Publications

the complete genome sequence of a new necrovirus isolated from olea europaea l.	the complete nucleotide sequence of a virus isolated from olea europaea l. (gp isolate), previously identified as an isolate of tobacco necrosis virus d (tnv-d) based on its coat protein sequence, was determined. the viral rna genome consists of 3683 nucleotides and contains five open reading frames. the putative rna-dependent rna polymerase shows 91.2% amino acid identity with that of an isolate of olive latent virus 1 (olv-1) and the coat protein reveals highest sequence identity with that of ...	2004	15592885
specific amino acids of olive mild mosaic virus coat protein are involved on transmission by olpidium brassicae.	transmission of olive mild mosaic virus (ommv) is facilitated by olpidium brassicae (wor.) dang.. an ommv mutant (ommvl11) containing two changes in the coat protein (cp), asn to tyr at position 189 and ala to thr at position 216, has shown not to be olpidium brassicae-transmissible due to inefficient attachment of virions to zoospores. in this study, these amino acid changes were separately introduced into the ommv genome through site-directed mutagenesis, and the asn to tyr change showed to be ...	2011	21593272
a degenerate pair of primers for simultaneous detection of four alpha- and betanecroviruses.	the high infection levels due to olive latent virus 1 (olv-1), olive mild mosaic virus (ommv) (alphanecrovirus) and tobacco necrosis virus d (tnv-d) (betanecrovirus) in portuguese olive orchards prompted us to develop a rapid pcr-based assay for the simultaneous detection of these viruses aimed at the sanitary selection and marketing of plant material in compliance with european union regulations. a pair of degenerate oligonucleotide primers, parrdrp5' and parcoat3' was designed based on conserv ...	2014	25116202
genetic diversity of the coat protein of olive mild mosaic virus (ommv) and tobacco necrosis virus d (tnv-d) isolates and its structural implications.	the genetic variability among 13 isolates of olive mild mosaic virus (ommv) and of 11 isolates of tobacco necrosis virus d (tnv-d) recovered from olea europaea l. samples from various sites in portugal, was assessed through the analysis of the coat protein (cp) gene sequences. this gene was amplified through reverse transcriptase polymerase chain reaction (rt-pcr), cloned, and 5 clone sequences of each virus isolate, were analysed and compared, including sequences from ommv and tnv-d isolates or ...	2014	25350108
correction: genetic diversity of the coat protein of olive mild mosaic virus (ommv) and tobacco necrosis virus d (tnv-d) isolates and its structural implications.		2015	25955830