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two antimicrobial peptides from skin secretions of rana grahami.two antimicrobial peptides manifested a broad spectrum of antimicrobial activity against various microorganisms have been isolated from skin secretions of rana grahami. these antimicrobial peptides were named grahamin 1 and grahamin 2. their primary structures are gllsgilgagknivcglsglc and gllsgilgagkhivcglsglc, respectively, determined by edman degradation and mass spectrometry. they are structurally related to nigrocins identified from skin secretions of the dark-spotted frog, rana nigromacula ...200616487561
antimicrobial peptides from diverse families isolated from the skin of the asian frog, rana grahami.seven peptides with antimicrobial activity were isolated in pure form from an extract of the skin of the yunnanfu kunming frog rana grahami boulenger, 1917. the peptides were identified as belonging to the nigrocin-2 (three peptides), brevinin-1 (one peptide), brevinin-2 (three peptides), and esculentin-1 (one peptide) families. nigrocin-2grb (glfgkilgvgkkvlcglsgmc) containing three lysine residues, represented the peptide with highest potency against microorganisms (mic = 3 microm against esche ...200616621155
amphibian tachykinin precursor.the precursor of amphibian tachykinin has not been found although more than 30 tachykinins have been isolated from amphibians since 1964. in this report, two tachykinin-like peptides are identified from the skin secretions of the frog, odorrana grahami. their amino acid sequences are ddtedlankfiglm-nh(2) (named tachykinin og1) and ddasdrakkfyglm-nh(2) that is the same with ranamargarin found in rana margaretae, respectively, with a conserved fxglm-nh(2) c-terminal consensus motif. by cdna clonin ...200617045570
multiple bombesin-like peptides with opposite functions from skin of odorrana grahami.bombesin-like peptides (blps) are a family of neuroendocrinic peptides that mediate a variety of biological activities. three mature blps from the skin secretions of the frog odorrana grahami were purified. several bombesin-like peptide cdna sequences encoding precursors of blps were identified from the skin cdna library of o. grahami. this is the maximal diversity of blps ever found in animals. five mature blps (b1-b5) based on the amino acid sequences derived from the cdna cloning were synthes ...200717204393
anti-infection peptidomics of amphibian skin.peptidomics and genomics analyses were used to study an anti-infection array of peptides of amphibian skin. 372 cdna sequences of antimicrobial peptides were characterized from a single individual skin of the frog odorrana grahami that encode 107 novel antimicrobial peptides. this contribution almost triples the number of currently reported amphibian antimicrobial peptides. the peptides could be organized into 30 divergent groups, including 24 novel groups. the diversity in peptide coding cdna s ...200717272268
an antimicrobial peptide with antimicrobial activity against helicobacter pylori.an antimicrobial peptide named odorranain-hp was identified from skin secretions of the diskless odorous frog, odorrana grahami. it is composed of 23 amino acids with an amino acid sequence of gllrassvwgrkyyvdlagcaka. by blast search, odorranain-hp had similarity to antimicrobial peptide odorranain-w1 but it has a different gllr n-terminus. the cdna encoding odorranain-hp was cloned from the cdna library of the skin of o. grahami. this peptide showed antimicrobial activities against tested micro ...200717698252
a serine proteinase inhibitor from frog eggs with bacteriostatic activity.by sephadex g-50 gel filtration, resource q anionic exchange and c4 reversed phase liquid high performance liquid chromatography, a proteinase inhibitor protein (ranaserpin) was identified and purified from the eggs of the odour frog, rana grahami. the protein displayed a single band adjacent to the molecular weight marker of 14.4 kda analyzed by sds-page. the inhibitor protein homogeneity and its molecular weight were confirmed again by maldi-tof mass spectrometry analysis. the maldi-tof mass s ...200817826205
a novel antimicrobial peptide from amphibian skin secretions of odorrana grahami.a novel antimicrobial peptide named odorranain-nr was identified from skin secretions of the diskless odorous frog, odorrana grahami. it is composed of 23 amino acids with an amino acid sequence of gllsgilgagkhivcgltgcaka. odorranain-nr was classified into a novel family of antimicrobial peptide although it shared similarity with amphibian antimicrobial peptide family of nigrocin. odorranain-nr has an unusual intramolecular disulfide-bridged hexapeptide segment that is different from the intramo ...200818282640
a small trypsin inhibitor from the frog of odorrana grahami.a novel peptide inhibitor (ogti) of serine protease with a molecular weight of 1949.8, was purified from the skin secretion of the frog, odorrana grahami. of the tested serine proteases, ogti only inhibited the hydrolysis activity of trypsin on synthetic chromogenic substrate. this precursor deduced from the cdna sequence is composed of 70 amino acid residues. the mature ogti contains 17 amino acid residues including a six-residue loop disulfided by two half-cysteines (avnipfkvhfrckaafc). in add ...200818472011
odorranalectin is a small peptide lectin with potential for drug delivery and targeting.lectins are sugar-binding proteins that specifically recognize sugar complexes. based on the specificity of protein-sugar interactions, different lectins could be used as carrier molecules to target drugs specifically to different cells which express different glycan arrays. in spite of lectin's interesting biological potential for drug targeting and delivery, a potential disadvantage of natural lectins may be large size molecules that results in immunogenicity and toxicity. smaller peptides whi ...200818584053
kassina senegalensis skin tachykinins: molecular cloning of kassinin and (thr2, ile9)-kassinin biosynthetic precursor cdnas and comparative bioactivity of mature tachykinins on the smooth muscle of rat urinary bladder.tachykinins are among the most widely-studied families of regulatory peptides characterized by a highly-conserved c-terminal -phe-x-gly-leu-met.amide motif, which also constitutes the essential bioactive core. the amphibian skin has proved to be a rich source of these peptides with physalaemin from the skin of physalaemus fuscomaculatus representing the archetypal aromatic tachykinin (x=tyr or phe) and kassinin from the skin of kassina senegalensis representing the archetypal aliphatic tachykini ...200919298842
combined peptidomics and genomics approach to the isolation of amphibian antimicrobial peptides.a large number of diverse antimicrobial peptides have been found in amphibian skins, and many more remain to be identified. it is sufficiently easy to obtain amounts of gland secretions sufficient for both identification and functional testing of the bioactive peptides. we describe here a systematic peptidomics approach which we combined with genomics and functional testing. this has proven to be an effective way to identify amphibian antimicrobial peptides, including novel peptide families. pro ...201020013209
cloning and characterization of the first amphibian bradykinin gene.more than ten bradykinin-related peptides and their cdnas have been identified from amphibians, but their genes are unknown. in present study, four cdnas encoding one, two, four and six copies of bradykinin-related peptides were cloned from the frog (odorrana grahami) skin cdna library, respectively. three bradykinin-related peptides (bradykinin, thr6-bradykinin, leu5thr6-bradykinin) were deduced from these four cdna sequences. based on the cdna sequence, the gene sequence encoding an amphibian ...201020025925
[in vitro activities of odorrana grahami antimicrobial peptides from skin against 5 pathogenic bacteria].to evaluate the efficiency of 4 odorrana grahami antimicrobial peptides from skin against 5 pathogenic bacteria, include 2 wild strains and 7 resistant strains.200920432894
identification and characterization of antimicrobial peptides from the skin of the endangered frog odorrana ishikawae.the endangered anuran species, odorrana ishikawae, is endemic to only two small japanese islands, amami and okinawa. to assess the innate immune system in this frog, we investigated antimicrobial peptides in the skin using artificially bred animals. nine novel antimicrobial peptides containing the c-terminal cyclic heptapeptide domain were isolated on the basis of antimicrobial activity against escherichia coli. the peptides were members of the esculentin-1 (two peptides), esculentin-2 (one pept ...201021193000
gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, odorrana grahami.amphibian opiate peptides including dermorphins and deltorpins have been recently found only in the skin of south american frogs belonging to the subfamily phyllomedusinae (phyllomedusa, agalychnis and pachymedusa species). no opiate peptides have ever been identified from other amphibians or organs except skin. here we report the purification and characterization of a novel antinociceptive peptide named odorranaopin from the homogenates of the frog brains, odorrana grahami, which is also the fi ...201121440596
identification and structure-activity relationship of an antimicrobial peptide of the palustrin-2 family isolated from the skin of the endangered frog odorrana ishikawae.recently, we identified nine novel antimicrobial peptides from the skin of the endangered anuran species, odorrana ishikawae, to assess its innate immune system. in this study an additional antimicrobial peptide was initially isolated based on antimicrobial activity against escherichia coli. the new antimicrobial peptide belonging to the palustrin-2 family was named palustrin-2isb. it consists of 36 amino acid residues including 7 amino acids c-terminal to the cyclic heptapeptide rana box domain ...201121911019
bi-functional peptides with both trypsin-inhibitory and antimicrobial activities are frequent defensive molecules in ranidae amphibian skins.amphibian skins act as the first line against noxious aggression by microorganisms, parasites, and predators. anti-microorganism activity is an important task of amphibian skins. a large amount of gene-encoded antimicrobial peptides (amps) has been identified from amphibian skins. only a few of small protease inhibitors have been found in amphibian skins. from skin secretions of 5 species (odorrana livida, hylarana nigrovittata, limnonectes kuhlii, odorrana grahami, and amolops loloensis) of ran ...201221927839
effects of thioredoxin: sumo and intein on soluble fusion expression of an antimicrobial peptide og2 in escherichia coli.og2 is a modified antimicrobial peptide of palustrin-og1 (og1), which is derived from odorrana grahami frog. og2 has shown much higher selective antimicrobial activity and lower hemolytic activity than og1, indicating og2 may be a promising antimicrobial agent. in this study, we investigated three fusion partners, including thioredoxin, mxe gyra intein, and small ubiquitin-like modifier (sumo), each fused with og2, and examined their effects on the expression level and solubility of og2 in esche ...201322670762
effects of amino acid deletion and substitution on the chemical properties, biological activities of the frog peptide palustrin-og1.palustrin-og1 (og1) is a host defense peptide isolated from the frog odorrana grahami. in this study, we analyzed the chemical properties, antimicrobial activities and cytotoxicities of og1 and its derivatives to identify the most promising peptide as an antimicrobial agent. by increasing the net positive charge, amphipathicity and decreasing the mean hydrophobicity of og1, the derivative named as og2 exerted higher antimicrobial activity against bacteria but lower cytotoxicity against both porc ...201323061668
a potential wound healing-promoting peptide from frog skin.cutaneous wound healing is a dynamic, complex, and well-organized process that requires the orchestration of many different cell types and cellular processes. transforming growth factor β1 is an important factor that plays a key role during wound healing. amphibian skin has been proven to possess excellent wound healing ability, whilst no bioactive substrate related to it has ever been identified. here, a potential wound healing-promoting peptide (ah90, atawdfgphgllpirpirirplcg) was identified f ...201424441016
a short peptide from frog skin accelerates diabetic wound healing.delayed wound healing will result in the development of chronic wounds in some diseases, such as diabetes. amphibian skins possess excellent wound-healing ability and represent a resource for prospective wound-healing promoting compounds. a potential wound-healing promoting peptide (cw49; amino acid sequence apfrmgicttn) was identified from the frog skin of odorrana grahami. it promotes wound healing in a murine model with a full-thickness dermal wound in both normal and diabetic animals. in add ...201425117795
atomic resolution crystal structure of hv-bbi protease inhibitor from amphibian skin in complex with bovine trypsin.protease inhibitors of the bowman-birk (bbi) family are commonly found in plants and animals where they play a protective role against invading pathogens. here, we report an atomic resolution (1å) crystal structure of a peptide inhibitor isolated from a skin secretion of a chinese bamboo odorous frog huia versabilis (hv-bbi) in complex with trypsin. hv-bbi shares significant similarities in sequence with a previously described inhibitor from a diskless-fingered odorous frog odorrana graham (orb) ...201525546528
targeting cancer-specific glycans by cyclic peptide lectinomimics.the transformation from normal to malignant phenotype in human cancers is associated with aberrant cell-surface glycosylation. thus, targeting glycosylation changes in cancer is likely to provide not only better insight into the roles of carbohydrates in biological systems, but also facilitate the development of new molecular probes for bioanalytical and biomedical applications. in the reported study, we have synthesized lectinomimics based on odorranalectin 1; the smallest lectin-like cyclic pe ...201728894966
skin innate immunity of diskless-fingered odorous frogs (odorrana grahami) with spatial-temporal variations.the skin innate immunities of diskless-fingered odorous frogs (odorrana grahami) from three populations were investigated. the antimicrobial capacities of skin secretions against the 60 representative environmental bacterial strains were evaluated using the values of the minimum inhibitory concentration (mic) equivalents, which were defined as the volumes of antimicrobial solution just inhibiting the tested bacteria per 1 cm2 of surface area, from 0.06 to 9.10 ml/cm2. our results revealed signif ...201830092315
identification of peptides implicated in antibacterial activity of snow crab hepatopancreas hydrolysates by a bioassay-guided fractionation approach combined with mass spectrometry.snow crab (chionoecetes opilio) by-products are a rich source of biomolecules, such as lipids, proteins, and chitin, which have not been extensively investigated. this study aims to identify antibacterial peptides to enhance the value of c. opilio by-products. after hydrolysis of different component parts using protamex®, and concentration by solid-phase extraction, the resulting fractions were tested for antibacterial activity against escherichia coli, listeria innocua, and vibrio parahaemolyti ...201930443874
bacterial community analysis on the skin of odorrana grahami and proposal of comamonas aquatica subsp. aquatica subsp. nov. and comamonas aquatica subsp. rana subsp. nov.several studies indicated that odorrana grahami (o. grahami) skin contains abundant antimicrobial peptides, and the skin was recognized as hostile habitat for microorganisms. in this study, the microbial community was evaluated by 16s rrna gene sequencing, and two associated bacterial isolates were obtained and characterized from the skin of o. grahami. sixteen bacterial genera were identified from the o. grahami skin by uncultured clone sequences. the dominant groups were comamonas, bacillus an ...201930783796
a hylarana latouchii skin secretion-derived novel bombesin-related pentadecapeptide (ranatensin-hla) evoke myotropic effects on the in vitro rat smooth muscles.amphibians have developed successful defensive strategies for combating predators and invasive microorganisms encountered in their broad range of environments, which involve secretion of complex cocktails of noxious, toxic and diverse bioactive molecules from the skins. in recent years, amphibian skin secretions have been considered as an extraordinary warehouse for the discovery of therapeutic medicines. in this study, through bioactivity screening of the hylarana latouchii skin secretion-deriv ...201930959738
ranacyclin-nf, a novel bowman-birk type protease inhibitor from the skin secretion of the east asian frog, pelophylax nigromaculatus.serine protease inhibitors are found in plants, animals and microorganisms, where they play important roles in many physiological and pathological processes. inhibitor scaffolds based on natural proteins and peptides have gradually become the focus of current research as they tend to bind to their targets with greater specificity than small molecules. in this report, a novel bowman-birk type inhibitor, named ranacyclin-nf (rnf), is described and was identified in the skin secretion of the east a ...202032630758
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