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Characterization of ß-galactoside phosphorylases with diverging acceptor specificities.Glycoside phosphorylases are a special group of carbohydrate-active enzymes, with characteristics in between those of glycoside hydrolases and glycosyl transferases. The phosphorylases from family GH-112 are exceptional because they employ galactose-1-phosphate instead of glucose-1-phosphate as glycosyl donor. Different acceptor specificities have been observed in this family, ranging from l-rhamnose to GlcNAc, GalNAc and a combination of the latter. Three new phosphorylases from previously unex ...201122112272
burden of emerging anaerobes in the maldi-tof and 16s rrna gene sequencing era.the isolation of anaerobes from patients has declined in recent years, whereas their detection by molecular techniques has increased. in the present work, we analyzed the application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequencing to routine identification of anaerobes in clinical microbiology laboratory. we identified 544 isolates of 79 species by routine culture from deep samples in our hospital. maldi-tof ms allowed i ...201121672636
complete genome sequence of anaerococcus prevotii type strain (pc1).anaerococcus prevotii (foubert and douglas 1948) ezaki et al. 2001 is the type species of the genus, and is of phylogenetic interest because of its arguable assignment to the provisionally arranged family 'peptostreptococcaceae'. a. prevotii is an obligate anaerobic coccus, usually arranged in clumps or tetrads. the strain, whose genome is described here, was originally isolated from human plasma; other strains of the species were also isolated from clinical specimen. here we describe the featur ...200921304652
identification of gram-positive anaerobic cocci by maldi-tof mass spectrometry.gram-positive anaerobic cocci (gpac) are part of the commensal microbiota of humans and are a phylogenetically heterogeneous group of organisms. to evaluate the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) for the identification of gpac, a database was constructed, using reference strains of commonly encountered gpac and clinical isolates of which the sequence of the 16s rrna gene was determined. subsequently, the database was validat ...201121292417
a comparison of bacterial composition in diabetic ulcers and contralateral intact skin.an extensive portion of the healthcare budget is allocated to chronic human infection. chronic wounds in particular are a major contributor to this financial burden. little is known about the types of bacteria which may contribute to the chronicity, biofilm and overall bioburden of the wound itself. in this study we compare the bacteriology of wounds and associated intact skin. wound and paired intact skin swabs (from a contralateral location) were collected. the bacterial diversity was determin ...201020461221
in vitro activity of tigecycline and comparators against a european compilation of anaerobes collected as part of the tigecycline evaluation and surveillance trial (test).the tigecycline evaluation and surveillance trial (test) is a global surveillance study designed to monitor the in vitro activity of the broad-spectrum antimicrobial tigecycline against nosocomial- and community-acquired pathogens. in this study the in vitro activity of tigecycline against 1256 anaerobic pathogens collected across europe has been compared to the activity of several comparator antibiotics. the pathogens examined in this study included bacteroides, prevotella, anaerococcus, clostr ...201019883155
[bacteria isolated from surgical infections and their susceptibility to antimicrobial agents--special reference to bacteria isolated between april 2007 and march 2008].bacteria isolated from infections in abdominal surgery during the period from april 2007 to march 2008 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 707 strains including 24 strains of candida spp. were isolated from 181 (79.0%) of 229 patients with surgical infections. three hundred and ninety-five strains were isolated from primary infections, and 288 strains were isolated from postoperative infections. from primary infections, anae ...200919860320
polymicrobial nature of chronic diabetic foot ulcer biofilm infections determined using bacterial tag encoded flx amplicon pyrosequencing (btefap).diabetic extremity ulcers are associated with chronic infections. such ulcer infections are too often followed by amputation because there is little or no understanding of the ecology of such infections or how to control or eliminate this type of chronic infection. a primary impediment to the healing of chronic wounds is biofilm phenotype infections. diabetic foot ulcers are the most common, disabling, and costly complications of diabetes. here we seek to derive a better understanding of the pol ...200818833331
comparison of the diversity of the vaginal microbiota in hiv-infected and hiv-uninfected women with or without bacterial vaginosis.whether human immunodeficiency virus (hiv) infection is associated with a change in the diversity of genital microbiota in women was investigated.200818717638
detection and identification of bacterial dna in semen.to detect and identify bacteria in semen by sequencing polymerase chain reaction (pcr)-amplified ribosomal rna gene regions (rdnas).200818191853
peptoniphilus gorbachii sp. nov., peptoniphilus olsenii sp. nov., and anaerococcus murdochii sp. nov. isolated from clinical specimens of human origin.three groups of previously unknown gram-positive, anaerobic, coccus-shaped bacteria were characterized using phenotypic and molecular taxonomic methods. phenotypic and genotypic data demonstrate that these organisms are distinct, and each group represents a previously unknown subline within clostridium cluster xiii. two groups are most closely related to peptoniphilus harei in the genus peptoniphilus, and the other group is most closely related to anaerococcus lactolyticus in the genus anaerococ ...200717428937
in vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections.against 443 aerobic and anaerobic bacteria isolated from diabetic foot infections, ceftobiprole mics (microg/ml) at which 90% of the isolates tested were inhibited were as follows: methicillin-resistant staphylococcus aureus, 1; methicillin-susceptible s. aureus and staphylococcus lugdunensis, 0.5; anaerococcus prevotii, 0.125; finegoldia magna, 0.5; peptoniphilus asaccharolyticus, 1; peptostreptococcus anaerobius, 4; escherichia coli and enterobacter species, 0.125; klebsiella species, 2; and p ...200616982780
in vitro activities of dalbavancin and 12 other agents against 329 aerobic and anaerobic gram-positive isolates recovered from diabetic foot infections.tests of dalbavancin's in vitro activity against 209 aerobic and 120 anaerobic isolates from pretreatment diabetic foot infections showed an mic(90) of < or =0.125 microg/ml against methicillin-susceptible staphylococcus aureus (mssa), methicillin-resistant s. aureus (mrsa), and 120 anaerobes (clostridium perfringens, other clostridia, peptoniphilus asaccharolyticus, finegoldia magna, and anaerococcus prevotii), compared to respective mic(90)s for mssa and mrsa of 0.5 and 1 microg/ml for vancomy ...200616870792
in vitro activity of oritavancin (ly333328), vancomycin, clindamycin, and metronidazole against clostridium perfringens, propionibacterium acnes, and anaerobic gram-positive cocci.using an agar dilution method, we determined the in vitro activity of oritavancin, vancomycin, clindamycin and metronidazole against 114 unique clinical isolates of gram-positive anaerobes. mic(90)s (microg/ml) for oritavancin were as follows: clostridium perfringens 1.0, propionibacterium acnes 0.25, peptostreptococcus anaerobius 0.25, peptoniphilus asaccharolyticus 0.5, finegoldia magna 0.25, micromonas. micros 0.25, and anaerococcus prevotii 0.25. on a weight basis, oritavancin is slightly mo ...200516701537
culture-independent identification of pathogenic bacteria and polymicrobial infections in the genitourinary tract of renal transplant recipients.renal transplant recipients are predisposed to urinary tract infections caused by both common uropathogens and opportunistic bacteria resulting frequently in significant polymicrobial infections. in this study, a culture-independent 16s rrna-based approach was established to identify unusual, fastidious, or anaerobic bacteria and to investigate bacterial diversity in urinary tract specimens. similarly sized amplicons encompassing the v6 to v8 region of the 16s rrna were analyzed with denaturing ...200314662931
heterogeneity within the gram-positive anaerobic cocci demonstrated by analysis of 16s-23s intergenic ribosomal rna polymorphisms.peptostreptococci are gram-positive, strictly anaerobic bacteria which, although regarded as members of the commensal human microflora, are also frequently isolated from sites of clinical infection. the study of this diverse group of opportunist pathogens has been hindered by an inadequate taxonomy and the lack of a valid identification scheme. recent re-classification of the peptostreptococcus family into five distinct genus groups has helped to clarify the situation. however, this has been on ...200212448679
The complex vaginal flora of West African women with bacterial vaginosis.The spectrum of bacteria associated with bacterial vaginosis (BV) has recently expanded through taxonomic changes and the use of molecular methods. These methods have yet to be used in large-scale epidemiological studies in Africa where BV is highly prevalent.201121949860
complete genome sequence of a serotype 11a, st62 streptococcus pneumoniae invasive isolate.streptococcus pneumoniae is an important human pathogen representing a major cause of morbidity and mortality worldwide. we sequenced the genome of a serotype 11a, st62 s. pneumoniae invasive isolate (ap200), that was erythromycin-resistant due to the presence of the erm(tr) determinant, and carried out analysis of the genome organization and comparison with other pneumococcal genomes.201121284853
the effects of circumcision on the penis microbiome.circumcision is associated with significant reductions in hiv, hsv-2 and hpv infections among men and significant reductions in bacterial vaginosis among their female partners.201020066050
development of 16s rrna-based probes for the identification of gram-positive anaerobic cocci isolated from human clinical specimens.fluorescent probes targeted at 16s rrna were designed for peptostreptococcus anaerobius and peptostreptococcus stomatis (pana134), parvimonas micra (pamic1435), finegoldia magna (fmag1250), peptoniphilus asaccharolyticus (pnasa1254), peptoniphilus ivorii (pnivo731), peptoniphilus harei (pnhar1466), anaerococcus vaginalis (avag1280) and anaerococcus lactolyticus (alac1438), based on the 16s rrna sequences of reference strains and 88 randomly chosen clinical isolates. these strains were also used ...200717714522
rapid identification of gram-positive anaerobic coccal species originally classified in the genus peptostreptococcus by multiplex pcr assays using genus- and species-specific primers.here, a rapid and reliable two-step multiplex pcr assay for identifying 14 gram-positive anaerobic cocci (gpac) species originally classified in the genus peptostreptococcus (anaerococcus hydrogenalis, anaerococcus lactolyticus, anaerococcus octavius, anaerococcus prevotii, anaerococcus tetradius, anaerococcus vaginalis, finegoldia magna, micromonas micros, peptostreptococcus anaerobius, peptoniphilus asaccharolyticus, peptoniphilus harei, peptoniphilus indolicus, peptoniphilus ivorii and pepton ...200312855723
proposal of the genera anaerococcus gen. nov., peptoniphilus gen. nov. and gallicola gen. nov. for members of the genus peptostreptococcus.members of genus peptostreptococcus have previously been found to be distantly related to the type species, peptostreptococcus anaerobius, on the basis of 16s rdna sequence similarities. they were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. the reclassification of the species of these three groups into three new genera, peptoniphilus gen. nov., anaerococcus gen. nov. and gallicola gen. nov., is proposed. the genu ...200111491354
septic arthritis in a native joint due to anaerococcus prevotii.a 37-year-old injecting drug user presented with signs and symptoms of septic arthritis. staphylococcus aureus was grown from his blood cultures. despite treatment with flucloxacillin and fusidic acid his condition continued to deteriorate. echocardiography showed no signs of endocarditis. culture of his knee aspirate grew anaerococcus prevotii after 5 days of incubation. metronidazole was added to his treatment regime. a collection of pus (800 ml) was drained from the right thigh and a prevotii ...200818344234
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