Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| rna interference knockdown of aminopeptidase n genes decrease the susceptibility of chilo suppressalis larvae to cry1ab/cry1ac and cry1ca-expressing transgenic rice. | transgenic rice expressing bacillus thuringiensis (bt) cry toxins are resistant to lepidopteran pests, such as chilo suppressalis, a major insect pest of rice in asia. understanding how these toxins interact with their hosts is crucial to understanding their insecticidal action. in this study, knockdown of two aminopeptidase n genes (apn1 and apn2) by rna interference resulted in decreased susceptibility of c. suppressalis larvae to the bt rice varieties tt51 (cry1ab and cry1ac fusion genes) and ... | 2017 | 28274717 |
| sublethal effects of chlorantraniliprole on juvenile hormone levels and mrna expression of jhamt and fpps genes in the rice stem borer, chilo suppressalis. | juvenile hormone (jh) regulates development and reproduction of insects. the sublethal effects of chlorantraniliprole on jh levels and mrna expression of jh acid methyltransferase gene (csjhamt) and farnesyl diphosphate synthase genes (csfpps1 and csfpps2 ) in chilo suppressalis (walker) were investigated. | 2017 | 28382786 |
| expression of lima bean terpene synthases in rice enhances recruitment of a beneficial enemy of a major rice pest. | volatile terpenoids play a key role in plant defence against herbivory by attracting parasitic wasps. we identified seven terpene synthase genes from lima bean, phaseolus lunatus l. following treatment with either the elicitor alamethicin or spider mites, tetranychus cinnabarinus. four of which (pltps2, pltps3, pltps4 and pltps5) were upregulated, and their derived proteins are phylogenetically clustered in the tps-g subfamily, with pltps3 positioned at the base of this cluster. recombinant pltp ... | 2017 | 28370092 |
| knockdown of the mapk p38 pathway increases the susceptibility of chilo suppressalis larvae to bacillus thuringiensis cry1ca toxin. | the bacterium bacillus thuringiensis (bt) produces a wide range of toxins that are effective against a number of insect pests. identifying the mechanisms responsible for resistance to bt toxin will improve both our ability to control important insect pests and our understanding of bacterial toxicology. in this study, we investigated the role of mapk pathways in resistance against cry1ca toxin in chilo suppressalis, an important lepidopteran pest of rice crops. we first cloned the full-length of ... | 2017 | 28262736 |
| rnai in the striped stem borer, chilo suppressalis, establishes a functional role for aminopeptidase n in cry1ab intoxication. | the striped stem borer, chilo suppressalis, is a major target pest of transgenic rice expressing the cry1ab protein from the bacterium bacillus thuringiensis (bt) in china. evolution of resistance in this pest is a major threat to the durability of bt rice. since bt exerts its activity through binding to specific receptors in the midgut of target insects, identification of functional cry1ab receptors in the midgut of c. suppressalis larvae is crucial to evaluate potential resistance mechanisms a ... | 2017 | 27823898 |
| difference in diel mating time contributes to assortative mating between host plant-associated populations of chilo suppressalis. | behavioral isolation in animals can be mediated by inherent mating preferences and assortative traits, such as divergence in the diel timing of mating activity. although divergence in the diel mating time could, in principle, promote the reproductive isolation of sympatric, conspecific populations, there is currently no unequivocal evidence of this. we conducted different mate-choice experiments to investigate the contribution of differences in diel mating activity to the reproductive isolation ... | 2017 | 28338099 |
| pharmacological characterization of dopamine receptors in the rice striped stem borer, chilo suppressalis. | dopamine is an important neurotransmitter and neuromodulator in both vertebrates and invertebrates and is the most abundant monoamine present in the central nervous system of insects. a complement of functionally distinct dopamine receptors mediate the signal transduction of dopamine by modifying intracellular ca(2+) and camp levels. in the present study, we pharmacologically characterized three types of dopamine receptors, csdop1, csdop2 and csdop3, from the rice striped stem borer, chilo suppr ... | 2017 | 28302436 |
| selection and evaluation of reference genes for expression analysis using qrt-pcr in chilo suppressalis (lepidoptera: pyralidae). | quantitative real-time polymerase chain reaction (qrt-pcr) is a valuable tool for estimating gene expression; however, the validity is largely dependent on the selection of stable reference genes. the suitability of various reference genes for qrt-pcr analysis was evaluated in, chilo suppressalis (walker). the δct method, genorm, normfinder, and bestkeeper were used to evaluate the suitability of nine candidate reference genes for normalizing gene expression in larval tissues and organs and duri ... | 2017 | 28115499 |
| ryanodine receptor genes of the rice stem borer, chilo suppressalis: molecular cloning, alternative splicing and expression profiling. | the ryanodine receptor (ryr) of the calcium release channel is the main target of anthranilic and phthalic diamide insecticides which have high selective insecticidal activity relative to mammalian toxicity. in this study, the full-length cdna of chilo suppressalis ryr (csryr) was isolated and characterized. the csryr mrna has an open reading frame (orf) of 15,387bp nucleotides, which encodes 5128 amino acids with genbank id: kr088972. comparison of protein sequences showed that csryr shared hig ... | 2017 | 28043334 |
| large-scale identification of differentially expressed genes during pupa development reveals solute carrier gene is essential for pupal pigmentation in chilo suppressalis. | insects undergo metamorphosis, involving an abrupt change in body structure through cell growth and differentiation. rice stem stripped borer (ssb), chilo suppressalis, is one of the most destructive rice pests. however, little is known about the regulation mechanism of metamorphosis development in this notorious insect pest. here, we studied the expression of 22,197 ssb genes at seven time points during pupa development with a customized microarray, identifying 622 differentially expressed gene ... | 2017 | 28041944 |
| thermal effects on development and adult longevity of endoparasitoid chelonus murakatae munakata (hymenoptera: braconidae). | the effects of temperature on the development duration and longevity of adult of chelonus murakatae were studied under five constant temperatures including 22.5, 25, 27.5, 30 and 32.5 ± 0.5 °c under laboratory conditions. it was observed that the development time was inversely proportional to the temperature within the range of 22.5 to 32.5 °c. the results indicated that the optimum temperature for development ranged from 25 to 30 °c. thermal threshold was estimated by a linear model which was r ... | 2017 | 27988903 |
| combined transcriptome and metabolome analyses to understand the dynamic responses of rice plants to attack by the rice stem borer chilo suppressalis (lepidoptera: crambidae). | rice (oryza sativa l.), which is a staple food for more than half of the world's population, is frequently attacked by herbivorous insects, including the rice stem borer, chilo suppressalis. c. suppressalis substantially reduces rice yields in temperate regions of asia, but little is known about how rice plants defend themselves against this herbivore at molecular and biochemical level. | 2016 | 27923345 |
| the overexpression of insect endogenous small rnas in transgenic rice inhibits growth and delays pupation of striped stem borer (chilo suppressalis). | the striped stem borer (ssb), chilo suppressalis walker, is a major rice insect pest worldwide. rna interference (rnai) has become a promising strategy for developing insect-resistant crops. in a previous study, five double-stranded rnas (dsrnas) targeting important ssb housekeeping genes were overexpressed in rice, but none of the acquired dsrna-transgenic rice plants showed significant effects on ssb. | 2016 | 27862861 |
| sublethal effects of chlorantraniliprole on development, reproduction and vitellogenin gene (csvg) expression in the rice stem borer, chilo suppressalis. | the rice stem borer, chilo suppressalis (walker), is one of the most damaging rice pests in the world. the sublethal effects of chlorantraniliprole on development,reproduction and mrna expression levels of vitellogenin gene (csvg) in c. suppressalis were investigated. | 2016 | 26939546 |
| variability in development of the striped rice borer, chilo suppressalis (lepidoptera: pyralidae), due to instar number and last instar duration. | the striped stem borer, chilo suppressalis (walker), is an important insect pest of rice which shows substantial variation in developmental duration among individuals. this variation is currently poorly characterized but it is important from a control perspective because pesticides can only target early sensitive instars. it is unclear whether there are key stages that determine the length of developmental duration of individuals and/or whether variation in instar number contributes to this vari ... | 2016 | 27731388 |
| human health risk assessment of organophosphorus pesticide in rice crop from selected districts of anzali international wetland basin, iran. | low fertility in rice caused by chilo suppressalis has led to the use of diazinon to control this pest. residue of pesticide could penetrate products and also food which can affect public health. the aim of this research was to determine health risk assessment of organophosphorus (op) pesticide in rice, a strategic crop in iran. ninety rice samples were collected from 30 points during harvesting seasons from rasht area, guilan province, iran from which 30 samples were prepared. the concentration ... | 2016 | 27417637 |
| antixenosis and antibiosis resistance in rice cultivars against chilo suppressalis (walker) (lepidoptera: crambidae). | the striped stem borer, chilo suppressalis (walker) (lepidoptera: crambidae), is an important pest afflicting rice in most rice-growing countries in the world. deliniating the categories of resistance in rice genotypes under field conditions could be helpful in managment of this pest. two categories of resistance, antixenosis and antibiosis, were examined in ten popular and diverse rice genotypes of different origin that had been selected for their resistance to the striped stem borer in a previ ... | 2016 | 28039677 |
| do differences in life-history traits and the timing of peak mating activity between host-associated populations of chilo suppressalis have a genetic basis? | the development of host races, genetically distinct populations of the same species with different hosts, is considered to be the initial stage of ecological speciation. ecological and biological differences consistent with host race formation have been reported between water-oat and rice-associated populations of chilo suppressalis. in order to confirm whether these differences have a genetic basis, we conducted experiments to determine the extent to which various life-history traits and the ti ... | 2016 | 27386090 |
| distinct contributions of a314s and novel r667q substitutions of acetylcholinesterase 1 to carbofuran resistance of chilo suppressalis walker. | in the striped stem borer, chilo suppressalis, a314s, r667q and h669p substitutions in acetylcholinesterase 1 (csache1) have been associated with >1000-fold resistance against carbofuran. in this study, eight variants of csache1 carrying different combinations of these substitutions were cloned and expressed using the bac-to-bac expression system to verify their contributions. | 2016 | 26446949 |
| oswrky53, a versatile switch in regulating herbivore-induced defense responses in rice. | wrky proteins, which belong to a large family of plant-specific transcription factors, play important roles in plant defenses against pathogens and herbivores by regulating defense-related signaling pathways. recently, a rice wrky transcription factor oswrky53 has been reported to function as a negative feedback modulator of osmpk3/osmpk6 and thereby to control the size of the investment a rice plant makes to defend against a chewing herbivore, the striped stem borer chilo suppressalis. we inves ... | 2016 | 27031005 |
| molecular characterization and expression profiles of nicotinic acetylcholine receptors in the rice striped stem borer, chilo suppressalis (lepidoptera: crambidae). | nicotinic acetylcholine receptors (nachrs) are members of the cys-loop ligand-gated ion channel (cyslgic) superfamily, mediating fast synaptic cholinergic transmission in the central nervous system in insects. insect nachrs are the molecular targets of economically important insecticides, such as neonicotinoids and spinosad. identification and characterization of the nachr gene family in the rice striped stem borer, chilo suppressalis, could provide beneficial information about this important re ... | 2016 | 26847606 |
| identification of novel odorant binding protein genes and functional characterization of obp8 in chilo suppressalis (walker). | at the peripheral level of the insect olfaction, odorant binding proteins (obps) are thought to bind and transport exogenous hydrophobic volatiles to the odorant receptors (ors) located on the dendrite membrane of the olfactory neurons. in this study, cdna sequences of 29 obp genes from chilo suppressalis, a notorious rice pest, were identified, with 15 of them being newly reported. the tissue and temporal expression patterns of these csupobps were determined by rt-pcr, revealing that csupobp8, ... | 2016 | 27374155 |
| identification and expression profiles of neuropeptides and their g protein-coupled receptors in the rice stem borer chilo suppressalis. | in insects, neuropeptides play important roles in the regulation of multiple physiological processes by binding to their corresponding receptors, which are primarily g protein-coupled receptors (gpcrs). the genes encoding neuropeptides and their associated gpcrs in the rice stem borer chilo suppressalis were identified by a transcriptomic analysis and were used to identify potential targets for the disruption of physiological processes and the protection of crops. forty-three candidate genes wer ... | 2016 | 27353701 |
| life-history responses of the rice stem borer chilo suppressalis to temperature change: breaking the temperature-size rule. | temperature is a key environmental factor for ectotherms and affects a large number of life history traits. in the present study, development time from hatching to pupation and adult eclosion, pupal and adult weights of the rice stem borer, chilo suppressalis were examined at 22, 25, 28 and 31°c under l18:d 6. larval and pupal times were significantly decreased with increasing rearing temperature and growth rate was positively correlated with temperature. larval and pupal developmental times wer ... | 2016 | 27712652 |
| transition and transversion mutations are biased towards gc in transposons of chilo suppressalis (lepidoptera: pyralidae). | transposons are often regulated by their hosts, and as a result, there are transposons with several mutations within their host organisms. to gain insight into the patterns of the variations, nucleotide substitutions and indels of transposons were analysed in chilo suppressalis walker. the csuple1.1 is a member of the piggybac-like element (ple) family, which belongs to the dna transposons, and the csu-ty3 is a member of the ty3/gypsy family, which belongs to the rna transposons. copies of csupl ... | 2016 | 27669309 |
| monitoring and mechanisms of insecticide resistance in chilo suppressalis (lepidoptera: crambidae), with special reference to diamides. | the rice stem borer, chilo suppressalis walker (lepidoptera: crambidae), is one of the most economically important pests of rice in asia. chemical control remains the most efficient primary means for controlling this pest. | 2016 | 27624654 |
| genome-wide analysis of chitinase genes and their varied functions in larval moult, pupation and eclosion in the rice striped stem borer, chilo suppressalis. | some insect chitinases are required to degrade chitin and ensure successful metamorphosis. although chitinase genes have been well characterized in several model insects, no reports exist for the rice striped stem borer, chilo suppressalis, a highly destructive pest that causes huge yield losses in rice production. here, we conducted a genome-level analysis of chitinase genes in c. suppressalis. after amplification of full-length transcripts with rapid amplification of cdna ends, we identified 1 ... | 2016 | 27080989 |
| the effect of silencing 20e biosynthesis relative genes by feeding bacterially expressed dsrna on the larval development of chilo suppressalis. | rna interference (rnai) is a robust tool to study gene functions as well as potential for insect pest control. finding suitable target genes is the key step in the development of an efficient rnai-mediated pest control technique. based on the transcriptome of chilo suppressalis, 24 unigenes which putatively associated with insect hormone biosynthesis were identified. amongst these, four genes involved in ecdysteroidogenesis i.e., ptth, torso, spook and nm-g were evaluated as candidate targets fo ... | 2016 | 27352880 |
| parasitism and suitability of fertilized and nonfertilized eggs of the rice striped stem borer, chilo suppressalis (lepidoptera: crambidae), for trichogramma parasitoids. | parasitoids can utilize the nutrition of host eggs to culture their own offsprings. host qualities, including size, age, and species, influence parasitism preference and fitness of the parasitoid offsprings. however, an interesting question here is whether the parasitoids have the ability of recognizing fertilized and nonfertilized host eggs. in this study, we investigated the performance of three indigenous trichogramma species (trichogramma japonicum ashmead, trichogramma dendrolimi matsumura, ... | 2016 | 26802170 |
| effects of the endoparasitoid cotesia chilonis (hymenoptera: braconidae) parasitism, venom, and calyx fluid on cellular and humoral immunity of its host chilo suppressalis (lepidoptera: crambidae) larvae. | the larval endoparasitoid cotesia chilonis injects venom and bracoviruses into its host chilo suppressalis during oviposition. here we study the effects of the polydnavirus (pdv)-carrying endoparasitoid c. chilonis (hymenoptera: braconidae) parasitism, venom and calyx fluid on host cellular and humoral immunity, specifically hemocyte composition, cellular spreading, encapsulation and melanization. total hemocyte counts (thcs) were higher in parasitized larvae than in unparasitized larvae in the ... | 2016 | 26685058 |
| comparison of three transgenic bt rice lines for insecticidal protein expression and resistance against a target pest, chilo suppressalis (lepidoptera: crambidae). | two transgenic rice lines (t2a-1 and t1c-19b) expressing cry2a and cry1c genes, respectively, were developed in china, targeting lepidopteran pests including chilo suppressalis (walker) (lepidoptera: crambidae). the seasonal expression of cry proteins in different tissues of the rice lines and their resistance to c. suppressalis were assessed in comparison to a bt rice line expressing a cry1ab/ac fusion gene, huahui 1, which has been granted a biosafety certificate. in general, levels of cry pro ... | 2016 | 25284137 |
| new ideas about genetic differentiation of chilo suppressalis (lepidoptera: pyralidae) populations in china based on the mtdna cytochrome b gene. | the striped stem borer, chilo suppressalis (walker), is an important pest of rice in china and other parts of the world. to further explore the population genetic structure and genetic differentiation of c. suppressalis populations found on rice in china, we amplified 432 bp fragments of the cytochrome b (cyt b) gene for 44 c. suppressalis populations. nineteen variable sites in the mtdna gene were observed, and 16 haplotypes were identified. nucleotide diversity (π) and haplotype diversity (h) ... | 2016 | 25211083 |
| pyramiding and evaluation of both a foreign bacillus thuringiensis and a lysine-rich protein gene in the elite indica rice 9311. | gene pyramiding is an efficient approach for the genetic improvement of multiple agronomic traits simultaneously. in this study, we pyramided two foreign genes, cry1ac driven by the rice actin i promoter, and lysine-rich protein (lrp), driven by the endosperm-specific glutelin1 (gt1) promoter, into the elite indica cultivar 9311. these two genes were chosen in an attempt to enhance insect-resistance and lysine (lys) content. in the pyramided line, the foreign gene cry1ac was efficiently expresse ... | 2016 | 27795684 |
| development of bt rice and bt maize in china and their efficacy in target pest control. | rice and maize are important cereal crops that serve as staple foods, feed, and industrial material in china. multiple factors constrain the production of both crops, among which insect pests are an important one. lepidopteran pests cause enormous yield losses for the crops annually. in order to control these pests, china plays an active role in development and application of genetic engineering (ge) to crops, and dozens of ge rice and ge maize lines expressing insecticidal proteins from the soi ... | 2016 | 27763554 |
| comparison of susceptibility of chilo suppressalis and bombyx mori to five bacillus thuringiensis proteins. | transformation of rice with genes encoding insecticidal cry proteins from bacillus thuringiensis (bt) should confer high resistance to target lepidopteran pests, such as chilo suppressalis, and low toxicity to non-target organisms, such as silkworm bombyx mori. five purified cry proteins that have been used for plant transformation were tested using dietary exposure assays. the susceptibility of c. suppressalis larvae to the five insecticidal proteins in the decreasing order was: cry1ca>cry1ab>c ... | 2016 | 26994840 |
| development of marker-free insect-resistant indica rice by agrobacterium tumefaciens-mediated co-transformation. | agrobacterium-mediated co-transformation is an efficient strategy to generate marker-free transgenic plants. in this study, the vectors pmf-2a(∗) containing a synthetic cry2a(∗) gene driven by maize ubiquitin promoter and pcambia1301 harboring hygromycin phosphotransferase gene (hpt) were introduced into minghui86 (oryza sativa l. ssp. indica), an elite indica restorer line. two independent transformants containing both the cry2a(∗) gene and hpt gene were regenerated. several homozygous marker-f ... | 2016 | 27833629 |
| conserved chemosensory proteins in the proboscis and eyes of lepidoptera. | odorant-binding proteins (obps) and chemosensory proteins (csps) are endowed with several different functions besides being carriers for pheromones and odorants. based on a previous report of a csp acting as surfactant in the proboscis of the moth helicoverpa armigera, we revealed the presence of orthologue proteins in two other moths plutella xylostella and chilo suppressalis, as well as two butterflies papilio machaon and pieris rapae, using immunodetection and proteomic analysis. the unusual ... | 2016 | 27877091 |
| insectbase: a resource for insect genomes and transcriptomes. | the genomes and transcriptomes of hundreds of insects have been sequenced. however, insect community lacks an integrated, up-to-date collection of insect gene data. here, we introduce the first release of insectbase, available online at http://www.insect-genome.com. the database encompasses 138 insect genomes, 116 insect transcriptomes, 61 insect gene sets, 36 gene families of 60 insects, 7544 mirnas of 69 insects, 96,925 pirnas of drosophila melanogaster and chilo suppressalis, 2439 lncrna of n ... | 2015 | 26578584 |
| identification of putative carboxylesterase and glutathione s-transferase genes from the antennae of the chilo suppressalis (lepidoptera: pyralidae). | in insects, rapid degradation of odorants in antennae is extremely important for the sensitivity of olfactory receptor neurons. odorant degradation in insect antennae is mediated by multiple enzymes, especially the carboxylesterases (cxes) and glutathione s-transferases (gsts). the asiatic rice borer, chilo suppressalis, is an economically important lepidopteran pest which causes great economic damage to cultivated rice crops in many asian countries. in this study, we identified 19 putative cxe ... | 2015 | 26198868 |
| efficient generation of marker-free transgenic rice plants using an improved transposon-mediated transgene reintegration strategy. | marker-free transgenic plants can be developed through transposon-mediated transgene reintegration, which allows intact transgene insertion with defined boundaries and requires only a few primary transformants. in this study, we improved the selection strategy and validated that the maize (zea mays) activator/dissociation (ds) transposable element can be routinely used to generate marker-free transgenic plants. a ds-based gene of interest was linked to green fluorescent protein in transfer dna ( ... | 2015 | 25371551 |
| a 90-day subchronic feeding study of genetically modified rice expressing cry1ab protein in sprague-dawley rats. | bacillus thuringiensis (bt) transgenic rice line (mfb-mh86) expressing a synthetic cry1ab gene can be protected against feeding damage from lepidopteran insects, including sesamia inferens, chilo suppressalis, tryporyza incertulas and cnaphalocrocis medinalis. rice flour from mfb-mh86 and its near-isogenic control mh86 was separately formulated into rodent diets at concentrations of 17.5, 35 and 70 % (w/w) for a 90-day feeding test with rats, and all of the diets were nutritionally balanced. in ... | 2015 | 25367203 |
| chilo suppressalis and sesamia inferens display different susceptibility responses to cry1a insecticidal proteins. | chilo suppressalis and sesamia inferens are important lepidopteran rice pests that occur concurrently in rice-growing areas of china. the development of transgenic rice expressing cry1a insecticidal proteins has provided a useful strategy for controlling these pests. | 2015 | 25469810 |
| knockdown of cs-spook induces delayed larval molting in rice striped stem borer chilo suppressalis. | spook has essential roles in the biogenesis of the molting hormone 20-hydroxyecdysone (20-e). the function of spook in the rice striped stem borer (ssb) chilo suppressalis remains unclear, prompting our hypothesis that it exerts actions similar to those reported for other insect species. here we amplified the full-length transcript of spook (cs-spook) in ssb by 5' and 3' rapid amplification of cdna ends. cs-spook has conserved p450 motifs such as helix-c, helix-i, helix-k, and perf motif (pxxfxp ... | 2015 | 25354095 |
| transcriptome analysis of an endoparasitoid wasp cotesia chilonis (hymenoptera: braconidae) reveals genes involved in successful parasitism. | for successful parasitization, parasitiods usually depend on the chemosensory cues for the selection of hosts, as well as a variety of virulence factors introduced into their hosts to overcome host immunity and prevent rejection of progeny development. in bracovirus-carrying wasps, the symbiotic polydnaviruses act in manipulating development and immunity of hosts. the endoparasitoid cotesia chilonis carrying bracovirus as a key host immunosuppressive factor is a superior endoparasitoid of rice s ... | 2015 | 25336406 |
| characterization of competitive interactions in the coexistence of bt-transgenic and conventional rice. | transgene flow through pollen and seeds leads to transgenic volunteers and feral populations in the nature, and consumer choice and economic incentives determine whether transgenic crops will be cultivated in the field. transgenic and non-transgenic plants are likely to coexist in the field and natural habitats, but their competitive interactions are not well understood. | 2015 | 25928331 |
| characterization of a tyramine receptor type 2 from hemocytes of rice stem borer, chilo suppressalis. | calcium acts as a second messenger in many cell types, including insect hemocytes. intracellular calcium level has a definite role in innate and adaptive immune signaling. biogenic amines such as octopamine (oa), tyramine (ta), dopamine (da) and serotonin (5-ht) play various important physiological roles in insects by activating distinct g-protein-coupled receptors (gpcrs) that share a putative seven transmembrane domain structure. oa and 5-ht have been shown that can mediate insect hemocytic im ... | 2015 | 25772095 |
| large number of putative chemoreception and pheromone biosynthesis genes revealed by analyzing transcriptome from ovipositor-pheromone glands of chilo suppressalis. | the chemoreception role of moth ovipositor has long been suggested, but its molecular mechanism is mostly unknown. by transcriptomic analysis of the female ovipositor-pheromone glands (ov-pg) of chilo suppressalis, we obtained 31 putative chemoreception genes (9 obps, 10 csps, 2 ors, 1 snmp, 8 cxes and 1 aox), in addition to 32 genes related to sex pheromone biosynthesis (1 fas, 6 dess, 10 fars, 2 acos, 1 acc, 4 fatps, 3 acbps and 5 elos). tissue expression profiles further revealed that csupcsp ... | 2015 | 25601555 |
| loss of genetic variation in laboratory colonies of chilo suppressalis (lepidoptera: crambidae) revealed by mitochondrial and microsatellite dna markers. | the asiatic rice borer, chilo suppressalis (walker), is an important insect pest of rice in china. the genetic variation of a set of laboratory colonies of c. suppressalis was compared with their source populations in the wild (laboratory colonies bjck, bj1ab, and bj1ac versus wild population bjw; laboratory colonies fzck and fz1ca versus wild population fzw) and was analyzed using eight microsatellite markers and two partial mitochondrial dna (mtdna) regions (coi and coii). results from both an ... | 2015 | 26308808 |
| genome-wide analysis of esterase-like genes in the striped rice stem borer, chilo suppressalis. | the striped rice stem borer, chilo suppressalis, a destructive pest of rice, has developed high levels of resistance to certain insecticides. esterases are reported to be involved in insecticide resistance in several insects. therefore, this study systematically analyzed esterase-like genes in c. suppressalis. fifty-one esterase-like genes were identified in the draft genomic sequences of the species, and 20 cdna sequences were derived which encoded full- or nearly full-length proteins. the puta ... | 2015 | 26285093 |
| de novo assembly and characterization of central nervous system transcriptome reveals neurotransmitter signaling systems in the rice striped stem borer, chilo suppressalis. | neurotransmitter signaling systems play crucial roles in multiple physiological and behavioral processes in insects. genome wide analyses of de novo transcriptome sequencing and gene specific expression profiling provide rich resources for studying neurotransmitter signaling pathways. the rice striped stem borer, chilo suppressalis is a destructive rice pest in china and other asian countries. the characterization of genes involved in neurotransmitter biosynthesis and transport could identify po ... | 2015 | 26173787 |
| prioritizing plant defence over growth through wrky regulation facilitates infestation by non-target herbivores. | plants generally respond to herbivore attack by increasing resistance and decreasing growth. this prioritization is achieved through the regulation of phytohormonal signaling networks. however, it remains unknown how this prioritization affects resistance against non-target herbivores. in this study, we identify wrky70 as a specific herbivore-induced, mitogen-activated protein kinase-regulated rice transcription factor that physically interacts with w-box motives and prioritizes defence over gro ... | 2015 | 26083713 |
| pheromone binding proteins enhance the sensitivity of olfactory receptors to sex pheromones in chilo suppressalis. | sexual communication in moths offers a simplified scenario to model and investigate insect sensory perception. both pbps (pheromone-binding proteins) and prs (pheromone receptors) are involved in the detection of sex pheromones, but the interplay between them still remains largely unknown. in this study, we have measured the binding affinities of the four recombinant pbps of chilo suppressalis (csuppbps) to pheromone components and analogs and characterized the six prs using the xenopus oocytes ... | 2015 | 26310773 |
| immune and metabolic responses of chilo suppressalis walker (lepidoptera: crambidae) larvae to an insect growth regulator, hexaflumuron. | efficient control of chilo suppressalis walker is always controversial due to highly economic damage, resistance to insecticides and environmental pollutions. so, combination of safe pest controls e.g. biocontrol agents and insect growth regulators seems to be promising via integrated pest management program. bioassay of hexaflumuron on 4th larval instars revealed concentrations of 44.34, 179.74 and 474.94µg/ml as lc10-50 values. numbers of total hemocytes, plasmatocytes and granulocytes as well ... | 2015 | 26615153 |
| dopamine modulates hemocyte phagocytosis via a d1-like receptor in the rice stem borer, chilo suppressalis. | dopamine (da) is a signal moiety bridging the nervous and immune systems. da dysregulation is linked to serious human diseases, including addiction, schizophrenia, and parkinson's disease. however, da actions in the immune system remain incompletely understood. in this study, we found that da modulates insect hemocyte phagocytosis using hemocytes prepared from the rice stem borer (rsb), chilo suppressalis. we investigated whether insect hemocytes are capable of de novo da production. here we sho ... | 2015 | 26179416 |
| construction and analysis of antennal cdna library from rice striped stem borer, chilo suppressalis (walker) (lepidoptera: pyralidae), and expression profiles of putative odorant-binding protein and chemosensory protein genes. | in this study, we constructed a high-quality cdna library from the antennae of the chilo suppressalis (walker) (lepidoptera: pyralidae). a total of 1,235 colonies with inserts greater than 0.7 kb were sequenced and analyzed. homology searching coupled with bioinformatics analysis identified 15 and 7 cdna sequences, respectively, encoding putative odorant-binding proteins (obps) and chemosensory proteins (csps). a phylogenetic tree of csupcsps showed that each csupcsp has orthologs in manduca sex ... | 2015 | 25639603 |
| establishment and characterization of a new cell line of chilo suppressalis walker (lepidoptera: pyralididae). | a new cell line, designated as zjbiq-chsu-i, was initiated from the fat body of larval chilo suppressalis (walker) (lepidoptera: pyralididae) in tnm-fh insect medium containing 15% fetal bovine serum. the polygonal cells (65.6%) were predominant among various cell types, and the diameter range was from 12.63 to 22.50 μm. the cell line showed a typical lepidopteran chromosome pattern ranging from 108 to 136 chromosomes in the majority of the cells. the population doubling time (pdt) of the cell l ... | 2015 | 25381037 |
| cloning of two acetylcholinesterase genes and analysis of point mutations putatively associated with triazophos resistance in chilo auricilius (lepidoptera: pyralidae). | acetylcholinesterase (ache) is the target of organophosphate (op) and carbamate insecticides. mutations in the ache gene (ace) leading to decreased insecticide susceptibility is the main resistance mechanism in insects. in this study, two chilo auricilius acetylcholinesterase genes, designated as caace1 and caace2, were cloned using rt-pcr and race. caace1 cdna is 2534 bp, with orf of 2082 bp, and it encodes an acetylcholinesterase 1 (caache1) protein comprising a calculated 693 amino acid (aa) ... | 2015 | 26470257 |
| bt rice does not disrupt the host-searching behavior of the parasitoid cotesia chilonis. | we determined whether plant volatiles help explain why cotesia chilonis (a parasitoid of the target pest chilo suppressalis) is less abundant in bt than in non-bt rice fields. olfactometer studies revealed that c. chilonis females responded similarly to undamaged bt and non-bt rice plants. parasitoids preferred rice plants damaged by 3(rd)-instar larvae of c. suppressalis, but did not differentiate between caterpillar-infested bt and non-bt plants. according to gc-ms analyses of rice plant volat ... | 2015 | 26470012 |
| the rice transcription factor wrky53 suppresses herbivore-induced defenses by acting as a negative feedback modulator of mitogen-activated protein kinase activity. | the mechanisms by which herbivore-attacked plants activate their defenses are well studied. by contrast, little is known about the regulatory mechanisms that allow them to control their defensive investment and avoid a defensive overshoot. we characterized a rice (oryza sativa) wrky gene, oswrky53, whose expression is rapidly induced upon wounding and induced in a delayed fashion upon attack by the striped stem borer (ssb) chilo suppressalis. the transcript levels of oswrky53 are independent of ... | 2015 | 26453434 |
| geographic susceptibility of chilo suppressalis walker (lepidoptera: crambidae), to chlorantraniliprole in china. | chilo suppressalis walker, the rice striped stem borer (rssb) is one of the most serious lepidopteron insect pests of rice in asia. the registration of chlorantraniliprole added an option for the chemical control of rssbs. | 2014 | 24038844 |
| mating disruption or mass trapping, compared with chemical insecticides, for suppression of chilo suppressalis (lepidoptera: crambidae) in northeastern china. | asiatic rice borer, chilo suppressalis (walker), larvae cause extensive crop losses worldwide. because chemical control is problematic, and sex pheromone applications are a valuable management tactic in china, judicious timing of a minimal density of pheromone dispensers is important in developing a cost-effective c. suppressalis ipm program. during june-october in 2011, 20, 30, 40, and 50 dispensers per hectare for mass trapping, and 200, 300, 400, and 500 dispensers per hectare for mating disr ... | 2014 | 26309273 |
| immune response of chilo suppressalis walker (lepidoptera: crambidae) larvae to different entomopathogenic fungi. | the current study reports mortality and effects on cellular immune response of several entomopathogenic fungi including isoleates bb1, bb2 and bb3 of beauveria bassiana, metarhizium anisopliae, isaria fumosoroseus and lecanicilium lecanii against larvae of chilo suppressalis. prohemocytes, granulocytes, plasmatocytes and oenocytoids were identified as the main circulating hemocytes in the hemolymph of larvae using giemsa staining solution. entomopathogenic fungi caused differential mortality on ... | 2014 | 24447729 |
| prediction of pirnas using transposon interaction and a support vector machine. | piwi-interacting rnas (pirnas) are a class of small non-coding rna primarily expressed in germ cells that can silence transposons at the post-transcriptional level. accurate prediction of pirnas remains a significant challenge. | 2014 | 25547961 |
| spatial distribution and minimum sample size for overwintering larvae of the rice stem borer chilo suppressalis (walker) in paddy fields. | the rice stem borer, chilo suppressalis (walker), feeds almost exclusively in paddy fields in most regions of the world. the study of its spatial distribution is fundamental for designing correct control strategies, improving sampling procedures, and adopting precise agricultural techniques. field experiments were conducted during 2011 and 2012 to estimate the spatial distribution pattern of the overwintering larvae. data were analyzed using five distribution indices and two regression models (t ... | 2014 | 27193951 |
| characterization of cysteine protease-like genes in the striped rice stem borer, chilo suppressalis. | the striped rice stem borer, chilo suppressalis (walker), is a major pest for rice production in china and the rest of southeast asia. chemical control is the main means to alleviate losses due to this pest, which causes serious environmental pollution. an effective and environmentally friendly approach is needed for the management of the striped rice stem borer. cysteine proteases in insects could be useful targets for pest management either through engineering plant protease inhibitors, target ... | 2014 | 24702065 |
| amino acid substitutions of acetylcholinesterase associated with carbofuran resistance in chilo suppressalis. | over 1000-fold carbofuran resistance has been observed in chilo suppressalis (walker) collected from the changhua (ch) and chiayi (cy) prefectures of taiwan. an understanding of the pertinent mechanisms will benefit effective insecticide resistance management of c. suppressalis. | 2014 | 24616070 |
| omiga: optimized maker-based insect genome annotation. | insects are one of the largest classes of animals on earth and constitute more than half of all living species. the i5k initiative has begun sequencing of more than 5,000 insect genomes, which should greatly help in exploring insect resource and pest control. insect genome annotation remains challenging because many insects have high levels of heterozygosity. to improve the quality of insect genome annotation, we developed a pipeline, named optimized maker-based insect genome annotation (omiga), ... | 2014 | 24609470 |
| identification of candidate olfactory genes in chilo suppressalis by antennal transcriptome analysis. | antennal olfaction, which is extremely important for insect survival, mediates key behaviors such as host preference, mate choice, and oviposition site selection. in insects, odor detection is mediated by multiple proteins in the antenna, especially the odorant receptors (ors) and ionotropic receptors (irs), which ensure the specificity of the olfactory sensory neuron responses. in this study, we identified the olfactory gene repertoire of the rice stem borer, chilo suppressalis, an economically ... | 2014 | 25076861 |
| contrasting effects of ethylene biosynthesis on induced plant resistance against a chewing and a piercing-sucking herbivore in rice. | ethylene is a stress hormone with contrasting effects on herbivore resistance. however, it remains unknown whether these differences are plant- or herbivore-specific. we cloned a rice 1-aminocyclopropane-1-carboxylic acid (acc) synthase gene, osacs2, whose transcripts were rapidly up-regulated in response to mechanical wounding and infestation by two important pests: the striped stem borer (ssb) chilo suppressalis and the brown planthopper (bph) nilaparvata lugens. antisense expression of osacs2 ... | 2014 | 25064847 |
| characterization of the complete mitochondrial genome of chilo auricilius and comparison with three other rice stem borers. | the mitogenome of chilo auricilius (lepidoptera: pyraloidea: crambidae) was a circular molecule made up of 15,367 bp. sesamia inferens, chilo suppressalis, tryporyza incertulas, and c. auricilius, are closely related, well known rice stem borers that are widely distributed in the main rice-growing regions of china. the gene order and orientation of all four stem borers were similar to that of other insect mitogenomes. among the four stem borers, all at contents were below 83%, while all at conte ... | 2014 | 25042162 |
| susceptibility of the rice stem borer, chilo suppressalis (lepidoptera: crambidae), to flubendiamide in china. | the rice stem borer, chilo suppressalis (walker), is an important rice pest in china, and has evolved resistance to several classes of insecticides. flubendiamide is a phthalic diamide insecticide that shows selective insecticidal activity against lepidopterous insects. the susceptibility of 40 field populations of c. suppressalis, collected in 2011 and 2012 in seven provinces of south-eastern china, to flubendiamide was determined through rice seedling dipping bioassay method. of these 40 popul ... | 2014 | 25026690 |
| effect of synergists on susceptibility to chlorantraniliprole in field populations of chilo suppressalis (lepidoptera: pyralidae). | the asiatice stem borer, chilo suppressalis (walker), an important rice insect pest in china, has developed resistances to several classes of insecticides. to control c. suppressalis, chlorantraniliprole has been introduced as a novel insecticide in rice field since 2008. it is an anthranilic diamide insecticide that binds and activates ryanodine receptors (ryr). the susceptibility of field populations of c. suppressalis to chlorantraniliprole was determined in this study. the hypotheses of equa ... | 2014 | 24772562 |
| evaluation for potential trichogramma (hymenoptera: trichogrammatidae) strains for control of the striped stem borer (lepidoptera: crambidae) in the greater mekong subregion. | trichogramma species and strains differ significantly in host specificity and performance. nine trichogramma strains, six of them collected from paddy fields in the greater mekong subregion, were evaluated for performance on eggs of the striped stem borer, chilo suppressalis (walker), in both laboratory and field tests to determine potential trichogramma strains that can be used in an inundative release in an integrated pest management program. in the laboratory glass vial tests, all strains sho ... | 2014 | 25026653 |
| chilodb: a genomic and transcriptome database for an important rice insect pest chilo suppressalis. | chilodb is an integrated resource that will be of use to the rice stem borer research community. the rice striped stem borer (ssb), chilo suppressalis walker, is a major rice pest that causes severe yield losses in most rice-producing countries. a draft genome of this insect is available. the aims of chilodb are (i) to store recently acquired genomic sequence and transcriptome data and integrate them with protein-coding genes, micrornas, piwi-interacting rnas (pirnas) and rna sequencing (rna-seq ... | 2014 | 24997141 |
| changes in insecticide resistance of the rice striped stem borer (lepidoptera: crambidae). | application of insecticides is the most important method to control chilo suppressalis (walker) (lepidoptera: crambidae), and continuous use of individual insecticides has driven the rapid development of insecticide resistance in c. suppressalis during the past 30 yr. monitoring insecticide resistance provides information essential for integrated pest management. insecticide resistance of field populations to monosultap, triazophos, chlorpyrifos, and abamectin in china was examined in 2010 and 2 ... | 2014 | 24665718 |
| development and characterization of microsatellite markers for rice leaffolder, cnaphalocrocis medinalis (guenée) and cross-species amplification in other pyralididae. | rice leaffolder, cnaphalocrocis medinalis (guenée), is a destructive and widespread pest on rice. in this study, 20 microsatellite markers were isolated and characterized from c. medinalis partial genomic libraries using the method of fast isolation by aflp of sequence containing repeats. of these markers, 18 markers displayed polymorphisms. polymorphisms were evaluated in 48 individuals from two natural populations. the number of alleles per locus ranged from 2 to 15, and the expected and obser ... | 2014 | 24381106 |
| genome-wide analysis reveals the expansion of cytochrome p450 genes associated with xenobiotic metabolism in rice striped stem borer, chilo suppressalis. | the cytochrome p450 (cyp) superfamily is a large group of ancient proteins with enzymatic activities involved in various physiological processes. the rice striped stem borer, chilo suppressalis, is an important insect pest in rice production. here, we report the identification and characterization of 77 cyp genes from rice striped stem borer (ssb) through genome and transcriptome sequence analyses. all these cyp genes were confirmed by rt-pcr and direct sequencing. twenty-eight cyp transcripts h ... | 2014 | 24361403 |
| expression of cry1ab protein in a marker-free transgenic bt rice line and its efficacy in controlling a target pest, chilo suppressalis (lepidoptera: crambidae). | a marker-free bt transgenic rice line, mfb-mh86, was recently developed in china, which contains a cry1ab gene driven by a ubiquitin promoter. this bt gene confers resistance to a range of lepidopteran species, including the striped stem borer, chilo suppressalis (walker). the expression of cry1ab protein in mfb-mh86 leaves, stems and leaf sheaths (hereinafter referred to as stems), and roots was evaluated throughout the rice-growing season using an enzyme-linked immunosorbent assay. in addition ... | 2014 | 24495566 |
| the 9-lipoxygenase osr9-lox1 interacts with the 13-lipoxygenase-mediated pathway to regulate resistance to chewing and piercing-sucking herbivores in rice. | oxylipins produced by the 13-lipoxygenase (lox) have been reported to play an important role in plant defense responses to herbivores. yet, the role of oxylipins produced by the 9-lox pathway in this process remains largely unknown. here we cloned a gene encoding a chloroplast-localized 9-lox, osr9-lox1, from rice. transcriptional analysis revealed that herbivore infestation, mechanical wounding and jasmonic acid (ja) treatment either repressed or did not enhance the level of osr9-lox1 transcrip ... | 2014 | 24410960 |
| two splicing variants of a novel family of octopamine receptors with different signaling properties. | the octopamine and tyramine, as the invertebrate counterparts of the vertebrate adrenergic transmitters, control and modulate many physiological and behavioral processes. both molecules mediate their effects by binding to specific receptors belonging to the superfamily of g-protein-coupled receptors. so far, four families of octopamine and tyramine receptors have been reported. here, we described the functional characterization of one putative octopamine/tyramine receptor gene from the rice stem ... | 2014 | 24279508 |
| creation of bt rice expressing a fusion protein of cry1ac and cry1i-like using a green tissue-specific promoter. | the insecticidal genes from bacillus thuringiensis berliner (bt) have long been successfully used for development of insect-resistant rice. however, commercial planting of bt rice has been delayed by the concern over food safety, although no scientific evidence is ever found to justify the concern. to address this safety concern, we developed a transgenic insect-resistant rice line using a green tissue promoter to minimize the bt protein expression in the rice seeds. the bt protein expressed in ... | 2014 | 25195461 |
| binding site concentration explains the differential susceptibility of chilo suppressalis and sesamia inferens to cry1a-producing rice. | chilo suppressalis and sesamia inferens are two important lepidopteran rice pests that occur concurrently during outbreaks in paddy fields in the main rice-growing areas of china. previous and current field tests demonstrate that the transgenic rice line huahui 1 (hh1) producing a cry1ab-cry1ac hybrid toxin from the bacterium bacillus thuringiensis reduces egg and larval densities of c. suppressalis but not of s. inferens. this differential susceptibility to hh1 rice correlates with the reduced ... | 2014 | 24928872 |
| molecular characterization of the piggybac-like element, a candidate marker for phylogenetic research of chilo suppressalis (walker) in china. | transposable elements (tes, transposons) are mobile genetic dna sequences. tes can insert copies of themselves into new genomic locations and they have the capacity to multiply. therefore, tes have been crucial in the shaping of hosts' current genomes. tes can be utilized as genetic markers to study population genetic diversity. the rice stem borer chilo suppressalis walker is one of the most important insect pests of many subtropical and tropical paddy fields. this insect occurs in all the rice ... | 2014 | 25515331 |
| inducible expression of a fusion gene encoding two proteinase inhibitors leads to insect and pathogen resistance in transgenic rice. | plant proteinase inhibitors (pis) are considered as candidates for increased insect resistance in transgenic plants. insect adaptation to pi ingestion might, however, compromise the benefits received by transgenic expression of pis. in this study, the maize proteinase inhibitor (mpi), an inhibitor of insect serine proteinases, and the potato carboxypeptidase inhibitor (pci) were fused into a single open reading frame and introduced into rice plants. the two pis were linked using either the proce ... | 2014 | 24237606 |
| five small heat shock protein genes from chilo suppressalis: characteristics of gene, genomic organization, structural analysis, and transcription profiles. | small heat shock proteins (shsps) are the most diverse but also the most poorly known family of molecular chaperones, and they play essential roles in various biological processes. the striped stem borer, chilo suppressalis (insecta: lepidoptera: pyralidae), is one of the most serious pests of rice, causing extensive damage and yield loss. in this study, we isolated and characterized five members of the shsps family-cshsp19.8, cshsp21.4, cshsp21.5, cshsp21.7a, and cshsp21.7b-from c. suppressalis ... | 2013 | 23702967 |
| an improved pcr-restriction fragment length polymorphism (rflp) method for the identification of cry1-type genes. | the cry1-type genes of bacillus thuringiensis represent the largest cry gene family, which contains 50 distinct holotypes. it is becoming more and more difficult to identify cry1-type genes using current methods because of the increasing number of cry1-type genes. in the present study, an improved pcr-restriction fragment length polymorphism (pcr-rflp) method which can distinguish 41 holotypes of cry1-type genes was developed. this improved method was used to identify cry1-type genes in 20 b. th ... | 2013 | 23995930 |
| molecular cloning and characterization of the first caspase in the striped stem borer, chilo suppressalis. | apoptosis is executed through the activity of the caspases that are aspartyl-specific proteases. in this study, we isolated the caspase gene (cscaspase-1) of chilo suppressalis (one of the leading pests responsible for destruction of rice crops). it possesses the open reading frame (orf) of 295 amino acids including prodomain, large subunit and small subunits, and two cleavage sites (asp23 and asp194) were found to be located among them. in addition to these profiles, cscaspase-1 contains two ac ... | 2013 | 23676354 |
| parasitization by cotesia chilonis influences gene expression in fatbody and hemocytes of chilo suppressalis. | during oviposition many parasitoid wasps inject various factors, such as polydnaviruses (pdvs), along with eggs that manipulate the physiology and development of their hosts. these manipulations are thought to benefit the parasites. however, the detailed mechanisms of insect host-parasitoid interactions are not fully understood at the molecular level. based on recent findings that some parasitoids influence gene expression in their hosts, we posed the hypothesis that parasitization by a braconid ... | 2013 | 24086331 |
| diapause, signal and molecular characteristics of overwintering chilo suppressalis (insecta: lepidoptera: pyralidae). | diapause is a complex and dynamic process. chilo suppressalis, an important rice pest in asia enters facultative diapause as larvae. our results demonstrated in yangzhou, china, diapause was initiated between september 4 and 12, 2010. after diapause termination, c. suppressalis remained in quiescence in the field for as long as three months. the average time between collection of field larvae of c. suppressalis and their pupation decreased as the season progressed from fall to next spring. unexp ... | 2013 | 24226906 |
| susceptibility baseline and chlorantraniliprole resistance monitoring in chilo suppressalis (lepidoptera: pyralidae). | chlorantraniliprole, a new anthranilic diamide insecticide, has been commercialized in china since 2008 for controlling of several lepidopterans, including rice stem borer, chilo suppressalis walker. chemical control of this pest has become difficult because of its resistance development to many conventional insecticides. to facilitate chlorantraniliprole-resistance monitoring, seedling dip bioassays were conducted in 2011 and 2012 to assess the susceptibility of 30 populations of c. suppressali ... | 2013 | 24224264 |
| bt rice expressing cry2aa does not cause direct detrimental effects on larvae of chrysoperla sinica. | to assess the potential effects of cry2aa-expressing insect-resistant bt rice on chrysoperla sinica larvae, we conducted two tritrophic bioassays using a non-target (laodelphax striatellus) and a target herbivore (chilo suppressalis) as prey. none of the tested life-table parameters of c. sinica did differ when fed with l. striatellus nymphs reared on either bt or control rice plants. similarly, c. sinica larval survival and development were not affected when fed c. suppressalis larvae that were ... | 2013 | 24057602 |
| osmpk3 positively regulates the ja signaling pathway and plant resistance to a chewing herbivore in rice. | key message : silencing osmpk3 decreased elicited ja levels, which subsequently reduced levels of herbivore-induced trypsin protease inhibitors (tryppis) and improved the performance of ssb larvae, but did not influence bph. mitogen-activated protein kinases (mpks) are known to play an important role in plant defense by transferring biotic and abiotic signals into programmed cellular responses. however, their functions in the herbivore-induced defense response in rice remain largely unknown. her ... | 2013 | 23344857 |
| molecular cloning and pharmacological characterisation of a tyramine receptor from the rice stem borer, chilo suppressalis (walker). | tyramine (ta) and octopamine (oa) are considered to be the invertebrate counterparts of the vertebrate adrenergic transmitters. because these two phenolamines are the only biogenic amines whose physiological significance is presumably restricted to invertebrates, the attention of pharmacologists has been focused on the corresponding receptors, which are believed to represent promising targets for novel insecticides. for example, the formamidine pesticides, such as chlordimeform and amitraz, have ... | 2013 | 23129510 |
| mechanism and capacities of reducing ecological cost through rice-duck cultivation. | rice-duck cultivation is the essence of chinese traditional agriculture. a scientific assessment of the mechanism and its capacity is of theoretical significance and practical value in improving modern agricultural technology. | 2013 | 23703299 |
| regression analysis of dynamics of insecticide resistance in field populations of chilo suppressalis (lepidoptera: crambidae) during 2002-2011 in china. | to understand the evolution of insecticide resistance in the asiatic rice borer, chilo suppressalis (walker) (lepidoptera: crambidae), in field, regression analysis based on a linear or nonlinear model was adopted for analyzing resistance dynamics to six insecticides of two field populations of the lianyungang (lyg) and ruian (ra) populations during 2002-2011. for the low-level resistance population, lyg population, sustained susceptibilities to abamectin and fipronil were seen for 10 yr; a poly ... | 2013 | 24020300 |
| cloning and characterization of serpin-like genes from the striped rice stem borer, chilo suppressalis. | serpins, also called serine proteinase inhibitors, are widely distributed in eukaryotes. in insects, serpins play important roles in regulating immune responses, gut physiology, and other processes. here, we report the cloning and characterization of 12 serpin-like cdnas from the striped rice stem borer (chilo suppressalis), a major rice pest. the putative proteins share significant sequence similarity with known insect serpins, especially those from lepidopterons. analysis of functional domains ... | 2013 | 23957676 |
| [detection and analysis of symbiotic bacteria, arsenophonus and wolbachia, in striped stem borer, chilo suppressalis (lepidoptera: crambidae)]. | in this study, 23s rdna from arsenophonus and wsp gene from wolbachia were amplified by pcr method using specific primers to study their symbiosis in chilo suppressalis from 20 locations of china. the results indicated that arsenophonus and wolbachia infection rates differed widely in c. suppressalis populations. the infection rates of arsenophonus ranged from 5.0% to 50.0% in five geographical populations from harbin, huishui, jilin, nanyang, and yangzhou. wolbachia ranged from 25.0% to 40.0% i ... | 2013 | 24697073 |
| cloning, functional characterization, and expression profiles of nadph-cytochrome p450 reductase gene from the asiatic rice striped stem borer, chilo suppressalis (lepidoptera: pyralidae). | nadph-cytochrome p450 reductase (cpr) is one of the most important components of the cytochrome p450 enzyme system. it catalyzes electron transfer from nadph to all known p450s, thus plays central roles not only in the metabolism of exogenous xenobiotics but also in the regulation of endogenous hormones in insects. in this study, a full-length cdna encoding of a cpr (named cscpr) was isolated from the asiatic rice striped stem borer, chilo suppressalis, by using reverse transcription-polymerase ... | 2013 | 24036507 |
| amplifying long transcripts of ryanodine receptors of five agricultural pests by transcriptome analysis and gap filling. | ryanodine receptor (ryr) is an intracellular calcium release channel that plays a key role in excitation contraction coupling. insect ryr is the target of diamide insecticides. better understanding of insect ryr is necessary for studying the molecular mode of action and potential resistance mechanism of diamide insecticides. however, molecular manipulation of the full ryr gene is difficult because of its length (approximately 15 kb). at present, ryr genes have been reported only in a limited num ... | 2013 | 24299104 |
| osnpr1 negatively regulates herbivore-induced ja and ethylene signaling and plant resistance to a chewing herbivore in rice. | npr1 (a non-expressor of pathogenesis-related genes1) has been reported to play an important role in plant defense by regulating signaling pathways. however, little to nothing is known about its function in herbivore-induced defense in monocot plants. here, using suppressive substrate hybridization, we identified a npr1 gene from rice, osnpr1, and found that its expression levels were upregulated in response to infestation by the rice striped stem borer (ssb) chilo suppressalis and rice leaf fol ... | 2013 | 22694163 |