| genetic markers in ribosomal dna for the identification of members of the genus anisakis (nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. | polymerase-chain-reaction-based restriction fragment length polymorphism analysis was performed to establish genetic markers in rdna, for the identification of the three sibling species of the anisakis simplex complex and morphologically differentiated anisakis species, i.e. anisakis physeteris, anisakis schupakovi, anisakis typica and anisakis ziphidarum. different restriction patterns were found between a. simplex sensu stricto and anisakis pegreffii with two of the restriction endonucleases u ... | 2000 | 10704605 |
| molecular characterization of larval anisakid nematodes from marine fishes of madeira by a pcr-based approach, with evidence for a new species. | one-hundred and fifteen anisakid larvae from 3 different fish hosts, aphanopus carbo, scomber japonicus, and trachurus picturatus, caught in madeiran waters, were identified by pcr-rflp. three distinct species were identified in a. carbo, namely anisakis simplex sensu srricto, anisakis pegreffii, and anisakis ziphidarum; 5 in s. japonicus, i.e., a. simplex s.s., a. pegreffii, anisakis physeteris, anisakis typica, and a. ziphidarum; and 3 in t. picturatus, i.e., a. simplex s.s., a. pegreffii, and ... | 2005 | 16539027 |
| risk factors for human anisakis infection and association between the geographic origins of scomber japonicus and anisakid nematodes. | it has been reported that nearly all cases of anisakiasis in japan are caused by anisakis simplex sensu stricto. to elucidate this further, we investigated the presence of anisakis type i larvae and pseudoterranova decipiens in 218 scomber japonicus fish collected from the seas of japan. anisakis type i larvae were detected in 74.3% (162/218) of the fish, and 99.8% of the anisakis type i larvae comprised a. simplex sensu stricto and anisakis pegreffii. molecular identification techniques were us ... | 2010 | 19892425 |
| molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern atlantic coasts of usa, gulf of mexico, and caribbean sea. | in the present study, 407 anisakid nematodes, collected from 11 different species of cetaceans of the families delphinidae, kogiidae, physeteridae, and ziphiidae, from the southeastern atlantic coasts of usa, the gulf of mexico, and the caribbean sea, were examined morphologically and genetically characterized by pcr restriction fragment length polymorphism to identify them to species level, assess their relative frequencies in definitive hosts, and determine any host preference. sequence data f ... | 2011 | 21212982 |
| morphological and molecular characterization of anisakis larvae (nematoda: anisakidae) in beryx splendens from japanese waters. | the third-stage (l3) larvae of anisakis, which are the etiological agents of human anisakiasis, have been categorized morphologically into anisakis type i larvae and anisakis type ii larvae. genetic analysis has allowed easy identification of these larvae: anisakis type i larvae include the species anisakis simplex sensu stricto, anisakis pegreffii, anisakis simplex c, anisakis typica, anisakis ziphidarum, and anisakis nascettii, whereas anisakis type ii larvae include the species anisakis physe ... | 2011 | 21377540 |
| Infection levels and diversity of anisakid nematodes in blackspot seabream, Pagellus bogaraveo, from Portuguese waters. | The blackspot seabream, Pagellus bogaraveo, is a sparid fish of great economic importance in the northeast Atlantic. The main aim of this work was to assess the infection levels and diversity of anisakid nematodes parasitizing P. bogaraveo from Portuguese waters. The anisakid larvae were identified by polymerase chain reaction-restriction fragment length polymorphism analysis and ten different patterns were observed, four of which were not previously reported in the literature. Moreover, several ... | 2011 | 22139402 |
| anisakis spp. larvae in three mesopelagic and bathypelagic fish species of the central mediterranean sea. | in this work 437 fish samples of species belonging to the families myctophidae (electrona risso and diaphus metopoclampus) and phosichthyidae (vinciguerria attenuata) were examined for the presence of anisakidae larvae. the study was performed with fishes in the central mediterranean sea, particularly in the strait of sicily and in the strait of messina. the visual inspection and chloro-peptic analysis revealed the presence of nematode parasites with prevalence values between 2.9% in electrona r ... | 2018 | 28965943 |
| integrative taxonomy of anisakid nematodes in stranded cetaceans from brazilian waters: an update on parasite's hosts and geographical records. | anisakidae are represented mainly by species of anisakis dujardin, 1845, pseudoterranova krabbe, 1878, and contracaecum railliet and henry, 1913. integrative taxonomy, based on morphological and genetic approaches, can provide a precise identification of species, increasing the knowledge of their systematics, biology, and evolution. in this study, integrative taxonomy was applied in order to identify, at generic and/or specific taxonomic level, 245 anisakids from 63 cetaceans of 12 different spe ... | 2017 | 28975458 |