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high-throughput gender identification of three columbidae species using melting curve analysis.the objective was to perform high-throughput gender identification of three columbidae species (columba livia, columba pulchricollis, and streptopelia tranquebarica). although the chromo-helicase-dna binding protein (chd)-based griffiths p2/p8 primer set resolved the amplicon products of these species in 3% agarose gel electrophoresis, it was unsuitable for molecular gender identification using the melting curve analysis (mca) curve for high-throughput analysis. after sequencing the chd-z and ch ...201120833415
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