| construction of green fluorescent protein-tagged recombinant iridovirus to assess viral replication. | green fluorescent protein-tagged recombinant virus has been successfully applied to observing the infective dynamics and evaluating viral replication. here, we identified soft-shelled turtle iridovirus (stiv) orf55 as an envelope protein (vp55), and developed a recombinant stiv expressing an enhanced green fluorescent protein (egfp) fused to vp55 (egfp-stiv). recombinant egfp-stiv shared similar single-step growth curves and ultrastructural morphology with wild type stiv (wt-stiv). the green flu ... | 2011 | 21756948 |
| ifn-γ in turtle: conservation in sequence and signalling and role in inhibiting iridovirus replication in chinese soft-shelled turtle pelodiscus sinensis. | the ifn-γ gene was identified in a turtle, the chinese soft-shelled turtle, pelodiscus sinensis, with its genome consisting of 4 exons and 3 introns. the deduced amino acid sequence of this gene contains a signal peptide, an ifn-γ family signature motif (130)iqrkavnelfpt, an nls motif (155)krkr and three potential n-glycosylation sites. as revealed by real-time quantitative pcr, the gene was constitutively expressed in all tested organs/tissues, with higher level observed in blood, intestine and ... | 2014 | 24239708 |
| characterization of dna aptamers generated against the soft-shelled turtle iridovirus with antiviral effects. | soft-shelled turtle iridovirus (stiv) causes severe systemic disease in farmed soft-shelled turtles (trionyx sinensis). more efficient methods of controlling and detecting stiv infections are urgently needed. | 2015 | 26419355 |
| selection and characterization of single-chain recombinant antibodies against infectious haematopoietic necrosis virus from mouse phage display library. | six single-chain fragment variable (scfv) antibodies against infectious haematopoietic necrosis virus (ihnv) were selected from an antibody phage display library by phage display technology. the soluble scfv antibodies showed a molecular weight 32kda by western blot. dot blot analysis revealed that the six scfv antibodies could recognize ihnv. for enzyme linked immunosorbent assay (elisa), four scfv antibodies (p1a4, p1a12, p1d5 and p3e2) showed cross-reactivity with spring viraemia of carp viru ... | 2014 | 24747010 |
| establishment of a new cell line from the heart of giant grouper, epinephelus lanceolatus (bloch), and its application in toxicology and virus susceptibility. | a new marine fish cell line, derived from the heart of giant grouper, epinephelus lanceolatus (bloch), was established and characterized. the cell line was designated as elgh and subcultured with more than 60 passages. the elgh cells were mainly composed of fibroblast-like cells and multiplied well in leibovitz's l-15 medium supplemented with 10% foetal bovine serum (fbs) at 28 °c. chromosome analysis indicated that the modal chromosome number was 48. the fluorescent signals were detected in elg ... | 2015 | 24372271 |
| a loop-mediated isothermal amplification method for the detection of members of the genus ranavirus. | a loop-mediated isothermal amplification (lamp) method was developed for detection of members of the genus ranavirus. the optimum reaction mixture contained 2.5 μl of each inner primer, rv-fip (20 pmol/μl) and rv-bip (20 pmol/μl), 0.5 μl of each outer primer, rv-f3 (10 pmol/μl) and rv-b3 (10 pmol/μl), 1.25 μl of each loop primer, rv-lf (20 pmol/μl) and rv-lb (20 pmol/μl), 3.5 μl dntp mix (10 mm each), 8 μl mgso4 (25 mm), 1 μl of bst dna polymerase (8 u/ml, large fragment; new england biolabs inc ... | 2013 | 23665768 |
| identification and determination of antigenic proteins of korean ranavirus-1 (krv-1) using maldi-tof/tof ms analysis. | ranaviruses are serious pathogens of fish, amphibians, and reptiles, and pose a major threat to global biodiversity. a ranavirus isolated from tissues of diseased tadpoles and frogs in gangwon province, korea, in 2006 and 2007, was designated korean ranavirus-1 (krv-1) and was infectious in a variety of fish cell lines with highest titers (10(10)tcid(50)/ml) in epithelioma papulosum cyprini cells (epcs) and baby hamster kidney-21 (bhk-21) cells. bullfrog (rana catesbeiana) tadpoles challenged by ... | 2011 | 21388683 |
| a double antibody sandwich enzyme-linked immunosorbent assay for detection of soft-shelled turtle iridovirus antigens. | a double antibody sandwich enzyme-linked immunosorbent assay (das-elisa) for detection of the soft-shelled turtle iridovirus (stiv) was developed using a specific monoclonal antibody (mab) against stiv and anti-stiv rabbit serum. using das-elisa, the detection limit of stiv was found to be 10(3)pfu/ml. the positive rate of 15 stiv samples was 100%, while the positive rate of 100 other aquatic virus samples was 0%. these data show that das-elisa is highly specific and sensitive for the detection ... | 2010 | 20399233 |
| development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of lymphocystis disease virus. | a loop-mediated isothermal amplification (lamp) assay was developed for the detection of lymphocystis disease virus (lcdv). a set of five specific primers, two inner and two outer primers and a loop primer, were designed on the basis of the major capsid protein gene of lcdv. the reaction time and temperatures were optimized for 60 min at 63 degrees c, respectively. lamp amplification products were detected by a ladder-like appearance on agarose gel electrophoresis or a naked-eye inspection of a ... | 2010 | 19891986 |
| complete sequence determination of a novel reptile iridovirus isolated from soft-shelled turtle and evolutionary analysis of iridoviridae. | soft-shelled turtle iridovirus (stiv) is the causative agent of severe systemic diseases in cultured soft-shelled turtles (trionyx sinensis). to our knowledge, the only molecular information available on stiv mainly concerns the highly conserved stiv major capsid protein. the complete sequence of the stiv genome is not yet available. therefore, determining the genome sequence of stiv and providing a detailed bioinformatic analysis of its genome content and evolution status will facilitate furthe ... | 2009 | 19439104 |
| characterization of a late gene encoding for mcp in soft-shelled turtle iridovirus (stiv). | major caspid protein (mcp) is the major structural component of virus particles and revealed to be very responsible for classification of new tentative iridovirus isolates. in this paper, the complete sequences of mcp gene was firstly identified and characterized from soft-shelled turtle iridovirus (stiv). the mcp, classified as a late transcript by drug inhibition, encodes a protein of 463 aa with a predicted molecular weight of 50kda. indirect immunofluorescence (iif) and virus neutralization ... | 2007 | 17706827 |
| involvement of the mitogen-activated protein kinase pathway in soft-shelled turtle iridovirus-induced apoptosis. | iridoviruses are large dna viruses that infect invertebrates and poikilothermic vertebrates, and result in significant economic losses in aquaculture production, and drastic declines in amphibian populations. soft-shelled turtle iridovirus (stiv) is the causative agent of severe systemic diseases in farm-raised soft-shelled turtles (trionyx sinensis). in the present study, the mechanisms of stiv-induced cell death and the roles of the mitogen-activated protein kinase (mapk) signaling pathway wer ... | 2011 | 21442306 |
| evaluation of a loop-mediated isothermal amplification assay for rapid diagnosis of soft-shelled turtle iridovirus. | softshelled turtle iridovirus (stiv) is the first asian iridovirus isolated from reptiles, which infects soft-shelled turtles severely and leads to "red neck disease" associated with high mortality. a set of four specific primers was designed by targeting the stiv thymidine kinase (tk) gene and amplified stiv dna specifically under optimized amplification conditions at 63°c for 60 min. the sensitivity of the loop-mediated isothermal amplification (lamp) assay was found to be 20 copies/μl of stiv ... | 2011 | 21392535 |
| a new iridovirus isolated from soft-shelled turtle. | a virus was isolated from soft-shelled turtle (trionyx sinensis) with 'red neck disease' on a farm in shenzhen, china, the virus multiplied and caused a cytopathogenic effect (cpe) at 15-30 degrees c in co, fhm, ck and bf-2 cells. the optimum conditions for replication was in co cells at 25-30 degrees c. the virus was sensitive to chloroform treatment, acid (ph 3) or alkaline (ph 10) conditions and heating at 56 degrees c for 30 min. treatment with 5-iodo-2-deoxyuridine (iudr) inhibited viral re ... | 1999 | 10509727 |
| rapid diagnosis of largemouth bass ranavirus in fish samples using the loop-mediated isothermal amplification method. | largemouth bass ranavirus (lmbv) has been recognized as the causative pathogen responsible for infectious skin ulcerative syndrome in cultured largemouth bass in china. a fast and simple lmbv detection method is urgently needed. here, a loop-mediated isothermal amplification (lamp) assay was established for the detection of this virus using primers targeting the major capsid protein gene of lmbv. the amplification conditions were optimized; the assay was specific for the diagnosis of lmbv, as th ... | 2020 | 32268179 |
| establishment and characterization of a new cell line derived from kidney of grouper, epinephelus akaara (temminck & schlegel), susceptible to singapore grouper iridovirus (sgiv). | a marine fish cell line derived from the kidney of red-spotted grouper, epinephelus akaara, designated as eagk was established and characterized. the eagk cells multiplied well in leibovitz's l-15 medium containing 10% foetal bovine serum at 25ôçâ-¦c and have been subcultured for more than 90 passages. karyotyping, chromosomal typing and ribosomal rna (rrna) genotyping analysis revealed that eagk had a modal diploid chromosome number of 82 and was a fibroblast cell line originated from grouper. ... | 2011 | 21838711 |