loktanella tamlensis sp. nov., a novel member of the alphaproteobacteria, isolated from aerobic, gram-negative, chemoorganotrophic bacterium, designated strain ssw-35t, was isolated from seawater in jeju, republic of korea. cells were motile, short rods; colonies were circular, smooth, convex, translucent and light brown in colour. no diffusible pigment is formed on any of tested media. the temperature and ph ranges for growth were 4-30°c and ph 7.1-10.1. 16s rrna gene sequence analyses showed that the organism belonged to the genus loktanella, with the phylogenetically close re ...201121515703
the structural diversity of carbohydrate antigens of selected gram-negative marine bacteria.marine microorganisms have evolved for millions of years to survive in the environments characterized by one or more extreme physical or chemical parameters, e.g., high pressure, low temperature or high salinity. marine bacteria have the ability to produce a range of biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents, and as a result, they have been a topic of research interest for many years. among these biologically active molecules, t ...201122073003
against the rules: a marine bacterium, loktanella rosea, possesses a unique lipopolysaccharide.bacteria are an inimitable source of new glyco-structures potentially useful in medicinal and environmental chemistry. lipopolysaccharides (lps; endotoxins) are the major components of the outer membrane of gram-negative bacteria; being exposed toward the external environment they can undergo structural changes and thus, they often possess peculiar chemical features that allow them to thrive in harsh chemical and physical environments. marine bacteria have evolved and adapted over millions of ye ...201020093711
mameliella alba gen. nov., sp. nov., a marine bacterium of the roseobacter clade in the order rhodobacterales.a gram-negative, non-motile, rod-shaped bacterial strain, jlt354-w(t), that accumulates poly-beta-hydroxybutyrate granules was isolated from the south china sea. phylogenetic analysis based on 16s rrna gene sequences showed that the strain was related to members of the genera antarctobacter, sagittula, oceanicola and loktanella; levels of similarity between strain jlt354-w(t) and members of the above genera were less than 92.0 %. the predominant fatty acid of strain jlt354-w(t) was c(18 : 1)omeg ...201019661508
low temperature s(0) biomineralization at a supraglacial spring system in the canadian high arctic.elemental sulfur (s(0) ) is deposited each summer onto surface ice at borup fiord pass on ellesmere island, canada, when high concentrations of aqueous h(2) s are discharged from a supraglacial spring system. 16s rrna gene clone libraries generated from sulfur deposits were dominated by β-proteobacteria, particularly ralstonia sp. sulfur-cycling micro-organisms such as thiomicrospira sp., and ε-proteobacteria such as sulfuricurvales and sulfurovumales spp. were also abundant. concurrent cultivat ...201121592302
mesaconyl-coenzyme a hydratase, a new enzyme of two central carbon metabolic pathways in bacteria.the coenzyme a (coa)-activated c5-dicarboxylic acids mesaconyl-coa and beta-methylmalyl-coa play roles in two as yet not completely resolved central carbon metabolic pathways in bacteria. first, these compounds are intermediates in the 3-hydroxypropionate cycle for autotrophic co2 fixation in chloroflexus aurantiacus, a phototrophic green nonsulfur bacterium. second, mesaconyl-coa and beta-methylmalyl-coa are intermediates in the ethylmalonyl-coa pathway for acetate assimilation in various bacte ...200818065535
microbial characterization of a subzero, hypersaline methane seep in the canadian high arctic.we report the first microbiological characterization of a terrestrial methane seep in a cryo-environment in the form of an arctic hypersaline (∼24% salinity), subzero (-5 °c), perennial spring, arising through thick permafrost in an area with an average annual air temperature of -15 °c. bacterial and archaeal 16s rrna gene clone libraries indicated a relatively low diversity of phylotypes within the spring sediment (shannon index values of 1.65 and 1.39, respectively). bacterial phylotypes were ...201020445635
bacterial diversity in the oxygen minimum zone of the eastern tropical south pacific.the structure and diversity of bacterial communities associated with the oxygen minimum zone (omz) of the eastern tropical south pacific was studied through phylogenetic analysis. clone libraries of 16s rrna gene fragments were constructed using environmental dna collected from the omz (60 m and 200 m), the sea surface (10 m), and the deep oxycline (450 m). at the class level, the majority of sequences affiliated to the gamma- (53.7%) and alpha-proteobacteria (19.7%), and to the bacteroidetes (1 ...200818294206
molecular genetic analysis of a dimethylsulfoniopropionate lyase that liberates the climate-changing gas dimethylsulfide in several marine alpha-proteobacteria and rhodobacter sphaeroides.the alpha-proteobacterium sulfitobacter ee-36 makes the gas dimethylsulfide (dms) from dimethylsulfoniopropionate (dmsp), an abundant antistress molecule made by many marine phytoplankton. we screened a cosmid library of sulfitobacter for clones that conferred to other bacteria the ability to make dms. one gene, termed dddl, was sufficient for this phenotype when cloned in pet21a and introduced into escherichia coli. close dddl homologues exist in the marine alpha-proteobacteria fulvimarina, lok ...200818237308
abundance, depth distribution, and composition of aerobic bacteriochlorophyll a-producing bacteria in four basins of the central baltic sea.the abundance, vertical distribution, and diversity of aerobic anoxygenic phototrophic bacteria (aap) were studied at four basins of the baltic sea. aap were enumerated by infrared epifluorescence microscopy, and their diversity was analyzed by using pufm gene clone libraries. in addition, numbers of cfu containing the pufm gene were determined, and representative strains were isolated. both approaches indicated that aap reached maximal abundance in the euphotic zone. maximal aap abundance was 2 ...200818502937
loktanella pyoseonensis sp. nov., isolated from beach sand, and emended description of the genus loktanella.a novel gram-stain-negative, aerobic, heterotrophic, obligately halophilic bacterium, designated strain jjm85(t), was isolated from beach sand in jeju, republic of korea. cells were rod-shaped and motile by means of flagella; colonies were pink, convex and smooth with an entire edge. the organism grew at ph 5.0-10.0 and 4-30 degrees c. phylogenetic analysis based on 16s rrna gene sequences showed that the organism belonged to the genus loktanella of the class alphaproteobacteria and formed a tig ...201019656928
antibacterial and antilarval-settlement potential and metabolite profiles of novel sponge-associated marine this study, we screened seven novel sponge-associated marine bacteria for their antibacterial and antilarval-settlement activity in order to find possible new sources of non-toxic or less toxic bioactive antifoulants. the anti-bacterial-growth activity of crude extracts of each bacterium was evaluated by the disk-diffusion assay. extracts of four potent bacteria with high and broad spectra of antibacterial activity were further separated with solvents of different polarities (hexane and ethyl ...200919471982
loktanella maricola sp. nov., isolated from seawater of the east sea in korea.a gram-negative, non-motile and rod-shaped loktanella-like bacterial strain, dsw-18(t), was isolated from seawater of the east sea, korea, and its exact taxonomic position was investigated by using a polyphasic approach. strain dsw-18(t) grew optimally at ph 7.0-8.0 and 25 degrees c in the presence of 2 % (w/v) nacl. it contained q-10 as the predominant ubiquinone and c(18 : 1) omega 7c as the major fatty acid. the major polar lipids were phosphatidylcholine, phosphatidylglycerol and diphosphati ...200717684260
loktanella hongkongensis sp. nov., a novel member of the alpha-proteobacteria originating from marine biofilms in hong kong waters.a gram-negative, non-motile, non-spore-forming, short rod-shaped bacterium (ust950701-009p(t)) was isolated from a marine biofilm in hong kong waters. colonies are pink in colour, convex with a smooth surface and entire edge. brown diffusible pigment is produced. whitish colonies, with otherwise identical morphology, emerge from every culture upon ageing. the white colonies can be maintained as separate cultures (ust950701-009w) without turning pink. ust950701-009p(t) and ust950701-009w have ide ...200415545471
loktanella koreensis sp. nov., isolated from sea sand in korea.a bacterial strain, ga2-m3(t), isolated from a sea-sand sample in korea, was subjected to polyphasic taxonomic characterization. cells of strain ga2-m3(t) were gram-negative, non-motile, non-spore-forming and short rod- to ovoid-shaped. comparative 16s rrna gene sequencing studies confirmed that the bacterium fell within the radiation of the genus loktanella. similarity levels between the 16s rrna gene sequence of strain ga2-m3(t) and those of type strains of loktanella species with validly publ ...200616957121
flow-cytometric cell sorting and subsequent molecular analyses for culture-independent identification of bacterioplankton involved in dimethylsulfoniopropionate transformations.marine bacterioplankton transform dimethylsulfoniopropionate (dmsp) into the biogeochemically important and climatically active gas dimethylsulfide. in order to identify specific bacterial taxa mediating dmsp processing in a natural marine ecosystem, we amended water samples from a southeastern u.s. salt marsh with 20 microm dmsp and tracked community shifts with flow cytometry (fcm) coupled to 16s rrna gene analyses. in two out of four seasons studied, dmsp amendments induced the formation of d ...200515746343
loktanella atrilutea sp. nov., isolated from seawater in japan.a gram-negative, rod-shaped bacterium, ig8(t), was isolated from seawater off the sanriku coast, japan. phylogenetic analysis based on 16s rrna gene sequences showed that strain ig8(t) represented a separate lineage within the genus loktanella; the highest 16s rrna gene sequence similarity values were found with the type strains of loktanella salsilacus (98.6 %) and loktanella fryxellensis (98.4 %). dna-dna hybridization values between strain ig8(t) and the type strains of l. salsilacus (27.9-36 ...200717766856
loktanella agnita sp. nov. and loktanella rosea sp. nov., from the north-west pacific whitish and four pinkish strains of gram-negative, non-motile, aerobic bacteria were isolated from sea-water and sediment samples collected in chazhma bay (sea of japan, pacific ocean). analysis of 16s rrna gene sequences revealed that these strains belonged to the 'alphaproteobacteria', having highest sequence similarity of about 94-97% with species of the genus loktanella. none of the strains degraded gelatin, casein, chitin, agar, dna or starch and they had limited ability to utilize carb ...200516166733
yangia pacifica gen. nov., sp. nov., a novel member of the roseobacter clade from coastal sediment of the east china aerobic, gram-negative bacterial isolate, strain dx5-10t, was isolated from coastal sediment of the east china sea. the taxonomy of strain dx5-10t was studied by phenotypic and phylogenetic methods. strain dx5-10t was motile, formed faint-yellowish colonies and was positive for catalase reaction and weakly positive for oxidase reaction. the nearly complete 16s rrna gene of strain dx5-10t was obtained and sequence analysis indicated that strain dx5-10t represented an independent lineage within ...200616514022
fine structural aspects of an embryo-adapted strain of eimeria tanella in the epithelial cells of the chorio-allantoic membrane.the fine structure and development of the endogenous stages of an embryo-adapted strain of eimeria tenella in chicken embryos is described. first-generation merozoites were formed by endodyogeny in uninucleate developmental stages as well as schizogony and the endoplasmic reticulum of merozoites of the second-generation schizonts appeared as a circular structure, often enclosing other organelles. in contrast to the parent strain, development of the second-generation schizonts was restricted to c ...1978652387
bacterial communities in the initial stage of marine biofilm formation on artificial surfaces.succession of bacterial communities during the first 36 h of biofilm formation in coastal water was investigated at 3 approximately 15 h intervals. three kinds of surfaces (i.e., acryl, glass, and steel substratum) were submerged in situ at sacheon harbor, korea. biofilms were harvested by scraping the surfaces, and the compositions of bacterial communities were analyzed by terminal restriction fragment length polymorphism (t-rflp), and cloning and sequencing of 16s rrna genes. while community s ...200818545967
loktanella salsilacus gen. nov., sp. nov., loktanella fryxellensis sp. nov. and loktanella vestfoldensis sp. nov., new members of the rhodobacter group, isolated from microbial mats in antarctic lakes.a taxonomic study was performed on 26 strains isolated from microbial mats in antarctic lakes of the vestfold hills and the mcmurdo dry valleys. phylogenetic analysis based on 16s rrna gene sequences placed these strains within the rhodobacter group of the alpha-subclass of the proteobacteria. sequence similarity values for the strains with their nearest phylogenetic neighbours (jannaschia, octadecabacter and ketogulonicigenium) ranged between 94.0 and 95.8%. dna-dna hybridizations and compariso ...200415280301
the chafer pheromone buibuilactone and ant pyrazines are also produced by marine bacteria.headspace extracts obtained from agar plate cultures of two marine bacteria from the north sea (germany), loktanella strain bio-204 and dinoroseobacter shibae strain dfl-27, were analyzed by gc-ms. several gamma-lactones and one delta-lactone were identified, besides pyrazines and some sulfur compounds. the absolute configuration of the major lactone (r,z)-dodec-5-en-4-olide, known as buibuilactone, a pheromone of several scarab beetles, was determined by a new catalytic enantioselective synthes ...200516124260
Displaying items 1 - 23 of 23