[pyoderma in dogs]. | bacterial infections associated with cutaneous commensal organisms like staphylococcus pseudintermedius are frequently encountered in small animal practice. in this review, pathogenesis, clinical signs, and topical and systemic therapeutic options are discussed. | 2011 | 22167166 |
antimicrobial activity of gallium maltolate against staphylococcus aureus and methicillin-resistant s. aureus and staphylococcus pseudintermedius: an in vitro study. | gallium is a trivalent semi-metallic element that has shown antimicrobial activity against several important human pathogens. this antimicrobial activity is likely related to its substitution in important iron-dependent pathways of bacteria. the genus staphylococcus, which includes human and animal pathogens that cause significant morbidity and mortality, requires iron for growth and colonization. in this study, gallium maltolate, at various concentrations between 50 and 200μm, inhibited the in ... | 2011 | 21963417 |
distribution of sccmec-associated phenol-soluble modulin in staphylococci. | the recently described phenol-soluble modulin psm-mec was detected in staphylococcus aureus, staphylococcus epidermidis, staphylococcus fleuretti, staphylococcus hominis, staphylococcus pseudintermedius, staphylococcus saprophyticus, staphylococcus simulans and staphylococcus vitulinus from different hosts (humans, goats, dogs, cats, pigs, cattle and turkeys). it was identified in isolates harbouring sccmec types ii, iia, iib, iid, iii, viii and in some irregular or truncated elements. | 2012 | 22251619 |
what's happened to staphylococcus intermedius? taxonomic revision and emergence of multi-drug resistance. | staphylococcus intermedius has been the predominant coagulase-positive staphylococcus isolated from canine skin and mucosae and the most commonly reported staphylococcal pathogen in small animal practice for the last 35 years. although microbiological tests have historically indicated variability in biochemical characteristics amongst s. intermedius isolates from animals, an acceptable level of diagnostic accuracy for clinical purposes was readily achievable with routine phenotypic testing. howe ... | 2012 | 22251285 |
methicillin-resistant staphylococcus pseudintermedius isolated from canine pyoderma in north china. | aims: to determine the prevalence of carriage of methicillin-resistant staphylococcus pseudintermedius (mrsp) among dogs with pyoderma from two small animal hospitals in north china during a 21-month period, and to characterize these isolates. methods and results: swabs were taken from 260 dogs with pyoderma, the staphylococcal species isolated and methicillin resistance were confirmed phenotypically and genotypically. the identified mrsp isolates were characterized by multilocus sequence typi ... | 2012 | 22229826 |
activity, expression and genetic variation of canine β-defensin 103: a multifunctional antimicrobial peptide in the skin of domestic dogs. | the skin functions as more than a physical barrier to infection. epithelial cells of the skin can synthesize antimicrobial peptides, including defensins, which exhibit direct antimicrobial activity. here we characterize the expression pattern, genetic variation and activity of the major β-defensin expressed in canine skin, canine β-defensin 103 (cbd103). the gene encoding cbd103 exhibits two forms of polymorphism: a common 3-basepair deletion allele and a gene copy-number variation. golden retri ... | 2012 | 22261569 |
insertion sequence is256 in canine pyoderma isolates of staphylococcus pseudintermedius associated with antibiotic resistance. | staphylococcus pseudintermedius is the most frequent staphylococcal species isolated from canine pyoderma. the control of s. pseudintermedius infection is often difficult due to the expanded antimicrobial resistance phenotypes. antibiotic resistance in staphylococcal pathogens is often associated to mobile genetic elements such as the insertion sequence is256 that was first described as a part of the transposon tn4001, which confers aminoglycoside resistance in staphylococcus aureus and in staph ... | 2011 | 22261238 |
necrotizing fasciitis caused by methicillin-resistant staphylococcus pseudintermedius at a previously irradiated site in a dog. | a great dane dog was presented with a small, superficial wound on the left tarsus that rapidly progressed to a large necrotic area. the dog had undergone radiation therapy in the left tarsal region 33 months previously. necrotizing fasciitis was diagnosed on histopathological examination, and bacterial culture revealed methicillin-resistant staphylococcus pseudintermedius. | 2012 | 23633717 |
identification and antibiotic susceptibility of bacterial isolates from dogs with otitis externa in australia. | to identify and quantify the five most frequently isolated significant bacterial microorganisms, and their antibiotic susceptibility, from bacterial cultures of samples taken from the ears of dogs with otitis externa. | 2012 | 23356371 |
molecular characterization of high-level mupirocin resistance in staphylococcus pseudintermedius. | the genetic analysis of high-level mupirocin resistance (hi-mup(r)) in a staphylococcus pseudintermedius isolate from a dog is presented. the hi-mup(r) iles2 gene flanked by a novel rearrangement of directly repeated insertion sequence is257 elements was located, together with the aminoglycoside resistance aaca-aphd determinant, on a conjugative plasmid related to the psk41/pgo1 family plasmids. | 2012 | 23269741 |
staphylococcus pseudintermedius and staphylococcus schleiferi subspecies coagulans from canine pyoderma cases in grenada, west indies, and their susceptibility to beta-lactam drugs. | over a 2-year period 66 cases of canine pyoderma in grenada, west indies, were examined by aerobic culture in order to ascertain the bacteria involved and their antimicrobial resistance patterns. of the 116 total bacterial isolates obtained, the majority belonged to gram-positive species, and the most common organism identified through biochemical and molecular methods was staphylococcus pseudintermedius. additionally, identification of a staphylococcus schleiferi subspecies coagulans isolate wa ... | 2014 | 24592351 |
systematic review on global epidemiology of methicillin-resistant staphylococcus pseudintermedius: inference of population structure from multilocus sequence typing data. | background and rationale: methicillin-resistant staphylococcus pseudintermedius (mrsp) is a major cause of infections in dogs, also posing a zoonotic risk to humans. this systematic review aimed to determine the global epidemiology of mrsp and provide new insights into the population structure of this important veterinary pathogen. methodology: web of science was searched systematically for articles reporting data on multilocus sequence typing (mlst) of s. pseudintermedius isolates from dogs or ... | 2016 | 27803691 |
canine influenza virus coinfection with staphylococcus pseudintermedius enhances bacterial colonization, virus load and clinical presentation in mice. | canine influenza virus (civ) and staphylococcus pseudintermedius (sp) are pathogens that cause respiratory disease in dogs. considering bacterial infections following influenza are a leading cause of illness and death, it is of particular meaning to investigate the interaction between these two pathogens. in this study, balb/c mice were used as a mouse model to assess whether inoculation with civ h3n2 followed by s. pseudintermedius 72 h later resulted in exacerbation of disease. disease was cha ... | 2016 | 27259293 |
optimization and evaluation of flexicult® vet for detection, identification and antimicrobial susceptibility testing of bacterial uropathogens in small animal veterinary practice. | urinary tract infection (uti) is a common reason for antimicrobial prescription in dogs and cats. the objective of this study was to optimize and evaluate a culture-based point-of-care test for detection, identification and antimicrobial susceptibility testing of bacterial uro-pathogens in veterinary practice. | 2015 | 26503106 |
characterisation of bacterial growth and antimicrobial susceptibility patterns in canine urinary tract infections. | bacterial urinary tract infection (uti) is a common reason for antimicrobial therapy in dogs. a reported increase in multi-drug resistance in canine bacterial pathogens, including resistance to extended-spectrum cephalosporins (esc) is of concern as antimicrobial resistance complicates therapy in dogs. in addition, it is a possible public health concern. the objectives of this study were to investigate the relative prevalence of pathogens in urine samples from dogs with urinary tract infection s ... | 2014 | 25249356 |
in vitro evaluation of dispersinb on methicillin-resistant staphylococcus pseudintermedius biofilm. | methicillin resistant staphylococcus pseudintermedius (mrsp) is an important canine pathogen that has been shown to produce biofilm in vitro. biofilm production may be an important virulence factor and methods to eliminate biofilm-associated infections are required. an enzyme of aggregatibacter actinomycetemcomitans, dispersinb, has been shown to degrade the extracellular matrix in the biofilm of a variety of bacteria including staphylococcus aureus and staphylococcus epidermidis. the objective ... | 2013 | 23932310 |
inactivation of microorganisms by low-frequency high-power ultrasound: 1. effect of growth phase and capsule properties of the bacteria. | the aim of this study was to determine the effects of high-intensity low-frequency (20 khz) ultrasound treatment on the viability of bacteria suspension. more specifically, we have investigated the relationship between the deactivation efficiency and the physical (size, hydrophobicity) and biological (gram-status, growth phase) properties of the microbes. enterobacter aerogenes, bacillus subtilis, staphylococcus epidermidis, s. epidermidis sk and staphylococcus pseudintermedius were chosen for t ... | 2014 | 23835398 |
susceptibility of canine and feline bacterial pathogens to pradofloxacin and comparison with other fluoroquinolones approved for companion animals. | in this study, 908 bacterial pathogens from defined infections of dogs and cats were tested for their susceptibility to the novel fluoroquinolone pradofloxacin, which was approved in 2011 for use in cats and dogs. most of the bacteria tested (staphylococcus aureus, staphylococcus pseudintermedius, escherichia coli, β-haemolytic streptococci, pasteurella multocida and bordetella bronchiseptica) exhibited low pradofloxacin mic(90) values of ≤ 0.25 μg/ml. solely proteus spp. and pseudomonas aerugin ... | 2013 | 22939523 |
canine antimicrobial peptides are effective against resistant bacteria and yeasts. | antimicrobial peptides (amps) are small proteins present in most living species and they are involved in the defence against pathogenic organisms. β-defensins and cathelicidin are the most frequently studied amps in both people and dogs. | 2014 | 24215268 |
sequence-based characterization of tn5801-like genomic islands in tetracycline-resistant staphylococcus pseudintermedius and other gram-positive bacteria from humans and animals. | antibiotic resistance in pathogens is often associated with mobile genetic elements, such as genomic islands (gi) including integrative and conjugative elements (ices). these can transfer resistance genes within and between bacteria from humans and/or animals. the aim of this study was to investigate whether tn5801-like gis carrying the tetracycline resistance gene, tet(m), are common in staphylococcus pseudintermedius from pets, and to do an overall sequences-based characterization of tn5801-li ... | 2016 | 27199912 |
determination of minimum biofilm eradication concentrations of orbifloxacin for canine bacterial uropathogens over different treatment periods. | biofilm formation can cause refractory urinary tract infections (utis) in dogs; however, minimum biofilm eradication concentrations (mbecs) of veterinary drugs against canine uropathogens remain to be investigated. in this study, the mbecs of orbifloxacin (obfx), trimethoprim-sulfamethoxazole (tms) and amoxicillin/clavulanate (acv) over different time periods for treatment of canine uropathogenic escherichia coli (n = 10) were determined. the mbecs of obfx for other bacterial uropathogens, inclu ... | 2017 | 28042660 |
antimicrobial susceptibility monitoring of dermatological bacterial pathogens isolated from diseased dogs and cats across europe (compath results). | the compath project is a pan-european programme dedicated to the monitoring of antimicrobial susceptibility of pathogens from diseased dogs and cats using standardized methods and centralized minimum inhibitory concentration (mic) determination. here, the susceptibility of major pathogens is reported from antimicrobial nontreated animals with acute clinical signs of skin, wound or ear infections in 2008-2010. | 2016 | 27593360 |
can ultraviolet light c decrease the environmental burden of antimicrobial-resistant and -sensitive bacteria on textiles? | the environment is important in transmission of bacteria. textiles are difficult and time consuming to clean; ultraviolet light c (uvc) is germicidal and may be an effective disinfection method for textile surfaces. | 2016 | 27620856 |
prevalence and resistance patterns of canine uropathogens in regard to concurrent diseases. | predisposing factors for different types of urinary tract infections (uti) were evaluated and prevalence of causative agents and their resistance were identified. a prospective epidemiologic study (2007 to 2012) included 191 dogs with signs of urinary tract disease. anamnestic data were collected and clinical examination, abdominal ultrasonography, urinalysis and aerobic bacteriologic urine culture were performed in all dogs. other diagnostic procedures were conducted when indicated. uti was mor ... | 2016 | 27529997 |
comparative in vitro killing of canine strains of staphylococcus pseudintermedius and escherichia coli by cefovecin, cefazolin, doxycycline and pradofloxacin. | bacterial eradication is necessary for clinical cure of infections and antimicrobial agents are important adjunctive therapies for inhibiting the growth of or killing bacteria. pre-existing skin diseases predispose animals to infection by staphylococcus pseudintermedius and, more rarely, by gram-negative bacilli. the property of rapid killing of bacteria may influence drug selection and duration of therapy in the setting of infection. | 2016 | 27307405 |
multidrug-resistant opportunistic pathogens challenging veterinary infection control. | although the problems associated with healthcare-associated infections (hai) and the emergence of zoonotic and multidrug-resistant pathogens in companion animal (dogs, cats and horses) medicine have been well-known for decades, current progress with respect to practical implementation of infection control programs in veterinary clinics has been limited. clinical outbreak events reported for methicillin-resistant staphylooccus aureus (mrsa) and staphylococcus pseudintermedius (mrsp), extended spe ... | 2017 | 27291944 |
a pilot study comparing in vitro efficacy of topical preparations against veterinary pathogens. | topical antimicrobial agents are important for the management of cutaneous infections. for topical antimicrobial agents, in vitro efficacy data are limited. | 2016 | 27109449 |
assessment of wound bio-burden and prevalence of multi-drug resistant bacteria during open wound management. | to describe the bacterial bio-burden of open-treated wounds and make comparisons with bite wounds. | 2016 | 27101415 |
clinical features, cytology and bacterial culture results in dogs with and without cheilitis and comparison of three sampling techniques. | cheilitis is a common presentation in dogs associated with a variety of skin diseases and often complicated by microbial infections. | 2016 | 27019393 |
is transmission electron microscopy (tem) a promising approach for qualitative and quantitative investigations of polymyxin b and miconazole interactions with cellular and subcellular structures of staphylococcus pseudintermedius, escherichia coli, pseudomonas aeruginosa and malassezia pachydermatis? | antimicrobial therapy using a combination of polymyxin b and miconazole is effective against the main bacterial pathogens associated with otitis externa in dogs, and a synergistic effect of both drugs has been shown previously. the objective of the present investigation was to visualize ultrastructural changes after exposure of escherichia coli, pseudomonas aeruginosa, staphylococcus pseudintermedius and malassezia pachydermatis to polymyxin b and miconazole by transmission electron microscopic ... | 2015 | 26527257 |
the distribution of pathogens and their antimicrobial susceptibility patterns among canine surgical wound infections in sweden in relation to different risk factors. | surgical site infection (ssi) is a common nosocomial infection in dogs and a growing concern in veterinary hospitals as an increase in multidrug-resistant pathogens is reported. despite the need for rational and prudent antimicrobial use, few peer-reviewed and published veterinary studies have investigated the pathogenic growth including susceptibility patterns of the isolated pathogens in canine ssis. the first objective of the present study was to estimate the distribution of bacterial pathoge ... | 2015 | 25886937 |
in vitro assessment of chloramphenicol and florfenicol as second-line antimicrobial agents in dogs. | the aim of this study was to evaluate the potential of chloramphenicol and florfenicol as second-line antimicrobial agents for treatment of infections caused by methicillin-resistant staphylococcus pseudintermedius (mrsp) and extended-spectrum β-lactamase (esbl)-producing escherichia coli in dogs, through a systematic in vitro assessment of the pharmacodynamic properties of the two drugs. minimum inhibitory concentrations (mic) and phenicol resistance genes were determined for 169 s. pseudinterm ... | 2015 | 25623169 |
the joint in vitro action of polymyxin b and miconazole against pathogens associated with canine otitis externa from three european countries. | canine otitis externa, an inflammation of the external ear canal, can be maintained and worsened by bacterial or fungal infections. for topical treatment, combinations of anti-inflammatory and antimicrobial ingredients are mainly used. | 2013 | 23721182 |
mutant prevention concentration of orbifloxacin: comparison between escherichia coli, pseudomonas aeruginosa, and staphylococcus pseudintermedius of canine origin. | the mutant prevention concentration (mpc) is an important parameter to evaluate the likelihood of growth of fluoroquinolone-resistant mutants for antimicrobial-pathogen combinations. the mpcs of fluoroquinolones for different canine pathogens have not been compared. in this study, we compared for the first time orbifloxacin mpcs between susceptible strains of escherichia coli, pseudomonas aeruginosa, and staphylococcus pseudintermedius of canine origin. | 2013 | 23634902 |
antimicrobial resistance in bacteria from breeding dogs housed in kennels with differing neonatal mortality and use of antibiotics. | this work examines the antimicrobial resistance of potentially pathogenic bacteria (staphylococcus pseudintermedius, streptococcus canis, escherichia coli) found in the vaginal tract in prepartum mammary secretions and postpartum milk of bitches housed in breeding kennels (n = 20; 92 bitches). the kennels were divided into three categories: no routine antimicrobial administration around parturition (category 1); routine administration of one antibiotic around parturition (category 2); routine ad ... | 2012 | 22898018 |
the in vitro activity of 15 antimicrobial agents against bacterial isolates from dogs. | the in vitro activity of 15 antimicrobial agents against clinical isolates of staphylococcus pseudintermedius, staphylococcus aureus, escherichia coli, pasteurella spp. and streptococcus canis from dogs was investigated. for staphylococcus spp., the highest frequency of resistance was observed for penicillin, followed by ampicillin, tetracycline and chloramphenicol. the highest frequency of resistance in e. coli isolates was recorded for tetracycline and streptomycin. pasteurella spp. and s. can ... | 2012 | 22516694 |
fibronectin binding proteins spsd and spsl both support invasion of canine epithelial cells by staphylococcus pseudintermedius. | in this study, we investigated the cell wall-anchored fibronectin-binding proteins spsd and spsl from the canine commensal and pathogen staphylococcus pseudintermedius for their role in promoting bacterial invasion of canine progenitor epidermal keratinocytes (cpek). invasion was examined by the gentamicin protection assay and fluorescence microscopy. an δspsd δspsl mutant of strain ed99 had a dramatically reduced capacity to invade cpek monolayers, while no difference in the invasion level was ... | 2015 | 26238710 |
staphylococcus pseudintermedius for camp-test. | camp test reliably detects listeria monocytogenes (lm) and streptococcus agalactiae (group b streptococcus, gbs); it is traditionally performed streaking the tested isolate perpendicularly to staphylococcus aureus (sa), provided that reference sa strains (that produce β-hemolysin) are used. in a zone of β-hemolysin activity, in fact, gbs and lm form typical arrow-shaped hemolytic areas. while sa production of the toxin is strain-dependent, however, that of staphylococcus pseudintermedius (sp), a ... | 2014 | 24817971 |
in vitro evaluation of the use of diluted sodium hypochlorite (bleach) against staphylococcus pseudintermedius, pseudomonas aeruginosa and malassezia pachydermatis. | | 2014 | 24589038 |
in vitro comparison of the effectiveness of polihexanide and chlorhexidine against canine isolates of staphylococcus pseudintermedius, pseudomonas aeruginosa and malassezia pachydermatis. | polihexanide (polyhexamethylene biguanide) is an antiseptic substance that plays a prominent role in the treatment of critically colonized or infected acute and chronic wounds in humans. | 2013 | 23789723 |
[staphylococcus pseudintermedius and pasteurella dagmatis associated in a case of community-acquired pneumonia]. | | 2012 | 22366256 |
antibacterial effect of n-acetylcysteine on common canine otitis externa isolates. | n-acetylcysteine (nac) has the potential to be a useful therapeutic agent for the treatment of otitis externa due to its antimicrobial and mucolytic properties, as well as its ability to disrupt bacterial biofilm. | 2016 | 27122224 |
antibiotic treatment of resistant infections in small animals. | there are few veterinary clinical studies to support a recommended use and dose for treating resistant bacterial infections in small animals. resistance against many common antibiotics is possible and a susceptibility test is advised. infections caused by pseudomonas aeruginosa presents a special problem. staphylococcus isolated from small animals is most likely to be staphylococcus pseudintermedius. the most important resistance mechanism for staphylococcus is methicillin resistance. the only a ... | 2013 | 23890241 |
synergistic hemolysis between β-lysin-producing staphylococcus species and rothia nasimurium in primary cultures of clinical specimens obtained from dogs. | nonhemolytic isolates of catalase-positive, gram-positive cocci were observed that produced strong synergistic hemolysis with colonies of staphylococcus pseudintermedius and staphylococcus schleiferi subsp. coagulans in primary cultures of clinical specimens from dogs. the isolates appeared similar to nonhemolytic staphylococci but were not identified with conventional biochemical tests or commercial biochemical test kits. partial 16s ribosomal rna gene sequences from each of 9 tested isolates w ... | 2014 | 24760132 |
a case of methicillin-resistant staphylococcus pseudintermedius (mrsp) pyoderma in a labrador retriever dog. | an 8-year-old, neutered male labrador retriever dog with generalized pruritis had a history of recurring atopic dermatitis and superficial pyoderma. cocci and yeast were found on cytology and methicillin-resistant staphylococcus pseudintermedius was cultured. a regimen of marbofloxacin, dexamethasone, ketoconazole, and cyclosporine in addition to bathing with 2% chlorhexidine shampoo resulted in marked improvement. | 2014 | 25392557 |
an investigation of resistance to β-lactam antimicrobials among staphylococci isolated from pigs with exudative epidermitis. | a high proportion of staphylococci isolated from pigs affected with exudative epidermitis were found to be resistant to β-lactam antimicrobials. the primary objective of this research was to investigate and characterize β-lactam resistance in staphylococcus hyicus, staphylococcus aureus and other staphylococci isolated from these pigs. | 2013 | 24131819 |
presence of environmental coagulase-positive staphylococci, their clonal relationship, resistance factors and ability to form biofilm. | coagulase-positive staphylococci (cops) are opportunistic pathogens carrying various mechanisms of resistance that have a large number of virulence factors, and whose ability to induce illness is associated with the host. this study aimed to investigate the presence of environmental coagulase-positive staphylococci, their susceptibility profile, clonal relationship and ability to form biofilm. the 16s rrna genes from cops isolates were analyzed, and their antibiotic susceptibility was evaluated ... | 2017 | 28017482 |
the immunopathogenesis of staphylococcal skin infections - a review. | staphylococcus aureus and s. pseudintermedius are the major causes of bacterial skin disease in humans and dogs. these organisms can exist as commensals on the skin, but they can also cause severe or even devastating infections. the immune system has evolved mechanisms to deal with pathogenic microorganisms and has strategies to combat bacteria of this type. what emerges is a delicate "peace" between the opposing sides, but this balance can be disrupted leading to a full blown "war". in the fero ... | 2016 | 27865269 |
comparison of the in vitro activity of five antimicrobial drugs against staphylococcus pseudintermedius and staphylococcus aureus biofilms. | resistance in canine pathogenic staphylococci is necessitating re-evaluation of the current antimicrobial treatments especially for biofilm-associated infections. long, repeated treatments are often required to control such infections due to the tolerance of bacteria within the biofilm. to comply with the goal of better antibiotic stewardship in veterinary medicine, the efficacies of the available drugs need to be directly assessed on bacterial biofilms. we compared the activities of amoxicillin ... | 2016 | 27531995 |
pathophysiological mechanisms of staphylococcus non-aureus bone and joint infection: interspecies homogeneity and specific behavior of s. pseudintermedius. | implicated in more than 60% of bone and joint infections (bjis), staphylococci have a particular tropism for osteoarticular tissue and lead to difficult-to-treat clinical infections. to date, staphylococcus aureus internalization in non-professional phagocytic cells (nppcs) is a well-explored virulence mechanism involved in bji chronicity. conversely, the pathophysiological pathways associated with staphylococcus non-aureus (sna) bjis have scarcely been studied despite their high prevalence. in ... | 2016 | 27462303 |
searching for beta-haemolysin hlb gene in staphylococcus pseudintermedius with species-specific primers. | the paper presents an analysis of 51 staphylococcus pseudintermedius clinically isolated strains from humans and from animals. staphylococcus pseudintermedius strains' ability to produce β-haemolysin was evaluated with phenotypic methods (hot-cold effect, reverse camp test). in order to determine the hlb gene presence (coding for β-haemolysin) in a genomic dna, pcr reactions were conducted with two different pairs of primers: one described in the literature for staphylococcus aureus and recommen ... | 2016 | 27086303 |
detection and sequence analysis of accessory gene regulator genes of staphylococcus pseudintermedius isolates. | staphylococcus pseudintermedius (sp) is the major pathogenic species of dogs involved in a wide variety of skin and soft tissue infections. the accessory gene regulator (agr) locus of staphylococcus aureus has been extensively studied, and it influences the expression of many virulence genes. it encodes a two-component signal transduction system that leads to down-regulation of surface proteins and up-regulation of secreted proteins during in vitro growth of s. aureus. the objective of this stud ... | 2015 | 27047173 |
trends and molecular mechanisms of antimicrobial resistance in clinical staphylococci isolated from companion animals over a 16 year period. | the objective of this study was to investigate the evolution of resistance to antimicrobials, corresponding mechanisms and molecular characteristics of staphylococcus spp., between 1999 and 2014. | 2016 | 26944924 |
first identification of methicillin-resistant staphylococcus pseudintermedius strains among coagulase-positive staphylococci isolated from dogs with otitis externa in trinidad, west indies. | otitis externa is a common inflammatory ear disease in dogs caused by a variety of pathogens, and coagulase-positive staphylococci are frequently isolated from such infections. | 2015 | 26715379 |
evaluation of oxacillin and cefoxitin disk and mic breakpoints for prediction of methicillin resistance in human and veterinary isolates of staphylococcus intermedius group. | staphylococcus pseudintermedius is a coagulase-positive species that colonizes the nares and anal mucosa of healthy dogs and cats. human infections with s. pseudintermedius range in severity from bite wounds and rhinosinusitis to endocarditis; historically, these infections were thought to be uncommon, but new laboratory methods suggest that their true incidence is underreported. oxacillin and cefoxitin disk and mic tests were evaluated for the detection of meca- or mecc-mediated methicillin res ... | 2016 | 26607988 |
linoleic acid salt with ultrapure soft water as an antibacterial combination against dermato-pathogenic staphylococcus spp. | skin colonization of staphylococcus spp. critically affects the severity of dermatitis in humans and animals. we examined different types of fatty acid salts for their antibacterial activity against staphylococcus spp. when used in ultrapure soft water (upsw). we also evaluated their therapeutic effect on a spontaneous canine model of dermatitis. | 2016 | 26606689 |
oxacillin sensitization of methicillin-resistant staphylococcus aureus and methicillin-resistant staphylococcus pseudintermedius by antisense peptide nucleic acids in vitro. | antibiotic resistance genes can be targeted by antisense agents, which can reduce their expression and thus restore cellular susceptibility to existing antibiotics. antisense inhibitors can be gene and pathogen specific, or designed to inhibit a group of bacteria having conserved sequences within resistance genes. here, we aimed to develop antisense peptide nucleic acids (pnas) that could be used to effectively restore susceptibility to β-lactams in methicillin-resistant staphylococcus aureus (m ... | 2015 | 26560174 |
epidemiology of methicillin resistant staphylococcus pseudintermedius in guide dogs in finland. | methicillin resistant staphylococcus pseudintermedius (mrsp) and staphylococcus aureus (mrsa) are common multi-drug resistant (mdr) bacteria in dogs. in 2012-2013 three dogs of the guide dog school of the finnish federation of the visually impaired were found to be mrsp positive. guide dogs have regular contact with each other during their first year of life and prolonged contact when in training. since dogs are placed in different parts of finland after training, there is a risk for national sp ... | 2015 | 26183814 |
antibacterial characterization of novel synthetic thiazole compounds against methicillin-resistant staphylococcus pseudintermedius. | staphylococcus pseudintermedius is a commensal organism of companion animals that is a significant source of opportunistic infections in dogs. with the emergence of clinical isolates of s. pseudintermedius (chiefly methicillin-resistant s. pseudintermedius (mrsp)) exhibiting increased resistance to nearly all antibiotic classes, new antimicrobials and therapeutic strategies are urgently needed. thiazole compounds have been previously shown to possess potent antibacterial activity against multidr ... | 2015 | 26086336 |
a bioengineered nisin derivative to control biofilms of staphylococcus pseudintermedius. | antibiotic resistance and the shortage of novel antimicrobials are among the biggest challenges facing society. one of the major factors contributing to resistance is the use of frontline clinical antibiotics in veterinary practice. in order to properly manage dwindling antibiotic resources, we must identify antimicrobials that are specifically targeted to veterinary applications. nisin is a member of the lantibiotic family of antimicrobial peptides that exhibit potent antibacterial activity aga ... | 2015 | 25789988 |
susceptibility in vitro of canine methicillin-resistant and -susceptible staphylococcal isolates to fusidic acid, chlorhexidine and miconazole: opportunities for topical therapy of canine superficial pyoderma. | increasing multidrug resistance amongst canine pathogenic staphylococci has renewed interest in topical antibacterial therapy for skin infections in the context of responsible veterinary prescribing. we therefore determined the activity in vitro of three clinically relevant topical agents and synergism between two of them against staphylococcus pseudintermedius and staphylococcus aureus. | 2015 | 25749003 |
anatomical patterns of colonization of pets with staphylococcal species in homes of people with methicillin-resistant staphylococcus aureus (mrsa) skin or soft tissue infection (ssti). | methicillin-resistant strains of staphylococcus aureus (mrsa), staphylococcus pseudintermedius (mrsp), and other pathogenic staphylococci can cause infections in companion animals and humans. identification of colonized animals is fundamental to research and practice needs, but harmonized methods have not yet been established. to establish the optimal anatomic site for the recovery of methicillin-resistant coagulase positive staphylococci (cps), survey data and swabs were collected from 196 pets ... | 2015 | 25623014 |
staphylococcus pseudintermedius can be misdiagnosed as staphylococcus aureus in humans with dog bite wounds. | the purpose of this study was to investigate whether s. pseudintermedius is misdiagnosed as s. aureus by clinical laboratories when isolated from humans with dog bite wounds. in addition, we attempted to determine whether s. pseudintermedius isolates related to dog bite wounds share phenotypic and genotypic traits. s. pseudintermedius was identified by pcr targeting the nuc gene. isolates were tested for antibiotic susceptibility using vetmic gp-mo microdilution panels. the occurrence of genes e ... | 2015 | 25532507 |
nasal carriage of coagulase positive staphylococci in patients of a primary-healthcare-center: genetic lineages and resistance and virulence genes. | staphylococcus aureus and staphylococcus pseudintermedius are highly important due to their capacity for producing diseases in humans and animals, respectively. the aim of the study was to investigate and characterize the coagulase positive staphylococcus (cops) carriage in a primary healthcare center population. | 2017 | 25459195 |
apo, zn2+-bound and mn2+-bound structures reveal ligand-binding properties of sita from the pathogen staphylococcus pseudintermedius. | the gram-positive bacterium staphylococcus pseudintermedius is a leading cause of canine bacterial pyoderma, resulting in worldwide morbidity in dogs. s. pseudintermedius also causes life-threatening human infections. furthermore, methicillin-resistant s. pseudintermedius is emerging, resembling the human health threat of methicillin-resistant staphylococcus aureus. therefore it is increasingly important to characterize targets for intervention strategies to counteract s. pseudintermedius infect ... | 2014 | 25311310 |
prevalence and risk factors for isolation of methicillin-resistant staphylococcus in dogs with keratitis. | to determine the prevalence of, and risk factors for, methicillin-resistant staphylococcus (mrs) isolation in dogs with naturally acquired bacterial keratitis. | 2015 | 25130050 |
amikacin resistance in staphylococcus pseudintermedius isolated from dogs. | staphylococcus pseudintermedius is the most common microorganism isolated from canine pyoderma and postoperative wound infections. the prevalence of methicillin-resistant s. pseudintermedius (mrsp) has increased, and recently, isolates that are resistant not only to methicillin but also to other classes of antibiotic drugs, including aminoglycosides, have become common. a total of 422 s. pseudintermedius isolates collected from 413 dogs were analyzed for amikacin and methicillin resistance using ... | 2014 | 25078911 |
nasal carriage of methicillin-resistant staphylococcus aureus (mrsa) among swiss veterinary health care providers: detection of livestock- and healthcare-associated clones. | we screened a total of 340 veterinarians (including general practitioners, small animal practitioners, large animal practitioners, veterinarians working in different veterinary services or industry), and 29 veterinary assistants for nasal carriage of methicillin-resistant staphylococcus aureus (mrsa) and staphylococcus pseudintermedius (mrsp) at the 2012 swiss veterinary annual meeting. mrsa isolates (n = 14) were detected in 3.8 % (95 % ci 2.1 - 6.3 %) of the participants whereas mrsp was not d ... | 2014 | 24973319 |
strain typing and antimicrobial susceptibility of methicillin-resistant coagulase-positive staphylococcal species in dogs and people associated with dogs in thailand. | this study was to investigate and to characterize methicillin-resistant coagulase-positive staphylococci (mrcops) harboring in dogs and people associated with dogs in thailand. | 2014 | 24833550 |
diversity and evolution of oligopeptide permease systems in staphylococcal species. | several oligopeptide permease (opp) systems have been found in staphylococcal species, including opp1-4, opp3' and the arginine catabolic mobile element (acme)-encoded opp system (acme-opp). they confer upon bacteria the increasing fitness, but their evolutionary histories remain unclear. in this work, we performed a genome-wide identification of opp systems in staphylococcal species. novel opp systems were identified, including the duplicate of opp4 in staphylococcus pseudintermedius and the ac ... | 2014 | 24793159 |
carriage of methicillin-resistant staphylococci by healthy companion animals in the us. | antimicrobial-resistant staphylococci have been associated with wounded or ill companion animals, but little is known about the prevalence of resistant staphylococci among healthy animals. in this study, 276 healthy dogs and cats from veterinary clinics were tested for the presence of antimicrobial-resistant staphylococcus spp. isolates were tested for antimicrobial susceptibility and the presence of select resistance genes, and typed using pulsed-field gel electrophoresis (pfge). staphylococcus ... | 2014 | 24730724 |
prevalence and characterization of staphylococcus aureus and staphylococcus pseudintermedius isolated from companion animals and environment in the veterinary teaching hospital in zambia, africa. | the republic of zambia consists of only one veterinary teaching school at the university of zambia (unza) where students and veterinarians are exposed to many bacterial pathogens including staphylococcus aureus (sa) and staphylococcus pseudintermedius (sp). the aim of this study was the characterization and antimicrobial susceptibility profile of eleven sa and 48 sp isolates from the veterinary hospitals' in- and outpatients and the environment. no isolate was resistant to cefoxitin by disk diff ... | 2014 | 24480623 |
small colony variant of methicillin-resistant staphylococcus pseudintermedius st71 presenting as a sticky phenotype. | we first observed the phenomenon of small colony variants (scvs) in a staphylococcus pseudintermedius sequence type 71 (st71) strain, isolated from a non-pet owner. although we found that small-sized colonies share main features with staphylococcus aureus scvs, they nevertheless show a novel, particular, and sticky phenotype, whose expression was extremely stable, even after subcultivation. | 2014 | 24452163 |
canine superficial bacterial folliculitis: current understanding of its etiology, diagnosis and treatment. | superficial bacterial folliculitis (sbf) is more common in the dog than other mammalian species. until recently, a successful outcome in cases of canine sbf was possible by administering a potentiated amoxicillin, a first generation cephalosporin or a potentiated sulfonamide. unfortunately, this predictable susceptibility has changed, because methicillin resistant staphylococcus pseudintermedius (mrsp) and staphylococcus aureus (mrsa) are becoming more prevalent in canine sbf cases. the increasi ... | 2014 | 24345778 |
prevalence of methicillin-resistant staphylococcus spp. in the conjunctival sac of healthy dogs. | to determine the prevalence of selected coagulase-positive methicillin-resistant staphylococcus aureus (mrs) in the conjunctival sac in a group of healthy dogs and to compare the prevalence of ocular mrs colonization with colonization of typically assessed body sites including the nasal cavity and rectum. | 2015 | 24299189 |
characterisation of a catalase-negative methicillin-resistant staphylococcus aureus isolate from a dog. | a catalase-negative mrsa strain and a methicillin resistant staphylococcus pseudintermedius strain (mrsp) were isolated from a dog affected by a severe form of pododermatitis. the catalase negative isolate was typed as sccmec i, pvl negative, st5 t002 strain. a deletion at position 487 of the kat gene altered the functionality of the catalase enzyme. this is the first report of a catalase-negative mrsa in animals. as catalase test is a rapid assay routinely employed for the identification of sta ... | 2013 | 24139722 |
evaluation of the affinity of various species and strains of staphylococcus to adhere to equine corneocytes. | meticillin-resistant staphylococcus aureus (mrsa) strain usa 500 predominately colonizes horses and people working with them. previous studies demonstrate that some staphylococcus species exhibit higher affinity for corneocytes of specific mammalian species. | 2013 | 23992283 |
first report in south america of companion animal colonization by the usa1100 clone of community-acquired meticillin-resistant staphylococcus aureus (st30) and by the european clone of methicillin-resistant staphylococcus pseudintermedius (st71). | methicillin-resistant staphylococci can colonize and cause diseases in companion animals. unfortunately, few molecular studies have been carried out in brazil and other countries with the aim of characterizing these isolates. consequently, little is known about the potential role of companion animals in transmitting these resistant bacteria to humans. in this work we searched for meca gene among staphylococcus isolates obtained from nasal microbiota of 130 healthy dogs and cats attended in a vet ... | 2013 | 23981343 |
animal and human staphylococcus aureus associated clonal lineages and high rate of staphylococcus pseudintermedius novel lineages in spanish kennel dogs: predominance of s. aureus st398. | methicillin-susceptible staphylococcus aureus (mssa) and staphylococcus pseudintermedius (mssp) are gaining interest to track the evolution of emerging methicillin-resistant strains in animals and humans. we focused on the characterization of the methicillin-susceptible coagulase-positive staphylococci (mscops) recovered from nasal samples of 98 healthy kennel-dogs. isolates were typed by spa, agr, mlst and smai/apai-pfge. antimicrobial resistance and virulence profiles were investigated. presen ... | 2013 | 23932077 |
clonal dynamics of nasal staphylococcus aureus and staphylococcus pseudintermedius in dog-owning household members. detection of mssa st(398). | the objective of this study was to investigate the dynamics of nasal carriage by staphylococcus aureus (sa) and staphylococcus pseudintermedius (sp) among healthy dog-owning household members involved in 7 previous index cases of suspected anthropozoonotic (n = 4) and zoonotic (n = 3) interspecies transmission [4 direct cases, identical sa (n = 3) or sp (n = 1) in owner and dog; three indirect, sp in owner (n = 2) or sa in dog (n = 1)]. co-carriage with methicillin-resistant coagulase-negative s ... | 2013 | 23874949 |
detection of mecc-positive staphylococcus aureus (cc130-mrsa-xi) in diseased european hedgehogs (erinaceus europaeus) in sweden. | recently, a novel mec gene conferring beta-lactam resistance in staphylococcus aureus has been discovered. this gene, mecc, is situated on a sccmec xi element that has to date been identified in clonal complexes 49, 130, 425, 599 and 1943. some of the currently known isolates have been identified from animals. this, and observations of meca alleles that do not confer beta-lactam resistance, indicate that mec genes might have a reservoir in staphylococcus species from animals. thus it is importan ... | 2013 | 23776626 |
first detection of methicillin-resistant staphylococcus aureus st398 and staphylococcus pseudintermedius st68 from hospitalized equines in spain. | eight coagulase-positive staphylococci from equines with different pathologies obtained between 2005 and 2011 were investigated. isolates were characterized by different molecular techniques (spa-, agr-, mlst), and clonal relatedness of strains was investigated by apai and smai pfge. anti-microbial resistance and virulence profiles were determined. six isolates were identified as staphylococcus aureus, and two as staphylococcus pseudintermedius. of these, four isolates were methicillin-resistant ... | 2014 | 23773775 |
public health impact and antimicrobial selection of meticillin-resistant staphylococci in animals. | whilst meticillin-resistant staphylococcus aureus (mrsa) infections reported sporadically in companion animals and cattle in the 1970s were probably of human origin, the recently emerged livestock-associated mrsa (la-mrsa) and meticillin-resistant staphylococcus pseudintermedius (mrsp) strains clearly have animal origins and their isolation from humans is usually associated with exposure to livestock and companion animals, respectively. la-mrsa is primarily an occupational health risk to farm wo ... | 2013 | 27873579 |
methicillin-resistant staphylococcal contamination of clothing worn by personnel in a veterinary teaching hospital. | to determine the methicillin-resistant staphylococcus aureus (mrsa) and methicillin-resistant staphylococcus pseudintermedius (mrsp) contamination rate of white coats (wc) and surgical scrubs (ss) worn by personnel at the ontario veterinary college health sciences centre (ovchsc) and to identify risk factors associated with clothing contamination. | 2013 | 23662728 |
a comparative evaluation of methicillin-resistant staphylococci isolated from harness racing-horses, breeding mares and riding-horses in italy. | to investigate the prevalence of methicillin-resistant staphylococci (mrs) which is a potencial risk factor of transmission between animals and humans in different types of horses (harness racing-horses, breeding mares and riding-horses) and to compare the antimicrobial resistance of the isolates. | 2013 | 23620832 |
efficacy of clarithromycin on biofilm formation of methicillin-resistant staphylococcus pseudintermedius. | surgical site infections (ssis) caused by biofilm-forming methicillin-resistant staphylococcus pseudintermedius (mrsp) have emerged as the most common hospital-acquired infections in companion animals. no methods currently exist for the therapeutic remediation of ssis caused by mrsp in biofilms. clarithromycin (cla) has been shown to prevent biofilm formation by staphylococcus aureus. this study aims to assess the in vitro activity of cla in eradicating mrsp biofilm formation on various material ... | 2012 | 23171620 |
high diversity of staphylococcus aureus and staphylococcus pseudintermedius lineages and toxigenic traits in healthy pet-owning household members. underestimating normal household contact? | forty-three unrelated pet-owning households were screened in spain to study the staphylococcus aureus and staphylococcus pseudintermedius nasal carriage, their genetic lineages and virulence traits. sixty-seven healthy owners and 66 healthy pets were investigated. isolates characterization was performed and potential interspecies transmission was assessed. s. aureus was present in 51.2% of households studied while s. pseudintermedius in 30.2%. twenty-eight owners (41.8%) carried s. aureus: one m ... | 2013 | 23153600 |
case-control study of staphylococcus lugdunensis infection isolates from small companion animals. | coagulase-negative staphylococcus lugdunensis has recently been shown to cause invasive infections of people, which are similar in pathogenic effect to those caused by staphylococcus aureus. little is known about the pathogenicity of s. lugdunensis in companion animals. | 2012 | 22862546 |
in vitro miconazole susceptibility of meticillin-resistant staphylococcus pseudintermedius and staphylococcus aureus. | the emergence and dissemination of meticillin-resistant staphylococci has created significant treatment challenges in veterinary medicine and increased interest in topical therapy for superficial infections. concern has been expressed regarding the use of some topical antimicrobials in animals because of the potential for emergence of resistance, and additional options are required. miconazole has limited antibacterial properties that include antistaphylococcal activity. | 2012 | 22830415 |
evaluation of cefoxitin disk diffusion breakpoint for detection of methicillin resistance in staphylococcus pseudintermedius isolates from dogs. | cefoxitin disk diffusion susceptibility testing is a recommended screening method for the detection of methicillin resistance in human isolates of staphylococcus aureus and coagulase-negative staphylococci. a retrospective analysis of 1,146 clinical isolates of staphylococcus pseudintermedius from dogs was conducted to determine if screening by the cefoxitin disk method can be similarly useful with s. pseudintermedius. the distribution of cefoxitin growth inhibition zone diameters within this co ... | 2012 | 22786971 |
occurrence of methicillin-resistant staphylococci in surgically treated dogs and the environment in a swedish animal hospital. | to investigate whether hospitalised dogs treated surgically may become culture positive for methicillin-resistant staphylococcus pseudintermedius or methicillin-resistant staphylococcus aureus. | 2012 | 22747733 |
evidence for production of a bacteriocin-like substance by staphylococcus pseudintermedius, inhibitory to staphylococcus aureus from foods. | this study assessed the production of a bacteriocin-like substance by staphylococcus pseudintermedius s28, and evaluates its inhibitory effect against isolates of s. aureus from foods. all indicator isolates were sensitive to the substance produced from s. pseudintermedius s28, showing growth inhibition zones ranging from 14.2 to 28.3 mm. the inhibitory substance has no effect against the producer strain. the inhibitory substance was affected by proteolytic enzymes, while glycolytic and lipolyti ... | 2013 | 22703567 |
methicillin-resistant staphylococcal contamination of cellular phones of personnel in a veterinary teaching hospital. | hospital-associated infections are an increasing cause of morbidity and mortality in veterinary patients. with the emergence of multi-drug resistant bacteria, these infections can be particularly difficult to eradicate. sources of hospital-associated infections can include the patients own flora, medical staff and inanimate hospital objects. cellular phones are becoming an invaluable feature of communication within hospitals, and since they are frequently handled by healthcare personnel, there m ... | 2012 | 22533923 |
genetic and phenotypic characterization of methicillin-resistant staphylococci isolated from veterinary hospitals in south korea. | staphylococci were isolated from veterinary staff, hospitalized animals, and medical equipment from 2 major tertiary veterinary hospitals in south korea to investigate antimicrobial resistance and genetic relatedness. the detection rate for staphylococci was 55.2% (111/201 samples), and 11 species were identified among the collected staphylococcal strains. the most prevalent species were staphylococcus pseudintermedius (52/111, 46.8%), staphylococcus epidermidis (21/111, 18.9%), and staphylococc ... | 2012 | 22529115 |
staphylococcus pseudintermedius in the dog: taxonomy, diagnostics, ecology, epidemiology and pathogenicity. | the dog is the natural host of staphylococcus pseudintermedius. many research efforts are currently being undertaken to expand our knowledge and understanding of this important canine commensal and opportunistic pathogen. the objective of this review is to summarize the current knowledge of the species, including the latest research outcomes, with emphasis on taxonomy, diagnostics, ecology, epidemiology and pathogenicity. despite the important taxonomic changes that have occurred over the past f ... | 2012 | 22515504 |
recovery of staphylococci from computer keyboards in a veterinary medical centre and the effect of routine cleaning. | computers play a vital role in veterinary clinics for grading, examining results, updating records, giving discharge instructions and maintaining billing information. few studies have documented the degree of contamination or practical methods to disinfect computer equipment within the veterinary clinic setting. the intent of the present study was to characterise the frequency of recovery of staphylococcus species from computer keyboards from a veterinary teaching hospital setting and to evaluat ... | 2012 | 22447457 |
in vitro antimicrobial activity of miconazole and polymyxin b against canine meticillin-resistant staphylococcus aureus and meticillin-resistant staphylococcus pseudintermedius isolates. | meticillin-resistant staphylococcus aureus (mrsa) and meticillin-resistant staphylococcus pseudintermedius (mrsp) infections are increasingly reported in dogs, and these bacteria may be isolated from ear infections. | 2012 | 22409321 |
prevalence of meticillin-resistant staphylococcus pseudintermedius (mrsp) from skin and carriage sites of dogs after treatment of their meticillin-resistant or meticillin-sensitive staphylococcal pyoderma. | meticillin-resistant staphylococci are significant pathogens in veterinary dermatology, yet longitudinal studies of the impact of routine antimicrobial therapy on emergence or resolution of resistance are lacking. | 2012 | 22364707 |
selection of antibiotics for meticillin-resistant staphylococcus pseudintermedius: time to revisit some old drugs? | the aim of this review is to consider systemic therapy options for meticillin-resistant staphylococcus pseudintermedius (mrsp). infections caused by mrsp in small animals--particularly dogs--have been frustrating veterinarians in recent years. after a susceptibility test is performed, veterinarians are left to select from drugs that have not been frequently encountered on a susceptibility report. some of these are old drugs that have not been used regularly by veterinary dermatologists. as mrsp ... | 2012 | 22313056 |
comparative activity of pradofloxacin and marbofloxacin against coagulase-positive staphylococci in a pharmacokinetic-pharmacodynamic model based on canine pharmacokinetics. | pradofloxacin (pra), a novel veterinary 8-cyano-fluoroquinolone (fq), is active against staphylococcus pseudintermedius, the primary cause of canine pyoderma. an in vitro pharmacokinetic-pharmacodynamic model was used to compare the activities of pra and marbofloxacin (mar) against three clinical isolates of s. pseudintermedius and reference strain staphylococcus aureus atcc 6538. experiments were performed involving populations of 10(10) cfu corresponding to an inoculum density of approximately ... | 2012 | 22276681 |