Publications

development and validation of species-specific molecular diagnostic tool for opisthorchis felineus (digenea, opisthorchiidae) metacercariae.	opisthorchis felineus (family opisthorchiidae) is a parasitic flatworm representing a serious threat to humans in some countries. opisthorchiasis occurs after consumption of raw or undercooked cyprinid fish infected by the metacercarial stage of the parasite. due to its small size, detection of the parasite in fish fillet is time-consuming and difficult. furthermore, isolated metacercariae can be identified to genus but not to species level using morphological features and molecular techniques a ...	2017	27914324
opisthorchiasis in western siberia: epidemiology and distribution in human, fish, snail, and animal populations.	opisthorchiasis is a widespread helminth infection in russia. the largest opisthorchiasis endemic focus in the world is the ob river watershed in western siberia. the main causative agent of this condition is the liver fluke, opisthorchis felineus. in addition, another liver fluke species in the opisthorchiidae family, metorchis bilis, causes a symptomatically similar disease, metorchiasis. despite a long research history going back to 1927, opisthorchiasis remains a serious problem in russia, a ...	2016	27939972
opisthorchis felineus negatively associates with skin test reactivity in russia-europrevall-international cooperation study.	most studies on the relationship between helminth infections and atopic disorders have been conducted in (sub)tropical developing countries where exposure to multiple parasites and lifestyle can confound the relationship. we aimed to study the relationship between infection with the fish-borne helminth opishorchis felineus and specific ige, skin prick testing, and atopic symptoms in western siberia, with lifestyle and hygiene standards of a developed country.	2016	28032359
protozoan and helminth parasite fauna of free-living croatian wild wolves (canis lupus) analyzed by scat collection.	the european wolf (canis lupus) is a large carnivore species present in limited areas of europe with several small populations still being considered as endangered. wolves can be infected by a wide range of protozoan and metazoan parasites with some of them affecting free-living wolf health condition. on this account, an epidemiological survey was conducted to analyze the actual parasite fauna in croatian wild wolves. in total, 400 individual faecal samples were collected during field studies on ...	2017	28043382
secretion of thioredoxin peroxidase protein of cat liver fluke opisthorchis felineus during modeling of experimental opisthorchiasis.	mechanisms of thioredoxin peroxidase secretion by opisthorchis felineus were studied in vivo and in vitro. specific antibodies were obtained and used for western blotting and immunohistochemical detection in syrian hamster model of opisthorchiasis. secreted thioredoxin peroxidase protein was accumulated in the worm incubation medium under conditions of oxidative stress and in bile duct cells of hamsters with chronic opisthorchiasis.	2017	28429227
first report and molecular identification of opisthorchis viverrini infection in human communities from lower myanmar.	opisthorchis viverrini is endemic in the south east asian region, especially in cambodia, lao people's democratic republic, vietnam and thailand, but there have been no previous records from myanmar. during stool surveys of rural populations in three regions of lower myanmar, opisthorchis-like eggs were found in 34 out of 364 (9.3%) participants by stool microscopy after using the modified formalin-ether concentration technique. dna was extracted from these positive stool samples and a portion o ...	2017	28472153
anthelmintic activity of cytochrome p450 inhibitors miconazole and clotrimazole: in-vitro effect on the liver fluke opisthorchis felineus.	discovery of drugs for the treatment of opisthorchiasis and schistosomiasis is a high priority. the basic metabolic cytochrome p450 (cyp) system in parasitic flatworms contains a single gene. cyp of the liver fluke opisthorchis felineus, the causative agent of opisthorchiasis, is important for survival of the worm, so it may be a promising target for therapeutics against liver fluke infection. the aims of this study were: (i) to analyse in-vitro anthelmintic activity of various cyp inhibitors us ...	2017	28527633
magnetic resonance imaging and spectroscopy for differential assessment of liver abnormalities induced by opisthorchis felineus in an animal model.	european liver fluke opisthorchis felineus, causing opisthorchiasis disease, is widespread in russia, ukraine, kazakhstan and sporadically detected in the eu countries. o. felineus infection leads to hepatobiliary pathological changes, cholangitis, fibrosis and, in severe cases, malignant transformation of bile ducts. due to absence of specific symptoms, the infection is frequently neglected for a long period. the association of opisthorchiasis with almost incurable bile duct cancer and rising i ...	2017	28708894
ultrastructural characters of the spermatozoon of the liver fluke opisthorchis viverrini (poirier, 1886) (opisthorchiidae).	the present study records the ultrastructural organization of the mature spermatozoon of opisthorchis viverrini by means of transmission electron microscopy. the spermatozoon of o. viverrini is a filiform cell, tapered at both extremities. it exhibits the characteristics of type iv spermatozoon of digeneans, namely with two axonemes of the 9+'1' trepaxonematan pattern, external ornamentation of the plasma membrane associated with cortical microtubules that are in the posterior part of the anteri ...	2017	28725936
abc transporters in the liver fluke opisthorchis felineus.	atp-binding cassette transporters (abc transporters) are essential components of normal cellular physiological machinery in all eukaryotic and prokaryotic species, including parasites. some abc transporters, e.g., p-glycoproteins, are involved in the efflux of toxins and xenobiotics from the cell. at present, nothing is known about abc transporter genes in epidemiologically important liver flukes from the opisthorchiidae family, including european liver fluke opisthorchis felineus. opisthorchias ...	2017	28729070