| enzootic activity of pixuna and rio negro viruses (venezuelan equine encephalitis complex) in a neotropical region of argentina. | venezuelan equine encephalitis complex viruses cause epidemics and epizootics periodically in some regions of the americas. in argentina, only enzootic rio negro virus (ag80-663) (rnv) has been isolated. to survey and identify activity of viruses that belong to venezuelan equine encephalitis complex in a template region of the country, a generic alphavirus rt-nested pcr was performed in 99 mosquito pools collected in chaco province. five pools were positive, and amplicons were sequenced: four of ... | 2010 | 19402767 |
| evolutionary genetics and vector adaptation of recombinant viruses of the western equine encephalitis antigenic complex provides new insights into alphavirus diversity and host switching. | western equine encephalitis virus (weev), highlands j virus (hjv), and fort morgan virus (fmv) are the sole representatives of the wee antigenic complex of the genus alphavirus, family togaviridae, that are endemic to north america. all three viruses have their ancestry in a recombination event involving eastern equine encephalitis virus (eeev) and a sindbis (sin)-like virus that gave rise to a chimeric alphavirus that subsequently diversified into the present-day weev, hjv, and fmv. here, we pr ... | 2014 | 25463613 |
| in- silico exploration of thirty alphavirus genomes for analysis of the simple sequence repeats. | the compilation of simple sequence repeats (ssrs) in viruses and its analysis with reference to incidence, distribution and variation would be instrumental in understanding the functional and evolutionary aspects of repeat sequences. present study encompasses the analysis of ssrs across 30 species of alphaviruses. the full length genome sequences, assessed from ncbi were used for extraction and analysis of repeat sequences using imex software. the repeats of different motif sizes (mono- to penta ... | 2014 | 25606453 |
| isolation and characterisation of a human-like antibody fragment (scfv) that inactivates veev in vitro and in vivo. | venezuelan equine encephalitis virus (veev) belongs to the alphavirus genus and several species of this family are pathogenic to humans. the viruses are classified as potential agents of biological warfare and terrorism and sensitive detection as well as effective prophylaxis and antiviral therapies are required.in this work, we describe the isolation of the anti-veev single chain fragment variable (scfv), tor67-3b4, from a non-human primate (nhp) antibody gene library. we report its recloning i ... | 2012 | 22666347 |
| coi barcode versus morphological identification of culex (culex) (diptera: culicidae) species: a case study using samples from argentina and brazil. | sequences of the cytochrome c oxidase subunit i (coi) mitochondrial gene from adults of 22 culex (culex) species from argentina and brazil were employed to assess species identification and to test the usefulness of coi for barcoding using the best close match (bcm) algorithm. a pairwise kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 coi barcode sequences was constructed. of the 12 coi lineages recovered in the neighbour-joining topology, five co ... | 0 | 24473810 |
| sindbis and middelburg old world alphaviruses associated with neurologic disease in horses, south africa. | old world alphaviruses were identified in 52 of 623 horses with febrile or neurologic disease in south africa. five of 8 sindbis virus infections were mild; 2 of 3 fatal cases involved co-infections. of 44 middelburg virus infections, 28 caused neurologic disease; 12 were fatal. middelburg virus likely has zoonotic potential. | 0 | 26583836 |
| cocirculation of rio negro virus (rnv) and pixuna virus (pixv) in tucumán province, argentina. | venezuelan equine encephalitis complex includes viruses considered emerging pathogens for humans and animals in the americas. two members of this complex have been detected previously in argentina: rio negro virus (rnv), detected in mosquitoes from chaco province and rodents from formosa province, and pixuna virus (pixv), detected in mosquitoes from chaco province. to carry out surveillance studies in other parts of the country, detection of a 195-bp fragment of alphaviruses by rt-nested pcr was ... | 2010 | 20497404 |
| venezuelan equine encephalitis viruses (veev) in argentina: serological evidence of human infection. | venezuelan equine encephalitis viruses (veev) are responsible for human diseases in the americas, producing severe or mild illness with symptoms indistinguishable from dengue and other arboviral diseases. for this reason, many cases remain without certain diagnosis. seroprevalence studies for veev subtypes iab, id, if (mosso das pedras virus; mdpv), iv (pixuna virus; pixv) and vi (rio negro virus; rnv) were conducted in persons from northern provinces of argentina: salta, chaco and corrientes, u ... | 2013 | 24349588 |
| first detection of rio negro virus (venezuelan equine encephalitis complex subtype vi) in córdoba, argentina. | rio negro virus (rnv) (venezuelan equine encephalitis subtype vi) circulates only in argentina; in northern provinces, isolates have been obtained from mosquitoes and rodents since 1980 and have been associated with acute febrile illness in humans. however, no studies of rnv have been performed in the central area of the country. we carried out molecular and serological detection of rnv in córdoba, a province of the central part of the country, in mosquitoes and humans, respectively. one mosquit ... | 2012 | 22310545 |
| specific detection of all members of the venezuelan equine encephalitis complex: development of a rt-nested pcr. | venezuelan equine encephalitis (vee) complex belongs to alphavirus genus in the family togaviridae. several species of this complex are pathogenic to humans. vee infections can produce severe or mild disease, and many cases remain undiagnosed. a specific and sensitive reverse transcriptase nested polymerase chain reaction (rt-nested pcr) method was developed for the detection of all vee subtypes, including rio negro virus (rnv) (subtype vi), which circulates only in argentina. degenerated primer ... | 2012 | 22609888 |
| genetic and evolutionary characterization of venezuelan equine encephalitis virus isolates from argentina. | venezuelan equine encephalitis viruses (veev) are emerging pathogens of medical and veterinary importance circulating in america. argentina is a country free from epizootic veev activity, with circulation of enzootic strains belonging to rio negro virus (rnv; veev subtype vi) and pixuna virus (pixv, veev subtype iv). in this work, we aim to report the sequencing and phylogenetic analyses of all argentinean vee viruses, including 7 strains previously isolated from mosquitoes in 1980, 5 sequences ... | 2014 | 24833218 |
| pan-genome analysis of brazilian lineage a amoebal mimiviruses. | since the recent discovery of samba virus, the first representative of the family mimiviridae from brazil, prospecting for mimiviruses has been conducted in different environmental conditions in brazil. recently, we isolated using acanthamoeba sp. three new mimiviruses, all of lineage a of amoebal mimiviruses: kroon virus from urban lake water; amazonia virus from the brazilian amazon river; and oyster virus from farmed oysters. the aims of this work were to sequence and analyze the genome of th ... | 2015 | 26131958 |
| niemeyer virus: a new mimivirus group a isolate harboring a set of duplicated aminoacyl-trna synthetase genes. | it is well recognized that gene duplication/acquisition is a key factor for molecular evolution, being directly related to the emergence of new genetic variants. the importance of such phenomena can also be expanded to the viral world, with impacts on viral fitness and environmental adaptations. in this work we describe the isolation and characterization of niemeyer virus, a new mimivirus isolate obtained from water samples of an urban lake in brazil. genomic data showed that niemeyer harbors du ... | 2015 | 26635738 |