TitleAbstractYear(sorted descending)
crystal structure of the escherichia coli fic toxin-like protein in complex with its cognate antitoxin.fic domain proteins mediate post-translational modifications of target proteins, which typically results in their inactivation. depending on the conservation of crucial active site residues, the fic fold serves as structural scaffold for various enzymatic activities, mostly target adenylylation. the founding member of the vast fic protein family, ecfict, was identified in escherichia coli some time ago. the g55r point mutant of ecfict displays the "filamentation induced by camp" (fic) phenotype ...201627657533
molecular survey of anaplasma and ehrlichia of red deer and sika deer in gansu, china in 2013.anaplasma and ehrlichia are important emerging tick-borne pathogens in both humans and animals. here, we conducted a molecular surveillance study in gansu, china to assess the prevalence of anaplasma and ehrlichia spp. in red deer and sika deer based on polymerase chain reaction (pcr) analysis and sequencing of 16s rrna or msp genes. pcr revealed that the prevalence of anaplasma ovis, anaplasma bovis and anaplasma platys of the qilian mountain samples was 32%, 9% and 9%, respectively; the preval ...201625660960
vertical transmission of bartonella schoenbuchensis in lipoptena cervi.lipoptena cervi (diptera: hippoboscidae) is a hematophagous ectoparasite of cervids, which is considered to transmit pathogens between animals and occasionally to humans. the principal life stage that is able to parasitize new hosts is a winged ked that just emerged from a pupa. to facilitate efficient transmission of pathogens between hosts, vertical transmission from female deer keds to their offspring is necessary. we investigated vertical transmission of several vector-borne pathogens associ ...201525889985
conserved inhibitory mechanism and competent atp binding mode for adenylyltransferases with fic fold.the ubiquitous fic domain is evolutionarily conserved from bacteria to human and has been shown to catalyze amp transfer onto protein side-chain hydroxyl groups. recently, it was predicted that most catalytically competent fic proteins are inhibited by the presence of an inhibitory helix ╬▒inh that is provided by a cognate anti-toxin (class i), or is part of the n- or c-terminal part of the fic protein itself (classes ii and iii). in vitro, inhibition is relieved by mutation of a conserved glutam ...201323738009
molecular detection of bartonella grahamii and b. schoenbuchensis-related species in korean water deer (hydropotes inermis argyropus).we determined the prevalence of bartonella spp. and investigated which species of bartonella naturally infects korean water deer (kwd, hydropotes inermis argyropus) in the republic of korea (rok). a total of 70 spleens from kwd carcasses were collected by the conservation genome resource bank for korean wildlife (cgrb) in the rok between 2008 and 2009. nested pcrs were performed using the rpob gene and internal transcribed spacer (its) region primers to amplify the dna fragment of bartonella. us ...201323473217
adenylylation control by intra- or intermolecular active-site obstruction in fic proteins.fic proteins that are defined by the ubiquitous fic (filamentation induced by cyclic amp) domain are known to catalyse adenylylation (also called ampylation); that is, the transfer of amp onto a target protein. in mammalian cells, adenylylation of small gtpases through fic proteins injected by pathogenic bacteria can cause collapse of the actin cytoskeleton and cell death. it is unknown how this potentially deleterious adenylylation activity is regulated in the widespread fic proteins that are f ...201222266942
cloning, expression, purification, and biochemical characterisation of the fic motif containing protein of mycobacterium tuberculosis.the role of fic (filamentation induced by camp)(2) domain containing proteins in the regulation of many vital pathways, mostly through the transfer of nmps from ntps to specific target proteins (nmpylation), in microorganisms, higher eukaryotes, and plants is emerging. the identity and function of fic domain containing protein of the human pathogen, mycobacterium tuberculosis, remains unknown. in this regard, m. tuberculosis fic gene (mtfic) was cloned, overexpressed, and purified to homogeneity ...201222982230
phylogenetics and population genetics of the louse fly, lipoptena mazamae, from arkansas, usa.louse flies, also known as deer keds (lipoptena mazamae rondani), infest cervids such as white-tailed deer, odocoileus virginianus and vector pathogens such as anaplasma and bartonella schoenbuchensis to cattle and humans, respectively. the population genetic structure of 30 l. mazamae collected from white-tailed deer in four regions of arkansas, u.s.a., designated by county boundaries, was examined using dna sequences of a 259-bp region of the mitochondrial dna rrna 16s gene. of the 259 nucleot ...201020534010
molecular detection of bartonella schoenbuchensis from ectoparasites of deer in massachusetts.deer keds (lipoptena cervi) are thought to have been introduced into new england from europe during the 1800 s. we sought to determine whether l. cervi from massachusetts deer contained evidence of infection by bartonella schoenbuchensis, which appears to be maintained by l. cervi in europe. five of 6 keds were found to contain b. schoenbuchensis dna, and 2 deer ticks cofeeding on deer with such keds did as well. the detection of bartonella dna in deer ticks probably represents contamination by ...200818454595
bartonella spp. in deer keds, lipoptena mazamae (diptera: hippoboscidae), from georgia and south carolina, usa.deer keds, lipoptena mazamae (diptera: hippoboscidae), were collected from white-tailed deer (odocoileus virginianus) and humans in georgia and south carolina, usa (1 october 2001-6 january 2005) and screened for the presence of dna from bartonella spp. forty deer keds were screened for bartonella spp. by polymerase chain reaction using primers specific to the riboflavin synthase gene (ribc) of bartonella. bartonella species closely related to bartonella schoenbuchensis and to the etiologic agen ...200616870863
isolation of bartonella schoenbuchensis from lipoptena cervi, a blood-sucking arthropod causing deer ked dermatitis.bartonella schoenbuchensis, which commonly causes bacteremia in ruminants, was isolated from the deer ked lipoptena cervi and was shown to localize to the midgut of this blood-sucking arthropod, causing deer ked dermatitis in humans. the role of b. schoenbuchensis in the etiology of deer ked dermatitis should be further investigated.200415528732
bartonella schoenbuchensis isolated from the blood of a french cow. 200312860633
bartonella schoenbuchii sp. nov., isolated from the blood of wild roe deer.the genus bartonella comprises two human-specific pathogens and a growing number of zoonotic or animal-specific species. domesticated as well as wild mammals can serve as reservoir hosts for the zoonotic agents and transmission to humans may occur by blood sucking arthropods or by direct blood to blood contact. humans may come into intimate contact with free-ranging mammals during hunting, especially during evisceration with bare hands, when accidental blood to blood contact frequently occurs. t ...200111491358
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