| molecular characterization of a new potyvirus infecting sunflower. | we have sequenced 1873 nucleotides from the 3'-end region of a sunflower potyvirus genome including the 3'-nib protein coding region (813 nucleotides), the entire coat protein coding region (807 nucleotides) and 3'-ncr (253 nucleotides), excluding the poly (a) tail. amino acids identity of the whole cp between the sunflower virus and potyvirus members ranged from 49.5% (scmv) to 81.5% (pvy-nsnr), and the core ranged from 55% (tvmv) to 87% (pvy-nsnr; pepmov). the 3'-ncr nucleotides showed 38.7% h ... | 2000 | 11205115 |
| bidens mosaic virus is a member of the potato virus y species. | the nucleotide sequence of the genomic 3' terminal region (1,702 bases) of two brazilian bidens mosaic virus isolates (bimv: bimv-p and bimv-b) was determined. bimv-p and bimv-b share 98% nucleotide sequence identity, and are most closely related to members of the potyvirus species: sunflower chlorotic mottle virus (an isolate of potato virus y) and potato virus y. bimv-p shares 88% capsid protein amino acid identity and 77% 3'utr nucleotide sequence identity with sucmov an isolate of sunflower ... | 2006 | 16791418 |
| are sunflower chlorotic mottle virus infection symptoms modulated by early increases in leaf sugar concentration? | symptom development in a susceptible sunflower line inoculated with sunflower chlorotic mottle virus (sucmov) was followed in the second pair of leaves at different post-inoculation times: before symptom expression (bs), at early (es) and late (ls) symptom expression. sugar and starch increases and photoinhibition were observed as early effects bs, and were maintained or enhanced later on, however, chlorophyll loss was detected only at ls. photoinhibition correlated with a drastic decrease in d1 ... | 2010 | 20413182 |
| molecular characterization of sunflower chlorotic mottle virus: a member of a distinct species in the genus potyvirus. | the complete nucleotide (nt) and deduced amino acid (aa) sequences of the c (common) and crs (chlorotic ringspot) argentine strains of sucmov have been determined. the sucmov-c rna genome consists of 9,965 nt, whereas indels within the p1 coding region of sucmov-crs make its genomic length 15 nt shorter. nucleotide and aa sequence identities between the polyproteins of the c and crs strains of sucmov were 92.3 and 95.6%, respectively. pairwise comparisons between the polyproteins of the c and cr ... | 2010 | 20517623 |
| complete genome sequence of arracacha mottle virus. | arracacha mottle virus (amov) is the only potyvirus reported to infect arracacha (arracacia xanthorrhiza) in brazil. here, the complete genome sequence of an isolate of amov was determined to be 9,630 nucleotides in length, excluding the 3' poly-a tail, and encoding a polyprotein of 3,135 amino acids and a putative p3n-pipo protein. its genomic organization is typical of a member of the genus potyvirus, containing all conserved motifs. its full genome sequence shared 56.2 % nucleotide identity w ... | 2013 | 23001696 |
| development of a full-length infectious clone of sunflower chlorotic mottle virus (sucmov). | a full-length cdna clone (p35sucmov) of the sunflower chlorotic mottle virus common strain (sucmov-c) genomic rna was constructed. three cdna fragments covering the whole genome of sucmov-c were cloned between a cauliflower mosaic virus 35s promoter and a nopaline synthase terminator. mechanical inoculation of sunflower and nicotiana occidentalis seedlings with p35sucmov dna led to systemic infection. symptoms induced by p35sucmov were similar to those caused by the wild-type sucmov-c but appear ... | 2013 | 23081677 |
| complete genome sequence of sunflower ring blotch virus, a new potyvirus infecting sunflower in argentina. | the complete genome sequence of sunflower ring blotch virus (surbv), a previously undescribed potyvirus infecting sunflower in argentina, is reported. the surbv genome comprises 9555 nucleotides (nt) and encodes a polyprotein of 3061 amino acids, flanked by 5' and 3' untranslated regions of 117 and 255 nt, respectively. phylogenetic analysis showed that surbv belongs to the potato virus y (pvy) subgroup and clusters together with sunflower chlorotic mottle virus and bidens mosaic virus. percenta ... | 2017 | 28224251 |
| biological and molecular characterisation of bidens mosaic virus supports its assignment as a member of a distinct species in the genus potyvirus. | the complete nucleotide (nt) sequence of bidens mosaic virus (bimv) isolate sp01 was determined and shown to consist of 9,557 nt. since it shared highest identities in the nt sequence of the whole genome (66-73 %) and in the aa sequence of the polyprotein (60-76 %) with viruses of the potato virus y subgroup, it was compared with them genetically and biologically. phylogenetic analysis showed that the closest relative of bimv is sunflower chlorotic mottle virus, from which it, however, differed ... | 2014 | 24634052 |
| [sequence variation and protein structure of pipo gene in potato virus y]. | the objectives of this study were to understand the sequence variation and the putative protein structure of pipo gene in the potato virus y (pvy) collected from solanum tuberosum. the pipo gene in pvy was cloned using a pair of degenerate primers designed from its conserved region and its sequences were used to re-construct phylogenetic tree in potyvirus genera by a bayesian inference method. an expected fragment of 235 bp was amplified in all 20 samples by rt-pcr and the pipo genes in the 20 s ... | 2013 | 24400487 |
| sequencing of two sunflower chlorotic mottle virus isolates obtained from different natural hosts shed light on its evolutionary history. | sunflower chlorotic mottle virus (sucmov), the most prevalent virus of sunflower in argentina, was reported naturally infecting not only sunflower but also weeds. to understand sucmov evolution and improve the knowledge on its variability, the complete genomic sequences of two sucmov isolates collected from dipsacus fullonum (-dip) and ibicella lutea (-ibi) were determined from three overlapping cdna clones and subjected to phylogenetic and recombination analyses. sucmov-dip and -ibi genomes wer ... | 2013 | 22975998 |
| redox-related metabolites and gene expression modulated by sugar in sunflower leaves: similarities with sunflower chlorotic mottle virus-induced symptom. | sugars are part of an integrated redox system, since they are key regulators of respiration and photosynthesis, and therefore of the levels of reducing power, atp and ros. these elements are major determinants of the cellular redox state, which is involved in the perception and regulation of many endogenous and environmental stimuli. our previous findings suggested that early sugar increase produced during compatible sunflower chlorotic mottle virus (sucmov) infection might modulate chlorotic sy ... | 2013 | 23321504 |
| the chlorotic symptom induced by sunflower chlorotic mottle virus is associated with changes in redox-related gene expression and metabolites. | systemic infections are commonly associated with changes in host metabolism and gene expression. sunflower chlorotic mottle virus (sucmov) causes systemic infection with sugar increase, photoinhibition and increase in antioxidant enzyme activities before chlorotic symptom appearance in sunflower leaves. the aim of this study was to determine if chlorotic symptom development induced by sucmov infection is accompanied by changes in different redox-related metabolites and transcripts. symptom devel ... | 2012 | 23017905 |