barbonymus gonionotus".opisthorchis viverrini is an important public health problem, a major cause of cholangiocarcinoma in the greater mekong subregion including lao pdr, cambodia, vietnam and thailand. humans acquire the infection by consumption of raw, fermented or partially cooked freshwater cyprinid fish containing infective metacercariae. this study examined the effect of cercarial infection dosage (25-200 cercariae), age (1-60 day) and size (1-24 mm) of barbonymus gonionotus fish on infection success of o. vive ...201525446173
susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to haplorchis taichui.fish-borne zoonotic trematodes (fzt) including heterophyids and opisthorchiids are prevalent in native and aquaculture fish in southeast asia. fzt are reported highly prevalent in juvenile aquaculture fish that belonged to the family heterophyidae, particularly haplorchis taichui. aquaculture fish are reported to have varying levels of natural infection with h. taichui, but data for their susceptibility as well as resistance to infection are not available. the aim of this study was to determine ...201525636245
occurrence of some medically important flukes (trematoda: opisthorchiidae and heterophyidae) in nam ngum water reservoir, laos.occurrence of some medically important flukes transmitted to man by eating of raw fish was observed at 18 localities of nam ngum water reservoir, an important source of fish for inhabitants of vientiane province, laos. cercariae of opisthorchis viverrini were recorded in 0.9% of bithynia siamensis goniomphalus examined but in the most populated sites, where the density of the snails was the highest up to 2.2% of the intermediate hosts were infected. haplorchis cercariae were found in 2.0% of tar ...19902075492
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