diversity of acetic acid bacteria present in healthy grapes from the canary islands.the identification of acetic acid bacteria (aab) from sound grapes from the canary islands is reported in the present study. no direct recovery of bacteria was possible in the most commonly used medium, so microvinifications were performed on grapes from tenerife, la palma and lanzarote islands. up to 396 aab were isolated from those microvinifications and identified by 16s rrna gene sequencing and phylogenetic analysis. with this method, acetobacter pasteurianus, acetobacter tropicalis, glucono ...201121903289
efficient production of 2,5-diketo-d-gluconate via heterologous expression of 2-ketogluconate dehydrogenase in gluconobacter japonicus.2,5-diketo-d-gluconate (2,5dkg) is a compound that can be the intermediate for d-tartrate and also vitamin c production. although gluconobacter oxydans nbrc3293 produces 2,5dkg from d-glucose via d-gluconate and 2-keto-d-gluconate (2kg), with accumulation of the product in the culture medium, the efficiency of 2,5dkg production is unsatisfactory because there is a large amount of residual d-gluconate at the end of the biotransformation process. oxidation of 2kg to 2,5dkg is catalyzed by a membra ...201525769838
impact of gluconic fermentation of strawberry using acetic acid bacteria on amino acids and biogenic amines profile.this paper studies the amino acid profile of beverages obtained through the fermentation of strawberry purée by a surface culture using three strains belonging to different acetic acid bacteria species (one of gluconobacter japonicus, one of gluconobacter oxydans and one of acetobacter malorum). an hplc-uv method involving diethyl ethoxymethylenemalonate (deemm) was adapted and validated. from the entire set of 21 amino acids, multiple linear regressions showed that glutamine, alanine, arginine, ...201525704705
comparison of d-gluconic acid production in selected strains of acetic acid bacteria.the oxidative metabolism of acetic acid bacteria (aab) can be exploited for the production of several compounds, including d-gluconic acid. the production of d-gluconic acid in fermented beverages could be useful for the development of new products without glucose. in the present study, we analyzed nineteen strains belonging to eight different species of aab to select those that could produce d-gluconic acid from d-glucose without consuming d-fructose. we tested their performance in three differ ...201626848948
effect of ammonium and amino acids on the growth of selected strains of gluconobacter and acetobacter.acetic acid bacteria (aab) are a group of microorganisms highly used in the food industry. however, its use can be limited by the insufficient information known about the nutritional requirements of aab for optimal growth. the aim of this work was to study the effects of different concentrations and sources of nitrogen on the growth of selected aab strains and to establish which nitrogen source best encouraged their growth. two strains of three species of aab, gluconobacter japonicus, gluconobac ...201727870985
identification of acetic acid bacteria through matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and report of gluconobacter nephelii kommanee et al. 2011 and gluconobacter uchimurae tanasupawat et al. 2012 as later heterotypic synonyms of gluconobacter japonicus malimas et al. 2009 and gluconobacter oxydans (henneberg 1897) de ley 1961 (approved lists 1980) emend. gosselé et al. 1983, respectively.the classification and identification of acetic acid bacteria (aab) underwent much change during the last two decades. however, several closely related aab species are difficult to differentiate and rapid and correct species level identification remains challenging. in the present study, 281 well-characterized aab strains representing aab species of the family acetobacteraceae were used to construct a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) ide ...201728237188
draft genome sequences of gluconobacter cerinus cect 9110 and gluconobacter japonicus cect 8443, acetic acid bacteria isolated from grape must.we report here the draft genome sequences of gluconobacter cerinus strain cect9110 and gluconobacter japonicus cect8443, acetic acid bacteria isolated from grape must. gluconobacter species are well known for their ability to oxidize sugar alcohols into the corresponding acids. our objective was to select strains to oxidize effectively d-glucose.201627365351
heterologous overexpression and characterization of a flavoprotein-cytochrome c complex fructose dehydrogenase of gluconobacter japonicus nbrc3260.a heterotrimeric flavoprotein-cytochrome c complex fructose dehydrogenase (fdh) of gluconobacter japonicus nbrc3260 catalyzes the oxidation of d-fructose to produce 5-keto-d-fructose and is used for diagnosis and basic research purposes as a direct electron transfer-type bioelectrocatalysis. the fdhscl genes encoding the fdh complex of g. japonicus nbrc3260 were isolated by a pcr-based gene amplification method with degenerate primers designed from the amino-terminal amino acid sequence of the l ...201223275508
gluconobacter japonicus sp. nov., an acetic acid bacterium in the alphaproteobacteria.five strains, nbrc 3271(t), nbrc 3272, nbrc 3263, nbrc 3260 and nbrc 3269 were examined genetically, phylogenetically, phenotypically and chemotaxonomically. the dna g+c contents of the five strains were 55.1-56.4 mol%. the five strains had low levels of dna-dna hybridization of 13-51 % to the type strains of gluconobacter frateurii, gluconobacter thailandicus, gluconobacter oxydans, gluconobacter cerinus, gluconobacter albidus and gluconobacter kondonii and formed a cluster that was separate fr ...200919244423
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