| unique genome replication mechanism of the archaeal virus afv1. | the exceptional genomic content and genome organization of the acidianus filamentous virus 1 (afv1) that infects the hyperthermophilic archaeon acidianus hospitalis suggest that this virus might exploit an unusual mechanism of genome replication. an analysis of replicative intermediates of the viral genome by two-dimensional (2d) agarose gel electrophoresis revealed that viral genome replication starts by the formation of a d-loop and proceeds via strand displacement replication. characterizatio ... | 2014 | 24779456 |
| orf157 from the archaeal virus acidianus filamentous virus 1 defines a new class of nuclease. | acidianus filamentous virus 1 (afv1) (lipothrixviridae) is an enveloped filamentous virus that was characterized from a crenarchaeal host. it infects acidianus species that thrive in the acidic hot springs (>85 degrees c and ph <3) of yellowstone national park, wy. the afv1 20.8-kb, linear, double-stranded dna genome encodes 40 putative open reading frames whose sequences generally show little similarity to other genes in the sequence databases. because three-dimensional structures are more cons ... | 2010 | 20200253 |
| acidianus filamentous virus 1 coat proteins display a helical fold spanning the filamentous archaeal viruses lineage. | acidianus filamentous virus 1 (afv1), a member of the lipothrixviridae family, infects the hyperthermophilic, acidophilic crenarchaeaon acidianus hospitalis. the virion, covered with a lipidic outer shell, is 9,100-a long and contains a 20.8-kb linear dsdna genome. we have identified the two major coat proteins of the virion (mcps; 132 and 140 amino acids). they bind dna and form filaments when incubated with linear dsdna. a c-terminal domain is identified in their crystal structure with a four- ... | 2009 | 19934032 |
| the thermo- and acido-stable orf-99 from the archaeal virus afv1. | acidianus filamentous virus 1 (afv1), isolated from acidic hot springs, is an enveloped lipid-containing archaeal filamentous virus with a linear double-stranded dna genome. it infects acidianus, which is a hyperthermostable archaea growing at 85 degrees c and acidic phs, below ph 3. afv1-99, a protein of 99 amino acids of unknown function, has homologues in the archaeal virus families lipothrixviridae and rudiviridae. we determined the crystal structure of afv1-99 at 2.05 a resolution. afv1-99 ... | 2009 | 19472363 |
| crystal structure of afv1-102, a protein from the acidianus filamentous virus 1. | viruses infecting hyperthermophilic archaea have intriguing morphologies and genomic properties. the vast majority of their genes do not have homologs other than in other hyperthermophilic viruses, and the biology of these viruses is poorly understood. as part of a structural genomics project on the proteins of these viruses, we present here the structure of a 102 amino acid protein from acidianus filamentous virus 1 (afv1-102). the structure shows that it is made of two identical motifs that ha ... | 2009 | 19319936 |