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life cycle of gnathostoma nipponicum yamaguti, 1941.the life cycle of gnathostoma nipponicum was examined by field survey and by experimental infection of animals with the larvae. naturally infected larval g. nipponicum were found in loaches, catfish, and snakes. experimentally, loaches, killifishes, frogs, salamanders, mice, and rats were successfully infected with the early third-stage larvae of g. nipponicum obtained from copepods (the first intermediate host), whereas snakes, quails, and weasels were not. frogs, snakes, quails, and rats were ...19921469260
five confirmed human cases of gnathostomiasis nipponica recently found in northern japan.five confirmed human cases of gnathostomiasis nipponica exhibiting creeping eruption and itching were found sporadically from the autumn of 1991 to the winter of 1992 in the northern region of the mainland of japan. in all cases, a causative gnathostome with 3 transverse rows of hooklets on the head bulb was detected in biopsied skin. the morphological characteristics agreed with the advanced third-stage larvae of gnathostoma nipponicum. within a few weeks before development of symptoms, all pat ...19921491291
human gnathostomiasis.two patients became infested with gnathostoma nipponicum after eating raw loach-fish they had caught in a rice field in central japan. a fragment of gnathostoma was found in a biopsy from one of them. the sera of both patients reacted with gnathostoma antigen using indirect immunofluorescence. scanning electron microscopy was performed on blocks of the paraffin-embedded parasite sample and the viscera of a fish from the same rice field. the risk of eating raw freshwater fish is discussed.19911856338
two human cases of gnathostomiasis and discovery of a second intermediate host of gnathostoma nipponicum in japan.two human cases of gnathostomiasis from ingestion of raw native japanese loaches, misgurnus anguillicaudatus, are reported. seven early third-stage larval gnathostoma nipponicum were recovered from 3,098 loaches in the same district in which 2 human patients had obtained and eaten raw loaches. encapsulated g. nipponicum larvae were also recovered from loaches infected under laboratory conditions. all 6 weasels captured in the same district in which the naturally infected loaches were found and w ...19883397824
migration and development of the larvae of gnathostoma nipponicum in the rat, second intermediate or paratenic host, and the weasel, definitive host.migration and development of the larvae of gnathostoma nipponicum in the rat as second intermediate or paratenic host and the weasel as definitive host were examined to clarify the pathogenesis in each animal. the advanced third-stage larvae (adl3) given orally to rats remained in the stomach for 12 h post-infection (pi). after that, larvae transiently invaded various organs and finally moved into the muscle within 48 h. the percentage of eosinophils in the blood taken from the jugular vein of r ...19948006379
larval gnathostoma nipponicum found in the imported chinese loaches.six early third-stage larvae of gnathostoma nipponicum were recovered from the muscle of 376 loaches, misgurnus anguillicaudatus, imported from china. they were 614 x 114 microns in average size, almost colorless except brownish intestine, and encircled by about 229 transverse rows of minute cuticular spines. their head bulbs provided with 3 rows of hooklets, of which average number were 34.5 on the first, 36.7 on the second and 39.7 on the third. accordingly, it is revealed that the chinese loa ...19938297892
discovery of mammalian hosts to gnathostoma nipponicum larvae and prevalence of the larvae in rodents and insectivores.from september 1993 to november 1995, to clarify the natural infection of gnathostoma nipponicum (g.n.) larvae in small wild mammals, a total of 313 animals consisted of three species of rodents and two species of insectivores, collected from an endemic area of this nematode in aomori prefecture, were investigated. two species, rattus norvegicus (r.n.) and chimarrogale himalayica (c.h.) were infected with g.n. larvae, and the infection rate was extremely high with 27.2% and 72.7% in the former a ...19968898280
survey for gnathostoma nipponicum larvae in gobiid freshwater fish and infectivity of the larvae to a gobiid fish (chaenogobius urotaenia).from june 1993 to november 1996, a total of 977 gobiid fish consisting of three species (tridentiger brevispinis, chaenogobius urotaenia, and rhinogobiu brunneus) collected from eastern aomori prefecture, were examined for gnathostoma nipponicum larvae infection. only one species, c. urotaenia was infected with advanced third-stage larvae (adl3), and a total of 22 larvae were recovered from 17 (3.4%) of 500 fish. the infected fish were larger than 12 cm in body length and collected in the may-ju ...19979300363
susceptibility of several species of cyprinidae and salmonidae freshwater fish to larval gnathostoma nipponicum infection.susceptibility of five species of cyprinidae and salmonidae freshwater fish to the early third-stage larvae (eal3) and advanced third-stage larvae (adl3) of gnathostoma nipponicum infection were examined. two fish species inoculated orally with eal3 were infected, and adl3 were recovered from them with rate of 21.0% in tribolodon hakonensis and 0.5% in cyprinus carpio at 30 days postinoculation (pi). attempts to infect five fish species with adl3 were all successful. the recovery rate of adl3 wa ...19979409521
spontaneous larval gnathostoma nipponicum infection in frogs.from june 1993 to september 1997, a survey was carried out for the prevalence of larval gnathostoma nipponicum infection in several kinds of frogs, toads, and their tadpoles collected from an endemic area of this nematode in aomori prefecture. two frog species, one of 436 (0.2%) rana nigromaculata and 51 of 147 (34.7%) r. catesbeiana were infected, and a total of 446 advanced third-stage larvae (adl3) of g. nipponicum were recovered. these results confirmed that two frog species which can serve ...19989795906
characterization of monoclonal antibodies against gnathostoma nipponicum.monoclonal antibodies (mabs) were produced against the proteins of advanced third-stage larvae (adl3) of gnathostoma nipponicum. six mabs (gn2c3, gn2h3, gn4c3, gn4e9, gnsh1, and gn10b7) were obtained as determined by enzyme-linked immunosorbent assay (elisa). gn4e9 and gnsh1 seemed to be genus-specific, as they did not cross-react with anisakis sp., dirofilaria immitis, gongylonema pulchrum, toxocara canis, trichinella sp., trichuris vulpis, metagonimus sp., or spirometra erinaceieuropaei by eli ...200312659325
a human case of gnathostomiasis nipponica confirmed indirectly by finding infective larvae in leftover largemouth bass meat.a human case of creeping eruption due to gnathostoma nipponicum was confirmed indirectly by finding infective advanced third-stage larvae in leftover largemouth bass meat. this is the first report indicating that the largemouth bass (micropterus salmoides) serves as a source of g. nipponicum infection in humans.200312760668
[reports of gnathostoma nipponicum yamaguti, 1941, in kumamoto-ken]. 196514340657
surface ultrastructure of the advanced third-stage larvae of gnathostoma nipponicum.the surface ultrastructure of advanced third-stage larvae (al3) of gnathostoma nipponicum was studied using scanning electron microscopy. the larvae were recovered from the grass snake rhabdophis tigrina in the republic of korea. parasites had a globular head bulb with a pair of lips at the anterior end and 2 labial papillae and an amphid on each lip. the head bulb was characteristically armed with 3 transverse rows of hooklets, averaging 36, 38, and 43 in number, increasing posteriorly. a total ...200314740920
the jeju weasel, mustela sibilica quelpartis, a new definitive host for gnathostoma nipponicum yamaguti, 1941.adult gnathostomes were discovered in the stomach of the jeju weasel, mustela sibilica quelpartis, road-killed in jeju-do (province). their morphological characters were examined to identify the species. total 50 gnathostome adults were collected from 6 out of 10 weasels examined. in infected weasels, 4-6 worms were grouped and embedded in each granulomatous gastric tumor, except 1 weasel. male worms were 25.0×1.4 mm in average size, and had a tail with pedunculate papillae, a spicule, and minut ...201122072837
discovery of larval gnathostoma nipponicum in frogs and snakes from jeju-do (province), republic of korea.a survey was performed to find out the intermediate hosts of gnathostoma nipponicum in jeju-do (province), the republic of korea. in august 2009 and 2010, a total of 82 tadpoles, 23 black-spotted pond frogs (rana nigromaculata), 7 tiger keelback snakes (rhabdophis tigrinus tigrinus), 6 red-tongue viper snakes (agkistrodon ussuriensis), and 2 cat snakes (elaphe dione) were collected in jeju-do and examined by the pepsin-hcl digestion method. total 5 gnathostome larvae were detected in 3 (50%) of ...201122355217
complete mitochondrial genomes of gnathostoma nipponicum and gnathostoma sp., and their comparison with other gnathostoma species.gnathostomiasis is a foodborne zoonotic parasitosis caused by gnathostoma nematodes. it has caused significant public problems worldwide, but its molecular biology is limited. the purpose of this study was to decode the complete mitochondrial (mt) genomes of gnathostoma nipponicum and gnathostoma sp., and compare their mt sequences with other gnathostoma species. the complete mt genome sequences were amplified by long-range pcr and determined by subsequent primer walking. the complete mt genomes ...201728025097
imported intraocular gnathostomiasis with subretinal tracks confirmed by western blot assay.we report a case of intraocular gnathostomiasis diagnosed by western blot assay in a patient with subretinal tracks. a 15-year-old male patient complained of blurred vision in the right eye, lasting for 2 weeks. eight months earlier, he had traveled to vietnam for 1 week and ate raw wild boar meat and lobster. his best-corrected visual acuity was 20/20 in both eyes and anterior chamber examination revealed no abnormalities. fundus examination showed subretinal tracks in the right eye. fluorescei ...201222451738
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