Publications

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changes in the composition of the polar fraction of persian lime (citrus latifolia) during fruit growth by lc-qtof ms/ms analysis.citrus possess a large number of bioactive compounds mainly studied in ripe fruits. few studies have focused on evolution of metabolites during fruit growth. in this study, fruits were sampled from weeks 1-14 of the ripening process. polar extracts were obtained from all collected samples and analysed by liquid chromatography-tandem mass spectrometry. analysis of variance applied to the dataset indicated that the relative concentration of 394 out of 423 molecular entities changed significantly d ...028551235
characterization of water stress and low temperature effects on flower induction in citrus.experiments were conducted with containerized ;tahiti' lime (citrus latifolia tan.) trees in order to define conditions needed to induce flowering. cyclical or continuous water stress for 4 to 5 weeks induced flowering. moderate (-2.25 megapascals, midday) or severe (-3.5 megapascals, midday) water stress as measured by leaf xylem pressure potential, for as little as 2 weeks induced flowering, but the response was more significant in severely stressed trees. low temperature (18 degrees c day/10 ...198616664786
[nitrogen composition of the fruit juice of the persian lime in cuba].in 1987 to 1989 the composition was studied of the nitrogen compounds (nitrogen and free amino acids) in the fruit juice of persian lime during its commercial harvesting period. adult persian lime trees grafted on citrus macrophylla and c. volkameriana were used, planted on a groundwater-affected red ferrilytic soil in the la habana province. determination of the total nitrogen and of the free proline was carried out in may to july of each experimental year. the amino acid spectrum was establish ...19911823779
phototoxic coumarins in limes.coumarins in the rind and pulp of persian and key limes were quantified. in the rind of persian limes, coumarin concentrations were in the order: limettin > bergapten > isopimpinellin > xanthotoxin > psoralen. in the rind of key limes, psoralen and xanthotoxin were analytically absent; limettin was 10 times more concentrated than either bergapten or isopimpinellin, which were equal in concentration. coumarin content in persian lime pulp was in the order: isopimpinellin > limettin > bergapten > x ...19938505017
hot water treatment and insecticidal coatings for disinfesting limes of mealybugs (homoptera: pseudococcidae).hot water immersion and insecticidal coatings were tested to determine if they could be used to disinfest persian limes, citrus latifolia tanaka, of the mealybug pests planococcus citri risso and pseudococcus odermatti miller & williams. a 20-min 49 degrees c hot water immersion treatment is effective in killing mealybugs and all other arthropods tested found externally on limes, or under the calyx. no insects or mites were found to survive after the 20-min hot water treatment. in this test, 7,2 ...200010902365
radical-scavenging activities of citrus essential oils and their components: detection using 1,1-diphenyl-2-picrylhydrazyl.thirty-four kinds of citrus essential oils and their components were investigated for radical-scavenging activities by the hplc method using 1,1-diphenyl-2-picrylhydrazyl (dpph). to examine the oils' relative radical-scavenging activities compared with that of a standard antioxidant, trolox was employed. all of the essential oils were found to have scavenging effects on dpph in the range of 17. 7-64.0%. the radical-scavenging activities of 31 kinds of citrus essential oils were comparable with o ...200010995330
phytophagous mite populations on tahiti lime, citrus latifolia, under induced drought conditions.in the north-western region of venezuela, phyllocoptruta oleivora, tetranychus mexicanus and brevipalpus phoenicis are common plant-feeding mites on leaves, fruits and branches of tahiti lime, citrus latifolia. the population dynamics of these herbivores are affected by many factors, such as weekly treatments with wettable sulphur, particularly during the wet season, maintenance pruning of plants, irrigation with microsprinklers, induction of water stress by withholding irrigation and biotic and ...200011354617
evaluation of filamentous fungi and inducers for the production of endo-polygalacturonase by solid state fermentation.aspergillus oryzae cct 3940, aspergillus awamori nrrl 3112 and a trichoderma sp.) were compared for their capacity to produce endo-polygalacturonase (endo-pg) in solid state fermentation. maximum pectinolytic activity was reached in 72 h of growth, the best two fungal strains being a. niger t0005007-2 and a. oryzae cct 3940. three types of commercial purified pectin and four of unprocessed pectin (tangerine, orange, tahiti lime and sweet lime rind) were used to assess the effect of pectin on the ...200212240994
disease development and symptom expression of xanthomonas axonopodis pv. citri in various citrus plant tissues.abstract experimental inoculations of xanthomonas axonopodis pv. citri in different tissues of tahiti lime and pineapple sweet orange were conducted monthly under natural conditions on réunion island. the interactions between a set of environmental and epidemic variables associated with disease expression and 184 different factor combinations were investigated to determine the parameters needed to explain asiatic citrus canker (acc) disease expression. area under the disease progress curve (audp ...200318943164
nonvolatiles of commercial lime and grapefruit oils separated by high-speed countercurrent chromatography.the nonvolatile fractions of cold-pressed peel oils of key and persian lime as well as grapefruit were separated by high-speed countercurrent chromatography (hs-ccc). in addition to the isolation of the main coumarins, psoralens and polymethoxyflavones, a number of minor constituents were enriched and successfully characterized by gc-ms and hplc-uv. 5,7,8-trimethoxycoumarin and the cyclical acetals of oxypeucedanin hydrate with citral were determined as new nonvolatile trace constituents of lime ...200616536603
citrus viroid v: occurrence, host range, diagnosis, and identification of new variants.the recently described citrus viroid v (cvd-v) has been proposed as a new species of the genus apscaviroid within the family pospiviroidae. analysis of 64 samples from different citrus-growing areas has shown that cvd-v is present in the united states, spain, nepal, and the sultanate of oman. cvd-v found in six sweet orange sources from the sultanate of oman was identical to the reference cvd-v variant, whereas three new variants with sequence identities of 98.6% (cvd-vca), 97.3% (cvd-vst), and ...200818943408
a novel hybridization approach for detection of citrus viroids.citrus plants are natural hosts of several viroid species all belonging to the family pospiviroidae. previous attempts to detect viroids from field-grown species and cultivars yielded erratic results unless analyses were performed using etrog citron a secondary bio-amplification host. to overcome the use of etrog citron a number of rt-pcr approaches have been proposed with different degrees of success. here we report the suitability of an easy to handle northern hybridization protocol for viroid ...200919162174
multidimensional enantio gas chromtography/mass spectrometry and gas chromatography-combustion-isotopic ratio mass spectrometry for the authenticity assessment of lime essential oils (c. aurantifolia swingle and c. latifolia tanaka).this article focuses on the genuineness assessment of lime oils (citrus aurantifolia swingle and c. latifolia tanaka), by multi dimensional gas chromatography (mdgc) to determine the enantiomeric distribution of α-thujene, camphene, β-pinene, sabinene, α-phellandrene, β-phellandrene, limonene, linalool, terpinen-4-ol, α-terpineol and by gas chromatography-combustion isotope ratio mass spectrometry (gc-c-irms) to determine the isotopic ratios of α-pinene, β-pinene, limonene, α-terpineol, neral, g ...201122088669
antioxidant capacity and mineral content of pulp and peel from commercial cultivars of citrus from brazil.four citrus species (c. sinensis, cvs. pera and lima; c. latifolia tanaka cv. tahiti; c. limettioides tanaka cv. sweet lime and c. reticulate, cv. ponkan) grown in brazil were characterised in relation to contents of minerals, ascorbic acid, total polyphenols and antioxidant capacity of pulps and peels. in general, the peels demonstrated significantly higher contents of all compounds than the pulps (p<0.05), with the exception of the pera orange pulp that presented the highest acid ascorbic cont ...201223442635
evaluation of anti-inflammatory activity of citrus latifolia tanaka essential oil and limonene in experimental mouse models.the genus citrus (rutaceae) includes several species of plants that produce some of the most cultivated fruits in the world, providing an appreciable content of essential oil. in folk medicine, they are used as a cholagogue, antipyretic, anti-inflammatory, sedative, and antitoxic effects. lemon essential oil has been used since ancient times for its antiseptic, carminative, diuretic, and eupeptic effects. in this study, we investigated the anti-inflammatory activity of citrus latifolia tanaka es ...201323762165
the bud midge prodiplosis longifila: damage characteristics, potential distribution and presence on a new crop host in colombia.the dipteran prodiplosis longifila is a severe pest, mainly of solanaceae, in south america and some years ago it damaged tahiti lime crops in the united states. it is a potential invasive pest. despite its presence in colombia, nothing is known regarding the taxonomic identification of p. longifila or the characteristics of the damage it produces. moreover, the current and potential distributions of this pest are unknown. to determine these factors, p. longifila was sampled in several solanacea ...201525977894
determination of the host status of the 'persian' lime (citrus latifolia tanaka) for anastrepha ludens (loew) (diptera: tephritidae).field and laboratory no-choice oviposition tests were performed to determine whether the 'persian' lime (citrus latifolia tanaka) is a host of anastrepha ludens loew (diptera: tephritidae). trapping and fruit sampling were performed to determine adult population densities and the level of infestation in the two lime orchards. additionally, unharvested and harvested limes were exposed to sexually mature flies and the number of eggs laid and the immature developmental rates were determined. as a c ...201526470106
antimycotic activity and genotoxic evaluation of citrus sinensis and citrus latifolia essential oils.the aim of this study was to evaluate the antifungal activity of essential oils (eos) of citrus sinensis (c. sinensis) and citrus latifolia (c. latifolia) against five candida species: candida albicans, candida tropicalis, candida glabrata, candida lusitaniae and candida guilliermondii; and perform its genotoxic evaluation. the eos of c. sinensis and c. latifolia were obtained from the peel by hydro-distillation. the major components determined by gc-ms were in c. sinensis, d-limonene (96%) and ...201627137128
raman spectroscopy an option for the early detection of citrus huanglongbing.this research describes the application of portable field raman spectroscopy combined with a statistical analysis of the resulting spectra, employing principal component analysis (pca) and linear discriminant analysis (lda), in which we determine that this method provides a high degree of reliability in the early detection of huanglongbing (hlb) on sweet orange, disease caused by the bacteria candidatus liberibacter asiaticus. symptomatic and asymptomatic plant samples of sweet orange (citrus si ...201626988658
growth modeling of aspergillus niger strains isolated from citrus fruit as a function of temperature on a synthetic medium from lime (citrus latifolia t.) pericarp.molds are responsible for postharvest spoilage of citrus fruits. the objective of this study was to evaluate the effect of temperature on growth rate and the time to visible growth of aspergillus niger strains isolated from citrus fruits. the growth of these strains was studied on agar lime medium (al) at different temperatures, and growth rate was estimated using the baranyi and roberts model (int. j. food microbiol. 23:277-294, 1994). the rosso et al. cardinal model with inflexion (l. rosso, j ...201728574305
origin of c. latifolia and c. aurantiifolia triploid limes: the preferential disomic inheritance of doubled-diploid 'mexican' lime is consistent with an interploid hybridization hypothesis.two main types of triploid limes are produced worldwide. the 'tahiti' lime type (citrus latifolia) is predominant, while the 'tanepao' type (c. aurantiifolia) is produced to a lesser extent. both types result from natural interspecific hybridization involving a diploid gamete of c. aurantiifolia 'mexican' lime type (itself a direct interspecific c. micrantha × c. medica hybrid). the meiotic behaviour of a doubled-diploid 'mexican' lime, the interspecific micrantha/medica recombination and the re ...201729293884
antimutagenic and antioxidant activity of the essential oils of citrus sinensis and citrus latifolia.the essential oils of citrus sinensis and citrus latifolia showed antimycotic activity against candida spp. isolated from the oral cavity; they are neither mutagenic on the ames test nor cytotoxic. their main components are r-(+)-limonene, β-thujene, α-myrcene and γ-terpinene. the aim of this work was to evaluate their antimutagenic and antioxidant capacities. antimutagenic properties were evaluated against mnng and enng on s. typhimurium ta100; against 2aa on strain ta98 and in front of 4nqo an ...201728904369
pro-106-ser mutation and epsps overexpression acting together simultaneously in glyphosate-resistant goosegrass (eleusine indica).glyphosate has been used for more than 15 years for weed management in citrus groves in the gulf of mexico, at up to 3-4 applications per year. goosegrass (eleusine indica (l.) gaertn.) control has sometimes failed. in this research, the mechanisms governing three goosegrass biotypes (ein-or from an orange grove, and ein-pl1 and ein-pl2 from persian lime groves) with suspected resistance to glyphosate were characterized and compared to a susceptible biotype (ein-s). dose-response and shikimate a ...201728751654
mitochondrial dna suggests cryptic speciation in prodiplosis longifila gagné (diptera: cecidomyiidae) associated with geographic distance and host specialization.prodiplosis longifila is reported as a pest of a wide range of species cultivated in america, including citrus, solanaceous species and asparagus. this species has different behavioural traits that are primarily centred on the oviposition habit and the feeding of larvae, which can change depending on the host. however, scarce information is available on population studies and the natural history of this insect, and uncertainty exists about the taxonomic identity and the geographic distribution o ...201829345218
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