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further characterization of the gonad-specific virus of corn earworm, helicoverpa zea.the gonad-specific virus (gsv) is a dna virus infecting the reproductive tracts of adults of both sexes of the corn earworm, helicoverpa zea, causing severe tissue deformities leading to sterility. atypical occlusion bodies containing large concentrations of virions embedded in a granular matrix were seen in the lumen of the oviduct and the bursa copulatrix of infected females. the virus, transmitted by both sexes, was successfully propagated in vivo and in tissue culture. the gsv genome is abou ...200010963397
acquisition, persistence, and species susceptibility of the hz-2v virus.hz-2v, formerly called gonad-specific virus, is known to infect the reproductive organs of both males and females of the corn earworm helicoverpa zea, rendering them agonadal or sterile. the primary mode of transmission is through mating by asymptomatic carrier moths. in this report we show that hz-2v can be acquired by first instar larvae, through feeding on virus laced diet, although the incidence of agonadal condition was significantly lower. in a laboratory study, the virus appeared to persi ...200616904685
replication of the gonad-specific virus hz-2v in ld652y cells mimics replication in vivo.a newly discovered, nonoccluded insect virus, known as gonad-specific virus or hz-2v, was found to replicate differently in two insect cell lines derived from ovarian tissues (tn-368 cells from trichoplusia ni and ld652y from lymantria dispar). differences between these two cell lines were observed in virus plaque forming ability, rate of viral dna replication, time course of infectious virus production, and the mechanism of virus release from infected cells. replication of hz-2v in ld652y cells ...200111161993
replication of a gonad-specific insect virus in tn-368 cells in culturea newly discovered, nonoccluded, insect virus, known as gonad-specific virus (gsv) was found to replicate in trichoplusia ni (tn-368) tissue culture cells. light-microscope observations indicated that 90% of the infected cells showed cytopathic effects by 2 days postinoculation. electron-microscopic observations revealed the productive replication of this nonoccluded virus with enveloped virus particles clearly visible in the nucleus of infected cells. these particles had approximately the same ...19979281395
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