reclassification of cellulomonas cellulans (stackebrandt and keddie 1986) as cellulosimicrobium cellulans gen. nov., comb. nov.phylogenetic analysis of 16s rdna provides evidence that cellulomonas cellulans branches outside the phylogenetic confines of the genus cellulomonas. the distinct phylogenetic and chemotaxonomic status of cellulomonas cellulans as a phylogenetic neighbour of the genus promicromonospora, justifies the description of a new genus for which the name cellulosimicrobium gen. nov. with the type species cellulosimicrobium cellulans comb. nov. is proposed.200111411667
cellulosimicrobium variabile sp. nov., a cellulolytic bacterium from the hindgut of the termite mastotermes darwiniensis.a novel cellulolytic and xylanolytic bacterium, strain mx5t, was isolated from the hindgut contents of the australian termite mastotermes darwiniensis (froggatt). the isolate was a facultative anaerobe and had a gram-positive cell-wall profile. the rod-shaped bacterium formed irregular coryneform and coccoid cells during growth. phylogenetic analysis of the 16s rdna provided evidence that the organism was closely related to the as-yet undescribed cellulolytic strain sr272 and the non-validly des ...200212148626
xylanimonas cellulosilytica gen. nov., sp. nov., a xylanolytic bacterium isolated from a decayed tree (ulmus nigra).a bacterial strain, designated xil07t, isolated from a decayed tree, ulmus nigra, in salamanca (spain) produced abundant cellulases and xylanases. the micro-organism was gram-positive, aerobic, coccoid and non-motile. growth was observed on many carbohydrates, including cellulose and xylan as the sole carbon sources. no growth was observed with acetate, citrate, gluconate, inositol, malate or mannitol as carbon sources. the strain showed very weak catalase activity. hplc analysis of menaquinones ...200312656159
effective extracellular trehalose production by cellulosimicrobium cellulans.a bacterium isolated from a petal of casa blanca lily (st26 strain) produced a marked amount of extracellular trehalose (alpha- d-glucopyranosyl-[1,1]-alpha- d-glucopyranose) in culture medium containing glucose. 16s rdna-based phylogeny showed that st26 belongs to, or is related to, cellulosimicrobium cellulans, a close relative of cellulomonas spp. various cellulomonas strains obtained from culture collections also showed extracellular trehalose productivity, suggesting that trehalose producti ...200414685787
reclassification of cellulosimicrobium variabile bakalidou et al. 2002 as isoptericola variabilis gen. nov., comb. already depicted in the original publication, the type strain of the species cellulosimicrobium variabile bakalidou et al. 2002, dsm 10177(t), does not cluster unambiguously with the type species, cellulosimicrobium cellulans dsm 43879(t), in phylogenetic analysis. strain dsm 10177(t) is moderately related to the recently described species xylanimonas cellulosilytica, promicromonospora pachnodae and xylanibacterium ulmi, forming a lineage that branches between c. cellulans and members of the ...200415143008
reclassification of promicromonospora pachnodae cazemier et al. 2004 as xylanimicrobium pachnodae gen. nov., comb. nov.the recently described facultatively anaerobic promicromonospora pachnodae is phylogenetically only moderately related to authentic members of promicromonospora. p. pachnodae is closely related to xylanibacterium ulmi and slightly less closely related to xylanimonas cellulosilytica and isoptericola variabilis (basonym cellulosimicrobium variabile). members of the different genera of promicromonosporaceae have similar chemotaxonomic properties; they share the same peptidoglycan type (a4alpha) and ...200415280318
equine abortion and premature birth associated with cellulosimicrobium cellulans infection.during the 2002 and 2003 foaling seasons, cellulosimicrobium (cellumonas) cellulans (formerly oerskovia xanthineolytica) was the principal microorganism isolated from fetal tissues or placentas from cases of equine abortion, premature birth, and term pregnancies. significant pathologic findings included chronic placentitis and pyogranulomatous pneumonia. in addition, microscopic and macroscopic alterations in the allantochorion from 4 of 7 cases of placentitis were similar to those caused by cro ...200415305747
a plate assay for simultaneous screening of polysaccharide- and protein-degrading develop a plate assay for simultaneous screening of polysaccharide-degrading and protein-degrading micro-organisms.200515644106
population profiles of a stable, commensalistic bacterial culture grown with toluene under sulphate-reducing conditions.most bacteria present in nature are not culturable in pure culture by means of classic cultivation methods (pace nr, 1997, science 276:734-740; amann ri et al., 1995, microbiol rev 59:143-169.). however, it was recently shown that most aerobic heterotrophic bacteria could grow only on artificial media when other micro-organisms are present (kaeberlein t et al., 2002, science 296:1127-1129). because the sulphate reducer desulfobacula toluolica dsm 7467 and a bacterium (strain mv1) identified as c ...200516003722
genotypic characterization of bacteria cultured from duck characterize the bacterial composition of mallard duck faeces and determine if novel bacterial species are present that could be utilized as potential indicators of avian faecal contamination.200516033461
molecular detection of cellulosimicrobium cellulans as the etiological agent of a chronic tongue ulcer in a human immunodeficiency virus-positive patient.ulcerations appeared on the tongue of a 48-year-old human immunodeficiency virus-positive man. histological findings of the biopsy specimen and the fact that the patient had resided in louisiana led us to suspect "american histoplasmosis". a new ulcer appeared while the patient was being treated with itraconazole, and the gene for 16s rrna of cellulosimicrobium cellulans was amplified. the lesions healed during treatment with oral penicillin and azithromycin.200516081997
revisiting the cellulosimicrobium cellulans yeast-lytic beta-1,3-glucanases toolbox: a review.cellulosimicrobium cellulans (also known with the synonyms cellulomonas cellulans, oerskovia xanthineolytica, and arthrobacter luteus) is an actinomycete that excretes yeast cell wall lytic enzyme complexes containing endo-beta-1,3-glucanases [ec and] as key constituents. three genes encoding endo-beta-1,3-glucanases from two c. cellulans strains have been cloned and characterised over the past years. the betaglii and betagliia genes from strain dsm 10297 (also known as o. xanth ...200616545129
characterization of clinical isolates previously identified as oerskovia turbata: proposal of cellulosimicrobium funkei sp. nov. and emended description of the genus cellulosimicrobium.taxonomic studies were performed on 13 clinical isolates (ten of which were epidemiologically related) that had been previously identified as oerskovia turbata. comparative phylogenetic analysis, based on 16s rrna gene sequences, indicated that the isolates are closely related to cellulosimicrobium cellulans with sequence similarity values ranging from 99.5 to 99.8 %. chemotaxonomic results (fatty acid profiles and menaquinones) supported the inclusion of these isolates in the genus cellulosimic ...200616585698
clearance of cellulosimicrobium cellulans bacteremia in a child without central venous catheter removal.cellulosimicrobium cellulans (formerly known as oerskovia xanthineolytica) rarely causes human infection. infections have been reported in immunocompromised hosts or in patients with foreign bodies, such as catheters, where treatment has generally involved removal of the foreign body. we report on a case in which the organism was isolated in multiple blood cultures from a 13-year-old male. after initial therapy failed, treatment with vancomycin and rifampin resulted in infection clearance withou ...200616825406
[polyphasic evidence for the transfer of promicromonospora yunnanensis to cellulosimicrobium cellulans].polyphasic taxonomic investigations of promicromonospora yunnanensis as4.1333 deposited in the china general microbiological culture collection center (cgmcc) indicated that strain as4.1333 was closely related to cellulosimicrobium cellulans dsm43879(t), the two organisms shared a 16s rrna gene similarity of 99.6% which correspond to 5 nt differences at 1423 positions. corresponding dna-dna reassociation value was 89.3%, significantly higher than 70% cut-off point recommended for the delineation ...200617037045
[mitigating the repress of cinnamic acid to cucumber growth by microbial strain].cucumber is one of the most important vegetable species. its continuous planting has become a common practice demand in many areas of china, but an obstacle from continuous planting made sustainable production of this crop to be prohibited. the self-toxic effect was considered as an important negative factor to continuous cropping cucumber. and cinnamic acid was found to be the main substance to cause self-toxic. strain ha8, which isolated from waste water estuary in zhuhai city and has been aut ...200617302157
diffusion characteristics and controlled release of bacterial fertilizers from modified calcium alginate indigenous cellulosimicrobium cellulans gs6 isolate able to solubilize insoluble phosphate complexes in soil is a potential bacterial fertilizer. enclosure of the phosphate-solubilizing bacterium (psb) in biodegradable capsules may protect the psb cells inoculated into soil and, in the meantime, enable the control of cell release that confers long-term fertilizing effects. in this study, calcium alginate (ca) was used as the core matrix to encapsulate cells of c. cellulans gs6. the cell-liber ...200817482812
cultivable bacterial diversity of alkaline lonar lake, india.aerobic, alkaliphilic bacteria were isolated and characterized from water and sediment samples collected in the winter season, january 2002 from alkaline lonar lake, india, having ph 10.5. the total number of microorganisms in the sediment and water samples was found to be 10(2)-10(6) cfu g(-1) and 10(2)-10(4) cfu ml(-1), respectively. one hundred and ninety-six strains were isolated using different enrichment media. to study the bacterial diversity of lonar lake and to select the bacterial stra ...200817604989
cellulosimicrobium terreum sp. nov., isolated from soil.a gram-positive, yellow-pigmented, non-motile and rod-shaped or coccoid bacterial strain, ds-61t, was isolated from soil from dokdo, korea, and its taxonomic position was investigated by using a polyphasic approach. the strain grew optimally at ph 6.5-7.5 and 25 degrees c in the presence of 1.0% (w/v) nacl. strain ds-61t had peptidoglycan of the type based on l-lys-l-thr-d-asp and contained galactose as the only whole-cell sugar. mk-9(h4) was the predominant menaquinone and anteiso-c15:0 and iso ...200717978207
biochemical characterization and sequence analysis of a xylanase produced by an exo-symbiotic bacterium of gryllotalpa orientalis, cellulosimicrobium sp. exo-symbiotic bacterium capable of hydrolyzing xylan was isolated from the gut of the mole cricket, gryllotalpa orientalis, and identified as cellulosimicrobium sp. hy-12. the xylanase (xyla( csphy-12)) of this organism bound tightly to both deae and mono q resins, and its molecular mass (m(r)) was about 39.0 kda. the highest xylanase activity was observed at ph 6.0 and 60 degrees c. the enzyme was greatly suppressed by ca(2+), cu(2+), co(2+), and fe(2+) ions but not by mg(2+) and mn(2+). alt ...200818027102
culture-independent nested pcr method reveals high diversity of actinobacteria associated with the marine sponges hymeniacidon perleve and sponge sp.a culture-independent nested polymerase chain reaction (pcr) technique was used to investigate the diversity of actinobacteria communities associated with the sponges hymeniacidon perleve and sponge sp. the phylogenetic affiliation of sponge-derived actinobacteria was then assessed by 16s rrna sequencing of cloned dna fragments. a total of 196 positive clones were screened by restriction fragment length polymorphism (rflp) analysis; 48 unique operational taxonomic units (otus) were selected for ...200818670903
pyogenic flexor tenosynovitis associated with cellulosimicrobium cellulans.cellulosimicrobium cellulans, formerly known as oerskovia xanthineolytica, is a rare human pathogen, often in association with a foreign body. a case of pyogenic flexor tenosynovitis associated with c. cellulans in an immunocompetent boy is described, underlining the importance of prompt surgical and microbiologic evaluation.200818832122
isolation of bacteria which produce yeast cell wall-lytic enzymes and their isolate bacteria capable of producing enzymes that lyse cell wall, we screened soil samples from rokko mountain where is situated in west japan. the isolated strains kh1, kh2 and kh3 that secrete yeast cell wall lytic enzymes are described herein. the activity of the enzymes, after isolation from culture supernatants, were examined. the lytic enzyme produced by strain kh3 was the most active in both cell wall degradation and beta-1,3 glucanase activity in comparison with commercially availabl ...200818839628
bacterial communities of tyre monofill sites: growth on tyre shreds and investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria.200919239530
cloning of a new xylanase gene from streptomyces sp. tn119 using a modified thermal asymmetric interlaced-pcr specific for gc-rich genes and biochemical characterization.a bacterial strain, streptomyces sp. tn119, was isolated from the gut of batocera horsfieldi larvae and showed xylanolytic activity. a degenerate primer set was designed based on the base usage of g and c in actinobacteria xylanase-coding sequences belonging to the glycosyl hydrolases family 10 (gh 10), and used to clone the partial xylanase gene from streptomyces sp. tn119. a modified thermal asymmetric interlaced (tail)-pcr specific for high-gc genes, named gc tail-pcr, was developed to obtain ...201019412576
bacterial community and nitrogen fixation in the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae).the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), colonizes all pines species within its native range throughout north and central america. recently, this species was accidentally introduced to china, where it has caused severe damage in pine forests. it belongs to a group of beetles that spend most of their lives between the tree bark and sapwood, where it feeds on phloem: a poor substrate with very low nutritional value of nitrogen and toxic proper ...200919543937
phenotypic and molecular identification of cellulosimicrobium cellulans isolated from antarctic snow.we report for the first time the isolation of cellulosimicrobium cellulans from antarctic snow. this strain demonstrated physiological traits that were markedly different from that of the mesophilic c. cellulans type strain dsm 43879(t). the dominant cell wall sugars in c. cellulans were glucose, galactose and mannitol whereas rhamnose was the only major sugar in the type strain. cellular fatty acid patterns were dominated by 12-methyltetradecanoic acid (ai-c(15:0)), hexadecanoic acid (c(16:0)) ...200919760124
novel gh10 xylanase, with a fibronectin type 3 domain, from cellulosimicrobium sp. strain hy-13, a bacterium in the gut of eisenia fetida.the gene encoding a novel modular xylanase from cellulosimicrobium sp. strain hy-13 was identified and expressed in escherichia coli, and its truncated gene product was characterized. the enzyme consisted of three distinct functional domains, an n-terminal catalytic gh10 domain, a fibronectin type 3 domain, and c-terminal carbohydrate-binding module 2.200919767466
[phylogenetic diversity of the culturable rare actinomycetes in marine sponge hymeniacidon perlevis by improved isolation media].based on the molecular diversity information, seven actinomycete-selective culture media and isolation conditions were modified to isolate and cultivate diverse rare actinomycetes from hymeniacidon perlevis.200919873748
quantification and identification of culturable airborne bacteria from duck houses.employees at agricultural working places are often exposed to complex bioaerosols. investigations of bioaerosols in duck houses revealed concentrations of cultivable bacteria between 0.4 and 3 x 10(5) colony forming units (cfu) m(-3) on tryptone soy agar, 0.3 and 2 x 10(5) cfu m(-3) on actinomycetes isolation agar, and 0.8 and 5 x 10(3) cfu m(-3) on middlebrook agar, respectively, when incubated at 25 degrees c. at an incubation temperature of 37 degrees c, 0.6-3 x 10(2) cfu m(-3) were counted o ...201020042465
early-onset neonatal sepsis due to cellulosimicrobium cellulans.cellulosimicrobium cellulans represents a rare human pathogen. infections have been reported in immunocompromised hosts or in patients with an underlying disease. the authors describe a rare case of early-onset neonatal sepsis due to cellulosimicrobium cellulans in an infant without any underlying disease. the infant was successfully treated with vancomycin.201020376528
a cluster of acute-onset postoperative endophthalmitis over a 1-month period: investigation of an outbreak caused by uncommon report the clinical course, treatment response and prognosis of eight cases which developed acute-onset postoperative endophthalmitis over a 1-month period.201120733020
cellulosimicrobium funkei: first report of infection in a nonimmunocompromised patient and useful phenotypic tests for differentiation from cellulosimicrobium cellulans and cellulosimicrobium terreum.cellulosimicrobium funkei is a rare, opportunistic pathogen. we describe a case of bacteremia and possibly prosthetic valve endocarditis by this organism in a nonimmunocompromised patient. useful phenotypic tests for differentiating c. funkei from cellulosimicrobium cellulans and cellulosimicrobium terreum include motility, raffinose fermentation, glycogen, d-xylose, and methyl-α-d-glucopyranoside assimilation, and growth at 35°c.201121227990
[first case of peritoneal infection due to oerskovia turbata (cellulosimicrobium funkei)]. 201121461020
cloning and characterization of a modular gh5 ß-1,4-mannanase with high specific activity from the fibrolytic bacterium cellulosimicrobium sp. strain hy-13.the gene (1272-bp) encoding a ß-1,4-mannanase from a gut bacterium of eisenia fetida, cellulosimicrobium sp. strain hy-13 was cloned and expressed in escherichia coli. the recombinant ß-1,4-mannanase (rmanh) was approximately 44.0kda and has a catalytic gh5 domain that is 65% identical to that of the micromonospora sp. ß-1,4-mannosidase. the enzyme exhibited the highest catalytic activity toward mannans at 50°c and ph 6.0. rmanh displayed a high specific activity of 14,711 and 8498iumg(-1) towar ...201121767948
septic arthritis due to cellulosimicrobium cellulans.cellulosimicrobium cellulans has been reported as a rare cause of human pathogenesis. infections mainly occur in immunocompromised patients and very often are associated with a foreign body. we report the first case of septic arthritis caused by c. cellulans in an immunocompetent patient. our patient suffered a penetrating palm tree thorn injury to his left knee 8 weeks before admission. although no foreign objects were found, they were suspected because previous reports suggest a frequent assoc ...201121998421
Critical roles of Asp270 and Trp273 in the a-repeat of the carbohydrate-binding module of endo-1,3-ß-glucanase for laminarin-binding avidity.A carbohydrate-binding module from family 13 (CBM13), appended to the catalytic domain of endo-1,3-ß-glucanase from Cellulosimicrobium cellulans, was overexpressed in E. coli, and its interactions with ß-glucans, laminarin and laminarioligosaccharides, were analyzed using surface plasmon resonance biosensor and isothermal titration calorimetry. The association constants for laminarin and laminarioligosaccharides were determined to be approximately 10(6) M(-1) and 10(4) M(-1), respectively, indic ...201122198269
A highly active endo-ß-1,4-mannanase produced by Cellulosimicrobium sp. strain HY-13, a hemicellulolytic bacterium in the gut of Eisenia fetida.A xylanolytic gut bacterium isolated from Eisenia fetida, Cellulosimicrobium sp. strain HY-13, produced an extracellular glycoside hydrolase capable of efficiently degrading mannose-based substrates such as locust bean gum, guar gum, mannotetraose, and mannopentaose. The purified mannan-degrading enzyme (ManK, 34,926Da) from strain HY-13 was found to have an N-terminal amino acid sequence of DEATTDGLHVVDD, which has not yet been identified. Under the optimized reaction conditions of 50°C and pH ...201122112951
Molecular characterization of endo-1,3-ß-glucanase from Cellulosimicrobium cellulans: Effects of carbohydrate-binding module on enzymatic function and stability.An endo-1,3-ß-glucanase was purified from Tunicase®, a crude enzyme preparation from Cellulosimicrobium cellulans DK-1, and determined to be a 383-residue protein (Ala1-Leu383), comprising a catalytic domain of the glycoside hydrolase family 16 and a C-terminal carbohydrate-binding module family 13. The Escherichia coli expression system of the catalytic domain (Ala1-Thr256) was constructed, and the protein with N-terminal polyhistidine tag was purified using a Ni-nitrilotriacetic acid column. W ...201121979581
microbial preference for different size classes of organic carbon: a study from antarctic snow.significance of carbon cycling in polar ecosystems is well recognized. yet, bacteria in surface snow have received less attention in terms of their potential in carbon cycling. here, we present results on carbon utilization by bacterial communities in three surface snow samples from antarctica collected along a coastal to inland transect. microcosm studies were conducted over 8 days at 5 ± 1°c to study carbon metabolism in different combinations of added low molecular weight (lmw (glucose, <1 kd ...201122037862
culture-based and denaturing gradient gel electrophoresis analysis of the bacterial community structure from the intestinal tracts of earthworms(eisenia fetida).the bacterial communities in the intestinal tracts of earthworm were investigated by culture-dependent and - independent approaches. in total, 72 and 55 pure cultures were isolated from the intestinal tracts of earthworms under aerobic and anaerobic conditions, respectively. aerobic bacteria were classified as aeromonas (40%), bacillus (37%), photobacterium (10%), pseudomonas (7%), and shewanella (6%). anaerobic bacteria were classified as aeromonas (52%), bacillus (27%), shewanella (12%), paeni ...201121952364
novel modular endo-β-1,4-xylanase with transglycosylation activity from cellulosimicrobium sp. strain hy-13 that is homologous to inverting gh family 6 enzymes.the gene (2304-bp) encoding a novel xylanolytic enzyme (xylk2) with a catalytic domain, which is 70% identical to that of cellulomonas flavigena dsm 20109 gh6 β-1,4-cellobiohydrolase, was identified from an earthworm (eisenia fetida)-symbiotic bacterium, cellulosimicrobium sp. strain hy-13. the enzyme consisted of an n-terminal catalytic gh6-like domain, a fibronectin type 3 (fn3) domain, and a c-terminal carbohydrate-binding module 2 (cbm 2). xylk2δfn3-cbm 2 displayed high transferase activity ...201122230776
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