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transformation of brassica oleracea with an s-locus gene from b. campestris changes the self-incompatibility phenotype.an slg gene derived from the s-locus and encoding and s-locus-specific glycoprotein of brassica campestris l. was introduced via agrobacterium-mediated transformation into b. oleracea l. a self-incompatible hybrid and another with partial self-compatibility were used as recipients. the transgenic plants were altered in their pollen-stigma interaction and were fully compatible upon self-pollination. reciprocal crosses between the transgenic plants and untransformed control plants indicated that t ...199124221439
characterization of bcmf23a and bcmf23b, two putative pectin methylesterase genes related to pollen development in brassica campestris ssp. chinensis.two homologous genes, brassica campestris male fertility 23a (bcmf23a) and brassica campestris male fertility 23b (bcmf23b), encoding putative pectin methylesterases (pmes) were isolated from brassica campestris ssp. chinensis (syn. brassica rapa ssp. chinensis). these two genes sharing high sequence identity with each other were highly expressed in the fertile flower buds but silenced in the sterile ones of genic male sterile line system ('bcajh97-01a/b'). results of rt-pcr and in situ hybridiz ...201727844189
pectate lyase-like10 is associated with pollen wall development in brassica campestris.pectate lyase-like10 (pll10) was previously identified as one of the differentially expressed genes both in microspores during the late pollen developmental stages and in pistils during the fertilization process in chinese cabbage (brassica campestris ssp. chinensis). here, antisense-rna was used to study the functions of bcpll10 in chinese cabbage. abnormal pollen was identified in the transgenic lines (bcpll10-4, -5, and -6). in fertilization experiments, fewer seeds were harvested when the an ...201424773757
center of origin and centers of diversity in an ancient crop, brassica rapa (turnip rape).brassica rapa is the most widely distributed and has the longest history of domestication of the agricultural brassica species. molecular genetic diversity, based on 51 simple sequence repeat primer pairs and 715 alleles at polymorphic loci, was used to predict the center of origin and centers of diversity in a global collection of 173 b. rapa accessions. the accessions were separated into 3 molecular genetic groups based on structure analysis-group 1 from the classical old world (europe and wes ...201724714366
instability in mitochondrial membranes in polima cytoplasmic male sterility of brassica rapa ssp. chinensis.cytoplasmic male sterility (cms) is an important factor to observe heterosis in brassica rapa. although several studies have documented the rearrangements of mitochondrial dna and dysfunction in the mitochondria have been observed in most types of cms, the basis of the molecular mechanisms involved in these processes and other effects on cms remain unclear. in this study, suppression subtractive hybridization was performed in the flowers of an alloplasmic polima cms system from b. rapa ssp. chin ...201424652098
microrna expression analysis of rosette and folding leaves in chinese cabbage using high-throughput solexa sequencing.in this study, a global analysis of mirna expression from rosette leaves (rls) and folding leaves (fls) of chinese cabbage (brassica rapa l. ssp. pekinensis) was conducted using high-throughput solexa sequencing. in total, over 12 million clean reads were obtained from each library. sequence analysis identified 64 conserved mirna families in each leaf type and 104 and 95 novel mirnas from rls and fls, respectively. among these, 61 conserved mirnas and 61 novel mirnas were detected in both types ...201324055726
bcmf13, a new reproductive organ-specific gene from brassica rapa. ssp. chinensis, affects pollen development.a transcript-derived fragment (genbank accession number dn237920.1) accumulated in the wild-type flower buds of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) was isolated and further investigated. the full length dna and cdna of the fragment were cloned by rapid amplification of cdna ends. the gene, bcmf13, encodes a protein of 73 amino acids and is interrupted by an intron of 106 bp (genbank accession number ef158459). southern blot analysis reveale ...200817387632
[isolation and characterization of bcmf3, a gene expressed only in maintainer line in chinese cabbage-pak-choi (brassica campestris l. ssp. chinensis makino var. communis tsen et lee)].expression profiling was performed using cdna-aflp technology on floral buds of a genic male sterile line (a line) and a maintainer line (b line) in chinese cabbage pak-choi (brassica campestris l. ssp. chinensis makino var. communis tsen et lee cv. aijiaohuang). a differentially expressed cdna fragment, ba18-t16, was obtained via selective amplification with a18/t16 primer pair. ba18-t16 was specifically associated with the maintainer line, and was expressed only in floral buds revealed by half ...200415651684
trace gases generated in closed plant cultivation systems and their effects on plant growth.interactions between plants and trace gases, especially ethylene, were investigated from two different viewpoints; ethylene is toxic for plant growth, whereas the ethylene release rate of plants can be utilized as a plant growth indicator. when lettuce plants and shiitake mushroom mycelium were cultivated in closed chambers, ethylene concentration increased with time. ethylene was released both from lettuce plant and from shiitake mushroom mycelium. dioctyl phthalate (dop) and dibutyl phthalate ...199511541892
seed predation, pathogen infection and life-history traits in brassica rapa.herbivory and disease can shape the evolution of plant populations, but their joint effects are rarely investigated. families of plants of brassica rapa (brassicaceae) were grown from seeds collected in two naturalized populations in an experimental garden. we examined leaf infection by the fungus alternaria, seed predation by a gall midge (cecidomyiidae) and plant life-history traits. plants from one population had heavier seeds, were more likely to flower, had less fungal infection, had more s ...199528306843
host-associated genetic structure of mexican populations of the cabbage aphid brevicoryne brassicae l. (homoptera: aphididae).phytophagous insects can use different host plant species across their geographic distribution. within a locality, however, their feeding can be restricted to one or two plant species. if host species constitute different selective regimes to herbivorous insects, genetic differentiation and host-associated local adaptation may occur. in this study, we describe the genetic structure of the aphid brevicoryne brassicae l. associated to brassica campestris l. and b. oleraceae var. capitata l., two p ...200314512958
maize grain concentrations and above-ground shoot acquisition of micronutrients as affected by intercropping with turnip, faba bean, chickpea, and soybean.most research on micronutrients in maize has focused on maize grown as a monocrop. the aim of this study was to determine the effects of intercropping on the concentrations of micronutrients in maize grain and their acquisition via the shoot. we conducted field experiments to investigate the effects of intercropping with turnip (brassica campestris l.), faba bean (vicia faba l.), chickpea (cicer arietinum l.), and soybean (glycine max l.) on the iron (fe), manganese (mn), copper (cu) and zinc (z ...201323900569
synthesis and herbicidal activity of novel 3-aminocarbonyl-2-oxazolidinethione derivatives containing a substituted pyridine ring.a series of 3-aminocarbonyl-2-oxazolidinethione derivatives containing a substituted pyridine ring were designed and synthesized. the structures of all of the title compounds were characterized by 1h nmr, 13c nmr, ir, and hrms. their agricultural bioactivities were evaluated, and some of these compounds exhibited good herbicidal activities against echinochloa crusgalli, sorghum vulgare, digitaria sanguinalis, eclipta prostrasta, cucumis sativus, and brassica campestris, which were associated mai ...200616390188
impact and correlation of environmental conditions on pollen counts in karachi, pakistan.a quantitative and qualitative survey of airborne pollen was performed in the city of karachi, and the pollen counts were correlated with different climatic conditions. the aim of the study was to determine the possible effect of meteorological factors on airborne pollen distribution in the atmosphere of karachi city. pollen sampling was carried out by using burkard spore trap for the period of august 2009 to july 2010, and a total of 2,922 pollen grains/m(3) were recorded. in this survey, 22 po ...201525530143
anaerobically digested poultry slaughterhouse wastes as fertiliser in agriculture.chemical and physical analysis, 27-d plant growth assays with carrot (daucus carota) and chinese cabbage (brassica campestris var. chinensis), and 5-d phytotoxicity assays with chinese cabbage and perennial ryegrass (lolium perenne) were used to investigate the suitability of anaerobically digested poultry slaughterhouse waste for fertiliser in agriculture and the effect of aerobic post-treatment on the properties of the digested material. the digested material appeared to be rich in nitrogen. i ...200111265792
a repetitive sequence from diplotaxis erucoides is highly homologous to that of brassica campestris and b. oleracea. 19902103429
design, synthesis, and quantitative structure-activity relationship study of herbicidal analogues of pyrazolo[5,1-d][1,2,3,5]tetrazin-4(3h)ones.a series of pyrazolo[5,1-d][1,2,3,5]tetrazin-4(3h)one derivatives were designed, synthesized, and evaluated for their herbicidal activities where some of these compounds provided >80% control of brassica campestris at 10 microg/ml. quantitative structure-activity relationship studies were performed on these compounds using physicochemical parameters (electronic, verloop, or hydrophobic) as independent parameters and herbicidal activity as a dependent parameter, where herbicidal activity correlat ...200717300154
effects of humic acid on phytodegradation of petroleum hydrocarbons in soil simultaneously contaminated with heavy metals.the use of humic acid (ha) to enhance the efficiency of phytodegradation of petroleum hydrocarbons in soil contaminated with diesel fuel was evaluated in this study. a sample of soil was artificially contaminated with commercially available diesel fuel to an initial total petroleum hydrocarbons (tph) concentration of 2300 mg/kg and four heavy metals with concentrations of 400 mg/kg for pb, 200 mg/kg for cu, 12 mg/kg for cd, and 160 mg/kg for ni. three plant species, brassica campestris, festuca ...201122432335
effect of different fertilizers on nitrogen isotope composition and nitrate content of brassica campestris.the effect of different fertilizers on the δ(15)n value, nitrate concentration, and nitrate reductase activity of brassica campestris and the δ(15)n value of soil has been investigated through a pot experiment. the δ(15)n mean value of b. campestris at the seedling stage observed in the composted chicken treatment (+8.65‰) was higher than that of chemical fertilizer treatment (+5.73‰), compost-chemical fertilizer (+7.53‰), and control check treatment (+7.86‰). there were significantly different ...201222242784
patulibacter brassicae sp. nov., isolated from rhizosphere soil of chinese cabbage (brassica campestris).a novel actinobacterial strain, designated sdt, was isolated from rhizosphere soil of chinese cabbage in shandong province, china. the cells were aerobic, gram-staining-positive, oxidase- and catalase-positive, short rods and formed white colonies on trypticase soy agar. the cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid and alanine, glutamic acid and leucine. diphosphatidylglycerol was the predominant polar lipid. the predominant cellular fatty acid wa ...201627620694
roseomonas soli sp. nov., isolated from an agricultural soil cultivated with chinese cabbage (brassica campestris).a bacterial strain, designated 5n26(t), was isolated from an agricultural soil cultivated with chinese cabbage (brassica campestris). cells of this strain were gram-reaction-negative, strictly aerobic, motile, non-spore-forming rods, and catalase- and urease-negative. the major fatty acids of strain 5n26(t) were c16 : 0 (7.5 %), c18 : 1 2-oh (13.4 %) and summed feature 8 (c18:1ω6c and/or c18:1ω7c; 63.2%). the polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidy ...201424363292
reducing plant uptake of pahs by cationic surfactant-enhanced soil retention.reducing the transfer of contaminants from soils to plants is a promising approach to produce safe agricultural products grown on contaminated soils. in this study, 0-400 mg/kg cetyltrimethylammonium bromide (ctmab) and dodecylpyridinium bromide (ddpb) were separately utilized to enhance the sorption of pahs onto soils, thereby reducing the transfer of pahs from soil to soil solution and subsequently to plants. concentrations of phenanthrene and pyrene in vegetables grown in contaminated soils t ...200919232798
alleviation of heavy metal toxicity and phytostimulation of brassica campestris l. by endophytic mucor sp. mhr-7.heavy metal (hm) pollution is of great concern in countries like pakistan where a huge proportion of human population is exposed to it. these toxic metals are making their way from water bodies to soil where it not only interferes with plant growth and development but also initiates serious health issues in human consuming the produce of such soils. bioremediation is one of the most viable and efficient solution for the problem. purpose of the current study was to isolate endophytic fungi from p ...201728407499
characterization of the small rna transcriptome in plant-microbe (brassica/erwinia) interactions by high-throughput sequencing.non-coding, small rnas (srnas) have been identified in a wide spectrum of organisms ranging from bacteria to humans; however, the role and mechanisms of these srna in plant immunity is largely unknown. to determine possible roles of srna in plant-pathogen interaction, we carried out a high-throughput srna sequencing of brassica campestris using non-infected plants and plants infected with erwinia carotovora. consistent with our hypothesis that distinct classes of host srnas alerts their expressi ...201424126536
composite of pah-degrading endophytic bacteria reduces contamination and health risks caused by pahs in vegetables.vegetables accumulate polycyclic aromatic hydrocarbons (pahs) at high concentrations when grown in contaminated sites. inoculation with pah-degrading endophytic bacteria (ebpah) has been recognized as one of the most promising ways to remove pahs from plant bodies; however, the performance of single endophytic bacteria is generally limited. this investigation used a composite of eight ebpah to reduce the contamination and health risk posed by 16 epa priority pahs in vegetables including chinese ...028448936
bcnrt1, a plasma membrane-localized nitrate transporter from non-heading chinese cabbage.a nitrate transporter, bcnrt1, was isolated from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) cultivar 'suzhouqing'. the full-length cdna was obtained using the rapid amplification of cdna ends technique and contains an open reading frame of 1,770 bp that predicts a protein of 589 acid residues that possesses 12 putative transmembrane domains. using the gus marker gene driven by the bcnrt1 promoter, we found bcnrt1 expression to be concentrated in primary and lateral r ...201222539185
a chinese cabbage (brassica campetris subsp. chinensis) τ-type glutathione-s-transferase stimulates arabidopsis development and primes against abiotic and biotic stress.the beneficial root-colonizing fungus piriformospora indica stimulates root development of chinese cabbage (brassica campestris subsp. chinensis) and this is accompanied by the up-regulation of a τ-class glutathione (gsh)-s-transferase gene (bcgstu) (lee et al. 2011) in the roots. bcgstu expression is further promoted by osmotic (salt and peg) and heat stress. ectopic expression of bcgstu in arabidopsis under the control of the 35s promoter results in the promotion of root and shoot growth as we ...201627796720
pollen selection for alternaria resistance in oilseed brassicas: responses of pollen grains and leaves to a toxin of a. brassicae.the effects of destruxin b, a host-specific toxin of alternaria brassicae that causes black spot disease in oilseed brassicas, were studied on in vitro pollen germination and pollen-tube growth of brassica campestris var "brown sarson", b. juncea, b. napus cvs "westar" and "cresor", b. nigra and sinapis alba. pollen grains of b. nigra, b. juncea and b. campestris were the most sensitive and those of s. alba the least sensitive to the toxin. effects of the toxin were also studied on the leaves of ...199324193480
proteomic analysis of non-heading chinese cabbage infected with hyaloperonospora parasitica.downy mildew is a serious fungal disease in non-heading chinese cabbage (brassica campestris ssp. chinensis makino) that is caused by hyaloperonospora parasitica, which infects members of the brassicaceae family. for breeding improvement, researchers must understand the defence mechanisms employed by non-heading chinese cabbage to combat h. parasitica infection. using 2-de protein analysis, we compared the proteomes from leaves of non-heading chinese cabbage seedlings that were infected with h. ...201424334100
expression and occurrence of uracil-dna glycosylase in higher plants.uracil-dna glycosylase (udg) is the first enzyme in the base excision repair pathway for removal of uracil in dna. dna repair capacity is likely to be a critical factor in mutagenesis and thereby in the capacity to prevent genetic damage and unwanted variation. we have studied expression of udg in 9 higher plant species. the highest expression of udg was measured in solanum tuberosum. a comparison of 6 solanum tuberosum cultivars showed that the specific activity ranged from 30 pmol mg1 protein ...199328741780
brassica compestris var. span: i. fractionation of rapeseed oil by molecular distillation and adsorption chromatography.procedures for the large scale isolation of pure triglycerides and fractions rich in nontriglyceride components from span rapeseed oil are described. fractionation of brassica campestris var. span rapeseed oil by molecular distillation yielded 4 triglyceride fractions, all of which contained traces of sterol esters. an additional triglyceride fraction rich in free and esterified sterols and other volatile components was obtained from the oil. separation by adsorption chromatography of span rapes ...19751177662
generation and identification of brassica alboglabra-brassica campestris monosomic alien addition lines.four monosomic alien addition lines (maals) for brassica alboglabra-brassica campestris were developed through digenomic triploid (acc) backcrossing with the recurrent parent b. alboglabra (cc). the objectives of this study were to compare morphological traits, microsatellite markers (simple sequence repeats), chromosomal karyotypes, and meiotic behaviors. based on the new chromosome nomenclature system established for brassica, we preliminarily identified these maals as cc+a1, cc+a3, cc+a6, and ...201323659701
random amplified polymorphic dna markers of the brassica alboglabra chromosome of a b. campestris-alboglabra addition line.the alien c-genome chromosome in a brassica campestris alboglabra monosomic addition line was characterized by random amplified polymorphic dna (rapd) analysis. the alien chromosome carried three loci, ec, wc and lap-1c, controlling synthesis of erucic acid, white flower colour and a fast-migrating band of leucine aminopeptidase (lap-1cc) respectively. the rapd analysis revealed 17 markers specific to the alien chromosome. among 45 offspring plants from the selfed addition line the alien c-chrom ...19968785604
erratum: mitotic karyotypes of brassica campestris and brassica alboglabra and identification of the b. alboglabra chromosome in an addition line. 199518470209
mitotic karyotypes of brassica campestris and brassica alboglabra and identification of the b. alboglabra chromosome in an addition line.a brassica campestris-alboglabra monosomic addition line (genome: aa + one chromosome from the c genome, 2n = 21) harbours the brassica alboglabra (cc, 2n = 18) chromosome with the gene for erucic acid. in order to identify this chromosome, we have studied the mitotic prometaphase chromosomes of brassica campestris (aa, 2n = 20), b. alboglabra, and the monosomic addition line. more pronounced differential staining and size differences of chromosomes were observed in b. campestris than in b. albo ...199518470170
transcriptomic analysis of pak choi under acute ozone exposure revealed regulatory mechanism against ozone stress.ground-level ozone (o3) is one of the major air pollutants, which cause oxidative injury to plants. the physiological and biochemical mechanisms underlying the responses of plants to o3 stress have been well investigated. however, there are limited reports about the molecular basis of plant responses to o3. in this study, a comparative transcriptomic analysis of pak choi (brassica campestris ssp. chinensis) exposed to different o3 concentrations was conducted for the first time.201729216819
identification and fine mapping of a stay-green gene (brnye1) in pakchoi (brassica campestris l. ssp. chinensis).using bulked segregant analysis combined with next-generation sequencing, we delimited the brnye1 gene responsible for the stay-green trait of nye in pakchoi. sequence analysis identified bra019346 as the candidate gene. "stay-green" refers to a plant trait whereby leaves remain green during senescence. this trait is useful in the cultivation of pakchoi (brassica campestris l. ssp. chinensis), which is marketed as a green leaf product. this study aimed to identify the gene responsible for the st ...201729209732
development of est-ssr markers in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) based on de novo transcriptomic assemblies.flowering chinese cabbage is one of the most important vegetable crops in southern china. genetic improvement of various agronomic traits in this crop is underway to meet high market demand in the region, but the progress is hampered by limited number of molecular markers available in this crop. this study aimed to develop est-ssr markers from transcriptome sequences generated by next-generation sequencing. rna-seq of eight cabbage samples identified 48,975 unigenes. of these unigenes, 23,267 we ...201728902884
isolation and characterization of a phosphorus-solubilizing bacterium from rhizosphere soils and its colonization of chinese cabbage (brassica campestris ssp. chinensis).phosphate-solubilizing bacteria (psb) can promote the dissolution of insoluble phosphorus (p) in soil, enhancing the availability of soluble p. thus, their application can reduce the consumption of fertilizer and aid in sustainable agricultural development. from the rhizosphere of chinese cabbage plants grown in yangling, we isolated a strain of psb (yl6) with a strong ability to dissolve p and showed that this strain promoted the growth of these plants under field conditions. however, systemati ...201728798725
abscisic acid treatment alleviates cadmium toxicity in purple flowering stalk (brassica campestris l. ssp. chinensis var. purpurea hort.) seedlings.the aim of this research was to investigate how exogenous abscisic acid (aba) alleviates cadmium (cd) toxicity in purple flowering stalk (brassica campestris l. ssp. chinensis) and evaluate whether it could be a potential choice for phytoremediation. purple flowering stalk seedlings were cultivated in a hydroponic system with cd at various concentrations (0-100 μmol l-1) as controls and cd plus aba as the treatment in the growth media. the soluble proteins, chlorophyll contents and the activity ...201728755629
elevation of secondary metabolites synthesis in brassica campestris ssp. chinensis l. via exogenous inoculation of piriformospora indica with appropriate fertilizer.this work evaluated the impact of exogenous soil inoculation of beneficial fungal strain piriformospora indica on phytochemical changes and the related genes expression of chinese cabbage (brassica campestris ssp. chinensis l.) by greenhouse pot experiments. high performance liquid chromatography (hplc) affirmed that among the different combinations of fungal and organic fertilizer treatments, the phenolic acids and flavonoids were considerably enriched in organic fertilizer and fungi (op) follo ...201728493970
transcriptomic profiling of two pak choi varieties with contrasting anthocyanin contents provides an insight into structural and regulatory genes in anthocyanin biosynthetic pathway.the accumulation of anthocyanin in horticultural crops not only improves their stress tolerances but also their nutritional values. many key regulatory and structural genes in anthocyanin biosynthesis have been identified in model plants, but limited information is available for non-model plant species featured with colored leaves. in this study, two pak choi varieties with green or purple leaves were selected to analyze the anthocyanin biosynthesis through rna-seq.201728399809
over-expression of mir158 causes pollen abortion in brassica campestris ssp. chinensis.we identified and cloned the two precursors of mir158 and its target gene in brassica campestris ssp. chinensis, which both had high relative expression in the inflorescences. further study revealed that over-expression of mir158 caused reduced pollen varbility, which was caused by the degradation of pollen contents from the binucleate microspore stage. these results first suggest the role of mir158 in pollen development of brassica campestris ssp. chinensis. micrornas (mirnas) play crucial role ...201727909970
the effectiveness of spent coffee grounds and its biochar on the amelioration of heavy metals-contaminated water and soil using chemical and biological assessments.spent coffee grounds (scg) and charred spent coffee grounds (scg-char) have been widely used to adsorb or to amend heavy metals that contaminate water or soil and their success is usually assessed by chemical analysis. in this work, the effects of scg and scg-char on metal-contaminated water and soil were evaluated using chemical and biological assessments; a phytotoxicity test using bok choy (brassica campestris l. ssp. chinensis jusl.) was conducted for the biological assessment. when scg and ...201425242543
expression analysis of self-incompatibility-associated genes in non-heading chinese cabbage.in brassicaceae, a self-incompatibility (si) system mediates pollen-pistil interactions. self-pollen could be recognized and rejected by incompatible pistils. several components involved in the si response have been determined, including s-locus receptor kinase (srk), s-locus cysteine-rich protein/s-locus protein 11, and arm repeat-containing protein 1 (arc1). however, the components involved in the si system of brassicaceae are not fully understood. here, we detected expression patterns of 24 s ...201425062491
molecular cloning and expression of the male sterility-related ctyabby1 gene in flowering chinese cabbage (brassica campestris l. ssp chinensis var. parachinensis).expression of the yabby gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. however, the yabby gene has not been studied in male sterility and fertility restoration. we homologously cloned the ctyabby1 gene of male-sterile tc1 in brassica campestris l. ssp chinensis var. parachinensis; its expression was analyzed by real-time pcr. a 93 ...201425036178
directional transfer of a multiple-allele male sterile line in brassica campestris l. ssp. chinensis (l.) makino var. rosularis tsen et lee.to produce hybrid seeds of wutacai (brassica campestris l. ssp. chinensis (l.) makino var. rosularis tsen et lee), a "directional transfer program" was designed to breed the multiple-allele male sterile line of wutacai. a multiple-allele male sterile line of naibaicai (brassica campestris l. ssp. chinensis l., s01) was used as the male sterile resource, and an inbred line of wutacai (wt01) was used as the target line. recurrent backcrossing was employed to transfer the male sterility and other b ...201424987301
identification of novel and conserved mirnas involved in pollen development in brassica campestris ssp. chinensis by high-throughput sequencing and degradome analysis.micrornas (mirnas) are endogenous, noncoding, small rnas that have essential regulatory functions in plant growth, development, and stress response processes. however, limited information is available about their functions in sexual reproduction of flowering plants. pollen development is an important process in the life cycle of a flowering plant and is a major factor that affects the yield and quality of crop seeds.201424559317
proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions.the physiological and differential proteomic responses of pakchoi leaves and roots to glycine-nitrogen (gly-n) treatments were determined. two pakchoi (brassica campestris ssp. chinensis l. makino. var. communis tsen et lee) cultivars, 'huawang' and 'wuyueman', were grown under sterile hydroponic conditions with different n forms (gly-n and nitrate-n). gly-n-treated pakchoi exhibited decreased fresh weights, total n uptake, leaf areas, and net photosynthetic rates than those treated with nitrate ...201424429133
bcmf21 is important for pollen development and germination in brassica campestris ssp. chinensis.brassica campestris male fertility 21 (bcmf21) was previously isolated from the flower buds of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) and expressed specifically in tapetum and microspores during the meiosis stage and the uninucleate stage of microspore development. here, we used antisense rna technology to knock down the expression level of bcmf21 in b. campestris and analyzed the phenotype of the transgenic plants. alexander staining and scan ...201424323195
the dynamic growth exhibition and accumulation of cadmium of pak choi (brassica campestris l. ssp. chinensis) grown in contaminated soils.the accumulation of heavy metals, especially cadmium (cd), in leafy vegetables was compared with other vegetables. pak choi (brassica campestris l. ssp. chinensis) is a leafy vegetable consumed in taiwan and its safety for consumption after growing in contaminated soils is a public concern. a pot experiment (50 days) was conducted to understand the dynamic accumulation of cd by pak choi grown in artificially contaminated soils. the edible parts of pak choi were sampled and analyzed every 2-3 day ...201324284350
characterization and co-expression analysis of wrky orthologs involved in responses to multiple abiotic stresses in pak-choi (brassica campestris ssp. chinensis).the wrky transcription factor is an important member of the stress-related transcription factors, which mediate diverse abiotic stresses in many plants. however, up until now, the number of wrky members, and the regulatory mechanisms involved in abiotic stress responses in pak-choi (brassica campestris ssp. chinensis), remained unknown.201324267479
comparison of ascorbic acid biosynthesis in different tissues of three non-heading chinese cabbage cultivars.ascorbic acid (l-asa) is an important antioxidant in plants and humans. vegetables are one of the main sources of ascorbic acid for humans. for instance, non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is considered as one of the most important vegetables in south china. to elucidate the mechanism by which asa accumulates, we systematically investigated the expression profiles of d-mannose/l-galactose pathway-related genes. we also investigated the recycling-related genes ...201324157701
identification of micrornas potentially involved in male sterility of brassica campestris ssp. chinensis using microrna array and quantitative rt-pcr assays.micrornas (mirnas) are a class of newly identified, noncoding, small rna molecules that negatively regulate gene expression. many mirnas are reportedly involved in plant growth, development and stress response processes. however, their roles in the sexual reproduction mechanisms in flowering plants remain unknown. pollen development is an important process in the life cycle of a flowering plant, and it is closely related to the yield and quality of crop seeds. this study aimed to identify mirnas ...201323864334
identification of differentially accumulating pistil proteins associated with self-incompatibility of non-heading chinese cabbage.non-heading chinese cabbage (brassica campestris l. ssp. chinensis makino), an important vegetable crop in china, exhibits a typical sporophytic self-incompatibility (si) system. to better understand the mechanism of si response and identify potential candidate proteins involved in the si system of this vegetable crop, the proteomic approach was taken to identify differential accumulating pistil proteins. pistils were collected at 0 h and 2 h after self-pollination at anthesis in self-incompatib ...201423581423
identification of gene expression profile during fertilization in brassica campestris subsp. chinensis.fertilization is controlled by a complex gene regulatory network. to study the fertilization mechanism, we determined time courses of the four developmental stages of fertilization in chinese cabbage pak-choi (brassica campestris subsp. chinensis) by cytological observation. we then used the arabidopsis ath1 microarray to characterize the gene expression profiles of pollinated and unpollinated pistils in b. campestris subsp. chinensis. the result showed 44 up-regulated genes and 33 down-regulate ...201323379337
bcmf11, a novel non-coding rna gene from brassica campestris, is required for pollen development and male fertility.key message : bcmf11 as a non-coding rna gene has an essential role in pollen development, and might be useful for regulating the pollen fertility of crops by antisense rna technology. we previously identified a 828-bp full-length cdna of bcmf11, a novel pollen-specific non-coding mrna-like gene from chinese cabbage (brassica campestris l. ssp. chinensis makino). however, little information is known about the function of bcmf11 in pollen development. to investigate its exact biological roles in ...201323064614
microtubule and male sterility in a gene-cytoplasmic male sterile line of non-heading chinese cabbage.microtubules are the basic components of the cytoskeleton in eukaryotic cells and are made up of 13 parallel protofilaments, each composed of α- and β-tubulin unit molecules aligned along the longitudinal axis of the microtubule.201222581783
sequence characterization and expression pattern of bcmf21, a novel gene related to pollen development in brassica campestris ssp. chinensis.in this report a full length cdna, brassica campestris male fertile 21 (bcmf21) was successfully isolated from one of the cdna-amplified fragment length polymorphism (cdna-aflp) transcript-derived fragments (tdfs), bbp10, that was found down-regulated in the flower buds of sterile plants in brassica campestris l. ssp. chinensis makino genic male sterile (gms) ab line system (bcajh 97-01a/b). bcmf21 protein structure analysis showed a signal peptide at the n-terminus; two protein kinase c phospho ...201222311045
growth promotion of chinese cabbage and arabidopsis by piriformospora indica is not stimulated by mycelium-synthesized auxin.piriformospora indica, an endophytic fungus of the order sebacinales, interacts with the roots of a large variety of plant species. we compared the interaction of this fungus with chinese cabbage (brassica campestris subsp. chinensis) and arabidopsis seedlings. the development of shoots and roots of chinese cabbage seedlings was strongly promoted by p. indica and the fresh weight of the seedlings increased approximately twofold. the strong stimulation of root hair development resulted in a bushy ...201121375386
bcmf20, a putative pollen-specific transcription factor from brassica campestris ssp. chinensis.the arabidopsis transcription factor (at1g26610) was shown to be down-regulated in male sterile lines compared to their maintainer line in chinese cabbage (brassica campestris ssp. chinensis) in our previous study. the bcmf20 gene, homologue of the at1g26610 gene was isolated from chinese cabbage. it encodes a putative c2h2 zinc finger protein. the spatial and temporal expression patterns examined by qrt-pcr and in situ hybridization, indicated bcmf20 is specifically expressed in the developing ...201121327825
isolation and characterization of a novel bcmf14 gene from brassica campestris ssp. chinensis.a putative ralf (rapid alkalinization factor)-like gene (genbank accession number ef523517), named bcmf14, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) by rapid amplification of cdna ends (race) based on a cdna-aflp differential fragment exclusively expressed in fertile line. semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr) discovered that bcmf14 was prominently expressed in stage four and five flower buds ...201120857218
a polygalacturonase inhibitory protein gene (bcmf19) expressed during pollen development in chinese cabbage-pak-choi.the level of polygalacturonase inhibitory protein (pgip) genes involved in pollen development remains unclear. characterization of the different pgip genes that are expressed in pollen is necessary in understanding the similarities and differences of functions between the members of this gene family, as well as the underlying mechanism of pollen development. a gene-encoding putative pgip, bcmf19 was successfully cloned on a cdna-amplified fragment length polymorphism fragment after it was found ...201120373034
differential expression of salt tolerance related genes in brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee.we examined salt tolerance responsive genes in pak-choi under salt stress and analyze their potential function. the mrna differential display was used to screen the transcript derived fragments (tdfs) related to salinity tolerance in tolerant and moderately tolerant pak-choi germplasm. seventy-eight primer combinations generated 101 differential cdna fragments, which were divided into 10 expression types. seven cdna sequences (genbank accession nos. dq006915~dq006921) obtained and sequenced were ...200919882760
the construction of a genetic linkage map of non-heading chinese cabbage (brassica campestris ssp. chinensis makino).non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is one of the most important vegetables in eastern china. a genetic linkage map was constructed using 127 doubled haploid (dh) lines, and the dh population was derived from a commercial hybrid "hanxiao" (lines sw-13 x l-118). out of the 614 polymorphic markers, 43.49% were not assigned to any of the linkage groups(lgs). chi-square tests showed that 42.67% markers were distorted from expected mendelian segregation ratios, and ...200919683673
molecular cloning and characterization of a novel pollen predominantly membrane protein gene bcmf12 from brassica campestris ssp. chinensis.a novel membrane protein gene, bcmf12, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends based on a pollen-specific cdna fragment (dn237936). the cdna was 1,155 bp in length with an open reading frame of 894 bp capable of encoding a putative polypeptide of 297 amino acids with an estimated molecular mass of 34.6 kda and a predicted isoelectric point of 9.6. comparative and bioinformatics analyses revealed that bcmf12 showe ...200919169847
morphological and functional characterization of bcmf13 in the antisense-silenced plants of brassica campestris ssp. chinensis var. parachinensis.the gene brassica campestris male fertility 13 (bcmf13, genbank accession number ef158459) was isolated as a reproductive organ-specific gene from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis). it is exclusively expressed in stage four and five flower buds of fertile lines and is most strongly expressed in stamens. here, we report a functional characterization of this bcmf13 gene in the antisense-silenced plants. the inflorescence of the bcmf13 mutan ...200918592396
transcriptional differences between the male-sterile mutant bcms and wild-type brassica campestris ssp. chinensis reveal genes related to pollen development.a novel male-sterile mutant which lacks mature pollen, brassisa campestris male sterile (bcms), was identified in brassica campestris l. ssp. chinensis makino (syn. b. rapa ssp. chinensis). genetic analysis revealed that bcms was controlled by a single recessive mutation locus. genome-wide transcriptional profiling was performed on the flower buds of both the bcms mutant and the wild-type from which it originated, and profiling analysis indicated that there were numerous changes in gene expressi ...200818426481
functional analysis of a pollen-expressed polygalacturonase gene bcmf6 in chinese cabbage (brassica campestris l. ssp. chinensis makino).in our earlier work, a pollen-expressed polygalacturonase gene bcmf6 was isolated from floral bud of chinese cabbage (brassica campestris l. ssp. chinensis makino) by cdna-amplified fragment length polymorphism (cdna-aflp) transcript profiling and rapid amplification of cdna ends (race). to unravel the biological function of bcmf6 gene, the antisense fragment from the bcmf6 gene with a9 promoter and camv35s promoter was transferred into flowering chinese cabbage (b. campestris ssp. chinensis var ...200818415101
characterization of a male sterile related gene bcmf15 from brassica campestris ssp. chinensis.data from cdna-aflp analysis based on the genome-wide transcriptional profiling on the flower buds of the male meiotic cytokinesis (mmc) mutant and its wild-type of brassica campestris l. ssp. chinensis makino, syn. b. rapa l. ssp. chinensis, indicated that mutation of the mmc gene resulted in changes in expression of a variety of genes. a transcript-derived fragment specifically accumulated in the wild-type flower buds was isolated, and the corresponding full-length cdna and dna was subsequentl ...200918034318
characterization and functional analysis of a novel pcp gene bcmf5 from chinese cabbage (brassica campestris l. ssp. chinensis makino).brassica campestris male fertile 5 (bcmf5), a novel member of the pollen coat protein class a (pcp-a) gene family, was identified from brassica campestris l. ssp. chinensis makino (chinese cabbage-pak-choi). temporal and spatial expression analysis showed that bcmf5 is a late-expressed pcp gene related to the process of determining pollen fertility. functional analysis by hairpin rna (hprna)-mediated rna interference also showed that the expression of bcmf5 is inhibited, which resulted in the lo ...200817945379
[genetic model analysis of leaf-weight traits in non-heading chinese cabbage (brassica campestris ssp. chinensis makino)].the mixed major-gene plus polygene inheritance model was used to analyze the genetics of leafblade-weight and petiole-weight trait in sixqiu017 of non-heading chinese cabbage. the results showed that the leafblade-weight trait was controlled by one pair of negative complete dominant major genes plus additive-dominant polygene (d-4). the additive effect in the major gene was 1.8991, the dominant effect was -1.8991; the additive effect in the polygene was -1.2934, and the dominant effect was 1.793 ...200717855268
identification of flavonoids and hydroxycinnamic acids in pak choi varieties (brassica campestris l. ssp. chinensis var. communis) by hplc-esi-msn and nmr and their quantification by hplc-dad.twenty-eight polyphenols (11 flavonoid derivatives and 17 hydroxycinnamic acid derivatives) were detected in different cultivars of the chinese cabbage pak choi ( brassica campestris l. ssp. chinensis var. communis) by hplc-dad-esi-ms(n). kaempferol was found to be the major flavonoid in pak choi, glycosylated and acylated with different compounds. smaller amounts of isorhamnetin were also detected. a structural determination was carried out by (1)h and (13)c nmr spectroscopy for the main compou ...200717848079
characterization of a putative pollen-specific arabinogalactan protein gene, bcmf8, from brassica campestris ssp. chinensis.the bcmf8 (brassica campestris male fertility 8) gene, possessing the features of 'classical' arabinogalactan protein (agp) was isolated from brassica campestris l. ssp. chinensis, makino syn. b. rapa l. ssp. chinensis. this gene was highly abundant in the fertile flower buds but silenced in the sterile ones of genic male sterile a/b line ('zubajh97-01a/b') in b. campestris. expression patterns analysis suggested bcmf8 was a pollen-specific gene, whose transcript started to be expressed at the u ...200817786584
characterization of a novel gene, bcmf7, that is expressed preferentially in pollen of brassica campestris l. ssp. chinensis makino.pollen formation is important for plant sexual reproduction. to identify the genes that are involved in pollen formation, we performed the genome-wide transcriptional profiling in the flower buds of both male meiotic cytokinesis (mmc) mutant and its wild-type plants of brassica campestris l. ssp. chinensis, syn. b. rapa l. ssp. chinensis. cdna-amplified fragment length polymorphism (cdna-aflp) analysis showed that the mmc mutation resulted in changes in expression of a variety of genes. bcmf7, a ...200717653671
effects of soil properties on heavy metal accumulation in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) in pearl river delta, china.this study was conducted to investigate the effects of soil properties on the heavy metal accumulation in flowering chinese cabbage (brassica campestris l. ssp. chinensis var. utilis tsen et lee) at the field scale. the concentrations of cadmium (cd), mercury (hg), and chromium (cr) in topsoil and vegetable samples from nanhai district of foshan city in the pearl river delta (prd) were analyzed. the results showed that 56.5% of the soil samples exceeded the grade ii of the chinese soil environme ...200717365337
bcmf11, a putative pollen-specific non-coding rna from brassica campestris ssp. chinensis.a full-length cdna, bcmf11, has been cloned from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends (race) based on a pollen-specific cdna fragment (dn237921). the bcmf11 cdna has a total length of 828bp with poly (a) tail. analysis of the sequence demonstrated that bcmf11 is a novel non-coding rna which has no prominent open reading frame (orf) or coding capacity. no significant similarities were observed between bcmf11 and previously publi ...200717207554
[the ultrastructural observation of anthers of chinese cabbage's mail-sterility].the fertile and sterile anthers of a chinese cabbage (brassica campestris l. ssp. chinensis makino) were observed using electron microscope to find the ultrastructural feature of sterile anthers. the earliest abnormal phenomenon in sterile anther was nucleolus of sporogenous cells locating in the edge of nucleus. during microspore mother cell development, callus wall surrounding the cell displayed uneven in the thick ness and was discontinuous,and the some cytoplasm leaked out of the cell from s ...200516416967
[calcium distribution in fertile and sterile anthers of a genic male sterile chinese cabbage].potassium antimonite was used to locate calcium in the fertile and sterile anthers of a genic male sterile chinese cabbage (brassica campestris l. ssp. chinensis makino) to probe the relation between ca(2+) and fertility and sterility of anthers of the cabbage. during fertile anther development, calcium granules increase in number in anther wall cells after meiosis, and then appeared also in locule, suggesting a calcium influx into locule from anther wall cells (plate i-4). then the number of ca ...200516361789
functional analysis of a novel male fertility cyp86mf gene in chinese cabbage (brassica campestris l. ssp. chinensis makino).in our earlier work, a cytochrome p450 cyp86mf gene was isolated from floral bud of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa l.) by mrna differential display pcr (dd-pcr) and rapid amplification of cdna ends (race). to unravel the biological function of cyp86mf gene, the antisense fragment from the cyp86mf gene was transferred into chinese cabbage pak-choi (b. campestris ssp. chinensis var. communis tsen et lee). out of 22 plants transformed with the antisense ...200616075226
role of nitrification inhibitor dmpp (3, 4-dimethylpyrazole phosphate) in no(3-)-n accumulation in greengrocery( brassica campestris l. ssp. chinensis) and vegetable soil.the influence of nitrification inhibitor (ni) 3, 4-dimethylpyrazole phosphate (dmpp) on nitrate accumulation in greengrocery (brassica campestris l. ssp. chinensis) and vegetable soil at surface layer were investigated in field experiments in 2002 and 2003. results showed that ni dmpp took no significant effect on yields of edible parts of greengrocery, but it could significantly decrease no(3-)-n concentration in greengrocery and in vegetable soil at surface layer. in addition, ni dmpp could re ...200515900763
plant wounding affects the oviposition of hellula undalis(f.). the impact of leaf chemistry.crucifer specialists, such as the tropical pest hellula undalis (f.), developed a close association to their hosts and depend on characteristic secondary plant compounds, the glucosinolates (gs), in many cases. following tissue damage, gs undergo hydrolysis catalyzed by the enzyme myrosinase to produce a complex array of products which include isothiocyantes, cyanides, and thiocyanates depending on reaction condition and the chemical nature of the side chain. we found that mechanical plant damag ...200415759429
[the cytochemical observation of anthers of chinese cabbage's male-sterile].a chinese cabbage (brassica campestris l. ssp. chinensis makino) produces 1/4 male sterile and 3/4 fertility in offspring. the sterile plant can be identified from the color of corolla that is some white when it grows out. the fertile and sterile anthers were researched using cytological and cytochemical methods. thick sections of both anthers of different developmental stages were stained with toluidine blue for general cytological observation and stained with the periodic-acid-schiff's (pas) t ...200415511072
genotypic differences in effects of cadmium exposure on plant growth and contents of cadmium and elements in 14 cultivars of bai cai.fourteen cultivars of bai cai (brassica campestris l. ssp. chinensis var. communis) were grown in the nutrient solutions containing 0-0.5 microg ml(-1) of cadmium (cd) to investigate genotypic differences in the effects of cd exposure on the plant growth and uptake and distribution of cd in bai cai plants. the cd exposure significantly reduced the dry and fresh weights of roots and shoots, the dry weight ratio of shoot/root (s/r), total biomass, and chlorophyll content (spad value). cd concentra ...200415473646
[establishment of an efficient regeneration system for chinese cabbage cultured in vitro and histological observation of shoot development].an efficient plant regeneration system from explants of petiole with cotyledon was developed for chinese cabbage (brassica campestris ssp. chinensis makino) in our study. high frequency of shoot regeneration was obtained in the medium containing bap 2 mg/l, naa 0.45 mg/l and 5-7.5 mg/l agno3 solution to the half of nh4+ concentration's ms basic medium, with 84.3 percent being the highest frequency of shoot regeneration in seven chinese cabbage cultivars and one parentage, and the new formed shoo ...200415259989
[reclamation of the plant regeneration efficiency of brassica campestris subsp. chinensis var.parachinensis].this investigation has developed an efficient and fast method for plant regeneration from petiole of cotyledon explants of brassica campestris l. subsp. chinensis makino var. parachinensis tsen et lee. a medium was designed for b. campestris subsp. chinensis var. parachinensis to obtain the high frequency of shoot regeneration, which contained bap 2 mg/l, naa 0.75-1.0 mgl and 7.5 mg/l agno3 solution to the half of nh4+ concentration's ms basic medium. 60 ml/l coconut milk were added to all of me ...200112549111
statistical modeling for estimating glucosinolate content in chinese cabbage by growth conditions.glucosinolate in chinese cabbage (brassica campestris l. ssp. pekinensis (lour.) rupr) has potential benefits for human health, and its content is affected by growth conditions. in this study, we used a statistical model to identify the relationship between glucosinolate content and growth conditions, and to predict glucosinolate content in chinese cabbage.201829315681
the properties and dynamic changes of dom subfractions during food waste and sugarcane leaves co-composting.the purpose of this research was to evaluate the properties and dynamic changes of humic acids (ha), fulvic acids (fa), hydrophobic neutrals (hon), and hydrophilic (hi) fractions of dissolved organic matter (dom) during food waste and sugarcane leaves co-composting process. the pools of ha, fa, hon, and hi were separated from dom by fractionation method, and characterized using spectroscopic (uv-vis, ftir) and pyrolysis-gc/ms analyses. the least peaks in the ha pool were found in ftir spectra wi ...201729280102
chromosomal fragmentation: a possible marker for the selection of high gymnemic acid yielding accessions of gymnema sylvestre r. br.gymnema sylvestre r. br. a member of family asclepiadaceae as mentioned in indian pharmacopoeia popular among the researchers because of stimulatory effect of its phytoconstituent on pancreatic cells and potential to treat type i and ii type of diabetes.201729142402
transcriptomic analysis of the regulation of stalk development in flowering chinese cabbage (brassica campestris) by rna sequencing.flowering chinese cabbage is a stalk vegetable whose quality and yield are directly related to stalk development. however, no comprehensive investigations on stalk development have been performed. to address this issue, the present study used rna sequencing to investigate transcriptional regulation at three key stages (seedling, bolting, and flowering) of stalk development in flowering chinese cabbage. anatomical analysis revealed that cell division was the main mode of stalk thickening and elon ...201729138433
increased biomass and quality and reduced heavy metal accumulation of edible tissues of vegetables in the presence of cd-tolerant and immobilizing bacillus megaterium h3.a cd-resistant and immobilizing bacillus megaterium h3 was characterized for its impact on the biomass and quality and heavy metal uptake of edible tissues of two vegetables (brassica campestris l. var. aijiaohuang and brassica rapa l. var. shanghaiqing) grown in heavy metal-polluted soil. the impact of strain h3 on the soil quality was also evaluated. the increase in the edible tissue biomass and the contents of soluble proteins and vitamin c of the vegetables inoculated with strain h3 ranged f ...201829069614
evaluation of leafy vegetables as bioindicators of gaseous mercury pollution in sewage-irrigated areas.mercury (hg) can evaporate and enter the plants through the stomata of plant leaves, which will cause a serious threat to local food safety and human health. for the risk assessment, this study aimed to investigate the concentration and accumulation of total gaseous mercury (tgm) in five typical leafy vegetables (chinese chives (allium tuberosum rottler), amaranth (amaranthus mangostanus l.), rape (brassica campestris l.), lettuce (lactuca sativa l.), and spinach (spinacia oleracea l.)) grown on ...201829043585
amendment of vanadium-contaminated soil with soil conditioners: a study based on pot experiments with canola plants (brassica campestris l.).we performed pot experiments with canola plants (brassica campestris l.) to evaluate the effect of eight soil conditioners on the amendment of vanadium (v)-contaminated soil based on analysis of the growth of canola plants and the uptake, bioaccumulation, and translocation of heavy metals. tested soil conditioners included polyacrylamide (pam), sepiolite, humic acid (hac), peat, sludge compost (sc), bentonite, lime, and fly ash. results from the analysis of the growth of canola plants and the an ...201729039973
synthesis and biological activity of novel (z)- and (e)-verbenone oxime esters.twenty-seven (z)- and (e)-verbenone derivatives bearing an oxime ester moiety were designed and synthesized in search of novel bioactive molecules. their structures were confirmed by uv-vis, ftir, nmr, esi-ms, and elemental analysis. the antifungal and herbicidal activities of the target compounds were preliminarily evaluated. as a result, compound (e)-4n (r = β-pyridyl) exhibited excellent antifungal activity with growth inhibition percentages of 92.2%, 80.0% and 76.3% against alternaria solani ...201729023419
black plastic mulch combined with summer cover crop increases the yield and water use efficiency of apple tree on the rainfed loess plateau.water deficit significantly limits dryland rainfed fruit production, so increasing water conservation is crucial for improving fruit productivity in arid and semiarid areas. in this study, we tested two treatments in an apple orchard: 1) pc treatment comprising black plastic mulch (bpm) (in-row) with weed control (inter-row); 2) and pgc treatment comprising bpm (in-row) combined with a summer cover crop (inter-row) of rape (brassica campestris l.), which was sown in mid-june and was living from ...201728957428
characterization of lignins isolated with alkali from the hydrothermal or dilute-acid pretreated rapeseed straw during bioethanol production.a better understanding of the lignin in the straw of rapeseed, brassica campestris l., is a prerequisite for promoting the biorefinery industry of rapeseed. two different methods for fractionating lignin from rapeseed straw were proposed in this study. lignin in the raw material was isolated with alkaline solution and recovered by acid precipitation. a comparison between two lignin preparations obtained from two different methods has been made in terms of yield and purity. the structural feature ...201828830775
beneficial effects of tobacco biochar combined with mineral additives on (im)mobilization and (bio)availability of pb, cd, cu and zn from pb/zn smelter contaminated soils.the efficacy of tobacco biochar (tb) alone and in combined with mineral additives: ca-hydroxide (ch), ca-bentonite (cb) and natural zeolite (nz), on immobilization of pb, cd, cu and zn, via reduce its (bio) availability to plants were investigated. the soils were collected from tongguan contaminated (tg-c), fengxian heavily contaminated (fx-hc) and fengxian lightly contaminated (fx-lc) fields, shaanxi province, china. the contaminated top soils were treated with low-cost amendments with an appli ...201728787614
potential use of lime combined with additives on (im)mobilization and phytoavailability of heavy metals from pb/zn smelter contaminated soils.this explorative study was aimed to assess the efficiency of lime alone and in combined with additives to immobilize pb, cd, cu and zn in soil and reduce their phytoavailability for plant. a greenhouse pot experiment was performed by using low and heavily contaminated top soils viz. tongguan contaminated (tg-c); fengxian heavily contaminated (fx-hc) and fengxian low contaminated (fx-lc). the contaminated soils were treated with lime (l) alone and in combined with ca-bentonite (cb), tobacco bioch ...201728756252
uplc-q-exactive orbitrap/ms-based lipidomics approach to characterize lipid extracts from bee pollen and their in vitro anti-inflammatory properties.bee pollen (bp) is collected by honeybees from flower pollen mixed with nectar and its secretions with extensive nutritional and therapeutic properties. lipids are known to be critical contributors for the therapeutic effects of bp and vary depending on different plant sources; however, lipid profiles of bp are not available. here, an uplc-q-exactive orbitrap/ms method was established for comprehensive lipidomics analysis of bp derived from three major nectar plants (brassica campestris l., came ...201728737913
sulfate sorption on rape (brassica campestris l.) straw biochar, loess soil and a biochar-soil mixture.the effects of biochar amendment on sulfur behavior in soils are unknown. in this paper, sulfate (so42-) sorption on rape (brassica campestris l.) straw biochar produced at 600 °c (bc), loess soil (soil) and a 1:9 (w/w) biochar-soil mixture (bc-soil) was investigated by batch experiments. the effects of contact time, initial so42- concentration, temperature and solution ph value on sorption were tested. kinetics, isotherms and thermodynamics for sorption were investigated. pre- and post-sorption ...201728686922
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