[lutzomyia peruensis shannon, 1929, possible natural vector of uta (cutaneous leishmaniasis)]. 19827156701
a comparative field study of the relative importance of lutzomyia peruensis and lutzomyia verrucarum as vectors of cutaneous leishmaniasis in the peruvian andes.a two-year field study of andean cutaneous leishmaniasis (uta) in the valley of purisima, ancash department, peru has provided quantitative epidemiologic and entomologic evidence for the predominant role of lutzomyia peruensis in the transmission of leishmania peruviana in this endemic area. the monthly incidence in the valley was greatest in the wet season (from december to may), when lu. peruensis was particularly endophilic. a significant correlation was detected between intradomiciliary (but ...19938357089
culture-independent species typing of neotropical leishmania for clinical validation of a pcr-based assay targeting heat shock protein 70 genes.pcr-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical leishmania species, as well as trypanosoma cruzi. the assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.200415131217
population genetic analysis of bartonella bacilliformis isolates from areas of peru where carrion's disease is endemic and epidemic.carrion's disease is caused by infection with the alpha-proteobacterium bartonella bacilliformis. distribution of the disease is considered coincident with the distribution of its known vector, the sand fly lutzomyia verrucarum. recent epidemics of b. bacilliformis infections associated with atypical symptomatology in nonendemic regions have raised questions regarding the historic and present distribution of this bacterium and the scope of disease that infection causes. phylogenetic relationship ...200415297516
adaptation and evaluation of the bottle assay for monitoring insecticide resistance in disease vector mosquitoes in the peruvian amazon.the purpose of this study was to establish whether the "bottle assay", a tool for monitoring insecticide resistance in mosquitoes, can complement and augment the capabilities of the established who assay, particularly in resource-poor, logistically challenging environments.200919728871
carrion's disease: more than a sand fly-vectored illness. 201627736978
colonization of lutzomyia verrucarum and lutzomyia longipalpis sand flies (diptera: psychodidae) by bartonella bacilliformis, the etiologic agent of carrión's disease.bartonella bacilliformis is a pathogenic bacterium transmitted to humans presumably by bites of phlebotomine sand flies, infection with which results in a bi-phasic syndrome termed carrión's disease. after constructing a low-passage gfp-labeled strain of b. bacilliformis, we artificially infected lutzomyia verrucarum and l. longipalpis populations, and subsequently monitored colonization of sand flies by fluorescence microscopy. initially, colonization of the two fly species was indistinguishabl ...201526436553
multiplex sybr® green-real time pcr (qpcr) assay for the detection and differentiation of bartonella henselae and bartonella clarridgeiae in cats.a novel sybr® green-real time polymerase chain reaction (qpcr) was developed to detect two bartonella species, b. henselae and b. clarridgeiae, directly from blood samples. the test was used in blood samples obtained from cats living in animal shelters in southern brazil. results were compared with those obtained by conventional pcr targeting bartonella spp. among the 47 samples analyzed, eight were positive using the conventional pcr and 12 were positive using qpcr. importantly, the new qpcr de ...024626408
shifts in the ecological niche of lutzomyia peruensis under climate change scenarios in peru.the peruvian andes presents a climate suitable for many species of sandfly that are known vectors of leishmaniasis or bartonellosis, including lutzomyia peruensis (diptera: psychodidae), among others. in the present study, occurrences data for lu. peruensis were compiled from several items in the scientific literature from peru published between 1927 and 2015. based on these data, ecological niche models were constructed to predict spatial distributions using three algorithms [support vector mac ...201728150865
genetic diversity of the mitochondrial cytochrome b gene in lutzomyia spp., with special reference to lutzomyia peruensis, a main vector of leishmania (viannia) peruviana in the peruvian andes.the genetic divergence caused by genetic drift and/or selection is suggested to affect the vectorial capacity and insecticide susceptibility of sand flies, as well as other arthropods. in the present study, cytochrome b (cyt b) gene sequences were determined in 13 species circulating in peru to establish a basis for analysis of the genetic structure, and the intraspecific genetic diversity was assessed in the lutzomyia (lu.) peruensis, a main vector species of leishmania (viannia) peruviana in p ...201323416127
natural infections of leishmania peruviana in animals in the peruvian andes.evidence that domestic dogs may act as reservoir hosts for cutaneous leishmaniasis in the peruvian andes is provided by the isolation, for the first time, from naturally infected dogs of parasites identified (by isoenzymes) as leishmania peruviana. leishmania parasites were isolated from nasal aspirates or biopsies from 5 (1.8%) of 279 asymptomatic dogs samples in endemic villages of the peruvian andes. in addition, leishmania (viannia) infections were identified in 15 (5.4%) of 276 nasal sample ...199910492779
cutaneous leishmaniasis in the peruvian andes: risk factors identified from a village cohort study.risk factors for cutaneous leishmaniasis were identified from a comparative study of transmission rates in 27 villages in the departments of lima, ancash, and piura in peru. to evaluate regression analysis as a tool for the incrimination of sand fly vectors in the absence of other biologic evidence, univariate and multivariate logistic regression analyses were used to identify which of 14 variables (the abundance of nine sand fly species, four social factors, and region) predicted transmission r ...19979063368
leishmania (viannia) peruviana isolated from the sandfly lutzomyia peruensis (diptera: psychodidae) and a sentinel hamster in the huayllacallán valley, ancash, peru. 19912068762
sugars in sandflies.the evidence for the acquisition of different types of sugars by wild caught sandflies is reviewed and the recent results summarised. studies on phlebotomus ariasi, p. perfiliewi and p. perniciosus in the mediterranean region has shown that these sandflies take honeydew derived sugars from aphids or coccids; in a similar study in the new world, lutzomyia peruensis has also been shown to have taken sugar from such sources. the use of high performance liquid chromatography (hplc) as a technique fo ...19911841240
description of lutzomyia (helcocyrtomyia) tolimensis, a new species of phlebotomine sandfly (diptera: psychodidae) from colombia.a description is presented of lutzomyia tolimensis sp. nov., a new species of the subgenus helcocyrtomyia, series sanguinaria. it was collected in dwellings, peridomestic environment and in nearby forest patches located in the foothills of the andean central cordillera, where in 2004-2006 occurred the largest epidemic ever recorded of leishmaniasis in colombia. the male of this species is differentiated from other members of the series sanguinaria based on the following combination of characters ...023295748
isolation and molecular identification of leishmania (viannia) peruviana from naturally infected lutzomyia peruensis (diptera: psychodidae) in the peruvian andes.leishmania (viannia) peruviana was isolated from 1/75 lutzomyia peruensis captured during may 2006 in an endemic cutaneous leishmaniasis region of the peruvian andes (chaute, huarochiri, lima, peru). sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. l. (viannia) sp. was determined by polymerase chain reaction (pcr), and leishmania species through molecular genotyping by pcr-restriction fragment length polymorphism analyses targeting the ge ...200717710315
transmission of leishmania metacyclic promastigotes by phlebotomine sand flies.a thorough understanding of the transmission mechanism of any infectious agent is crucial to implementing an effective intervention strategy. here, our current understanding of the mechanisms that leishmania parasites use to ensure their transmission from sand fly vectors by bite is reviewed. the most important mechanism is the creation of a "blocked fly" resulting from the secretion of promastigote secretory gel (psg) by the parasites in the anterior midgut. this forces the sand fly to regurgit ...200717517415
identification of bloodmeal sources of lutzomyia spp. in peru.bloodmeal sources of lutzomyia spp. were determined, using the bloodmeal analysis precipitin test, in chaute, lima, peru, an area endemic for cutaneous leishmaniasis (uta). from april 1990 to may 1991, sandflies were sampled in and around the village houses, with cdc light traps and a shannon trap with protected human bait, respectively. overall, 1424 sandflies were collected engorged. in indoor collections, human blood (n = 275) was the most frequent bloodmeal found, followed by cow (171) and c ...19947944678
[identification of leishmania species in patients and phlebotomines in transmission areas in a region of perú].to identify the species of leishmania present in the skin lesions of patients and lutzomyias living in endemic areas of la libertad, peru.201122086624
lutzomyia verrucarum can transmit leishmania peruviana, the aetiological agent of andean cutaneous much of the endemic area for cutaneous leishmaniasis (uta) in the peruvian andes, the only 2 anthropophilic sandfly species present are lutzomyia peruensis and lu. verrucarum. on the basis of a single confirmed isolation of leishmania peruviana (the aetiological agent of uta) from a wild lu. peruensis, and apparent associations between sandfly abundance and the incidence of uta, it is generally believed that lu. peruensis is the most important vector. in this paper, a potential role for lu. v ...19938266422
aphid derived sugars in the neotropical sandfly--lutzomyia is known that a sugar meal is a prerequisite for the transmission of leishmania by sandflies in the laboratory. lutzomyia peruensis the proven vector of leishmania peruviana, was caught by aspiration from crevices in rocks near chaute in the rimac valley, peru, cryopreserved and analysed for sugars using hplc. the major sugars present are glucose and fructose as well as smaller amounts of sucrose, maltose, melibiose, turanose and a trisaccharide, probably raffinose. the results indicate that ...19912052859
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