[phylogenetic diversity of the culturable rare actinomycetes in marine sponge hymeniacidon perlevis by improved isolation media].based on the molecular diversity information, seven actinomycete-selective culture media and isolation conditions were modified to isolate and cultivate diverse rare actinomycetes from hymeniacidon perlevis.200919873748
unusual symbiotic cyanobacteria association in the genetically diverse intertidal marine sponge hymeniacidon perlevis (demospongiae, halichondrida).cyanobacteria represent one of the most common members of the sponge-associated bacterial community and are abundant symbionts of coral reef ecosystems. in this study we used transmission electron microscopy (tem) and molecular techniques (16s rrna gene marker) to characterize the spatial distribution of cyanobionts in the widely dispersed marine intertidal sponge hymeniacidon perlevis along the coast of portugal (atlantic ocean). we described new sponge associated cyanobacterial morphotypes (xe ...201223251637
deoxyuridines from the marine sponge associated actinomycete streptomyces new nucleoside derivative, named 3-acetyl-5-methyl-2'-deoxyuridine (1), along with two known compounds 3,5-dimethyl-2'-deoxyuridine (2) and 3-methyl-2'-deoxyuridine (3), were isolated from the cultures of streptomyces microflavus. this strain was an associated actinomycete isolated from the marine sponge hymeniacidon perlevis collected from the coast of dalian (china). their structures were elucidated by detailed nmr and ms spectroscopic analysis as well as comparison with literature data.201121673882
isolation, characterization and identification of a paracoccus sp. 2-haloacid-degrading bacterium from the marine sponge hymeniacidon perlevis.a 2-haloacid dehalogenase-producing bacterium, designated deh99, was isolated from the marine sponge hymeniacidon perlevis using a modified enrichment medium and a ph indicator method. deh99 could degrade only half of the racemic mixture 2-chloropropionic acid (2-cpa) in the medium. the dehalogenase of deh99 was further determined to be a (s)-2-haloacid dehalogenase, which can degrade 2-cpa, 2-bromopropionic acid (2-bpa), and iodoacetic acid. the gene encoding the (s)-2-haloacid dehalogenase was ...201121298680
purification and characterization of a dehalogenase from pseudomonas stutzeri deh130 isolated from the marine sponge hymeniacidon perlevis.2-haloacid dehalogenases are enzymes that are capable of degrading 2-haloacid compounds. these enzymes are produced by bacteria, but so far they have only been purified and characterized from terrestrial bacteria. the present study describes the purification and characterization of 2-haloacid dehalogenase from the marine bacterium pseudomonas stutzeri deh130. p. stutzeri deh130 contained two kinds of 2-haloacid dehalogenase (designated as dehalogenase i and dehalogenase ii) as detected in the cr ...201323543208
evidence of unique and generalist microbes in distantly related sympatric intertidal marine sponges (porifera: demospongiae).the diversity and specificity of microbial communities in marine environments is a key aspect of the ecology and evolution of both the eukaryotic hosts and their associated prokaryotes. marine sponges harbor phylogenetically diverse and complex microbial lineages. here, we investigated the sponge bacterial community and distribution patterns of microbes in three sympatric intertidal marine demosponges, hymeniacidon perlevis, ophlitaspongia papilla and polymastia penicillus, from the atlantic coa ...201324265835
lindane bioremediation capability of bacteria associated with the demosponge hymeniacidon perlevis.lindane is an organochlorine pesticide belonging to persistent organic pollutants (pops) that has been widely used to treat agricultural pests. it is of particular concern because of its toxicity, persistence and tendency to bioaccumulate in terrestrial and aquatic ecosystems. in this context, we assessed the role of bacteria associated with the sponge hymeniacidon perlevis in lindane degradation. seven bacteria isolates were characterized and identified. these isolates showed a remarkable capac ...201728383507
the co-occurrence of the demosponge hymeniacidon perlevis and the edible mussel mytilus galloprovincialis as a new tool for bacterial load mitigation in aquaculture.pollutants in marine coastal areas are mainly a consequence of anthropogenic inputs, and microorganisms often play a major role in determining the extent of this pollution. thus, practical and eco-friendly techniques are urgently required in order to control or minimise the pathogenic bacterial problem. the bacterial accumulation of mytilus galloprovincialis (lamarck 1919) in the presence or absence of another filter feeder, the demosponge hymeniacidon perlevis (montagu 1818) on sewage flowing i ...201626498810
bioremediation of bacteria pollution using the marine sponge hymeniacidon perlevis in the intensive mariculture water system of turbot scophthalmus maximus.sessile filter-feeding marine sponges (porifera) have been reported to possess high efficiency in removing bacteria pollution from natural or aquaculture seawater. however, no investigation has been carried out thus far in a true mariculture farm water system. therefore this study sought to investigate the ability of the marine sponge hymeniacidon perlevis to bioremediate the bacteria pollution in the intensive aquaculture water system of turbot scophthalmus maximus. sponge specimens were hung i ...201019725041
growth and survival of early juveniles of the marine sponge hymeniacidon perlevis (demospongiae) under controlled resolve "the supply problem" in sponge-derived drug development and other biotechnological applications, current research is exploring the possibility of obtaining an alternative sustainable supply of sponge biomass through intensive aquaculture of sponges utilizing artificial seed rearing. this study aimed to investigate the technology of early juvenile sponge cultivation under controlled conditions. the effects of food, temperature, water flow, and light on the growth and survival of early ...200919221840
bioaccumulation of metallic trace elements and organic pollutants in marine sponges from the south brittany coast, france.the purpose of this study was to compare the accumulation of metallic and organic pollutants in marine sponges with the oyster crassostrea gigas used as sentinel species. the concentrations of 12 metallic trace elements (mtes), 16 polycyclic aromatic hydrocarbons (pahs), 7 polychlorinated biphenyls (pcbs), and 3 organotin derivatives were measured in 7 marine sponges collected in the etel river (south brittany, france). results indicated al, co, cr, fe, pb, and ti particularly accumulated in mar ...201626634290
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