cross-reactive and type-specific complement-fixing structures of oriboca virions.characteristics of two components of oriboca virus were studied after they were separated and semipurified by velocity centrifugation. a component of low infectivity and broad cross-reactivity in the complement-fixation (cf) test had a sedimentation coefficient of 6--7s. infectious oriboca virions with hemagglutinating (ha) activity had a sedimentation coeffecient of 457s. these virions cross-reacted broadly with murutucu viral antibody. the cross-reactive, virion-associated component and a type ...197990121
specific immunity and nonspecific resistance to infection: listeria, protozoa, and viruses in mice and hamsters,.specific immunity developed by mice against protozoan (toxoplasma gondii and besnoitia jellisoni) and bacterial (listeria monocytogenes) infections was compared with nonspecific protection conferred by prior infections. the results indicated that homologous immunity protected mice from more than 10-5 ld50 of t. gondii or b. jellisoni, but from only 10-2 ld50 of l. monocytogenes. heterospecific protection among these organisms was for 10-0.4 minus 10-1.2 ld50. in studies in hamsters specific immu ...1975165241
[arboviruses. xvi--research on oriboca virus]. 1979318084
[occurrence of arboviruses in the state of rio de janeiro. incidence of antibody inhibitors of hemagglutination against oriboca virus (arbovirus group c)]. 19744549847
physical properties and antigenic components of oriboca virus.two complement fixation (cf) components were detected in all of the various preparations (mouse liver, brain, serum, and bhk-21 cell culture) of oriboca examined by equilibrium density centrifugation. the cf components possessed a buoyant density of 1.21 and 1.18 g/ml, and they were precipitated by treatment with protamine. analysis of oriboca virus by rate zonal sedimentation separated three components. the most rapidly sedimenting component was the infectivity which cosedimented with a peak of ...19734736995
structural components of oriboca virus.analysis of purified oriboca virions by neutral, sodium dodecyl sulfate polyacrylamide-gel electrophoresis indicated the presence of three structural polypeptides designated v-1, v-2, and v-3 on the basis of their relative electrophoretic mobilities in 8% gels. polypeptides v-2 and v-3 are glycopeptides associated with the virion envelope as demonstrated by the preferential incorporation of labeled glucosamine into the polypeptides and by release of the polypeptides from the intact virion by the ...19744821489
in vivo definition of the functional origin of replication (ori(+)) of the promiscuous plasmid pls1.we have defined the minimal origin of replication of the plasmid pls1 leading strand, as comprised within a 247 bp region, by in vivo deletion analyses. cloning of pls1 dna regions containing its oriv(+) into a compatible replicon resulted in weak incompatibility towards pls1, but only when the cloned fragment included the entire pls1 oriv(+). plasmids lacking a functional repb gene (which encodes the pls1 initiator of replication repb protein) could be established in streptococcus pneumoniae on ...19938455568
molecular epidemiology of group c viruses (bunyaviridae, orthobunyavirus) isolated in the date, no molecular studies on group c viruses (bunyaviridae, orthobunyavirus) have been published. we determined the complete small rna (srna) segment and partial medium rna segment nucleotide sequences for 13 group c members. the full-length srna sequences ranged from 915 to 926 nucleotides in length, and revealed similar organization in comparison with other orthobunyaviruses. based on the 705 nucleotides of the n gene, group c members were distributed into three major phylogenetic groups, ...200516051848
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