| nitroimidazole resistance genes (nimb) in anaerobic gram-positive cocci (previously peptostreptococcus spp.). | to investigate metronidazole resistance and the prevalence of nitroimidazole (nim) genes in clinically isolated anaerobic gram-positive cocci. | 2004 | 15150170 |
| serum bactericidal activities of moxifloxacin and levofloxacin against aerobic and anaerobic intra-abdominal pathogens. | we studied the serum bactericidal activity (sba) of moxifloxacin and levofloxacin against common pathogens associated with complicated intra-abdominal infections. ten healthy volunteers received a single dose of moxifloxacin (400 mg) and levofloxacin (750 mg) and serum samples were collected at 2, 4, 8, 12, and 24h after the dose of each drug. bactericidal titers in serum over time were determined for aerobic gram-negative bacilli (escherichia coli, klebseilla pneumoniae, and enterobacter cloaca ... | 2008 | 17983770 |
| development of 16s rrna-based probes for the identification of gram-positive anaerobic cocci isolated from human clinical specimens. | fluorescent probes targeted at 16s rrna were designed for peptostreptococcus anaerobius and peptostreptococcus stomatis (pana134), parvimonas micra (pamic1435), finegoldia magna (fmag1250), peptoniphilus asaccharolyticus (pnasa1254), peptoniphilus ivorii (pnivo731), peptoniphilus harei (pnhar1466), anaerococcus vaginalis (avag1280) and anaerococcus lactolyticus (alac1438), based on the 16s rrna sequences of reference strains and 88 randomly chosen clinical isolates. these strains were also used ... | 2007 | 17714522 |
| [nonpuerperal breast abscess caused by finegoldia magna]. | finegoldia magna is a species of strictly anaerobic gram-positive cocci, arranged in pairs, tetrads, and clusters. these organisms are components of the normal flora of the skin, gastrointestinal and genitourinary female tracts, and oral cavity. they are asaccharolytic and their major energy sources are aminoacids and peptones. the species is usually isolated in polymicrobial cultures from abscesses, soft tissue infections, bone and joints. in the case herein presented, f. magna was recovered in ... | 2007 | 17702255 |
| crystal structure of a bacterial albumin-binding domain at 1.4 a resolution. | the albumin-binding domain, or ga module, of the peptostreptococcal albumin-binding protein expressed in pathogenic strains of finegoldia magna is believed to be responsible for the virulence and increased growth rate of these strains. here we present the 1.4a crystal structure of this domain, and compare it with the crystal structure of the ga-albumin complex. an analysis of protein-protein interactions in the two crystals, and the presence of multimeric ga species in solution, indicate the ga ... | 2007 | 17575979 |
| human serum activity of telithromycin, azithromycin and amoxicillin/clavulanate against common aerobic and anaerobic respiratory pathogens. | telithromycin is a new ketolide antimicrobial with a good in vitro activity against both aerobic and anaerobic respiratory pathogens. in this study, we evaluated the antibacterial activity over time of telithromycin (800mg), azithromycin (500mg), and amoxicillin/clavulanate (875/125mg) in serum following single oral doses of these agents to 10 healthy subjects. inhibitory and bactericidal titers were determined at 2, 6, 12, and 24h after each dose and the median titer was used to determine antib ... | 2007 | 17189093 |
| sufa--a novel subtilisin-like serine proteinase of finegoldia magna. | finegoldia magna is an anaerobic gram-positive bacterium and commensal, which is also associated with clinically important conditions such as skin and soft tissue infections. this study describes a novel subtilisin-like extracellular serine proteinase of f. magna, denoted sufa (subtilase of finegoldia magna), which is believed to be the first subtilase described among gram-positive anaerobic cocci. sufa is associated with the bacterial cell surface, but is also released in substantial amounts du ... | 2007 | 18048934 |
| in vitro activities of dalbavancin and 12 other agents against 329 aerobic and anaerobic gram-positive isolates recovered from diabetic foot infections. | tests of dalbavancin's in vitro activity against 209 aerobic and 120 anaerobic isolates from pretreatment diabetic foot infections showed an mic(90) of < or =0.125 microg/ml against methicillin-susceptible staphylococcus aureus (mssa), methicillin-resistant s. aureus (mrsa), and 120 anaerobes (clostridium perfringens, other clostridia, peptoniphilus asaccharolyticus, finegoldia magna, and anaerococcus prevotii), compared to respective mic(90)s for mssa and mrsa of 0.5 and 1 microg/ml for vancomy ... | 2006 | 16870792 |
| in vitro activities of daptomycin, vancomycin, and penicillin against clostridium difficile, c. perfringens, finegoldia magna, and propionibacterium acnes. | daptomycin has in vitro activity against gram-positive anaerobic bacteria, although limited numbers of species have been tested. we studied the in vitro activities of daptomycin, vancomycin, and penicillin against more than 100 strains each of clostridium difficile, c. perfringens, finegoldia magna, and propionibacterium acnes. daptomycin etest mics and results from time-kill studies were determined for selected strains. for 392 of 421 strains (93%), daptomycin was inhibitory at < or =1 microg/m ... | 2006 | 16870765 |
| in vitro activity of oritavancin (ly333328), vancomycin, clindamycin, and metronidazole against clostridium perfringens, propionibacterium acnes, and anaerobic gram-positive cocci. | using an agar dilution method, we determined the in vitro activity of oritavancin, vancomycin, clindamycin and metronidazole against 114 unique clinical isolates of gram-positive anaerobes. mic(90)s (microg/ml) for oritavancin were as follows: clostridium perfringens 1.0, propionibacterium acnes 0.25, peptostreptococcus anaerobius 0.25, peptoniphilus asaccharolyticus 0.5, finegoldia magna 0.25, micromonas. micros 0.25, and anaerococcus prevotii 0.25. on a weight basis, oritavancin is slightly mo ... | 2005 | 16701537 |
| anaerobic microbiology in 198 cases of pleural empyema: a bulgarian study. | the aim of the study was to evaluate the incidence of anaerobic bacteria in 198 patients with pleural empyema and the susceptibility of isolates to eight antibacterial agents. isolates were identified by the crystal anaerobes identification system, api system rapid id 32 a and/or routine methods. susceptibility was tested by sceptor mic system for anaerobic bacteria and limited agar dilution method. anaerobic bacteria were found in 74.2% of the patients and included 247 strains within 21 genera. ... | 2004 | 16701526 |
| european surveillance study on antimicrobial susceptibility of gram-positive anaerobic cocci. | gram-positive anaerobic cocci (gpac) are a heterogeneous group of microorganisms frequently isolated from local and systemic infections. in this study, the antimicrobial susceptibilities of clinical strains isolated in 10 european countries were investigated. after identification of 299 gpac to species level, the minimum inhibitory concentrations of penicillin, imipenem, clindamycin, metronidazole, vancomycin and linezolid were determined by the agar dilution method according to the clinical and ... | 2008 | 18180149 |
| use of bioinformatics and pcr in the search for abc transporter homology among various bacteria. | bioinformatics databases and search tools are utilised to produce polymerase chain reaction (pcr) primers for the amplification of an abc transporter gene from the clinically important anaerobe finegoldia magna. on sequencing, a 450 base pair amplicon showed homology with the amino acid transporter of enterococcus faecalis. little sequence data is available for f. magna and the newly isolated dna could be a useful tool in the identification of this organism in clinical specimens. | 2004 | 15649009 |
| isolation and characterisation of an equol-producing mixed microbial culture from a human faecal sample and its activity under gastrointestinal conditions. | only about one third of humans possess a microbiota capable of transforming the dietary isoflavone daidzein into equol. little is known about the dietary and physiological factors determining this ecological feature. in this study, the in vitro metabolism of daidzein by faecal samples from four human individuals was investigated. one culture produced the metabolites dihydrodaidzein and o-desmethylangolensin, another produced dihydrodaidzein and equol. from the latter, a stable and transferable m ... | 2004 | 15578160 |
| dna microarray analysis of predominant human intestinal bacteria in fecal samples. | a microarray method was developed for the detection of 40 bacterial species reported in the literature to be predominant in the human gastrointestinal tract. the 40 species include seven species each of bacteroides and clostridium, six species of ruminococcus, five species of bifidobacterium, four species of eubacterium, two species each of fusobacterium, lactobacillus and enterococcus, and single species each of collinsella, eggerthella, escherichia, faecalibacterium and finegoldia. three 40-me ... | 2004 | 15271382 |
| crystal structure and biological implications of a bacterial albumin binding module in complex with human serum albumin. | many bactericide species express surface proteins that interact with human serum albumin (hsa). protein pab from the anaerobic bacterium finegoldia magna (formerly peptostreptococcus magnus) represents one of these proteins. protein pab contains a domain of 53 amino acid residues known as the ga module. ga homologs are also found in protein g of group c and g streptococci. here we report the crystal structure of hsa in complex with the ga module of protein pab. the model of the complex was refin ... | 2004 | 15269208 |
| comparative analysis of the four rrna operons in finegoldia magna atcc29328. | there are four rrna operons rrna, rrnb, rrnc and rrnd on the genome of finegoldia magna (formerly peptostreptococcus magnus) atcc29328, which, in contrast to those of clostridia, are dispersed around the chromosome. using a bac library we determined the nucleotide sequences and structures of all four operons, including their flanking regions, and performed comparative analyses. we identified putative boxa sequences in the operons, which should be required for rrna transcription antitermination, ... | 2004 | 15053317 |
| phospholipid analogue profiles of peptostreptococcus, micromonas, and finegoldia species analysed by fast atom bombardment mass spectrometry. | species of peptostreptococcus cause a variety of infections, primarily abscesses of soft tissues, joints, and mucous membranes. the aim of this study was to compare the phospholipid analogue profiles of peptostreptococcus species, represented by p. anaerobius, p. asaccharolyticus, p. indolicus, p. lacrimalis, and p. prevotii; micromonas micros (p. micros) and finegoldia magna (p. magnus). after anaerobic growth on blood-faa, lipids extracted by chloroform-methanol (2:1 v/v) were purified, then a ... | 2001 | 15049457 |
| bacterial artificial chromosome library of finegoldia magna atcc 29328 for genetic mapping and comparative genomics. | we constructed a bacterial artificial chromosome (bac) library of finegoldia magna atcc 29328 dna to facilitate further genome analysis of f. magna. the bac library contained 385 clones with an average insert size of 55 kb, representing a 10.1-fold genomic coverage. repeated dna hybridization using primer sets designed on the basis of bac-end sequences yielded nine contigs covering 95% of the chromosome and two contigs covering 98% of the plasmid. the contigs were localized on the physical map o ... | 2003 | 14695451 |
| endocarditis caused by finegoldia magna (formerly peptostreptococcus magnus): diagnosis depends on the blood culture system used. | we present a patient with prosthetic valve endocarditis caused by finegoldia magna (formerly peptostreptococcus magnus). blood cultures in the bact/alert and bactec 9240 system were negative. we therefore tested different blood culture systems: f. magna grew in the septi-chek bhi-s and in the isolator, but not in the bact/alert system. | 2003 | 12967750 |
| detection and identification of bacterial dna in semen. | to detect and identify bacteria in semen by sequencing polymerase chain reaction (pcr)-amplified ribosomal rna gene regions (rdnas). | 2008 | 18191853 |
| in vitro and in vivo antibacterial activities of telithromycin. | telithromycin is one of the ketolides, characterised by a 3-keto group instead of l-cladinose and a c(11)-c(12) carbamate link by an alkyl chain to a pyridinum and imidazolium ring side chain. we evaluated in vitro and in vivo antibacterial activities of telithromycin against gynaecological pathogens. | 2003 | 12714813 |
| physical and genetic map of the finegoldia magna (formerly peptostreptococcus magnus) atcc 29328 genome. | a physical and genetic map of finegoldia magna (formerly peptostreptococcus magnus) atcc 29328 chromosome was constructed. the order of rare cleavage restriction fragments was determined by double digestion with the restriction enzymes i-ceui, sgrai, apai and pmei, cross-hybridization and apai-linking clones. the size of the circular chromosome of f. magna was estimated to be 1.9 mb. this strain also had a 200-kb megaplasmid. the chromosome contained four rrn operons, and the orientation of two ... | 2002 | 12023074 |
| complete genome sequence of finegoldia magna, an anaerobic opportunistic pathogen. | finegoldia magna (formerly peptostreptococcus magnus), a member of the gram-positive anaerobic cocci (gpac), is a commensal bacterium colonizing human skin and mucous membranes. moreover, it is also recognized as an opportunistic pathogen responsible for various infectious diseases. here, we report the complete genome sequence of f. magna atcc 29328. the genome consists of a 1,797,577 bp circular chromosome and an 189,163 bp plasmid (ppep1). the metabolic maps constructed based on the genome inf ... | 2008 | 18263572 |
| survey of bacterial diversity in chronic wounds using pyrosequencing, dgge, and full ribosome shotgun sequencing. | chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. these bacterial populations cooperate to promote their own survival and the chronic nature of the infection. few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. the use of 3 separate16s-based molecular amplifications followed by pyrosequencing, shotgun sanger sequencing, and denatur ... | 2008 | 18325110 |
| in vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections. | against 443 aerobic and anaerobic bacteria isolated from diabetic foot infections, ceftobiprole mics (microg/ml) at which 90% of the isolates tested were inhibited were as follows: methicillin-resistant staphylococcus aureus, 1; methicillin-susceptible s. aureus and staphylococcus lugdunensis, 0.5; anaerococcus prevotii, 0.125; finegoldia magna, 0.5; peptoniphilus asaccharolyticus, 1; peptostreptococcus anaerobius, 4; escherichia coli and enterobacter species, 0.125; klebsiella species, 2; and p ... | 2006 | 16982780 |
| [two cases of bartholin's gland abscesses caused by streptococcus pneumoniae and haemophilus influenzae]. | most of bartholin's gland abscesses have been thought to be caused by microorganisms found in opportunistic infections. however, we have encountered two very interesting cases of bartholin's gland abscesses caused by streptococcus pneumoniae and haemophilus influenzae, two major pathogens of respiratory tract infections. in the first case, since abscess formation was not observed due to disintegration, cefdinir (cfdn), 300 mg/day, t.i.d. for 5 days was administered. the treatment improved clinic ... | 2005 | 16276738 |
| alternate arrangement of ppl b3 domain and spa d domain creates synergistic double-site binding to vh3 and vkappa regions of fab. | in our previous study, a kind of novel hybrid immunoglobulin (ig)-binding proteins (ibps) was obtained with the characteristic structure of alternately arranged finegoldia magna (formerly peptostreptococcus magnus) protein l (p. magnus protein l, ppl) b3 domain (b3) and staphylococcal protein a (spa) d domain (d). in this study, two representative molecules of these novel proteins, ld3 (b3-d-b3) and ld5 (b3-d-b3-d-b3) (ld3/5), showed substantially higher affinity for igg-f(ab')2, igm, and iga th ... | 2008 | 18694300 |
| identification of a novel protein promoting the colonization and survival of finegoldia magna, a bacterial commensal and opportunistic pathogen. | anaerobic bacteria dominate the human normal microbiota, but strikingly little is known about these commensals. finegoldia magna is a gram-positive anaerobe found in the skin and at other non-sterile body surfaces, but it is also an opportunistic pathogen. this study describes a novel protein designated faf (f. magna adhesion factor) and expressed by more than 90% of f. magna isolates. the protein is present in substantial quantities at the f. magna surface but is also released from the surface. ... | 2008 | 18808384 |
| finegoldia magna, an early post-operative cause of infectious endocarditis: report of two cases and review of the literature. | we report two cases of infectious endocarditis (ie) on prosthetic valves caused by finegoldia magna. the diagnosis was obtained by detection of the bacterium in valvular biopsies using 16s rrna pcr amplication and sequencing, and prolonged culture. five other cases were previously published in the literature. following analysis of these seven cases, f. magna endocarditis presented as a subacute endocarditis, developing early (60 days) following valvular replacement (85%), with an elevated mortal ... | 2008 | 19084607 |
| sufa - a bacterial enzyme that cleaves fibrinogen and blocks fibrin network formation. | finegoldia magna is a member of the normal human bacterial flora on the skin and other non-sterile body surfaces, but this anaerobic coccus is also an important opportunistic pathogen. sufa was the first f. magna proteinase to be isolated and characterized. many bacterial pathogens interfere with different steps of blood coagulation, and here we describe how purified sufa efficiently and specifically cleaves fibrinogen in human plasma. sufa is both secreted by f. magna and associated with the ba ... | 2009 | 19118364 |
| rapid identification of gram-positive anaerobic coccal species originally classified in the genus peptostreptococcus by multiplex pcr assays using genus- and species-specific primers. | here, a rapid and reliable two-step multiplex pcr assay for identifying 14 gram-positive anaerobic cocci (gpac) species originally classified in the genus peptostreptococcus (anaerococcus hydrogenalis, anaerococcus lactolyticus, anaerococcus octavius, anaerococcus prevotii, anaerococcus tetradius, anaerococcus vaginalis, finegoldia magna, micromonas micros, peptostreptococcus anaerobius, peptoniphilus asaccharolyticus, peptoniphilus harei, peptoniphilus indolicus, peptoniphilus ivorii and pepton ... | 2003 | 12855723 |
| sufa of the opportunistic pathogen finegoldia magna modulates actions of the antibacterial chemokine mig/cxcl9, promoting bacterial survival during epithelial inflammation. | the anaerobic bacterium finegoldia magna is part of the human commensal microbiota, but is also an important opportunistic pathogen. this bacterium expresses a subtilisin-like serine proteinase, sufa, which partially degrade the antibacterial chemokine mig/cxcl9. here, we show that mig/cxcl9 is produced by human keratinocytes in response to inflammatory stimuli. in contrast to the virulent human pathogen streptococcus pyogenes, the presence of f. magna had no enhancing effect on the mig/cxcl9 ex ... | 2009 | 19628464 |
| murdochiella asaccharolytica gen. nov., sp. nov., a gram-stain-positive, anaerobic coccus isolated from human wound specimens. | two strains of previously unknown gram-stain-positive, anaerobic, coccus-shaped bacteria from human wound specimens were characterized using phenotypic and molecular taxonomic methods. comparative 16s rrna gene sequencing studies and distinguishable biochemical characteristics demonstrated that these two unknown strains, wal 1855c(t) and wal 2038e, are genotypically homogeneous and constitute a novel lineage within clostridium cluster xiii. there was 13-14 % 16s rrna gene sequence divergence bet ... | 2010 | 19666803 |
| a case of necrotizing fasciitis due to streptococcus agalactiae, arcanobacterium haemolyticum, and finegoldia magna in a dog-bitten patient with diabetes. | we report a case of necrotizing fasciitis involving streptococcus agalactiae, arcanobacterium haemolyticum, and finegoldia magna in a 36-yr-old female diabetic patient, which started after a minor dog bite to the toe of the patient. this case suggested that a trivial infection after a minor dog bite in an immunocompromised patient such as diabetes patient could result in a significant complication such as necrotizing fasciitis. the life-threatening infection was cured by timely above-the-knee am ... | 2008 | 18594170 |
| finegoldia magna: a forgotten pathogen in prosthetic joint infection rediscovered by molecular biology. | in this study, we describe 13 patients with prosthetic infections due to finegoldia magna (2% of our tested series). patients presented with either polymicrobial infection after an open fracture or nosocomial infection after recent prosthesis implantation. molecular techniques are critical for diagnosis, and recommended antibiotic prophylaxis has poor activity against f. magna. | 2009 | 19757992 |
| in vitro activity of tigecycline and comparators against a european compilation of anaerobes collected as part of the tigecycline evaluation and surveillance trial (test). | the tigecycline evaluation and surveillance trial (test) is a global surveillance study designed to monitor the in vitro activity of the broad-spectrum antimicrobial tigecycline against nosocomial- and community-acquired pathogens. in this study the in vitro activity of tigecycline against 1256 anaerobic pathogens collected across europe has been compared to the activity of several comparator antibiotics. the pathogens examined in this study included bacteroides, prevotella, anaerococcus, clostr ... | 2010 | 19883155 |
| recurrent abscesses due to finegoldia magna, dermabacter hominis and staphylococcus aureus in an immunocompetent patient. | a case of recurrent abscesses in an immunocompetent patient is reported, involving the opportunistic human pathogen dermabacter hominis, the virulent anaerobic pathogen finegoldia magna and staphylococcus aureus. | 2009 | 19332143 |
| [lumbar pyomyositis caused by finegoldia magna (peptostreptococcus magnus) in a patient with hepatitis c virus infection]. | | 2008 | 18373916 |
| the effects of circumcision on the penis microbiome. | circumcision is associated with significant reductions in hiv, hsv-2 and hpv infections among men and significant reductions in bacterial vaginosis among their female partners. | 2010 | 20066050 |
| activity of tigecycline and comparators against recent clinical isolates of finegoldia magna from europe. | a total of 206 clinical isolates of finegoldia magna were collected during the period 2007-2009 from six european countries. the majority of isolates were from body fluids (n = 83; 40.3%) or wounds (n = 82; 39.8%). all isolates were susceptible to tigecycline, meropenem, metronidazole and piperacillin / tazobactam, though susceptibility to penicillin (86.4-87.4%) and clindamycin (78.2-93.3%) were more variable. | 2010 | 20496091 |
| bacterial surface protein l binds and inactivates neutrophil proteins s100a8/a9. | finegoldia magna is an anaerobic bacterial species that is part of the normal human flora on all nonsterile body surfaces, but it is also a significant opportunistic pathogen causing a wide range of infections. some isolates of f. magna that are more frequently associated with clinical infection express protein l, a surface protein containing multiple homologous domains (b1-b5) that bind igs through interactions with ig l chains. the present study shows that the n-terminal a domain of protein l ... | 2009 | 19752232 |
| necrotising pneumonia due to finegoldia magna. | | 2010 | 21193258 |
| assessment of bacterial diversity in the cattle tick rhipicephalus (boophilus) microplus through tag-encoded pyrosequencing. | ticks are regarded as the most relevant vectors of disease-causing pathogens in domestic and wild animals. the cattle tick, rhipicephalus (boophilus) microplus, hinders livestock production in tropical and subtropical parts of the world where it is endemic. tick microbiomes remain largely unexplored. the objective of this study was to explore the r. microplus microbiome by applying the bacterial 16s tag-encoded flx-titanium amplicon pyrosequencing (btefap) technique to characterize its bacterial ... | 2011 | 21211038 |
| complete genome sequence of anaerococcus prevotii type strain (pc1). | anaerococcus prevotii (foubert and douglas 1948) ezaki et al. 2001 is the type species of the genus, and is of phylogenetic interest because of its arguable assignment to the provisionally arranged family 'peptostreptococcaceae'. a. prevotii is an obligate anaerobic coccus, usually arranged in clumps or tetrads. the strain, whose genome is described here, was originally isolated from human plasma; other strains of the species were also isolated from clinical specimen. here we describe the featur ... | 2009 | 21304652 |
| surface bacteriology of venous leg ulcers and healing outcome. | bacteria can be cultured from all venous leg ulcers (vlus) regardless of healing status, and the significance of a positive swab result in non-clinically infected ulcers is unknown. the aim of this study was to characterise the bacteriological flora of vlus by routine culture to determine whether the data generated had prognostic value. | 2010 | 20671048 |
| [Bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between April 2009 and March 2010]. | Bacteria isolated from surgical infections during the period from April 2009 to March 2010 were investigated in a multicenter study in Japan, and the following results were obtained. In this series, 671 strains including 16 strains of Candida spp. were isolated from 174 (79.1%) of 220 patients with surgical infections. Four hundred and eleven strains were isolated from primary infections, and 244 strains were isolated from surgical site infection. From primary infections, anaerobic Gram-negative ... | 2011 | 21861307 |
| identification of clinically important anaerobic bacteria by an oligonucleotide array. | anaerobic bacteria can cause a wide variety of infections, and some of these infections can be serious. conventional identification methods based on biochemical tests are often lengthy and can produce inconclusive results. an oligonucleotide array based on the 16s-23s rrna intergenic spacer (its) sequences was developed to identify 28 species of anaerobic bacteria and veillonella. the method consisted of pcr amplification of the its regions with universal primers, followed by hybridization of th ... | 2010 | 20129959 |
| a comparison of bacterial composition in diabetic ulcers and contralateral intact skin. | an extensive portion of the healthcare budget is allocated to chronic human infection. chronic wounds in particular are a major contributor to this financial burden. little is known about the types of bacteria which may contribute to the chronicity, biofilm and overall bioburden of the wound itself. in this study we compare the bacteriology of wounds and associated intact skin. wound and paired intact skin swabs (from a contralateral location) were collected. the bacterial diversity was determin ... | 2010 | 20461221 |
| the genome of th17 cell-inducing segmented filamentous bacteria reveals extensive auxotrophy and adaptations to the intestinal environment. | perturbations of the composition of the symbiotic intestinal microbiota can have profound consequences for host metabolism and immunity. in mice, segmented filamentous bacteria (sfb) direct the accumulation of potentially proinflammatory th17 cells in the intestinal lamina propria. we present the genome sequence of sfb isolated from monocolonized mice, which classifies sfb phylogenetically as a unique member of clostridiales with a highly reduced genome. annotation analysis demonstrates that sfb ... | 2011 | 21925113 |
| the b-cell superantigen finegoldia magna protein l causes pulmonary inflammation by a mechanism dependent on myd88 but not b cells or immunoglobulins. | objective and design: to determine whether finegoldia magna protein l (pl) causes lung inflammation and, if so, whether the response is dependent on its immunoglobulin (ig)-binding b-cell superantigenic property. material: pulmonary inflammatory reactions were analyzed at various time points after intratracheal administration of pl to various strains of mice. results: ... | 2012 | 22249932 |
| plasma half-life extension of small recombinant antibodies by fusion to immunoglobulin-binding domains (igbd). | many therapeutic proteins possessing a small size are rapidly cleared from circulation. half-life extension strategies have therefore become increasingly important to improve the pharmacokinetic and pharmacodynamic properties of protein therapeutics. here, we performed a comparative analysis of the half-life extension properties of various bacterial immunoglobulin-binding domains (igbd) derived from staphylococcus protein a (spa), streptococcus protein g (spg) and finegoldia (formerly peptostrep ... | 2011 | 22147690 |
| constitutive and inflammation-dependent antimicrobial peptides produced by epithelium are differentially processed and inactivated by the commensal finegoldia magna and the pathogen streptococcus pyogenes. | epithelial linings serve as physical barriers and produce antimicrobial peptides (amps) to maintain host integrity. examples are the bactericidal proteins midkine (mk) and brak/cxcl14 that are constitutively produced in the skin epidermal layer, where the anaerobic gram-positive coccoid commensal finegoldia magna resides. consequently, this bacterium is likely to encounter both mk and brak/cxcl14, making these molecules possible threats to its habitat. in this study, we show that mk expression i ... | 2011 | 21918193 |
| complete genome sequence of a serotype 11a, st62 streptococcus pneumoniae invasive isolate. | streptococcus pneumoniae is an important human pathogen representing a major cause of morbidity and mortality worldwide. we sequenced the genome of a serotype 11a, st62 s. pneumoniae invasive isolate (ap200), that was erythromycin-resistant due to the presence of the erm(tr) determinant, and carried out analysis of the genome organization and comparison with other pneumococcal genomes. | 2011 | 21284853 |
| anti-anaerobic activity of serum from patients treated with tigecycline for skin/soft tissue infections. | to gain additional data concerning the anti-anaerobic activity of tigecycline in serum, we analyzed blood samples from six patients with a complicated skin/soft tissue infection who were receiving iv tigecycline 50 mg every 12 h. venous blood samples were obtained after multiple doses of tigecycline at 1, 6 and 12 h after the initiation of a 1 h iv infusion. sera from these samples were tested to determine serum inhibitory and bactericidal activity over time against 4 anaerobic bacteria (bactero ... | 2011 | 21376823 |
| antimicrobial susceptibility of clinically relevant gram-positive anaerobic cocci collected over a three-year period in the netherlands. | the susceptibility of 14 species of 115 gram-positive anaerobic cocci (gpac) was determined for 14 antibiotics. to assure correct identification, strains were genotypically identified by fluorescence in situ hybridization and sequencing. susceptibility differences (mic₅₀ and mic₉₀) for penicillin g, clindamycin, tigecycline, levofloxacin, amoxicillin-clavulanic acid, cefoxitin, ertapenem, meropenem, metronidazole, and doxycycline were found for the three clinically most relevant gpac species: fi ... | 2010 | 21189338 |
| identification of gram-positive anaerobic cocci by maldi-tof mass spectrometry. | gram-positive anaerobic cocci (gpac) are part of the commensal microbiota of humans and are a phylogenetically heterogeneous group of organisms. to evaluate the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) for the identification of gpac, a database was constructed, using reference strains of commonly encountered gpac and clinical isolates of which the sequence of the 16s rrna gene was determined. subsequently, the database was validat ... | 2011 | 21292417 |
| postoperative mediastinitis due to finegoldia magna with negative blood cultures. | we report a case of finegoldia magna (formerly known as peptostreptococcus magnus) mediastinitis following coronary artery bypass in a 50-year-old patient. even if staphylococci remain the main causative organism of postoperative mediastinitis, the responsibility of anaerobic bacteria must be considered in cases of fever and sternal drainage with negative blood cultures. | 2009 | 19812272 |
| burden of emerging anaerobes in the maldi-tof and 16s rrna gene sequencing era. | the isolation of anaerobes from patients has declined in recent years, whereas their detection by molecular techniques has increased. in the present work, we analyzed the application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequencing to routine identification of anaerobes in clinical microbiology laboratory. we identified 544 isolates of 79 species by routine culture from deep samples in our hospital. maldi-tof ms allowed i ... | 2011 | 21672636 |