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streptococcus infantarius sp. nov., streptococcus infantarius subsp. infantarius subsp. nov. and streptococcus infantarius subsp. coli subsp. nov., isolated from humans and food.eighteen strains isolated from human specimens or from food products were characterized as atypical variants of mannitol-negative streptococcus bovis. they were tested for extended biochemical criteria, ribotyping and dna-dna hybridization in order to define their taxonomic status. these strains were demonstrated to constitute a dna relatedness group that includes strains of dna group 4 of farrow et al. (1984). comparative analysis of 16s rrna sequences demonstrated that these strains represent ...200010939646
molecular monitoring and characterization of the faecal microbiota of healthy dogs during fructan supplementation.the large intestine of dogs contains a complex microbial ecosystem with predominance of streptococci, bifidobacteria, lactobacilli, bacteroides and clostridium. generally, this predominant microbiota in dogs is relatively stable in time but much less is known about its taxonomic composition. moreover, almost no studies have been conducted to investigate this stability of the faecal microbial population in dogs upon prebiotic administration. the objective of the present study was to monitor possi ...200515979820
transferable vanb2 tn5382-containing elements in fecal streptococcal strains from veal calves.three vancomycin-resistant veal calf fecal streptococci, identified as streptococcus gallolyticus (n = 2) and streptococcus lutetiensis, were shown to harbor vanb2 tn5382-like elements earlier described in enterococci. one s. gallolyticus strain had a 1,495-bp is256-related element inserted in vans(b). the vanb2 tn5382 element present in the plasmid-free s. lutetiensis strain was transferable to enterococcus faecium bm4105-rf, enterococcus faecalis jh2-2, and its recombination-deficient derivati ...200312878522
taxonomic dissection of the streptococcus bovis group by analysis of manganese-dependent superoxide dismutase gene (soda) sequences: reclassification of 'streptococcus infantarius subsp. coli' as streptococcus lutetiensis sp. nov. and of streptococcus bovis biotype 11.2 as streptococcus pasteurianus sp. nov.the taxonomic dissection of the streptococcus bovis-streptococcus equinus group was carried out upon obtaining sequences for the manganese-dependent superoxide dismutase gene (soda) of the type strains of s. bovis, streptococcus caprinus, s. equinus, streptococcus gallolyticus, streptococcus infantarius, streptococcus macedonicus and streptococcus waius. the soda sequences of 29 streptococcal strains of animal and human origin that were related to s. bovis were also sequenced. a phylogenetic ana ...200212148636
re-identification of streptococcus bovis isolates causing bacteraemia according with the new taxonomy criteria: still an issue?all streptococcus bovis blood culture isolates recovered from jan-2003 to jan-2010 (n=52) in our institution were re-identified using new taxonomic criteria based upon their genetic traits. initial identification was performed by the semiautomatic wider system (fco. soria-melguizo, spain) and the api 20 strep (biomérieux, france). all isolates were re-identified by pcr amplification and sequencing of both 16s rdna and soda genes and by mass spectrometry using maldi-tof ms (bruker, germany). resu ...201121752968
microbial ecology of the equine hindgut during oligofructose-induced laminitis.alimentary carbohydrate overload is a significant cause of laminitis in horses and is correlated with drastic shifts in the composition of hindgut microbiota. equine hindgut streptococcal species (ehss), predominantly streptococcus lutetiensis, have been shown to be the most common microorganisms culturable from the equine caecum prior to the onset of laminitis. however, the inherent biases of culture-based methods are estimated to preclude up to 70% of the normal caecal microbiota. the objectiv ...200818580970
comprehensive study of strains previously designated streptococcus bovis consecutively isolated from human blood cultures and emended description of streptococcus gallolyticus and streptococcus infantarius subsp. coli.modern taxonomy has delineated streptococcus gallolyticus subsp. gallolyticus, s. gallolyticus subsp. pasteurianus, streptococcus infantarius subsp. coli, and s. infantarius subsp. infantarius within the heterogenous group of previously designated clinical streptococcus bovis bacteria. in the present study, 58 consecutive blood culture isolates initially designated s. bovis were further characterized by applying phenotypic and molecular genetic methods, and possible disease associations were inv ...200818614655
analysis of bacterial communities of traditional fermented west african cereal foods using culture independent methods.in this study, the microbial composition of kunu-zaki and ogi, two popular foods in nigeria produced after natural, uncontrolled fermentation of cereals, was assessed by culture-independent molecular profiling methods. in particular, pcr-denaturing gradient gel electrophoresis and construction of 16s rrna gene clone libraries revealed the presence of diverse bacterial communities. dna sequencing of the highly variable v3 region of the 16s rrna genes obtained from pcr-dgge fingerprints identified ...201121272950
genotypic characterization of streptococcus infantarius subsp. coli isolates from sea otters with infective endocarditis and/or septicemia and from environmental mussel samples.pulsed-field gel electrophoresis (pfge) was used to type 128 streptococcus infantarius subsp. coli isolates from sea otters and mussels. six smai pfge groups were detected, with one predominant group representing 57% of the isolates collected over a wide geographic region. several sea otter and mussel isolates were highly related, suggesting that an environmental infection source is possible.201223052307
evaluation of bacterial flora during the ripening of kedong sufu, a typical chinese traditional bacteria-fermented soybean product.kedong sufu is a typical bacteria-fermented sufu in china. isolation and identification of the autochthonous bacteria involved would allow the design of specific starters for this speciality. the purpose of the present study was to evaluate the bacterial flora during the ripening of kedong sufu using polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge) and culturing.201323400969
dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated streptococcus lutetiensis.the sequences of the 16s rrna genes extracted from fecal samples provide insights into the dynamics of fecal microflora. this potentially gives valuable etiological information for patients whose conditions have been ascribed to unknown pathogens, which cannot be accomplished using routine culture methods. we studied 33 children with diarrhea who were admitted to the children's hospital in shanxi province during 2006.201323782707
bartonella spp. exposure in northern and southern sea otters in alaska and california.since 2002, an increased number of northern sea otters (enhydra lutris kenyoni) from southcentral alaska have been reported to be dying due to endocarditis and/or septicemia with infection by streptococcus infantarius subsp. coli. bartonella spp. dna was also detected in northern sea otters as part of mortality investigations during this unusual mortality event (ume) in kachemak bay, alaska. to evaluate the extent of exposure to bartonella spp. in sea otters, sera collected from necropsied and l ...201425514118
icesluvan, a 94-kilobase mosaic integrative conjugative element conferring interspecies transfer of vanb-type glycopeptide resistance, a novel bacitracin resistance locus, and a toxin-antitoxin stabilization system.a 94-kb integrative conjugative element (icesluvan) transferable to enterococcus faecium and enterococcus faecalis from an animal isolate of streptococcus lutetiensis consists of a mosaic of genetic fragments from different gram-positive bacteria. a variant of icesluvan was confirmed in s. lutetiensis from a patient. a complete tn5382/tn1549 with a vanb2 operon is integrated into a streptococcal icesde3396-like region harboring a putative bacteriophage exclusion system, a putative agglutinin rec ...201324078615
isolation and characterization of mimosine, 3, 4 dhp and 2, 3 dhp degrading bacteria from a commercial rumen inoculum.the presence of the toxic amino acid mimosine in leucaena leucocephala restricts its use as a protein source for ruminants. rumen bacteria degrade mimosine to 3,4- and 2,3-dihydroxypyridine (dhp), which remain toxic. synergistes jonesii is believed to be the main bacterium responsible for degradation of these toxic compounds but other bacteria may also be involved. in this study, a commercial inoculum provided by the queensland's department of agriculture, fisheries, and forestry was screened fo ...201626773324
effect of phytogenic feed additives in soybean meal on in vitro swine fermentation for odor reduction and bacterial community comparison.the effect of different phytogenic feed additives on reducing odorous compounds in swine was investigated using in vitro fermentation and analyzed their microbial communities. soybean meal (1%) added with 0.1% different phytogenic feed additives (fa) were in vitro fermented using swine fecal slurries and anaerobically incubated for 12 and 24 h. the phytogenic fas used were red ginseng barn powder (panax ginseng c. a. meyer, fa1), persimmon leaf powder (diospyros virginiana l., fa2), ginkgo leaf ...201325049786
[streptococcus bovis, taxonomic status, clinical relevance and antimicrobial susceptibility].streptococcus bovis is a large bacterial complex of facultative anaerobic gram-positive cocci that includes distinct, genetically-related species. traditionally, s. bovis was classified into three biotypes: i (mannitol fermentation-positive), ii/1 (mannitol-negative and β-glucuronidase-negative), and ii/2 (mannitol-negative and β-glucuronidase-positive). the introduction of molecular techniques in the last few decades has led to proposals for a genetic classification of this complex: s. bovis bi ...201323453226
novel bartonella infection in northern and southern sea otters (enhydra lutris kenyoni and enhydra lutris nereis).since 2002, vegetative valvular endocarditis (vve), septicemia and meningoencephalitis have contributed to an unusual mortality event (ume) of northern sea otters in southcentral alaska. streptococcal organisms were commonly isolated from vegetative lesions and organs from these sea otters. bartonella infection has also been associated with bacteremia and vve in terrestrial mammals, but little is known regarding its pathogenic significance in marine mammals. our study evaluated whether streptoco ...201424629902
streptococcus bovis/s. equinus complex septicemia in a group of calves following intramuscular vaccination.organisms previously classified as streptococcus bovis (i.e., the s. bovis/s. equinus complex) are common in cattle feces, but may also act as opportunistic pathogens. in the current work, streptococcus infantarius subsp. coli, a member of this complex, was associated of a cluster of calves that died within hours of injection with a modified live viral vaccine. within 12 h of vaccination of 46 calves at a cow/calf operation, 4 calves had died, 3 calves were ill, and 1 unvaccinated cow was dead. ...201627216720
novel real-time pcr assays using taqman minor groove binder probes for identification of fecal carriage of streptococcus bovis/streptococcus equinus complex from rectal swab specimens.real-time pcr based on the recn and gyrb genes was developed to detect four streptococcus bovis/streptococcus equinus complex (sbec) subspecies from rectal swab specimens. the overall prevalence was 35.2%: streptococcus gallolyticus subsp. gallolyticus (11.1%), s. gallolyticus subsp. pasteurianus (13%), streptococcus infantarius subsp. coli (20.4%), and s. infantarius subsp. infantarius (11.1%). to conclude, these real-time pcr assays provide a reliable molecular method to detect sbec pathogenic ...201424391203
first case of streptococcus lutetiensis bacteremia involving a clindamycin-resistant isolate carrying the lnub gene.here, we describe the first case of a streptococcus lutetiensis isolate harboring the lnub gene.201324048528
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