Publications

site-directed mutagenesis of the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase from anacystis nidulans.	using oligonucleotide-directed mutagenesis of the gene encoding the small subunit (rbcs) from anacystis nidulans mutant enzymes have been generated with either trp-54 of the small subunit replaced by a phe residue, or with trp-57 replaced by a phe residue, whereas both trp-54 and trp-57 have been replaced by phe residues in a double mutant. trp-54 and trp-57 are conserved in all amino acid sequences or the small subunit (s) that are known at present. the wild-type and mutant forms of rubisco hav ...	1987	3030746
cloning and characterization of the alda gene of aspergillus nidulans.	we have cloned and sequenced the alda (encoding aldehyde dehydrogenase) gene of aspergillus nidulans. the gene contains two introns which are similar in size and structure to other fungal introns. the amino acid sequence of aldehyde dehydrogenase (497 residues) shows a significant level of homology with analogous sequences in other organisms. comparison of the primary structure of the active sites of the mammalian cytosolic and mitochondrial enzymes shows that the aspergillus enzyme closely rese ...	1987	3036652
the involvement of glutamine synthetase/glutamate synthase in ammonia assimilation by aspergillus nidulans.	wild-type aspergillus nidulans grew equally well on nh4cl, kno3 or glutamine as the only nitrogen source. nadp+-dependent glutamate dehydrogenase (ec 1.4.1.4) and glutamine synthetase (gs; ec 6.3.1.2) activities varied with the type and concentration of nitrogen source supplied. glutamate synthase (gogat) activity (ec 1.4.7.1) was detected but it was almost unaffected by the type and concentration of nitrogen source supplied. ion exchange chromatography showed that the gogat activity was due to ...	1987	2888838
the nucleotide sequence of the amds gene of aspergillus nidulans and the molecular characterization of 5' mutations.	the structure of the amds gene of aspergillus nidulans has been determined by nucleotide sequence analysis. the coding sequence is interrupted by three small introns with splicing signals consistent with other fungal genes. possible tata and caat elements are found upstream of the start point of transcription. sequence changes in mutations in the 5' region of the gene have been determined. two deletions including the start point of transcription abolish detectable transcripts. a series of mutati ...	1987	3036667
cloning of the ribob locus of aspergillus nidulans.	we have complemented the ribob2 mutation of aspergillus nidulans by transformation with a plasmid library of wild-type (wt) sequences. we have isolated, by marker rescue from a ribob+ transformant, a plasmid that complements ribob2 efficiently. from this plasmid we have subcloned an a. nidulans sequence that complements ribob2 efficiently and that integrates by homologous recombination at a site closely linked to the ribob locus. we conclude that this sequence contains the wt ribob+ allele.	1987	3038695
selectable genes for transformation of the fungal plant pathogen glomerella cingulata f. sp. phaseoli (colletotrichum lindemuthianum).	glomerella cingulata f. sp. phaseoli (gcp) was transformed using either of two selectable markers: the amds + gene of aspergillus nidulans, which encodes acetamidase and permits growth on acetamide as the sole nitrogen source and the hygbr gene of escherichia coli which encodes hygromycin b (hy) phosphotransferase and permits growth in the presence of the antibiotic hy. the amds+ gene functioned in gcp under control of a. nidulans regulatory signals and hygbr was expressed after fusion to a prom ...	1987	3038698
high level of complexity of small nuclear rnas in fungi and plants.	the complexity of the trimethylguanosine-capped, small nuclear rna (snrna) populations in a number of organisms has been examined using immunoprecipitation and two-dimensional gels. from the fungi aspergillus nidulans and schizosaccharomyces pombe, over 30 major snrnas can be resolved. the most abundant of these correspond to the putative analogues of vertebrate u1, u2, u4 and u5, which have been reported to be precipitated by anti-sm antibodies, but other snrnas are little less abundant than th ...	1987	2958638
the role of the n-terminus of the large subunit of ribulose-bisphosphate carboxylase investigated by construction and expression of chimaeric genes.	the genes for the large and small subunits of ribulose bisphosphate carboxylase/oxygenase (rubisco) from anacystis nidulans have been expressed in escherichia coli under the control of the lac promoter to produce active enzyme. the enzyme can be purified from the cells to yield up to 200 mg rubisco/l cultured bacteria, and is indistinguishable from the enzyme extracted from a. nidulans. in order to investigate the role of the n-terminus of the large subunit in catalysis, chimaeric genes were con ...	1987	3121325
nitrate starvation induces homeoviscous regulation of lipids in the cell envelope of the blue-green alga, anacystis nidulans.	replacement of the normal culture liquid to a nitrate-free medium resulted in an immediate drop in the ratio of protein to lipid in isolated cell envelopes of anacystis nidulans cells. the relative fluidity of the envelope membranes or liposomes, made from the extracted lipids of the envelope, was estimated by measuring the steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene. a thermotrophic phase transition of lipids within the cytoplasmic membrane of intact cells was also r ...	1987	3109903
uptake and expression of bacterial and cyanobacterial genes by isolated cucumber etioplasts.	the uptake and expression by plastids isolated from dark-grown cucumber cotyledons (etioplasts) of two puc derivatives, pcs75 and puc9-cm, respectively carrying genes for the large small subunits of ribulose bisphosphate carboxylase/oxygenase of anacystis nidulans or chloramphenicol acetyltransferase, is reported. untreated etioplasts take up only 3% as much dna as that taken up by edta-washed etioplasts after 2 hr of incubation with nick-translated [32p]-pcs75. the presence or absence of light ...	1987	3114748
nucleotide sequence of the gene from the cyanobacterium anacystis nidulans r2 encoding the mn-stabilizing protein involved in photosystem ii water oxidation.	the gene for the mn-stabilizing protein (msp; the so-called extrinsic 33-kda protein) that is involved in photosystem ii water oxidation was cloned and sequenced from the genome of the cyanobacterium anacystis nidulans r2. the gene (here designated woxa) was shown to be present in a single copy. the deduced amino acid sequence indicated that the translation product consisted of 277 amino acid residues with a mr of 29,306. the comparison of the sequence with that of mature msp from spinach chloro ...	1987	3120187
evaluation of commercial serologic test reagents for immunoidentification of medically important aspergilli.	we evaluated commercial serodiagnostic test reagents from greer laboratories (gl), lenoir, nc; immuno-mycologics, inc. (imi), norman, ok; and scott laboratories (sl), fiskville, ri; for their ability to detect aspergillus spp. exoantigens and group them in their proper series. we detected 87 culture extracts from coded cultures of aspergillus groups and heterologous fungi against anti-a. fumigatus, a. flavus, a. nidulans, a. niger, and a. terreus sera in the presence of their corresponding antig ...	1987	3126020
an endogenous inducer of sexual development in aspergillus nidulans.	during development of the homothallic ascomycete aspergillus nidulans, asexual sporulation is followed by sexual sporulation. we report here the detection of a solvent-extractable activity which inhibits asexual sporulation and stimulates premature sexual sporulation. this activity, called precocious sexual inducer (psi), is overproduced by certain mutants that are blocked in both modes of sporulation. using partially purified preparations of psi, biological response could be elicited with as li ...	1987	3309182
a versatile transformation system for the cellulolytic filamentous fungus trichoderma reesei.	an efficient transformation system for the cellulolytic filamentous fungus trichoderma reesei has been developed. transformation was obtained with plasmid carrying the dominant selectable marker amds or the argb gene of aspergillus nidulans, which was found to complement the respective argb mutation of t. reesei. the transformation frequency can be up to 600 transformants per microgram of transforming dna. the efficiency of co-transformation with unselected dna was high (approx. 80%). the transf ...	1987	3127274
saprophytic fungi isolated from animal and bird pens in egypt.	forty-four samples collected from animal and bird pens were screened for their content of saprophytic fungi by using the dilution plate method. 76 species in addition to one variety of aspergillus flavus belonging to 33 genera were recovered on three types of media: 20 genera and 49 species on littman-oxgall agar, 19 genera and 41 species on cellulose- and 19 genera and 43 species on glucose-czapek's agar. the most frequent genera were aspergillus (21 species), scopulariopsis (4 species) and pen ...	1987	3130473
transformation of penicillium chrysogenum using the aspergillus nidulans amds gene as a dominant selective marker.	the aspergillus nidulans acetamidase gene (amds) has been used to transform penicillium chrysogenum at low frequency. several transformants were tested and shown to be mitotically stable. southern blot analysis indicated that transforming dna had integrated into the chromosomal dna, possibly at multiple sites.	1987	3131026
phycocyanin alpha-subunit gene of anacystis nidulans r2: cloning, nucleotide sequencing and expression in escherichia coli.	the cloning and nucleotide sequence determination of the anacystis nidulans r2 phycocyanin (pc) alpha-subunit gene are described. a 3.0-kb psti fragment of anacystis nidulans r2 genomic dna cloned in plasmid puc8 was found to hybridize with a heptadecameric oligodeoxynucleotide probe. sequencing using synthetic primers revealed the presence of the pc alpha-subunit gene and the 3' proximal end of the beta-subunit gene. the alpha-gene is separated from the upstream beta-gene by a spacer length of ...	1987	3036657
antisuppressor mutations in aspergillus nidulans: cold-resistant revertants of suppressor suac109.		1987	3305170
emericella nidulans in a maxillary sinus fungal mass.	sexual reproductive stages of fungi are very rarely found within mammalian tissues. we report here coexistence of cleistothecia associated with emericella nidulans and its conidial state, aspergillus nidulans, in a fungal mass which developed in a maxillary sinus.	1987	3323452
monoclonal antibody probes for the niad specified subunit in the nadph-nitrate reductase from aspergillus nidulans.	in aspergillus nidulans, the nitrate assimilatory pathway is regulated by a variety of agents, one being the autogenous enzyme nitrate reductase. a major subunit of the enzyme which is specified by the niad structural gene and is implicated in autogenous control exhibits both nitrate inducible diaphorase activity and ammonium repression. the former was used to test the extent to which alterations in the niad specified protomer might affect its formation in selected niad point and deletion mutant ...	1987	3323853
chemical and biological characterization of hazardous industrial waste. ii. eukaryotic bioassay of a wood-preserving bottom sediment.	the eukaryotic haploid and diploid forms of aspergillus nidulans were used to detect gene mutations and various types of chromosome damage, respectively, in the acid, base and neutral fractions of a wood-preserving bottom sediment. the corresponding response to prokaryotic mutagenicity assays and major chemical constituents of the 3 waste fractions were described by donnelly et al. (1987). the haploid methionine system detected genotoxic compounds in all 3 primary waste fractions without metabol ...	1987	3306353
demonstration of an altered phenylalanyl-trna synthetase in an analogue-resistant mutant of aspergillus nidulans.	we have isolated and characterized a new class of p-fluorophenylalanine (fpa)-resistant mutant in aspergillus nidulans using a phena strain as the wild type, by optimizing the conditions of growth. all four spontaneous mutants selected on a medium containing fpa were found to be recessive to their wild-type alleles in heterozygous diploids. complementation analyses and linkage data showed that they were allelic and mapped at a single locus (fpau) in the faca-ribod interval on the right arm of li ...	1987	3312953
cotransformation of aspergillus nidulans: a tool for replacing fungal genes.	when a non-selected dna sequence was added during the transformation of amds320 deletion strains of aspergillus nidulans with a vector containing the wild-type amds gene the amds+ transformants were cotransformed at a high frequency. cotransformation of an amds320, trpc801 double mutant strain showed that both the molar ratio of the two vectors and the concentration of the cotransforming vector affected the cotransformation frequency. the maximum frequency obtained was defined by the gene chosen ...	1987	3312958
isolation and characterization of the positively acting regulatory gene quta from aspergillus nidulans.	the positively acting regulator gene quta from aspergillus nidulans has been identified and located within a cluster of quinic acid utilisation (qut) genes isolated within a recombinant phage lambda (lambda q1). the dna sequence of the quta gene reveals a single uninterrupted reading frame coding for a protein of mw 90.416 kd. the quta protein sequence has a protein motif in the form of a putative "dna finger" that shows strong homology to other such motifs in the gal4, ppr1, argrii, lac9 and qa ...	1987	3313276
13c-nmr analysis of aspergillus mutants disturbed in pyruvate metabolism.	the metabolic consequences of two defects in pyruvate metabolism of the hyphal fungus aspergillus nidulans have been investigated by natural abundance 13c-nmr spectroscopy. a pyruvate dehydrogenase complex (pdh) mutant, grown on acetate, accumulates alanine upon starvation which is derived from mannitol reserves. the l-alanine level increases further upon incubation with the non-permissive substrate d-glucose. l-glutamate is absent from these spectra as it is required both for the transamination ...	1987	3315006
[modern electron microscopy at cellular and macromolecular levels. strategies for preparation, imaging and image interpretation].	conventional electron microscopy has significantly contributed to the understanding of structure-function relationships in living systems on cellular and macromolecular levels. new approaches and strategies will provide further insight into the organization of life. these new developments include cryopreparation and imaging techniques, x-ray microanalysis on frozen samples, electron energy loss spectroscopy, electron spectroscopic imaging, electron microscopic immunocytochemistry, preparation an ...	1987	3317069
cloning and characterization of the isopenicillin n synthetase gene mediating the formation of the beta-lactam ring in aspergillus nidulans.	genomic clones containing an aspergillus nidulans isopenicillin n synthetase (ipns) gene have been identified by heterologous hybridization with a cephalosporium acremonium dna probe. the open reading frame encodes a 331 amino acid polypeptide with extensive homology with the genes of other beta-lactam-producing fungi. the gene product has been overexpressed in escherichia coli and shown to have activity of ipns. this represents the first evidence at the molecular level that the biosynthesis of ...	1987	3319778
development of a homologous transformation system for aspergillus niger based on the pyrg gene.	the development of a homologous transformation system for aspergillus niger is described. the system is based on the use of an orotidine-5'-phosphate decarboxylase deficient mutant (pyrg) and a vector, pab4-1, which contains the functional a. niger pyrg gene as a selection marker. transformation of the a. niger pyrg mutant with pab4-1 resulted in the appearance of stable pyr+ transformants at a frequency of 40 transformants per microgram of dna. in 90% of these transformants integration had occu ...	1987	3472035
regulation of the mrna levels of nima, a gene required for the g2-m transition in aspergillus nidulans.	the temperature-sensitive cell cycle mutation nima5 causes nuclei of aspergillus nidulans to be blocked in late g2 at restrictive temperature. under these conditions the spindle pole body divides but does not separate and the mitotic index drops to zero. if nima5 is blocked for more than one doubling time and then shifted from restrictive to permissive temperature, nuclei immediately enter mitosis, the mitotic spindle forms, and the chromosomes condense (oakley, b. r., and n. r. morris, 1983, j. ...	1987	3294854
comparison of the cis-acting control regions of two coordinately controlled genes involved in ethanol utilization in aspergillus nidulans.	the alca and alda genes of aspergillus nidulans are regulated in exactly the same manner, being subject to positive control by the product of the alcr gene. we report the complete nucleotide sequence of the alca gene and its 5' non-coding region, preliminary localization of the region involved in the regulation of alca expression, and a detailed comparison of this region to the 5' non-coding region of alda (pickett et al., 1987). the 5' flanking regions of the genes contain six similar sequence ...	1987	3297923
conditionally lethal tuba alpha-tubulin mutations in aspergillus nidulans.	we have mapped 17 extragenic suppressors of bena33, a heat-sensitive beta-tubulin mutation of aspergillus nidulans, to the tuba alpha tubulin locus. fifteen of these tuba mutations cause cold sensitivity in a genetic background with bena33 and appear to cause lethality in a background with the wild-type bena allele. we examined the microtubule-mediated processes, nuclear division and nuclear migration, in seven different cold-sensitive double mutants, each carrying bena33 and a different cold-se ...	1987	3302605
proteolysis by toxigenic aspergillus nidulans from nigerian palm produce.	the submerged cultures of aspergillus nidulans had optimal growth and protease production at 37 degrees c and within 6 days of incubation. a rapid drop in ph of the growth medium from 6.9 to 4.8 and a subsequent gradual rise was recorded with the period of incubation. the acid-protease produced was purified by a combination of ethanolic precipitation, ultrafiltration and fractionation on deae-cellulose and sephadex g-200. a single peak showing protease activity was subsequently obtained with a 1 ...	1987	3302717
the unique histone h2a gene of aspergillus nidulans contains three introns.	the histone h2a gene of the filamentous fungus aspergillus nidulans has been cloned and sequenced. there is a single h2a gene in the genome of a. nidulans, and it contains three introns. the introns are 51 nucleotides (nt), 56 nt and 50 nt in length and split codons for amino acids (aa) 18, 48 and 116 of the predicted protein. the transcriptional start and termination points have been determined using an s1 nuclease protection assay. the predicted protein is 132 aa residues in length and surpris ...	1987	3319784
microheterogeneity in aspergillus nidulans 5s rrna genes.	we have determined the sequence of 4 aspergillus nidulans 5s rrna genes and compared it with 4 previously established sequences. no extensive homologies are found in 5' flanking sequences, but in the 3' flanks of two genes and two pseudogenes similar sequences are observed. in the coding sequences differences occur in 7 positions. two 5s rrna genes which are found in one plasmid 1.1 kb apart are located in opposite orientations.	1987	3329974
suppressors suac109 and suaa101 of aspergillus nidulans alter the ribosomal phenotype in vitro.	a new homologous, cell-free system for protein synthesis has been devised for use with ribosomes and elongation factors from aspergillus nidulans. ribosome preparations from strains with either the suaa101 or suac109 mutations have a higher misreading ratio (non-cognate:cognate amino acid incorporation) in the presence of hygromycin than controls. they can be classed as fidelity mutants. these results also prove that the mutations must be in genes coding for ribosomal proteins or enzymes which m ...	1987	3331121
cloning, mapping and molecular analysis of the pyrg (orotidine-5'-phosphate decarboxylase) gene of aspergillus nidulans.	we have modified the transformation procedures of ballance et al. [biochem. biophys. res. commun. 112 (1983) 284-289] to give increased rates of transformation in aspergillus nidulans. with the modified procedures we have been able to complement pyrg89, a mutation in the orotidine-5'-phosphate decarboxylase gene of a. nidulans, by transformation with a library of wild-type (wt) sequences in pbr329. we have recovered, by marker rescue from one such transformant, a plasmid (pjr15) that carries an ...	1987	3328733
fixing on an enigma.		1987	3153172
on the mechanism of mitotic segregation induction in aspergillus nidulans by benzene hydroxy metabolites.	the principal hydroxy-metabolites of benzene--hydroquinone, catechol and phenol--were assayed in tests for mitotic segregation induction in aspergillus nidulans diploid strain 19. hydroquinone was the most effective chemical, increasing the frequency of mitotic segregants up to 10-fold at 1-3 mm. catechol was similarly active at 10-20 mm and phenol was weakly positive at 15 mm. genetic characterization of induced abnormal segregating colonies by replating and complementary assays with haploid st ...	1987	3325750
the mitochondrial genome of the fission yeast, schizosaccharomyces pombe. sequence of the large-subunit ribosomal rna gene, comparison of potential secondary structure in fungal mitochondrial large-subunit rrnas and evolutionary considerations.	the dna sequence of the mitochondrial large subunit (lsu) rrna gene of schizosaccharomyces pombe has been determined. in the direction of transcription, this gene is located between the gene coding for subunit ii of cytochrome oxidase and a cluster of three trna genes. both the 5' and 3' ends of the lsu rrna have been mapped precisely: whereas the 5' end can be assigned unambiguously to a single nucleotide position, multiple 3' ends occur within a run of eight u residues. based on these results, ...	1987	2446871
transformation of aspergillus nidulans by the argb gene.	aspergillus nidulans argb mutant was transformed with the plasmid dna containing the argb gene. analysis of transformants revealed that transformation was due to integration of either argb gene alone or the whole plasmid dna into the a. nidulans genome. in 5 out of 23 transformants studied, integration took place in the locus different than the original argb locus. the amplification of integrated sequences was often observed. integrated dna was found to be mitotically stable, while the meiotic s ...	1987	2442970
the complex arom locus of aspergillus nidulans. evidence for multiple gene fusions and convergent evolution.	the physical positions of the dna sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-d-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the a. nidulans arom polypeptide have been determined. subfragments of the arom locus encoding epsp synthase and 3-dehydroquinase have been expressed in appropriate e. coli aro mutants. the dna sequence of the a. nidulans arom locus has been shown to have homology with the cor ...	1987	2836080
transformation of aspergillus niger using the homologous orotidine-5'-phosphate-decarboxylase gene.	a homologous transformation system for the filamentous fungus aspergillus niger has been developed, based on the orotidine-5'-phosphate-decarboxylase gene. a. niger pyr- mutants have been selected from 5-fluoro-orotic acid resistant mutants. these mutants were found to comprise two complementation groups, pyra and pyrb. the a. niger omp-decarboxylase gene was isolated from a gene library by heterologous hybridization with the neurospora crassa pyr4 gene. the cloned gene is capable to transform a ...	1987	2836081
fungal small nuclear ribonucleoproteins share properties with plant and vertebrate u-snrnps.	snrnas with properties closely related to those of the major vertebrate u-snrnas are present in the fungi aspergillus nidulans, neurospora crassa and schizosaccharomyces pombe. these rnas possess a tri-methyl guanosine cap structure and a subset cross-hybridizes with human u1 and u2 clones. in the form of snrnps, snrnas from these fungi as well as from saccharomyces cerevisiae and pea plants are immunoprecipitated by human and anti-sm or anti-(u1)rnp autoimmune antibodies. on micro-injection int ...	1987	2953599
complementation of area- regulatory gene mutations of aspergillus nidulans by the heterologous regulatory gene nit-2 of neurospora crassa.	loss-of-function mutations in the regulatory gene area of aspergillus nidulans prevent the utilization of a wide variety of nitrogen sources. the phenotypes of nit-2 mutants of neurospora crassa suggest that this gene may be analogous to the area gene. transformation has been used to introduce a plasmid containing the nit-2 gene into a. nidulans. the nit-2 gene of neurospora complemented mutations in the area gene, restoring the ability to use a variety of nitrogen sources. this indicated that t ...	1987	2954160
identification and isolation of a putative permease gene in the quinic acid utilization (qut) gene cluster of aspergillus nidulans.	mutations in the qutd gene of aspergillus nidulans cause the loss of ability to grow upon quinic acid as sole carbon source in media at normal ph 6.5 and failure to induce three enzyme activities specifically required for metabolism to protochatechuic acid. all 9 qutd mutants recovered are recessive and have been found to be ph sensitive, growing strongly on quinic acid media at ph 3.5 and producing significant induced enzyme activities. these properties are consistent with the hypothesis that t ...	1987	2835177
heat shock phenomena in aspergillus nidulans. ii. combined effect of heat and bleomycin to heat shock protein synthesis, survival rate and induction of mutations.	the combined action of hyperthermia and bleomycin on aspergillus nidulans was studied at three different levels: mycelial protein synthesis, spore viability and induction of mutations. it was found that bleomycin treatment of preincubated mycelia during the heat shock enhances the incorporation of 35s-methionine into heat shock bands. furthermore, simultaneous treatment with hyperthermia (43 degrees c) and bleomycin results in greater cytotoxic activity in spores and in a higher induction rate o ...	1987	2452026
molecular organisation of the quinic acid utilization (qut) gene cluster in aspergillus nidulans.	the functional integrity of the qutb gene (encoding quinate dehydrogenase) has been confirmed by transformation of a qutb mutant strain. the dna sequence of the contiguous genes qutd (quinate permease), qutb and qutg (function unknown) has been determined and analysed, together with that of qute (catabolic 3-dehydroquinase). the qutb sequence shows significant homology with the shikimate dehydrogenase function of the complex arom locus of aspergillus nidulans, and with the qa-3 quinate dehydroge ...	1988	2976880
comparison of the orotidine 5'-monophosphate decarboxylase sequences of eight species.	predicted amino acid sequences of the enzyme orotidine 5'-phosphate decarboxylase (ec 4.1.1.23) from eight different organisms are compared. the comparisons are made on the basis of primary structural differences, primary amino acid sequence, hydropathy profiles, and secondary structure predictions. the organisms compared are mus musculus, aspergillus nidulans, neurospora crassa, kluyveromyces lactis, saccharomyces cerevisiae, schizosaccharomyces pombe, escherichia coli, and salmonella typhimuri ...	1988	2974823
calmodulin-dependent multifunctional protein kinase in aspergillus nidulans.	a ca2+/calmodulin (cam)-dependent multifunctional protein kinase has been isolated from aspergillus nidulans and purified to homogeneity. unlike any cam-dependent multifunctional protein kinase described previously, the native enzyme from aspergillus behaves as a monomer. the calculated molecular weight is 41,200. nadodso4/page reveals a single protein band with an apparent mr of 51,000. two-dimensional isoelectric focusing/nadodso4/page of the purified enzyme showed one major and one minor more ...	1988	2835766
induction of multiple germ tubes in neurospora crassa by antitubulin agents.	the antitubulin fungicide benomyl suppressed the linear growth of neurospora crassa wild type strain st. lawrence 74 at micromolar concentrations. the rate of germination of macroconidia was not affected. macroconidia exposed to 1.7 microm benomyl for 5 h formed multiple germ tubes. when germlings incubated for 4 h were exposed to 1.7 microm benomyl for 3 h, their germ tube stopped growing, swelled and emitted several branches. normal linear growth was restored after removal of the fungicide. li ...	1988	2969337
nucleotide sequence of the aspergillus niger trpc gene: structural relationship with analogous genes of other organisms.	the nucleotide sequence of the aspergillus niger tryptophan c (trpc) gene was determined. northern hybridization and s1-mapping experiments showed the presence of a 2.6 kb trpc poly(a)+ rna with two very short (5 and 6 nucleotides) noncoding 5'-regions. comparison of the predicted amino acid sequence with that of trp gene proteins of pro- and eukaryotic organisms revealed three functional domains (g, c, f) in the a. niger trpc protein which catalyse the glutamine amidotransferase reaction (gat), ...	1988	2836085
repair of alkylation damage in the fungus aspergillus nidulans.	the repair of alkylation damage in aspergillus nidulans was investigated. we have assayed soluble protein fractions for enzymes known to be involved in the repair of this type of damage in dna. the presence of a glycosylase activity that can remove 3-methyladenine from dna was demonstrated, as well as a dna methyltransferase activity that appears to act against o6-methylguanine. in addition to this approach, a series of mutants were isolated which display increased sensitivity to alkylating agen ...	1988	2452348
distinction of cnxh cofactor gene-specified protomers with monoclonal antibodies to aspergillus nitrate reductase.	the nitrate reductase (nadph) (ec 1.6.6.3) from aspergillus nidulans is influenced directly by mutations in the structural gene (niad) for the major subunit of the enzyme and indirectly by mutation in any of several molybdenum cofactor loci (cnx). the cnxe-14 and the cnxh-3 mutants have been noted to contain the enzyme in two distinct forms following induction with nitrate. with the cnxh-3 as a prototype cnxh mutant, 10 other cnxh were found to be devoid of the assembled (dimeric) form of the en ...	1988	2454152
developmental characterization and chromosomal mapping of the 5-azacytidine-sensitive fluf locus of aspergillus nidulans.	in aspergillus nidulans, a fungus that possesses negligible, if any, levels of methylation in its genome, low concentrations of 5-azacytidine (5-ac) convert a high percentage of the cell population to fluffy phenotypic variants through a heritable modification of a single nuclear gene (m. tamame, f. antequera, j. r. villanueva, and t. santos, mol. cell. biol. 3:2287-2297, 1983). this new 5-ac-altered locus, designated here fluf1, was mapped as the closest marker to the centromere that has been i ...	1988	2463470
differential expression of ustilago maydis dna sequences during induction of nitrate reductase enzyme activity.	a differential hybridisation screen of an ustilago maydis genomic dna library was used to identify dna sequences transcribed at higher levels under growth conditions which induce nitrate reductase activity. after two rounds of screening, four different sequences showed a strongly enhanced hybridisation signal with an induced cdna probe relative to a repressed cdna probe. the sequence in plasmid pmh3007 hybridised to a u. maydis rna transcript of a 4.2 kb. this is identical in size to a transcrip ...	1988	3224386
interpretation of uv-survival curves of aspergillus conidiospores.	semi-logarithmic dose-response curves for survival of uv-irradiated conidiospores of a. nidulans have an initial shoulder (at low doses) followed by a decline which becomes linear. to explain the initial shoulder and the resulting extrapolation number (log s intercept of the linear extrapolation line) a general model is presented, which includes multi-target (n) and multi-hit (h) effects and allows for the effect of initial repair and of a compound parameter k, which stands for inherent sensitiv ...	1988	3275884
spindle formation and chromatin condensation in cells blocked at interphase by mutation of a negative cell cycle control gene.	in aspergillus nidulans the temperature-sensitive cell cycle mutation bime7 causes chromosome condensation and pre-anaphase spindle formation to occur at restrictive temperature. by constructing double mutants between bime7 and s phase or g2 phase mutants and blocking dna replication with hydroxyurea, we demonstrate that bime7 can cause chromatin condensation and spindle formation in cells held in s or g2. thus bime7 overrides normal control systems that prevent mitosis from prematurely occurrin ...	1988	3277718
nuclear migration in a nud mutant of aspergillus nidulans is inhibited in the presence of a quantitatively normal population of cytoplasmic microtubules.	nuclear migration was studied in germinating conidia of a temperature-sensitive mutant of the fungus aspergillus nidulans. at the restrictive temperature motility was demonstrably impaired because significantly fewer nuclei migrated into the germ tube relative to a population of similarly sized germlings grown at the permissive temperature. further comparison of these populations showed that the mutant was leaky in that an increasing number of nuclei migrated as the total nuclear content increas ...	1988	3279053
an uvsb mutant of aspergillus nidulans with high variable spontaneous mutation and intergenic mitotic recombination frequencies.	an uv-sensitive mutant has been isolated with a new technique which allows isolation of uv-sensitive and uv-non-mutable mutants in aspergillus nidulans. this mutant is an allele of the known uvsb gene but shows some features not previously described in the alleles so far isolated. its more important characteristics are: (1) frequency of mitotic intergenic recombination is strongly increased in uvs/uvs diploids and it is highly variable in different clones: it varies from a minimum of 40-fold to ...	1988	3283545
mutants of aspergillus nidulans with increased resistance to the alkylating agent, n-methyl-n'-nitro-n-nitrosoguanidine.	the isolation and characterisation of mutants of aspergillus nidulans showing resistance to mnng is described. such isolates were stable through prolonged subculture in the absence of the selective agent, and resistance segregated as an allele of a single gene in meiotic and mitotic analysis. mnng-resistant strains showed an increase in resistance to ems and uv irradiation but no cross-resistance to mms was detected. possible mechanisms of resistance to alkylating agents are discussed.	1988	3283539
a new gene controlling sulphite reductase in aspergillus nidulans.		1988	3284789
similarity of nucleotide sequences at splicing sites.		1988	3287170
brla is necessary and sufficient to direct conidiophore development in aspergillus nidulans.	the brla gene of a. nidulans mediates the developmental switch from the indeterminate, apical growth pattern of vegetative cells to the budding growth pattern of conidiophores. brla encodes a 432 amino acid polypeptide containing two directly repeated motifs resembling the zn(ii) coordination sites first recognized in xenopus tfiiia. misscheduled expression of brla in vegetative cells results in transcriptional activation of developmentally regulated genes, cessation of unidirectional hyphal gro ...	1988	3293800
developmental regulation of a conidiation specific beta-tubulin in aspergillus nidulans.	a beta-tubulin gene previously suggested to participate in conidial development in the filamentous fungus aspergillus nidulans is shown to be developmentally regulated in its expression. a quantitative s1 assay was used to show that the abundance of the tubc messenger rna increases during conidial development relative to the bena messenger rna. morphological analysis of cultures, used to prepare rna for the s1 analysis, demonstrated that the increase in tubc messenger rna was directly correlated ...	1988	3294063
nadph generation in aspergillus nidulans: is the mannitol cycle involved?	a cyclic pathway of nadph generation involving interconversion of mannitol and fructose has been proposed to occur in fungi. in aspergillus nidulans three enzymes of this proposed mannitol cycle (hexokinase, nadp-mannitol dehydrogenase and mannitol-l-phosphate phosphatase) were shown to be localized exclusively in the cytosol. two isoenzymes of the fourth enzyme (mannitol-l-phosphate dehydrogenase) were detected and shown to be localized respectively in the mitochondrion and the cytosol. the mit ...	1988	3141571
regulatory region of the aspergillus nidulans argb gene.	we have constructed a series of deletion plasmids which contain the aspergillus nidulans argb gene for ornithine carbamoyltransferase (otc). these deletions comprise the 5' upstream sequence of the argb gene. the pro- arg- strain of a. nidulans was transformed with the above plasmids. several arg+ transformants of integration types i and ii, obtained using each of the deletion plasmids, were studied, and their ability to de-repress otc level by proline starvation was compared. it was concluded t ...	1988	3066508
isozyme polymorphism of beta-glucosidase in aspergillus nidulans.	electrophoretic analysis of the distribution of various electromorphs at different beta-glucosidase zones was carried out in natural populations of a. nidulans, the a. nidulans group, and various species belonging to the genus aspergillus from diverse geographical areas of india. the data show the existence of three segregating zones for beta-glucosidase, designated beta-glui, beta-gluii, and beta-gluiii. all three zones are present in wild isolates of a. nidulans, and only two, i.e., beta-glui ...	1988	3145736
glycerol uptake mutants of the hyphal fungus aspergillus nidulans.	a new class of glycerol non-utilizing mutants, designated glcc, has been isolated. the glcc gene was mapped in linkage group vi and mutants were found to complement the reference strains glca1 (linkage group v) and glcb33 (linkage group i) in diploids. the new mutants were unable to grow on glycerol. however, in contrast to the glca and glcb phenotype these mutants did grow well on dihydroxyacetone and d-galacturonate. by in vivo 13c nmr spectroscopy it was shown that the glcc mutant did not tak ...	1988	3053975
isolation and characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of aspergillus nidulans.	the isolation and characterization of the highly expressed glyceraldehyde-3-phosphate dehydrogenase (gpd)-coding gene (gpda) of aspergillus nidulans is described. the gene was isolated from an a. nidulans lambda gene library with a saccharomyces cerevisiae gpd-coding gene as a probe. unlike many other eukaryotes, a. nidulans contains only one gpd-coding gene. at the amino acid level, homology with other gpd enzymes is extensive. the a. nidulans gene contains seven introns, one of which is positi ...	1988	3066699
the ethanol regulon in aspergillus nidulans: characterization and sequence of the positive regulatory gene alcr.	the regulatory gene, alcr, of aspergillus nidulans, encodes a protein that induces the expression of the alca and alda genes. the alcr gene is inducible, autoregulated, and subject to carbon catabolite repression. we report the complete nucleotide sequence of the alcr gene and its 5' and 3' non-coding regions. in the 5' flanking region of the alcr gene, several repeats and inverted repeats were found, and small sequence similarities were also found with the 5' flanking regions of the alca and al ...	1988	3072264
induction of chromosome malsegregation by halogenated organic solvents in aspergillus nidulans: unspecific or specific mechanism?	three chloromethanes (dichloromethane, chloroform and carbon tetrachloride) and 8 chlorinated ethanes (1,1- and 1,2-dichloroethane, 1,1,1- and 1,1,2-trichloroethane, 1,1,1,2- and 1,1,2,2-tetrachloroethane, pentachloroethane and hexachloroethane) were assayed in tests for the induction of mitotic segregation in aspergillus nidulans diploid strain p1. eight of the 11 compounds assayed (dichloromethane, chloroform, carbon tetrachloride, 1,1- and 1,2-dichloroethane, 1,1,2-trichloroethane, 1,1,1,2- a ...	1988	3050490
aspergillus in pulmonary infections.		1988	3133316
cell-cycle modulation of mpm-2-specific spindle pole body phosphorylation in aspergillus nidulans.	mpm-2 is a monoclonal antibody that interacts with mitosis-specific phosphorylated proteins in many different organisms. immunocytochemistry of tissue culture cells has shown that mpm-2 stains centrosomes, chromosomes, kinetochores, and spindles. in this paper, we demonstrate that mpm-2 staining colocalizes with the spindle pole body (spb) of aspergillus nidulans and that spb staining varies during the mitotic cycle. in an unsynchronized population, about one-fourth to one-third of the cells sta ...	1988	3052873
isolation of a phytoalexin-detoxification gene from the plant pathogenic fungus nectria haematococca by detecting its expression in aspergillus nidulans.	detoxification of the pea phytoalexin pisatin via demethylation, mediated by a cytochrome p-450 monooxygenase, is thought to be important for pathogenicity of the fungus nectria haematococca on pea. to isolate a fungal gene encoding pisatin demethylating activity (pda), we transformed aspergillus nidulans with a genomic library of n. haematococca dna constructed in a cosmid which carried the a. nidulans trpc gene. transformants were selected for trp+ and then screened for pda. one transformant a ...	1988	3065148
unusual evolutionary conservation of 5s rrna pseudogenes in aspergillus nidulans: similarity of the dna sequence associated with the pseudogenes with the mouse immunoglobulin switch region.	all aspergillus nidulans 5s rrna pseudogenes known so far are the result of integration of an approx. 0.2-kbp-long dna sequence into the 5s rrna genes. this sequence, called block c, is present in at least five copies in the a. nidulans genome and seems to be associated either with 5s rrna genes or pseudogenes. in contrast to the 78% sequence conservation of the c-block in pseudogenes, the truncated 5' halves of the pseudogenes are very highly conserved (96.9-100%). we postulate that the 5s rrna ...	1988	3148732
a phosphate-repressible acid phosphatase gene from aspergillus niger: its cloning, sequencing and transcriptional analysis.	the cloning and sequencing of an aspergillus niger gene encoding a secreted form of phosphate-repressible acid phosphatase by complementation of a paca (phosphate-repressible acid phosphatase) mutant of aspergillus nidulans is described. the gene contains two introns, 201 and 265 nt in length, and codes for a 1.6-kb transcript. both phosphate concentration and ph of the growth medium affect the level of expression of the gene in a. niger. similar regulation is observed in a. nidulans transforman ...	1988	3224828
microorganisms associated with mouldiness of dried yam chips and their prevention.	the broad objective of this study was to isolate and identify the microorganisms causing mouldiness of stored yam chips and to look for ways of preventing the problem. microorganisms isolated included aspergillus flavus, a. glaucus, a. nidulans, a. niger, a. ochraceous, a. tamarii, a. candidus, penicillium oxalicum, trichoderma longibrachyatum, rhizopus nigricans, cylindrocarpon radicicola, neurospora crassa, botryodiplodia theobromae, bacillus subtilis, bacillus cereus, erwinia carotovora and s ...	1988	3231261
prevalence of airborne aspergillus flavus in khartoum (sudan) airspora with reference to dusty weather and inoculum survival in simulated summer conditions.	khartoum air was scanned for airborne aspergillus flavus for 12 months using the horizontal gravitational settling method. frequency of occurrence was related to total fungal catch and dusty weather. the aspergilli were prevalent (68% of total isolated/plate/month) and a. flavus constituted 31% of the total aspergilli. in june (hot, dry & dusty) aspergilli constituted 79% of the total isolates, whilst a. flavus represented 30% from amongst the other aspergilli. a. flavus, a. niger, a. nidulans ( ...	1988	3148861
ammonium ion sensitivity is a ribosomal phenotype associated with suppressor mutations in the suac gene of aspergillus nidulans.	ammonium ions are selectively toxic to strains containing mutations in the suac gene which can mutate to a suppressor phenotype. this phenotype is associated with increased ribosomal misreading in vitro (zamir and martinelli 1987) and altered ribosomal proteins (harvey and martinelli 1983). such ammonium-sensitivity is a feature of both strong and weak suppressor alleles, and segregates with suppressor ability in crosses. suppressor mutations in the suab and suad genes are not affected, nor are ...	1988	3066509
an adaptive response to alkylating agents in aspergillus nidulans.	a simple method is described for demonstrating adaptation to alkylation damage in aspergillus nidulans. one wild type, two mnng-sensitive, and one mnng-resistant strain all showed improvement in colony growth when challenged with mnng following appropriate inducing pretreatments. other alkylating agents (mms, ems) could also adapt mycelium to later mnng challenge, while 4nqo and uv could not. the inducible effect was not transmissible through conidia. a standard reversion assay based upon methg ...	1988	3066510
on the mutagenic and recombinogenic activity of certain herbicides in salmonella typhimurium and in aspergillus nidulans.	the plant growth-regulating hormones indole-3-acetic acid (iaa) and indole-3-butyric acid (iba), both strong recombinogens in aspergillus nidulans, were tested in salmonella typhimurium strains for his revertants at a range of concentrations from 1 to 2000 micrograms/plate with and without metabolic activation and were found negative. also 3 herbicides of the chlorophenoxy group, 2,4-(dichlorophenoxy)acetic acid (2,4-d), 2,4-(dichlorophenoxy)butyric acid (2,4-db) and 4-chloro-2-methylphenoxyacet ...	1988	3280995
characterization of a cyanobacterial iron stress-induced gene similar to psbc.	recently we have reported that the flavodoxin gene from the cyanobacterium anacystis nidulans r2 is transcribed as part of an iron stress-induced operon containing multiple mrna species (d. e. laudenbach, m. e. reith, and n. a. straus, j. bacteriol. 170: 258-265, 1988). here we report that nucleotide sequence analyses of dna located immediately upstream of the flavodoxin gene revealed an open reading frame of 1,026 bases (designated isia; iron stress inducible) with a deduced amino acid sequence ...	1988	3141374
transformation of penicillium chrysogenum using dominant selection markers and expression of an escherichia coli lacz fusion gene.	an industrial penicillium chrysogenum strain was transformed using two dominant selection markers, namely the bacterial gene for phleomycin resistance (ble) fused to a fungal promoter, and the acetamidase (amds) gene from aspergillus nidulans. transformation frequencies of up to 20 transformants per microgram of dna were obtained with the ble system. with the amds marker the frequency was up to 120 transformants. cotransformation was very efficient when using amds as a selection marker. the intr ...	1988	3131191
transformation of penicillium chrysogenum with a dominant selectable marker.	we have cloned a mutant oligomycin resistance allele of the mitochondrial atp synthase subunit 9 gene from the filamentous fungus penicillium chrysogenum. the gene was isolated using the equivalent gene from aspergillus nidulans as a hybridisation probe. using the cloned gene it is possible to select for oligomycin resistance in p. chrysogenum transformation experiments. this transformation system was used to introduce further copies of the p. chrysogenum isopenicillin n synthetase gene, which w ...	1988	3135949
comparative study on the evolution of chloroplast ribosomal 5s rna of a living fossil plant, cycas revoluta thumb.	the complete nucleotide sequence of cycas revoluta thunb chloroplast 5 s rrna was determined. it consists of 122 nucleotides. this is the only known 5 s rrna sequence in gymnospermae. it is highly homologous with chloroplast 5 s rrna of higher plants (92-97%), but less homologous (about 54%) with those of lower plants. there is however 67% homology between cycas and a procaryote a. nidulans. the chloroplast 5 s rrnas of angiospermae are nearly identical with each other (95-97%). s. oligorhize an ...	1988	3136036
the amidases from a brevibacterium strain: study and applications.		1988	3142225
changes in sulfate transport characteristics and protein composition of anacystis nidulans r2 during sulfur deprivation.	sulfur-starved cells of anacystis nidulans have an increased capacity to take up sulfate. the apparent vmax for sulfate uptake increased at least 10-fold after 24 h of sulfur deprivation, whereas the k1/2 remained unchanged at approximately 1.35 microm. the initial rate of sulfate uptake increased between 2 and 6 h after transfer of the cells to sulfur-free medium, in concert with elevated levels of three cytoplasmic membrane polypeptides with molecular masses of 43, 42, and 36 kilodaltons (kda) ...	1988	3123460
isolation, sequence analysis, and transcriptional studies of the flavodoxin gene from anacystis nidulans r2.	the nonheme, iron-sulfur protein ferredoxin is the terminal constituent of the photosynthetic electron transport chain. under conditions of iron stress, many cyanobacteria and eucaryotic algae replace ferredoxin with the flavoprotein flavodoxin. the gene for flavodoxin was cloned from the cyanobacterium anacystis nidulans r2 by using three mixed oligonucleotide probes derived from the partial synechococcus sp. strain pcc 6301 amino acid sequence. nucleotide sequence analysis revealed a 513-base- ...	1988	3121586
genetic regulation of development in aspergillus nidulans.		1988	3076298
aspergillus nidulans contains a single actin gene which has unique intron locations and encodes a gamma-actin.	the single actin gene from the filamentous fungus aspergillus nidulans has been isolated and characterized. the only other organism reported to contain just one actin gene is another ascomycete, the budding yeast saccharomyces. the nucleotide sequence of the a. nidulans actin gene predicts a polypeptide containing the n-terminal sequence identifying the gamma-actin isotype. until now this characteristic n terminus has only been reported to occur in vertebrate actin sequences. a monospecific anti ...	1988	2975248
genetic regulation of the quinic acid utilization (qut) gene cluster in aspergillus nidulans.	a large number of quinic acid non-utilizing qut mutants of aspergillus nidulans deficient in the induction of all three quinic acid specific enzymes have been analysed. one class the qutd mutants, are all recessive and are non-inducible at ph 6.5 due to inferred deficiency in a quinate ion permease. two regulatory genes have been identified. the quta gene encodes an activator protein since most quta mutants are recessive and non-inducible although a few fully dominant mutants have been found. th ...	1988	3049934
molecular cloning, identification and transcriptional analysis of genes involved in acetate utilization in neurospora crassa.	four neurospora crassa genomic clones have been selected as hybridizing much more strongly to labelled mrna isolated from acetate-grown mycelium than to mrna from sucrose-grown mycelium. hybridization of restriction fragments with acetate-specific mrna or cdna has been used to delimit the transcribed region(s) of each clone. the transcription of all four clones is strongly induced by transfer of growing mycelium from sucrose to acetate as sole carbon source. in wild-type mycelium, mrnas correspo ...	1988	3054423
location and biosynthetic regulation of endo-1,4-beta-glucanase in aspergillus nidulans.	the location and biosynthetic regulation of endo-1,4-beta-glucanase were studied in aspergillus nidulans. the enzyme was found to be extracellular, but low intracellular activity was also detected at the beginning of enzyme induction. the synthesis of the enzyme was regulated by carbon catabolite repression as well as specific induction. beta-1,4-linked carbohydrates, such as carboxymethylcellulose, cellobiose and lactose, induced the enzyme synthesis, while 2-deoxyglucose and readily metaboliza ...	1988	3054436
cloning and analysis of the positively acting regulatory gene amdr from aspergillus nidulans.	the positively acting regulatory gene amdr of aspergillus nidulans coordinately regulates the expression of four unlinked structural genes involved in acetamide (amds), omega amino acid (gata and gaba), and lactam (lama) catabolism. by the use of dna-mediated transformation of a. nidulans, the amdr regulatory gene was cloned from a genomic cosmid library. southern blot analysis of dna from various loss-of-function amdr mutants revealed the presence of four detectable dna rearrangements, includin ...	1988	3062382
evaluation of the mutagenic activity of leucinostatins, a novel class of antibiotic peptides produced by paecilomyces marquandii, in the modul aspergillus nidulans.	leucinostatins a, b, c, d, e, g, h, and k were thoroughly investigated for their genotoxic activity using the modul aspergillus nidulans as the test organism. the results of assays for gene mutation (8-azaguanine resistance and methionine suppressors), gene conversion, mitotic crossing-over and mitotic aneuploidy induction suggest that these peptide antibiotics lack significant mutagenicity and that non-genotoxic mechanism(s) underlie their cytotoxic properties.	1988	3063923
mitotic gene conversion, reciprocal recombination and gene replacement at the bena, beta-tubulin, locus of aspergillus nidulans.	we have developed a procedure for determining the rates of mitotic recombination of an interrupted duplication created by integration of transforming plasmid sequences at the bena, beta-tubulin, locus of aspergillus nidulans. transformation of a strain carrying a benomyl-resistant bena allele with plasmid aipgm4, which carries the wild-type bena allele and the pyr4 (orotidine-5'-phosphate decarboxylase) gene of neurospora crassa, creates an interrupted duplication with plasmid sequences flanked ...	1988	3054484
localisation of several chromosome i genes of aspergillus nidulans: implications for mitotic recombination.	another laboratory previously reported that the vast majority of mitotic recombinants in chromosome i disomics of aspergillus nidulans arise from double exchange events involving the centromeric region and a far distal, possibly telomeric, region. this conclusion was based on the assumption that the camc gene is located in a position far distal to the centromere on the left arm of chromosome i. as a left arm location for camc distal to the centromere was possibly in conflict with mapping data ob ...	1988	3054488
a rapid purification procedure for pyruvate kinase from the hyphal fungus aspergillus nidulans.	pyruvate kinase was purified from the filamentous fungus aspergillus nidulans with a 45-55% yield. the procedure involved dye-affinity chromatography and fast protein liquid chromatography, resulting in highly active and pure enzyme in milligram quantities within 2 days. the purified enzyme, a tetramer with a subunit molecular weight of 65,000 and an isoelectric point of 4.7, was used to determine the amino acid composition.	1988	3058273
chronic granulomatous disease of childhood. an unusual case of infection with aspergillus nidulans var. echinulatus.	aspergillus nidulans var. echinulatus was the sole agent cultured from the left lung, a paraspinal abscess, left ribs, and thoracic vertebral bodies from a patient with chronic granulomatous disease. hyphal elements were present in histologic sections of lung, vertebral bodies, and infected ribs along with granuloma formation. the patient was treated with two debridement procedures and insertion of a harrington rod followed by a long course of amphotericin b, flucytosine, and daily white blood c ...	1988	3046321
behaviour of a replicating mitochondrial dna sequence from aspergillus amstelodami in saccharomyces cerevisiae and aspergillus nidulans.	an amplified sequence of mitochondrial dna from a ragged (rgd) mutant of aspergillus amstelodami has been shown to exist in multimeric circular form, suggesting that it is excised from the genome and can exist independently of it. this sequence has replicative (ars) activity in saccharomyces cerevisiae, and a subfragment responsible for this activity has been identified and sequenced. a homologous sequence from aspergillus nidulans mtdna also has ars activity in s. cerevisiae. both a. amstelodam ...	1988	3042169