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[experimental study of the stability of avirulent properties and the immunogenicity of suppressor revertants of streptomycin-dependent salmonella mutants].the authors aimed at the experimental confirmation of avirulent properties of suppressor revertants of streptomycin-dependent salmonella mutants and also at the study of their immunogenicity. stability of the loss of virulence by the suppressor revertants was established by studying the virulence of the subcultures isolated from them, differing by the genotype. repeated passage of suppressor revertants of s. typhimurium rev8, s. enteritidis rev5, s. pullorum rev through the biological objects hi ...1976797191
resistance of mice to krebs ascites tumour, sarcoma s180 and pc6 plasmacytoma after immunisation with salmonella enteritidis 11rx.resistance to krebs ascites tumour, sarcoma s180 (ascitic form) and pc6 plasmacytoma can be induced with prior immunisation with live salmonella enteritidis 11rx. one thousand to ten thousand more tumour cells are needed to give an ld50 after immunisation. resistance to pc6 could be recalled 137 days after intravenous immunisation with 11rx protein antigen intraperitoneally as measured by prolonged survival or monitoring the death of 5[131i]-iodo-2'-deoxyuridine labelled tumour cells in vivo. th ...1976797376
local and systemic effects in the non-specific tumour resistance induced by attenuated salmonella enteritidis 11rx in mice.suppression of growth of a number of different murine tumours, injected subcutaneously or into the foot pad, was induced by prior intraperitoneal or intravenous immunisation with salmonella enteritidis 11rx. the effect of immunisation on tumour growth was relatively minor, but could be enhanced by addition of 11rx antigen preparations to the suspensions of tumour cells used for challenge. the significance of these findings is discussed.1976797377
a role for antibody in the expression of cellular immunity to salmonella typhimurium c5.immunisation of mice with alcohol-killed salmonella typhimurium c5 vaccine before infection with salmonella enteritidis 11rx enhanced the ability of these mice to control and eliminate a challenge dose of salmonella typhimurium c5, compared to mice which only received live salmonella enteritidis 11rx organisms. when alcohol-killed salmonella enteritidis se795 vaccine was used instead of the salmonella typhimurium c5 vaccine, this effect was not observed. these observations imply that both humora ...1976797378
the role of thymus-derived cells in immunity to salmonella infection.the ability of thymus-derived (t) cell depleted mice to eliminate a dose of the normally avirulent 11rx strain of salmonella enteritidis was compared to that of normal mice by following the fate of the challenge organisms in the liver and spleen of both types of mice. although the mice did not require normal numbers of t cells to survive infection with salmonella enteritidis 11rx, t cell depletion reduced the ability of mice effectively to eliminate the organism from their tissues. in addition, ...1976797379
phage typing system for salmonella enteritidis.a system is described for the phage typing of salmonella enteritidis. the system was developed using a number of bacteriophages that were isolated from sewage.1976788636
effect of capsular polysaccharide of klebsiella pneumoniae on host resistance to bacterial infections. ii. effects on peritoneal leukocytes of normal mice and mice infected with virulent salmonella enteritidis.in normal mice, the total count of peritoneal leukocytes was markedly decreased after intraperitoneal (i.p.) injection of the capsular polysaccharide of klebsiella pneumoniae (cps-k) depending on the dosage injected. this decrease was mainly due to the depletion of macrophages, and a decrease in the number of lymphocytes occurred to a lesser extent. cps-k in relatively smaller doses mobilized polymorphonuclear neutrophilic leukocytes (pmn) into the peritoneal fluid but it decreased them transien ...1976792533
[immunological investigation on refratory nature of salmonella infections]. 1975806640
nature of "memory" in t-cell-mediated antibacterial immunity: anamnestic production of mediator t cells.mice that survived an immunizing infection with listeria monocytogenes remained specifically resistant to lethal secondary infection for several months. this acquired, long-lived state of resistance was not dependent on activated macrophages that remained after the primary response. it depended, instead, on an acquired long-lived capacity on the part of immunized mice for generating mediator t cells faster and in larger numbers than normal mice. the number of mediator t cells generated in respon ...1975811558
[enteritis-salmonellae as a cause of unusual infection courses]. 1976819821
survival of bacteria in seawater using a diffusion chamber apparatus in situ.a microbiological survival chamber for in situ environmental studies involving microorganisms of public health significance was developed. the autoclavable chamber was provided with a supportive base for proper flow orientation and a battery-powered stirring mechanism for continuous internal agitation. the performance of the chamber and its ancillary units was evaluated in estuarine waters by diffusion studies and survival tests with eight species of bacteria isolated from environmental sources. ...1976820259
susceptibility of clinically important gram-negative bacilli to 15 antibiotics. comparison of patterns in poland and federal republic of germany.three hundred and sixty strains of gram-negative bacilli (escherichia coli, salmonella enteritidis, enterobacter cloacae, providencia, indole-positive and indole-negative proteus, klebsiella pneumoniae, pseudomonas aeruginosa and serratia marcescens), were isolated by random in poland and germany, and tested by agar dilution method for susceptibility to 15 antimicrobial agents, including six cephalosporin derivatives, colistin, gentamycin and tobramycin, three newer tetracyclines, ampicillin and ...1976824896
[immunological efficacy of live streptomycin-dependent salmonellae vaccines used orally in experiment on monkeys].experiments were conducted on 99 monkeys; a study was made of the reactogenic properties and the immunological efficacy of the streptomycin-dependent s. enteritidis and s. typhimurium mutants in oral application. the results of clinical, bacteriological, pathomorphological and fluorescent-serological studies demonstrated that the vaccines in the doses under study were areactogenic for monkeys in 5-fold oral administration and promoted development of marked immunity. the greatest immunizing effec ...1976827161
toxic effects of cadmium microparticles on the respiratory system. an experimental study on rats and mice.an experimental study on rats and mice. scand. j. work environ. & health 3 (1977) 116--121. experiments on male, specific pathogen-free rats (193) and mice (66) are reported. the test and control animals received a single 15-min exposure to cadmium (cadmium oxide) and aluminum (alumina) microparticles, respectively. the air cadmium content was 10 mg/m3, and 4 microgram of this metal was retained by the lungs of the rats. the animals were observed for 24 days. the following significant difference ...1977910121
ribonucleic acid synthesis in normal and immune macrophages after antigenic stimulus.macrophage ribonucleic acid (rna) synthesis is an important metabolic process intimately related to the function of these cells. mouse peritoneal macrophage rna was extracted with phenol in the presence of bentonite and electrophoresed on composite agarose-polyacrylamide gels. the pulse-chase technique was used to follow the precursor relationships in macrophage ribosomal rna (rrna) maturation. the rrna species at 18s and 28s appeared at 15 and 45 min, respectively, after rna synthesis was halte ...1976971940
[study of an explosive outbrake of gastroenteritis by salmonella enteritidis, infantis serotype]. 19761029851
[experience in controlling the salmonella carrier state on a pedigree poultry breeding farm].systematic microbiologic control was carried out in the 1972-1975 period on an elite poultry farm whereas from the 23,724 samples studied, taken from objects of the epizootic chain forage-birds-hatchery, 78 cultures of salmonella organisms of 14 species or 0.32 per cent of the total number of samples were isolated. a trend was observed toward the year-to-year drop in the number of positive findings of salmonellae, and by the end of 1975 the results were negative. this fact was backed by the inve ...19761030870
pathogenesis of salmonella-associated arithritis in the rat.the distribution of joint lesions in rats with salmonella-associated arthritis (ssa), as determined in a detailed survey, resembles to a great extent the pattern of small joint involvement in human rheumatoid arthritis. such lesions, though regularly induced in the rat by the intravenous injection of live s.enteritidis, could not be evoked by the heat-killed organisms injected by various routes with and without extrinsic adjuvants. efforts to transfer saa from sensitized donors to either normal ...19751089597
blast transformation of rabbit b-derived lymphocytes by a mitogen extracted from nocardia.nocardia water soluble mitogen (nwsm) is known to stimulate mouse and rabbit lymphoid cells and to act selectively on murine b-derived lymphocytes. in this paper, evidence is presented that nwsm is also a mitogen for rabbit bursal equivalent cells and does not bring about blast transformation of thymus-derived rabbit cells. pretreatment of rabbit spleen lymphocytes with antibody directed against rabbit thymus lymphocyte antigen (anti rtla serum) and with complement did not affect the strong incr ...19751090651
effects of bacterial lipopolysaccharide on the induction and expression of cell-mediated immunity. i. depression of the afferent arc.bacterial lipopolysaccharide (lps), being a b cell mitogen, enhances antibody production. when given prior to an injection of sheep red blood cells (srbc) it caused a premature but not significantly increased production of antibody forming cells in regionally stimulated lymph nodes. there was a corresponding depression of t cell activity, as measured by the relative levels of delayed-type hypersensitivity (dth) in normal and lps-treated mice. although lps also induced hyperplasia of the reticulo ...19751090661
[salmonella infections in infants and small children hospitalized in the years 1968-1972]. 19751090982
the role of granulocytes in the activation of intravascular coagulation and the precipitation of soluble fibrin by endotoxin.this study examines the role of neutrophils (pmn) in the pathogenesis of endotoxin-induced microclot formation. it is intended to clarify whether granulocytes are involved in endotoxin-induced activation of intravascular coagulation (generation of soluble fibrin) and/or in endotoxin-induced precipitation of soluble fibrin. precipitation of soluble fibrin was achieved by injection of endotoxin into ancrod-infused rabbits with circulating soluble fibrin (first model). activation of intravascular c ...19751091310
plasma substitute induced impairment of the reticuloendothelial system function.currently used plasma substitutes are large colloids and are partly engulfed by reticuloendothelial (re) cells. this study was designed to investigate the magnitude and duration of the possible res dysfunction induced by plasma substitutes. the following substances were studied in both normal and burned (ld-10) cba mice: allogen albumin 3%, and commercial preparations of dextran 40, dextran 70, hydroxyethyl starch, gelatin and pvp. infusion volume was 20 ml/kg and i.v. infusion time 1 hour. anim ...19751092112
ineffectiveness of lipopolysaccharide for preventing the tolerance induction in bone marrow-derived lymphoid cells with dinitrophenyl-poly-l-(glutamic acid, lysine).the effect of endotoxin or lipopolysaccharide (lps) on tolerance induction in bone marrow-derived lymphoid cells (b cells) was investigated. dinitrophenylated amino acid copolymer-l-(glutamic acid, lysine) (dnp-gl) acts as a potent tolerogen on normal and dnp-primed b cells. lps significantly enhanced the anti-sheep red blood cell plaque-forming cell (anti-srbc pfc) response that occurred after the immunization with a low dose of srbc. lps did not induce the primary anti-dnp pfc response after t ...1976792534
leukocyte inhibiting factor (lif) production from human lymphocytes stimulated by bacterial lipopolysaccharides.leukocyte inhibiting factor (lif) release from lymphocytes cultures stimulated by bacterial lipopolysaccharides (lps) has been investigated. human lymphocytes cultured in the presence of e, coli or s. enteritidis lps were able to release in the supernatants a substance with the following properties: a) same elution pattern of bovine serum albumin (mw 69000) when filtered on sephadex g-100; b) it migrates on disc electrophoresis a an albumin; c) not dialyzable; d) not cytotoxic; e) inhibition of ...1976798596
[infection with "enteritis salmonella" at non-intestinal sites (author's transl)].so-called "enteritis salmonella" was isolated in five cases from extra-intestinal specimens (about 3% of all primary salmonella isolations in one year). the organisms were: s. typhimurium, s. enteritidis, s. java, s. panama, and s. infantis. in four of the five cases the clinical picture was not that of a salmonella infection. focal manifestations were a knee-joint empyema after long-term treatment with glucocorticoids, pleuritis in hodgkin's disease, osteomyelitis of the head of the tibia, and ...19751097228
endotoxin-sensitive membrane component of human platelets. 19751097934
[relation of the immune response in rats to the state of the lysosomes and intensity of protein synthesis].in stimulation of an immune response to salmonellae in rats by dibasol and methyluracil and its depression by chlorophos and an amine salt of 2,4-dichlorphenoacetic acid it was found that the intensity of the immune response depended more on the level of protein biosynthesis than on the state of lysosomes at the period preceding the immunization.19751098336
septic arthritis in sickle-cell thalassemia. pathophysiology of impaired response to infection.the knee of a boy with sickle-cell thalassemia became infected with salmonella enteritidis, sensitive to ampicillin. doses of ampicillin that achieved anti-bacterial titers of 32 in synovial fluid failed to eradicate the organism. the synovial fluid exhibited severe hypoxia, hypercapnia, and lactic acidosis. methods were developed to estimate the oxygen saturation of blood in regional venous capillaries, the degree of periarticular sickling, changes in the viscosity of local blood, and the artic ...19751098670
[resistance to antibiotics such as furazolidone and sulfathiazole of s. typhimurium and s. enteritidis strains isolated from humans and animals in 1960-1973]. 19751099366
specific antibodies and heterologous antigensin monofactor sera.a concentrated monofactor serum 9 (wellcome lot k 8028) precipitated against proteins from 2 different strains of salmonella enteritidis and from a strain of s. dublin, as well as against the proteinic moiety of the somatic antigen from s. typhi 0 901; no polysaccharide from the strains, sharing factor 9, precipitated against this serum. a concentrated monofactor serum 20 (wellcome lot k 6733) precipitated against the proteins from s. emek (8, 20), but not against its polysaccharide. a monofacto ...19751099401
postnatal development of pyrogenic sensitivity in guinea pigs.despite evidence of thermoregulatory ability from birth, neonates generally are unable to develop fever when challenged with endotoxin. this could be due to their small capacity for heat storage. to test this possibility, the pyrogenicity of s. enteritidis endotoxin (2 mug/kg, iv) was measured at both room (ta = 25 degrees c) and neutral (tn = 29-33 degrees c, depending on age) temperatures in 0- to 32-day-old unanesthetized guinea pigs, reared from birth at about 24 degrees c. control guinea pi ...19751100593
unusual enterobacteriaceae: a salmonella cubana that is urease positive.this is the first report of a naturally occurring salmonella that is urea positive. the strain was identified as salmonella cubana and it was typical in all biochemical, serological, and bacteriophage reactions, except that is produced urease strongly.19751100646
complement-mediated bactericidal system: evidence for a new pathway of complement action.the early components of human complement (c1, c4, and c2) plus certain serum euglobulins will kill pathogenic strains of shigella sonnei. serum from patients with hereditary c3 deficiencies and specific antiserums to c3, c5, and c6 were utilized to demonstrate the absence of requirements for late-acting complement components in this unusual bactericidal system.19751101380
[phage typing of strains of s. enteritidis isolated in bulgaria].studied were a total of 221 strains of salmonella enteritidis isolated from birds, pigs, cattle, dogs, rodents, forage, environment, food products of animal origin, and human beings, in terms of their phage typing, in order to study the epizootiology of the foci of infection. twelve phages were established: 2a, 3a, 4a, 5a, 6a, 7a, 8a, 9a, 15, 19, 20 and 21. most widely distributed proved phage type 6a, followed by phage type 2a. the remaining phages occured more rarely, and some of them--15, 19, ...19751101519
profuse fimbriae conferring o-inagglutinability to several strains of s. typhi-murium and s. enteritidis isolated from pasta products. cultural, morphological, and serological experiments.during the last 2 years several o-inagglutinable salmonella strains were isolated from pasta products the typing of which presented great difficulties. serological and morphological tests (by electron microscope) revealed a fimbrial antigen which was thermolabile to a certain degree. this antigen represents (in corroboration of j.p. dugiud) a special type of fimbriae of salmonella and is morphologically different from type 1 and typ3-2 fimbriae previously described. it is probably an abnormal mu ...19751101574
[sensitivity to bacteriophage es18 of strains of "s. dublin", "s. enteritidis" and "s. blegdam" and related serotypes (author's transl)].sensitivity to bacteriophage es18 may be used as an additional test for identification of strains of some serotypes of salmonella. in the d group, the great majority (100/103) of the strains of s. enteritidis (h = g,m:-) and 7/7 strains of s. blegdam (h = g,m,q:-) are resistant. on the contrary, all the studied strains (111) of s. dublin (h = g,p:-) either vi+ or vi- are sensitive, as those of s. gallinarum-pullorum (61). two strains of s. kiel (possibly derivated from s. dublin) are sensitive a ...19751103676
reiter's disease in three boys.three cases of reiter's disease occurring in boys under the age of 16 are reported. one of these presented with a salmonella enteritidis diarrhoea. this conforms to the 'dysenteric' form of reiter's disease usually seen in europe and rarely reported in england. another presented with a monarticular arthritis of the knee, and the third has developed a chronic relapsing erosive arthritis as a result of sexually acquired reiter's disease--an occurrence not previously reported in this age group. we ...19751103755
scanning electron microscopic studies on the microstructure of s and r type colonies of salmonella and shigella groups. 1976802350
[case of plating out the causative agent of intestinal infections from the oral cavity]. 19751106082
[some problems of nomenclature and taxonomy of the enterobacteriaceae]. 19751106093
blastogenesis of human lymphocytes by endotoxin.lipopolysaccharides of s. typhimurium, s. enteritidis and e. coli at wide range of concentrations were used to induce blastogenesis in human and mouse (nude) lymphocytes. human lymphocytes from peripheral blood showed positive response to at least one source of lps (stimulation index of 2-9). the optimum concentration resulting in maximum stimulation varied with different individual, sources and concentrations of lps used. lymphocytes from cord blood failed to respond to lps, but had positive re ...19751107213
[role of the mammary gland in the development of immunity with the intracisternal administration of antigen]. 19751108387
[study of salmonella cultures. iv. immunogenicity of salmonella resistant to antibiotics].three avirulent and 5 slightly virulent for birds mutants of salmonella enteritidis and salmonella braenderup, resistant to streptomycin (s), chlorteracyclin (ct), and chloramphenicol (ch) were used in the production of live vaccines. the latter were tested for immunogenicity on albino mice and birds. on the eighth and 15th day following vaccination the protein fractions in the blood serum of the birds were followed up through electrophoresis on agar gel, while the agglutinin titer was determine ...19751108410
canine salmonellosis: a review and report of dog to child transmission of salmonella enteritidis.dogs have been shown to harbor 53 salmonellae serotypes. multiple simultaneous infections with 2 to 4 serotypes have been observed. the prevalence of canine salmonellosis may be a high as 27 per cent. salmonella typhimurium and s. anatum are the most common etiologic agents. dogs commonly experience a sub-clinical course of salmonellosis. some investigators state that the dog may serve as a source of human infections. a few reports in the literature have documented this fact. the transmissions ...19761108681
motility-indole-lysine medium for presumptive identification of enteric pathogens of enterobacteriaceae.detection of lysine decarboxylase activity is a useful supplement to reactions on triple sugar-iron (tsi) and urea agars in the initial examination of suspected pathogenic isolates from fecal cultures. owing to the added value of motility and indole production in the differentiation of enteric pathogens, we prepared and evaluated a motility-indole-lysine (mil) medium. the following 890 organisms were tested: 264 shigella, 2 edwardsiella, 182 salmonella enteritidis, 235 s. typhi, 3 arizona, 32 ye ...19751176632
[possibilities of differentiation of the specific positive reactions for pullorum disease].studied were sera obtained from three groups of birds experimentally infected with salmonella gallinarum-pullorum. salmonella enteritidis, and salmonella heidelberg, resp., using the whole serum agglutination reaction, the slow test-tube reaction (at 37 degrees and 50 degrees c), and the agar gel precipitation reaction as modified by aoki as well as the spot-test of williams. it was found that with the use of a typhus antigen it is not possible to differentiate the positive coagglutination react ...19751210001
the immunological basis of acquired cellular resistance in some infectious diseases caused by intracellular parasites. ii. antigen modulation produced on cells of animals infected with several species of intracellular parasites other than salmonella enteritidis. 19751234923
monoclonal antibodies against salmonella porins: generation and characterization.monoclonal antibodies (mabs) were generated against porins, one of the major outer membrane proteins of salmonella typhi. six clones, designated mp1, mp2, mp3 (igg2ak), mpn4, mpn6 (igg1k) and mpn5 (igg2bk) were characterized by enzyme immunoassay (elisa) for their reactivity to porins from s. typhi, salmonella paratyphi a, s. paratyphi b, s. paratyphi c, salmonella choleraesuis, salmonella enteritidis, salmonella krefeld, salmonella panama, salmonella typhimurium, escherichia coli b, shigella fl ...19921280248
nucleotide sequence of the nfaa gene encoding the antigen 8786 adhesive factor of enterotoxigenic escherichia coli.the nucleotide sequence of a 714-bp dna fragment containing the enterotoxic escherichia coli (etec) adhesive factor 8786 structural gene, designated nfaa, revealed an open reading frame of 498 bp encoding a polypeptide of 166 amino acids. primer-extension experiments showed that the nfaa gene is within a single transcription unit. no homology was found with the etec adhesive factors already sequenced. in contrast, a homology with salmonella enteritidis fimbrin sef 14 was observed.19921281130
food poisoning at a wedding reception. 19921284928
food poisoning at a religious event. 19921284929
food poisoning in two homes for the elderly. 19921284930
raw eggs and body builders. 19921285093
salmonella enteritidis pt 8 and turkey meat. 19921285145
effect of sample selection from experimentally contaminated hatching eggs and freshly hatched chicks on salmonella enteritidis detection rate.the conventional culture methods of hatching eggs using shell and/or egg contents for detection of salmonella organisms give mostly unsatisfactory results. the aim of the present study is to evaluate selection of other samples and techniques of culturing hatching eggs and freshly hatched chicks. this current study provides the best evidence of salmonella enteritidis in artificially contaminated eggs (layer type) by using enrichment broth in empty egg shell samples in comparison to culturing samp ...19921289043
salmonella enteritidis infection in the sudan.twenty-one salmonella enteritidis isolates were recovered from several poultry farms in three states in the sudan over an eighteen-month period. the infection was disseminated from a distributing company which had imported infected fertilized eggs and parent stock. the sudan s. enteritidis epidemic which devastated many poultry farms during 1990 was attributable and concurrent to that in europe in particular and throughout the world in general.19921301625
molecular phylogenetic typing of pandemic isolates of salmonella enteritidis.salmonella enteritidis is now the most common salmonella serovar in many countries. we have used cloned dna probes to analyze genome interrelationships between strains chosen to represent the current s. enteritidis pandemic, and included designated type strains of the seven subspecies of salmonella in order to compare the levels of discrimination of probes. dna sequence divergence and rearrangements were analyzed in and around the rfa, fim and umudc loci, and around insertion sites of the salmon ...19921311276
genetic relationships among strains of salmonella enteritidis in a national epidemic in switzerland.a collection of salmonella enteritidis strains isolated in switzerland (1965-90) was characterized. the phage type and plasmid profile of isolates were compared with the copy number and insertion loci of the dna insertion element is200. three clonal lines of s. enteritidis were identified by is200 profile; the various phage types were subtypes reproducibly associated with one of these lines. all human and poultry isolates contained a 38 mda plasmid which hybridized with a mouse virulence-associa ...19921316285
cloning, sequencing and expression of the nhaa and nhar genes from salmonella enteritidis.na+/h+ antiporter activity is wide-spread and plays essential physiological roles. we found that several enterobacteriaceae share conserved sequences with nhaa, the gene coding for an e. coli antiporter. a delta nhaa strain, which is sensitive to na+ and li+, was used to clone by complementation a dna fragment from salmonella enteritidis which confers resistance to the ions. the cloned fragment increased na+/h+ antiport activity in membranes isolated from strains carrying the respective hybrid p ...19921317153
identification of novel loci affecting entry of salmonella enteritidis into eukaryotic cells.there are an estimated 2 million cases of salmonellosis in the united states every year. unlike the incidence of many infectious diseases, the incidence of salmonellosis in the united states and other developed countries has been rising steadily over the past 30 years, and the disease now accounts for 10 to 15% of all cases of acute gastroenteritis in the united states. the infecting organism is ingested and must traverse the intestinal epithelium to reach its preferred site for multiplication, ...19921317843
molecular detection of infectious bursal disease virus by polymerase chain reaction.the polymerase chain reaction (pcr) technique was applied to the detection of infectious bursal disease virus (ibdv). reverse transcription followed by the pcr was used to amplify a portion of ibdv genome. a set of primers that specify a 150-base-pair segment of ibdv genome was chosen from an australian strain of ibdv. standard challenge strain and variant strains a, d, e, g, and gls-5 of ibdv serotype 1 and oh strain of serotype 2 from infected bursae were subjected to reverse transcription, fo ...19921320861
an unusual pagc::tnphoa mutation leads to an invasion- and virulence-defective phenotype in salmonellae.two phenotypes believed to contribute to the pathogenesis of salmonella infections are macrophage survival and invasion of epithelial cells. it was recently observed that the salmonella macrophage survival factor pagc has significant amino acid similarity to the yersinia invasion factor ail. this observation raised the possibilities that macrophage survival is in part determined by the pathway of entry and that pagc confers an entry mechanism that does not trigger the microbicidal activities of ...19921323535
macrophage antimicrobial functions in a chicken mhc chromosome dosage model.macrophages respond to certain inflammatory signals with a marked increase in respiratory burst and the production of reactive oxygen intermediates; these metabolites play an essential role in the destruction of invading microorganisms. in this study, macrophage antibacterial inflammatory responses were compared among chickens having two (disomic), three (trisomic), or four (tetrasomic) copies of the major histocompatibility complex (mhc)-encoding microchromosome (b15 haplotype). phorbol myrista ...19921326019
meningitis caused by salmonella enteritidis in an adult. 19921332352
effect of endotoxic shock on basal and insulin-mediated na+/k(+)-pump activity in rat soleus muscle.this study evaluated basal and insulin-mediated na+/k+ transport in skeletal muscle during endotoxic shock. fasted male holtzman rats (80-100 g) were killed 5 hr after the i.v. injection of saline (control) or 20 mg/kg salmonella enteritidis endotoxin. 86rb+ uptake into the cellular compartment of soleus muscle was determined in the presence and absence of 100 mu/ml insulin. the rate coefficient and rate of 22na+ efflux and muscle intracellular 22na+ content (in percentage of total muscle 22na+) ...19921338036
clinical relevance of herpes simplex virus in the throat of elderly with salmonella enteritidis gastroenteritis.it is not known whether the presence of herpes simplex virus in the throat of elderly patients with severe gastroenteritis and pulmonary implications is of clinical relevance. we cultured throat swabs and faeces of elderly patients with (n = 11) and without (n = 12) severe salmonella gastroenteritis for viruses and bacteria to study the aetiology of respiratory complications. complement fixation titers for anti-herpes simplex antibodies in paired sera were also ascertained. throat swabs of 6 out ...19921341452
oxygen-independent antimicrobial activity against salmonella enteritidis of specially activated macrophage with living vaccine.characteristics of peritoneal macrophages recovered from mice infected with two attenuated strains ser and jena of salmonella enteritidis were compared. strong resistance against lethal infection with a virulent strain 116-54 of s. enteritidis was seen in a group of mice immunized with strain ser, but not in a group of mice immunized with strain jena as well as in a control group. peritoneal macrophages from mice immunized with strain ser showed an enhanced salmonella-killing activity, an increa ...19921344009
[bacteria associated with diarrheic episodes in young camels in niger].during diarrhoeic episodes of young camels in niger, bacteria such as salmonella enteritidis, e. coli, staphylococcus sp., clostridium perfringens and other bacteria were isolated from stool samples. bacteria roll in this pathology is discussed, and a treatment is proposed, compared with antibiogram results.19921345079
the crl protein activates cryptic genes for curli formation and fibronectin binding in escherichia coli hb101.curli are thin, coiled, temperature-regulated fibres on fibronectin-binding escherichia coli. the subunit protein of curli was highly homologous at its amino terminus to sef-17, the subunit protein of thin, aggregative fimbriae of salmonella enteritidis 27655 strain 3b, suggesting that these fibres form a novel class of surface organelles on enterobacteria. e. coli hb101 is non-curliated and unable to bind soluble, iodinated fibronectin. the phenotypically cryptic curlin subunit gene, csga, in h ...19921357528
role of type 1 and type 3 fimbriae on the adherence and pathogenesis of salmonella enteritidis in mice.through hemagglutination tests two isogenic strains of salmonella enteritidis were shown to possess type 1 fimbriae (strain v) and type 1 and type 3 fimbriae (strain a). the two strains bound to human buccal and mouse small intestine epithelial cells. strain a attached to the epithelial cells more readily and in larger numbers in comparison to strain v. adherence of both strains were sensitive to the presence of d-mannose and pretreatment of the epithelial cells with tannic acid did not promote ...19921357531
involvement of the upper respiratory tract in orally induced salmonellosis in mice.the possible involvement of the tissues of the head and neck during the early pathogenesis of orally or intragastrically induced murine salmonellosis was examined. viable counts of salmonella in the local lymphoid tissues and draining nodes indicated that the ileal peyer's patches were primarily involved in the development of a subsequent enteric fever, irrespective of the method of oral challenge. contamination of drinking water with a high concentration of salmonellae produced infection of the ...19751092770
an erythrocyte-sensitising antigen from vibrio cholerae.erythrocyte-sensitising antigens (ae) were prepared from vibrio cholerae serotypes, from el-tor vibrio, escherichia coli and salmonella enteritidis by digesting the organisms with naoh followed by precipitation with alcohol. when ae was used in indirect haemagglutination (iha) tests, the results in a number of cases were somewhat more sensitive and more specific than those obtained in classical agglutination tests. no cross reactions occurred between v. cholerae serotypes and e. coli and s. ente ...19751092860
antimicrobial resistance and r-factor transfer among isolates of salmonella in the northeastern united states: a comparison of human and animal isolates.the antimicrobial susceptibility of 718 isolates of salmonella from humans and of 688 isolates from animals was examined. of the 46 different serotypes among the isolates from humans, salmonella typhimurium accounted for 34%. thirty percent of isolates were resistant to one or more antibiotic(s). resistance to streptomycin was most common; resistance to tetracycline was next most common. over 50% of isolates of s. typhimurium and salmonella newport were resistant to four antibiotics. resistance ...19751104721
characterisation of monoclonal antibodies against a fimbrial structure of salmonella enteritidis and certain other serogroup d salmonellae and their application as serotyping reagents.a panel of 13 monoclonal antibodies from different hybridomas was produced against a novel salmonella fimbrial antigen expressed predominantly by salmonella enteritidis strains. the specificity of the monoclonal antibodies to this antigen (sef14) was confirmed by enzyme-linked immunosorbent assay (elisa) using purified sef14, immune electron microscopy and, with 11 monoclonal antibodies, the identification of a repeating protein subunit (14,300kda) on the antigen. blocking-elisa with the monoclo ...19921361237
plasmid analysis and epidemiology of salmonella enteritidis infection in three commercial layer flocks.ninety-six s. enteritidis isolates obtained from three commercial layer flocks in 1988-90 were examined following dna extraction, restriction enzyme digestion, and gel electrophoresis for plasmid size profiles and restriction fragment length polymorphisms (rflps). the s. enteritidis isolates from the three flocks had three, eight, and two different plasmid profiles, respectively. only four isolates from one flock lacked plasmids. a 36-megadalton (mda) (54-kilobase) plasmid was present in 73% of ...19921362492
[reactive arthritis caused by salmonella]. 19921366167
hospital outbreak of salmonella enteriditis infection--ontario. 19921366254
hydroxypropyl cellulose/poly(ethylene glycol)-co-poly(propylene glycol) aqueous two-phase systems: system characterization and partition of cells and proteins.novel aqueous polymeric two-phase systems are described. these systems are formed by mixing hydroxypropyl cellulose (molecular mass 100,000, trade name klucel l) with poly(ethylene glycol)-co-poly(propylene glycol) copolymer [molecular mass 6,500, poly(propylene glycol) content 50% w/w, trade name pluronic p105], in a saline buffer. the phase diagram was measured and the interfacial tensions, phase separation times, and lower phase viscosities of three phase systems having constant pluronic p105 ...19921368966
construction of salmonella strains with both antigen o4 (of group b) and antigen o9 (of group d).a salmonella live vaccine causing both o4- and o9-specific immune responses would be of use, but no reported salmonella serotype has both of these o antigens. constructed salmonella typhimurium strains with an rfb (o-antigen-specifying) gene cluster of type d in the chromosome and one of type b in an f'-rfb+ factor, and those with the reverse combination reacted strongly with both anti-o4 (and anti-o5) and anti-o9 sera and, if they carried reca, could be maintained in this state by growth condit ...19921372316
structural and immunochemical studies of the lipopolysaccharides of salmonella strains with both antigen o4 and antigen o9.two salmonella hybrid strains, sl5313 (salmonella typhimurium with a d.rfb+ gene cluster) and sl5396 (s. enteritidis with a b.rfb+ gene cluster), each expressing both o-antigen 4 (of serogroup b) and o-antigen 9 (of serogroup d) were studied by immunofluorescence using a mixture of o4-specific mouse monoclonal and o9-specific rabbit polyclonal antibodies. bound antibodies, detected by anti-mouse antibody labelled with fluorescein isothiocyanate and anti-rabbit antibody labelled with tetramethylr ...19921372317
cloning and nucleotide sequence of the gene (citc) encoding a citrate carrier from several salmonella serovars.the nucleotide sequence of the citc coding for the citrate carrier in several salmonella serovars has been determined, and the amino acid sequence of the carrier protein was deduced. the predicted citrate carrier from salmonella pullorum and salmonella enteritidis consists of 446 amino acids with a molecular weight of 47,621, whereas that from salmonella dublin is the same 446 amino acids with the slightly different molecular weight of 47,591, because 1 amino acid residue was substituted. the pr ...19921374406
role of nitric oxide in the oxidant stress during ischemia/reperfusion injury of the liver.the potential role of nitric oxide (no) and its reaction product with superoxide, peroxynitrite, was investigated in a model of hepatic ischemia-reperfusion injury in male fischer rats in vivo. pretreatment with the no synthase inhibitor nitro-l-arginine (10 mg/kg) did neither affect the post-ischemic oxidant stress and liver injury during the initial reperfusion phase nor the subsequent infiltration of neutrophils into the liver and the later, neutrophil-induced injury phase. furthermore, no ev ...19921375973
salmonella enteritidis-specific monoclonal antibodies.three monoclonal antibodies (mabs) were derived that are specific for salmonella enteritidis. such antibodies are of interest because reagents that specifically identify s. enteritidis are potentially useful for the diagnosis and detection of this pathogen. immunization of balb/c mice with intact, unfixed, ultraviolet-killed s. enteritidis permitted the derivation of a collection of hybridomas among which were found three mabs: 1053, 1110, and 1170. each mab reacted with six independent field is ...19921378262
serum, disc and egg elisa for the serodiagnosis of salmonella gallinarum and s. enteritidis infections in chickens.an elisa was evaluated for the serodiagnosis of fowl typhoid and paratyphoid due to salmonella enteritidis in chickens. the hot phenol: water lipopolysaccharide (lps) extract of salmonella was used as the antigen. chicken serum, eggs and discs impregnated with chicken blood were tested for the presence of antibodies against salmonella factor 'o' 9 antigen. the substrate and chromogen used were hydrogen peroxide and orthophenylenediamine respectively. serological results from the experimentally a ...19921381107
[focal infections caused by non-typhi salmonella: a review of our case series and comparison with other series].nine cases of local infection due to non typhi salmonella enterica, some of them of unusual localization, in 8 patients (mean age 64.9 +/- 12.4 years) attended in zamora's virgen de la concha hospital over a period of five years, are described. focal salmonellosis represented 1.5% of non-typhi salmonellosis cases in that period (9 out of 606 detected cases). 6 of the 8 patients (75%) showed a predisposing disease. in two patients the previous existence of gastroenteritis due to salmonella was as ...19921386936
[molecular epidemiology of salmonella enterica poisoning: correlation of the serotype and protein profile and plasmid dna analysis].food-borne diseases nosocomial outbreaks due to salmonella enterica strains have a high incidence nowadays. we describe here an outbreak that occurred in july 1990 in the malaga university hospital, that only involves one shift of health care workers. salmonella enterica was detected in stool samples from hcw and a first analysis revealed the presence of two different antibiotic susceptibility patterns (resistotypes) among isolated strains. two months after the outbreak started, the cnvis (majad ...19921391014
effect of faecal extract on the growth of salmonella enteritidis in artificially contaminated hens' eggs.1. salmonella enteritidis pt 4 grew in eggs stored at 25 degrees c, but not at 10 degrees c. 2. the incidence of generalised infection of the egg contents (greater than 10(6) salmonellas/ml) was greater in eggs inoculated with cells suspended in faecal extract compared to those with cells in ringer's solution. 3. the removal of most of the iron did not decrease the growth-promoting effect of the faecal extract.19921393670
techniques for the isolation of salmonellas from eggs.1. when the contents of 4 or more naturally-contaminated intact eggs were combined, the isolation rate of salmonella enteritidis was improved by extending incubation at 37 degrees c from 24 to 48 h before sub-culture. 2. the isolation rate of salmonellas from raw homogenised whole egg was significantly increased by the inclusion of novobiocin and cefsulodin in the primary culture media. 3. rappaport vassiliadis broth was found to be superior to selenite as a selective enrichment medium.19921393671
use of competitive exclusion to protect newly-hatched chicks against intestinal colonisation and invasion by salmonella enteritidis pt4.1. the recommended dose of a commercial competitive exclusion (ce) product (broilact) was given orally to newly-hatched broiler chicks to protect them against oral challenge by salmonella enteritidis pt4. 2. in 5 replicate trials, half of the birds thus treated and half from untreated control groups were examined for salmonellas at 5 d and the other half at 12 d after challenge. 3. caecal contents were examined quantitatively while heart, liver and spleen samples were examined qualitatively by e ...19921393673
serological responses of chickens experimentally infected with salmonella enteritidis pt4 by different routes.commercially reared chickens were challenged with salmonella enteritidis phage-type (pt) 4 by aerosol, or via the conjunctiva. inhalation of 2.9 x 10(2) or 4.2 x 10(3) s. enteritidis resulted in the production of igg antibodies to the lipopolysaccharide (lps) of s. enteritidis pt4. when the aerosol inoculum was increased to 2.4 x 10(5) bacteria per bird the antibody produced were predominantly of the igm-class. chickens challenged with 10(3) s. enteritidis pt4 via the conjunctiva mounted only a ...19921397118
endotoxin tolerance is associated with altered gtp-binding protein function.previous studies have suggested that guanine nucleotide regulatory (g) proteins modulate endotoxin-stimulated peritoneal macrophage arachidonic acid (aa) metabolism. endotoxin-stimulated metabolism of aa by peritoneal macrophages is decreased in endotoxin tolerance (rogers et al. prostaglandins 31: 639-650, 1986). these observations led to a study of g protein function and aa metabolism by peritoneal macrophages in endotoxin tolerance. endotoxin tolerance was induced by the administration of sub ...19921400011
comparison of four different enzyme-linked immunosorbent assays for serological diagnosis of salmonella enteritidis infections in experimentally infected chickens.the program for the eradication of salmonella enteritidis from chickens in the netherlands is based on bacteriological examination of breeding flocks. there is a great need for a specific and sensitive serological screening test. for that purpose, we developed four different enzyme-linked immunosorbent assays (elisas), i.e., an indirect elisa with s. enteritidis flagellin, an indirect elisa with s. enteritidis lipopolysaccharide, a double-antibody sandwich blocking elisa that uses monoclonal ant ...19921400954
[studies on genetic differentiation of salmonella enteritidis isolates by plasmid profile].since 1989, the number of salmonellosis cases caused by s. enteritidis has increased considerably in japan. genetic differentiation of 385 strains isolated from january 1982 to december 1988 and january 1989 to april 1991 were used for plasmid profiles. plasmids were found in 377 out of 385 strains; therefore, only 8 strains carried no plasmid. among 377 strains, 15 different plasmid profile types (op-1 to op-15) were classified. the most common plasmid profile types from 1982 to 1988 were op-7 ...19921402111
salmonella-associated with deep vein thrombosis: report of a case and review of the literature. 19921402120
platelet-derived growth factor reduces the inhibitory effects of lipopolysaccharide on gingival fibroblast proliferation.lipopolysaccharide from a variety of bacterial sources is known to inhibit gingival fibroblast proliferation and synthetic activity and has been implicated in the pathogenesis of periodontal inflammation. however, it may be involved not only in pathogenesis but also be responsible for delayed wound healing following periodontal therapy. the aim of this investigation was to determine whether the inhibitory effect of lps on gingival fibroblast proliferation could be reversed by growth factors. hum ...19921403578
microbiological and histopathological effects of an induced-molt fasting procedure on a salmonella enteritidis infection in chickens.a study was undertaken to determine if a 2-week feed-removal protocol, as is used by industry to induce a molt in aging hens, would affect the course of a salmonella enteritidis infection. white leghorn hens aged 69-84 weeks were deprived of feed to induce a molt, and on day 4 of the fast, the birds were orally infected with 5 x 10(6) s. enteritidis. s. enteritidis organisms were enumerated in the spleen on day 6 and from the alimentary tract on days 7, 14, 21, 28, and 35. little difference was ...19921417591
the role of mice in the epizootiology of salmonella enteritidis infection on chicken layer farms.a microbiological survey of 10 mice-infested poultry farms was conducted to determine the role of mice in the epizootiology of s. enteritidis infection. five of the farms were rated as clean of s. enteritidis and five as contaminated based on culture results of environmental samples for s. enteritidis. of 2103 environmental samples and 715 mice and rats tested, 5.1% and 16.2%, respectively, were culture-positive for s. enteritidis. on contaminated farms, s. enteritidis was isolated from 24.0% of ...19921417592
occurrence of salmonella enteritidis in the u.s. commercial egg industry: report on a national spent hen survey.in order to estimate the prevalence and distribution of salmonella enteritidis in u.s. commercial egg-production flocks, a survey of spent laying hens was conducted over a 3-month period. seven of the 10 largest spent-hen processing plants in the united states participated. ceca were sampled twice weekly from birds presented for slaughter at these plants. samples were cultured for salmonella and s. enteritidis, and s. enteritidis isolates were phage-typed. overall, 23,431 pooled cecal samples we ...19921417594
salmonella enteritidis infection in two species of psittaciformes.in 1990, salmonella enteritidis phage type 4 was recovered from two young (less than 20-week-old) lilac-crowned amazon parrots (amazona finschi schlater), one in tennessee and one in kansas. the parrot from tennessee was treated for a plugged naris and anorexia before the s. enteritidis infection was discovered. the parrot from kansas exhibited signs of septicemia and died within 24 hours of examination. an apparently healthy green-cheeked conure (pyrrhura molinae) on the same premises as the pa ...19921417612
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