| complete genomic dna sequence of rock bream iridovirus. | iridovirus is a causative agent of epizootics among cultured rock bream (oplegnathus fasciatus) in korea. here, we report the complete genomic sequence of rock bream iridovirus (rbiv). the genome of rbiv was 112080 bp long and contained at least 118 putative open reading frames (orfs), and its genome organization was similar to that of infectious spleen and kidney necrosis virus (isknv). of the rbiv's 118 orfs, 85 orfs showed 60-99% amino acid identity to those of isknv. phylogenetic analysis of ... | 2004 | 15246274 |
| complete genome sequence analysis of an iridovirus isolated from the orange-spotted grouper, epinephelus coioides. | orange-spotted grouper iridovirus (osgiv) was the causative agent of serious systemic diseases with high mortality in the cultured orange-spotted grouper, epinephelus coioides. here we report the complete genome sequence of osgiv. the osgiv genome consists of 112,636 bp with a g+c content of 54%. 121 putative open reading frames (orf) were identified with coding capacities for polypeptides varying from 40 to 1168 amino acids. the majority of osgiv shared homologies to other iridovirus genes. phy ... | 2005 | 15964605 |
| phylogenetic analysis of the major capsid protein gene of iridovirus isolates from cultured flounders paralichthys olivaceus in korea. | in 2003, 13 isolates of iridovirus were obtained from cultured flounders paralichthys olivaceus during epizootics in korea. the full open reading frames (orfs) encoding the major capsid protein (mcp) (1362 bp) from the 13 flounder iridoviruses (flivs) were sequenced and the deduced amino acid sequences were phylogenetically analyzed. phylogenetic analysis of the mcp revealed that all 13 flivs were the same species as rock bream iridovirus (rbiv), red sea bream iridovirus (rsiv), and infectious s ... | 2005 | 15997817 |
| molecular epidemiology and phylogenetic analysis of a marine fish infectious spleen and kidney necrosis virus-like (isknv-like) virus. | infectious spleen and kidney necrosis virus-like (isknv-like) virus causes a serious systemic disease with high morbidity and mortality of freshwater and marine fishes. based on the isknv putative major capsid protein (mcp), the vascular endothelial growth factor (vegf), the mrna capping enzyme (capping), and the tumor necrosis factor receptor-associated protein (tnfr) genes, primers were designed and used in pcr to determine the host range of isknv-like viruses. from the sampling of >1600 marin ... | 2007 | 17131065 |
| comparative genomic analysis of the family iridoviridae: re-annotating and defining the core set of iridovirus genes. | members of the family iridoviridae can cause severe diseases resulting in significant economic and environmental losses. very little is known about how iridoviruses cause disease in their host. in the present study, we describe the re-analysis of the iridoviridae family of complex dna viruses using a variety of comparative genomic tools to yield a greater consensus among the annotated sequences of its members. | 2007 | 17239238 |
| expression and serological application of a capsid protein of an iridovirus isolated from rock bream, oplegnathus fasciatus (temminck & schlegel). | iridoviruses infect a wide variety of wild and cultured fish. those iridoviruses belonging to the genus ranavirus, in the iridoviridae family, cause systemic disease in infected animals with a high morbidity and mortality. this paper reports the cloning, sequencing, and expression of the rock bream iridovirus (rbiv) major capsid protein (mcp) in an escherichia coli expression system for subsequent immunological studies. the completeness of the expressed protein was confirmed by peptide mass fing ... | 2007 | 17958613 |
| functional characterization of the rnase iii gene of rock bream iridovirus. | all of the fully sequenced iridoviruses have an orf resembling a putative rnase iii gene. however, to the best of our knowledge, functional characterization of the iridovirus-encoded rnase iii has not been done. in the present study, we have characterized the putative rnase iii of rock bream iridovirus (rbiv), the major cause of mass mortality of cultured rock bream oplegnathus fasciatus in korea. rbiv rnase iii has a single n-terminal endonuclease domain followed by a c-terminal double-stranded ... | 2008 | 18641914 |
| production of polyclonal antibody against recombinant orf 112 l of rock bream (oplegnathus fasciatus) iridovirus (rbiv) and in vitro neutralization. | rock bream iridovirus (rbiv) is a causative agent of epizootics among cultured rock bream (oplegnathus fasciatus) in korea. in this study, the immunogenic property of ankyrin repeats gene (orf 112l) from rbiv was evaluated to develop vaccines against rbiv. orf 112l of rbiv was cloned into expression vector of pgex-4t-1. the recombinant protein was successfully expressed using e. coli bl21 (de3). the soluble recombinant rbiv protein was applied to affinity column for the purification of the prote ... | 2008 | 19195399 |
| development of sirna expression vector utilizing rock bream beta-actin promoter: a potential therapeutic tool against viral infection in fish. | in the present study, we have developed short interfering rna (sirna) expression vector utilizing rock bream beta-actin promoter and examined the possible use for the inhibition of highly pathogenic fish virus, rock bream iridovirus (rbiv), replication in vitro. initially, in order to express sirna effectively, we added several modifications to wild-type rock bream beta-actin promoter. next, we succeeded in knocking down the expression of enhanced green fluorescent protein reporter gene expressi ... | 2010 | 19685238 |
| identification of the vp92r gene from infectious spleen and kidney necrosis virus. | megalocytiviruses, which belong to the family iridoviridae, are among the most harmful viruses to cultured fishes. infectious spleen and kidney necrosis virus (isknv) is the type species of the megalocytivirus genus. the genome of isknv was sequenced and annotated by he et al. (virology 291:126-139, 2001) in 2001 and re-annotated by eaton et al. (virol. j. 4:11, 2007) in 2007. the orf092r of isknv was identified in the annotations of he et al. but was not reported by eaton et al. in this study, ... | 2010 | 20577793 |
| complete genome sequence of a megalocytivirus (family iridoviridae) associated with turbot mortality in china. | turbot reddish body iridovirus (trbiv) causes serious systemic diseases with high mortality in the cultured turbot, scophthalmus maximus. we here sequenced and analyzed the complete genome of trbiv, which was identified in shandong province, china. | 2010 | 20630106 |
| heparin cofactor ii (rbhcii) from rock bream (oplegnathus fasciatus): molecular characterization, cloning and expression analysis. | heparin cofactor (hcii) is a serine protease inhibitor (spi), and plays important physiological roles in various biological events including hemostasis. the gene encoding the hcii was isolated from gs-flx™ genomic data of rock bream (oplegnathus fasciatus), designated as rbhcii. the rbhcii (1950 bp) consists of a 1512 bp open reading frame (orf) encoding 504 amino acids (aa), with a signal peptide of 19 aa residues. the predicted molecular mass and the estimated isoelectric point of rbhcii were ... | 2010 | 20951808 |
| characterization and expression analysis of a goose-type lysozyme from the rock bream oplegnathus fasciatus, and antimicrobial activity of its recombinant protein. | lysozyme (muramidase) represents an important defense molecule of the fish innate immune system. known for its bactericidal properties, lysozyme catalyzes the hydrolysis of β-(1,4)-glycosidic bonds between the n-acetyl glucosamine and n-acetyl muramic acid in the peptidoglycan layer of bacterial cell walls. in this study, the complete coding sequence of a g-type lysozyme (rbglyz) was identified in the oplegnathus fasciatus rock bream fish genome by means of multi-tissue normalized cdna pyroseque ... | 2010 | 21167286 |
| megalocytiviruses. | the genus megalocytivirus, represented by red sea bream iridovirus (rsiv), the first identified and one of the best characterized megalocytiviruses, infectious spleen and kidney necrosis virus (isknv), the type species of the genus, and numerous other isolates, is the newest genus within the family iridoviridae. viruses within this genus are causative agents of severe disease accompanied by high mortality in multiple species of marine and freshwater fish. to date outbreaks of megalocytivirus-ind ... | 2012 | 22590684 |
| thioredoxin domain-containing protein 12 from oplegnathus fasciatus: molecular characterization, expression against immune stimuli, and biological activities related to oxidative stress. | thioredoxin domain-containing protein 12 (txndc12) is a small, disulfide-containing protein that belongs to the thioredoxin (txn) superfamily. in the present study, we identified and characterized a txndc12-like gene, designated oftxndc12, from rock bream, oplegnathus fasciatus. oftxndc12 consists of seven exons interrupted by six introns. comparative genomic structural analysis revealed that the txndc12 of vertebrates is a structurally conserved gene. the coding sequence of oftxndc12 comprises ... | 2016 | 27026037 |
| molecular, genomic, and expressional delineation of a piscidin from rock bream (oplegnathus fasciatus) with evidence for the potent antimicrobial activities of of-pis1 peptide. | the piscidin family comprises a group of antimicrobial peptides (amps) that are vital components of teleost innate immunity. piscidins protect the host from pathogens, through multifaceted roles as immunomodulators and anti-infective peptides. the present study reports the identification, and characterization of a putative piscidin homolog, of-pis1, from rock bream (oplegnathus fasciatus). a combined genomic and transcriptomic approach revealed that the of-pis1 gene comprises 1396 nucleotides (n ... | 2016 | 26549174 |
| gene structure, molecular characterization and transcriptional expression of two p38 isoforms (mapk11 and mapk14) from rock bream (oplegnathus fasciatus). | the p38 kinases are one of the four subgroups of mitogen-activated protein kinase (mapk) superfamily which are involved in the innate immunity. the p38 subfamily that includes four members namely p38α (mapk14), p38β (mapk11), p38γ (mapk12) and p38δ (mapk13), regulates the activation of several transcription factors. in this study, a p38β (ofmapk11) homolog and a p38α (ofmapk14) homolog of oplegnathus fasciatus were identified at genomic level. results clearly showed that both mapk11 and mapk14 a ... | 2015 | 26363230 |
| characterization of rock bream (oplegnathus fasciatus) complement components c1r and c1s in terms of molecular aspects, genomic modulation, and immune responsive transcriptional profiles following bacterial and viral pathogen exposure. | the complement components c1r and c1s play a crucial role in innate immunity via activation of the classical complement cascade system. as initiators of the pathogen-induced signaling cascade, c1r and c1s modulate innate immunity. in order to understand the immune responses of teleost c1r and c1s, oplegnathus fasciatus c1r and c1s genes (ofc1r and ofc1s) were identified and characterized. the genomic sequence of ofc1r was enclosed with thirteen exons that represented a putative peptide with 704 ... | 2015 | 26241508 |
| insights into molecular profiles and genomic evolution of an irak4 homolog from rock bream (oplegnathus fasciatus): immunogen- and pathogen-induced transcriptional expression. | as a pivotal signaling mediator of toll-like receptor (tlr) and interleukin (il)-1 receptor (il-1r) signaling cascades, the il-1r-associated kinase 4 (irak4) is engaged in the activation of host immunity. this study investigates the molecular and expressional profiles of an irak4-like homolog from oplegnathus fasciatus (ofirak4). the ofirak4 gene (8.2 kb) was structured with eleven exons and ten introns. a putative coding sequence (1395bp) was translated to the ofirak protein of 464 amino acids. ... | 2015 | 25555811 |
| mitochondrial peroxiredoxin 3 (prx3) from rock bream (oplegnathus fasciatus): immune responses and role of recombinant prx3 in protecting cells from hydrogen peroxide induced oxidative stress. | pathogenic infections and environmental factors cause a variety of stresses in fish including oxidative stress by rapid elevation of reactive oxygen species (ros) and reactive nitrogen species (rns). transcriptional activation and expression of antioxidant enzymes are essential for reducing the oxidative stress. in this study, we present the molecular characterization, immune responses and ros scavenging activity of mitochondrial peroxiredoxin 3 from oplegnathus fasciatus (rbprx3). coding sequen ... | 2015 | 25542382 |
| two carboxypeptidase counterparts from rock bream (oplegnathus fasciatus): molecular characterization, genomic arrangement and immune responses upon pathogenic stresses. | carboxypeptidases (cps) are proteases that hydrolyze c-terminal peptide bonds. they are involved in regulating the complement system of the immune system. here, we report the molecular characterization and immune response of two carboxypeptidases, named carboxypeptidase a (rb-cpa) and carboxypeptidase n1 (rb-cpn1), from rock bream. the genomic sequence of rb-cpa contains 12 exons interrupted by 11 introns, while the genomic sequence of rb-cpn1 has 9 exons and 8 introns. the cdna sequence of rb-c ... | 2014 | 25457548 |
| molecular genomic- and transcriptional-aspects of a teleost traf6 homolog: possible involvement in immune responses of oplegnathus fasciatus against pathogens. | tumor necrosis factor receptor (tnfr)-associated factor 6 (traf6) is a crucial docking molecule for tnfr superfamily and interleukin-1 receptor/toll-like receptor (il-1r/tlr) superfamily. as an adaptor protein in pathogen-induced signaling cascades, traf6 modulates both adaptive- and innate-immunity. in order to understand the immune responses of teleost traf6, oplegnathus fasciatus traf6-like gene (oftraf6) was identified and characterized. genomic length of oftraf6 (4 kb), obtained by means of ... | 2015 | 25449707 |
| molecular characterization and transcriptional analysis of non-mammalian type toll like receptor (tlr21) from rock bream (oplegnathus fasciatus). | toll-like receptors (tlrs) are a large family of pattern recognition receptors, which are involved in triggering host immune responses against various pathogens by detecting their evolutionarily conserved pathogen associated molecular patterns (pamps). tlr21 is a non-mammalian type tlr, which recognizes unmethylated cpg dna, and is considered as a functional homolog of mammalian tlr9. in this study, we attempted to identify and characterize a novel tlr21 counterpart from rock bream (oplegnathus ... | 2014 | 25300254 |
| genomic structure and immunological response of an stat4 family member from rock bream (oplegnathus fasciatus). | the janus tyrosine kinase (jak)/signal transducer and activator of transcription (stat) signaling pathway plays a critical role in host defense against viral and bacterial infections. stat proteins are a group of transcription factors that translocate into the nucleus and are critical for the induction of many genes crucial for the allergic cascade and immune defense. in the present study, a member of the stat4 family was identified from rock bream (rbstat4) at the genomic level, and its transcr ... | 2013 | 24055510 |
| three complement component 1q genes from rock bream, oplegnathus fasciatus: genome characterization and potential role in immune response against bacterial and viral infections. | complement component 1q (c1q) is a subcomponent of the c1 complex and the key protein that recognizes and binds to a broad range of immune and non-immune ligands to initiate the classical complement pathway. in the present study, we identified and characterized three novel c1q family members from rock bream, oplegnathus fasciatus. the full-length cdnas of c1q a-like (rbc1qal), c1q b-like (rbc1qbl), and c1q c-like (rbc1qcl) consist of 780, 720 and 726 bp of nucleotide sequence encoding polypeptid ... | 2013 | 23994081 |
| marine teleost ortholog of catalase from rock bream (oplegnathus fasciatus): molecular perspectives from genomic organization to enzymatic behavior with respect to its potent antioxidant properties. | catalases are well known antioxidant enzymes that can mainly dismutate hydrogen peroxide into water and oxygen in order to prevent oxidative stress. the complete genomic dna (gdna) sequence of the catalase gene from rock bream (oplegnathus fasciatus) was identified from our custom-constructed bac genomic dna library and designated as rbcat. rbcat consists of 13 exons, separated by 12 introns, within a 13,722-bp gdna sequence. the complete cdna sequence (3303 bp) of rbcat is comprised of a 1581-b ... | 2013 | 23872475 |
| genomic characterization and transcriptional evidence for the involvement of complement component 7 in immune response of rock bream (oplegnathus fasciatus). | the complement component 7 (c7) is the central mediator of pathogenic attack at the membrane surface and its binding to the c5b-7 complex triggers cytolytic signaling. in this study, c7 of rock bream (oplegnathus fasciatus) was identified (rb-c7) and characterized at the genomic level. the rb-c7 gene contains 18 exons and 17 introns and is composed of a 2490 bp complete open reading frame (orf). the encoded polypeptide (830 amino acids) contains a number of well-conserved c7 signature domains. i ... | 2013 | 23603298 |
| genomic characterization and expression analysis of complement component 8α and 8β in rock bream (oplegnathus fasciatus). | the complement component 8α and 8β are glycoproteins that mediate formation of the membrane attack complex (mac) on the surface of target cells. full-length complement c8α (rb-c8α) and c8β (rb-c8β) sequences were identified from a cdna library of rock bream (oplegnathus fasciatus), and their genomic sequences were obtained by screening and sequencing of a bacterial artificial chromosome (bac) genomic dna library of rock bream. the rb-c8α gene contains 64bp of 5'-utr, open reading frame (orf) of ... | 2013 | 23059376 |
| interferon regulatory factors 4 and 8 in rock bream, oplegnathus fasciatus: structural and expressional evidence for their antimicrobial role in teleosts. | the interferon regulatory factor (irf) members irf4 and irf8 contribute to b-lymphocyte development and can act as regulators of immunoglobulin (ig) light chain gene transcription. these two irfs are closely interrelated and are expressed at high levels in the lymphoid and myeloid cells of the immune system. in this study, the complete cdna and genomic sequences of rock bream irf4 (rbirf4) and irf8 (rbirf8) were identified by homology screening of a multi-tissue normalized cdna library and a bac ... | 2012 | 22885028 |
| genomic characterization and expression analysis of complement component 9 in rock bream (oplegnathus fasciatus). | the complement component 9 (c9) is a single-chain glycoprotein that mediates formation of the membrane attack complex (mac) on the surface of target cells. full-length c9 sequence was identified from a cdna library of rock bream (oplegnathus fasciatus), and its genomic sequence was obtained by screening and sequencing of a bacterial artificial chromosome (bac) genomic dna library of rock bream. the rock bream complement component 9 (rb-c9) gene contains 11 exons and 10 introns and is composed of ... | 2012 | 22796422 |
| caspase 3 from rock bream (oplegnathus fasciatus): genomic characterization and transcriptional profiling upon bacterial and viral inductions. | caspase 3 is a prominent mediator of apoptosis and participates in the cell death signaling cascade. in this study, caspase 3 was identified (rbcasp3) and characterized from rock bream (oplegnathus fasciatus). the full-length cdna of rbcasp3 is 2683 bp and contains an open reading frame of 849 bp, which encodes a 283 amino acid protein with a calculated molecular mass of 31.2 kda and isoelectric point of 6.31. the amino acid sequence resembles the conventional caspase 3 domain architecture, incl ... | 2012 | 22554851 |
| a novel acute phase reactant, serum amyloid a-like 1, from oplegnathus fasciatus: genomic and molecular characterization and transcriptional expression analysis. | acute phase response is a significant component of innate immunity, playing a vital role in the signaling processes and elimination of invading pathogens. acute phase proteins are synthesized in liver and secreted into the blood for transportation to an infection site, where the defense function is exerted. serum amyloid a (saa) and c-reactive proteins are the major positive acute phase proteins. in this study, we have identified and characterized a novel saa related gene from rock bream (oplegn ... | 2012 | 22504166 |
| a teleostean angiotensinogen from oplegnathus fasciatus responses to immune and injury challenges. | angiotensinogen (agt) is the precursor of the renin-angiotensin system and contributes to osmoregulation, acute-phase and immune responses. a full-length cdna of the agt (2004 bp with a 1389 bp coding region) was isolated from rock bream (rb), oplegnathus fasciatus. the encoded polypeptide of 463 amino acids had a predicted molecular mass of 51.6 kda. rbagt possessed a deduced signal peptide of 22 residues upstream of a putative angiotensin i sequence ((23)nrvyvhpfhl(32)). rbagt possessed a spec ... | 2012 | 22300787 |
| complete genome sequence and transcription profiles of the rock bream iridovirus rbiv-c1. | the family iridoviridae consists of 5 genera of double-stranded dna viruses, including the genus megalocytivirus, which contains species that are important fish pathogens. in a previous study, we isolated the first rock bream iridovirus from china (rbiv-c1) and identified it as a member of the genus megalocytivirus. in this report, we determined the complete genomic sequence of rbiv-c1 and examined its in vivo expression profiles. the genome of rbiv-c1 is 112333 bp in length, with a gc content o ... | 2013 | 23759558 |
| suppression of rnai by dsrna-degrading rnaseiii enzymes of viruses in animals and plants. | certain rna and dna viruses that infect plants, insects, fish or poikilothermic animals encode class 1 rnaseiii endoribonuclease-like proteins. dsrna-specific endoribonuclease activity of the rnaseiii of rock bream iridovirus infecting fish and sweet potato chlorotic stunt crinivirus (spcsv) infecting plants has been shown. suppression of the host antiviral rna interference (rnai) pathway has been documented with the rnaseiii of spcsv and heliothis virescens ascovirus infecting insects. suppress ... | 2015 | 25747942 |
| expression analysis of lily type lectin isotypes in the rock bream, oplegnathus fasciatus: in the tissue, developmental stage and viral infection. | lectins belong to the pattern-recognition receptors (prrs) class and play important roles in the recognition and elimination of pathogens via the innate immune system. recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks in the early immune response of fish. however, this study is limited to information that the lectin is involved in the innate immune response against viral infection. in the present study, the lily-type lectin-2 and -3 of oplegnathus fasciatus (o ... | 2016 | 28144635 |
| complement factor d homolog involved in the alternative complement pathway of rock bream (oplegnathus fasciatus): molecular and functional characterization and immune responsive mrna expression analysis. | the complement system serves conventional role in the innate defense against common invading pathogens. complement factor d (cfd) is vital to alternative complement pathway activation in cleaving complement factor b. this catalytic reaction forms the alternative c3 convertase that is crucial for complement-mediated pathogenesis. in this study, rock bream (oplegnathus fasciatus) cfd (ofcfd) was characterized and ofcfd mrna expression was investigated. ofcfd encodes 277 amino acids (aa) for a 30-k ... | 2016 | 27311435 |
| low pathogenicity of flounder iridovirus (fliv) and the absence of cross-protection between fliv and rock bream iridovirus. | the genus megalocytivirus is known to infect a wide range of cultured marine fish. in this study, we examined the pathogenicity of fliv (megalocytivirus from olive flounder, genotype iii) and rbiv (megalocytivirus from rock bream, genotype i) to their homologous and heterologous host species. olive flounder (7.5 ± 1.3 cm) injected with fliv [major capsid protein (mcp) gene copies, 6.8 × 10(3) -6.5 × 10(6) /fish] at 24 °c did not die until 90 days post-infection (dpi). the average virus replicati ... | 2016 | 27009694 |
| effects of water temperature on mortality in megalocytivirus-infected rock bream oplegnathus fasciatus (temminck et schlegel) and development of protective immunity. | rock bream iridovirus (rbiv) causes huge losses, especially in rock bream oplegnathus fasciatus. rock bream injected with rbiv and held at 29, 26, 23 or 20 °c had 100% mortality. conversely, all infected fish held at 17 °c survived even after the temperature was progressively increased to 26 °c at 100 dpi. rock bream exposed to virus and held for 2, 4 and 7 days at 23/26 °c before the temperature was reduced to 17 °c had mortality rates of 26.6/73.2%, 66.6/100% and 93.4/100%, respectively, throu ... | 2015 | 25073547 |
| gene expression of pro- and anti-apoptotic proteins in rock bream (oplegnathus fasciatus) infected with megalocytivirus (family iridoviridae). | viruses belonging to the genus megalocytivirus cause diseases in marine fishes primarily in east and southeast asian countries. rock bream iridovirus (rbiv), which is a member of the megalocytivirus genus, causes severe mass mortalities in rock beam (oplegnathus fasciatus) in korea. in this study, we assessed apoptosis-related gene expression patterns in megalocytivirus-infected rock bream in high mortality and low mortality conditions to determine important apoptosis-related factors, which may ... | 2014 | 24463468 |
| oral immunization of fish against iridovirus infection using recombinant antigen produced from rice callus. | fish iridoviruses cause systemic diseases with high morbidity and mortality in various species of wild and farm-raised fish, resulting in severe economic losses, and no large-scale protective vaccine program or therapy is currently available. in this study, we expressed a recombinant major capsid protein (rmcp) of rock bream iridovirus in transgenic rice callus. the rmcp in lyophilized rice callus powder was added to feed to induce intestinal mucosal immunity for protection against and/or to red ... | 2013 | 24021312 |
| codon-optimized expression of fish iridovirus capsid protein in yeast and its application as an oral vaccine candidate. | fish iridovirus causes systemic disease with high morbidity and mortality in various species of wild and farm-raised fish, resulting in severe economic losses. recently, frequent outbreaks of iridovirus infection have occurred among cultured fish in many asian countries, emphasizing the need for a protective vaccine programme or the development of a suitable therapy. in this study, we expressed a recombinant major capsid protein (rmcp) of rock bream iridovirus (rbiv) from yeast using codon optim ... | 2013 | 23488597 |
| construction and analysis of experimental dna vaccines against megalocytivirus. | iridoviruses are large double-stranded dna viruses with icosahedral capsid. the iridoviridae family contains five genera, one of which is megalocytivirus. megalocytivirus has emerged in recent years as an important pathogen to a wide range of marine and freshwater fish. in this study, we aimed at developing effective genetic vaccines against megalocytivirus affecting farmed fish in china. for this purpose, we constructed seven dna vaccines based on seven genes of rock bream iridovirus isolate 1 ... | 2012 | 22986024 |
| molecular insights of two stat1 variants from rock bream (oplegnathus fasciatus) and their transcriptional regulation in response to pathogenic stress, interleukin-10, and tissue injury. | signal transducers and activators of transcription 1 (stat1) is critically involved in mediating cytokine-driven signaling, and triggers the transcription of target genes to activate cellular functions. although the structural and functional aspects of stat members have been well described in mammals, only limited information is available for the stat genes in teleost fishes. in the present study, two variants of stat1 genes (rbstat1 and rbstat1l) were identified from rock bream and characterize ... | 2017 | 28818616 |
| cpg odn 1668 induce innate and adaptive immune responses in rock bream (oplegnathus fasciatus) against rock bream iridovirus (rbiv) infection. | rock bream iridovirus (rbiv) causes severe mass mortalities in rock bream in korea. cpg odn 1668 showed promise as immunoprotective agents against rbiv infection in rock bream. in this study, we assessed innate/adaptive-related gene expression patterns in rbiv-infected rock bream with and without cpg odn 1668 administration to determine important immune defence related factors that may affect fish survival. in the cpg odn 1668+virus-injected group, virus copies were more than 7.4- to 790591-fold ... | 2017 | 28860075 |
| protective immunity against rock bream iridovirus (rbiv) infection and tlr3-mediated type i interferon signaling pathway in rock bream (oplegnathus fasciatus) following poly (i:c) administration. | in this study, we evaluated the potential of poly (i:c) to induce antiviral status for protecting rock bream from rbiv infection. rock bream injected with poly (i:c) at 2 days before infection (1.1 × 10(4)) at 20 °c had significantly higher protection with rps 13.4% and 33.4% at 100 and 200 μg/fish, respectively, through 100 days post infection (dpi). the addition of boost immunization with poly (i:c) at before/post infection at 20 °c clearly enhanced the level of protection showing 33.4% and 60 ... | 2017 | 28602740 |
| protective immunity against megalocytivirus infection in rock bream (oplegnathus fasciatus) following cpg odn administration. | rock bream iridovirus (rbiv) disease in rock bream (oplegnathus fasciatus) remains an unsolved problem in korea aquaculture farms. cpg odns are known as immunostimulant, can improve the innate immune system of fish providing resistance to diseases. in this study, we evaluated the potential of cpg odns to induce anti-viral status protecting rock bream from different rbiv infection conditions. we found that, when administered into rock bream, cpg odn 1668 induces better antiviral immune responses ... | 2017 | 28579234 |
| expression analysis of interferon-stimulated gene 15 in the rock bream oplegnathus fasciatus against rock bream iridovirus (rsiv) challenge. | interferon-stimulated gene 15 (isg15) is known to interfere with viral replication and infection by limiting the viral infection of cells. interferon-stimulated gene 15 (isg15) interferes with viral replication and infectivity by limiting viral infection in cells. it also plays an important role in the immune response. in this study, tissue-specific expression of isg15 in healthy rock bream samples and spatial and temporal expression analysis of rock bream isg15 (rbisg15) were performed followin ... | 2017 | 29354783 |
| dna vaccine encoding myristoylated membrane protein (mmp) of rock bream iridovirus (rbiv) induces protective immunity in rock bream (oplegnathus fasciatus). | rock bream iridovirus (rbiv) causes severe mass mortalities in rock bream (oplegnathus fasciatus) in korea. in this study, we investigated the potential of viral membrane protein to induce antiviral status protecting rock bream against rbiv infection. we found that fish administered with orf008l (myristoylated membrane protein, mmp) vaccine exhibited significantly higher levels of survival compared to orf007l (major capsid protein, mcp). moreover, orf008l-based dna vaccinated fish showed signifi ... | 2018 | 29325821 |
| innate immune responses against rock bream iridovirus (rbiv) infection in rock bream (oplegnathus fasciatus) following poly (i:c) administration. | poly (i:c) showed promise as an immunoprotective agents in rock bream against rock bream iridovirus (rbiv) infection. in this study, we evaluated the time-dependent virus replication pattern and antiviral immune responses in rbiv-infected rock bream with and without poly (i:c) administration. in the poly (i:c)+virus-injected group, virus copy numbers were more than 18.9-, 24.0- and 479.2-fold lower than in the virus only injected group at 4 (4.73 × 104 and 8.95 × 105/μl, respectively), 7 (3.67 × ... | 2017 | 28986216 |
| rock bream iridovirus (rbiv) replication in rock bream (oplegnathus fasciatus) exposed for different time periods to susceptible water temperatures. | rock bream iridovirus (rbiv) is a member of the megalocytivirus genus that causes severe mortality to rock bream. water temperature is known to affect the immune system and susceptibility of fish to rbiv infection. in this study, we evaluated the time dependent virus replication pattern and time required to completely eliminate virus from the rock bream body against rbiv infection at different water temperature conditions. the rock bream was exposed to the virus and held at 7 (group a1), 4 (grou ... | 2017 | 28919266 |
| gene expression regulation of the tlr9 and myd88-dependent pathways in rock bream against rock bream iridovirus (rbiv) infection. | rock bream iridovirus (rbiv), which is a member of the megalocytivirus genus, causes severe mass mortalities in rock bream in korea. to date, the innate immune defense mechanisms of rock bream against rbiv is unclear. in this study, we assessed the expression levels of genes related to tlr9 and myd88-dependent pathways in rbiv-infected rock bream in high, low or no mortality conditions. in the high mortality group (100% mortality at 15 days post infection (dpi)), high levels of tlr9 and myd88 ex ... | 2017 | 28917488 |
| identification and molecular profiling of a novel homolog of cystatin c from rock bream (oplegnathus fasciatus) evidencing its transcriptional sensitivity to pathogen infections. | cystatins are reversible inhibitors of cysteine proteases which show an omnipresent distribution in the life on earth. although, cystatins with mammalian origin were well characterized and their roles in physiology were reported in details, those from teleostean origin are still underrepresented in literature. however, role of cystatins in fish physiology and immune defense is highlighted in few recent reports. in this study, a cystatin c holmologue from rock bream (oplegnathus fasciatus); terme ... | 2021 | 34041676 |