TitleAbstractYear(sorted ascending)
an olfactory receptor from apolygus lucorum (meyer-dur) mainly tuned to volatiles from flowering host plants.apolygus lucorum (meyer-dür) (hemiptera: miridae) is one of the most serious agricultural pests, feeding on a wide range of cultivated plants, including cotton, cereals and vegetables in the north of china. this insect can frequently switch between habitats and host plants over seasons and prefer plants in bloom. a. lucorum relies heavily on olfaction to locate its host plants finely discriminating different plant volatiles in the environment. despite its economical importance, research on the o ...026050917
flight potential of lygus lucorum (meyer-dür) (heteroptera: miridae).lygus lucorum (meyer-dür) is a key pest of bt cotton in china. this study reports on its flight potential examined by a flight-mill system. we found that 10-d-old mated females engaged in flight over the greatest distance (40.1 +/- 5.2 km) and duration (7.7 +/- 1.0 h) in 24-h flight assays in relation to age, sex, and mating status. optimum temperature for flight was 20 degrees c, and optimum relative humidity was 75% rh. flight potential of 10-d-old mated females under the optimum conditions (2 ...200718284721
lethal and sublethal effects of endosulfan on apolygus lucorum (hemiptera: miridae).various pesticides directly impact insect pest populations through acute toxicity (lethal) effects, but they also cause a range of indirect (sublethal) effects on pest physiology or behavior. in this study, we describe both lethal and sublethal effects of endosulfan on the survival, development, and fecundity of apolygus lucorum meyer-dür (hemiptera: miridae), an important pest of bacillus thuringiensis (bt) cotton, gossypium hirsutum l., in china. we quantify pesticide effects on adult mirids a ...200819133460
early season host plants of apolygus lucorum (heteroptera: miridae) in northern china.apolygus lucorum (meyer-dür) (heteroptera: miridae) has become a severe pest of cotton and many other crops in northern china as a result of the widespread adoption of bacillus thuringiensis (berliner) cotton, with a corresponding reduction of broad-spectrum insecticide application in cotton fields. from the middle of april to middle june, a. lucorum feeds and develops on other host plants before dispersing to cotton fields. effective suppression of a. lucorum populations before they enter cotto ...201223156156
establishment of an artificial diet for successive rearing of apolygus lucorum (hemiptera: miridae).the plant bug apolygus lucorum (meyer-dür) is a major pest on bt cotton and multiple crops in northern china. a modified ni artificial diet called t7 was developed to rear this pest for 10 successive generations both individually and in groups. this t7 diet was similar in components and preparation process to the ni diet used for lygus hesperus in north america, but was comprised of more whole chicken eggs, soybean lecithin, and vanderzant vitamin mixture, and less brewer's yeast, wheat germ, li ...201223356055
diapause induction in apolygus lucorum and adelphocoris suturalis (hemiptera: miridae) in northern china.with widespread planting of bt cotton and an associated reduction in the use of broad-spectrum insecticides, the mirid bugs apolygus lucorum (meyer-dür) and adelphocoris suturalis jakovlev (hemiptera: miridae) become major pests of cotton in northern china in recent years. both species overwinter as diapausing eggs that may enhance ability to survive the cold winter. the effects of photoperiod and temperature on diapause induction in a. lucorum and a. suturalis were investigated under laboratory ...201223321109
identification and binding characterization of three odorant binding proteins and one chemosensory protein from apolygus lucorum (meyer-dur).for the sucking insect, apolygus lucorum, taste is essential for finding host plants and oviposition sites. in a. lucorum, taste relies largely on the sensory system located within its proboscis. in this study, we constructed a cdna library from a. lucorum proboscises and conducted preliminary analysis of 1554 ests. from this collection, we identified three putative odorant-binding proteins (alucobp3, alucobp4, alucobp6) and one chemosensory protein (aluccsp1). quantitative real-time polymerase ...201223053914
assessment of physiological sublethal effects of imidacloprid on the mirid bug apolygus lucorum (meyer-dür).apolygus lucorum (meyer-dür) (hemiptera: miridae) is currently one of major mirid pests in the yangtze river and the yellow river regions in china. imidacloprid (neonicotinoid) is widely used against pierce-sucking pest insects, including against a. lucorum. in addition to its direct lethal effect, multiple negative sublethal effects may also occur in exposed insects. we assessed potential sublethal effects of imidacloprid on some biological characteristics of a. lucorum with the aim of increasi ...201222740097
functional characterizations of one odorant binding protein and three chemosensory proteins from apolygus lucorum (meyer-dur) (hemiptera: miridae) legs.chemoreception plays an important role in insects for sensing information when searching for host and oviposition sites. an understanding of the chemosensory mechanism could aid in the development of new methods to effectively prevent damage from insects in agriculture. we have constructed a legs cdna library for apolygus lucorum and sequenced 1584 ests, from which we identified 669 unigenes. from this collection we identified one putative odorant binding protein (alucobp5) and three chemosensor ...201323665333
preference of a polyphagous mirid bug, apolygus lucorum (meyer-dür) for flowering host plants.apolygus lucorum (meyer-dür) (hemiptera: miridae) is one of the most important herbivores in a broad range of cultivated plants, including cotton, cereals, vegetables, and fruit crops in china. in this manuscript, we report on a 6-year long study in which (adult) a. lucorum abundance was recorded on 174 plant species from 39 families from early july to mid-september. through the study period per year, the proportion of flowering plants exploited by adult a. lucorum was significantly greater than ...201323874835
identification and characterization of (e)-β-caryophyllene synthase and α/β-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton.cotton (gossypium hirsutum l.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. to understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, ghtps1 and ghtps2, were heterologously expressed and characterized. recombinant ghtps1 accepted farnesyl pyrophosphate as substrate and prod ...201324184450
combination of plant and insect eggs as food sources facilitates ovarian development in an omnivorous bug apolygus lucorum (hemiptera: miridae).diet nutrient is considered as an important regulatory factor for reproduction of insects. to understand the effect of different food sources on the reproductive physiology of apolygus lucorum (meyer-dür), the ovarian development in adult females was investigated when they were fed on green beans (gb), combination of green beans phaseolus vulgaris l and helicoverpa armigera eggs (gbhe), or h. armigera eggs (he). a female of a. lucorum has two ovaries, and each ovary contained seven yellowish ova ...201323865184
characterizing heat shock protein 90 gene of apolygus lucorum (meyer-dür) and its expression in response to different temperature and pesticide this study, we cloned a full-length cdna of heat shock protein (hsp) gene of apolygus lucorum (meyer-dür) [alhsp90, kc109781] and investigated its expression in response to temperature and pesticide stresses. the open reading frame (orf) of alhsp90 is 2,169 bp in length, encoding a 722 amino acid polypeptide with a predicted molecular weight of 82.99 kda. transcriptional and translational expression profiles of alhsp90 under extreme temperature or pesticide stresses were examined by fluoresce ...201424623316
seasonal migration of apolygus lucorum (hemiptera: miridae) over the bohai sea in northern china.during the past decade, apolygus lucorum (meyer-dür) has become a key pest on cotton in northern china. whether or not this species is a migrant, and if so, what pattern of seasonal migration this species exhibits remains unknown. the combination of searchlight trapping and radar observation on an isolated island in the center of bohai gulf during the past 11 yr provided direct evidence that both male and female a. lucorum adults migrate at least 40-60 km (and probably much greater distances) ac ...201425195428
plant volatile analogues strengthen attractiveness to leaf bug apolygus lucorum (meyer-dür) is one of the major pests in agriculture. management of a. lucorum was largely achieved by using pesticides. however, the increasing population of a. lucorum since growing bt cotton widely and the increased awareness of ecoenvironment and agricultural product safety makes their population-control very challenging. therefore this study was conducted to explore a novel ecological approach, synthetic plant volatile analogues, to manage the pest. here, pla ...201424911460
sublethal effects of the chitin synthesis inhibitor, hexaflumuron, in the cotton mirid bug, apolygus lucorum (meyer-dür).hexaflumuron is a type of benzoylphenylurea insecticide which is highly toxic for many insects. sublethal doses of hexaflumuron have been shown to significantly affect insect growth and development. however, the action mechanism of hexaflumuron is not well understood. in the present study, first instar apolygus lucorum nymphs were exposed to sublethal doses of hexaflumuron based on the estimated 120h acute lc50 valve of 20.53mg/ml. we found that exposure to sublethal hexaflumuron doses resulted ...201424861933
molecular characterization of soluble and membrane-bound trehalases in the cotton mirid bug, apolygus lucorum.trehalose, a major hemolymph sugar in insects, is hydrolyzed by trehalase. we identified a soluble and a membrane-bound form of trehalase and isolated the corresponding mrna, altre-1, and altre-2 in the cotton mirid bug, apolygus lucorum. the deduced amino acid sequences of altre-1 and altre-2 revealed mature proteins with 643 and 617 amino acids, respectively. altre-1 and altre-2 contained trehalase signature motifs, and altre-2 contained a putative transmembrane domain near the c-terminus, sug ...201424740925
identification of the key weather factors affecting overwintering success of apolygus lucorum eggs in dead host tree branches.understanding the effects of weather on insect population dynamics is crucial to simulate and forecast pest outbreaks, which is becoming increasingly important with the effects of climate change. the mirid bug apolygus lucorum is an important pest on cotton, fruit trees and other crops in china, and primarily lays its eggs on dead parts of tree branches in the fall for subsequent overwintering. as such, the eggs that hatch the following spring are most strongly affected by ambient weather factor ...201424705353
silencing in apolygus lucorum of the olfactory coreceptor orco gene by rna interference induces eag response declining to two putative semiochemicals.apolygus lucorum (meyer-dür) (hemiptera: miridae) is an agronomically important pest that causes severe economic damage to the cotton, fruit, and vegetable industries. similar to other insects, a. lucorum can perceive and discriminate olfactory cues. a highly conserved and broadly expressed olfactory coreceptor (orco) is crucial for insect olfaction, and orco orthologs have been identified in several insect species. in this study, a homology-based polymerase chain reaction (pcr) method was utili ...201424216470
the mitochondrial genome of the plant bug apolygus lucorum (hemiptera: miridae): presently known as the smallest in heteroptera.the complete mitochondrial (mt) genome of the plant bug, apolygus lucorum, an important cotton pest, has been sequenced and annotated in this study. the entire circular genome is 14 768 bp in size and represents the smallest in presently known heteropteran mt genomes. the mt genome is encoding for two ribosomal rna (rrna) genes, 22 transfer rna (trna) genes, 13 protein coding genes and a control region, and the order, content, codon usage and base organization show similarity to a great extent t ...201423956187
laboratory evaluation of acute toxicity of the essential oil of allium tuberosum leaves and its selected major constituents against apolygus lucorum (hemiptera: miridae).the aim of this research was to evaluate acute toxicity of the essential oil of leaves of chinese chives, allium tuberosum rottler ex spreng (asparagales: alliaceae) and its major constituents against apolygus lucorum meyer-dür (hemiptera: miridae). the essential oil of a. tuberosum leaves was obtained by hydrodistillation and analyzed by gas chromatography and gas chromatography-mass spectrometry. the major constituents of the oil were sulfur-containing compounds, including allyl methyl trisulf ...201526254289
molecular characterization and expression profiling of odorant-binding proteins in apolygus lucorum.apolygus lucorum (meyer-dür) (hemiptera: miridae) is one of the most important agricultural pests, with broad host range and cryptic feeding habits in china. chemosensory behavior plays an important role in many crucial stages in the life of a. lucorum, such as the detection of sex pheromone cues during mate pursuit and fragrant odorants during flowering host plant localization. odorant-binding proteins (obps) are involved in the initial biochemical recognition steps in semiochemical perception. ...201526466366
volatile fragrances associated with flowers mediate host plant alternation of a polyphagous mirid bug.apolygus lucorum (hemiptera: miridae) is an important insect pest of cotton and fruit trees in china. the adults prefer host plants at the flowering stage, and their populations track flowering plants both spatially and temporally. in this study, we examine whether flower preference of its adults is mediated by plant volatiles, and which volatile compositions play an important role in attracting them. in olfactometer tests with 18 key host species, the adults preferred flowering plants over non- ...201526423224
a point mutation in the acetylcholinesterase-1 gene is associated with chlorpyrifos resistance in the plant bug apolygus lucorum.control of chinese apolygus lucorum relies heavily on organophosphate insecticides. here we describe resistance to the organophosphate chlorpyrifos in an a. lucorum strain, bz-r, which was developed from a field-collected strain (bz) by selection with chlorpyrifos in the laboratory. bz-r showed 21-58 fold resistance to chlorpyrifos compared with the laboratory reference strain lsf and another susceptible strain, bz-s, derived from bz. bz-r also showed several fold resistance to two other organop ...201526363297
ecdysone receptor isoform-b mediates soluble trehalase expression to regulate growth and development in the mirid bug, apolygus lucorum (meyer-dür).ecdysone receptor (ecr) is the hormonal receptor of ecdysteroids and strictly regulates growth and development in insects. however, the action mechanism of ecr is not very clear. in this study, the cdna of ecr isoform-b was cloned from apolygus lucorum (alecr-b) and its expression profile was investigated. we reduced alecr-b mrna expression using systemic rna interference in vivo, and obtained knockdown specimens. examination of these specimens indicated that alecr-b is required for nymphal surv ...201526335337
molecular and functional characterization of the ecdysone receptor isoform-a from the cotton mirid bug, apolygus lucorum (meyer-dür).we cloned the cdna of the ecdysone receptor (ecr) isoform-a from the mirid bug, apolygus lucorum (alecr-a). the alecr-a cdna has an open reading frame of 1410 bp with a conserved sequence of approximately 20 amino acids at the carboxyl-end of its a/b-specific domain. phylogenetic analysis showed that alecr-a is very similar to the ecr-a genes of other hemiptera species. alecr-a mrna was detected at all developmental stages of a. lucorum with peaks correlating to ecdysteroid pulses. alecr-a was a ...201526238700
sex pheromones and reproductive isolation in five mirid species.mate location in many mirid bugs (heteroptera: miridae) is mediated by female-released sex pheromones. to elucidate the potential role of the pheromones in prezygotic reproductive isolation between sympatric species, we investigated differences in the pheromone systems of five mirid species, apolygus lucorum, apolygus spinolae, orthops campestris, stenotus rubrovittatus and taylorilygus apicalis. gc/ms analyses of metathoracic scent gland extracts of virgin females showed that all five species p ...201525973902
molecular characterization and expression profiles of polygalacturonase genes in apolygus lucorum (hemiptera: miridae).polygalacturonase (pg) is an enzyme in the salivary glands of piercing-sucking mirid bugs (hemiptera: miridae) that plays a key role in plant feeding and injury. by constructing a full-length cdna library, we cloned and characterized 14 pg genes from the salivary glands of apolygus lucorum, a pestiferous mirid bug in cotton, fruit trees and other crops in china. blast search analysis showed that the amino acid sequences deduced from transcripts of the pg genes were closely related to pgs from ot ...201525955307
seasonal alterations in host range and fidelity in the polyphagous mirid bug, apolygus lucorum (heteroptera: miridae).in herbivorous insects, host plant switching is commonly observed and plays an important role in their annual life cycle. however, much remains to be learned about seasonal host switching of various pestiferous arthropods under natural conditions. from 2006 until 2012, we assessed apolygus lucorum (meyer-dür) host plant use in successive spring, summer and winter seasons at one single location (langfang, china). data were used to quantify changes in host plant breadth and host fidelity between s ...201525692969
identification of heat shock cognate protein 70 gene (alhsc70) of apolygus lucorum and its expression in response to different temperature and pesticide stresses.heat shock cognate protein 70 (hsc70) is a very important stress-resistance protein of insects against environmental stresses. we employed fluorescent real-time quantitative polymerase chain reaction and western-blot techniques to analyze the transcriptional and translational expression profiles of alhsc70 under extreme temperature (4°c and 40°c) or 4 pesticide stresses in apolygus lucorum. the results showed that the expression of alhsc70 were significantly induced by cyhalothrin or extremely h ...201625448821
a de novo transcriptomic analysis to reveal functional genes in apolygus lucorum.the widespread planting of genetically engineered cotton producing the cry1ac toxin has led to significantly reduced pesticide applications since 1997. however, consequently, the number of green mirid bugs (gmb), apolygus lucorum (meyer-dür) has increased. so far the gmb, instead of the cotton bollworm helicoverpa armigera (hübner), has become the major pest in the transgenic bt cotton field and has influenced cotton yield. disproportionately, only a few studies on gmb at molecular level have be ...201625408247
the joint toxicity of different temperature coefficient insecticides on apolygus lucorum (hemiptera: miridae).the effect of temperature on the cotoxicity coefficient (ctc) value was used to evaluate mixture efficacy of different temperature coefficient chemicals from 15 to 35°c by exposing third-instar apolygus lucorum (meyer-dür) to dip-treated asparagus bean pods. the results indicated the joint toxicity of same temperature coefficient insecticide (tci) types were unaffected by temperature. this means that even when temperatures change, the mixture ratios of the highest ctc values remained the same, a ...201627190041
targeting insect mitochondrial complex i for plant protection.plant engineered to express double-stranded rna (dsrna) can target the herbivorous insect gene for silencing. although mounting evidence has emerged to support feasibility of this new pest control technology, field application is slow largely due to lack of potent targets. here, we show that suppression of the gene encoding ndufv2, a subunit of mitochondrial complex i that catalyses nadh dehydrogenation in respiratory chain, was highly lethal to insects. feeding cotton bollworm (helicoverpa armi ...201626914579
a point mutation (l1015f) of the voltage-sensitive sodium channel gene associated with lambda-cyhalothrin resistance in apolygus lucorum (meyer-dür) population from the transgenic bt cotton field of china, the green mirid bug, apolygus lucorum (meyer-dür), has caused severe economic damage to many kinds of crops, especially the cotton and jujubes. pyrethroid insecticides have been widely used for controlling this pest in the transgenic bt cotton field. five populations of a. lucorum collected from cotton crops at different locations in china were evaluated for lambda-cyhalothrin resistance. the results showed that only the population collected from shandong province exhibited 30-fold of ...201626821662
levels of salivary enzymes of apolygus lucorum (hemiptera: miridae), from 1st instar nymph to adult, and their potential relation to bug recent years, apolygus lucorum has caused increasing damage to cotton and fruit trees in china. the salivary enzymes secreted by a. lucorum when sucking on host plants induce a series of biochemical reactions in plants, and the pre-oral digestion benefits the bug feeding. in this study, the food intake of a. lucorum from 1st instar nymphs to adults was measured, and the corresponding salivary activity of pectinase, amylase, cellulase, protease, polyphenol oxidase and peroxidase was determined ...201628002486
effect of water on survival and development of diapausing eggs of apolygus lucorum (hemiptera: miridae).the green mirid bug apolygus lucorum is a regional pest of multiple crops in northern china, and the survival and development of diapausing eggs during winter plays an important role in the population dynamics of this species. the effect of water on the survival and development of a. lucorum eggs was investigated using laboratory-induced diapause. diapausing eggs were exposed to various humidity regimes under three conditions: (1) termination of diapause with exposure to warm long-day (wld) cond ...201627603210
survey of organophosphate resistance and an ala216ser substitution of acetylcholinesterase-1 gene associated with chlorpyrifos resistance in apolygus lucorum (meyer-dür) collected from the transgenic bt cotton fields in china.the mirid bug is frequently controlled by the application of organophosphorus insecticides in the transgenic bt cotton field of china. a topical bioassay method was performed to evaluate the toxicities of chlorpyrifos and malathion towards field-collected chinese populations of apolygus lucorum from transgenic bt cotton fields. for chlorpyrifos, the resistance ratios ranged from 0.8 to 9.4-fold compared to a susceptible strain. for malathion, the resistance levels relative to the susceptible str ...201627521910
a female-biased odorant receptor from apolygus lucorum (meyer-dür) tuned to some plant odors.apolygus lucorum (meyer-dür) (hemiptera: miridae) is a serious pest of cotton, jujube, grape and many other crops around the world. understanding how olfactory information directs this insect to its host plants may provide environment-friendly approaches to the control of its population in agriculture. in our study, we cloned an odorant receptor gene, alucor46, that was specifically expressed in antennae and female-biased. functional expression of alucor46 in xenopus oocytes showed that it is tu ...201627483241
central projection of antennal sensory neurons in the central nervous system of the mirid bug apolygus lucorum (meyer-dür).the mirid bug apolygus lucorum (meyer-dür), a polyphagous pest, is dependent on olfactory cues to locate various host plant species and mates. in this study, we traced the projection pathway of the antennal sensory neurons and visualized their projection patterns in the central nervous system of a. lucorum through confocal microscopy and digital reconstructions. we also examined the glomerular organization of the primary olfactory center of the brain, the antennal lobe, and created a three-dimen ...201627478892
identification and expression analysis of an olfactory receptor gene family in green plant bug apolygus lucorum (meyer-dür).olfactory receptors are believed to play a central role in insects host-seeking, mating, and ovipositing. on the basis of male and female antennal transcriptome of adult apolygus lucorum, a total of 110 candidate a. lucorum odorant receptors (alucor) were identified in this study including five previously annotated alucors. all the sequences were validated by cloning and sequencing. tissue expression profiles analysis by rt-pcr indicated most alucors were antennal highly expressed genes. the qpc ...201627892490
molecular characterisation of the vitellogenin gene (alvg) and its expression after apolygus lucorum had fed on different hosts.the polyphagous pest apolygus lucorum is now the dominant pest of bacillus thuringiensis (bt) cotton in china. in this study, the transcriptional and translational profiles of alvg influenced by different hosts were identified, and then the correlations between alvg gene or alvg protein expression and key population proliferation parameters of a. lucorum were further clarified.201626663893
identification and functional characterization of four transient receptor potential ankyrin 1 variants in apolygus lucorum (meyer-dür).as signal integrators that respond to various physical and chemical stimuli, transient receptor potential (trp) channels fulfil critical functional roles in the sensory systems of both vertebrate and invertebrate organisms. here, four variants of trp ankyrin 1 (trpa1) were identified and cloned from the green plant bug, apolygus lucorum. spatiotemporal expression profiling across development and in different adult tissues revealed that the highest relative-transcript levels occurred in first-ins ...201627038267
the specific host plant dna detection suggests a potential migration of apolygus lucorum from cotton to mungbean fields.the polyphagous mirid bug apolygus lucorum (heteroptera: miridae) has more than 200 species of host plants and is an insect pest of important agricultural crops, including cotton (gossypium hirsutum) and mungbean (vigna radiata). previous field trials have shown that a. lucorum adults prefer mungbean to cotton plants, indicating the considerable potential of mungbean as a trap crop in cotton fields. however, direct evidence supporting the migration of a. lucorum adults from cotton to mungbean is ...201728586352
activities of digestive enzymes in the omnivorous pest apolygus lucorum (hemiptera: miridae).the mirid bug apolygus lucorum meyer-dür, 1843, an omnivorous species that feeds on plants and animals, has become a major pest in china as production of bt-cotton has grown to such a large scale. its omnivory is likely to be critical for its success, but the digestive mechanism(s) underlying processing and adsorption of such diverse foods is relatively unknown. here, we examined the activities of digestive enzymes of a. lucorum in the salivary gland complex and midgut and the effect of sex, age ...201728039425
seed treatment combined with a spot application of clothianidin granules prolongs the efficacy of controlling piercing-sucking insect pests in cotton fields.seed treatments can directly protect cotton from early season piercing-sucking insect aphis gossypii glover but hardly provide long-term protection against apolygus lucorum (meyer-dür). therefore, the efficacy of clothianidin seed treatments combined with spot applications of clothianidin granules at the bud stage of cotton was evaluated to control piercing-sucking pests during the entire cotton growing season. clothianidin seed treatments (at the rate of 4 g ai/kg seed) combined with a clothian ...201728837321
transcriptome analysis of three cotton pests reveals features of gene expressions in the mesophyll feeder apolygus lucorum.the green mirid bug apolygus lucorum is an agricultural pest that is known to cause damage to more than 150 plant species. here, we report the transcriptomes of a. lucorum at three different developmental stages (the second and fifth instar nymphs and adults). a total of 98,236 unigenes with an average length of 1,335 nt was obtained, of which 50,640 were annotated, including those encoding digestive enzymes and cytochrome p450s. comparisons with cotton bollworm and cotton aphid transcriptomes r ...201728730342
the nuclear hormone receptor e75a regulates vitellogenin gene (al-vg) expression in the mirid bug apolygus lucorum.apolygus lucorum is the predominant pest of bacillus thuringiensis (bt) cotton in china. 20-hydroxyecdysone (20e) plays a key role in the reproduction of this insect. to better understand the mechanism underlying 20e-regulated reproduction, the nuclear hormone receptor e75 isoform-a of ap. lucorum (al-e75a) was cloned and its expression analysed. a 2241-bp sequence of al-e75a cdna encoded an open reading frame of a polypeptide with a predicted molecular mass of 69.04 kda. al-e75a mrna was detect ...201729193387
nitenpyram seed treatment effectively controls against the mirid bug apolygus lucorum in cotton seedlings.the mirid bug apolygus lucorum (meyer-dür) has become a major pest in cotton fields and has led to significant yield losses due to the widespread use of transgenic bt cotton in china. eight neonicotinoid seed treatments were investigated to determine their effects on the management of a. lucorum in cotton fields. all neonicotinoid seed treatments reduced the cotton damage caused by a. lucorum, and nitenpyram at the rate of 4 g/kg seed showed the most favorable efficacy in suppressing a. lucorum ...201728819325
juvenile hormone epoxide hydrolase: a promising target for hemipteran pest management.juvenile hormone epoxide hydrolase (jheh) has attracted great interest because of its critical role in the regulation of juvenile hormone (jh) in insects. in this study, one jheh gene from apolygus lucorum (alucjheh) was characterized in terms of deduced amino acid sequence, phylogeny, homology modeling and docking simulation. the results reveals a conserved catalytic mechanism of alucjheh toward jh. our study also demonstrates that the mrna of alucjheh gene was detectable in head, thorax and ab ...201728400585
functional analysis of two polygalacturonase genes in apolygus lucorum associated with eliciting plant injury using rna interference.salivary enzymes of many piercing-sucking insects lead to host plant injury. the salivary enzymes, polygalacturonase (pgs), act in insect feeding. pg family genes have been cloned from the mirid bug apolygus lucorum, a pest of cotton and other host crops in china. we investigated the function of two pg genes that are highly expressed in a. lucorum nymphs (pg3-4) and adults (pg3-5), using sirna injection-based rna interference (rnai). accumulation of mrna encoding both genes and their cognate pro ...201728370316
predation by generalist arthropod predators on apolygus lucorum (hemiptera: miridae): molecular gut-content analysis and field-cage assessment.the mirid bug apolygus lucorum (meyer-dür) is a principal pest of cotton that also causes great damage to many other crops in china. a study was conducted to assess the mortality of a. lucorum from generalist arthropod predators using both molecular methods and a field-cage trial. the species-specific primer pair for the detection of a. lucorum tissues in predators was designed according to the sequences of the cytochrome oxidase subunit i (coi) gene.201727349598
comparative mitogenomic analysis of mirid bugs (hemiptera: miridae) and evaluation of potential dna barcoding markers.the family miridae is one of the most species-rich families of insects. to better understand the diversity and evolution of mirids, we determined the mitogenome of lygus pratenszs and re-sequenced the mitogenomes of four mirids (i.e., apolygus lucorum, adelphocoris suturalis, ade. fasciaticollis and ade. lineolatus). we performed a comparative analysis for 15 mitogenomic sequences representing 11 species of five genera within miridae and evaluated the potential of these mitochondrial genes as mo ...201728791201
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