| tuberculosis in seals caused by a novel member of the mycobacterium tuberculosis complex: mycobacterium pinnipedii sp. nov. | a comparison of mycobacterium tuberculosis complex isolates from seals (pinnipeds) in australia, argentina, uruguay, great britain and new zealand was undertaken to determine their relationships to each other and their taxonomic position within the complex. isolates from 30 cases of tuberculosis in six species of pinniped and seven related isolates were compared to representative and standard strains of the m. tuberculosis complex. the seal isolates could be distinguished from other members of t ... | 2003 | 13130011 |
| identification of genetic markers for mycobacterium pinnipedii through genome analysis. | tuberculosis in seals is caused by mycobacterium pinnipedii, a member of the mycobacterium tuberculosis complex. in this study, we evaluated the extent of genetic variability among mycobacterium bovis and m. pinnipedii by microarray-based comparative genomics. we identified two deletions that are exclusive to m. pinnipedii: pid1 that removes the orthologues of the m. tuberculosis genes rv3530c and rv3531c, and pid2 that encompasses genes rv1977 and rv1978. interestingly, a deletion overlapping t ... | 2005 | 15979818 |
| study of the gyrb gene polymorphism as a tool to differentiate among mycobacterium tuberculosis complex subspecies further underlines the older evolutionary age of 'mycobacterium canettii'. | the present investigation evaluated the pcr-restriction fragment length polymorphism (rflp) analysis of hsp65 and gyrb targets for differentiation of the species within the mycobacterium tuberculosis complex (mtc) both by including new restriction enzymes and previously unstudied species. the hsp65 restriction analysis using hhai resulted in a characteristic 'mycobacterium canettii' pattern. a study of the gyrb gene polymorphism using taqialpha and hinfi allowed the initial division of mtc into ... | 2006 | 16517119 |
| novel genetic polymorphisms that further delineate the phylogeny of the mycobacterium tuberculosis complex. | in a previous report, we described a pcr protocol for the differentiation of the various species of the mycobacterium tuberculosis complex (mtc) on the basis of genomic deletions (r. c. huard, l. c. de oliveira lazzarini, w. r. butler, d. van soolingen, and j. l. ho, j. clin. microbiol. 41:1637-1650, 2003). that report also provided a broad cross-comparison of several previously identified, phylogenetically relevant, long-sequence and single-nucleotide polymorphisms (lsps and snps, respectively) ... | 2006 | 16740934 |
| mycobacterium pinnipedii: transmission from south american sea lion (otaria byronia) to bactrian camel (camelus bactrianus bactrianus) and malayan tapirs (tapirus indicus). | tuberculosis infections caused by mycobacterium (m.) pinnipedii in a south american sea lion, bactrian camel, and malayan tapirs kept in two zoological gardens spanning a time period of 5 years are reported. the zoos were linked by the transfer of one tapir. conventional bacteriological and molecular methods were applied to detect the pathogen. spoligotyping and miru/vntr-typing performed to assess the genetic similarity revealed identical molecular characteristics of the isolates from all anima ... | 2008 | 17913401 |
| deciphering the genetic bases of the structural diversity of phenolic glycolipids in strains of the mycobacterium tuberculosis complex. | phenolic glycolipids (pgl) play a major role in the virulence of mycobacteria, notably in strains of the mycobacterium tuberculosis complex and in mycobacterium leprae. the structure of the carbohydrate domain of these compounds is highly variable, and the genetic bases for these variations remain unknown. we demonstrated that the monoglycosylated pgl formed by mycobacterium bovis differs from the triglycosylated pgl synthesized by m. tuberculosis (pgl-tb) because of the following two genetic de ... | 2008 | 18390543 |
| transmission of mycobacterium pinnipedii to humans in a zoo with marine mammals. | an outbreak of tuberculosis (tb) in sea lions occurred recently in a zoo in the netherlands. the disease was detected in a captive colony consisting of 29 animals kept in an open air basin with an indoor night house. approximately 25 animal keepers were in close contact with the animals. | 2008 | 19017459 |
| [differentiation of species within the mycobacterium tuberculosis complex by molecular techniques]. | the mycobacterium tuberculosis complex includes the following species: mycobacterium tuberculosis, mycobacterium africanum, mycobacterium bovis, mycobacterium bovis-bcg, mycobacterium microti, mycobacterium caprae, mycobacterium pinnipedii, and mycobacterium canettii. these species cause tuberculosis in humans and animals. identification of mycobacterial strains has classically been performed by phenotype study. over the last years, laboratories have developed several molecular techniques to dif ... | 2009 | 19409673 |
| line probe assay for differentiation within mycobacterium tuberculosis complex. evaluation on clinical specimens and isolates including mycobacterium pinnipedii. | a line probe assay (genotype mtbc) was evaluated for species differentiation within the mycobacterium tuberculosis complex (mtbc). we included 387 mtbc isolates, 43 is6110 low-copy mtbc isolates, 28 clinical specimens with varying microscopy grade, and 30 isolates of non-tuberculous mycobacteria. the assay was 100% specific and identified all 387 isolates and 98% of all is6110 low-copy strains in concordance with the gold standard. the 2% discrepancy was caused by 1 isolate showing a faint restr ... | 2009 | 20001279 |
| computed tomographic examination of south american sea lions (otaria flavescens) with suspected mycobacterium pinnipedii infection. | ten south american sea lions (otaria flavescens) were presented for clinical evaluation and diagnosis of tuberculosis following known exposure to mycobacterium pinnipedii. ct was used to determine whether foci of calcification in mediastinal lymph nodes, typically associated with pinniped tuberculosis, could be detected and whether ct was a useful diagnostic modality, in conjunction with other tests, for the diagnosis of tuberculosis in this species. blood was collected from the caudal gluteal v ... | 2011 | 21984562 |
| naming spoligotype patterns for the rd9-deleted lineage of the mycobacterium tuberculosis complex; www.mbovis.org. | this www.mbovis.org website and associated databases were initiated in response to the need of the mycobacterium bovis molecular typing community for standardisation of nomenclature for spoligotype patterns. the purpose of the website is to provide standardised names for spoligotype patterns from strains of the rd9-deleted, lineage of the mycobacterium tuberculosis complex. each unique spoligotype pattern is named by 'sb' followed by a four integer number (e.g. sb0120) and the database currently ... | 2011 | 21855653 |
| pinniped tuberculosis in malayan tapirs (tapirus indicus) and its transmission to other terrestrial mammals. | in the last 7 yr, three different species of terrestrial mammals were diagnosed with mycobacterium pinnipedii either within one collection or through the introduction of an infected animal from another zoo. the affected species included the malayan tapir (tapirus indicus), bactrian camel (camelus bactrianus bactrianus), and crested porcupine (hystrix cristata). in the first zoo, all of these were living in exhibits adjacent to a group of south american sea lions (otariaflavescens) and were cared ... | 2011 | 22946398 |
| phylogenetic polymorphisms in antibiotic resistance genes of the mycobacterium tuberculosis complex. | sequence analysis of known antibiotic resistance genes of the mycobacterium tuberculosis complex (mtbc) is increasingly being used to infer phenotypic resistance to a variety of antibiotics. however, a clear understanding of the genotype-phenotype relationship is required to interpret genotypic susceptibility results accurately. in this context, it is particularly important to distinguish phylogenetically informative neutral polymorphisms from true resistance-conferring mutations. | 2014 | 24458512 |
| multidrug-resistant mycobacterium tuberculosis of the latin american mediterranean lineage, wrongly identified as mycobacterium pinnipedii (spoligotype international type 863 [sit863]), causing active tuberculosis in south brazil. | we recently detected the spoligotype patterns of strains of mycobacterium pinnipedii, a species of the mycobacterium tuberculosis complex, in sputum samples from nine cases with pulmonary tuberculosis residing in porto alegre, south brazil. because this species is rarely encountered in humans, we further characterized these nine isolates by additional genotyping techniques, including 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (miru-vntr) typing, verificatio ... | 2015 | 26400784 |
| mycobacterium pinnipedii tuberculosis in a free-ranging australian fur seal (arctocephalus pusillus doriferus) in south australia. | this report describes the first case in south australia, australia, of mycobacterium pinnipedii tuberculosis in a free-ranging australian fur seal (arctocephalus pusillus doriferus). severe pyogranulomatous pleuropneumonia with intrahistocytic acid-fast beaded filamentous bacilli was seen on histology. m. pinnipedii was confirmed by full 24-loci mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (miru-vntr) typing. spillover concerns for public health and cattle are discuss ... | 2014 | 25632695 |
| mycobacterium pinnipedii in a stranded south american sea lion (otaria byronia) in brazil. | we report tuberculosis in a stranded south american sea lion (otaria byronia) in brazil caused by mycobacterium pinnipedii, a member of mycobacterium tuberculosis complex. | 2014 | 24484491 |
| the seal tuberculosis agent, mycobacterium pinnipedii, infects domestic cattle in new zealand: epidemiologic factors and dna strain typing. | the fur seal (arctocephalus forsteri), which is abundant in coastal areas of new zealand, harbors several zoonotic pathogens, including mycobacterium pinnipedii, a member of the mycobacterium tuberculosis complex. we describe the microbiology and epidemiology of seven cases of m. pinnipedii infection in beef cattle (bos primigenius) in coastal areas of new zealand in 1991-2011. epidemiologic factors were analyzed on six case farms and a telephone survey of 55 neighboring farms. a dna-strain typi ... | 2014 | 24484478 |