| methylosarcina fibrata gen. nov., sp. nov. and methylosarcina quisquiliarum sp.nov., novel type 1 methanotrophs. | two novel species of obligate methane-oxidizing bacteria, isolated from landfill soil, were characterized. both strains were unusual in that some members of the population grew in irregularly shaped, refractile cell packets that resembled sarcina-like clusters. electron microscopy revealed that the cell packets were covered with a slime layer and the cells contained many large granular inclusion bodies. the individual cells of each strain were sometimes motile and had differing morphologies. iso ... | 2001 | 11321107 |
| family- and genus-level 16s rrna-targeted oligonucleotide probes for ecological studies of methanotrophic bacteria. | methanotrophic bacteria play a major role in the global carbon cycle, degrade xenobiotic pollutants, and have the potential for a variety of biotechnological applications. to facilitate ecological studies of these important organisms, we developed a suite of oligonucleotide probes for quantitative analysis of methanotroph-specific 16s rrna from environmental samples. two probes target methanotrophs in the family methylocystaceae (type ii methanotrophs) as a group. no oligonucleotide signatures t ... | 2001 | 11571178 |
| quantitative detection of methanotrophs in soil by novel pmoa-targeted real-time pcr assays. | methane oxidation in soils is mostly accomplished by methanotrophic bacteria. little is known about the abundance of methanotrophs in soils, since quantification by cultivation and microscopic techniques is cumbersome. comparison of 16s ribosomal dna and pmoa (alpha subunit of the particulate methane monooxygenase) phylogenetic trees showed good correlation and revealed five distinct groups of methanotrophs within the alpha and gamma subclasses of proteobacteria: the methylococcus group, the met ... | 2003 | 12732507 |
| abundance, activity, and community structure of pelagic methane-oxidizing bacteria in temperate lakes. | the abundance and activity of methane-oxidizing bacteria (mob) in the water column were investigated in three lakes with different contents of nutrients and humic substances. the abundance of mob was determined by analysis of group-specific phospholipid fatty acids from type i and type ii mob, and in situ activity was measured with a 14ch4 transformation method. the fatty acid analyses indicated that type i mob most similar to species of methylomonas, methylomicrobium, and methylosarcina made a ... | 2005 | 16269705 |
| methylosarcina lacus sp. nov., a methanotroph from lake washington, seattle, usa, and emended description of the genus methylosarcina. | an obligately methanotrophic bacterial strain, lw14t, isolated from the sediment of lake washington, seattle, usa, is described taxonomically. the isolate is an aerobic, gram-negative, non-motile bacterium capable of growth on methane, and possesses type i intracytoplasmic membranes (i.e. it is a type i methanotroph). the strain possesses particulate methane monooxygenase (mmo) and has no soluble mmo. formaldehyde is assimilated via the ribulose monophosphate cycle. the isolate grows within a ph ... | 2005 | 16280494 |
| differential effects of nitrogenous fertilizers on methane-consuming microbes in rice field and forest soils. | the impact of environmental perturbation (e.g., nitrogenous fertilizers) on the dynamics of methane fluxes from soils and wetland systems is poorly understood. results of fertilizer studies are often contradictory, even within similar ecosystems. in the present study the hypothesis of whether these contradictory results may be explained by the composition of the methane-consuming microbial community and hence whether methanotrophic diversity affects methane fluxes was investigated. to this end, ... | 2006 | 16461686 |
| identification of active methanotrophs in a landfill cover soil through detection of expression of 16s rrna and functional genes. | active methanotrophs in a landfill soil were revealed by detecting the 16s rrna of methanotrophs and the mrna transcripts of key genes involved in methane oxidation. new 16s rrna primers targeting type i and type ii methanotrophs were designed and optimized for analysis by denaturing gradient gel electrophoresis. direct extraction of rna from soil enabled the analysis of the expression of the functional genes: mmox, pmoa and mxaf, which encode subunits of soluble methane monooxygenase, particula ... | 2007 | 17922768 |
| diversity of aerobic methanotrophic bacteria in a permafrost active layer soil of the lena delta, siberia. | with this study, we present first data on the diversity of aerobic methanotrophic bacteria (mob) in an arctic permafrost active layer soil of the lena delta, siberia. applying denaturing gradient gel electrophoresis and cloning of 16s ribosomal ribonucleic acid (rrna) and pmoa gene fragments of active layer samples, we found a general restriction of the methanotrophic diversity to sequences closely related to the genera methylobacter and methylosarcina, both type i mob. in contrast, we revealed ... | 2009 | 18592300 |
| stable isotope probing analysis of the diversity and activity of methanotrophic bacteria in soils from the canadian high arctic. | the melting of permafrost and its potential impact on ch(4) emissions are major concerns in the context of global warming. methanotrophic bacteria have the capacity to mitigate ch(4) emissions from melting permafrost. here, we used quantitative pcr (qpcr), stable isotope probing (sip) of dna, denaturing gradient gel electrophoresis (dgge) fingerprinting, and sequencing of the 16s rrna and pmoa genes to study the activity and diversity of methanotrophic bacteria in active-layer soils from ellesme ... | 2010 | 20622133 |
| seasonal change in methanotrophic diversity and populations in a rice field soil assessed by dna-stable isotope probing and quantitative real-time pcr. | the community structure of methane-oxidizing bacteria (methanotrophs) is affected by concentrations of methane and oxygen. in rice fields, concentrations of both gases differ significantly between the flooded and drained seasons. we investigated the active methanotrophic community structures in flooded and drained soils by dna-based stable isotope probing. active methanotrophic diversity was assessed with clone library-based analyses of the 16s rrna gene and the particulate methane monooxygenase ... | 2010 | 21576868 |
| earthworm activity in a simulated landfill cover soil shifts the community composition of active methanotrophs. | landfills represent a major source of methane in the atmosphere. in a previous study, we demonstrated that earthworm activity in landfill cover soil can increase soil methane oxidation capacity. in this study, a simulated landfill cover soil mesocosm (1 m × 0.15 m) was used to observe the influence of earthworms (eisenia veneta) on the active methanotroph community composition, by analyzing the expression of the pmoa gene, which is responsible for methane oxidation. mrna-based pmoa microarray an ... | 2011 | 21925596 |
| Structure and function of methanotrophic communities in a landfill-cover soil. | In landfill-cover soils aerobic methane-oxidizing bacteria (MOB) convert CH(4) to CO(2) , mitigating emissions of the greenhouse gas CH(4) to the atmosphere. We investigated overall MOB community structure and assessed spatial differences in MOB diversity, abundance and activity in a Swiss landfill-cover soil. Molecular cloning, terminal restriction-length polymorphism (T-RFLP) and quantitative PCR of pmoA genes was applied to soil collected from 16 locations at three different depths to study ... | 2011 | 22172054 |