Publications

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genetic diversity across a vertebrate species' range: a test of the central-peripheral hypothesis.although it has been long presumed that population genetic variability should decrease as a species' range margin is approached, results of empirical investigations remain ambiguous. sampling strategies employed by many of these studies have not adequately sampled the entire range. here we present the results of an investigation of population genetic diversity in a vertebrate species, the italian agile frog, rana latastei, sampled comprehensively across its entire range. our results show that ge ...200415078443
response of the italian agile frog (rana latastei) to a ranavirus, frog virus 3: a model for viral emergence in naïve populations.ranavirus (family iridoviridae) is a genus of pathogens of poikilotherms, and some ranaviruses may play a role in widespread mortality of amphibians. ecology of viral transmission in amphibians is poorly known but can be addressed through experimentation in the laboratory. in this study, we use the ranavirus frog virus 3 (fv3) as an experimental model for pathogen emergence in naive populations of tadpoles. we simulated emerging disease by exposing tadpoles of the italian agile frog (rana latast ...200415650083
genetic diversity, but not hatching success, is jointly affected by postglacial colonization and isolation in the threatened frog, rana latastei.both postglacial colonization and habitat fragmentation can reduce the genetic diversity of populations, which in turn can affect fitness. however, since these processes occur at different spatial and temporal scales, the consequences of either process may differ. to disentangle the relative role of isolation and postglacial colonization in determining genetic diversity and fitness, we studied microsatellite diversity of 295 individuals from 10 populations and measured the hatch rate of 218 clut ...200717444892
climatic oscillations triggered post-messinian speciation of western palearctic brown frogs (amphibia, ranidae).oscillating glacial cycles over the past 2.4 million years are proposed to have had a major impact on the diversity of contemporary species communities. we used mitochondrial and nuclear dna sequence data to infer phylogenetic relationships within western palearctic brown frogs and to test the influence of pliocene and pleistocene climatic changes on their evolution. we sequenced 1976bp of the mitochondrial genes 16s rrna and cytochrome b and of the nuclear rhodopsin gene for all current species ...200312565039
ltq orbitrap velos in routine de novo sequencing of non-tryptic skin peptides from the frog rana latastei with traditional and reliable manual spectra interpretation.mass spectrometry has shown itself to be the most efficient tool for the sequencing of peptides. however, de novo sequencing of novel natural peptides is significantly more challenging in comparison with the same procedure applied for the tryptic peptides. to reach the goal in this case it is essential to select the most efficient methods of triggering fragmentation and combine all the possible complementary techniques.201627071218
rapid selection against inbreeding in a wild population of a rare frog.populations that are small and isolated can be threatened through loss of fitness due to inbreeding. nevertheless, an increased frequency of recessive homozygotes could increase the efficiency of selection against deleterious mutants, thus reducing inbreeding depression. in wild populations, observations of evolutionary changes determined by selection against inbreeding are few. we used microsatellite dna markers to compare the genetic features of tadpoles immediately after hatch with those of m ...201125567951
a skeletochronological study of growth, longevity, and age at sexual maturity in a population of rana latastei (amphibia, anura).longevity and age at sexual maturity in an italian population of rana latastei were studied by skeletochronology performed on the phalanges. frogs collected in 1998 and 1999 by drift fences and pitfall traps were marked by toe-clipping. after marking, individuals were released and the cut phalanges were processed for skeletochronological analysis. the maximum age so far recorded was 3 years in males and 4 years in females. the smallest male and female that were sexually mature on the basis of hi ...200314660877
[cytospectrophotometric studies of erythrocytes of rana latastei during metamorphosis and in the adult]. 19715004505
[on the sialopolysaccharide component of ovular involucra of rana latastei on bufo vulgaris. preliminary research]. 19645896187
invasive crayfish does not influence spawning microhabitat selection of brown frogs.microhabitat selection is a key component of amphibian breeding biology and can be modulated in response to the features of breeding sites and the presence of predators. despite invasive alien species being among the major threats to amphibians, there is limited information on the role of invasive species in shaping amphibians' breeding microhabitat choice. the invasive red swamp crayfish (procambarus clarkii) is a major predator of amphibians' larvae, including those of the brown frogs rana dal ...202032328354
peptidomic analysis of the host-defense peptides in skin secretions of rana graeca provides insight into phylogenetic relationships among eurasian rana species.peptidomic analysis of norepinephrine-stimulated skin secretions from the greek stream frog rana graeca boulenger, 1891 led to the identification and structural characterization of a range of host-defense peptides. these comprised brevinin-1gra, brevinin-1grb and an n-terminally extended form of brevinin-1grb, ranatuerin-2gr together with its oxidized form and (11-28) fragment, temporin-gra, temporin-grb and its non-amidated form, and a melittin-related peptide, mrp-gr and its (1-18) fragment. t ...201930599276
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