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small intestinal cytochromes p450.small intestinal cytochromes p450 (p450) provide the principal, initial source of biotransformation of ingested xenobiotics. the consequences of such biotransformation are detoxification by facilitating excretion, or toxification by bioactivation. p450s occur at highest concentrations in the duodenum, near the pylorus, and at decreasing concentrations distally--being lowest in the ileum. highest concentrations occur from midvillus to villous tip, with little or none occurring in the crypts of li ...19911801846
glutathione s-transferase subunit induction patterns of brussels sprouts, allyl isothiocyanate and goitrin in rat liver and small intestinal mucosa: a new approach for the identification of inducing xenobiotics.effects of brussels sprouts (2.5-30%), allyl isothiocyanate (0.03 and 0.1%) and goitrin (0.02%), in the diet, on the glutathione s-transferase subunit pattern in the liver and small intestinal mucosa of male fisher rats were investigated. a statistically significant linear relationship was found between the amount of brussels sprouts in the diet and the induction of glutathione s-transferase subunits in two experiments. increases in total activity of glutathione s-transferases towards 1-chloro-2 ...19902341092
effects of dietary schizandra chinensis, brussels sprouts and illicium verum extracts on carcinogen metabolism systems in mouse liver.ethanol extracts of brussels sprouts (brx), schizandra chinensis (szx) or illicium verum (ivx) were added to a semi-purified basal diet and fed to adult male and female c57b1/6 mice for 14 and 10 days, respectively. other groups received the unsupplemented basal diet or a mouse chow. liver fractions were prepared from these mice to investigate the effects of the diets on the enzyme systems involved in benzo[a]pyrene (bp) and aflatoxin b1 (afb1) metabolism. the effects of the microsomal or cytoso ...19863096852
the effects of dietary brussels sprouts and schizandra chinensis on the xenobiotic-metabolizing enzymes of the rat small intestine.after an initial equilibration period of 7 days on a semi-synthetic basal diet, male sprague-dawley rats were fed for 2 wk on either the basal diet (controls), the basal diet containing 5% schizandra chinensis or 25% brussels sprouts, or on rat chow. one group of chow-fed rats was pretreated with 20 mg 3-methylcholanthrene (3-mc)/kg body weight, 24 hr before they were killed. microsomal and cytosolic fractions were prepared from small intestine mucosa. microsomes were assayed for cytochrome p-45 ...19853871719
improved enrichment for recovery of shigella sonnei from foods.shigella species were recovered from foods by the procedure described in the bacteriological analytical manual, 5th ed. the method is effective if shigella species are present at about 10(6) cells/g. a 25 g food portion was incubated in gram-negative (gn) and selenite cystine broths for 16 h at 35 degrees c and streaked onto macconkey, levine's eosin methylene blue, desoxycholate citrate, and xylose lysine desoxycholate agars. s. sonnei cells were recovered quantitatively at 44.5 degrees c, and ...19853894320
effects of dietary cabbage, brussels sprouts, illicium verum, schizandra chinensis and alfalfa on the benzo[alpha]pyrene metabolic system in mouse liver.male c57b16 mice were fed on diets containing either 20% cabbage, 20% brussels sprouts, 20% alfalfa, 5% schizandra chinensis or 5% illicium verum (two chinese medicinal herbs) or on a chow or purified basal diet for 14 days after a 1-wk equilibration period on the basal diet. liver microsomal fractions were assayed for cytochrome p-450 content, aryl hydrocarbon hydroxylase (ahh) and epoxide hydrolase (eh). liver microsome-mediated benzo[a]pyrene (bp) metabolism (with and without an eh inhibitor, ...19836311701
comparison of the effects of brussels sprouts, glucosinolates, and glucosinolate metabolite consumption on rat hepatic polysubstrate monooxygenases. 19836677468
effect of dietary indole-3-carbinol on intestinal and hepatic monooxygenase, glutathione s-transferase and epoxide hydrolase activities in the rat.male sprague-dawley rats were fed on purified diets supplemented with 50-500 ppm indole-3-carbinol (i3c), a compound present in cruciferous vegetables, or with 25% brussels sprouts (brassica oleracea) for 10 days after a 1-wk equilibration on a purified diet. cytosolic and microsomal fractions were prepared from liver and intestinal mucosae. intestinal aryl hydrocarbon hydroxylase (ahh) activity was increased significantly (p less than 0.05) over basal levels by i3c at 50 ppm (a 6.1-fold increas ...19846334634
glutathione s-transferase activity: enhancement by compounds inhibiting chemical carcinogenesis and by dietary constituents.benzyl isothiocyanate, beta-naphthoflavone, coumarin, alpha-angelicalactone, disulfiram, indole-3-carbinol and indole-3-acetonitrile induced increased glutathione (gsh) s-transferase activity in the liver and small intestine in female icr/ha mice. all seven compounds are inhibitors of chemical carcinogenesis. in additional work, several dietary constituents increased gsh s-transferase activity. consumption of diets containing dried powdered preparations of brussels sprouts, cabbage, coffee beans ...19826278195
effects of commonly consumed vegetables on hepatic xenobiotic-metabolizing enzymes in the mouse.to investigate the effects of consuming a variety of common vegetables on the activities of xenobiotic-metabolizing enzymes in the mouse liver, male c57bl/6 mice were fed purified diets supplemented isocalorically with 20% freeze-dried powdered kidney bean, soya bean, alfalfa, cauliflower, mustard greens, carrot, kale, brussels sprouts, beet, egg plant or onion or with 40% sweet potato. hepatic ethoxycoumarin o-deethylase (ecd) activity was increased significantly (p less than or equal to 0.05) ...19853877667
diffuse storage of vegetal wax hydrocarbons of dietary origin. pathologic and chemical findings in a case.a 55-year-old man died suddenly, after presenting with unstable angina and an asymptomatic micronodular pulmonary pattern. autopsy revealed storage of a crystallized fatty substance in the lymph nodes, liver, spleen, adrenal glands, and lungs. in the latter organ, the deposition formed foreign body granulomas, accounting for the radiographic appearance. mass spectrometry identified the fatty substance as straight-chain saturated hydrocarbons (n-alkanes) of carbon-29 and carbon-31 atoms, which ar ...19892705874
effect of dietary brussels sprouts with increased selenium content on mammary carcinogenesis in the rat.brussels sprouts (brassica oleracea, l; jade cross e, hybrid cultivar) were cultivated with inorganic selenium added to the plant growth medium. sprague-dawley, female, weanling rats were divided into groups and fed 20% brussels sprouts diets containing either 0.03, 0.58, 1.29, or 6.71 ppm of selenium naturally occurring in the sprouts. these diets were fed 2 weeks before and 2 weeks after a single dose of 7,12-dimethylbenz[a]anthracene (dmba), and the rats were then placed on a low selenium bas ...19892496915
the effect of feeding brassica vegetables and intact glucosinolates on mixed-function-oxidase activity in the livers and intestines of rats.significant induction of mixed-function-oxidase (mfo) activity was observed in the small intestines of rats within 4-6 hr of ingestion of a single meal containing a brassica vegetable (cabbage). intact brussels sprouts and a fractionated methanol-water extract of brussels sprouts induced similar degrees of mfo activity in the livers, and small and large intestines of rats. however, the residue left after extraction of the polar compounds did not induce mfo activity. different amounts of the vari ...19892473016
preliminary observations on the effect of dietary brussels sprouts on thyroid function.brassica vegetables are the major source of glucosinolates in the human diet. certain glucosinolates are readily converted into goitrogenic species, notably 5-vinyloxazolidine-2-thione and thiocyanate ion. the effect of dietary brussels sprouts, a particularly rich source of such glucosinolates, on thyroid function has been examined. inclusion of cooked brussels sprouts (150 g daily for 4 weeks) into a normal diet of 10 volunteer subjects had no effect on thyroid function as determined by measur ...19862419242
fermentation of vegetable fiber in the intestinal tract of rats and effects on fecal bulking and bile acid excretion.the fermentative breakdown and fecal bulking capacity of dietary fiber from various vegetables (carrots, rutabagas, peas, green beans and brussels sprouts) were evaluated in balance experiments in rats. the excretion of bile acids, protein and fat was also studied. the addition of blanched vegetables providing 10 g of fiber/100 g of diet caused a two-(rutabagas) to threefold (carrots) increase in fecal dry weight compared to that with a basal fiber-free diet. with green beans and brussels sprout ...19902160526
effects of high levels of brussels sprouts in the diet of rats.to examine the possible harmful effects of feeding brussels sprouts to rats, groups of 10 male, weanling rats received the non-dehydrated vegetable in moist diets at levels providing 2.5-30% of the dry matter for 4 wk. a first study comprised test diets with 15 and 30% of the dry matter as uncooked or cooked brussels sprouts and control diets without and with 0.2% potassium thiocyanate (kscn) for comparison. the second study comprised diets with 0, 2.5, 5, 10 and 20% of the cooked vegetable and ...19911765328
dietary constituents altering the responses to chemical carcinogens.this paper deals with two categories of compounds having the capacity to inhibit the neoplastic effects of chemical carcinogens on the host. the first are inducers of increased microsomal mixed function oxidase activity. an increasing number of these inducers are being found in natural products. cruciferous vegetables including brussels sprouts, cabbage, and cauliflower contain such compounds. recently indole-3-acetonitrile, indole-3-carbinol and 3,3'-diindolylmethane have been identified as ind ...19761261715
aryl hydrocarbon hydroxylase induction in rat tissues by naturally occurring indoles of cruciferous plants.a phytochemical investigation to identify inducers of increased aryl hydrocarbon hydroxylase (ahh) activity from three cruciferous vegetables, brussels sprouts, cabbage, and cauliflower, resulted in the identification of indole-3-acetonitrile, indole-3-carbinol, and 3,3'-diindolylmethane as naturally occurring inducers. these compounds are produced during the hydrolysis of indolyl-methyl glucosinolate by the plant enzyme myrosinase.19751127728
regulation of drug metabolism in man by environmental chemicals and diet.studies in animals have shown that many environmental pollutants induce the synthesis or inhibit the activity of microsomal mixed-function oxygenases that metabolize drugs, carcinogens and normal body constituents such as steroid hormones. these effects on microsomal enzyme activity alter the duration and intensity of action of foreign and endogenous chemicals in animals, and such effects on metabolism may influence the carcinogenicity of some pollutants in man. studies on the effects of environ ...1977844609
stimulatory effect of brussels sprouts and cabbage on human drug metabolism. 1979758247
folate conjugase activity in fresh vegetables and its effect on the determination of free folate content.the levels of naturally-occurring folate conjugates in fresh asparagus, broccoli, brussels sprouts, cabbage, cauliflower lettuce, and spinach were determined together with the effect on the hydrolysis of pteroylpolyglutamates during the extraction phase of the lactobacillus casei assay. the levels of conjugate activity vary widely among the vegetables tested, from a low of 0.30 +/- 0.21 in lettuce to a high of 106.2 +/- 6.6 mumoles/hr per 100 mg of fresh weight in spinach. the free folate values ...1979760505
aromatic hydrocarbon responsiveness-receptor agonists generated from indole-3-carbinol in vitro and in vivo: comparisons with 2,3,7,8-tetrachlorodibenzo-p-dioxin.indole-3-carbinol (i3c) is a secondary plant metabolite produced in vegetables of the brassica genus, including cabbage, cauliflower, and brussels sprouts. i3c is both an anti-initiator and a promoter of carcinogenesis. consumption of i3c by humans and rodents can lead to marked increases in activities of cytochrome p-450-dependent monooxygenases and in a variety of phase ii drug-metabolizing enzymes. we have reported previously that the enzyme-inducing activity of i3c is mediated through a mech ...19911658785
effects of cooked brussels sprouts on cytochrome p-450 profile and phase ii enzymes in liver and small intestinal mucosa of the rat.male wistar rats were given semi-synthetic diets supplemented with 0, 2.5, 5 and 20% cooked brussels sprouts for 2, 7, 14 or 28 days. the effects on several cytochrome p-450 enzymes and phase ii enzymes (glutathione s-transferase (gst), glucuronyl transferases 1 and 2 (gt1 and gt2) and dt-diaphorase (dtd)) in the liver and small intestinal mucosa were investigated. from 2 days of exposure onwards brussels sprouts induced p4501a2 and--to a lesser extent--p4501a1 apoprotein levels in the liver, wh ...19921544602
stimulatory effect of vegetables on intestinal drug metabolism in the rat.the intestinal metabolism of hexobarbital, phenacetin, 7-ethoxycoumarin and benzo [a] pyrene in vitro was increased in rats fed either dried brussels sprouts or dried cabbage in a nutritionally complete semisynthetic diet as compared to rats fed only the semisynthetic diet. pretreatment of rats with several indoles present in brussels sprouts and cabbage also stimulated intestinal drug metabolism, but the effect was smaller than when dried brussels sprouts or dried cabbage was fed. the results o ...1976948025
effect of diet and route of administration on the dna binding of aflatoxin b1 in the rat.the effects of dietary brussels sprouts and indole-3-carbinol (i3c) on xenobiotic-metabolizing enzyme activities and hepatic aflatoxin b1 (afb1)-dna binding were determined in rats. animals were dosed intraperitoneally (i.p.) or intragastrically (i.g.) with [3h]afb1 and killed 2 (i.p.) or 3 (i.g.) h later. brussels sprouts caused a significant (p less than 0.01) 50-60% decrease in hepatic afb1-dna binding, and increased hepatic and intestinal glutathione s-transferase (gst) activities. hepatic m ...19892702710
effect of diet on the hepatotoxicity of polybrominated biphenyls (firemaster pb-6).the consumption of diets formulated with cruciferae vegetables, e.g., cauliflower, cabbage, and brussels sprouts, has been shown to result in a stimulation of the intestinal and hepatic microsomal enzyme systems in rats. this study was designed to determine if this increase in intestinal and hepatic microsomal enzyme activity affected the hepatic response to polybrominated biphenyls (pbb). after three weeks of consuming either a semipurified or 25% cauliflower leaf-supplemented diet (cld), male ...1978209966
modifying actions of solvent extracts from fruit and vegetable residues on 2-amino-3-methylimidazo[4,5-f]quinoline (iq) and 2-amino-3,4-dimethylimidazo[4,5-f]quinoxaline (meiqx) induced mutagenesis in salmonella typhimurium ta 98.the edible parts of 13 fruits--apples, apricots, bananas, blackberries, sweet cherries, red currants, white grapes, oranges, peaches, pears, plums, raspberries, and strawberries--and of 12 vegetables--asparagus, green beans, broccoli, brussels sprouts, red and white cabbage, carrots, cauliflower, onions, green peppers, spinach, and tomatoes--were squeezed in order to separate juices and residues. the residues were washed, lyophilized, and extracted sequentially with n-hexane, dichloromethane, ac ...19957531289
regulation of human drug metabolism by dietary factors.several dietary factors influence the oxidative metabolism of chemicals in humans. increasing the ratio of protein to carbohydrate or fat in the diet, feeding cabbage and brussels sprouts or feeding charcoal-broiled beef for several days stimulates human drug metabolism. the chronic ingestion of ethanol stimulates drug metabolism whereas the chronic ingestion of methylxanthine-containing foods inhibits drug metabolism. in contrast, an increase in the ratio of fat to carbohydrate in the diet of n ...19806906262
in vitro antimutagenic and in vivo anticlastogenic effects of carotenoids and solvent extracts from fruits and vegetables rich in carotenoids.the water insoluble residues of some carotenoid-rich fruits and vegetables, such as apricots, oranges, brussels sprouts, carrots, yellow-red peppers, and tomatoes, were sequentially extracted with n-hexane, dichloromethane, acetone, and 2-propanol, and solvent extracted materials were tested for inhibition of mutagenicities induced by aflatoxin b1 (afb1), benzo[a]pyrene (bap), 2-amino-3-methylimidazo[4,5-f]quinoline (iq), and cyclophosphamide (cp) in histidine-deficient strains of salmonella typ ...19989639691
oltipraz: a laboratory and clinical review.oltipraz [5-(2-pyrazinyl)-4-methyl-1,2-dithiole-3-thione; rp 35972] is a synthetic, substituted 1,2-dithiole-3-thione previously used in humans as an antischistosomal agent. cruciferous vegetables (e.g., brussels sprouts, cabbage) contain several agents, including dithiolethiones, which appear to inhibit carcinogenesis; however, it is unclear which dietary compounds produce the protective effects. animal studies have demonstrated that oltipraz is a potent inducer of phase ii detoxification enzym ...19938412206
dietary influences on rat hepatic and intestinal dt-diaphorase activity.dt-diaphorase (dtd) is a flavoprotein that catalyses the two-electron reduction of various redox dyes and quinones such as menadione and phylloquinone. it has been proposed that this enzyme may have a protective effect against cancer, as the two-electron reduction prevents the formation of toxic oxygen metabolites that may be generated as a result of the one-electron reduction catalysed by enzymes such as nadph-cytochrome p-450 reductase. the effects of a purified diet supplemented with either 2 ...19863096849
hepatic glutathione s-transferase activity and aflatoxin b1-induced enzyme altered foci in rats fed fractions of brussels sprouts.the aim of the present study was to determine whether the liver cytosol detoxication enzymes, glutathione s-transferases (gstases) as well as gamma-glutamyl transpeptidase (ggt) foci induced by aflatoxin b1 (afb) were changed by feeding weanling rats diets containing brussels sprouts, a glucosinolate fraction of brussels sprouts (extract), or a non-glucosinolate fraction (residue). all 3 of these diets induced high levels of hepatic gstase specific activity as compared to purified-basal diet fed ...19852865002
effects of fertilizer on insecticides adsorption and biodegradation in crop soils.recent organic fertilizer treatments (cow manure, pig slurry, composts, or green manure) simultaneously increase insecticide adsorption onto soil and the insecticide soil persistence, indicating a mechanism of slow release of insecticide into soil by the organic matter. this occurred in sugar beet crops with aldicarb, thiofanox and imidacloprid; also, in leek, cauliflower and brussels sprouts crops with chlorpyrifos and chlorfenvinphos. in contrast, organic fertilizer treatments applied once or ...19968687996
are whole extracts and purified glucosinolates from cruciferous vegetables antioxidants?fruits and vegetables contain several classes of compounds that can potentially contribute to antioxidant activity, including vitamins, simple and complex phenolics, sulphur-containing compounds and glucosinolates. the glucosinolates are found in high concentration in many cruciferous vegetables, and it is well established that their breakdown products induce endogenous antioxidant defences such as quinone reductase and glutathione s-transferase in cells and in vivo. despite the anticarcinogenic ...19968814445
in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline.the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ...19948206443
cancer risk and oxidative dna damage in man.in living cells reactive oxygen species (ros) are formed continuously as a consequence of metabolic and other biochemical reactions as well as external factors. some ros have important physiological functions. thus, antioxidant defense systems cannot provide complete protection from noxious effects of ros. these include oxidative damage to dna, which experimental studies in animals and in vitro have suggested are an important factor in carcinogenesis. despite extensive repair oxidatively modifie ...19968862511
effect of brussels sprouts on oxidative dna-damage in man.the effect of consumption of brussels sprouts on the excretion of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) into human urine was investigated. ten non-smoking volunteers (five males and five females) were randomly assigned to two groups. five volunteers started on a diet of 300 g of glucosinolate-free vegetables whereas the other five consumed 300 g of brussels sprouts per day. after 1 week dietary regimes were reversed. levels of 8-oxodg in 24-h urine samples were measured by high-performan ...19979103270
genotoxic effects of crude juices from brassica vegetables and juices and extracts from phytopharmaceutical preparations and spices of cruciferous plants origin in bacterial and mammalian cells.crude juices of eight brassica vegetables as well as juices and extracts of spices and phytopharmaceutical preparations from cruciferous vegetables were tested for induction of point mutations in salmonella ta98 and ta100, repairable dna damage in e.coli k-12 cells and clastogenic effects in mammalian cells. in bacterial assays, all juices caused genotoxic effects in the absence of metabolic activation, the ranking order being: brussels sprouts > white cabbage > cauliflower > green cabbage > koh ...19968827059
indole-3-carbinol inhibits the expression of cyclin-dependent kinase-6 and induces a g1 cell cycle arrest of human breast cancer cells independent of estrogen receptor signaling.indole-3-carbinol (i3c), a naturally occurring component of brassica vegetables such as cabbage, broccoli, and brussels sprouts, has been shown to reduce the incidence of spontaneous and carcinogen-induced mammary tumors. treatment of cultured human mcf7 breast cancer cells with i3c reversibly suppresses the incorporation of [3h]thymidine without affecting cell viability or estrogen receptor (er) responsiveness. flow cytometry of propidium iodide-stained cells revealed that i3c induces a g1 cell ...19989461564
diet in the epidemiology of cancer of the colon and rectum.we examined the diets as reported in interviews of 256 white male patients with cancer of the colon and of 330 white male patients with cancer of the rectum. controls were 783 patients with nonneoplastic, nondigestive system diseases distributed by age similarly to the colon cancer patients and 628 patients with nonneoplastic, nondigestive diseases distributed by age like those with cancer of the rectum. we found no increase in risk for cancer of the colon or rectum regardless of the amounts of ...1978278848
a comparison of the individual and collective effects of four glucosinolate breakdown products from brussels sprouts on induction of detoxification enzymes.four glucosinolate derivatives were evaluated individually and as a mixture for their effects on hepatic p4501a (cyp1a), glutathione s-transferase (gst), quinone reductase (qr), glutathione reductase (g-rd), and gsh levels. doses of the derivatives were chosen to represent their relative abundance in brussels sprouts. adult male f344 rats received either corn oil (vehicle); one of the agents: indole-3-carbinol (i3c, 56 mg/kg), iberin (38 mg/kg), phenylethylisothiocyanate (peitc, 0.1 mg/kg), or c ...19989512722
a new method to monitor airborne inoculum of the fungal plant pathogens mycosphaerella brassicicola and botrytis cinerea.we describe a new microtiter immunospore trapping device (mtist device) that uses a suction system to directly trap air particulates by impaction in microtiter wells. this device can be used for rapid detection and immunoquantification of ascospores of mycosphaerella brassicicola and conidia of botrytis cinerea by an enzyme-linked immunosorbent assay (elisa) under controlled environmental conditions. for ascospores of m. brassicicola correlation coefficients (r(2)) of 0.943 and 0.9514 were obser ...200010877797
estrogenic effects of extracts from cabbage, fermented cabbage, and acidified brussels sprouts on growth and gene expression of estrogen-dependent human breast cancer (mcf-7) cells.cruciferous vegetable extracts from freeze-dried cabbage (fdc), freeze-dried fermented cabbage (fds), and acidified brussels sprouts (abs) were prepared by exhaustive extraction with ethyl acetate. estrogenic and antiestrogenic effects of these extracts were analyzed. to identify whether the extracts are potential estrogen receptor (er) ligands that can act as agonists or antagonists, the binding affinity of extracts for the er was measured using a competitive radiometric binding assay. the extr ...200011052710
induction of rat pancreatic glutathione s-transferase and quinone reductase activities by a mixture of glucosinolate breakdown derivatives found in brussels sprouts.the chemoprotective effects of cruciferous vegetables against cancer has been linked to the induction of detoxification enzymes, including the phase ii enzymes, glutathione s-transferases (gst) and quinone reductase (qr). four glucosinolate breakdown products found in brussels sprouts and previously shown individually to affect detoxification enzymes--(1-cyano-2-hydroxy-3-butene (crambene), indole-3-carbinol (i3c), phenylethyl isothiocyanate (peitc) and 1-isothiocyanato-3-(methylsulfinyl)-propan ...19989662411
effects of a brussels sprouts extract on oxidative dna damage and metabolising enzymes in rat liver.the apparent anticarcinogenic effect of cruciferous vegetables found in numerous epidemiological and experimental studies has been associated with their influence on phase i and phase ii metabolising enzymes as well as on the antioxidant status. in the present study we investigated the effect of administration of a brussels sprouts extract on the expression at the mrna level and/or catalytic activity in rat liver of three phase i enzymes [cytochrome p450-1a2 (cyp1a2),-2b1/2 (cyp2b1/2) and-2e1 (c ...200111346482
influence of non-nutrient plant components on biotransformation enzymes.plant foods contain numerous non-nutritive substances which exert biological activity. most attention has been focused on the anticarcinogenic effects of these compounds. many of the mechanisms involved include induction or inhibition of biotransformation enzymes. each individual has its own isoenzyme pattern for the various drug-metabolizing enzymes. the multiplicity of these enzymes results in differential responses to dietary constituents. a substance may increase the level of a certain p450, ...19979436524
selective responsiveness of human breast cancer cells to indole-3-carbinol, a chemopreventive agent.indole-3-carbinol (i3c), a compound found in cabbage, broccoli, and brussels sprouts, inhibits the growth of mammary tumors when fed to certain strains of mice. the chemopreventive and antitumor effects of i3c depend on the species and tissue type. the mechanism of action and specific human cell types that respond to i3c are not known.19948271295
prevention of oxidative dna damage in rats by brussels sprouts.the alleged cancer preventive effects of cruciferous vegetables could be related to protection from mutagenic oxidative dna damage. we have studied the effects of brussels sprouts, some non-cruciferous vegetables and isolated glucosinolates on spontaneous and induced oxidative dna damage in terms of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) in groups of 6-8 male wistar rats. excess oxidative dna damage was induced by 2-nitropropane (2-np 100 mg/kg). four days oral administration of 3 g of co ...19989688218
a review of mechanisms underlying anticarcinogenicity by brassica vegetables.the mechanisms by which brassica vegetables might decrease the risk of cancer are reviewed in this paper. brassicas, including all types of cabbages, broccoli, cauliflower and brussels sprouts, may be protective against cancer due to their relatively high glucosinolate content. glucosinolates are usually broken down through hydrolysis catalyzed by myrosinase, an enzyme that is released from damaged plant cells. some of the hydrolysis products, viz. indoles and isothiocyanates, are able to influe ...19979055870
experimental study of oxidative dna damage.animal experiments allow the study of oxidative dna damage in target organs and the elucidation of dose-response relationships of carcinogenic and other harmful chemicals and conditions as well as the study of interactions of several factors. so far the effects of more than 50 different chemical compounds have been studied in animal experiments mainly in rats and mice, and generally with measurement of 8-oxodg with hplc-ec. a large number of well-known carcinogens induce 8-oxodg formation in liv ...199810098457
reduction of oxidative dna-damage in humans by brussels sprouts.the effect of consumption of brussels sprouts on levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodg) in human urine was investigated in 10 healthy, male, non-smoking volunteers. following a 3 week run-in period, five volunteers continued on a diet free of cruciferous vegetables for a subsequent 3 week intervention period (control group), while the other five (sprouts group) consumed 300 g of cooked brussels sprouts per day, at the expense of 300 g of a glucosinolate-free vegetable. levels of ...19957728983
behavior of listeria monocytogenes and aeromonas spp. on fresh-cut produce packaged under equilibrium-modified atmosphere.storage experiments were conducted to follow the behavior of pathogens on fresh-cut vegetables (trimmed brussels sprouts, grated carrots, shredded iceberg lettuce, and shredded chicory endives) packaged under an equilibrium-modified atmosphere (ema) (2 to 3% o2, 2 to 3% co2, and 94 to 96% n2) and stored at 7 degrees c. as a comparison, fresh-cut vegetables were also packaged in a perforated high-barrier film (air conditions) and stored at 7 degrees c. in a first step, the shelf life of the veget ...199910528715
brassica vegetables and cancer prevention. epidemiology and mechanisms.this paper first gives an overview of the epidemiological data concerning the cancer-preventive effect of brassica vegetables, including cabbages, kale, broccoli, brussels sprouts, and cauliflower. a protective effect of brassicas against cancer may be plausible due to their relatively high content of glucosinolates. certain hydrolysis products of glucosinolates have shown anticarcinogenic properties. the results of six cohort studies and 74 case-control studies on the association between brassi ...199910736624
susceptibility of four different vegetable brassicas to cabbage whitefly (aleyrodes proletella l., aleyrodidae) attack.cabbage whitefly (aleyrodes proletella l.) is one of the most noticeable pests on cultivated brassica species in the continental part of slovenia. in year 2000 a susceptibility of four different vegetables of brassica genus to cabbage whitefly attack had been examined. the highest number of imagoes and larvae was found on kale, significantly less on savoy cabbage and brussels sprouts and only individual specimen on cabbage. due to relatively small parcels, the most appropriate way of determining ...200212696419
inhibition of oxidative dna damage in vitro by extracts of brussels sprouts.cruciferous vegetables have cancer preventive effects which may be due to reduction of oxidative dna damage. we investigated the effect of an aqueous extract of cooked brussels sprouts on formation of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodg) in calf thymus dna in vitro. damage was induced by a fenton reaction, uvc (254 nm), uva (365 nm), sunlamp light, and methylene blue with visible light. the extract inhibited 8-oxodg formation in all systems except visible light with methylene blue. the ic50 ...200010885626
effects of brassica vegetable juice on the induction of apoptosis and aberrant crypt foci in rat colonic mucosal crypts in vivo.glucosinolates are sulfur-containing glycosides found in the brassica vegetables. their breakdown products include isothiocyanates, which are produced following exposure to the endogenous plant enzyme myrosinase. isothiocyanates are pungent, biologically active compounds that suppress carcinogenesis in vivo, and induce apoptosis in vitro. we have shown previously that oral administration of the isolated glucosinolate sinigrin induces apoptosis, and suppresses aberrant crypt foci in the colonic m ...200312663509
activation and potentiation of interferon-gamma signaling by 3,3'-diindolylmethane in mcf-7 breast cancer cells.3,3'-diindolylmethane (dim), a natural autolytic product in plants of the brassica genus, including broccoli, cauliflower, and brussels sprouts, exhibits promising cancer protective activities, especially against mammary neoplasia in animal models. we observed previously that dim induced a g(1) cell-cycle arrest and strong induction of cell-cycle inhibitor p21 expression and promoter activity in both estrogen-responsive and -independent breast cancer cell lines. we showed recently that dim up-re ...200616267208
protective effects of fruits and vegetables against in vivo clastogenicity of cyclosphosphamide or benzo[a]pyrene in mice.seven fruits and 10 vegetables commonly consumed in germany were investigated for their anticlastogenic potencies against cyclophosphamide (cp) and benzo[a]pyrene (bap) in the in vivo mouse bone marrow micronucleus assay. we detected protective effects in 76.5% and 70.6% of the samples, respectively, and more or less distinct quantitative differences between the various plant materials and the two clastogens investigated. with respect to cp, moderate activities were exerted by sweet cherries, st ...19989734714
consumption of brussels sprouts results in elevated alpha-class glutathione s-transferase levels in human blood plasma.the effect of consumption of glucosinolate-containing brussels sprouts on alpha-class glutathione s-transferase levels in human blood plasma was investigated in 10 healthy, male, non-smoking volunteers. following a 3-week run-in period, five volunteers continued on a glucosinolate-free diet during a subsequent 3-week intervention period (control group), while the other five (sprouts group) consumed 300 g of cooked brussels sprouts per day, at the expense of 300 g of a glucosinolate-free vegetabl ...19948200071
the effects of repeated applications of the molluscicide metaldehyde and the biocontrol nematode phasmarhabditis hermaphrodita on molluscs, earthworms, nematodes, acarids and collembolans: a two-year study in north-west spain.over two years, six consecutive field experiments were done in which the chemical molluscicide metaldehyde and the nematode biocontrol agent phasmarhabditis hermaphrodita (schneider) were applied at the standard field rates to replicated mini-plots successively planted with lettuce, brussels sprouts, leaf beet and cabbage, to compare the effectiveness of different treatments in reducing slug damage to the crops. soil samples from each plot were taken prior to the start of the experiments, and th ...200314620048
post-initiation treatment of indole-3-carbinol did not suppress n-methyl-n-nitrosourea induced mammary carcinogenesis in rats.the consumption of cruciferous vegetables (the family of cruciferae) such as cabbage, broccoli and brussels sprouts has been shown to have cancer chemopreventive effects in humans and experimental animals. indole-3-carbinol (i3c), one component of cruciferous vegetables, has been shown to exert cancer chemopreventive influence in liver, colon, and mammary tissue when given before or concurrent with exposure to a carcinogen. however in some reports, there has been evidence that consumption of i3c ...200111431103
in vitro and in vivo inhibition of human flavin-containing monooxygenase form 3 (fmo3) in the presence of dietary indoles.the effect of consumption of glucosinolate-containing brussels sprouts on flavin-containing monooxygenase functional activity in humans was investigated in 10 healthy, male, non-smoking volunteers. after a 3-week run-in period, 5 volunteers continued on a glucosinolate-free diet for 3 weeks (control group), and 5 others consumed 300 g of cooked brussels sprouts per day (sprouts group). human flavin-containing monooxygenase activity was measured by determining the levels of urinary trimethylamine ...199910509757
effect of common brassica vegetables (brussels sprouts and red cabbage) on the development of preneoplastic lesions induced by 2-amino-3-methylimidazo[4,5-f]quinoline (iq) in liver and colon of fischer 344 rats.aim of the present study was the investigation of effects of juices from commonly consumed brassica vegetables (two cultivars of brussels sprouts and two cultivars of red cabbage) on formation and development of preneoplastic lesions in colons (aberrant crypt foci, acf) and livers (glutathione-s-transferase placental form, gst-p+) in male f344 rats. the foci were induced by 2-amino-3-methylimidazo[4,5-f]quinoline (iq), a widespread carcinogenic heterocyclic aromatic amine which is found in fried ...200415036015
a brassica oleracea gene expressed in a variety-specific manner may encode a novel plant transmembrane receptor.the species brassica oleracea includes several agricultural varieties characterized by the proliferation of different types of meristems. using a combination of subtractive hybridization and pcr (polymerase chain reaction) techniques we have identified several genes which are expressed in the reproductive meristems of the cauliflower curd (b. oleracea var. botrytis) but not in the vegetative meristems of brussels sprouts (b. oleracea var. gemmifera) axillary buds. one of the cloned genes, termed ...200111333311
allylnitrile: generation from cruciferous vegetables and behavioral effects on mice of repeated exposure.the objective of the present study was to examine the possible generation of allylnitrile from commonly consumed cruciferous vegetables, and to determine the long-term behavioral effects of its oral administration at levels comparable to or greater than human dietary exposures. on the basis of gaschromatographic and mass spectrometric analyses, allylnitrile generation was observed in eight out of twelve vegetables, broccoli, broccoli (young stems and leaves), brussels sprouts, cabbage, cauliflow ...200414871587
pre-conceptional vitamin/folic acid supplementation 2007: the use of folic acid in combination with a multivitamin supplement for the prevention of neural tube defects and other congenital anomalies.to provide information regarding the use of folic acid in combination with a multivitamin supplement for the prevention of neural tube defects and other congenital anomalies, so that physicians, midwives, nurses, and other health care workers can assist in the education of women in the pre-conception phase of their health care. option: supplementation with folic acid and vitamins is problematic, since 50% of pregnancies are unplanned, and women's health status may not be optimal when they concei ...200718053387
protective effect of dietary brussels sprouts against mammary carcinogenesis in sprague-dawley rats.the effect of dietary brussels sprouts (brassica oleracea, l.) on mammary carcinogenesis induced by 7,12-dimethylbenz[a]anthracene (dmba) was studied in sprague-dawley female rats. rats fed a 20% brussels sprouts diet only during the initiation period of carcinogenesis had a palpable mammary tumor incidence of 13%, while those fed a casein-cornstarch semi-purified diet during this initiation period had a tumor incidence of 77% after 15 weeks post dmba dose. when the rats were switched from the s ...19883129179
protective effects of brussels sprouts, oligosaccharides and fermented milk towards 2-amino-3-methylimidazo[4,5-f]quinoline (iq)-induced genotoxicity in the human flora associated f344 rat: role of xenobiotic metabolising enzymes and intestinal microflora.we investigated the chemoprotective effects of four common constituents of the human diet, i.e. a fermented milk, inulin, oligofructose and brussels sprouts, towards 2-amino-3-methylimidazo[4,5-f]quinoline (iq)-induced genotoxicity in male fischer 344 rats harbouring a human intestinal microflora. we found that the four dietary components significantly reduced iq-induced dna damage in hepatocytes (reduction ranged from 74% with inulin to 39% with brussels sprouts) and colonocytes (reduction rang ...200415036016
cytostatic effects of 3,3'-diindolylmethane in human endometrial cancer cells result from an estrogen receptor-mediated increase in transforming growth factor-alpha expression.3,3'-diindolylmethane (dim), a major in vivo product of indole-3-carbinol (i3c), is a promising anticancer agent derived from vegetables of the brassica genus including broccoli, brussels sprouts and cabbage. we report here that dim has a potent cytostatic effect in cultured human ishikawa endometrial cancer cells. a combination of northern blot and quantitative pcr analyses revealed that dim induced the level of tgf-alpha transcripts by approximately 4-fold within 24 h of indole treatment. dim ...200111698343
protective effects of brussels sprouts towards b[a]p-induced dna damage: a model study with the single-cell gel electrophoresis (scge)/hep g2 assay.the aim of this study was to investigate the chemoprotective effects of brussels sprouts juice towards benzo[a]pyrene (b(a)p)-induced dna damage in the single-cell gel electrophoresis (scge)/hep g2 test system. this assay combines the advantages of the scge assay with that of the use of human-derived cells possessing inducible phase i and phase ii enzymes. co-treatment of hep g2 cells with small amounts of brussels sprouts juice (0.25-2.0 microl/ml) and b(a)p reduced the genotoxic effect of the ...200212067567
effect of cruciferous vegetable consumption on heterocyclic aromatic amine metabolism in man.the consumption of cooked meat appears to predispose individuals to colonic cancer and heterocyclic aromatic amines (ha), formed during the cooking of meat, have been suggested as aetiological agents. consumption of cruciferous vegetables is thought to protect against cancer. to study the effect of cruciferous vegetables on heterocyclic aromatic amine metabolism in man, a three-period, dietary intervention study has been carried out with 20 non-smoking caucasian male subjects consuming cooked me ...200111532863
isothiocyanates as cancer chemopreventive agents: their biological activities and metabolism in rodents and humans.isothiocyanates (itcs) are a group of naturally occurring compounds that occur as thioglucoside conjugates, termed glucosinolates, in plants and cruciferous vegetables such as watercress, brussels sprouts, broccoli, cabbage, kai choi, kale, horseradish, radish and turnip. itcs inhibit the development of tumors in many of the experimental models investigated, and are being investigated as possible chemopreventive agents for specific human cancers. the goal of this review is to provide a mechanist ...200212083319
chemoprevention of colon cancer by korean food plant components.inducible cyclooxygenase (cox-2) and inducible nitric oxide synthase (inos/nos-2) play pivotal roles as mediators of inflammation involved in early steps of carcinogenesis in certain organs. therefore, chemoprevention is theoretically possible through inhibition of cox-2 and/or inos. in the present study, we examined the chemopreventive effects of indole-3-carbinol (i3c), a constituent of cruciferous vegetables (the family of cruciferae) such as cabbages, cauliflowers and broccoli on the multipl ...200312628507
effect of mixed and single crops on disease suppressiveness of soils.abstract the effect of mixed cropping on disease suppressiveness of soils was tested for two cropping systems, brussels sprouts-barley and triticale-white clover. disease suppressiveness of field soils was evaluated in bioassays for the soilborne pathogens rhizoctonia solani, fusarium oxysporum f. sp. lini, and gaeumannomyces graminis var. tritici. for both cropping systems, mixed cropping did not enhance disease suppressiveness of the soils. in some cases, soil cropped to barley alone was signi ...200518943364
methods for the detection of antioxidants which prevent age related diseases: a critical review with particular emphasis on human intervention studies.it is well documented that reactive oxygen species (ros) are involved in the aetiology of age related diseases. over the last decades, strong efforts have been made to identify antioxidants in human foods and numerous promising compounds have been detected which are used for the production of supplements and functional foods. the present paper describes the advantages and limitations of methods which are currently used for the identification of antioxidants. numerous in vitro methods are availab ...200516077190
evaluation of urinary n-acetyl cysteinyl allyl isothiocyanate as a biomarker for intake and bioactivity of brussels sprouts.isothiocyanates (itc), glucosinolate hydrolysis products from brussels sprouts (bs) and other cruciferous vegetables, are considered to protect the body from cancer by induction of detoxification enzymes such as quinone reductase (qr). urinary n-acetyl-cysteine (nac) conjugates of itc have been proposed as biomarkers of crucifer intake. here we asked if dietary intake and induction of detoxification enzymes are dose-related to urinary nac conjugate appearance. male f344 rats (4/group) received a ...200314563407
antitumour promoting activity of indole-3-carbinol in mouse skin carcinogenesis.there has been growing interest in recent years in the potential of brassica vegetables (cabbage, cauliflower, brussels sprouts, etc.) as vectors for the introduction of anticarcinogenic compounds in the diet. indole-3-carbinol, a major indole metabolite present in the cruciferous vegetables, has been found to inhibit various rodent tumours when administered prior to or during carcinogen exposure. in this study, the antitumour promoting potential of indole-3-carbinol was studied in a two-stage m ...199810381134
3,3'-diindolylmethane is a novel topoisomerase iialpha catalytic inhibitor that induces s-phase retardation and mitotic delay in human hepatoma hepg2 cells.epidemiological evidence suggests that high consumption of brassica genus vegetables, such as broccoli, cabbage, and brussels sprouts, is very effective in reducing the risks of several types of cancers. 3,3'-diindolylmethane (dim), one of the most abundant and biologically active dietary compounds derived from brassica genus vegetables, displays remarkable antitumor activity against several experimental tumors. in the present study, we demonstrate for the first time that dim is a novel catalyti ...200616385077
signum, a new fungicide for control of leaf diseases in outdoor vegetables.during three years, the new fungicide signum, containing 6.7% pyraclostrobine + 26.7 % boscalid and developed by basf. has been evaluated in leek, carrots and cabbages in several outdoor field experiments under practical conditions and during one year in outdoor lettuce. in leek, phytophthora porri is one of the major leaf diseases causing lesions on differ ent places on the leaves, resulting in at least extra labour costs for trimming or even worse sometimes resulting in complete crop loss. so ...200516637178
absence of the glutathione s-transferase m1 gene increases cytochrome p4501a2 activity among frequent consumers of cruciferous vegetables in a caucasian population.the cancer protective effect of cruciferous vegetables has been attributed to induction of phase ii enzymes. but cruciferous vegetables also induce cytochrome p4501a2 (cyp1a2), which catalyzes the metabolic activation of various procarcinogens, including aromatic amines in tobacco. thus, frequent intake of cruciferous vegetables could also result in cancer-enhancing effects. gstm1 is involved in the detoxification of various carcinogens, but it also enhances the excretion of isothiocyanates and ...19989681534
molecular targets and anticancer potential of indole-3-carbinol and its derivatives.indole-3-carbinol (i3c) is produced by members of the family cruciferae, and particularly members of the genus brassica (e.g., cabbage, radishes, cauliflower, broccoli, brussels sprouts, and daikon). under acidic conditions, 13c is converted to a series of oligomeric products (among which 3,3'-diindolylmethane is a major component) thought to be responsible for its biological effects in vivo. in vitro, 13c has been shown to suppress the proliferation of various tumor cells including breast cance ...200516082211
indole-3-carbinol mediated cell cycle arrest of lncap human prostate cancer cells requires the induced production of activated p53 tumor suppressor protein.indole-3-carbinol (i3c), a dietary compound found naturally in cruciferous vegetables of the brassica genus such as broccoli and brussels sprouts, induces a g1 growth arrest of human reproductive cancer cells. we previously reported that in lncap prostate cancer cells, i3c down-regulated cyclin-dependent kinase (cdk) 2 activity. in our current study, western blotting and quantitative rt-pcr demonstrated that i3c treatment increased both the transcripts and protein levels of the cdk2 inhibitor p2 ...200616970927
effects of brussels sprouts extracts on hydrogen peroxide-induced dna strand breaks in human lymphocytes.aqueous brussels sprouts extracts inhibit oxidation of isolated dna in vitro, possibly through scavenging oxygen radicals. we have studied the effect of preincubating human lymphocytes with aqueous extracts of raw, cooked and autolysed brussels sprouts and the glucosinolate, sinigrin, on hydrogen peroxide-induced dna damage, strand breaks and base oxidation, in vitro by means of the comet assay. dna repair enzymes endonuclease iii (endoiii) and formamidopyrimidine-dna glycosylase (fpg) were used ...200111696392
the synergistic upregulation of phase ii detoxification enzymes by glucosinolate breakdown products in cruciferous vegetables.cruciferous vegetables contain secondary metabolites termed glucosinolates that break down to products that upregulate hepatic detoxification enzymes. we have previously shown that a mixture of four major glucosinolate breakdown products from brussels sprouts interact to produce synergistic induction of phase ii detoxification enzymes. here we tested the hypothesis that this synergism is at the level of transcription and is due to the interaction between the oral bifunctional inducer, indole-3-c ...200111446830
dietary antioxidants and cardiovascular disease.oxidative damage is involved in cardiovascular diseases. intervention with alpha-tocopherol, ascorbic acid and beta-carotene does not appear to reduce pathogenesis. the purpose of this review is to describe alternative antioxidant mechanisms that may be involved.200515650563
involvement of c-jun n-terminal kinase in g2/m arrest and caspase-mediated apoptosis induced by sulforaphane in du145 prostate cancer cells.sulforaphane (sfn) is a major isothiocyanate compound in cruciferous vegetables such as broccoli, cauliflower, and brussels sprouts. preclinical animal models have recently shown that sfn and other isothiocyanates may be useful for prostrate cancer (pca) chemoprevention. in this study we used a du145 human pca cell culture model to investigate the role of protein kinase signaling pathway(s) in sfn-induced cell cycle arrest and apoptosis and whether another chemopreventive agent selenium enhances ...200516201852
effects of brussels sprout juice on the cell cycle and adhesion of human colorectal carcinoma cells (ht29) in vitro.consumption of brassica vegetables is associated with a reduced risk of cancer of the alimentary tract in animal models and human populations. we used raw juice extracted from brussels sprouts rich in the glucosinolate sinigrin to explore the effect of naturally occurring glucosinolate breakdown products on cell cycle progression and apoptosis in human colorectal carcinoma cells (ht29). juice was prepared from sprout tissue immediately before use, and the glucosinolate breakdown products were de ...200515884814
chemoprevention of 2-amino-3-methylimidazo[4,5-f]quinoline (iq)-induced colonic and hepatic preneoplastic lesions in the f344 rat by cruciferous vegetables administered simultaneously with the carcinogen.the aim of this study was to investigate the chemopreventive effects of widely consumed cruciferous vegetables, namely brussels sprouts and red cabbage towards 2-amino-3-methylimidazo[4,5-f]quinoline (iq)-induced preneoplastic lesions [liver glutathione-s-transferase placental positive (gst-p(+)) foci and colonic aberrant crypt foci (acf)]. male f344 rats were treated with iq (100 mg/kg bw/g) on 10 alternating days and received drinking water supplemented with brussels sprouts and red cabbage ju ...200312584175
the dietary phytochemical indole-3-carbinol is a natural elastase enzymatic inhibitor that disrupts cyclin e protein processing.indole-3-carbinol (i3c), a naturally occurring component of brassica vegetables, such as broccoli, cabbage, and brussels sprouts, induces a g(1) cell-cycle arrest of human breast cancer cells, although the direct cellular targets that mediate this process are unknown. treatment of highly invasive mda-mb-231 breast cancer cells with i3c shifted the stable accumulation of cyclin e protein from the hyperactive lower-molecular-mass 35-kda form that is associated with cancer cell proliferation and po ...200819064917
induction of rat hepatic and intestinal udp-glucuronosyltransferases by naturally occurring dietary anticarcinogens.gastrointestinal tumours are among the most common malignancies in western society, the majority of which are associated with dietary and lifestyle factors. many dietary or lifestyle factors have been identified which may have toxic or carcinogenic properties. however, several dietary compounds also able to reduce gastrointestinal cancer rates in both humans and animals have been characterized. though the exact mechanism leading to the anticarcinogenic action of these compounds is not fully know ...200312869420
daily polyphenol intake in france from fruit and vegetables.the objective of this study was to create a french database on the polyphenol content of fruit and vegetables as uncooked fruits and vegetables and then to evaluate polyphenol intake through fruit and vegetable consumption in france. to achieve this, we used the folin-ciocalteu method adapted to fruit and vegetable polyphenol quantitation (1). vegetables with the highest polyphenol concentration were artichokes, parsley, and brussels sprouts [>250 mg of gallic acid equivalent (gae)/100 g fresh e ...200616920856
preclinical and clinical evaluation of sulforaphane for chemoprevention in the breast.consumers of higher levels of brassica vegetables, particularly those of the genus brassica (broccoli, brussels sprouts and cabbage), reduce their susceptibility to cancer at a variety of organ sites. brassica vegetables contain high concentrations of glucosinolates that can be hydrolyzed by the plant enzyme, myrosinase, or intestinal microflora to isothiocyanates, potent inducers of cytoprotective enzymes and inhibitors of carcinogenesis. oral administration of either the isothiocyanate, sulfor ...200717347138
indole-3-carbinol inhibits mda-mb-231 breast cancer cell motility and induces stress fibers and focal adhesion formation by activation of rho kinase activity.indole-3-carbinol (i3c), a phytochemical derived from cruciferous vegetables such as broccoli and brussels sprouts, has potent antiproliferative effects in human breast cancer cells and has been shown to decrease metastatic spread of tumors in experimental animals. using chemotaxis and fluorescent-bead cell motility assays, we demonstrated that i3c significantly decreased the in vitro migration of mda-mb-231 cells, a highly invasive breast cancer cell line. immunofluorescence staining of the act ...200919173291
effects of cruciferous vegetables and their constituents on drug metabolizing enzymes involved in the bioactivation of dna-reactive dietary carcinogens.epidemiological studies give evidence that cruciferous vegetables (cf) protect humans against cancer, and also results from animal experiments show that they reduce chemically induced tumor formation. these properties have been attributed to alterations in the metabolism of carcinogens by breakdown products of glucosinolates, which are constituents of cf. the present article gives an overview on the present state of knowledge on the impact of cf and their constituents on enzymes that are involve ...200111506821
food preferences and meal satisfaction of meals on wheels recipients.to investigate edmonton meals on wheels (mow) recipients' food preferences and meal satisfaction.200718073004
anti-proliferative activity and chemoprotective effects towards dna oxidative damage of fresh and cooked brassicaceae.epidemiological evidence shows that regular consumption of brassicaceae is associated with a reduced risk of cancer and heart disease. cruciferous species are usually processed before eating and the real impact of cooking practices on their bioactive properties is not fully understood. we have evaluated the effect of common cooking practices (boiling, microwaving, and steaming) on the biological activities of broccoli, cauliflower and brussels sprouts. anti-proliferative and chemoprotective effe ...201122088277
significance of terpenoids in induced indirect plant defence against herbivorous arthropods.many plants respond to herbivory by arthropods with an induced emission of volatiles such as green leaf volatiles and terpenoids. these herbivore-induced plant volatiles (hipvs) can attract carnivores, for example, predators and parasitoids. we investigated the significance of terpenoids in attracting herbivores and carnivores in two tritrophic systems where we manipulated the terpenoid emission by treating the plants with fosmidomycin, which inhibits one of the terpenoid biosynthetic pathways a ...200818208515
consumption of brussels sprouts protects peripheral human lymphocytes against 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (phip) and oxidative dna-damage: results of a controlled human intervention trial.to find out if the cancer protective effects of brussels sprouts seen in epidemiological studies are due to protection against dna-damage, an intervention trial was conducted in which the impact of vegetable consumption on dna-stability was monitored in lymphocytes with the comet assay. after consumption of the sprouts (300 g/p/d, n = 8), a reduction of dna-migration (97%) induced by the heterocyclic aromatic amine 2-amino-1-methyl-6-phenyl-imidazo-[4,5-b]pyridine (phip) was observed whereas no ...200818293303
putative mechanisms of action for indole-3-carbinol in the prevention of colorectal cancer.multiple factors are known to contribute to the development of cancer and numerous agents have been shown to confer some protection. diets high in vegetables, especially cruciferous vegetables (broccoli, brussels sprouts, cabbage etc.) provide such protection.200818479219
aqueous extract from spanish black radish (raphanus sativus l. var. niger) induces detoxification enzymes in the hepg2 human hepatoma cell line.spanish black radish (raphanus sativus l. var. niger) is a member of the cruciferae family that also contains broccoli and brussels sprouts, well-known to contain health-promoting constituents. spanish black radishes (sbr) contain high concentrations of a glucosinolate unique to the radish family, glucoraphasatin, which represents >65% of the total glucosinolates present in sbr. the metabolites of glucosinolates, such as isothiocyanates, are implicated in health promotion, although it is unclear ...200717616135
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