PMID(sorted descending)
the structural gene for nadp l-glutamate dehydrogenase in aspergillus nidulans.a total of 41 mutants lacking nadp l-glutamate dehydrogenase (nadp-gdh) activity have been studied. all the mutations were located at the gdha locus within 0-1% recombination of gdhai. two mutants, gdhai and gdha2, out of five examined, produced cross-reacting material which neutralized nadp-gdh anti-serum. the mutant gdha9 has altered km values for all five substrates: ammonium, alpha-ketoglutarate, l-glutamate, nadph and nadp. the mutant gdha20 had temperature-sensitive growth, abnormal ammoni ...1975234511
the dark respiration of anacystis nidulans. production of hcn from histidine and oxidation of basic amino acids.the basic amino acids, l-arginine, l-lysine, lo-irnithine, and to a lesser extent l-histidine, strongly stimulate the o2 uptake of cell suspensions of the blue-green alga or cyanobacterium anacystis nidulans. in the case of l-histidine, the extra o2 consumption is associated with the formation in vivo of small amounts of hcn, particularly in an atmosphere of o2. the enzyme responsible for both the stimulated o2 uptake with the basic amino acids and the formation of hcn from histidine has been is ...1979223652
an iron-containing superoxide dismutase from anacystis nidulans.superoxide dismutase (sod) was isolated and purified from anacystis nidulans to near electrophoretic homogeneity. the enzyme has a molecular weight of 37,500, as determined by gel filtration and sds-gel electrophoresis. the enzyme molecule consists of two subunits of identical molecular weight. proton-induced x-ray elemental analysis (pixe) showed that the sod of a. nidulans is an iron-containing enzyme; the fe:enzyme mol ratio was found to be 1. the epr spectra indicated that the active center ...1979222743
effect of growth temperature on the cell constituents of aspergillus nidulans. 1977202564
the effect of glucose and manganese on adenosine-3',5'-monophosphate levels during growth and differentiation of aspergillus nidulans.the role of cyclic adenosine monophosphate (camp) during growth and development of aspergillus nidulans was investigated. in normal cultures the highest amount of camp, expressed on a dry weight basis, was found after 24 h of growth when still more than 5% glucose was present in the medium. after depletion of the medium even a slight fall in camp was noted. glucose concentrations ranging from 0.5-12% resulted in a slight decrease in the amount of camp as measured after 24 h of growth. cultures w ...1976179489
three genes determine the carboxin sensitivity of mitochondrial succinate oxidation in aspergillus nidulans. 1975178574
formation in the dark, of virus-induced deoxyribonuclease activity in anacystis nidulans, an obligate anacystis nidulans, upon infection with cyanophage as-1, after a lag period of 1 h the level of deoxyribonuclease (dnase) activity increaded rapidly up to 15- to 20-fold in 4 to 5 h in the light. in contrast, the ribonuclease and phosphomonoesterase activities increased significantly only 4 to 5 h after infection, i.e. as late as 1 h prior to lysis. in complete darkness, the nuclease levels remained unaltered. however, when the infected cells were exposed to light for 1 or 2 h after infection ...1976177400
mutants of aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase.ten mutants of aspergillus nidulans lacking nicotinamide adenine dinucleotide-specific glutamate dehydrogenase (nad-gdh) have been isolated, and their mutations (gdhb1 through gdhb10) have been shown to lie in the gdhb gene. in addition, a temperature-sensitive gdhb mutant (gdhb11) has been isolated. a revertant (designated r-5) of the mutant gdhb1 bears an additional lesion in the gdhb gene and has altered nad-gdh activity with altered km values for ammonia or ammonium ions and for alpha-ketogl ...1976173707
sexual differentiation in aspergillus nidulans: the requirement for manganese and the correlation between phosphoglucomutase and the synthesis of reserve material.aspergillus nidulans was completely devoid of fruit bodies when grown on manganese deficient cultures. this result was shown earlier to be due to a lack of alpha-1,3 glucan in the cell wall. several enzymes of carbon and nitrogen metabolism were investigated in an attempt to explain the absence of this reserve material. synthesis of glucose-6-phosphate dehydrogenase, phosphoglucoisomerase and aldolase, were not strongly affected by manganese deficiency. however, phosphoglucomutase showed only 60 ...1975173248
a mutant of aspergillus nidulans defective in nad-linked glutamate dehydrogenase.a mutation leading to partial loss of nad-linked ("catabolic') glutamate dehydrogenase does not affect the regulation of ammonium-repressible activities in aspergillus nidulans. this mutation has been used to show that nad-linked glutamate dehydrogenase does not normally participate in ammonium assimilation. a mutation leading to loss of nadp-linked ("anabolic') glutamate dehydrogenase has been used to show that nadp-linked glutamate dehydrogenase is not normally involved in glutamate catabolism ...1975172777
kinetics and morphology of glucose-limited cultures of moulds grown in a chemostat and on solid media.the affinity (ks value) of geotrichum candidum for glucose determined from chemostate cultures was ca. 1 mg/1. ks values for glucose was also estimated from the radial growth rates of colonies of g. candidum and neurospora crassa grown on media solidified with agar or silica gel. an assessment is made of the use of colony radial growth rate to determine substrate affinities. the length of apical and intercalary hyphal comparte ments, internode length and the diameter of leading hyphaat the margi ...1975168829
carbon catabolite repression in aspergillos nidulans. 1975168071
regulation of mannitol-1-phosphate dehydrogenase in aspergillus nidulans. 1975163669
mechanism of action of monoketo-organomycin, cystaurimycin and their performic acid-oxidized modifications. ii. inhibition of mitochondrial atpase activity in aspergillus nidulans and identification of the performic acid-oxidized modifications-binding protein. 1979159889
short-term tests for carcinogens and mutagens. 1979159413
adenosine triphosphatase of aspergillus nidulans: existence of isoenzymes of ca2+-atpase.ca2+-atpase activity increased five- to six fold when the cells were subjected to growth at 37 degrees c in protein hydrolysate-supplemented media as compared to that of the cells grown in minimal media. one major isoenzyme and one minor isoenzyme were present in minimal-medium-grown cells while two major isoenzymes were present in the cells grown in protein-supplemented media. when the cells were subjected to heat stress (43 degrees c), they exhibited significantly decreased activity as compare ...1979158522
mitochondrial atpase complex of aspergillus nidulans and the dicyclohexylcarbodiimide-binding protein.the dicyclohexylcarbodiimide-binding protein of aspergillus nidulans has been identified as the smallest subunit of the mitochondrial atpase complex, and has a molecular weight of approximately 8000. it is extractable from whole mitochondria and from the purified enzyme in neutral chloroform/methanol, contains 30% polar amino acids, and the n-terminal amino acid has been identified as tyrosine. using a double-labelling technique in the absence and presence of cycloheximide, followed by immunopre ...1979157278
nitrate assimilation in fungi. 1978157059
nuclear-extranuclear interactions affecting oligomycin resistance in aspergillus nidulans.the extranuclear mitochondrial oligomycin-resistant mutation of aspergillus nidulans, (olia1), was transferred asexually into four nuclear oligomycin-resistant strains of different phenotypes. in all four cases, the possession of the nuclear plus extranuclear mutation led to an increase in the in vivo level of oligomycin resistance. in two cases, the altered cytochrome spectrum and impaired growth ability determined by (olia1) were suppressed by the nuclear mutations. in the third case, the in v ...1977144864
identification of an intramitochondrially synthesized proteolipid associated with the mitochondrial atpase complex as the product of a mitochondrial gene determining oligomycin resistance in aspergillus nidulans. 1977142636
adenosine triphosphatase of aspergillus nidulans: effect of growth temperature. 1976140148
atmospheric mutagens. i. sulfur oxides and nitrogen oxides. 1976134284
compartmentation and regulation of fungal metabolism: genetic approaches. 1975129029
pyrimidine biosynthesis in aspergillus nidulans. isolation and characterisation of mutants resistant to fluoropyrimidines.mutants resistant to 5-fluorouracil, 5-fluorouridine and 5-fluorodeoxyuridine have been selected in aspergillus nidulans. growth tests combined with genetic analysis showed that mutations conferring resistance to fluoropyrimidines could occur in at least seven genes. three of these fule, fulf and fura were concerned with either the uptake of pyrimidines or their conversion to uridine monophosphate. the other four genes did not affect these functions. mutations in fula probably confer resistance ...1975127929
in vitro restoration of nitrate reductase: investigation of aspergillus nidulans and neurospora crassa nitrate reductase mutants.extracts of aspergillus nidulans wild type (bi-1) and the nitrate reductase mutant niad-17 were active in the in vitro restoration of nadph-dependent nitrate reductase when mixed with extracts of neurospora crassa, nit-1. among the a. nidulans cnx nitrate reductase mutants tested, only the molybdenum repair mutant, cnxe-14 grown in the presence of 10-minus 3 m na2 moo4 was active in the restoration assay. aspergillus extracts contained an inhibitor(s) which was measured by the decrease in nadph- ...1975123779
role of aspergillus and candida species in allergic bronchopulmonary mycoses. a comparative study.allergic bronchopulmonary aspergillosis (abpa) and allergic bronchopulmonary candidiasis (abpc) has been diagnosed in 20 and 13 cases respectively with one case in common, on the basis of laboratory and clinical findings. most of the abpa cases (60%) diagnosed had an early onset of respiratory symptoms, i.e. below the age of 30 years, while most of abpc cases (69%) had a late onset of respiratory symptoms, i.e. after the age of 30 years. the precipitin bands in abpa and abpc were r-type and h-ty ...1979118528
[fungi in pulmonary pathology].the author presents the most frequently observed pulmonary infections due to some opportunistic fungi (yeast, aspergillus, mucor). he describes the criterial for a correct diagnosis of pulmonary mycosis.1979114435
[effect of lysozyme, ethylenediaminetetraacetate, magnesium and mannittol on spheroplast formation in anacystis nidulans].three periods (the lag period and periods of intensive and decelerated spheroplast formation) can be detected in the action of lysozyme on the cells of anacystis nidulans; this seems to be due to peculiarities in the cell wall structure of the cyanobacterium and heterogeneity of the culture. edta at concentrations of 0.175--0.7 mm has effect on the duration of the lag period but increases the rate of spheroplast formation during the subsequent periods. however, the action of edta in complex with ...1979113653
photoreactivation of ultraviolet irradiated blue-green alga: anacystis nidulans and cyanophage as-1.ultraviolet (uv) inactivation and photoreactivation of anacystis nidulans and cyanophage as-1 was studied at different wavelengths. uv inactivation of free phage particles and one and two hour host-phage complexes (intracellular phages) were exponential. uv resistance of plaque forming units was attained at the latter phase of latent period. black, blue and white lights were able to photoreactivate the uv irradiated a. nidulans whereas green, yellow and red lights were not. however, incubation o ...1979110290
cell cycle mutants. 1978106766
effect of growth temperature on lipid and fatty acid compositions in the blue-green algae, anabaena variabilis and anacystis nidulans.the lipid composition was affected by growth temperature in anacystis nidulans, but was not in anabaena variabilis. a. variabilis contained fatty acids of 18 and 16 carbon atoms, which were localized at 1- and 2-positions, respectively, of the glycerol moiety of lipids. desaturation of c18 acids was affected by the growth temperature. a. nidulans contained fatty acids of 14, 16 and 18 carbon atoms. monounsaturated and saturated acids were esterified mainly to 1- and 2-position, respectively. des ...1979104734
phycobiliprotein synthesis in protoplasts of the unicellular cyanophyte, anacystis nidulans.stable and metabolically active protoplasts were prepared from the unicellular cyanophyte, anacystis nidulans, by enzymatic digestion of the cell wall with 0.1% lysozyme. the yield of protoplasts from intact algal cells was approx. 50%. incorporation of l-[u-14c]leucine into cold trichloroacetic acid-insoluble material from protoplasts preparations was linear for 1.5 h and continued for an additional 2.5 h. incorporation of radiolabeled leucine into hot trichloroacetic acid-insoluble material fr ...197896857
characterization of antigens from aspergillus fumigatus. iii. comparison of antigenic relationships of clinically important aspergilli.antigenic relationships between strains of aspergillus fumigatus, aspergillus fumigatus var. elipticus, aspergillus phialiseptus, aspergillus flavus, and aspergillus niger were analyzed by fused rocket immunoelectrophoresis and by skin tests. seventy-three to 89 % of the numbers of antigens detected between strains and species of the a. fumigatus series were shared. the degree of sharing between antigens of a. flavus, a. fumigatus series, and a. niger was much lower and ranged from 19 to 35 %. i ...197992906
catabolite repression in aspergillus nidulans; the role of glutamine synthetase.a mutant (nit8) with a lowered activity of glutamine synthetase (gs) was isolated in aspergillus nidulans. the levels of gs and of an arginine catabolic enzyme, ornithine transaminase (ota) were assayed under a variety of growth conditions leading to repression, depression and induction of ota in the wild type, nit8 and several regulatory mutants. the results obtained appear to exclude the possibility of involvement of gs in the regulation of arginine catabolism in a. nidulans.197886277
effect of the area gene on regulation of arginine catabolism in aspergillus nidulans.the area gene which is known to be involved in ammonium repression in aspergillus nidulans was found to participate in regulation of arginine catabolism. mutations in this gene are hypostatic to mutations in arca, sudpro and suepro genes which are responsible for regulation of synthesis of arginine catabolic enzymes.197662492
studies on the role of the area gene in the regulation of nitrogen catabolism in aspergillus nidulans.mutants of apergillus nidulans with lesions in a gene, area (formerly called amdt), have been isolated by a variety of different selection methods. the area mutants show a range of pleiotropic growth responses to a number of compounds as sole nitrogen sources, but are normal in utilization of carbon sources. the levels of two amidase enzymes as well as urease have been investigated in the mutants and have been shown to be affected by this gene. most of the area mutants have much lower amidase-sp ...197552352
phenotypic changes in the chemistry of aspergillus nidulans: influence of culture conditions on mycelial composition.a quantitative study was made of macromolecular (nucleic acids, protein), carbohydrate and mineral (magnesium, potassium and phosphorus) components of aspergillus nidulans in glucose limited chemostat cultures, under varying conditions of dilution rate, temperature, ph and nacl concentration. the overall mineral content showed greatest variation in response to changes in culture salinity, which also affected the mycelial carbohydrate content. concomitant and opposite changes in the content of ca ...197944181
factors affecting protein synthesis during biotin deficiency in aspergillus nidulans.biotin deficiency in aspergillus nidulans resulted in a 70% increase of the protein content and increased levels of free and bound aspartate, glutamate, serine, leucine and methionine. likewise, the activities of nadp+ glutamate dehydrogenase, nad+ gluatmate dehydrogenase, aspartate aminotransferase and alanine aminotransferase were significantly increased. the total rna content increased while the dna content was unaffected. the rrna/trna ratio remained higher in biotin-deficient cells. supplem ...197943277
further characterization of the reduced nicotinamide adenine dinucleotide phosphate: nitrate oxidoreductase in aspergillus nidulans.the reduced nicotinamide adenine dinucleotide phosphate (nadph):nitrate oxidoreductase (ec from aspergillus nidulans wild-type bi-1 was purified by means of salt fractionation, gel filtration, affinity chromatography, and polyacrylamide gel electrophoresis. enzyme which was adsorbed on cibacron blue agarose could be eluted with 2 mm nadph or 2 mm oxidized nadp (nadp(+)), the former being about three times more effective than the latter. about half the total nadph:nitrate reductase activ ...197933144
increased and decreased sensitivity to carbon catabolite repression of enzymes of acetate metabolism in mutants of aspergillus nidulans.the crea204, creb15 and crec27 mutations have been shown to cause carbon catabolite derepression of acetly coa synthase and isocitrate lyase in aspergillus nidulans. a recessive mutation, cre-34, which is linked to the crec gene, results in these enzymes being more sensitive than cre or wildtype strains to catabolite repression. the acetamidase levels of strains containing cre mutations have been investigated and provide support for the hypothesis that an acetate metabolite, rather than acetamid ...197723491
mutagenic and recombinogenic action of pesticides in aspergillus nidulans.thirteen pesticides, aminotriazole, benomyl, captafol, captan, dalapon-na, dichlorvos, dinobuton, dodine, ioxynil, mecoprop, neburon, picloram and tordon were tested for ability to induce (1) point mutations to 8-azaguanine resistance, (2) mitotic crossing-over, and (3) mitotic non-disjunction and haploidization in aspergillus nidulans. tests were performed at three different phs, i.e. 4.5, 7, 8.2. three of the pesticides, captan , captafol and dichlorvos induced point mutations; dichlorvos also ...197722812
induction of the acetamidase of aspergillus nidulans by acetate metabolism.growth tests and enzyme determinations strongly suggest that the acetamidase of aspergillus nidulans is induced by a product of acetate metabolism rather than the substrate, acetamide. the cis-dominant mutation, amdi9, which is closely linked to amds, the structural gene for the acetamidase, results in greatly increased sensitivity to induction by acetate metabolism. propionate, l-threonine, and ethanol also result in acetamidase induction. mutations in the faca, facb, and facc genes, which lead ...197719418
in vivo and in vitro studies of nitrate reductase regulation in asperillus nidulans.induced wildtype cells of a. nidulans rapidly lost nadph--linked nitrate reductase activity when subjected to carbon and or nitrogen starvation. a constitutive mutant at the regulatory gene for nitrate reductase, nir ac 1, rapidly lost nitrate reductase activity upon carbon starvation. this loss of activity is thought to be due to a decrease in the nadph concentration in the cells. cell free extracts from wildtype cells grown in the presence of nitrate, rapidly lost their nitrate reductase activ ...197717826
differential binding of methyl benzimidazol-2-yl carbamate to fungal tubulin as a mechanism of resistance to this antimitotic agent in mutant strains of aspergillus nidulans.the antimitotic compound methyl benzimidazol-2-yl carbamate (mbc) formed a complex in vitro with a protein present in mycelial extracts of fungi. the binding protein of aspergillus nidulans showed a set of properties which is unique for tubulin. binding occurred rapidly at 4 degrees c and was competitively inhibited by oncodazole and colchicine. other inhibitors of microtubule function such as podophyllotoxin, vinblastine sulfate, melatonin, and griseofulvin did not interfere with binding of mbc ...197712184
chlorate toxicity in aspergillus nidulans. studies of mutants altered in nitrate had previously been held that chlorate is not itself toxic, but is rendered toxic as a result of nitrate reductase-catalysed conversion to chlorite. this however cannot be the explanation of chlorate toxicity in aspergillus nidulans, even though nitrate reductase is known to have chlorate reductase activity. among other evidence against the classical theory for the mechanism of chlorate toxicity, is the finding that not all mutants lacking nitrate reductase are clorate resistant. both chlorat ...19768697
assimilation of ammonia and growth of biotin deficient aspergillus nidulans.biotin deficiency in aspergillus nidulans has been found to increase the uptake of ammonium ions, associated with a marked increase in the activity of nadp-linked glutamate dehydrogenase, which is found to be the major route of ammonia assimilation in this culture. the results obtained are discussed with respect to the growth of aspergillus nidulans during biotin deficiency.19765283
analysis of acetate non-utilizing (acu) mutants in aspergillus nidulans.genetic analysis of 119 acetate non-utilizing (acu) mutants in aspergillus nidulans revealed ten new loci affecting acetate metabolism in addition to the three previously recognized on the basis of resistance to fluoroacetate and acetate non-utilization. the enzyme lesions associated with mutations at seven of the acu loci are described. these are: faca (= acua), acetyl-coa synthase; acud, isocitrate lyase; acue, malate synthase; acuf, phosphoenolpyruvate carboxykinase; acug, fructose 1,6-diphos ...19763622
basic and neutral amino acid transport in aspergillus nidulans.arginine and methionine transport by aspergillus nidulans mycelium was investigated. a single uptake system is responsible for the transport of arginine, lysine and ornithine. transport is energy-dependent and specific for these basic amino acids. the km value for arginine is 1 x 10(-5) m, and vmax is 2-8 nmol/mg dry wt/min; km for lysine is 8 x 10(-6) m; kt for lysine as inhibitor of arginine uptake is 12 mum, and ki for ornithine is mm. on minimal medium, methionine is transported with a km of ...19761466
a modified minimal medium for aspergillus nidulans. 1975328
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