| urotensin ii-immunoreactive neurons in the caudal neurosecretory system of freshwater and seawater fish. | antiserum generated against synthetic urotensin ii of the goby, gillichthys mirabilis, was used to localize urotensin ii in the caudal neurosecretory system in six species of freshwater teleosts: cyprinus carpio, carassius auratus, oreochromis mossambicus, oreochromis niloticus, salmo gairdneri and plecoglossus altivelis, and six species of seawater teleosts: acanthogobius flavimanus, pagrus major, parapristipoma trilineatum, trachurus japonicus, seriola dumerili and seriola quinqueradiata. in t ... | 1985 | 3884162 |
| purification and characterization of a tissue-type transglutaminase from red sea bream (pagrus major). | a tissue-type transglutaminase (tgase) was purified from liver tissue of the red sea bream, pagrus major, by ion-exchange chromatography and heparin-sepharose affinity chromatography. its activity was assessed using a fluorometric assay to measure the incorporation of monodansylcadaverine into n,n'-dimethyl casein. the molecular mass of purified tgase was estimated to be 78 kda by sds-polyacrylamide gel electrophoresis. the enzyme required ca2+ to express its activity, although 10 mm sr2+ also a ... | 1994 | 7765597 |
| importation of aquaculture seedlings to japan. | japan is one of the largest consumers of fishery products in the world. importation of various fishery commodities - including larvae and eggs for aquaculture - is increasing due to high consumer demand. elvers of anguilla anguilla have been imported from europe since the late 1960s. eyed eggs of coho salmon (oncorhynchus kisutch) are imported from north america. during the last decade, over-production caused the price of japanese amberjack (or 'yellowtail') (seriola quinqueradiata) and japanese ... | 1996 | 8890372 |
| development of dna diagnostic methods for the detection of new fish iridoviral diseases. | a new disease of epidemic proportions caused by fish viruses within the iridoviridae family inflicts serious damage on red sea breams (pagrus major) and striped jack (caranx delicatissimus) populations grown in aquacultures in japan. a partial segment of the fish iridoviral dna was directly amplified using the polymerase chain reaction (pcr) with synthetic primers designed from well conserved nucleotide sequences between the frog virus 3 (ranavirus) and the silkworm iridescent virus type 6. the ... | 1997 | 9094219 |
| characterization of copper/zinc-superoxide dismutase from pagrus major cdna and enzyme stability. | a full-length cdna of 794 bp encoding a putative copper/zinc-superoxide dismutase (cu/zn-sod) from pagrus major was cloned by the pcr approach. nucleotide sequence analysis of this cdna clone revealed that it comprises a complete open reading frame coding for 154 amino acid residues. the deduced amino acid sequence showed high similarity (53-91%) with the sequences of cu/zn-sod from other species. computer analysis of the residues required for coordinating copper (his-47, 49, 64, and 121) and zi ... | 2002 | 11829645 |
| in vitro metabolism of fenthion and fenthion sulfoxide by liver preparations of sea bream, goldfish, and rats. | the in vitro metabolism of fenthion and its sulfoxide (fenthion sulfoxide) in sea bream (pagrus major) and goldfish (carassius auratus) was investigated and compared with that in rats. fenthion was oxidized to fenthion sulfoxide and the oxon derivative, but not to its sulfone, in the presence of nadph by liver microsomes of sea bream, goldfish, and rats. these liver microsomal activities of the fish were lower than those of rats but were of the same order of magnitude. the nadph-linked oxon- and ... | 2003 | 12527698 |
| studies on epizootic iridovirus infection among red sea bream, pagrus major (temminck & schlegel), cultured in taiwan. | since 1993, an epizootic viral disease has occurred in net-cage cultured red sea bream, pagrus major (temminck & schlegel), in peng-hu island located on the south-western coast of taiwan. the diseased fish exhibited abnormal swimming and were lethargic, but few visible external signs were observed. the cumulative mortality because of the disease sometimes reached 50-90% over 2 months. histopathogical studies of the affected fish showed enlarged basophilic cells in the gill, kidney, heart, liver ... | 2003 | 12962222 |
| susceptibility of cultured juveniles of several marine fish to the sevenband grouper nervous necrosis virus. | piscine nodaviruses (betanodaviruses) have been tentatively divided into four genotypes (sjnnv, rgnnv, tpnnv and bfnnv) and it is suggested that host specificity is different among these genotypes. in the present study, a betanodavirus [sevenband grouper nervous necrosis virus (sgnnv)] belonging to the redspotted grouper nervous necrosis virus (rgnnv) genotype, to which most betanodaviruses from warm water fish are identified, was evaluated for its pathogenicity to hatchery-reared juveniles of s ... | 2003 | 12962219 |
| purification and characterization of three isoforms of chrysophsin, a novel antimicrobial peptide in the gills of the red sea bream, chrysophrys major. | we report here the isolation of three isoforms of a novel c-terminally amidated peptide from the gills of red sea bream, chrysophrys (pagrus) major. peptide sequences were determined by a combination of edman degradation, ms and hplc analysis of native and synthetic peptides. three peptides, named chrysophsin-1, chrysophsin-2, and chrysophsin-3, consist of 25, 25, and 20 amino acids, respectively, and are highly cationic, containing an unusual c-terminal rrrh sequence. the alpha-helical structur ... | 2003 | 12581207 |
| metabolism of 2-nitrofluorene, an environmental pollutant, by liver preparations of sea bream, pagrus major. | 1. the in vitro metabolism of 2-nitrofluorene (nf), an environmental pollutant, was examined in fish, focusing on nitro-reduction followed by n-acylation and hydroxylation. 2. when nf was incubated with liver microsomes or cytosol of sea bream, pagrus major, in the presence of nadph or 2-hydroxypyrimidine, 2-aminofluorene (af) was formed. 3. when af was incubated with liver cytosol in the presence of acetyl-coa or n-formyl-l-kynurenine, 2-acetylaminofluorene (aaf) or 2-formylaminofluorene (faf) ... | 2002 | 12296988 |
| effect of dietary fatty acids on lipoprotein lipase gene expression in the liver and visceral adipose tissue of fed and starved red sea bream pagrus major. | juvenile red sea bream pagrus major were fed either a commercial diet (diet 1) or diets supplemented with 10% oleate (diet 2), 5% oleate+5% linoleate (diet 3) or 5% oleate+5% n-3 polyunsaturated fatty acid mixture (diet 4) for 4 weeks. following the conditioning period, the effects of dietary fatty acids on lipoprotein lipase (lpl) gene expression in the liver and visceral adipose tissue of fed (5 h post-feeding) and starved (48 h post-feeding) fish were investigated by competitive polymerase ch ... | 2002 | 12095871 |
| comparison of substrate specificities of transglutaminases using synthetic peptides as acyl donors. | transglutaminase (tgase) is an enzyme that catalyzes acyl transfer reactions between primary amines and gln residues in proteins or peptides. substrate specificities of tgase, ca2+-independent microbial transglutaminase (mtgase), and ca2+-dependent tissue type transglutaminase from guinea pig liver (gtgase) and fish, red sea bream (pagrus major), liver (ftgase), for acyl donors were investigated using synthetic peptides containing gln residues and gln analogues with different lengths of side cha ... | 2000 | 11210124 |
| purification, characterization, and molecular cloning of group i phospholipases a2 from the gills of the red sea bream, pagrus major. | phospholipase a2 (pla2) activity was investigated in various tissues of male and female red sea bream. in both male and female fishes, the specific activity of pla2 in the gills was 70 times higher than that in other tissues, such as the adipose tissue, intestine, and hepatopancreas. therefore, we tried to purify pla2 from the gill filaments of red sea bream to near homogeneity by sequential chromatography on q-sepharose fast flow, butyl-cellulofine, and deae-sepharose fast flow columns, and by ... | 2000 | 11201998 |
| lympholeukemia in madai pagrus major in japan. | lympholeukemia has been occurring to an epizootic extent with mass mortality in 1 and 2 yr old madai (= japanese red sea bream) pagrus major in the winter season (october-may) in the western regions of japan since 1975. diseased fish displayed severe anemia and markedly increased numbers of neoplastic lymphocytoid and lymphoblastoid cells in the blood. neoplastic cells originated in the splenic lymphatic cells and systemically caused severe metastatic lesions in the heart, liver, kidney, digesti ... | 2000 | 10782349 |
| sequence and expression of a cdna encoding the red seabream androgen receptor. | the cdna of the androgen receptor (ar) has been isolated from the ovary of red seabream, pagrus major, and sequenced. the amino acid sequence of red seabream ar (rsar) shows about 45% identity with those of xenopus, rat, mouse, and human ars. it is shown that rsar has the ability to trans-activate the responsive gene depending on the presence of androgen. | 1999 | 10395960 |
| effectiveness of a vaccine against red sea bream iridoviral disease in a field trial test. | since 1990, red sea bream iridovirus (rsiv) has caused high mortalities in the summertime in cultured red sea bream pagrus major in southwest japan. to establish control measures for red sea bream iridoviral disease (rsivd), the effectiveness of a formalin-killed viral vaccine was evaluated in a field trial. two groups each consisting of 1000 juvenile red sea bream were either intraperitoneally inoculated with vaccine (vaccinated group) or were not vaccinated (non-vaccinated group). after vaccin ... | 1999 | 10349554 |
| distribution and seasonal variations in levels of three native gnrhs in the brain and pituitary of perciform fish. | specific and sensitive radioimmunoassays (rias) were newly developed for two types of gonadotropin-releasing hormone (gnrh), namely, seabream (sb) gnrh and chicken (c) gnrh-ii. we employed these two rias together with a previously reported ria for salmon (s) gnrh to study the presence and regional distribution of these three gnrhs in the brains and pituitaries of four perciform fishes (red seabream, pagrus major; black seabream, acanthopagrus schlegeli; striped knifejaw, oplegnathus fasciatus; a ... | 1999 | 10201813 |
| sequence and expression of a cdna encoding the red sea bream androgen receptor. | the cdna of the androgen receptor (ar) has been isolated from the ovary of red sea bream, pagrus major, and sequenced. the amino acid sequence of red sea bream ar (rsar) shows about 45% identity with that of xenopus, rat, mouse, and human ar. it is shown that rsar has the ability to trans-activate the responsive gene depending on the presence of androgen. | 1999 | 10082979 |
| overproduction of dnaj in escherichia coli improves in vivo solubility of the recombinant fish-derived transglutaminase. | the overexpression of red sea bream (pagrus major) transglutaminase (tgase, e.c. 2.3.2.13) in escherichia coli mostly leads to the accumulation of biologically inactive enzyme. although the solubility of the gene products could be improved by cultivation at a lower temperature (26-28 degrees c), most of the synthesized tgase was still in the form of insoluble aggregates. the effects of overproduction of molecular chaperones on the intracellular solubility of newly produced recombinant tgase were ... | 1998 | 9692205 |
| purification of nadph-linked and nadh-linked quinone reductases from liver cytosol of sea bream, pagrus major. | quinone reductases in sea bream, pagrus major, were investigated using menadione as a model quinone. both nadph-linked and nadh-linked quinone reductase activities were detected, in varying degrees, in all tissues examined. in the liver, these activities resided in its microsomal and cytosolic fractions. the cytosolic activity was markedly inhibited by cupric sulfate and p-chloromercuribenzoate. however, little effect was observed with dicoumarol, a potent inhibitor of dt-diaphorase. the nadh-li ... | 1997 | 9467879 |
| base changes in the flic gene of edwardsiella tarda: possible effects on flagellation and motility. | edwardsiella tarda is a broad host-range pathogen infecting both animals and humans. e. tarda isolates from red sea bream pagrus major are non-motile, whereas isolates from japanese eel anguilla japonica and japanese flounder paralichthys olivaceus are motile with peritrichous flagella. we compared the flic gene coding for flagellin (flic) in motile and non-motile e. tarda strains isolated from diseased fish. twenty-two amino acid residues differed in the predicted flic amino acid sequences betw ... | 2007 | 17760384 |
| regulation of vibrio alginolyticus virulence by the luxs quorum-sensing system. | quorum sensing (qs) is a bacterial intercommunication system that controls the expression of multiple genes in response to population density. the luxs qs system regulates the expression of several virulence factors in a wide variety of pathogenic bacteria. luxs has been characterized to be responsible for producing a type of autoinducer, ai-2, which stimulates the expression of the luciferase operon in vibrio harveyi. vibrio alginolyticus is established as an opportunistic pathogen of several m ... | 2008 | 18261029 |
| cross-reactivity of anti-yellowtail thymic lymphocyte monoclonal antibody (yet-2) with lymphocytes from other fish species. | the monoclonal antibody yet-2, generated in mice hyper-immunized with thymic lymphocytes of the yellowtail, seriola quinqueradiata, reacts with the major population of peripheral blood lymphocytes, which might be putative t cells. in this study, we examined the cross-reactivity of yet-2 with lymphocytes from various fish species. flow cytometric analysis showed that yet-2 reacts with 69.8% lymphocytes in the thymus, 89.7% in the peripheral blood, 87.5% in the spleen, and 59.7% in the head-kidney ... | 1997 | 9161694 |
| insulin and insulin-like growth factors i and ii induce final maturation of oocytes of red seabream, pagrus major, in vitro. | the effects of various growth factors and peptides on in vitro germinal vesicle breakdown (gvbd) were examined in oocytes of the red seabream, pagrus major, a daily spawning marine teleost. the oocytes used underwent gvbd in response to hcg but not to 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (dhp). insulin (1 microm) and insulin-like growth factor-i (igf-i, 10 nm) and igf-ii (13 nm) induced gvbd. salmon gonadotropin-releasing hormone (gnrh), chicken gnrh (100 nm), angiotensin ii and iii (10 mi ... | 1994 | 7958758 |
| molecular cloning of a cdna encoding the prepro-salmon gonadotropin-releasing hormone of the red seabream. | complementary dna fragments encoding the prepro-salmon gonadotropin-releasing hormone ([trp7, leu8]gnrh, sgnrh) of the red seabream pagrus major were amplified from mrna of the olfactory bulbs using a reverse transcriptase-polymerase chain reaction (rt-pcr), and the full-length cdna was cloned from a cdna library using the pcr-amplified cdna as a probe. the cdna consisted of 442 bp, including an open reading frame of 270 bp which encoded the prepro-sgnrh (90 amino acid residues). the prepro-sgnr ... | 1994 | 7851723 |
| tissue-type transglutaminase from red sea bream (pagrus major). sequence analysis of the cdna and functional expression in escherichia coli. | a cdna clone encoding a tissue-type transglutaminase (tgase) was isolated from a cdna library prepared from the liver of red sea bream (pagrus major). the cdna sequence had an open reading frame coding for a protein of 695 amino acids and showed 43% identity to the sequence of guinea pig liver tgase, revealing a relatively low overall similarity. however, the 25-amino-acid sequence containing the putative active site (cys272) of the enzyme was completely conserved between the two species, and wa ... | 1995 | 7556189 |
| development of maturational competence of oocytes of red seabream, pagrus major, after human chorionic gonadotropin treatment in vitro requires rna and protein synthesis. | the effects of human chorionic gonadotropin (hcg) and 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (dhp) on in vitro germinal vesicle breakdown (gvbd) in oocytes obtained at different times of day from a daily spawning marine teleost, the red seabream pagrus major, were investigated. oocytes isolated at 0800 hr underwent gvbd in response to hcg (10 iu/ml) but not to dhp (10 ng/ml). gvbd could be induced in oocytes isolated at 1600 hr by either hcg or dhp. oocytes underwent gvbd in response to dhp ... | 1994 | 7523239 |
| growth-promoting activity of tuna growth hormone and expression of tuna growth hormone cdna in escherichia coli. | tuna (thunnus thynnus) growth hormone (gh) was purified by using a column of sepharose 4b to which tuna gh-specific igg was linked. the molecular weight and isoelectric point of tuna gh were 21,000 and 6.5, respectively. the growth of snapper (pagrus major) was remarkably accelerated when the purified hormone was administered by four intraperitonial injections at intervals of 5 days: 1.5-fold in length and 1.9-fold in body weight/60 days. to produce tuna gh in escherichia coli cells, expression ... | 1988 | 3064767 |
| establishment and characterization of a fibroblast cell line derived from the dorsal fin of red sea bream, pagrus major (temminck & schlegel). | the establishment and partial characterization of a continuous cell line from the dorsal fin of red sea bream, pagrus major, are described. the cell line, designated rsbf-2, has been subcultured for more than 100 passages since its initiation in november 2000. it was optimally maintained at 28 degrees c in leibovitz l-15 medium with 10% foetal bovine serum. propagation of rsbf-2 cells was serum dependent and exhibited low plating efficiency (<1.7%). aside from long-term cryopreservation, the cel ... | 2010 | 20102463 |
| attenuation of sound by schooling fish. | attenuation of sound by schooling fish was measured or estimated for yellowtail (seriola quinqueradiata), sea bream (pagrus major), and spotted mackerel (scomber australasicus) in culture nets and a net cage. the results, along with those of other researchers, are normalized to obtain extinction cross sections per 2/3 power of the body weight. in the normalized cross sections there are no large differences among fish species, sizes, and measuring methods. the attenuation increases slightly with ... | 1992 | 1506535 |
| observations on a cucullanid nematode of marine fishes from taiwan strait, dichelyne (cucullanellus) jialaris n. sp. | during a helminthological examination of marine fishes from south of the minnan-taiwan bank fishing ground, taiwan strait, fujian, china, a new cucullanid nematode, dichelyne (cucullanellus) jialaris n. sp., was removed from the intestine of the red seabream, pagrus major (temminck & schlegel, 1834). the new species differs from its congeners mainly in the following characters: body size medium but with relative long spicules of 1.01 mm (0.97-1.06) in length or 20.0% (18.21-21.8%) of the body le ... | 2004 | 15270107 |
| protection of red sea bream pagrus major against red sea bream iridovirus infection by vaccination with a recombinant viral protein. | megalocytivirus infections cause serious mass mortality in marine fish in east and southeast asian countries. in this study the immunogenicity of crude subunit vaccines against infection by the megalocytivirus rsiv was investigated. three capsid proteins, 18r, 351r and a major capsid protein, were selected for use as crude subunit vaccines. high homology among megalocytivirus types was found in the initial sequence examined, the 351r region. red sea bream (pagrus major) juveniles were vaccinated ... | 2010 | 20236423 |
| ontogenic development of three gnrh systems in the brain of a pleuronectiform fish, barfin flounder. | a pleuronectiform fish, the barfin flounder verasper moseri, has three molecular forms of gonadotropin-releasing hormone (gnrh) in the brain, salmon gnrh (sgnrh), chicken gnrh-ii (cgnrh-ii) and seabream gnrh (sbgnrh). to elucidate the ontogenic origin of the neurons that produce these gnrh molecules, the development of three gnrh systems was examined by in situ hybridization and immunocytochemistry. neuronal somata that express sgnrh mrna were detected first in the vicinity of the olfactory epit ... | 2004 | 15056926 |
| two species of parasitic copepods (caligidae) new to japan. | two species of parasitic copepods (siphonostomatoida: caligidae), caligus sclerotinosus roubal, armitage & rohde, 1983, parasitic on red seabream pagrus major (temminck & schlegel), and lepeophtheirus longiventralis yü & wu, 1932, parasitic on spotted halibut verasper variegatus (temminck & schlegel), are redescribed based on material found on their respective hosts cultured in japan. both species are new to japan. preliminary observation on the occurrence of l. longiventralis indicates that the ... | 2004 | 14739672 |
| [cloning and expression of tumor necrosis factor (tnfalpha) cdna from red seabream pagrus major]. | a fragment of tnfalpha cdna sequence from red seabream was cloned by homology cloning approach with two degenerated primers which were designed based on the conserved regions of other animals' tnf sequences. the sequence was elongated by 3' and 5' race to get the full length cds sequence. this sequence contained 1264 nucleotides that included a 5' utr of 85 bp, a 3' utr of 514 bp and an open reading frame (orf) of 666 bp which could encode 222 amino acids propeptide. in 3' utr, there were severa ... | 2003 | 14673503 |
| mucus ph of the tiger puffer takifugu rubripes is an important factor for host identification by the monogenean heterobothrium okamotoi. | we examined a host-finding factor of the monogenean heterobothrium okamotoi oncomiracidia to develop an alternative prophylaxis. h. okamotoi oncomiracidia attached preferentially to gill filaments and skin mucus from the tiger puffer takifugu rubripes compared with corresponding material from other tested fishes (amber jack seriola dumerili, red sea bream pagrus major, japanese flounder paralichthys olivaceus and spotted halibut verasper variegatus). the body mucus ph of the tiger puffer was 6.4 ... | 2003 | 12964825 |
| cloning and characterisation of natural resistance associated macrophage protein (nramp) cdna from red sea bream (pagrus major). | nramp (natural resistance associated macrophage protein) controls aspects of innate resistance to intracellular parasites. its function is to enhance the ability of macrophages to kill pathogens. however, little is known about the structure and function of nramp in lower vertebrates such as teleosts. we have recently isolated a cdna encoding nramp from spleen of red sea bream (pagrus major). the full-length cdna of the nramp is 4709 bp in length, including 197 bp 5'-terminal untranslated region ... | 2004 | 15312657 |
| molecular cloning and characterization of alpha-class glutathione s-transferase genes from the hepatopancreas of red sea bream, pagrus major. | two distinct cdnas corresponding to gsta1 and gsta2 genes encoding glutathione s-transferases (gsts) from the hepatopancreas of red sea bream, pagrus major were cloned and sequenced. a comparison of the nucleotide sequences of gsta1 and gsta2 revealed 98% identity and their derived amino acid sequences had 96% similarity. both genes could be classified as alpha-class gsts on the basis of their amino acid sequence identity with other species. genomic dna cloning showed that both gsta1 and gsta2 g ... | 2005 | 15946636 |
| the glycosphingolipid receptor for vibrio trachuri in the red sea bream intestine is a gm4 ganglioside which contains 2-hydroxy fatty acids. | three major glycosphingolipids (tentatively designated igl-1, 2, and 3) were isolated from the intestine of red sea bream (pagrus major) and were subjected to a tlc-overlay assay with (35)s-labeled vibrio trachuri which causes vibriosis of fish. the bacteria adhered to igl-2, which was determined to be a gm4 ganglioside (neuacalpha2-3galbeta1-ceramide). the fatty acid portion of igl-2 was composed of 2-hydroxy c22:0, c24:0, and c24:1, in addition to the non-hydroxy c16:0 and c18:0, while the sph ... | 2005 | 15979459 |
| megalocytiviruses. | the genus megalocytivirus, represented by red sea bream iridovirus (rsiv), the first identified and one of the best characterized megalocytiviruses, infectious spleen and kidney necrosis virus (isknv), the type species of the genus, and numerous other isolates, is the newest genus within the family iridoviridae. viruses within this genus are causative agents of severe disease accompanied by high mortality in multiple species of marine and freshwater fish. to date outbreaks of megalocytivirus-ind ... | 2012 | 22590684 |
| henneguya pagri n. sp. (myxozoa: myxosporea) causing cardiac henneguyosis in red sea bream, pagrus major (temminck & schlegel). | henneguya pagri n. sp. (myxozoa: myxosporea) is described from netpen-cultured red sea bream, pagrus major, in japan. affected fish displayed anaemic gills, an enlarged bulbus arteriosus and internal haemorrhaging in the pericardial cavity. disease outbreaks occurred during the summer and the prevalence of infection reached 75% in august. plasmodia of h. pagri developed in the bulbus arteriosus of red sea bream, in which degenerative cardiomyopathy was observed. following maturation of the paras ... | 2005 | 16159366 |
| immunogenicity, retention and protective effects of the protein derivatives of formalin-inactivated red seabream iridovirus (rsiv) vaccine in red seabream, pagrus major. | a formalin-inactivated virus was previously found to be efficient in protecting fish against challenge with red seabream iridovirus (rsiv), a dna virus belonging to the iridoviridae family. in the present study, we determined the amount of the virus in the vaccine in terms of the number of copies of the gene for the major capsid protein (mcp) gene by quantitative real-time pcr and examined the longevity and types of immune response generated after intramuscular vaccination. we also tested whethe ... | 2006 | 16213752 |
| [red sea bream iridoviral disease]. | the first outbreak of red sea bream iridoviral disease caused by red sea bream iridovirus (rsiv) was recorded in cultured red sea bream pagrus major in shikoku island, japan in 1990. since 1991, the disease has caused mass mortalities of cultured marine fishes not only red sea bream but also many other species. the affected fish were lethargic and exhibited severe anemia, petechiae of the gills, and enlargement of the spleen. the causative agent was a large, icosahedral, cytoplasmic dna virus cl ... | 2005 | 16308538 |
| kudoa iwatai (myxosporea: multivalvulida) in wild and cultured fish in the red sea: redescription and molecular phylogeny. | gilt-head sea bream, sparus aurata l., the mediterranean's most important mariculture species, has been cultured for the last 30 yr in eilat (israeli red sea). kudoa sp. was the first myxosporean parasite reported from this species. in recent years, an increase in prevalence in both land-based and sea-cage facilities in eilat has been observed. infections with the same kudoa species appeared in cultured european sea bass dicentrarchus labrax (l.) and grey mullet mugil cephalus in the same farms, ... | 2005 | 16419765 |
| effects of insulin, triiodothyronine and fat soluble vitamins on adipocyte differentiation and lpl gene expression in the stromal-vascular cells of red sea bream, pagrus major. | various kinds of hormones including insulin, triiodothyronine (t(3)) and fat-soluble vitamins have been proposed as mediators of adipocyte differentiation in mammals. to investigate the factors which are responsible for fish adipocyte differentiation, we developed a serum-free culture system of stromal-vascular cells of red sea bream adipose tissue and examined the effects of bovine insulin, t(3), and fat-soluble vitamins (all-trans retinoic acid, retinyl acetate and 1,25-dihydroxyvitamin d(3)) ... | 2006 | 16716627 |
| molecular phylogenetic relationships of china seas groupers based on cytochrome b gene fragment sequences. | the classification and evolutionary relationships are important issues in the study of the groupers. cytochrome b gene fragment of twenty-eight grouper species within six genera of subfamily epinephelinae was amplified using pcr techniques and the sequences were analyzed to derive the phylogenetic relationships of the groupers from the china seas. genetic information indexes, including kimura-2 parameter genetic distance and ts/tv ratios, were generated by using a variety of biology softwares. w ... | 2006 | 16856492 |
| application of the arming system for the expression of the 380r antigen from red sea bream iridovirus (rsiv) on the surface of yeast cells: a first step for the development of an oral vaccine. | the cell surface is a functional interface between the inside and the outside of the cell. moreover, cells have systems for anchoring surface specific proteins and for confining surface proteins to particular domains on the cell surface. for use in bioindustrial processes applied to oral vaccination, we consider that cell-surface display systems must be useful and that the yeast saccharomyces cerevisiae, the most suitable microorganism for practical purposes, is available as a host for genetic e ... | 2006 | 16889368 |
| involvement of host recognition by oncomiracidia and post-larval survivability in the host specificity of heterobothrium okamotoi (monogenea: diclidophoridae). | heterobothrium okamotoi, a monogenean gill parasite, shows high host specificity for tiger puffer, takifugu rubripes. in the present study, in vivo and in vitro experimental infections were conducted using various fish species, including t. rubripes, to understand the mechanisms of specificity. in in vivo experiments, t. rubripes, grass puffer, takifugu niphobles, olive flounder, paralichthys olivaceus, and red sea bream, pagrus major, were exposed to oncomiracidia of h. okamotoi labelled with a ... | 2007 | 17055510 |
| characterization of a new fibroblast cell line from a tail fin of red sea bream, pagrus major, and phylogenetic relationships of a recent rsiv isolate in japan. | red sea bream iridovirus (rsiv) is a causative agent of red sea bream iridoviral disease (rsivd) in marine fish species in japan. fibroblast cells were developed from a tail fin of red sea bream, pagrus major, and then underwent single cell cloning. the successful cloned cells were named crf-1 cells. most crf-1 cells had a normal diploid karyotype with 2n=48 by chromosomal analysis. rsiv-infected crf-1 cells showed typical morphological changes that were associated with apoptosis by egfp-annexin ... | 2007 | 17335926 |
| taxonomical study into two new species of philometra (nematoda: philometridae) previously identified as philometra lateolabracis (yamaguti, 1935). | males of the nematode philometra lateolabracis (yamaguti, 1935), the type species of the genus philometra costa, 1845, were discovered for the first time in gonads of its type host, the japanese seaperch, lateolabraxjaponicus (cuvier). morphological comparisons carried out between the collected male and female p. lateolabracis with the male and female philometrid nematodes previously reported as p. lateolabracis infecting chicken grunt, parapristipoma trilineatum (thunberg), and red sea bream, p ... | 2008 | 18578165 |
| leucine aminopeptidase from red sea bream (pagrus major) skeletal muscle: purification, characterization, cellular location, and tissue distribution. | a leucine aminopeptidase was purified for the first time from marine fish red sea bream ( pagrus major) skeletal muscle to homogeneity with 4850-fold and a yield of 7.4%. the purification procedure consisted of ammonium sulfate fractionation and chromatographies including deae-sephacel, sephacryl s-200, hydroxyapatite, and phenyl-sepharose. the enzyme was approximately 96 kda as estimated by sds-page and gel filtration and preferentially hydrolyzed substrate leu-mca. the enzymatic activity was o ... | 2008 | 18800800 |
| cadmium toxicity to embryonic-larval development and survival in red sea bream pagrus major. | at 18 degrees c and 33 psu, 24 and 48 h lc(50) values of cadmium (cd) for red sea bream pagrus major embryos were 9.8 and 6.6 mgl(-1), respectively, while 24, 48, 72, and 96 h lc(50) values for larvae were 18.9, 16.2, 8.0, and 5.6 mgl(-1), respectively, indicating that embryos were more sensitive to cd toxicity than larvae. cd concentrations at > or =0.8 mgl(-1) led to low hatchability (0-90% in > or =0.8 mgl(-1) solutions vs. 97-100% in lower ones), delay in time to hatch, high mortality (38-10 ... | 2009 | 19573918 |
| identification of two distinct types of beta-2 microglobulin in marine fish, pagrus major and seriola quinqueradiata. | two types of beta-2 microglobulin (beta2m) were identified in red seabream and yellowtail from expressed sequence tag (est) analyses of kidney and spleen cells. a cluster of 21 ests from red seabream showed high homology to beta2m from other teleosts, while another cluster containing 7 ests was also homologous to beta2m. similarly, two clusters containing 21 and 2 ests were identified to be homologous to beta2m in yellowtail. the genes in the large and small clusters of each fish were designated ... | 2010 | 19819561 |
| purification and characterization of a collagenolytic serine proteinase from the skeletal muscle of red sea bream (pagrus major). | a collagenolytic serine proteinase (csp) was purified from red sea bream (pagrus major) skeletal muscle to homogeneity by ammonium sulfate fractionation and chromatographies including deae-sephacel, phenyl sepharose and hydroxyapatite. the molecular mass of csp was approximately 85 kda as estimated by sds-page and gel filtration. optimum temperature and ph of csp were 40 degrees c and 8.0, respectively. csp was specifically inhibited by serine proteinase inhibitors, while inhibitors to other typ ... | 2010 | 19945542 |
| toxicity of metal pyrithione photodegradation products to marine organisms with indirect evidence for their presence in seawater. | we evaluated the acute toxicities of the metal pyrithiones (mepts)--copper pyrithione (cupt) and zinc pyrithione (znpt)--to four species of marine algae and a marine crustacean (tigriopus japonicus). we also performed acute toxicity tests using six of the main mept photodegradation products: pyridine-n-oxide (po); 2-mercaptopyridine (hps); pyridine-2-sulfonic-acid (psa); 2-mercaptopyridine-n-oxide (hpt); 2,2'-dithio-bis-pyridine ([ps](2)); and 2,2'-dithio-bis-pyridine-n-oxide ([pt](2))-and three ... | 2010 | 19967345 |
| effect of long-term cryopreservation on physiological characteristics, antioxidant activities and lipid peroxidation of red seabream (pagrus major) sperm. | the aim of this study was to determine the effect of long-term cryopreservation on physiological characteristics, the antioxidant activities and lipid peroxidation of red seabream sperm which were respectively cryopreserved with 15% dimethylsulfoxide (me(2)so) for 1 month, 13 months, 26 months, 48 months and 73 months. the motility and fertility of post-thaw sperm decreased with the storage time going on. the highest motility (87.67±2.52%) was obtained in sperm cryopreserved for 1 month and the ... | 2010 | 20654607 |
| exposure of eggs to solar uv-b leads to reduced hatching rates in two sparid fishes, red sea bream pagrus major and black sea bream acanthopagrus schlegeli. | hatching success was examined under exposure to solar ultraviolet radiation (uvr) using filters to give three different light conditions [c1: uv-b, uv-a and photosynthetically active radiation (par), c2: uv-a and par, c3: par] in red pagrus major and black acanthopagrus schlegeli sea bream. hatching rate of both species was reduced by an exposure over a 2 day period to uvr and was not significantly different between two species under the three light conditions. | 2010 | 20666911 |
| antimicrobial resistance of vibrio parahaemolyticus and vibrio alginolyticus strains isolated from farmed fish in korea from 2005 through 2007. | the antimicrobial resistance patterns to 15 antimicrobial agents of vibrio parahaemolyticus and vibrio alginolyticus isolated from farmed fishes, including olive flounder (paralichthys olivaceus), black rockfish (sebastes schlegeli), red sea bream (pagrus major), and sea bass (lateolabrax japonicus), were investigated from 2005 through 2007. a total of 218 v. parahaemolyticus isolates and 153 v. alginolyticus isolates were obtained from the 180 fish samples collected from fish farms located alon ... | 2011 | 21375873 |
| toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on the peripheral nervous system of developing red seabream (pagrus major). | we investigated 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd)-induced effects on the morphology of peripheral nervous system (pns) in the developing red seabream (pagrus major) embryos. the embryos at 10h post-fertilization (hpf) were treated with 0, 0.1, 0.4 or 1.7 μg/l of tcdd in seawater for 80 min. the morphology of pns was microscopically observed with florescence staining using an anti-acetylated tubulin antibody at 48, 78, 120 and 136 hpf. axon length of facial nerve (vii) was found to be sh ... | 2012 | 23314332 |
| isolation and identification of bile salts conjugated with cysteinolic acid from bile of the red seabream, pagrosomus major. | bile salts present in gallbladder of wild and cultured red seabream, pagrosomus major, a marine teleost were analyzed. the bile from wild red seabream was found to contain two previously unknown bile salts along with two known bile salts, taurocholate and taurochenodeoxycholate. isolation of each bile salt was performed by column chromatography. fast atom bombardment mass spectra of the unknown bile salts showed the molecular ions (m-h)- of m/z 544 and 528 which are shifted 30 mass units upfield ... | 2006 | 1797942 |
| a new class of glutathione s-transferase from the hepatopancreas of the red sea bream pagrus major. | to elucidate drug deposition and metabolism in cultured marine fishes, in a previous study we isolated and purified the gsts (glutathione s-transferases) from the hepatopancreas of the red sea bream pagrus major that contained 25 and 28 kda gst subunits. the 25 kda gst subunits encoded by two genes (gsta1 and gsta2) have been identified as alpha-class gsts. in the present study, we performed the molecular cloning and characterization of the gstr1 gene encoding the 28 kda gst subunit from the pa. ... | 0 | 15610066 |
| cytological studies on induced mitogynogenesis in japanese flounder paralichthys olivaceus (temminck et schlegel). | the effect of hydrostatic pressure treatment on the induction of mitogynogenesis in the eggs of japanese flounder paralichthys olivaceus (temminck et schlegel) by using heterospecific sperm were studied. before treatment, the eggs were at metaphase of the first mitosis. the spindle was disassembled by the treatment and then resembled in its pretreatment position, and the chromosomes were rearranged, i.e., the first mitosis was not blocked. during the second mitotic cycle, only a monopolar spindl ... | 2016 | 26796308 |
| monogeneans (platyhelminthes) from marine fishes of tongyeong, korea. | monogeneans (platyhelminthes) mostly parasitize on fins, skin and gills of fishes. in korea, the study on monogeneans is limited, although, fishes are frequently encountered with severe infection of monogeneans. hence, some of ranched and wild fishes were collected from tongyeong marine living resources research and conservation center, southern part of korea to screen and understand the infection of monogeneans. all three fish hosts were found with the infection of monogeneans including five sp ... | 2014 | 25035585 |
| short-term starvation and realimentation helps stave off edwardsiella tarda infection in red sea bream (pagrus major). | dietary regime modifications have been an integral part of health and healing practices throughout the animal kingdom. thus, to assess the effects of periodic starvation and refeeding schedule on the physiological and immunological perturbations in edwardsiella tarda infected red sea bream, we conducted a 20day experiment using 4 treatment groups, namely, pre-fed placebo (pfp); pre-starved placebo (psp); pre-fed infected (pfi); and pre-starved infected (psi), wherein a 5h e. tarda infection was ... | 2017 | 28163184 |
| starvation beneficially influences the liver physiology and nutrient metabolism in edwardsiella tarda infected red sea bream (pagrus major). | dietary compromises, especially food restrictions, possess species-specific effects on the health status and infection control in several organisms, including fish. to understand the starvation-mediated physiological responses in edwardsiella tarda infected red sea bream, especially in the liver, we performed a 20-day starvation experiment using 4 treatment (2 fed and 2 starved) groups, namely, fed-placebo, starved-placebo, fed-infected, and starved-infected, wherein bacterial exposure was done ... | 2015 | 26188170 |
| effects of protein hydrolysates supplementation in low fish meal diets on growth performance, innate immunity and disease resistance of red sea bream pagrus major. | this study was conducted to evaluate the supplemental effects of three different types of protein hydrolysates in a low fish meal (fm) diet on growth performance, feed utilization, intestinal morphology, innate immunity and disease resistance of juvenile red sea bream. a fm-based diet was used as a high fish meal diet (hfm) and a low fish meal (lfm) diet was prepared by replacing 50% of fm by soy protein concentrate. three other diets were prepared by supplementing shrimp, tilapia or krill hydro ... | 2015 | 26074096 |
| draft genome sequences of two genetic variant strains of edwardsiella piscicida, jf1305 and rsb1309, isolated from olive flounder (paralichythys olivaceus) and red sea bream (pagrus major) cultured in japan, respectively. | edwardsiella piscicida is a new species discovered within the group of organisms traditionally classified as edwardsiella tarda. we present draft genome sequences of two variant strains of e. piscicida, jf1305 and rsb1309. differences in protein-coding sequence between these isolates are associated with virulence, disease, and defense, suggesting differences in pathogenicity. | 2014 | 24926054 |
| immune responses and stress resistance in red sea bream, pagrus major, after oral administration of heat-killed lactobacillus plantarum and vitamin c. | the present study evaluated the interactive benefits of dietary administration of heat-killed lactobacillus plantarum (lp) and vitamin c (vc) on the growth, oxidative status and immune response of red sea bream (pagrus major). a diet without lp and vc supplements was employed as a control diet. four other test diets with 0 or 1 g lp kg(-1) combined with 0.5 or 1 g vc kg(-1) (2 × 2 factorial design) were fed to red sea bream (2 ± 0.01 g) for 56 days. a significant interaction was found between lp ... | 2016 | 27095173 |
| interaction effects of dietary supplementation of heat-killed lactobacillus plantarum and β-glucan on growth performance, digestibility and immune response of juvenile red sea bream, pagrus major. | both heat-killed lactobacillus plantarum (hk-lp) and β-glucan (bg) play important roles in growth performance, feed utilization and health status of fish. therefore, a feeding trial was conducted to determine the interactive effects of dietary hk-lp and bg on growth performance, digestibility, oxidative status and immune response of red sea bream for 56 days. a significant interaction was found between hk-lp and bg on final body weight, total plasma protein, glucose, serum bactericidal activity ... | 2015 | 25661844 |
| biochemical characteristics of four marine fish skins in korea. | in this study, we investigated the biochemical characteristics of the fish skins of four industrial species: olive flounder (paralichthys olivaceus), black rockfish (sebastes schlegeli), sea bass (lateolabrax maculatus) and red sea bream (pagrus major). there is high domestic demand in korea for farming of these fish for human consumption. crude protein contents in the skin of these fish ranged from 73% to 94% by dry weight; this was in part due to a high content of the structural protein, colla ... | 2014 | 24767045 |
| probiotics as an environment-friendly approach to enhance red sea bream, pagrus major growth, immune response and oxidative status. | a usual strategy in modern aquaculture to combat production bottlenecks associated with intensification is preventive health care through the use of consumer and environment-friendly alternatives including probiotics. the current study evaluates the influence of lactobacillus rhamnosus (lr), a lyophilized probiotic bacterium, on health status and performance of red sea bream (pagrus major). probiotics were incorporated in the diets at four different concentrations: 0 (control diet, lr0), 10(2) ( ... | 2016 | 27542618 |
| physiological response, blood chemistry profile and mucus secretion of red sea bream (pagrus major) fed diets supplemented with lactobacillus rhamnosus under low salinity stress. | environmental stressors caused by inadequate aquaculture management strategies suppress the immune response of fish and make them more susceptible to diseases. therefore, efforts have been made to relieve stress in fish by using various functional feed additives in the diet, including probiotics. the present work evaluates the effects of lactobacillus rhamnosus (lr) on physiological stress response, blood chemistry and mucus secretion of red sea bream (pagrus major) under low salinity stress. fi ... | 2017 | 27542150 |
| effects of dietary supplementation of lactobacillus rhamnosus or/and lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, pagrus major. | pagrus major fingerlings (3·29 ± 0·02 g) were fed with basal diet (control) supplemented with lactobacillus rhamnosus (lr), lactococcus lactis (ll), and l. rhamnosus + l. lactis (lr + ll) at 10(6) cell g(-1) feed for 56 days. feeding a mixture of lr and ll significantly increased feed utilization (fer and per), intestine lactic acid bacteria (lab) count, plasma total protein, alternative complement pathway (acp), peroxidase, and mucus secretion compared with the other groups (p < 0.05). serum ly ... | 2016 | 26766177 |
| genetic vaccines protect red seabream, pagrus major, upon challenge with red seabream iridovirus (rsiv). | we have investigated the protective effect of immunization of juvenile red seabream, pagrus major, with dna plasmids encoding the viral major capsid protein (mcp) and an open reading frame (orf) containing a transmembrane domain against red seabream iridovirus (rsiv). the expression of the mhc class i transcript in the dna-vaccinated fish was significantly upregulated at the 15th day post-vaccination and the relative level of expression was maintained until the 30th day post-vaccination. this pa ... | 2006 | 16359871 |
| rapid detection of a fish iridovirus using loop-mediated isothermal amplification (lamp). | a loop-mediated isothermal amplification (lamp) procedure is described to detect the genomic dna molecule of red seabream iridovirus (rsiv), a fish iridovirus belonging to the iridoviridae family. the rsiv dna was amplified using dna extracts obtained from spleen of infected red seabream, pagrus major and from various rsiv isolates. the method was at least 10 times more sensitive than conventional pcr in detecting for the presence of rsiv. a striking feature of the lamp reaction is its ability t ... | 2004 | 15381352 |
| vibrios adhere to epithelial cells in the intestinal tract of red sea bream, pagrus major, utilizing gm4 as an attachment site. | vibrios, distributed in marine and brackish environments, can cause vibriosis in fish and shellfish under appropriate conditions. previously, we clarified by thin-layer chromatography (tlc) overlay assay that (35)s-labeled vibrio trachuri adhered to gm4 isolated from red sea bream intestine. however, whether gm4 actually functions on epithelial cells as an attachment site for vibrios still remains to be uncovered. we found that six isolates, classified as v. harveyi, v. campbellii, and v. splend ... | 2013 | 23320941 |
| erratum to: effect of taurine supplementation on hepatic metabolism and alleviation of cadmium toxicity and bioaccumulation in a marine teleost, red sea bream, pagrus major. | | 2017 | 28078494 |
| trace elements in two marine fish cultured in fish cages in fujian province, china. | two cultured marine fish, the japanese seabass (lateolabrax japonicus) and red seabream (pagrus major) were collected from eight fish cage sites along the coast of fujian province in china. the concentrations of ag, as, cd, co, cu, fe, mn, se, and zn in their muscle, stomach and liver tissue were quantified. the risk of these trace elements to humans through fish consumption was then assessed. the highest concentrations of as, cd, se and zn in fish feed from fish cages were found in dongshan sta ... | 2010 | 20149944 |
| effects of cooking and subcellular distribution on the bioaccessibility of trace elements in two marine fish species. | in current human health risk assessment, the maximum acceptable concentrations of contaminants in food are mostly based on the total concentrations. however, the total concentration of contaminants may not always reflect the available amount. bioaccessibility determination is thus required to improve the risk assessment of contaminants. this study used an in vitro digestion model to assess the bioaccessibility of several trace elements (as, cd, cu, fe, se, and zn) in the muscles of two farmed ma ... | 2010 | 20192204 |
| effect of taurine supplementation on hepatic metabolism and alleviation of cadmium toxicity and bioaccumulation in a marine teleost, red sea bream, pagrus major. | this study was performed to unravel the mechanism of the beneficial action of taurine on marine teleost fish, red sea bream (pagrus major), by analyzing the hepatic metabolism. moreover, the ameliorative effects of the nutrient against cadmium toxicity and bioaccumulation were further evaluated. the fish were fed a diet containing 0 % (tau0 %), 0.5 % (tau0.5 %), or 5.0 % (tau5.0 %) taurine for 40-55 days (d) and subjected to cadmium acute toxicity and bioaccumulation tests. taurine deficiency in ... | 2017 | 27535560 |
| cloning and expression analysis of innate immune genes from red sea bream to assess different susceptibility to megalocytivirus infection. | as suggested by the office international des epizooties (oie), fishes belonging to the genus oplegnathus are more sensitive to megalocytivirus infection than other fish species including red sea bream (pagrus major). to assess the roles of the innate immune response to these different susceptibilities, we cloned the genes encoding inflammatory factors including il-8 and cox-2, and the antiviral factor like mx from red sea bream for the first time and performed phylogenetic and structural analysi ... | 2017 | 27523084 |
| dietary effects of adenosine monophosphate to enhance growth, digestibility, innate immune responses and stress resistance of juvenile red sea bream, pagrus major. | our study explored the dietary effects of adenosine monophosphate (amp) to enhance growth, digestibility, innate immune responses and stress resistance of juvenile red sea bream. a semi-purified basal diet supplemented with 0% (control), 0.1% (amp-0.1), 0.2% (amp-0.2), 0.4% (amp-0.4) and 0.8% (amp-0.8) purified amp to formulate five experimental diets. each diet was randomly allocated to triplicate groups of fish (mean initial weight 3.4 g) for 56 days. the results indicated that dietary amp sup ... | 2016 | 27514786 |
| effects of brown seaweed polyphenols, α-tocopherol, and ascorbic acid on protein oxidation and textural properties of fish mince (pagrosomus major) during frozen storage. | frozen storage of minced fish is currently one of the most important techniques to maintain its functional properties. however, some deterioration does occur during frozen storage and cause quality loss. the effects of brown seaweed polyphenols, α-tocopherol, and ascorbic acid on lipid and protein oxidation and textural properties of red sea bream (pagrosomus major) during 90 days of frozen storage at -18 °c were investigated. | 2017 | 27282659 |
| comparative developmental toxicity of eight typical organic pollutants to red sea bream (pagrosomus major) embryos and larvae. | the red sea bream (pagrosomus major) 48-h embryo-larval bioassay was used to assess and compare the developmental toxicities of eight typical organic pollutants, including polycyclic aromatic hydrocarbons (pahs), organophosphorus pesticides, polychlorinated biphenyls (pcbs) and alkylphenol. toxicological endpoints such as survival rates of p. major embryos or larvae and the rates of hatching and of malformation (oedema, condensed blood, spinal curvatures or eye abnormalities) were noted and desc ... | 2016 | 27130341 |
| molecular characterization of muscle-parasitizing didymozoid from a chub mackerel, scomber japonicus. | didymozoids found in the muscles of marine fish are almost always damaged because they are usually found after being sliced. therefore, identifying muscle-parasitizing didymozoids is difficult because of the difficulty in collecting non-damaged worms and observing their organs as key points for morphological identification. moreover, muscle-parasitizing didymozoids are not easily found because they parasitize at the trunk muscles. therefore, muscle-parasitizing didymozoid classification has not ... | 2015 | 26204013 |
| application of a solid-phase fluorescence immunoassay to determine amoxicillin residues in fish tissue. | the present study demonstrates an application of parallux (a solid-phase fluorescence immunoassay) for amoxicillin analysis in fish tissue. amoxicillin at the recommended therapeutic dose (400 mg/kg body weight) was orally administered to three groups of 25 olive flounder ( paralichthys olivaceus ), 25 rockfish ( sebastes schlegeli ) and 25 red sea bream ( pagrus major ) for 7 consecutive days. amoxicillin was detected in the muscle of fish treated by the 3rd day of the withdrawal period. the re ... | 2010 | 20159742 |
| the complete mitochondrial genome of the hybrid of pagrus major (♀) × acanthopagrus schlegelii (♂). | the complete mitochondrial sequence of the hybrid from red bream (pagrus major ♀) × black porgy (acanthopagrus schlegelii ♂) was firstly determined by using the pcr-based method. the total length of the mitogenome genes of the hybrid was 17 028 bp in accordance with red bream, and it contained 13 protein-coding genes, 22 transfer rna genes, two ribosomal rna genes and one control regions. compared with the complete mitochondrial genome of its parents, the results showed that the hybrid of p. maj ... | 2016 | 26122335 |
| effects of large-scale releases on the genetic structure of red sea bream (pagrus major, temminck et schlegel) populations in japan. | large-scale hatchery releases are carried out for many marine fish species worldwide; nevertheless, the long-term effects of this practice on the genetic structure of natural populations remains unclear. the lack of knowledge is especially evident when independent stock enhancement programs are conducted simultaneously on the same species at different geographical locations, as occurs with red sea bream (pagrus major, temminck et schlegel) in japan. in this study, we examined the putative effect ... | 2015 | 25993089 |
| oxidative stress, neurotoxicity, and non-specific immune responses in juvenile red sea bream, pagrus major, exposed to different waterborne selenium concentrations. | juvenile pagrus major (mean length 15.8±1.6 cm, and mean weight 90.4±4.7 g) were exposed for 4 weeks with waterborne selenium concentration (0, 50, 100, 200, and 400 μg l(-1)). in oxidative stress indicators, liver and gill superoxide dismutase (sod) activity and glutathione s-transferase (gst) activity were markedly elevated after 4 weeks exposure. similarly, glutathione (gsh) level in liver and gill was also increased in response to the highest se exposure after 4 weeks exposure. in neurotoxic ... | 2015 | 25898389 |
| a new species of glugea thélohan, 1891 in the red sea bream pagrus major (temminck & schlegel) (teleostei: sparidae) from china. | a new microsporidian species is described from farmed red sea bream pagrus major (temminck & schlegel) (teleostei: sparidae). large numbers of spherical whitish xenomas were observed throughout the visceral organs of the host. histological examination showed that the microsporidia caused several xenomas that were embedded in the intestinal muscularis externa or submucosa. light and transmission electron microscopy examination of the spores also revealed morphological features typical of species ... | 2014 | 25204603 |
| benzo[a]pyrene modulates the biotransformation, dna damage and cortisol level of red sea bream challenged with lipopolysaccharide. | in animals, biotransformation and the immune system interact with each other, however, knowledge of the toxic mechanism of benzo[a]pyrene (bap) on these two systems is not well known. the present study investigated the toxic effects of bap on the biotransformation system, cortisol level and dna integrity of red sea bream (pagrus major). the results showed that cortisol level was induced under the challenge of lipopolysaccharide (lps). short-term exposure (96 h) of bap at environmental concentrat ... | 2014 | 24882445 |
| physicochemical responses and quality changes of red sea bream (pagrosomus major) to gum arabic coating enriched with ergothioneine treatment during refrigerated storage. | the combined effects of gum arabic coating (ga) and ergothioneine (er) treatment on the sensory and physicochemical characteristics of red sea bream (pagrosomus major) stored at 4 ± 1 °c for 16 days were investigated. fish proximate composition, ph value, total volatile basic nitrogen (tvb-n), thiobarbituric acid (tba), k-value, tca-soluble peptides, colour, texture profile analyses (tpa), microbiological properties and sensory quality were measured. the results indicate that treatment with gum ... | 2014 | 24799212 |
| comparative study of bioconcentration and erod activity induction in the japanese flounder, red sea bream, and java medaka exposed to polycyclic aromatic hydrocarbons. | japanese flounder (paralichthys olivaceus), red sea bream (pagrus major), and java medaka (oryzias javanicus) were exposed to water borne polycyclic aromatic hydrocarbons (pahs) for 10 days to compare pah bioconcentration and p450 enzyme induction by ethoxyresorufin-o-deethylase (erod) activity for use in oil spill biomonitoring in asian waters. target exposure concentration for phenanthrene, pyrene, and chrysene were 30 microg/l each, while benzo[a]pyrene was 3.0 microg/l. phenanthrene and pyre ... | 2008 | 18214891 |
| effects of dietary supplementation of ferulic acid and gamma-oryzanol on integument color and suppression of oxidative stress in cultured red sea bream, pagrus major. | the effects of ferulic acid (fa) and gamma-oryzanol (oz) supplementation on cultured red sea bream were examined. commercial brown fish meal diets supplemented with fa (0.01-0.5%) or oz (0.05-0.5%) were given to zero-year, cultured red sea bream for 98 days. after the experiment, the brightness of the integument color ("l" value) of fa- and oz-administrated fish was higher than that of control fish. furthermore, 2-thiobarbituric acid reactive substances (tbars) in the liver of fa- and oz-adminis ... | 2008 | 18198470 |
| the selenium accumulation and its effect on growth, and haematological parameters in red sea bream, pagrus major, exposed to waterborne selenium. | juvenile pagrus major (mean length 15.8±1.6cm, and mean weight 90.4±4.7g) were exposed for 4 weeks with waterborne selenium concentration (0, 50, 100, 200, and 400µg/l). the profile of se accumulation among tissue in p. major is dependent on the exposure periods and se concentration. after 4 weeks, the highest accumulation of se was observed in the kidney, and the order of se accumulation in tissues was kidney≈liver>spleen>intestine>gill>muscle. se decreased the growth rate, and there was an inv ... | 2014 | 24636952 |
| toxicity of degradation products of the antifouling biocide pyridine triphenylborane to marine organisms. | we evaluated the acute toxicities of the main degradation products of pyridine triphenylborane (ptpb), namely, diphenylborane hydroxide (dpb), phenylborane dihydroxide (mpb), phenol, and biphenyl, to the alga skeletonema costatum, the crustacean tigriopus japonicus, and two teleosts, the red sea bream pagrus major and the mummichog fundulus heteroclitus. dpb was the most toxic of the degradation products to all four organisms. the acute toxicity values of dpb for s. costatum, t. japonicus, red s ... | 2013 | 23929384 |
| status and future perspectives of vaccines for industrialised fin-fish farming. | fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ... | 2013 | 23769873 |
| molecular cloning and tissue distribution of hyaluronan binding protein 2 (habp2) in red sea bream pagrus major. | previously we have isolated a novel gelatinolytic serine proteinase, named g1, from the muscle and the plasma of red sea bream. in order to clarify the structure and function of g1, we cloned the full-length cdna of g1 from the hepatopancreas of red sea bream. g1 cdna encoded 633 amino acids with a secretory signal sequence at n-terminus, three epidermal growth factor-like domains, a kringle domain, and a trypsin-like serine protease domain. the active site residues of a serine proteinase were c ... | 2013 | 23735302 |
| effect of seasonal variation in seawater dissolved mercury concentrations on mercury accumulation in the muscle of red sea bream (pagrus major) held in minamata bay, japan. | japanese stingfish (sebastiscus marmoratus) and bambooleaf wrasse (pseudolabrus japonicas) are monitored annually for mercury pollution in minamata bay, japan. the average total mercury concentration in the muscle of these two species in minamata bay was 0.36 mg kg(-1) wet weight and 0.20 kg(-1) wet weigh, respectively, between 2008 and 2010. this is higher than levels elsewhere in japan (0.125 mg kg(-1) wet weight and 0.038 mg kg(-1) wet weight, respectively). the fda (2001) and epa (2004) sugg ... | 2013 | 23397539 |
| studies on fish scale collagen of pacific saury (cololabis saira). | we purified and characterized type i collagen from the scales of the pacific saury (cololabis saira) and compared it with collagen from other organisms. subunit composition of c. saira collagen (2α1+α2) was similar to that of red sea bream (pagrus major) and porcine collagen. c. saira collagen did not form a firm gel after neutralization of ph in solution. the temperature of denaturation (24-25 °c) of c. saira collagen was slightly lower than that of p. major collagen (26-27 °c). the contents of ... | 2013 | 25428059 |