PMID(sorted descending)
the aspergillus nidulans nuca(endog) homologue is not involved in cell death.upon apoptosis induction, translocation of mammalian mitochondrial endonuclease g (endog) to the nucleus coincides with large-scale dna fragmentation. here, we describe for the first time a homologue of endog in filamentous fungi by investigating if the aspergillus nidulans homologue of the endog gene, named nuca(endog), is being activated during farnesol-induced cell death. our results suggest that nuca is not involved in cell death, but it plays a role in the dna-damaging response in a. nidula ...201021131437
hapx positively and negatively regulates the transcriptional response to iron deprivation in cryptococcus neoformans.the fungal pathogen cryptococcus neoformans is a major cause of illness in immunocompromised individuals such as aids patients. the ability of the fungus to acquire nutrients during proliferation in host tissue and the ability to elaborate a polysaccharide capsule are critical determinants of disease outcome. we previously showed that the gata factor, cir1, is a major regulator both of the iron uptake functions needed for growth in host tissue and the key virulence factors such as capsule, melan ...201021124817
recruitment of the inhibitor cand1 to the cullin substrate adaptor site mediates interaction to the neddylation site.cand1 inhibits cullin ring ubiquitin ligases by binding unneddylated cullins. the cand1 n-terminus blocks the cullin neddylation site, whereas the c-terminus inhibits cullin adaptor interaction. these cand1 binding sites can be separated into two functional polypeptides which bind sequentially. c-terminal cand1 can directly bind to unneddylated cullins in the nucleus without blocking the neddylation site. the smaller n-terminal cand1 cannot bind to the cullin neddylation region without c-termina ...201021119001
homologue expression of a β-xylosidase from native aspergillus niger.xylan constitutes the second most abundant source of renewable organic carbon on earth and is located in the cell walls of hardwood and softwood plants in the form of hemicellulose. based on its availability, there is a growing interest in production of xylanolytic enzymes for industrial applications. β-1,4-xylan xylosidase (ec hydrolyses from the nonreducing end of xylooligosaccharides arising from endo-1,4-β-xylanase activity. this work reports the partial characterization of a purif ...201021116681
involvement of the aspergillus nidulans protein kinase c with farnesol tolerance is related to the unfolded protein response.previously, we demonstrated that the aspergillus nidulans calc2 mutation in protein kinase c pkca was able to confer tolerance to farnesol (foh), an isoprenoid that has been shown to inhibit proliferation and induce apoptosis. here, we investigate in more detail the role played by a. nidulans pkca in foh tolerance. we demonstrate that pkca overexpression during foh exposure causes increased cell death. foh is also able to activate several markers of endoplasmic reticulum (er) stress and the unfo ...201021091509
conserved components, but distinct mechanisms for the placement and assembly of the cell division machinery in unicellular and filamentous ascomycetes.cytokinesis is essential for cell proliferation, yet its molecular description is challenging, because >100 conserved proteins must be spatially and temporally co-ordinated. despite the high importance of a tight co-ordination of cytokinesis with chromosome and organelle segregation, the mechanism for determining the cell division plane is one of the least conserved aspects of cytokinesis in eukaryotic cells. budding and fission yeast have developed fundamentally distinct mechanisms to ensure pr ...201021091496
penicillium marneffei actin expression during phase transition, oxidative stress, and macrophage infection.penicillium marneffei is an opportunistic fungal pathogen that exhibits thermally regulated dimorphism. at 25°c, this fungus grows vegetatively as mycelia, but at 37°c or upon invasion of a host, a fission yeast form is established. yet, despite increased numbers of molecular studies involving this fungus, the role of p. marneffei stress response-related proteins is not well characterized. actin is one of the proteins that have been proposed to play a role not only in cell transition, but also i ...201021088905
human leukocytes kill aspergillus nidulans by reactive oxygen species-independent mechanisms.invasive aspergillosis is a major threat for patients suffering from chronic granulomatous disease (cgd). although aspergillus fumigatus is the most commonly encountered aspergillus species, the presence of a. nidulans appears to be disproportionately high in cgd patients. the purpose of this study was to investigate the involvement of the nadph oxidase and the resulting reactive oxygen species (ros) in host defense against fungi and to clarify their relationship toward a. nidulans. murine cgd a ...201021078850
type 2c protein phosphatases in fungi.type 2c ser/thr phosphatases are a remarkable class of protein phosphatases, which are conserved in eukaryotes and involved in a large variety of functional processes. unlike in other ser/thr phosphatases, the catalytic polypeptide is not usually associated with regulatory subunits, and functional specificity is achieved by encoding multiple isoforms. for fungi, most information comes from the study of type 2c protein phosphatase (pp2c) enzymes in saccharomyces cerevisiae, where seven pp2c-encod ...201021076010
the cop9 signalosome mediates transcriptional and metabolic response to hormones, oxidative stress protection and cell wall rearrangement during fungal development.the cop9 signalosome complex (csn) is a crucial regulator of ubiquitin ligases. defects in csn result in embryonic impairment and death in higher eukaryotes, whereas the filamentous fungus aspergillus nidulans survives without csn, but is unable to complete sexual development. we investigated overall impact of csn activity on a. nidulans cells by combined transcriptome, proteome and metabolome analysis. absence of csn5/csne affects transcription of at least 15% of genes during development, inclu ...201021062371
aspergillus nidulans asexual development: making the most of cellular modules.asexual development in aspergillus nidulans begins in superficial hyphae as the programmed emergence of successive pseudohyphal modules, collectively known as the conidiophore, and is completed by a layer of specialized cells (phialides) giving rise to chains of aerial spores. a discrete number of regulatory factors present in hyphae play different stage-specific roles in pseudohyphal modules, depending on their cellular localization and protein-protein interactions. their multiple roles include ...201021035346
in vitro susceptibility testing of aspergillus spp. against voriconazole, itraconazole, posaconazole, amphotericin b and caspofungin.during recent years, the incidence of serious infections caused by opportunistic fungi has increased dramatically due to alterations of the immune status of patients with hematological diseases, malignant tumors, transplantations and so forth. unfortunately, the wide use of triazole antifungal agents to treat these infections has lead to the emergence of aspergillus spp. resistant to triazoles. the present study was to assess the in vitro activities of five antifungal agents (voriconazole, itrac ...201021034656
an ortholog of fara of aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast yarrowia lipolytica.the yeast yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes. to identify a gene(s) regulating fatty acid utilization in y. lipolytica, we first studied homologous genes to oaf1 and pip2 of saccharomyces cerevisiae, but their disruption did not change growth on oleic acid at all. we next characterized a y. lipolytica gene, por1 (primary oleate regulator 1), an ortholog of fara encoding a transcriptional activator that regulates fatty acid utilizatio ...201020977884
microbiological analysis of a mummy from the archeological museum in this paper we report the results of the microbiological analysis of the samples taken from the mummy from the collection of the archaeological museum in zagreb, croatia. samples were taken from specific places such as oral, orbital, abdominal cavity and bandages surrounding the mummy, and analyzed in department of microbiology and hospital infections in university hospital "dubrava" in zagreb and in national reference laboratory for systemic mycoses of croatian national institute of public he ...201020977065
transcriptome analysis of nitrate assimilation in aspergillus nidulans reveals connections to nitric oxide metabolism.nitrate is a dominant form of inorganic nitrogen (n) in soils and can be efficiently assimilated by bacteria, fungi and plants. we studied here the transcriptome of the short-term nitrate response using assimilating and non-assimilating strains of the model ascomycete aspergillus nidulans. among the 72 genes positively responding to nitrate, only 18 genes carry binding sites for the pathway-specific activator nira. forty-five genes were repressed by nitrate metabolism. because nira(-) strains ar ...201020969648
pectin lyase production by recombinant penicillium griseoroseum strain 105.recombinant penicillium griseoroseum strain 105 overproduces an extracellular pectin lyase (pl) under the transcriptional control of the strong gpda promoter of aspergillus nidulans. our aim was to evaluate pl production by recombinant p. griseoroseum strain 105 in submerged fermentation system bioreactors biofloiii and biofloiv using 2 or 10 l working volumes under different growth conditions and to analyze the production of cellulase, polygalacturonase, pectin methylesterase, and protease. pl ...201020962906
novel mutations reveal two important regions in aspergillus nidulans transcriptional activator metr.expression of the sulfur assimilation pathway in aspergillus nidulans is under control of sulfur metabolite repression, which is composed of scon genes encoding subunits of ubiquitin ligase and the metr gene coding for a transcriptional activator. in this paper we report three dominant suppressors of methionine requirement isolated from a metb3 diploid strain. all three mutations lead to the substitution of phenylalanine 48 by serine or leucine in the conserved n-terminal region of the metr prot ...201020955810
activation of a silent fungal polyketide biosynthesis pathway through regulatory cross talk with a cryptic nonribosomal peptide synthetase gene cluster.filamentous fungi produce numerous natural products that constitute a consistent source of potential drug leads, yet it seems that the majority of natural products are overlooked since most biosynthesis gene clusters are silent under standard cultivation conditions. screening secondary metabolite genes of the model fungus aspergillus nidulans, we noted a silent gene cluster on chromosome ii comprising two nonribosomal peptide synthetase (nrps) genes, inpa and inpb, flanked by a regulatory gene t ...201020952652
functional characterization of a new member of the cdk9 family in aspergillus nidulans.cdk9-like kinases in complex with t-type cyclins are essential components of the eukaryotic transcription elongation machinery. the full spectrum of cdk9/cyclin t targets, as well as the specific consequences of phosphorylations, is still largely undefined. we identify and characterize here a cdk9 kinase (ptka) in the filamentous ascomycete aspergillus nidulans. deletion of ptka had a lethal effect in later stages of vegetative growth and completely impeded asexual development. overexpression of ...201020952582
fungal tyrosine betaine, a novel secondary metabolite from conidia of entomopathogenic metarhizium spp. fungi.fungi, including the entomopathogenic deuteromycete metarhizium anisopliae, produce a wide diversity of secondary metabolites that either can be secreted or stored in specific developmental structures, e.g., conidia. some secondary metabolites, such as pigments, polyols and mycosporines, are associated with pathogenicity and/or fungal tolerance to several stress-inducing environmental factors, including temperature and solar radiation extremes. extracts of m. anisopliae var. anisopliae (strain e ...201020943158
hapx-mediated adaption to iron starvation is crucial for virulence of aspergillus fumigatus.iron is essential for a wide range of cellular processes. here we show that the bzip-type regulator hapx is indispensable for the transcriptional remodeling required for adaption to iron starvation in the opportunistic fungal pathogen aspergillus fumigatus. hapx represses iron-dependent and mitochondrial-localized activities including respiration, tca cycle, amino acid metabolism, iron-sulfur-cluster and heme biosynthesis. in agreement with the impact on mitochondrial metabolism, hapx-deficiency ...201020941352
suppressor mutagenesis identifies a velvet complex remediator of aspergillus nidulans secondary metabolism.fungal secondary metabolites (sm) are bioactive compounds that are important in fungal ecology and, moreover, both harmful and useful in human endeavors (e.g., as toxins and pharmaceuticals). recently a nuclear heterocomplex termed the velvet complex, characterized in the model ascomycete aspergillus nidulans, was found to be critical for sm production. deletion of two members of the velvet complex, laea and vea, results in near loss of sm and defective sexual spore production in a. nidulans and ...201020935144
fungal development and the cop9 signalosome.the conserved cop9 signalosome (csn) multiprotein complex is located at the interface between cellular signaling, protein modification, life span and the development of multicellular organisms. csn is required for light-controlled responses in filamentous fungi. this includes the circadian rhythm of neurospora crassa or the repression of sexual development by light in aspergillus nidulans. in contrast to plants and animals, csn is not essential for fungal viability. therefore fungi are suitable ...201020934903
hypertonic conditions trigger transient plasmolysis, growth arrest and blockage of transporter endocytosis in aspergillus nidulans and saccharomyces using aspergillus nidulans strains expressing functional gfp-tagged transporters under hypertonic conditions, we noticed the rapid appearance of cortical, relatively static, fluorescent patches (0.5-2.3 μm). these patches do not correspond to transporter microdomains as they co-localize with other plasma membrane-associated molecules, such as the pleckstrin homology (ph) domain and the ssoa t-snare, or the lipophilic markers fm4-64 and filipin. in addition, they do not show characteristics of ...201020919858
global gene expression analysis of aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia.hypoxia imposes a challenge upon most filamentous fungi that require oxygen for proliferation. here, we used whole genome dna microarrays to investigate global transcriptional changes in aspergillus nidulans gene expression after exposure to hypoxia followed by normoxia. aeration affected the expression of 2,864 genes (27% of the total number of genes in the fungus), of which 50% were either induced or repressed under hypoxic conditions. up-regulated genes included those for glycolysis, ethanol ...201020878186
the microtubule plus-end localization of aspergillus dynein is important for dynein-early-endosome interaction but not for dynein atpase activation.cytoplasmic dynein in filamentous fungi accumulates at microtubule plus-ends near the hyphal tip, which is important for minus-end-directed transport of early endosomes. it was hypothesized that dynein is switched on at the plus-end by cargo association. here, we show in aspergillus nidulans that kinesin-1-dependent plus-end localization is not a prerequisite for dynein atpase activation. first, the walker a and walker b mutations in the dynein heavy chain aaa1 domain implicated in blocking diff ...201020876661
quantification of cyclobutane pyrimidine dimers induced by uvb radiation in conidia of the fungi aspergillus fumigatus, aspergillus nidulans, metarhizium acridum and metarhizium robertsii.conidia are responsible for reproduction, dispersal, environmental persistence and host infection of many fungal species. one of the main environmental factors that can kill and/or damage conidia is solar uv radiation. cyclobutane pyrimidine dimers (cpd) are the major dna photoproducts induced by uvb. we examined the conidial germination kinetics and the occurrence of cpd in dna of conidia exposed to different doses of uvb radiation. conidia of aspergillus fumigatus, aspergillus nidulans and met ...201020860693
aspergillus fumigatus flbb encodes two basic leucine zipper domain (bzip) proteins required for proper asexual development and gliotoxin production.the opportunistic human pathogen aspergillus fumigatus reproduces asexually by forming a massive number of mitospores called conidia. in this study, we characterize the upstream developmental regulator a. fumigatus flbb (afuflbb). northern blotting and cdna analyses reveal that afuflbb produces two transcripts predicted to encode two basic leucine zipper domain (bzip) polypeptides, afuflbbβ (420 amino acids [aa]) and afuflbbα (390 aa). the deletion of afuflbb results in delayed/reduced sporulati ...201020852021
mitotic recombination: a genotoxic effect of the antidepressant citalopram in aspergillus nidulans.this report evaluates the potential of the antidepressant drug citalopram to induce homozygotization of genes previously present in a heterozygous condition, by homologous recombination. in order to address this question, a heterozygous diploid strain of the filamentous fungus aspergillus nidulans and the homozygotization assay were utilized. non-cytotoxic concentrations of citalopram (50, 75 and 100 μmol/l) showed a strong recombinogenic effect in a. nidulans, inducing homozygosis of the diploi ...201020851831
screening for antifungal peptides and their modes of action in aspergillus nidulans.many short cationic peptides have been identified as potent antimicrobial agents, but their modes of action are not well understood. peptide synthesis on cellulose membranes has resulted in the generation of peptide libraries, while high-throughput assays have been developed to test their antibacterial activities. in this paper a microtiter plate-based screening method for fungi has been developed and used to test nine antibacterial peptides against the model fungus aspergillus nidulans. microsc ...201020833782
analysis of intact and dissected fungal polyketide synthase-nonribosomal peptide synthetase in vitro and in saccharomyces cerevisiae.the widely found fungal iterative pks-nrps hybrid megasynthetases are highly programmed biosynthetic machines involved in the synthesis of 3-acyltetramic acids and related natural products. in vitro analysis of iterative pks-nrps has been hampered by the difficulties associated with obtaining pure and functional forms of these large enzymes (>400 kda). we successfully expressed aspergillus nidulans aspyridone synthetase (apda) from an engineered saccharomyces cerevisiae strain. the complete func ...201020828130
characterization of the developmental regulator flbe in aspergillus fumigatus and aspergillus nidulans.several upstream developmental activators control asexual development (conidiation) in aspergillus. in this study, we characterize one of such activators called flbe in aspergillus fumigatus and aspergillus nidulans. the predicted flbe protein is composed of 222 and 201 aa in a. fumigatus and a. nidulans, respectively. while flbe is transiently expressed during early phase of growth in a. nidulans, it is somewhat constitutively expressed during the lifecycle of a. fumigatus. the deletion of flbe ...201020817115
cross-talk between light and glucose regulation controls toxin production and morphogenesis in aspergillus nidulans.light is a major environmental stimulus that has a broad effect on organisms, triggering a cellular response that results in an optimal adaptation enhancing fitness and survival. in fungi, light affects growth, and causes diverse morphological changes such as those leading to reproduction. light can also affect fungal metabolism, including the biosynthesis of natural products. in this study we show that in aspergillus nidulans the effect of light on the production of the sterigmatocystin (st) to ...201020816830
comparison of transcriptional and translational changes caused by long-term menadione exposure in aspergillus nidulans.under long-term oxidative stress caused by menadione sodium bisulfite, genome-wide transcriptional and proteome-wide translational changes were compared in aspergillus nidulans vegetative cells. the comparison of proteomic and dna microarray expression data demonstrated that global gene expression changes recorded with either flip-flop or dendrimer cdna labeling techniques supported proteome changes moderately with 40% and 34% coincidence coefficients, respectively. enzyme levels in the glycolyt ...201120797444
repercussion of a deficiency in mitochondrial ß-oxidation on the carbon flux of short-chain fatty acids to the peroxisomal ß-oxidation cycle in aspergillus nidulans.the fungus aspergillus nidulans contains both a mitochondrial and peroxisomal ß-oxidation pathway. this work was aimed at studying the influence of mutations in the foxa gene, encoding a peroxisomal multifunctional protein, or in the scda/echa genes, encoding a mitochondrial short-chain dehydrogenase and an enoyl-coa hydratase, respectively, on the carbon flux to the peroxisomal ß-oxidation pathway. a. nidulans transformed with a peroxisomal polyhydroxyalkanoate (pha) synthase produced pha from ...201020736083
the bzip transcription factor meab mediates nitrogen metabolite repression at specific fusarium fujikuroi, bikaverin (bik) biosynthesis is subject to repression by nitrogen. unlike most genes subject to nitrogen metabolite repression, it has been shown that transcription of bik biosynthetic genes is not area dependent. searching for additional transcription factors that may be involved in nitrogen regulation, we cloned and characterized the orthologue of aspergillus nidulans meab, which encodes a bzip transcription factor. two transcripts are derived from f. fujikuroi meab: the ...201020729292
telomere position effect is regulated by heterochromatin-associated proteins and nkua in aspergillus nidulans.gene-silencing mechanisms are being shown to be associated with an increasing number of fungal developmental processes. telomere position effect (tpe) is a eukaryotic phenomenon resulting in gene repression in areas immediately adjacent to telomere caps. here, tpe is shown to regulate expression of transgenes on the left arm of chromosome iii and the right arm of chromosome vi in aspergillus nidulans. phenotypes found to be associated with transgene repression included reduction in radial growth ...201020724388
the human ku autoantigen shares amino acid sequence homology with fungal, but not bacterial and viral, proteins.context: molecular mimicry between autoantigens and microbial antigens is a possible triggering mechanism of autoimmunity. human ku is a dna-associated autoantigen targeted by autoantibodies in patients with systemic lupus erythematosus and related disorders; available data are consistent with a role of molecular mimicry in the pathogenesis of anti-ku autoimmunity, but no research exist on this topic. objective: we aimed to define the most probable microbial triggers of anti-ku autoimmunity via ...201120718578
characterization of the aspergillus nidulans biotin biosynthetic gene cluster and use of the bioda gene as a new transformation marker.the genes involved in the biosynthesis of biotin were identified in the hyphal fungus aspergillus nidulans through homology searches and complementation of escherichia coli biotin-auxotrophic mutants. whereas the 7,8-diaminopelargonic acid synthase and dethiobiotin synthetase are encoded by distinct genes in bacteria and the yeast saccharomyces cerevisiae, both activities are performed in a. nidulans by a single enzyme, encoded by the bifunctional gene bioda. such a bifunctional bioda gene is a ...201120713166
inadvertent gene silencing of argininosuccinate synthase (bcass1) in botrytis cinerea by the plob1 vector system.for several years, researchers working on the plant pathogen botrytis cinerea and a number of other related fungi have routinely used the plob1 vector system, based on hygromycin resistance, under the control of the aspergillus nidulans olic promoter and what was reported to be the beta-tubulin (tuba) terminator. recently, it has been demonstrated that this vector contains a 446-bp portion of the b. cinerea argininosuccinate synthase gene (bcass1) rather than the tuba terminator. as argininosucc ...201020696000
endocytic machinery protein slab is dispensable for polarity establishment but necessary for polarity maintenance in hyphal tip cells of aspergillus nidulans.the aspergillus nidulans endocytic internalization protein slab is essential, in agreement with the key role in apical extension attributed to endocytosis. we constructed, by gene replacement, a nitrate-inducible, ammonium-repressible slab1 allele for conditional slab expression. video microscopy showed that repressed slab1 cells are able to establish but unable to maintain a stable polarity axis, arresting growth with budding-yeast-like morphology shortly after initially normal germ tube emerge ...201020693304
eisosome organization in the filamentous ascomycete aspergillus nidulans.eisosomes are subcortical organelles implicated in endocytosis and have hitherto been described only in saccharomyces cerevisiae. they comprise two homologue proteins, pil1 and lsp1, which colocalize with the transmembrane protein sur7. these proteins are universally conserved in the ascomycetes. we identify in aspergillus nidulans (and in all members of the subphylum pezizomycotina) two homologues of pil1/lsp1, pila and pilb, originating from a duplication independent from that extant in the su ...201020693301
evolutionary genetics: desperate times call for more sex.a new study has found that strains of the fungus aspergillus nidulans produce more of their spores sexually in environments where they are less fit, resembling a hypothesized transitional stage in the evolution of sex.201020692611
aspergillus nidulans alpha-galactosidase of glycoside hydrolase family 36 catalyses the formation of alpha-galacto-oligosaccharides by transglycosylation.the alpha-galactosidase from aspergillus nidulans (aglc) belongs to a phylogenetic cluster containing eukaryotic alpha-galactosidases and alpha-galacto-oligosaccharide synthases of glycoside hydrolase family 36 (gh36). the recombinant aglc, produced in high yield (0.65 g.l(-1) culture) as his-tag fusion in escherichia coli, catalysed efficient transglycosylation with alpha-(1-->6) regioselectivity from 40 mm 4-nitrophenol alpha-d-galactopyranoside, melibiose or raffinose, resulting in a 37-74% y ...201020681989
gamma-tubulin regulates the anaphase-promoting complex/cyclosome during interphase.a cold-sensitive gamma-tubulin allele of aspergillus nidulans, mipad159, causes defects in mitotic and cell cycle regulation at restrictive temperatures that are apparently independent of microtubule nucleation defects. time-lapse microscopy of fluorescently tagged mitotic regulatory proteins reveals that cyclin b, cyclin-dependent kinase 1, and the ancdc14 phosphatase fail to accumulate in a subset of nuclei at restrictive temperatures. these nuclei are permanently removed from the cell cycle, ...201020679430
a comparative study of activity and apparent inhibition of fungal β-glucosidases.β-glucosidases (bgs) from aspergillus fumigates, aspergillus niger, aspergillus oryzae, chaetomium globosum, emericella nidulans, magnaporthe grisea, neurospora crassa, and penicillium brasilianum were purified to homogeneity, and analyzed by isothermal titration calorimetry with respect to their hydrolytic activity and its sensitivity to glucose (product) using cellobiose as substrate. global non-linear regression of several reactions, with or without added glucose, to a product inhibition equa ...201020677177
the l279p mutation of nuclear distribution gene c (nudc) influences its chaperone activity and lissencephaly protein 1 (lis1) stability.lis1, a gene mutated in classical lissencephaly, plays essential roles in cytoplasmic dynein regulation, mitosis and cell migration. however, the regulation of lis1 (lissencephaly protein 1) protein remains largely unknown. genetic studies in aspergillus nidulans have uncovered that the nud (nuclear distribution) pathway is involved in the regulation of cytoplasmic dynein complex and a temperature-sensitive mutation in the nudc gene (l146p) greatly reduces the protein levels of nudf, an aspergil ...201020675372
the gata-type transcriptional activator gat1 regulates nitrogen uptake and metabolism in the human pathogen cryptococcus neoformans.nitrogen uptake and metabolism are essential to microbial growth. gat1 belongs to a conserved family of zinc finger containing transcriptional regulators known as gata-factors. these factors activate the transcription of nitrogen catabolite repression (ncr) sensitive genes when preferred nitrogen sources are absent or limiting. cryptococcus neoformans gat1 is an ortholog to the aspergillus nidulans area and candida albicans gat1 genes. in an attempt to define the function of this transcriptional ...201120673806
aspernidine a and b, prenylated isoindolinone alkaloids from the model fungus aspergillus nidulans. 201020661238
the roles played by aspergillus nidulans apoptosis-inducing factor (aif)-like mitochondrial oxidoreductase (aifa) and nadh-ubiquinone oxidoreductases (ndea-b and ndia) in farnesol resistance.farnesol (foh) is a nonsterol isoprenoid produced by dephosphorylation of farnesyl pyrophosphate, a catabolite of the cholesterol biosynthetic pathway. these isoprenoids inhibit proliferation and induce apoptosis. here, we show that aspergillus nidulans aifa encoding the apoptosis-inducing factor (aif)-like mitochondrial oxidoreductase plays a role in the function of the mitochondrial complex i. additionally, we demonstrated that ndea-b and ndia encode external and internal alternative nadh dehy ...201020654725
genome-wide analysis of microsatellite sequence in seven filamentous fungi.abundance of microsatellites with repeated unit lengths of 1-6 base pairs in seven fungi: aspergillus nidulans, coprinus cinereus, cryptococcus neoformans (serotype a), fusarium graminearum, magnaporthe grisea, neurospora crassa and ustilago maydis were investigated on genomic scale. the results showed that each species has its specific profile for different types and different motifs of ssr loci. ascomycetes fungi m. grisea, n. crassa and basidiomycete fungus u. maydis adopt much more microsate ...200920640828
urea, the major urea/h+ symporter in aspergillus nidulans.we report here the characterization of urea, a high-affinity urea/h+ symporter of aspergillus nidulans. the deletion of the encoding gene abolishes urea transport at low substrate concentrations, suggesting that in these conditions urea is the sole transport system specific for urea in a. nidulans. the urea gene is not inducible by urea or its precursors, but responds to nitrogen metabolite repression, necessitating for its expression the area gata factor. in contrast to what was observed for ot ...201020633690
flbc is a putative nuclear c2h2 transcription factor regulating development in aspergillus nidulans.asexual development (conidiation) in aspergillus is governed by multiple regulators. here, we characterize the upstream developmental activator flbc in aspergillus nidulans. flbc mrna is detectable throughout the life cycle, at relatively high levels during vegetative growth, early asexual and late sexual developmental phases. the deletion of flbc causes a delay/reduction in conidiation, brla and vosa expression, and conidial germination. while overexpression of flbc (oeflbc) does not elaborate ...201020624219
the extracellular β-1,3-endoglucanase enga is involved in autolysis of aspergillus elucidate the roles of the β-1,3-endoglucanase enga in autolysis of the filamentous fungus aspergillus nidulans and to identify the common regulatory elements of autolytic hydrolases.201020602653
aspergillus nidulans striatin (stra) mediates sexual development and localizes to the endoplasmic reticulum.striatin family proteins have been identified in animals and fungi and are considered to be scaffolding proteins. in fungi striatin orthologs have been associated with sexual development and virulence to plants. in this study, we characterized the functions and localization of the striatin ortholog, stra, in aspergillus nidulans. deltastra strains showed multiple defects in conidium germination, mycelial radial growth, production of diffusible red pigment, and reduced conidiation. the most strik ...201020601045
fitness-associated sexual reproduction in a filamentous is a long-standing evolutionary enigma. although the majority of eukaryotes reproduce sexually at least sometimes [1-3], the evolution of sex from an asexual ancestor has been difficult to explain because it requires sexually reproducing lineages to overcome the manifold costs of sex, including the destruction of favorable gene combinations created by selection [4, 5]. conditions for the evolution of sex are much broader if individuals can reproduce either sexually or asexually (i.e., facult ...201020598542
role of nek6 in tumor promoter-induced transformation in jb6 c141 mouse skin epidermal cells.nek6 (nima-related kinase 6) is a homologue of the aspergillus nidulans protein nima (never in mitosis, gene a). we demonstrate that overexpression of nek6 induces anchorage-independent transformation of jb6 cl41 mouse epidermal cells. tissue arrays and western immunoblot analysis show that nek6 is overexpressed in malignant tissues and several cancer cell lines. our data also show that nek6 interacts with stat3, an oncogenic transcription factor, and phosphorylates stat3 on ser(727), which is i ...201020595392
wild-type mic distributions and epidemiological cutoff values for the triazoles and six aspergillus spp. for the clsi broth microdilution method (m38-a2 document).clinical breakpoints have not been established for mold testing. wild-type (wt) mic distributions (organisms in a species/drug combination with no detectable acquired resistance mechanisms) were defined in order to establish epidemiologic cutoff values (ecvs) for five aspergillus spp. and itraconazole, posaconazole, and voriconazole. also, we have expanded prior ecv data for aspergillus fumigatus. the number of available isolates varied according to the species/triazole combination as follows: 1 ...201020592159
thermotolerance of germlings and mycelium of the insect-pathogenic fungus metarhizium spp. and mycelial recovery after heat stress.the upper temperature limits for germination and growth were determined for metarhizium acridum (arsef 324, 3341 and 3609), m. robertsii (arsef 2575), m. anisopliae (arsef 5749), and aspergillus nidulans (atcc 10074). most of the metarhizium species germinated well at 35 and 36 degrees c; however, compared to 28 degrees c, the growth was very slow (except arsef 5749 from mexico, which germinated at 35 degrees c, but did not grow at 34 or 36 degrees c). germination was severely impaired at 38 and ...201020586069
spotlight on aspergillus nidulans photosensory systems.aspergilli are ubiquitous soil-borne fungi growing within or on the surface of numerous organic substrates. growth within a substrate or growth on the surface correlates to different growth conditions for the hyphae due to significant changes in oxygen or reactive oxygen species levels and variations in humidity or temperature. the production of air-borne spores is supported by the substrate-air interphase and also requires a sensing system to adapt appropriately. here we focus on light as impor ...201020573560
identification of potato genes induced during colonization by phytophthora infestans.summary suppression subtractive hybridization (ssh) was applied in a search for genes induced during the compatible interaction between phytophthora infestans and potato. using potato leaves that had been treated with benzo(1,2,3)thiadiazole-7-carbothioic acid s-methylester (bth) as the control tissue, a low redundancy library with a relatively low frequency of the classic plant pathogenesis-related (pr) genes was generated. 288 of the clones were screened for induced sequences using inverse nor ...200120573000
ffvel1 and fflae1, components of a velvet-like complex in fusarium fujikuroi, affect differentiation, secondary metabolism and virulence.besides industrially produced gibberellins (gas), fusarium fujikuroi is able to produce additional secondary metabolites such as the pigments bikaverin and neurosporaxanthin and the mycotoxins fumonisins and fusarin c. the global regulation of these biosynthetic pathways is only poorly understood. recently, the velvet complex containing vea and several other regulatory proteins was shown to be involved in global regulation of secondary metabolism and differentiation in aspergillus nidulans. here ...201020572938
pathogenic fungi: leading or led by ambient ph?summary pathogenic fungi have successfully attacked a wide range of hosts, which has forced them into ambient-adaptation. ph is one of the major ambient traits affecting the activity of pathogenicity factors secreted by the pathogen, hence, a ph sensing-response system was developed to enable the pathogen to tailor its arsenal to best fit its host. the pacc pala, b, c, f, h and i apparatus was first identified in aspergillus nidulans and later found in other fungi. secreted pathogenicity factors ...200320569410
recombinant production of an aspergillus nidulans class i hydrophobin (dewa) in hypocrea jecorina (trichoderma reesei) is promoter-dependent.fungal hydrophobins have potential for several applications because of their abilities to change the hydrophobicity of different surfaces. yet because of their tendency for aggregation and attachment to interfacial areas only few production processes have so far been reported. towards the development of a heterologous production system, we report here the expression of a class i hydrophobin dewa of aspergillus nidulans in hypocrea jecorina (trichoderma reesei). using the h. jecorina hfb2 (class ...201020567818
the effect of resource quantity and resource stoichiometry on microbial carbon-use-efficiency.the carbon-use-efficiency (cue) of microorganisms is an important parameter in determining ecosystem-level carbon (c) cycling; however, little is known about how variance in resources affects microbial cue. to elucidate how resource quantity and resource stoichiometry affect microbial cue, we cultured four microorganisms - two fungi (aspergillus nidulans and trichoderma harzianum) and two bacteria (pectobacterium carotovorum and verrucomicrobium spinosum) - under 12 unique c, nitrogen (n) and ph ...201020550579
self-protection against gliotoxin--a component of the gliotoxin biosynthetic cluster, glit, completely protects aspergillus fumigatus against exogenous gliotoxin.gliotoxin, and other related molecules, are encoded by multi-gene clusters and biosynthesized by fungi using non-ribosomal biosynthetic mechanisms. almost universally described in terms of its toxicity towards mammalian cells, gliotoxin has come to be considered as a component of the virulence arsenal of aspergillus fumigatus. here we show that deletion of a single gene, glit, in the gliotoxin biosynthetic cluster of two a. fumigatus strains, rendered the organism highly sensitive to exogenous g ...201020548963
methylenetetrahydrofolate reductase activity is involved in the plasma membrane redox system required for pigment biosynthesis in filamentous fungi.methylenetetrahydrofolate reductases (mthfrs) play a key role in biosynthesis of methionine and s-adenosyl-l-methionine (sam) via the recharging methionine biosynthetic pathway. analysis of 32 complete fungal genomes showed that fungi were unique among eukaryotes by having two mthfrs, met12 and met13. the met12 type contained an additional conserved sequence motif compared to the sequences of met13 and mthfrs from other eukaryotes and bacteria. targeted gene replacement of either of the two mthf ...201020543064
antifungal activity of eugenol against penicillium, aspergillus, and fusarium species.the antifungal activity of eugenol in a model system against aspergilli (aspergillus niger, aspergillus terreus, and emericella nidulans), penicilli (penicillium expansum, penicillium glabrum, and penicillium italicum), and fusaria (fusarium oxysporum and fusarium avenaceum) was investigated. minimum detection time (time to attain a colony diameter of 1 cm) and the kinetic parameters were evaluated. the effectiveness of the active compound seemed to be strain or genus dependent; 100 mg/liter rep ...201020537272
aspergillus rabb rab5 integrates acquisition of degradative identity with the long distance movement of early endosomes.aspergillus nidulans early endosomes display characteristic long-distance bidirectional motility. simultaneous dual-channel acquisition showed that the two rab5 paralogues rabb and raba colocalize in these early endosomes and also in larger, immotile mature endosomes. however, rabb-gtp is the sole recruiter to endosomes of vps34 pi3k (phosphatidylinositol-3-kinase) and the phosphatidylinositol-3-phosphate [pi(3)p] effector anvps19 and rabb delta, leading to thermosensitivity prevents multivesicu ...201020534811
role of phospholipase c and protein kinase c in aspergillus nidulans during growth on pectin or glucose: effects on germination and duplication cycle.the effects of plc and pkc inhibitors on aspergillus nidulans depend on the carbon source. plc inhibitors spm and c48/80 delayed the first nuclear division in cultures growing on glucose, but stimulated it in media supplemented with pectin. less intense were these effects on the mutant transformed with plc-a gene rupture (ap27). neomycin also delayed the germination in cultures growing on glucose or pectin; however, on glucose, the nuclear division was inhibited whereas in pectin it was stimulat ...201020526834
the mode of reproduction in natural populations of ascomycetous fungus, emericella nidulans, from israel.the mode of reproduction of the soil ascomycetous fungus emericella nidulans of israeli populations was studied using 15 microsatellite (simple sequence repeats or ssr) trinucleotide markers. the study was performed in three canyons: two located in the northern part of israel (mount carmel and western upper galilee) and one in the southern negev desert. in each canyon, e. nidulans strains were isolated from the opposite slopes and (in the desert canyon) the valley bottom. testing the reproductiv ...201020515512
an activating mutation of the sclerotinia sclerotiorum pac1 gene increases oxalic acid production at low ph but decreases virulence.summary the production of oxalic acid by sclerotinia sclerotiorum is regulated by the ambient ph environment. this regulation and that of a few investigated ph-responsive genes is mediated in part by the zinc finger transcription factor encoded by pac1, an orthologue of the aspergillus nidulans pacc gene. we manipulated the pac1 sequence by site-directed mutagenesis to create a dominant activating pac1(c) mutation and introduced this allele into a pac1 loss-of-function (deltapac1) strain. consis ...200720507525
a defect in nir1, a nira-like transcription factor, confers morphological abnormalities and loss of pathogenicity in colletotrichum acutatum.summary a non-pathogenic mutant of colletotrichum acutatum, designated ca5, exhibited epiphytic hyphal growth and did not cause lesions on strawberry plants but grew necrotrophically when inoculated directly onto wounded stolons. in the absence of an external nitrogen source, the mutant exhibited extended germ-tube growth prior to appressorium formation. the deduced product of the impaired gene (nir1) is similar to nira, an aspergillus nidulans transcriptional regulator of nitrogen metabolism. i ...200620507451
the transcription repressor nmra is subject to proteolysis by three aspergillus nidulans proteases.the role of specific cleavage of transcription repressor proteins by proteases and how this may be related to the emerging theme of dinucleotides as cellular signaling molecules is poorly characterized. the transcription repressor nmra of aspergillus nidulans discriminates between oxidized and reduced dinucleotides, however, dinucleotide binding has no effect on its interaction with the zinc finger in the transcription activator area. protease activity in a. nidulans was assayed using nmra as th ...201020506376
nek1 silencing slows down dna repair and blocks dna damage-induced cell cycle arrest.never in mitosis a (nima)-related kinases (nek) are evolutionarily conserved proteins structurally related to the aspergillus nidulans mitotic regulator nima. nek1 is one of the 11 isoforms of the neks identified in mammals. different lines of evidence suggest the participation of nek1 in response to dna damage, which is also supported by the interaction of this kinase with proteins involved in dna repair pathways and cell cycle regulation. in this report, we show that cells with nek1 knockdown ...201020501547
gene silencing of transgenes inserted in the aspergillus nidulans alcm and/or alcs loci.while carrying out a systematic disruption of the genes of unknown function in the alc gene cluster from the filamentous fungus aspergillus nidulans, we observed a strong diminution of the transcription of markers inserted in the alcs gene. this was found to be the case for the two markers tested, nada (from a. nidulans) and pyrg (from a. fumigatus) involved in purine utilization and uracil/uridine biosynthetic pathway, respectively. the same phenomenon was also observed with insertion of the na ...201020495807
atp-citrate lyase is required for production of cytosolic acetyl coenzyme a and development in aspergillus nidulans.acetyl coenzyme a (coa) is a central metabolite in carbon and energy metabolism and in the biosynthesis of cellular molecules. a source of cytoplasmic acetyl-coa is essential for the production of fatty acids and sterols and for protein acetylation, including histone acetylation in the nucleus. in saccharomyces cerevisiae and candida albicans acetyl-coa is produced from acetate by cytoplasmic acetyl-coa synthetase, while in plants and animals acetyl-coa is derived from citrate via atp-citrate ly ...201020495057
the fps1p aquaglyceroporin facilitates the use of small aliphatic amides as a nitrogen source by amidase-expressing yeasts.saccharomyces cerevisiae acquires a resistance to high, toxic levels of acetic acid by destabilizing fps1p, the plasma membrane aquaglyceroporin through which this acid - in its undissociated state - enters the cell. in this study, fps1p loss was shown to confer resistances to acetic acid, acrolein and allyl alcohol, not just in s. cerevisiae but also in the osmotolerant spoilage yeast zygosaccharomyces rouxii. however, in z. rouxii, the loss of fps1p severely compromised the use of acetamide an ...201020491941
core promoters of the penicillin biosynthesis genes and quantitative rt-pcr analysis of these genes in high and low production strain of penicillium chrysogenum.the transcription start points of the penicillin biosynthesis genes from penicillium chrysogenum were mapped using the primer extension method. for each of the three genes consensus sequences of the core promoter elements were identified, supporting the notion that the basal transcription of these genes is mediated separately. interestingly, transcription start of the pcbc gene is located within the potential inr element with no tata box-like sequence being found at expected position. this is in ...201020490754
high-throughput screening of dna binding sites for transcription factor amyr from aspergillus nidulans using dna beads display system.we established a high-throughput screening method for the dna binding sequence of a eukaryotic transcription factor by using the bead display system with emulsion pcr and flow cytometry and applied it for identifying a eukaryotic transcriptional activator amyr, which is known to regulate amylolytic gene expression in aspergillus species. segmented parts of the binding site of amyr were randomized to make a dna library on beads, onto which male-tagged amyr protein and fluorescent anti-male tag an ...201020471587
development of rapid and specific molecular discrimination methods for pathogenic emericella species.aspergillosis is an important mycosis caused primarily by aspergillus fumigatus and its relatives. the genus emericella is a teleomorph related to the aspergillus section nidulantes. the typical anamorphic stage species in this genus is aspergillus nidulans, which is sometimes a significant agent in chronic granulomatous disease (cgd) patients. the mortality rate of osteomyelitis in cgd patients due to a. nidulans ( e. nidulans ) is very high compared to that due to a. fumigatus. moreover, two e ...201020467199
in vitro susceptibility testing in aspergillus species: an update.aspergillus species are the most common causes of invasive mold infections in immunocompromised patients. the introduction of new antifungal agents and recent reports of resistance emerging during treatment of aspergillus infections have highlighted the need for in vitro susceptibility testing. various testing procedures have been proposed, including macro- and micro-dilution, disk diffusion, etest (ab biodisk, sweden) and other commercial tests. although aspergillus species are generally suscep ...201020441550
analysis of a novel calcium auxotrophy in aspergillus aspergillus nidulans a combination of null mutations in hala, encoding a protein kinase, and slta, encoding a zinc-finger transcription factor having no yeast homologues, results in an elevated calcium requirement ('calcium auxotrophy') without impairing net calcium uptake. slta(-) (+/-hala(-)) mutations result in hypertrophy of the vacuolar system. in hala(-)slta(-) (and slta(-)) strains, transcript levels for pmca and pmcb, encoding vacuolar ca(2+)-atpase homologues, are highly elevated, su ...201020438880
predicting the chemical composition and structure of aspergillus nidulans hyphal wall surface by atomic force fungi, cell wall plays an important role in growth and development. major macromolecular constituents of the aspergilli cell wall are glucan, chitin, and protein. we examined the chemical composition and structure of the aspergillus nidulans hyphal wall surface by an atomic force microscope (afm). to determine the composition of the cell wall surface, the adhesion forces of commercially available beta-glucan, chitin, and various proteins were compared to those of corresponding fractions prepa ...201020437158
differential expression of cita gene encoding the mitochondrial citrate synthase of aspergillus nidulans in response to developmental status and carbon an extension of our previous studies on the mitochondrial citrate synthase of aspergillus nidulans and cloning of its coding gene (cita), we analyzed differential expression of cita in response to the progress of development and change of carbon source. the cdna consisted of 1,700 nucleotides and was predicted to encode a 474-amino acid protein. by comparing the cdna sequence with the corresponding genomic sequence, we confirmed that cita gene contains 7 introns and that its transcription sta ...201020437151
fungal secondary metabolites - strategies to activate silent gene clusters.filamentous fungi produce a multitude of low molecular weight bioactive compounds. the increasing number of fungal genome sequences impressively demonstrated that their biosynthetic potential is far from being exploited. in fungi, the genes required for the biosynthesis of a secondary metabolite are clustered. many of these bioinformatically newly discovered secondary metabolism gene clusters are silent under standard laboratory conditions. consequently, no product can be found. this review summ ...201120433937
[guideline based treatment of invasive aspergillosis].invasive fungus infections caused by aspergillus spp. occur most frequently in immunocompromised patients. a high infection-associated death rate of up to and over 50% is attributed even today to these fungi. the disease in humans is caused mainly by aspergillus fumigatus, aspergillus flavus and aspergillus niger. other species, for example, aspergillus terreus or aspergillus nidulans are quantitatively less prevalent. evidence based treatment of invasive aspergillosis has become safer and more ...201020433655
the choc gene encoding a putative phospholipid methyltransferase is essential for growth and development in aspergillus nidulans.phosphatidylcholines (pcs) are a class of major cell membrane phospholipids that participate in many physiological processes. three genes, choa, chob and choc, have been proposed to function in the endogenous biosynthesis of pc in aspergillus nidulans. in this study, we characterize the choc gene encoding a putative highly conserved phospholipid methyltransferase. the previously reported choc3 mutant allele results from a mutation leading to the e177k amino acid substitution. the transcript of c ...201020379720
receptor-independent ambient ph signaling by ubiquitin attachment to fungal arrestin-like palf.the seven-transmembrane receptor palh and its coupled, positive-acting arrestin-like protein palf are key components of a molecular sensor that in aspergillus nidulans and other ascomycete fungi mediates activation of an intracellular signaling cascade by alkaline ambient ph. palf is ubiquitinated in an alkaline ph- and palh-dependent manner. we show here that palf assists the plasma membrane localization of palh and that palf overexpression slightly hypersensitizes the pathway to alkaline ph bu ...201020368671
single-step affinity purification for fungal proteomics.a single-step protein affinity purification protocol using aspergillus nidulans is described. detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. examples defining the utility of the approaches, which should be widely applicable, are included.201020363899
characterization of aspergillus nidulans didb did2, a non-essential component of the multivesicular body pathway.escrt-iii heteropolymers mediate membrane protein cargo sorting into multivesicular endosomes for subsequent vacuolar degradation. we studied the localization of largely uncharacterized aspergillus nidulans escrt-iii using its key structural component vps32 and the 'associated' component didb(did2). vps32-gfp localizes to motile early endosomes as reported, but predominates in aggregates often associated with vacuoles due to inability to dissociate from endosomes. didb(did)(2) regulating vps4 (t ...201020362686
gdp-mannose transporter paralogues play distinct roles in polarized growth of aspergillus nidulans.gdp-mannose transporters (gmt) carry gdp-mannose nucleotide sugars from the cytosol across the golgi apparatus membrane for use as substrates in protein glycosylation in plants, animals and fungi. genomes of some fungal species, such as the yeast saccharomyces cerevisiae, contain only one gene encoding a gmt, while others, including aspergillus nidulans, contain two (gmta and gmtb). we previously showed that cell wall integrity and normal hyphal morphogenesis in a. nidulans depend upon the funct ...201020361498
a septin from the filamentous fungus a. nidulans induces atypical pseudohyphae in the budding yeast s. cerevisiae.septins, novel cytoskeletal proteins, form rings at the bases of emerging round buds in yeasts and at the bases of emerging elongated hyphal initials in filamentous fungi.201020360845
aspergillus nidulans contains six possible fatty acyl-coa synthetases with faab being the major synthetase for fatty acid degradation.aspergillus nidulans can use a variety of fatty acids as sole carbon and energy sources via its peroxisomal and mitochondrial beta-oxidation pathways. prior to channelling the fatty acids into beta-oxidation, they need to be activated to their acyl-coa derivates. analysis of the genome sequence identified a number of possible fatty acyl-coa synthetases (fata, fatb, fatc, fatd, faaa and faab). faab was found to be the major long-chain synthetase for fatty acid degradation. faab was shown to local ...201020354844
interaction of the aspergillus nidulans microtubule-organizing center (mtoc) component apsb with gamma-tubulin and evidence for a role of a subclass of peroxisomes in the formation of septal mtocs.peroxisomes are a diverse class of organelles involved in different physiological processes in eukaryotic cells. although proteins imported into peroxisomes carry a peroxisomal targeting sequence at the c terminus (pts1) or an alternative one close to the n terminus (pts2), the protein content of peroxisomes varies drastically. here we suggest a new class of peroxisomes involved in microtubule (mt) formation. eukaryotic cells assemble mts from distinct points in the cell. in the fungus aspergill ...201020348383
novel insights into the functional role of three protein arginine methyltransferases in aspergillus nidulans.protein arginine methylation has been implicated in different cellular processes including transcriptional regulation by the modification of histone proteins. here we demonstrate significant in vitro activities and multifaceted specificities of aspergillus protein arginine methyltransferases (prmts) and we provide evidence for a role of protein methylation in mechanisms of oxidative stress response. we have isolated all three aspergillus prmts from fungal extracts and could assign significant hi ...201020338257
penicillin production by the aspergillus nidulans group. 194720264543
some aspects of penicillin production by aspergillus nidulans. 194720264542
deletion of the ring-finger peroxin 2 gene in aspergillus nidulans does not affect meiotic development.peroxins are required for protein import into peroxisomes as well as for peroxisome biogenesis and proliferation. loss-of-function mutations in genes for the ring-finger peroxins pex2, pex10 and pex12 lead to a specific block in meiosis in the ascomycete podospora anserina. however, loss of protein import into peroxisomes does not result in this meiotic defect. therefore, it has been suggested that these peroxins have a specific function required for meiosis. to determine whether this role is co ...201020236327
distinct roles for caf1, ccr4, edc3 and cuta in the co-ordination of transcript deadenylation, decapping and p-body formation in aspergillus nidulans.transcript degradation is a key step in gene regulation. in eukaryotes, mrna decay is generally initiated by removal of the poly(a) tail mediated by the ccr4-caf1-not complex. deadenylated transcripts are then rapidly degraded, primarily via the decapping-dependent pathway. components of this pathway can be localized into highly dynamic cytoplasmic foci, the mrna processing (p)-bodies. we have undertaken confocal fluorescence microscopy to monitor p-bodies in aspergillus nidulans. as in other or ...201020233300
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