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description of arthrobacter creatinolyticus sp. nov., isolated from human urine.three strains of creatinine-hydrolysing bacteria isolated from human urine were characterized taxonomically. they were aerobic, non-spore-forming, gram-positive rods with the peptidoglycan of the cell wall containing lysine. mk-8 and mk-9 were found to be the major types of menaquinone. the g + c content of the dna was 66-67 mol%. the 16s rrna sequence of one strain (gifu 12498) was determined and aligned with other high-g + c-content gram-positive rods from different genera. following phylogene ...19989731280
screening and production of a potent extracellular arthrobacter creatinolyticus urease for determination of heavy metal ions.this paper describes the isolation of a potent extracellular urease producing microorganism, identified by 16s rrna as arthrobacter creatinolyticus mtcc 5604 and its medium optimization by classical one-factor-at-a-time method and central composite rotatable design (ccrd), a tool of response surface methodology (rsm). an optimal activity of 9.0 u ml(-1) was obtained by classical method and statistical optimization of the medium resulted in an activity of 17.35 u ml(-1) at 48 h and 30 °c. this ac ...201423553897
potentiometric biosensor for determination of urea in milk using immobilized arthrobacter creatinolyticus urease.the extracellular urease from arthrobacter creatinolyticus was partially purified by ammonium sulfate precipitation and immobilized on pan [poly(acrylonitrile-methylmethacrylate-sodium vinylsulfonate)] membrane. the urease immobilized pan membrane exhibited an activity of 97.92u/cm(2) under the optimum conditions of 1.0% enzyme concentration, 15% glutaraldehyde, 24h immobilization time and temperature of 4°c. the changes in surface morphology of the membrane after immobilization were studied by ...201525687009
bacteremia due to arthrobacter creatinolyticus in an elderly diabetic man with acute cholangitis.an 87-year-old man with poorly controlled diabetic mellitus presented with fever, bedsores, and elevated hepatobiliary enzyme levels. he was diagnosed with bacteremia with acute cholangitis due to arthrobacter species, which are gram-positive, aerobic, catalase-positive, coryneform bacteria belonging to the family microbacteriaceae. doripenem and subsequencial sulbactam/ampicillin treatment were used for the acute cholangitis, and the bacteremia was treated with a 2-week course of vancomycin. th ...201727357988
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