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use of enzyme-linked immunosorbent assay for the diagnosis of equine histoplasmosis farciminosi (epizootic lymphangitis).an enzyme-linked immunosorbent assay was evaluated for the detection of antibody in sera of equine naturally infected with histoplasma farciminosum 'epizootic lymphangitis'. ten sera from naturally infected horses were tested. a hydrogen peroxide abts mixture constituted the substrate. the reactions were read as the absorbance values measured at 405 nm using a spectrophotometer. the standard deviation and the average percentage of the absorbance values of the different serum samples were conside ...19854047125
[antigenic structure of histoplasma farciminosum. 3. double-diffusion in agar gel and immunoelectrophoresis]. 19734205663
histoplasma farciminosum, the aetiological agent of equine cryptococcal pneumonia. 19714939205
chemical and ultrastructural studies on the cell walls of the yeastlike and mycelial forms of histoplasma farciminosum.the cell wall of the yeast form of histoplasma farciminosum contains 13.2% beta-1,3-glucan, 1.0% galactomannan, and 25.8% chitin, whereas the cell wall of mycelial form has 21.8, 4.5, and 40%, respectively, for the same polymers. also, the cell wall of the yeast form contains alpha-1,3-glucan (13.5%) and an unidentified polymer (21.5%). chitin, one of the structural polymers of both yeast and mycelial cell walls, is identified as thin isolated fibers (4 nm wide) or in thick bundles (50 nm wide) ...19744850991
evaluation of berries of phytolacca dodecandra for growth inhibition of histoplasma capsulatum var. farciminosum and treatment of cases of epizootic lymphangitis in ethiopia.to evaluate the berries of phytolacca dodecandra (p. dodecandra) for its effect on histoplasma capsulatum var. farciminosum (hcf) and for the treatment of cases of epizootic lymphangitis (el).201223569960
ocular histoplasmosis due to histoplasma farciminosum in egyptian donkeys.in this study 19 donkeys suffering from face excoriations below the medial canthas and lesions in the lacrymal apparatus were subjected to clinical radiological and microbiological investigations. also histopathological examination of biopsy materials from ocular lesions were made. eleven out of 19 donkeys (57.9%) showed specific lesions in the lacrymal apparatus. mycological examination revealed the isolation of histoplasma cells from 10 out of 19 examined cases. the isolated histoplasma cultur ...19911795725
comparative evaluation of a chemiluminescent dna probe and an exoantigen test for rapid identification of histoplasma capsulatum.a chemiluminescent dna probe (accuprobe) assay developed by gen probe, inc., for the rapid identification of histoplasma capsulatum was evaluated and compared with the exoantigen test by using 162 coded cultures including histoplasma capsulatum var. capsulatum, histoplasma capsulatum var. duboisii, histoplasma capsulatum var. farciminosum, blastomyces dermatitidis, coccidioides immitis, paracoccidioides brasiliensis, and morphologically related saprobic fungi. each test uses a chemiluminescent, ...19921452692
studies on histoplasmosis farciminosi (epizootic lymphangitis) in egypt. isolation of histoplasma farciminosum from cases of histoplasmosis farciminosi in horses and its morphological characteristics.isolation of histoplasma farciminosum from five horses, showing typical signs of histoplasmosis farciminosi (epizootic lymphangitis) was successfully attempted. the mycelial form of h. farciminosum was isolated on sabouraud dextrose agar enriched with 2.5% glycerol, brain heart infusion (bhi) agar enriched with 10% horse blood and pplo dextrose glycerol agar. the last medium proved to be the most effective, both for primary isolation and subculturing of the fungus. it was found that on primary i ...19853842362
study on histoplasmosis (epizootic lymphangitis) in cart-horses in ethiopia.a cross-sectional study was conducted to determine the prevalence of histoplasma farciminosum in 2,907 carthorses using clinical and microbiological examinations at three towns (debre zeit, mojo and nazareth), central ethiopia, between december 1999 and january 2001. an overall prevalence of 26.2% (762/2,907) was recorded; the highest prevalence (39.1%) being recorded at mojo whereas the lowest (21.1%) was recorded at nazareth. the difference in prevalence among the three towns was highly signif ...200212441683
[antigenic structure of histoplasma farciminosum. ii. serological activity of various antigenic fractions of hist. farciminosum and hist. capsulatum in cf test. their antigenic relationship with other fungi]. 19734782071
phylogeny of dermatophytes and dimorphic fungi based on large subunit ribosomal rna sequence comparisons.the phylogeny of dermatophytes and dimorphic fungi was considered using the large-subunit of ribosomal rna (25s rrna). aligned sequences of 595 nucleotides covering the two most divergent domains d1 and d2, permitted a comparison of phylogenetic relationships at different levels. the dimorphic species (onygenaceae) were significantly separated from dermatophytes (arthrodermataceae) and from a third group including geophilic or very weakly pathogenic species (onygenaceae and gymnoascaceae). on a ...19947844699
[antigenic structure of histoplasma farciminosum. i. preparation of antisera and antigens and assessment of their immunologic activities]. 19734798693
epizootic lymphangitis in horses: a review of the literature.epizootic lymphangitis is a relatively common infectious disease of horses and other liquids in certain parts of the world. the infection rate varies according to the geographic area and the age of the animal. the disease is most commonly characterised by a cord-like appearance of the subcutaneous lymphatic and cutaneous pyogranulomas, the discharge from which contains spherical or pear-shaped bodies of the causal agent, histoplasma farciminosum. diagnosis can be made by the demonstration of typ ...199910588013
study on the survival of histoplasma farciminosum in the environment. 19836646170
in vitro antifungal activity of hamycin against histoplasma farciminosum. 19695397911
histofarcin test for the diagnosis of epizootic lymphangitis in ethiopia: development, optimisation, and validation in the field.histofarcin, a skin test antigen for the diagnosis of epizootic lymphangitis, was locally produced from the mycelial form of histoplasma capsulatum var. farciminosum (hcf) in disease-endemic districts of ethiopia and tested for its application in the field between april 2002 and may 2003. the test was evaluated using 108 mules, 84 in endemic and 24 in disease-free districts. microscopic and mycological examinations of clinical lesions were used as the "gold standard" for the validation of the te ...200616490721
aspects of the dimorphism of histoplasma farciminosum: a light and electron microscopic study.within 48h following the induction of mycelial to yeast-like phase conversion of histoplasma farcininosum, randomly occurring hyphal cells were observed to contain multiple nuclei and markedly increased numbers of mitochondria. yeast-like cells arose as buds from swollen tips of terminal hyphae, as sessile buds along the hyphae, and as buds from chlamydospores. yeast-like cells were characterized by the presence of numerous buds over the surface of the mother cell. bud scars were evident in the ...19751162540
preliminary trial on the reproducibility of epizootic lymphangitis through experimental infection of two horses.epizootic lymphangitis (el) was experimentally reproduced in four horses that had been purchased from an el-free district. two horses were injected with either 0.2 ml of the yeast form of histoplasma capsulatum var. farciminosum (hcf) in pus (horse 1), or 0.2 ml (ca. 20 mg) of a suspension in saline of the mycelial form (horse 2), both into the pre-scapular and pre-femoral lymph nodes, with scarification of the skin of the left hind limb, conjunctiva of the right eye and the nasal membrane of th ...200616125424
study of equine histoplasmosis farciminosi and characterization of histoplasma farciminosum.a detailed clinical and mycological study of horse infections with histoplasma farciminosum was conducted for the first time in the middle east. the disease seems to prevail in endemic form in the region. in all of the cases studied the infection involved only the cutaneous lymphatics and skin tissue with extension to the regional draining lymph glands. the disease seems to impose serious economic impact in the infected areas. full description and thorough characterization of both the mycelial f ...19836879394
the effect of amphotericin-b, 5-fluorocytosine and nystatin on histoplasma farciminosum in vitro. 19846720163
development and evaluation of a molecular diagnostic method for rapid detection of histoplasma capsulatum var. farciminosum, the causative agent of epizootic lymphangitis, in equine clinical samples.histoplasma capsulatum var. farciminosum, the causative agent of epizootic lymphangitis (ezl), is endemic in parts of africa. diagnosis based on clinical signs and microscopy lacks specificity and is a barrier to further understanding this neglected disease. here, a nested pcr method targeting the internal transcribed spacer (its) region of the rrna operon was validated for application to equine clinical samples. twenty-nine horses with signs of ezl from different climatic regions of ethiopia we ...201627707938
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