isolation of a cdna for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor srk of brassica campestris.srk is a plant receptor kinase involved in the self-incompatibility system of brassica species. during a cdna screening for the phosphoproteins from a stigma expression library, a clone encoding the nucleoside diphosphate kinase iii (bc-ndpk iii) was obtained. after in vitro phosphorylation assays with recombinant proteins, bc-ndpk iii contained mostly phosphoserine. by contrast, the kinase domain of srk contained phosphoserine and phosphothreonine, both of which were significantly increased by ...200211886897
assessment of imidacloprid in brassica environment.imidacloprid was applied as seed treatment (gaucho 70 ws, 5 and 10 g ai kg(-1) seed) and foliar spray (confidor 200 sl, 20 and 40 g ai ha(-1)) at 50% pod formation stage on mustard (brassica campestris linn.) to control mustard aphid, lipaphis erysimi kalt. it was detectable upto 82 and 96 days in plants after sowing from lower and higher doses of seed treatment. however, it dissipated faster and became nondetectable after 7 and 15 days of foliar treatments from lower and higher rates of applica ...200111599725
purification and characterization of dihydroxyacetone phosphate reductase from immature seeds of brassica campestris l.dihydroxyacetone phosphate reductase (dhap reductase) was purified to apparent electrophoretic homogeneity with about 24% recovery from immature seeds of brassica campestris using (nh(4))(2)so(4) fractionation, affinity chromatography, gel filtration and adsorption chromatography. the purified enzyme with molecular mass of about 62 kda was a dimer with subunit molecular mass of 32 kda. the enzyme exhibited maximum activity at ph 7.5 and was highly specific for nadh and dhap. typical michaelis-me ...200111448735
[production and cytogenetics of intergeneric hybrids between ogura cms brassica campestris var. purpuraria and raphanus sativus].crosses between ogura cms brassica campestris var. purpuraria (aa, 2n = 20) and raphanus sativus (rr, 2n = 18) were made and many intergeneric hybrids were produced. the f1 seedlings did not show chlorosis at low temperature. when red raphanus sativus varieties were used as male parent, the leaf petiole and leaf vein of f1 plants were purple, and when white raphanus sativus varieties were used as male parent, the leaf petiole and leaf vein of f1 plants were not purple. all the f1 plants had whit ...200111431989
controlling self-incompatibility by co2 gas treatment in brassica campestris: structural alteration of papillae cell and differential gene expression by increased co2 gas.self-imcompatibility is a controlling genetic mechanism to prevent self-pollination for chinese cabbage (brassica campestris), one of the major vegetable crops in korea. to maintain inbred lines of the crop plant, a method in that high co2 gas is treated to the pistils to overcome the self-incompatibility and thereby self-pollens can successfully make germination and fertilization has been widely used in seed companies. despite the common utilization of this method, any molecular and cellular st ...200111355699
isolation and characterization of a class i slg gene from chinese cabbage (brassica campestris).in brassica species, self-incompatibility in the recognition reaction between self and non-self pollens is determined by two genes, slg and srk, at the s locus. we have cloned and characterized a genomic dna fragment containing a complete open reading frame of the slg gene from chinese cabbage. the genomic clone, named bcslg2, was found to possess the region that shares a homology of 77% in amino acid identity with the slg46 gene of brassica campestris. northern blot analysis revealed that the b ...200011211873
purification and characterization of cytosolic pyruvate kinase from developing seeds of brassica campestris l.cytosolic pyruvate kinase (atp: pyruvate phosphotransferase, ec; pkc) was purified to apparent homogeneity with about 22% recovery from developing seeds of brassica campestris using (nh4)2so4 fractionation, deae-cellulose chromatography, gel filtration through sepharose-cl-6b and affinity chromatography through reactive blue sepharose-cl-6b. the purified enzyme with molecular mass of about 214 kda was a heterotetramer with subunit molecular mass of 55 and 57 kda. the enzyme showed maxim ...200010983413
fungicides cytotoxicity expressed in male gametophyte development in brassica campestris after in vitro application of converted field doses.a simple method to determine the toxicity of fungicides on male gametophyte in brassica campestris subsp. oleiferae is described. the calculation of fungicide concentration used in the test is derived from doses used in field application. the expression of regression curves and calculation of regression equations require the logarithmic transformation of fungicide concentration. the range of the sensitivity of the method is very wide. the minimal concentration detected as significantly different ...200010927128
persistence and safety evaluation of alphamethrin on mustard (brassica campestris linn.). 200010885997
phytogrowth-inhibitory activities of beta-dolabrin and gamma-thujaplicin, hinokitiol-related compounds and constituents of thujopsis dolabrata sieb. et zucc. var hondai makino.beta-dolabrin and gamma-thujaplicin isolated from thujopsis dolabrata sieb. et zucc. var hondai makino, like hinokitiol, showed strong phytogrowth-inhibitory activities, and their growth-inhibitory activities were as high as that of sodium 2,4-dichlorophenoxyacetate used as a positive control. in particular, the phytogrowth-inhibitory activity of gamma-thujaplicin was strong and it completely inhibited the germination of this seed of brassica campestris l. subsp. rapa hook f. et anders at the co ...200010823681
highly divergent sequences of the pollen self-incompatibility (s) gene in class-i s haplotypes of brassica campestris (syn. rapa) l.self-incompatibility (si) enables flowering plants to discriminate between self- and non-self-pollen. in brassica, si is controlled by the highly polymorphic s locus. the recently identified male determinant, termed sp11 or scr, is thought to be the ligand of s receptor kinase, the female determinant. to examine functional and evolutionary properties of sp11, we cloned 14 alleles from class-i s haplotypes of brassica campestris and carried out sequence analyses. the sequences of mature sp11 prot ...200010812061
the pollen determinant of self-incompatibility in brassica campestris.many flowering plants possess self-incompatibility (si) systems that prevent inbreeding. in brassica, si is controlled by a single polymorphic locus, the s locus. two highly polymorphic s locus genes, slg (s locus glycoprotein) and srk (s receptor kinase), have been identified, both of which are expressed predominantly in the stigmatic papillar cell. we have shown recently that srk is the determinant of the s haplotype specificity of the stigma. srk is thought to serve as a receptor for a pollen ...200010677556
cloning and sequencing of an apparently recombinant promoter for napin gene from brassica campestris genomic library and its evolutionary significance.from a genomic library of brassica campestris (brown sarson cv. b54), we have cloned and sequenced about 2 kb of upstream regulatory region from one of the 2s albumin-coding gene family. the sequence has several seed-specific promoter motifs. a sequence alignment of the 5' flanking regions of the available brassica 2s storage protein genes showed that our sequence is a double crossover recombinant product of the two members of the napin gene family. a possible explanation of this fact is that br ...199910650722
inhibitory activities of 2-pyridinecarboxylic acid analogs on phytogrowth and enzymes.five 2-pyridinecarboxylic acid-related compounds (1, 2 and 5-7) showed germination inhibition against the seed of brassica campestris l. subsp. rapa hook fil et anders at a concentration of 5.0 x 10(-4) m. these compounds also demonstrated inhibitory activity on the growth of the root of this plant at a concentration of 3.0 x 10(-4) m; among these compounds, 2-pyridylacetic acid (5) showed the strongest inhibitory activity, and the effect was slightly stronger than that of sodium 2,4-dichlorophe ...199910598034
traditional processing treatments as a promising approach to enhance the functional properties of rapeseed (brassica campestris var. toria) and sesame seed (sesamum indicum) meals.different processing treatments were applied to rapeseed and sesame seed meals, and the functional properties of these products were assessed. all treatments except puffing for both meals and pressure cooking in sesame meal increased water absorption capacity (wac). fat absorption capacity (fac) of rapeseed meals was enhanced significantly by all treatments. the full-fat meals of both sources showed maximum protein solubility when fermented and minimum protein solubility when pressure-cooked. ge ...199910552614
genomic organization of the s locus: identification and characterization of genes in slg/srk region of s(9) haplotype of brassica campestris (syn. rapa).in brassica, two self-incompatibility genes, encoding slg (s locus glycoprotein) and srk (s-receptor kinase), are located at the s locus and expressed in the stigma. recent molecular analysis has revealed that the s locus is highly polymorphic and contains several genes, i.e., slg, srk, the as-yet-unidentified pollen s gene(s), and other linked genes. in the present study, we searched for expressed sequences in a 76-kb slg/srk region of the s(9) haplotype of brassica campestris (syn. rapa) and i ...199910471721
purification and characterizaton of plastidic pyruvate kinase from developing seeds of brassica campestris l.plastidic pyruvate kinase (atp: pyruvate phosphotransferase, ec was purified to near homogeneity as judged by native page with about 4% recovery from developing seeds of brassica campestris using (nh4)2so4 fractionation, deae-cellulose chromatography, gel filtration through sepharose-cl-6b and affinity chromatography through reactive blue sepharose-cl-6b. the purified enzyme having molecular mass of about 266 kda was quite stable and showed a broad ph optimum between ph 6.8-7.8. typica ...199810412228
constituents of the tapetosomes and elaioplasts in brassica campestris tapetum and their degradation and retention during brassica anthers during microsporogenesis, the tapetum cells contain two abundant lipid-rich organelles, the tapetosomes possessing oleosins and triacylglycerols (tags), and the elaioplasts having unique polypeptides and neutral esters. b. campestris, for its simplicity of possessing only the aa genome and one predominant oleosin of 45 kda, was studied. in the developing anthers, the lipids and proteins of the tapetosomes and elaioplasts were concomitantly accumulated but selectively degraded ...199810036772
analysis of the four cox2 genes found in turnip (brassica campestris, brassicaceae) turnip mitochondria the cox2 gene is partially contained within a 2-kb (kilobase) direct repeat that is the site of homologous recombination events that generate subgenomic molecules. because of its partial presence within the direct repeat, the turnip cox2 gene exists in four genomic contexts, two on the master chromosome and one on each of the subgenomic circles. in order to gain information on the expression of plant mitochondrial genes that exist in multiple genomic contexts and on plant ...199821684900
enzymes of glycolytic and pentose phosphate pathways in cytosolic and leucoplastic fractions of developing seeds of brassica campestris.distribution of the enzymes of glycolytic and pentose phosphate pathways were studied in cytosolic and leucoplastic fractions of the developing seeds of brassica. leucoplasts were isolated using a discontinuous percoll gradient. intactness of leucoplasts was checked by adp-glucose pyrophosphorylase assay in presence and absence of triton x-100. no contamination by microbodies, mitochondria and cytosol was observed as assessed by measuring the activities of marker enzymes. the recovery, latency a ...19979425748
[supercritical-co2 fluid extraction of natural ve and fatty oil from the oil foot of brassica campestris and gc-ms analysis].natural ve and fatty oil from the oil foot of brassica campestris are extracted by supercritical-co2. after saponification and esterification these acidesters and natural ve in the extraction are analyzed by gc-ms. the results show that the fatty acids from sfe-co2 contain 10 components which of oleic acid, linodeic acid and linolenic acid are main components, linodenic acid and linoleic acid of them are increased signifantly, at the same time, natural ve can be extracted from the materials and ...199712572454
1,8-cineole inhibits root growth and dna synthesis in the root apical meristem ofbrassica campestris l.1,8-cineole is a volatile growth inhibitor produced bysalvia species. we examined the effect of this allelopathic compound on the growth of other plants usingbrassica campestris as the test plant. cineole inhibited germination and growth ofb. campestris in a dosedependent manner. whenb. campestris was grown for 5 days with various concentrations of cineole, the length of the roots was found to be shorter as the concentration of cineole increased, whereas the length of the hypocotyl remained cons ...199727520037
new acylated anthocyanins from brassica campestris var. chinensis.two new acylated anthocyanins were isolated from beninabana, brassica campestris var. chinensis, in addition to two known anthocyanins. the structures were established by spectral analyses.199727396743
a novel proline-rich glycoprotein associated with the extracellular matrix of vascular bundles of brassica petioles.a panel of monoclonal antibodies (mac204, mac236, mac265) which recognise extracellular matrix glycoproteins implicated in plant-microbe interactions has been used to study glycoprotein antigens in petioles of turnip (brassica campestris l.). while mac204 recognised two glycoproteins (gp120 and gp45) with apparent m(r) 120,000 and 45,000 in petiole extracts made with 2-amino-2-(hydroxymethyl)-1,3-propanediol (tris) buffer containing sodium dodecyl sulfate, mac236 recognised gp120 but not gp45, a ...19979177049
evaluation of 515 expressed sequence tags obtained from guard cells of brassica an attempt to examine the transcripts expressed in a single cell type and to unveil the physiology of guard cells at the molecular level, we generated 515 expressed sequence tags (ests) from a directional cdna library constructed from guard-cell protoplasts of brassica campestris l. ssp. pekinensis. a comparative analysis of the guard-cell ests against the national center for biotechnological information non-redundant protein database revealed that 133 ests (26%) have significant similarity t ...19979177047
genes encoding subunit 6 of nadh dehydrogenase and subunit 6 of atp synthase are co-transcribed in maize mitochondria.the mitochondrial nad6 gene of maize was identified and mapped 1 kb downstream from the atp6 gene. it encodes a 220 amino-acid polypeptide. using northern hybridization experiments and rt-pcr analysis, we showed that both nad6 and atp6 are co-transcribed in maize mitochondria. rna editing of the mitochondrial nad6 transcript was studied by cdna sequencing. twelve edited sites were identified at the same positions as those already identified in the wheat mitochondria nad6 transcript. alignments o ...19979000382
fatty acid synthesis by isolated leucoplasts from developing brassica seeds: role of glycolytic intermediates as the source of carbon and energy.fatty acid synthesis from na [1-14c] acetate in leucoplasts isolated from developing seeds of brassica campestris was completely dependent on exogenous supply of atp. none of the intermediates of glycolysis or pentose phosphate pathway tested could replace atp in the reaction mixture. in absence of exogenously supplied atp, maximum activity was obtained with glu-6-p (68%) followed by fru-6-p (50%) and pep (44%), respectively. with other intermediates as energy sources, the activity ranged from 1 ...19969219433
two herbivores and constraints on selection for resistance in brassica rapa.although most plants experience herbivory by several insect species, there has been little empirical work directed toward understanding plant responses to these simultaneous selection pressures. in an experiment in which herbivory by flea beetles (phyllotreta cruciferae) and diamondback moths (plutella xylostella) was manipulated in a factorial design, i found that selection for resistance to these herbivores is not independent in brassica rapa. specifically, the effect of flea beetle damage on ...199628565704
the morphology, cytology, and c-banded karyotypes of brassica campestris, b. oleracea, and b. napus plants regenerated from protoplasts.the behaviour of brassica campestris (2n=20, aa), b. oleracea (2n=18, cc), and b. napus (2n=38, aacc) were studied during a tissue-culturing process. hypocotyl-protoplasts were cultivated into calli from which new plants were regenerated. the regenerated plants were compared, and mitotic root-tip cells were c-banded and karyotyped. a majority of the plants were tetraploid. the meioses were studied in the pmcs. a number of abberations were observed, mainly due to faulty spindle function. there wa ...199624162299
the slgs corresponding to the same s24-haplotype are perfectly conserved in three different self-incompatible brassica campestris l.we have identified three strains of the same s24-haplotype in self-incompatible b. campestris l. two of them, s-12j and 1-1j, have been derived from japanese populations and one, 27-1t, from turkish one. the cdna clones of slg24 (s24-locus glycoprotein), which linked to the s-locus, were isolated from each strain, and sequenced. each clone isolated from s-12j, 1-1j and 27-1t, was designated as slg24 (s-12j), slg24 (1-1j) and slg24 (27-1t), respectively. their nucleotide sequences were completely ...19968979377
expressed sequence tags of chinese cabbage flower bud cdna.we randomly selected and partially sequenced cdna clones from a library of chinese cabbage (brassica campestris l. ssp. pekinensis) flower bud cdnas. out of 1216 expressed sequence tags (ests), 904 cdna clones were unique or nonredundant. five hundred eighty-eight clones (48.4%) had sequence homology to functionally defined genes at the peptide level. only 5 clones encoded known flower-specific proteins. among the cdnas with no similarity to known protein sequences (628), 184 clones had signific ...19968787028
registration of s alleles in brassica campestris l by the restriction fragment sizes of slgs.polymorphism of slg (the s-locus glycoprotein gene) in brassica campestris was analyzed by pcr-rflp using slg-specific primers. nucleotide sequences of pcr products from 15 s genotypes were determined in order to characterise the exact dna fragment sizes detected in the pcr-rflp analysis. forty-seven lines homozygous for 27 s-alleles were used as plant material. one combination of primers, ps5 + ps 15, which had a nucleotide sequence specific to a class-i slg, gave amplification of a single dna ...199624166262
isolated microspore culture of chinese flowering cabbage (brassica campestris ssp. parachinensis).microspores of several genotypes of brassica campestris ssp. parachinensis have been cultured in vitro and induced to undergo embryogenesis and plant formation. conditions favourable for embryogenesis in this species include a bud size of 2-2.9 mm, nln-13 culture medium (nitsch and nitsch 1967; lichter 1981, 1982; swanson 1990), and an induction through exposure to 32°c for a period of 48 h. longer periods of an elevated temperature for induction of embryogenesis resulted in embryo abortion at e ...199624178416
deduced amino acid sequence of 2s storage protein from brassica species and their conserved structural features.2s seed storage albumin coding regions from five brassica species, namely brassica campestris, b. oleracea, b. nigra, b. juncea, and b. carinata have been cloned by pcr amplification of genomic dna using oligonucleotide primers and their nucleotide sequences have been determined. these sequences showed more than 85% homology amongst themselves and considerable homology with some other crucifer 2s protein coding sequences. the deduced amino acid sequences showed more homology due to some inconseq ...19958714208
molecular cloning of members of the s-multigene family in self-incompatible brassica campestris l.we isolated 12 groups of genomic clones that contained slg-homologous regions from a genomic library constructed from an s9 homozygote of self-incompatible brassica campestris. both slg9 and srk9 genomic clones, which are located within the self-incompatibility (s) locus, were included in these groups. the promoter regions of slg9 and srk9 were completely identical for at least 200 bp upstream from their respective initiation codons (atg). the five sequence elements (boxes i to v) that are conse ...19958564299
immunobiochemical characterization of brassica campestris pollen allergen.brassica campestris (bc), eng. mustard, is an important source of pollen allergen, responsible for type i hypersensitivity disorders. in the present study, bc pollen extract was characterized by tlief, sds-page and immunoprinting. the extract separated into 50 silver stained bands of pi 3-9 on isoelectric focusing whereas it resolved into 14 coomassie blue stained protein bands of 14-100 kd on sds-page. immunoblot analysis with individual patient sera detected four allergenic proteins of 90, 67, ...19957647585
molecular cloning and sequence analysis of a seed-expressed acyl carrier protein (acp) gene from brassica campestris (agrani).we describe here the nucleotide sequence of the brassica campestris acpsf1 gene which encodes a seed-expressed acyl carrier protein (acp). the 3600 bp sequence consists of 1740 bp upstream of the translation start codon, 828 bp spanning the coding region which is interrupted by three introns and 1032 bp downstream of the stop codon. using a acpsf1 gene-specific probe, transcripts could be detected in developing seeds, but not in leaves. the gene is now the only known member that represents group ...19957654784
inheritance of isozymes in brassica campestris l. and genetic divergence among different species of brassiceae.electrophoretic investigations of brassica campestris revealed 8 polymorphic loci that had not been described earlier. the inheritance of 7 of them was determined by crosses between different accessions of b. campestris. a phylogeny of b. campestris, b. nigra, b. alboglabra, sinapis arvensis and s. alba was estimated from the analysis of 15 isozyme loci. the phylogeny showed a close relationship between b. nigra and s. arvensis and a less close relationship between b. campestris and b. oleracea. ...199524169785
genetic variation within and among different cultivars and landraces of brassica campestris l. and b. oleracea l. based on isozymes.genetic variation based on isozymes was studied in 43 landraces and cultivars of brassica campestris from china, 4 cultivars of b. campestris from sweden and 1 from india, and 5 cultivars of b. oleracea from sweden and 1 from china (b. alboglabra). a total of 17 isozyme loci was studied, 10 of these were polymorphic in b. campestris and 6 were polymorphic in b. oleracea. the level of heterozygosity seemed to be reduced in the swedish cultivars compared to the chinese landraces and cultivars of b ...199524169784
the sequences of s-receptor kinases (srk) involved in self-incompatibility and their homologies to s-locus glycoproteins of brassica campestris.s-receptor kinase (srk) is a kind of receptor protein kinase and has a receptor domain resembling s-locus glycoprotein (slg). cdnas encoding srk8 and srk12 were isolated respectively from s8 and s12 haplotypes of brassica campestris. the slg domains of srks were highly homologous to slgs, suggesting that both slg and srk might bind the same ligands in recognition of self-pollen.19957765971
in vitro selection of endosulfan-tolerant strains of brassica compestris (cv. brown sarson).endosulfan tolerant lines of mustard (brassica campestris cv. brown sarson) have been developed through tissue culture methods. cotyledonary expiants excised from eight day old in vitro grown seedlings were used for inducing callus. fast growing friable callus was then transferred to ms medium containing (0.1-2.0 ugl(-1)) endosulfan for selection. five alternating exposures with and without endosulfan containing medium yielded an endosulfan tolerant cell line (etl). the plants regenerated from e ...199424192881
a high degree of homology exists between the protein encoded by slg and the s receptor domain encoded by srk in self-incompatible brassica campestris l.the sporophytic self-incompatibility system in brassica campestris is controlled by multiple alleles of a single locus, s. we isolated and characterized cdna clones that correspond to genes for s-glycoprotein (slg) and s-receptor kinase (srk) from an s9-homozygous strain. an srk9 fragment was amplified by the polymerase chain reaction (pcr) by use of primers that corresponded to regions of the gene for the extracellular receptor domain (s-domain) and the kinase domain. the fragment was used as a ...19947697295
meiotic studies on a brassica campestris-alboglabra monosomic addition line and derived b. campestris primary trisomics.diakinesis chromosomes were studied in pollen mother cells of brassica campestris (2n = 20, genome aa), b. alboglabra (2n = 18, genome cc), a b. campestris-alboglabra monosomic addition line (aa + 1 chromosome from the c genome), and four derived b. campestris primary trisomics. the nucleolar chromosomes of b. campestris were distinguishable by their morphology at diakinesis. the alien c-genome chromosome in the addition line paired preferentially with the nucleolar chromosome of the a genome. v ...199418470102
the sequences of s-glycoproteins involved in self-incompatibility of brassica campestris and their distribution among brassicaceae.s-glycoproteins have been thought to play a role in the self-incompatibility in brassica plants. two cdnas encoding s-glycoproteins were isolated from stigmas of two strains of brassica campestris. their amino acid sequences are highly homologous to each other. however, the numbers of the potential glycosylation sites are different, although most of them are conserved. in the middle part of the sequences, there are two supervariable regions which might contribute to the specificity of each s-gly ...19947764979
role of polyamines on de novo shoot morphogenesis from cotyledons of brassica campestris ssp. pekinensis (lour) olsson in vitro.the promotive effect of ethylene inhibitors (els), i.e. agno3 and aminoethoxyvinylglycine (avg) on de novo shoot regeneration from cultured cotyledonary explants of brassica campestris ssp. pekinensis cv. shantung in relation to polyamines (pas) was investigated. the endogenous levels of free putrescine and spermidine in the explant decreased sharply after 1-3 days of culture, whereas endogenous spermine increased, irrespective of the absence or presence of els. agno3 at 30 μm did not affect end ...199424193830
inhibitory activity of podophyllotoxin and matairesinol-derivative lignans on the root growth of brassica campestris.all the lignans tested in a bioassay with brassica campestris l. subsp. rapa hook. fil. et anders inhibited the root growth of this plant, except for deoxypicropodophyllin. the effects of functional groups in the molecule on the inhibitory activity of these compounds were studied. it is suggested that the methylenedioxyl group and the stereochemical configuration of the lactone junction of podophyllotoxin derivatives were closely related to the inhibitory activity. the o-methyl derivative of two ...199427315720
studies on plant regeneration from cotyledonary protoplasts in brassica campestris.protoplasts isolated from both 7-day-old light-grown and 4-day-old dark/dim light-grown cotyledons of four brassica campestris varieties (arlo, sonja, bunyip and wonk bok) were cultured in three liquid media: modified k8p, modified ms and modified pelletier's b to compare the capacities for cell division and plant regeneration. following cell wall regeneration the cultured protoplasts from dark/dim light-grown cotyledons of four varieties showed rapid division and high frequency of cell division ...199424193644
comparison of processing treatments on the composition and functional properties of rapeseed preparations (brassica campestris l. var. toria).rapeseed preparations viz. rapeseed meal, concentrates and isolates were prepared using different processing treatments involving organic solvents, acids, alkali, steaming and boiling. their anti-nutritional constituents and functional properties were studied in comparison to undefatted meal. percent decrease in phytic acid and phenolic content was maximum in seeds boiled for 30 min and isolates, respectively. isolate ii prepared by sodium hexa-metaphosphate had minimum glucosinolates, maximum c ...19947838214
sequence of a brassica campestris myrosinase gene. 19938278558
bioassembly of acyl lipids in microspore-derived embryos of brassica campestris l.the native lipid composition and the capacity of cell-free extracts to biosynthesize acyl lipids in vitro were determined for the first time using the recently reported microspore-derived (md) embryo system from the brassica campestris low erucic acid line bc-2 (baillie et al. 1992). the total lipid fraction isolated from midcotyledonary stage md embryos (21 days in culture) was composed primarily of triacylglycerol (76%) with an acyl composition quite similar to that of mature bc-2 seed. when i ...199324197336
intergeneric hybridization between diplotaxis siettiana and crop brassicas for the production of alloplasmic lines.intergeneric hybrids were produced between diplotaxis siettiana and brassica campestris through embryo rescue. the hybrids were completely pollen sterile and backcrosses with pollen of b. campestris did not yield any seeds. induction of colchiploidy restored pollen fertility and backcross pollinations yielded viable seeds. cytological details of the hybrid, amphidiploid and backcross progenies were studied. both pollen-sterile and pollen-fertile plants have been obtained in backcross 2 progeny. ...199324196049
characterization of the brassica campestris mitochondrial gene for subunit six of nadh dehydrogenase: nad6 is present in the mitochondrion of a wide range of flowering plants.we have isolated the brassica campestris mitochondrial gene nad6, coding for subunit six of nadh dehydrogenase. the deduced amino-acid sequence of this gene shows considerable similarity to mitochondrially encoded nad6 proteins of other organisms as well as to nad6 proteins coded for by plant chloroplast dnas. the b. campestris nad6 gene appears to lack introns and produces an abundant transcript which is comparable in size to a previously described, unidentified transcript (#18) mapped to the b ...19938431956
the cdna sequence of ns3-glycoprotein from brassica campestris and its homology to related proteins.a cdna clone encoding ns3-glycoprotein was isolated from stigmas of brassica campestris. ns-glycoproteins correspond to the slr1-glycoproteins of b. oleracea and are highly conserved within the species. these data suggest that the ns-glycoproteins may play a role in discrimination between species in the fertilization system of brassica.19938025820
dielectric spectroscopy of plant protoplasts.the relative permittivity and conductivity of the mesophyll protoplasts isolated from brassica campestris leaves and tulipa gesneriana petals were measured over a frequency range from 1khz to 500 mhz.these protoplasts showed a broad dielectric dispersion, which was composed of three subdispersions, termed beta(1)-, beta(2)-, and beta(3)-dispersion in increasing order of frequency.the three subdispersions were assigned to the maxwell-wagner dispersion caused by charging processes at the interface ...199219431864
ecological risks of transgenic plants: effects of spatial dispersion on gene flow.the spread of advantageous transgenic genes from crop plants into wild and weedy relatives is a potential ecological problem. the available theory indicates that the spread of a gene, over space and time, will depend in part on the relative selective advantage of that gene, and in part on gene flow, or the probability distribution of gene movement from source to target plants within a single generation. risk assessment will require knowledge of both. this paper describes an empirical investigati ...199227759266
enhanced shoot regeneration from brassica campestris by silver nitrate.the morphogenetic response of brassica campestris genotype r500 to inhibitors of ethylene biosynthesis and action was investigated. a medium containing 1.0 mg.l(-1) naa, 2.0 mg.l(-1) bap, and 30 or 60 μm agno3 significantly enhanced both the percentage shoot regeneration and the number of shoots per cotyledon expiant. although callus proliferation occurred on hypocotyl segments, no shoots were formed in response to agno3 with expiants older than five days. cotyledons older than six days formed s ...199224213282
agrobacterium-mediated genetic transformation of oilseed brassica campestris: transformation frequency is strongly influenced by the mode of shoot regeneration.protocols were developed for efficient shoot regeneration from hypocotyl and cotyledon explants of oilseed brassica campestris (brown sarson) cv. 'pusa kalyani'. these were used for genetic transformation by an agrobacterium based binary vector carrying neomycin phosphotransferase (npt) gene and β-glucuronidase (gus)-intron gene for plant cell specific expression. transformed plants were recovered from hypocotyl explants at a frequency of 7-13%. addition of silver nitrate markedly enhanced shoot ...199224213158
a brassica campestris-alboglabra addition line and its use for gene mapping, intergenomic gene transfer and generation of trisomics.brassica campestris-alboglabra monosomic addition lines were developed from a trigenomic brassica hybrid (2 n=3 x=29, aac) obtained by backcrossing a resynthesized b. napus (2 n=4 x=38, aacc) line to its parental b. campestris (2 n=2 x=20, aa) line. one addition line was characterized genetically with three loci specific for the alien chromosome and cytologically by meiotic analysis. the following results were obtained. (1) the same chromosome in the b. alboglabra (2 n= 2 x=18, cc) genome carrie ...199224201346
in vitro culture of isolated microspores and regeneration of plants in brassica campestris.a protocol previously developed for b. napus microspore culture was modified to produce embryos from several lines of brassica campestris. bud size, genotype, media constituents, and incubation time and temperature were examined. donor plants were grown in a growth cabinet at a day/night temperature of 10/5°c. microspores were isolated from buds 2.0 - 2.9 mm in length and cultured in modified lichter (1982) medium containing 17% sucrose, ph 6.2. after 48 h at 32°c, the incubation medium was repl ...199224203130
genetic diversity at isozyme and rflp loci in brassica campestris as related to crop type and geographical origin.twenty accessions of brassica campestris, representing the major crop types and their geographical origin, were tested for gene frequency at five isozyme and four rflp loci. the majority of alleles (67%) were found in all geographic regions. nearly 3 times more alleles were detected at rflp loci than at isozyme loci. genetic diversity among crop types (with the exception of turnip) was similar to diversity estimates of geographical regions, implying that crops used for similar purposes (i.e., oi ...199224202754
identification of a novel gibberellin (ga85) in very young seedlings of brassica campestris cv. tobin.a new gibberellin (ga) was identified from extracts of cotyledons of 7 day-old canola seedlings (brassica campestris cv. tobin). this ga is 12α-hydroxy-ga1 and has been assigned the trivial name of ga85. isolation was monitored by the tan-ginbozu dwarf rice micro-drop assay after each high-performance liquid chromatography (hplc) step. identification was based on kovats retention index (kri) and the mass spectrum of the methyl ester, trimethylsilyl ether (metmsi) derivative after analysis by gas ...199227280647
isolation and developmental expression of bcp1, an anther-specific cdna clone in brassica campestris.differential screening of a mature brassica campestris pollen cdna library has identified five cdna clones that represent transcripts expressed exclusively, or at elevated levels, in pollen. we show here that the expression of one of these, clone bcp1, is tissue specific and temporally regulated. the gene is activated during microspore development, as detected by in situ hybridization. expression is enhanced at the time of pollen maturation and during pollen germination. in situ hybridization ha ...19911821760
the cdna sequence of ns1 glycoprotein of brassica campestris and its homology to s-locus-related glycoproteins of b. oleracea. 19911863779
role of ethylene on de novo shoot regeneration from cotyledonary explants of brassica campestris ssp. pekinensis (lour) olsson in vitro.the promotive effect of agno(3) and aminoethoxyvinylglycine (avg) on in vitro shoot regeneration from cotyledons of brassica campestris ssp. pekinensis in relation to endogenous 1-amino-cyclopropane-1-carboxylic acid (acc) synthase, acc, and ethylene production was investigated. agno(3) enhanced acc synthase activity and acc accumulation, which reached a maximum after 3 to 7 days of culture. acc accumulation was concomitant with increased emanation of ethylene which peaked after 14 days. in cont ...199116668148
genetics of brassica campestris. 1. genetic constraints on evolution of life-history allocation arguments suggest a possible tradeoff between timing and magnitude of reproduction: plants that postpone reproduction may accumulate greater resources and consequently produce more offspring. however, early reproduction may be favored when adult mortality is high. tradeoffs among life-history characters may be a consequence of constraints imposed by genetic and environmental covariation among traits. in this paper we examine the genetic basis of the relationship between timing ...199128567862
molecular and cytological evidence of deletions in alien chromosomes for two monosomic addition lines of brassica campestris-oleracea.a series of rflp and isozyme markers were followed in the progenies of two alien addition lines of brassica campestris-oleracea. one of the lines, carrying the c genome chromosome 4 as the alien chromosome, was surveyed for six markers. fifty-four percent of the plants carrying alien chromosomes displayed all the expected makers, whereas the rest had one to five markers missing. the second line for c genome chromosome 5 displayed a similar behavior when surveyed for three markers. all three mark ...199124221206
efficient shoot regeneration of brassica campestris using cotyledon explants cultured in vitro.a system has been developed for efficient regeneration of shoots from brassica campestris in vitro. using 4-day old cotyledons with petioles as expiants and a combination of ba and naa in the regeneration media, up to 70% of expiants produced shoots after 2 weeks in culture. the optimal conditions for regeneration were found to include a ba concentration of 2mgl(-1) and naa concentration of 1mgl(-1). light intensity had a profound effect on regeneration potential. the use of silver ions as an in ...199124220709
leaf physiological aspects of nitrogen-use efficiency in brassica campestris l.: quantitative genetic variation across nutrient treatments.quantitative genetic parameters for leaf physiological and whole-plant aspects of nitrogen-use efficiency in brassica camprestris l. were estimated in three nutrient treatments in the greenhouse. narrow-sense heritabilities and genetic correlations varied across treatments for some traits. sire effects were significant for leaf nitrogen content in near-optimal and super-optimal, but not in suboptimal nutrient treatments. additive genetic variation for two estimates of leaf physiological nitrogen ...199124221160
patterns of mitochondrial dna instability in brassica campestris cultured cells.we previously showed that the mitochondrial dna (mtdna) of a brassica campestris callus culture had undergone extensive rearrangements (i.e. large inversions and a duplication) relative to dna of the control plant [54]. in this study we observed that after continued growth, the mtdna of this culture continues to change, with rearranged forms amplifying and diminishing to varying proportions. strikingly similar changes were detected in the mtdna profiles of a variety of other long- and short-term ...19911653628
effect of agno3 and aminoethoxyvinylglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant brassica genotypes.the presence of 1-10 μm aminoethoxyvinylglycine (avg) or 5-30 μm agno3 markedly enhanced shoot regeneration from cotyledon and hypocotyl cultures of eight recalcitrant brassica campestris and b. juncea genotypes tested. expiants of b. campestris ssp. chinensis and ssp. parachinensis grown with a high avg concentration (20 μm), regenerated poorly. all cytokinins tested were equally effective in promoting shoot formation, except that kinetin was inhibitory to shoot regeneration from hypocotyls of ...199024226701
isolation and characterization of a highly repetitive dna of brassica campestris. 19902102824
effect of cement dust on the growth and yield of brassica campestris l.plants of brassica campestris l. var. g-s20 were treated with cement dust, at rates of 3 (b(1)), 5 (b(2)) and 7 (b(3)) gm(-2) day(-1) for 90 days. treated plants showed a consistent reduction in growth, photosynthetic pigments, yield and oil content over control plants. the overall phytomass of treated plants was significantly decreased, the maximum reduction being 64.8% in b(3) plants, followed by b(2) plants (55.3%) and b(1) plants (43.69%) at 60 days. the effect on oil content was also greate ...199015092252
plant regeneration from isolated microspore cultures of chinese cabbage (brassica campestris spp. pekinensis).isolated microspores of chinese cabbage (brassica campestris ssp. pekinensis) were incubated in modified nn medium containing 10% sucrose in darkness at 33°c for one day followed by culture at 25°c. after 14 days of culture, microspores developed into embryos ranging from globular to cotyledonary stage. plants were regenerated after transfer of embryos to medium containing 3% sucrose and no plant growth regulators.198924233535
oil content and fatty acid composition of promising indian brassica campestris l. (toria) genotypes.seeds of sixty four genotypes of brassica campestris l. (toria) were analyzed for oil content and fatty acid composition. oil content varied from 38.9% to 44.6%. major fatty acids viz. oleic, linoleic, linolenic, eicosenoic and erucic acids exhibited ranges from 10.1% to 17.3%, 5.9% to 14.5%, 5.2% to 15.0%, 7.7% to 13.7% and 39.6% to 59.9%, respectively. compared to the standard cultivar itsa, four genotypes contained 5 to 8% lower content of erucic acid; and six genotypes contained significantl ...19892762244
rearrangement, amplification, and assortment of mitochondrial dna molecules in cultured cells of brassica campestris.we compared brassica campestris mitochondrial and chloroplast dnas from whole plants and from a 2-year-old cell culture. no differences were observed in the chloroplast dnas (cpdnas), whereas the culture mitochondrial dna (mtdna) was extensively altered. hybridization analysis revealed that the alterations are due entirely to rearrangement. at least two inversions and one large duplication are found in the culture mtdna. the duplication element is shown to have the usual properties of a plant mt ...198924232468
the nucleotide sequence of a cdna clone encoding acyl carrier protein (acp) from brassica campestris seeds. 19873658685
extensive mitochondrial specific transcription of the brassica campestris mitochondrial genome.we constructed a complete transcriptional map of the 218 kb brassica campestris (turnip) mitochondrial genome. twenty-four abundant and positionally distinct transcripts larger than 500 nucleotides were identified by northern analyses. approximately 30% (61 kb) of the genome is highly transcribed. in addition, a number of less abundant transcripts, many of which overlap with each other and with the major transcripts, were also detected. if each abundant transcript represents a distinct rrna or p ...19873601669
quantitative cytology of the sperm cells of brassica campestris and b. oleracea.pollen grains of brassica campestris l. var. acephala dc and b. oleracea l. were serially sectioned and examined using transmission electron microscopy to determine the three-dimensional organization of sperm cells within the microgametophyte and the quantity of membrane-bound organelles occurring within each cell. sperm cells occur in pairs within each pollen grain, but are dimorphic, differing in size, morphology and mitochondrial content. the larger of the two sperm cells (svn) is distinguish ...198724233007
photosynthetic carbon fixation characteristics of fruiting structures of brassica campestris l.activities of key enzymes of the calvin cycle and c(4) metabolism, rates of co(2) fixation, and the initial products of photosynthetic (14)co(2) fixation were determined in the podwall, seed coat (fruiting structures), and the subtending leaf (leaf below a receme) of brassica campestris l. cv ;toria.' compared to activities of ribulose-1,5-bisphosphate carboxylase and other calvin cycle enzymes, e.g. nadp-glyceraldehyde-3-phosphate-dehydrogenase and ribulose-5-phosphate kinase, the activities of ...198716665321
isolation of a cdna clone for the acyl carrier protein-i of spinach.a 715 base pair cdna clone coding for an acyl carrier protein (acp) in spinach leaves has been isolated and characterized. the amino acid sequence indicated by the cdna sequence closely matches the amino acid sequence of the acp-i isoform. the presence of polyadenylation and dna sequence coding for a precursor protein with a putative transit peptide, and the absence of hybridization between the cloned dna and isolated spinach plastid dna collectively show that the acp-i gene is nuclear-encoded. ...198724276902
a heat shock protein is encoded within mitochondria of higher plants.a temperature shift from 25 to 41 degrees c initiates the synthesis of a specific set of proteins in maize, including a peptide of 60 kilodaltons. using an in vitro mitochondrial protein synthesizing system, we provide evidence that this 60-kda heat shock protein is encoded within the organelle. further support for this heat-induced protein being encoded within mitochondria is that its synthesis is inhibited in whole seedlings by chloramphenicol. this 60-kda heat shock protein is induced in all ...19854066675
selection of isoresponsive genotypes in toria (brassica campestris l.) based on the pattern of response to environmental variations: a proposed method.thirty-one toria genotypes were compared with three well-established cultivars, 'ludhiana composite-2', 'k-1' and 'tcsu-2' (standard testers). the genotypes, which were almost identical to a standard tester in response to environmental variations and which also had other desirable characteristics, were considered to be acceptable for commercial cultivation. using this criterion, 'tcsu-7', 'th-5' and 'th-4' were found to be acceptable for commercial cultivation. 'th-4' and 'tcsu-7' were found sup ...198524253013
selection of diverse genotypes for heterosis in yield and response in toria (brassica campestris l.).thirty-one genotypes of toria (brassica campestris l.) were grown in twelve environments and subsequently analysed in order to select potential parents which expressed diversity for both 12 different characters (estimated by mahalanobis' d(2) technique) and response to the environments (estimated on the basis of negative correlation between deviations in seed yield of a pair of genotypes from their respective environmental means). coefficients of determination (r(2)) were also used to measure th ...198524254006
isolation and characterization of an iron-containing superoxide dismutase from tomato leaves, lycopersicon esculentum.a cyanide-insensitive superoxide dismutase was purified from tomato leaves (lycopersicon esculentum, mill., var. venture) to apparent homogeneity. the enzyme had twofold higher specific activity (about 4000 standard units) than ferric superoxide dismutases purified from brassica campestris [salin, m. l. and bridges, s. m. (1980) arch. biochem. biophys. 201, 369-374] and nuphar luteum [salin, m.l. and bridges, s. m. (1982) plant physiol. 69, 161-165]. the protein had a relative molecular mass of ...19853967667
on a model for the structure of circular mitochondrial genomes in higher plants.the mitochondrial genome of some higher plants consists of a discrete set of circular molecules. the heterogeneity found in the number of circles and their size is puzzling. in some plants evidence exists that indicates that these circular dna molecules undergo intermolecular and intramolecular recombination events. the result of these events is that two small circles can combine to give one large circle or one large circle can break up into two small circles. here we pursue the idea that such r ...19853870964
[micro-organisms solubilizing insoluble phosphate mineral compounds in the rhizosphere of the rape plant (brassica campestris)].the aim of this study carried out in a soil of the experimental station of the faculty of agronomy of the university of bs. as. was to study the influence of the root of brassica campestris on the functional group of microorganism dissolving insoluble phosphorus mineral compounds, bi and tricalcium phosphate. the microbiological quantitative analysis showed the following values: for bicalcium-phosphate: 5.7 x 10(8), 2.8 x 10(8), and 1.0 x 10(8) and for tricalcium phosphate: 3.2 x 10(8), 1.7 x 10 ...19853870745
genetic divergence among thirty-one genotypes of toria (brassica campestris l.) grown in twelve environments.thirty-one toria genotypes were grown in 12 artificially created environments in order to study genetic divergence. d(2) estimates based on 12 characters were used in obtaining the clustering pattern and inter- and intracluster distances. selection of divergent parents has been made in three ways, i.e., on the basis of (a) genetic divergence exhibited when grown in the richest and most productive environment; (b) stable and consistent values of divergence over all 12 environments and (c) pooled ...198424253702
quantitative cytochemistry of beta-galactosidase in normal and enzyme deficient (gal) pollen of brassica campestris: application of the indigogenic method.the available cytochemical methods for localization of beta-galactosidase have been evaluated using pollen grains of brassica campestris. beta-galactosidase-deficient pollen (gal), served as a control. azo dye methods involving naphthyl substrates showed high and nonspecific background staining to the exine. the indigogenic method, employing 5-bromo-4-chloro-3-indoxyl beta-d-galactoside (x-gal) as the enzyme substrate, gave specific opaque-blue final reaction product, while mutant pollen grains ...19846442287
promotion of flowering in brassica campestris l. cv ceres by sucrose.flower initiation of the quantitative long-day plant brassica campestris cv ceres was earlier and at a lower final leaf number when sucrose was added to the medium in which plants were grown in sterile culture. the optimal concentration of sucrose was 40 to 80 millimolar. this flower-promoting effect of sucrose was not osmotic, as mannitol, sodium chloride, and polyethylene glycol were not effective at equal osmotic potentials.seedlings grown heterotrophically after treatment with 4-chloro-5-(di ...198416663739
a review of the structure and organization of the mitochondrial genome of higher plants.the structure, organisation and functions of the mitochondrial genome of most groups of eukaryotic organisms are known to varying degrees with the notable exception of higher plants. with the recent completion of physical mapping studies on the brassica campestris (chinese cabbage, turnip) and zea mays (maize) mitochondrial genomes, many of the apparent problems of plant mitochondrial genome structure can now be answered. in this manuscript i review the literature relating to the physical observ ...198424310431
plant regeneration in callus and suspension cultures of brassica campestris cv. yellow sarson.prolific shoot bud differentiation was induced in callus and suspension cultures of hypocotyl origin in brassica campestris cv. yellow sarson on ms medium supplemented with k (13.9-23.2 μm) or ba (13.3-22.1 μm). plantlets were obtained by rooting the in vitro differentiated shoots. histological studies revealed a unique mode of meristemoid formation.198424253325
characterization of chloroplasts isolated from triazine-susceptible and triazine-resistant biotypes of brassica campestris l.chloroplasts isolated from triazine-susceptible and triazine-resistant biotypes of brassica campestris l. were analyzed for lipid composition, ultrastructure, and relative quantum requirements of photosynthesis. in general, phospholipids, but not glycolipids in chloroplasts from the triazine-resistant biotype had a higher linolenic acid concentration and lower levels of oleic and linoleic fatty acids, than chloroplasts from triazine-susceptible plants. chloroplasts from the triazine-resistant bi ...198216662454
inheritance of seed colour in turnip rape (brassica campestris l.).the inheritance of seed colour was investigated in the progenies of crosses between seven yellow seeded forms and a brown seeded one of turnip rape (brassica campestris l.). seed colour differences were found to be determined in each case by one or two genes with epistatic effect. moreover, independent inheritance of hilum colour was observed. seed colour was predominantly but not completely determined by the maternal parent. in total, two genes for seed colour with epistatic (br 1 br 1; br 6 br ...198224270566
comparative studies on s-glycoproteins purified from different s-genotypes in self-incompatible brassica species ii. immunological specificities.antisera were prepared by immunization of apparently purified s-glycoproteins; one from an s allele of brassica campestris and two from s alleles of b. oleracea. each antiserum was reactive not only with the homologous s-glycoprotein but also with the heterologous ones, i.e. with the s-glycoproteins of the other s alleles of the same locus. in double diffusion tests, a spur against the heterologous s-glycoproteins suggested heterogeneity of the glycoproteins. the heterogeneity appears to involve ...198217246075
are "multiple cross-multiple pollen hybrids" an answer for productive populations in brassica campestris var. 'brown sarson'? : part 3. potential of component characters in population breeding.results on 167 f1 'mucromphs' reported earlier were re-examined with the view of determining the potential of component characters in correct identification of parental gca status and f1 heterosis. it was found that a judicious combination of 'plant' and 'ratio' characters would be of direct usefulness in assessing population performance. the role of seedling characters in the process of making a desired level of multiple cross was illustrated. it was found that h x l mucromphs could provide a b ...198224271374
is the 'rejection reaction' inducing ability in sporophytic self-incompatible systems restricted only to pollen and tapetum?the present investigation describes the stigmatic rejection response, induced by anther or pollen at developmental stages ranging from anther primordia to mature pollen, as well as in other floral and vegetative tissues. this has been studied in a sporophytic self-incompatible system, brassica campestris (brassicaceae). the implications of these observations have been discussed.198224270697
absence of the iron-containing superoxide dismutase in mitochondria from mustard (brassica campestris).mitochondria were isolated from mature leaves as well as etiolated seedlings of brassica campestris (mustard), a eukaryote previously shown to possess the iron-containing isoenzyme of superoxide dismutase. on the basis of kcn- and h2o2-sensitivity, and on polyacrylamide-gel analysis, only the cuprozinc and mangano superoxide dismutases were found in mitochondria. the iron-containing enzyme was absent. the mangano enzyme was found in the mitochondrial matrix, whereas the cuprozinc enzyme appeared ...19817306048
uniparental inheritance of a chloroplast photosystem ii polypeptide controlling herbicide binding.the ability of atrazine to inhibit photosystem ii electron transport and the rate of electron transfer from the primary to the secondary quinone electron acceptors in the photosystem ii complex were examined in triazine-resistant and -susceptible parental biotypes of brassica campestris l. and their f1 progeny derived from reciprocal crosses. the lack of herbicide inhibitory activity and the presence of functional properties which decreased the q- to b electron transport rate constant were inher ...19817470498
chromosome pairing in haploids of brassica campestris.the maximum chromosome pairing observed in haploids of brassica campestris was two bivalents plus one trivalent but differences were observed in the chromosome pairing frequencies of the four haploids studied. this pairing supports the theorem that the species is hexasomic for one chromosome, tetrasomic for two and disomic for three others but it is emphasized that some of the observed pairing might be explained by a phenomenon other than homology.198124276328
combining ability and maternal effects in brassica campestris l. var. 'yellow sarson'.a diallel analysis of combining ability, including maternal effects, genotype x environment interaction and the progress under selection, is reported in three selected crosses of brassica campestris l. var. 'yellow sarson', involving 15 types, including 10 four-valved and 5 two-valved types from different parts of india. twelve characters, including oil content, were studied in the f1 generation.the investigation has revealed only marginal superiority of f1's over the parents for most of the cha ...198024305915
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