mitogenome assembly from genomic multiplex libraries: comparison of strategies and novel mitogenomes for five species of frogs. | next-generation sequencing continues to revolutionize biodiversity studies by generating unprecedented amounts of dna sequence data for comparative genomic analysis. however, these data are produced as millions or billions of short reads of variable quality that cannot be directly applied in comparative analyses, creating a demand for methods to facilitate assembly. we optimized an in silico strategy to efficiently reconstruct high-quality mitochondrial genomes directly from genomic reads. we te ... | 2016 | 26607054 |