detection of myxobolus rotundus (myxozoa: myxosporea) in skin mucus of crucian carp carassius auratus auratus using a monoclonal antibody.diagnosis of myxosporean myxobolus rotundus infection was conducted by examining skin mucus from the infected crucian carp carassius auratus auratus with a monoclonal antibody, mab 2d12, raised previously against the parasite. a positive reaction was observed in skin mucus collected from infected fish, and spores and pre-spore stages of the parasite were identified by the mab 2d12. it was also demonstrated that m. rotundus infection can be successfully detected by a simple method, enzyme-linked ...200312747643
a combined phage display scfv library against myxobolus rotundus infecting crucian carp, carassius auratus auratus (l.), in china.immunological methods have been developed for the diagnosis of myxobolus rotundus but their use has been limited for the prevention and therapy of this serious parasitic pathogen. phage display antibody libraries are a powerful technique for the development of antibodies to molecules of interest and have advantages over traditional hybridroma approaches. in the present study, four antigen fractions related to m. rotundus were prepared and a combined phage display single-chain antibody fragments ...200616351693
some remarks on the occurrence, host-specificity and validity of myxobolus rotundus nemeczek, 1911 (myxozoa: myxosporea).myxobolus rotundus nemeczek, 1911 is a common and specific parasite of the common bream abramis brama (l.). small, round or ellipsoidal shaped plasmodia of this species develop in the gill and exhibit strong histotropism to the secondary gill lamellae with plasmodial development in their capillary network. m. rotundus is frequently found in mixed infection with m. bramae reuss, 1906, a parasite of the afferent arteries of gill filaments. the round spores of m. rotundus resemble several other myx ...200919048408
complete life cycle of myxobolus rotundus (myxosporea: myxobolidae), a gill myxozoan of common bream abramis brama.the life cycle of myxobolus rotundus nemeczek, 1911, a myxosporean parasite of the gills of common bream abramis brama l., was studied under laboratory conditions. mature myxobolus spp. spores from plasmodia in the gills of wild bream were used to infect naïve oligochaete worms in a flow-through system of aquaria. triactinomyxon-type actinospores were released from the oligochaetes 1 yr later and allowed to continually flow into a tank containing uninfected bream fry. the gills of the fry were c ...200919694174
some additional data to the occurrence, morphology and validity of myxobolus turpisrotundus zhang, 2009 (myxozoa: myxosporea).myxobolus turpisrotundus zhang, 2009, infects allogynogenetic gibel carp carassius auratus gibelio (bloch) and is always regarded as synonymous with myxobolus rotundus nemeczek, 1911, since its first report in goldfish carassius auratus auratus (l.) in china in 1955. in this study, it was comprehensively examined by morphological and molecular biological methods. the round spores of m. turpisrotundus are similar to those of m. rotundus from common bream abramis brama (l.) in morphology; however, ...201020352450
infection of myxobolus turpisrotundus sp. n. in allogynogenetic gibel carp, carassius auratus gibelio (bloch), with revision of myxobolus rotundus (s. l.) nemeczek reported from c. auratus auratus (l.).infection of a myxobolus species, previously identified as myxobolus rotundus, was detected in 182 of 7892 (2.31%) allogynogenetic gibel carp, carassius auratus gibelio, in a closed pond culture system in china. morphological and molecular data showed that this myxosporean is a different species from m. rotundus parasitizing abramis brama in europe and is thus designated as a new species, myxobolus turpisrotundus. m. rotundus (s.l.) ex c. auratusauratus is a synonym of m. turpisrotundus. plasmod ...201020487139
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