Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
the genetic structure of an invasive pest, the asian citrus psyllid diaphorina citri (hemiptera: liviidae).the asian citrus psyllid diaphorina citri is currently the major threat to the citrus industry as it is the vector of candidatus liberibacter, the causal agent of huanglongbing disease (hlb). d. citri is native to asia and now colonizes the americas. although it has been known in some countries for a long time, invasion routes remain undetermined. there are no efficient control methods for the hlb despite the intensive management tools currently in use. we investigated the genetic variability an ...201425545788
first record of diaphorina citri (hemiptera: psyllidae) in ecuador infesting urban citrus and orange jasmine trees.adults and nymphs of the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae), were collected in the guayaquil, samborondón, and durán cantons in coastal ecuador. psyllids were found in high numbers in citrus (citrus spp., sapindales: rutaceae) and orange jasmine (murraya exotica [l.] jack, sapindales: rutaceae) trees within the guayaquil-samborondon-duran conurbation; however, none was found during scoutings in the main citrus producing areas in coastal ecuador. to the best of ...201425527601
insecticidal suppression of asian citrus psyllid diaphorina citri (hemiptera: liviidae) vector of huanglongbing pathogens.diaphorina citri vectors pathogens that cause 'huanglongbing' or citrus greening disease which poses a serious threat to citrus production worldwide. vector suppression is critical to reduce disease spread. efficacy is a main concern when choosing an insecticide. insecticidal treatments of 49 products or 44 active ingredients (a.i) labeled or experimental were field tested between 2005-2013 as foliar sprays (250 treatments, 39 a.i) or soil applications (47 treatments, 9 a.i) to control d. citri ...201425437858
double-stranded rna uptake through topical application, mediates silencing of five cyp4 genes and suppresses insecticide resistance in diaphorina citri.silencing of genes through rna interference (rnai) in insects has gained momentum during the past few years. rnai has been used to cause insect mortality, inhibit insect growth, increase insecticide susceptibility, and prevent the development of insecticide resistance. we investigated the efficacy of topically applied dsrna to induce rnai for five cytochrome p450 genes family 4 (cyp4) in diaphorina citri. we previously reported that these cyp4 genes are associated with the development of insecti ...201425330026
nested-quantitative pcr approach with improved sensitivity for the detection of low titer levels of candidatus liberibacter asiaticus in the asian citrus psyllid, diaphorina citri kuwayama.candidatus liberibacter asiaticus (clas) is a phloem-limited bacterium transmitted by the asian citrus psyllid, diaphorina citri, and the presumptive causal agent of citrus greening disease. the current method of detection for clas within plant and insect samples is by a presence/absence qpcr assay using the clas 16s rdna gene target. although qpcr is highly sensitive, low bacterial titers or suboptimal qpcr conditions can result in false-negatives. using a nested qpcr assay, we determined the f ...201424769405
host range testing of tamarixia radiata (hymenoptera: eulophidae) sourced from the punjab of pakistan for classical biological control of diaphorina citri (hemiptera: liviidae: euphyllurinae: diaphorinini) in california.abstract tests evaluating the host range of tamarixia radiata (waterson) (hymenoptera: eulophidae), a parasitoid of the pestiferous asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), sourced from the punjab of pakistan, were conducted in quarantine at the university of california, riverside, ca. seven nontarget psyllid species (five native and two self-introduced species) representing five families were exposed to t radiata under the following three different exposure scenari ...201424665694
characterization of the asian citrus psyllid transcriptome.the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae) is a vector for the causative agents of huanglongbing, which threatens citrus production worldwide. this study reports and discusses the first d. citri transcriptomes, encompassing the three main life stages of d. citri, egg, nymph and adult. the transcriptomes were annotated using gene ontology (go) and insecticide-related genes within each life stage were identified to aid the development of future d. citri insecticides ...201424511328
the phloem-sap feeding mealybug (ferrisia virgata) carries 'candidatus liberibacter asiaticus' populations that do not cause disease in host plants.'candidatus liberibacter asiaticus' (las) is the primary causal agent of huanglongbing (hlb), the most devastating disease of citrus worldwide. there are three known insect vectors of the hlb-associated bacteria, and all are members of the hemiptera: diaphorina citri (psyllidae), trioza erytreae (triozidae), and cacopsylla (psylla) citrisuga (psyllidae). in this study, we found that another hemipteran, the striped mealybug ferrisia virgata (cockerell) (hemiptera: pseudococcidae), was able to acq ...201424465578
innate and conditioned responses to chemosensory and visual cues in asian citrus psyllid, diaphorina citri (hemiptera: liviidae), vector of huanglongbing pathogens.asian citrus psyllid (diaphorina citri) transmits huanglongbing, a devastating disease that threatens citrus trees worldwide. a better understanding of the psyllid's host-plant selection process may lead to the development of more efficient means of monitoring it and predicting its movements. since behavioral adaptations, such as associative learning, may facilitate recognition of suitable host-plants, we examined whether adult d. citri could be conditioned to visual and chemosensory stimuli fro ...201426462949
semiochemical and vibrational cues and signals mediating mate finding and courtship in psylloidea (hemiptera): a synthesis.mate finding and courtship involve complex interactions that require close coordination between individuals of the opposite gender. well-organized signalling systems, sometimes involving a combination of signal modalities, are required to convey species-specific and individual information to members of the opposite gender. previous studies of psyllids have focused on single-signal modalities and have largely ignored the potentially interdependent nature of different types of signals. several stu ...201426462826
chemical control of the asian citrus psyllid and of huanglongbing disease in citrus.by 2014, huanglongbing (hlb), the most destructive disease of citrus, and its insect vector, the asian citrus psyllid (acp), diaphorina citri (kuwayama), became established in all major citrus-growing regions of the world, including the united states, with the exception of california. at present, application of insecticides is the most widely followed option for reducing acp populations, while application of antibiotics for suppressing hlb disease/symptoms is being practiced in some citrus-growi ...201525491482
the push-pull strategy for citrus psyllid control.the asian citrus psyllid (acp), diaphorina citri kuwayama (hemiptera: psyllidae), is the only natural vector of candidatus liberibacter asiaticus that causes citrus huanglongbing (hlb), a most destructive disease of citrus. currently, no remedial therapy exists for the disease, and so effective control of acp is very important in curbing the transmission of the disease. the push-pull strategy should be thoroughly explored as an approach to acp management. this mini-review summarises the current ...201525256398
lethal and sublethal impacts of acaricides on tamarixia radiata (hemiptera: eulophidae), an important ectoparasitoid of diaphorina citri (hemiptera: liviidae).the use of synthetic acaricides for management of pest mites may alter the efficacy of the ectoparasitoid tamarixia radiata (waterston) in biological control of diaphorina citri kuwayama, the vector of the bacteria associated with huanglongbing (hlb) in citrus orchards. we evaluated the toxicity of 16 acaricides that are recommended for the control of citrus-pest mites to t. radiata. acrinathrin, bifenthrin, carbosulfan, and fenpropathrin caused high acute toxicity and were considered harmful (m ...201526453716
changes in variable number of tandem repeats in 'candidatus liberibacter asiaticus' through insect transmission.citrus greening (huanglongbing) is the most destructive citrus disease worldwide. the disease is associated with three species of 'candidatus liberibacter' among which 'ca. liberibacter asiaticus' has the widest distribution. 'ca. l. asiaticus' is commonly transmitted by a phloem-feeding insect vector, the asian citrus psyllid diaphorina citri. a previous study showed that isolates of 'ca. l. asiaticus' were clearly differentiated by variable number of tandem repeat (vntr) profiles at four loci ...201526402645
comprehensive meta-analysis, co-expression, and mirna nested network analysis identifies gene candidates in citrus against huanglongbing disease.huanglongbing (hlb), the most devastating disease of citrus, is associated with infection by candidatus liberibacter asiaticus (calas) and is vectored by the asian citrus psyllid (acp). recently, the molecular basis of citrus-hlb interactions has been examined using transcriptome analyses, and these analyses have identified many probe sets and pathways modulated by calas infection among different citrus cultivars. however, lack of consistency among reported findings indicates that an integrative ...201526215595
whole-genome sequence of "candidatus profftella armatura" from diaphorina citri in guangdong, china.the genome of "candidatus profftella armatura" strain ycpa from diaphorina citri in guangdong, china, was sequenced. the strain has a chromosome of 457,565 bp, 24.3% g+c content, 364 predicted open reading frames (orfs), and 38 rnas, and a plasmid, pycpa54, of 5,458 bp with 23.9% g+c content and 5 orfs.201526543117
effect of cyantraniliprole, a novel insecticide, on the inoculation of candidatus liberibacter asiaticus associated with citrus huanglongbing by the asian citrus psyllid (hemiptera: liviidae).the asian citrus psyllid (diaphorina citri kuwayama) is the principal vector of 'candidatus liberibacter asiaticus' (clas) associated with huanglongbing (hlb), the most serious citrus disease worldwide. new control measures including pesticides are urgently needed to combat hlb, especially to protect young or newly planted citrus trees from clas-inoculation by vector psyllids. here, we tested clas-inoculation by d. citri adults (clas-exposed, reared on infected plants) by feeding them for 7 d on ...201526470150
diaphorina citri (hemiptera: liviidae) abundance in puerto rico declines with elevation.diaphorina citri kuwayama is the primary vector of huanglongbing, the most devastating disease of citrus. d. citri populations in puerto rico were monitored with yellow sticky traps on citrus trees or other psyllid host plants at different elevations, ranging from 10 to 880 m above sea level. trapping was conducted in march through may of 2013 and 2014 when psyllid populations usually are highest. population levels of d. citri, based on the trapping data, varied among the sites, and there was a ...201526470127
effect of uv-blocking plastic films on take-off and host plant finding ability of diaphorina citri (hemiptera: liviidae).the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), is a major pest of citrus worldwide due to its ability to transmit the bacteria associated with huanglongbing. vision, behavior, and performance of insect pests can be manipulated by using ultraviolet (uv)-blocking materials. thus, the aim of our study was to evaluate how uv-blocking plastic films may affect the take-off and host plant finding ability of d. citri. to assess the effect of a uv-deficient environment on take ...201526470126
seasonal movement patterns and long-range dispersal of asian citrus psyllid in florida citrus.the asian citrus psyllid, diaphorina citri kuwayama, is the vector of the bacterial pathogen, candidatus liberibacter asiaticus, which is the causal agent of huanglongbing (hlb) in the united states. both short-range and long-range dispersal of d. citri adults affect the spread of hlb; however, little is known about the long-range dispersal capabilities of d. citri in the field or the seasonality of flight behavior. in the present study, an in situ protein marking technique was used to determine ...201526470097
bionomics of asian citrus psyllid (hemiptera: liviidae) associated with orange jasmine hedges in southeast central florida, with special reference to biological control by tamarixia radiata.the asian citrus psyllid, diaphorina citri kuwayama, is an important pest in florida because it transmits bacteria responsible for citrus huanglongbing disease. in addition to infesting citrus, orange jasmine (murraya exotica l.) is one of asian citrus psyllid's preferred host plants and is widely grown as an ornamental hedge. we report on asian citrus psyllid bionomics over three years at five urban plantings of orange jasmine and on biological control of asian citrus psyllid by a parasitoid ta ...201526470246
host range testing of diaphorencyrtus aligarhensis (hymenoptera: encyrtidae) for use in classical biological control of diaphorina citri (hemiptera: liviidae) in california.host range tests for diaphorencyrtus aligarhensis (shafee, alam, & agarwal) (hymenoptera: encyrtidae), an endoparasitoid of asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), sourced from punjab pakistan, were conducted in quarantine at the university of california, riverside, ca. seven nontarget psyllid species representing four psyllid families were exposed to mated d. aligarhensis females in four different treatment types: 1) short sequential no-choice treatments, 2) prolo ...201526470214
diaphorina citri (hemiptera: liviidae) vector competence for the citrus greening pathogen 'candidatus liberibacter asiaticus'.characterizing the vector competence of diaphorina citri kuwayama for 'candidatus liberibacter asiaticus,' the pathogen causing citrus greening, is essential for understanding the epidemiology of this disease that is threatening the u.s. citrus industry. vector competence studies have been difficult because of the biology of d. citri, the inability to culture the pathogen, and the available diagnostic methods used to detect the bacteria in plant and insect tissues. the methods employed in many s ...201526470202
sampling methods for detection and monitoring of the asian citrus psyllid (hemiptera: psyllidae).the asian citrus psyllid (acp), diaphorina citri kuwayama is a key pest of citrus due to its role as vector of citrus greening disease or "huanglongbing." acp monitoring is considered an indispensable tool for management of vector and disease. in the present study, datasets collected between 2009 and 2013 from 245 citrus blocks were used to evaluate precision, sensitivity for detection, and efficiency of five sampling methods. the number of samples needed to reach a 0.25 standard error-mean rati ...201526313984
lethal and sublethal effects of insecticides used on citrus, on the ectoparasitoid tamarixia radiata.huanglongbing (hlb) is a disease associated with the bacteria "candidatus liberibacter spp." and has been devastating citrus orchards around the world. its management involves control of the insect vector, the asian citrus psyllid diaphorina citri kuwayama. however, the indiscriminate use of chemicals has caused pest outbreaks and eliminated the natural enemies of the vector, such as the parasitoid tamarixia radiata (waterston), the main agent for biological control of d. citri. this study asses ...201526132327
morphometric comparisons of diaphorina citri (hemiptera: liviidae) populations from iran, usa and pakistan.the asian citrus psyllid (acp), diaphorina citri kuwayama (hemiptera: liviidae), vector of citrus greening disease pathogen, huanglongbing (hlb), is considered the most serious pest of citrus in the world. prior molecular based studies have hypothesized a link between the d. citri in iran and the usa (florida). the purpose of this study was to collect morphometric data from d. citri populations from iran (mtcoi haplotype-1), florida (mtcoi haplotype-1), and pakistan (mtcoi haplotype-6), to deter ...201526038715
asymptomatic spread of huanglongbing and implications for disease control.huanglongbing (hlb) is a bacterial infection of citrus trees transmitted by the asian citrus psyllid diaphorina citri. mitigation of hlb has focused on spraying of insecticides to reduce the psyllid population and removal of trees when they first show symptoms of the disease. these interventions have been only marginally effective, because symptoms of hlb do not appear on leaves for months to years after initial infection. limited knowledge about disease spread during the asymptomatic phase is e ...201526034273
imidacloprid sorption kinetics, equilibria, and degradation in sandy soils of florida.imidacloprid (imd) is a neonicotinoid insecticide soil-drenched on sandy soils of southwest florida for the control of diaphorina citri kuwayama or asian citrus psyllid (acp). the acp vectors causal pathogens of a devastating citrus disease called citrus greening. understanding the behavior of imd in these soils and plants is critical to its performance against target pests. samples from immokalee fine sand (ifs) were used for sorption kinetics and equilibria experiments. imd kinetics data were ...201525938475
infection density dynamics of the citrus greening bacterium "candidatus liberibacter asiaticus" in field populations of the psyllid diaphorina citri and its relevance to the efficiency of pathogen transmission to citrus plants.huanglongbing, or citrus greening, is a devastating disease of citrus plants recently spreading worldwide, which is caused by an uncultivable bacterial pathogen, "candidatus liberibacter asiaticus," and vectored by a phloem-sucking insect, diaphorina citri. we investigated the infection density dynamics of "ca. liberibacter asiaticus" in field populations of d. citri with experiments using field-collected insects to address how "ca. liberibacter asiaticus" infection density in the vector insect ...201525819961
internal extracellular bacteria of diaphorina citri kuwayama (hemiptera: psyllidae), the asian citrus psyllid.the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae), is an invasive insect pest that transmits candidatus liberibacter spp. this insect/pathogen system was first identified in north america in the early 2000's and has become the top threat to the citrus industry. limited options for management of this problem exist; therefore, innovative pest management strategies are being developed. in this study, we describe the first step toward a paratransgenic approach (also referred ...201525645736
effects of methoprene, a juvenile hormone analog, on survival of various developmental stages, adult emergence, reproduction and behavior of asian citrus psyllid, diaphorina citri kuwayama.the asian citrus citrus psyllid, diaphorina citri kuwayama, transmits a bacterium that causes huanglongbing in citrus. frequent and repeated use of neurotoxic insecticides against d. citri has resulted in the development of insecticide resistance. we evaluated the effects of the juvenile hormone analog methoprene on egg hatch, nymphal development, adult emergence, reproduction and behavior of d. citri.201525583143
characterization of a recombinant cathepsin b-like cysteine peptidase from diaphorina citri kuwayama (hemiptera: liviidae): a putative target for control of citrus huanglongbing.huanglonbing (hlb) is one of the most destructive disease affecting citrus plants. the causal agent is associated with the phloem-limited bacterium candidatus liberibacter asiaticus (clas) and the psyllid diaphorina citri, vector of disease, that transmits the bacterium associated with hlb. the control of disease can be achieved by suppressing either the bacterium or the vector. among the control strategies for hlb disease, one of the widely used consists in controlling the enzymes of the diseas ...201526717484
diverse array of new viral sequences identified in worldwide populations of the asian citrus psyllid (diaphorina citri) using viral metagenomics.the asian citrus psyllid, diaphorina citri, is the natural vector of the causal agent of huanglongbing (hlb), or citrus greening disease. together; hlb and d. citri represent a major threat to world citrus production. as there is no cure for hlb, insect vector management is considered one strategy to help control the disease, and d. citri viruses might be useful. in this study, we used a metagenomic approach to analyze viral sequences associated with the global population of d. citri. by sequenc ...201526676774
living on the edges: spatial niche occupation of asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), in citrus groves.the spatial niche occupation of the asian citrus psyllid, diaphorina citri kuwayama, 1908, was evaluated to determine its field colonization and food resource exploitation strategies in citrus groves. mature grapefruit and sweet orange groves were surveyed as part of an area-wide program in 2009-2010 to determine d. citri population densities and between-tree distribution. in both cultivars, significantly more psyllids were found on perimeter trees throughout the study period suggesting a strong ...201526193111
metabolic interplay between the asian citrus psyllid and its profftella symbiont: an achilles' heel of the citrus greening insect vector.'candidatus liberibacter asiaticus' (clas), the bacterial pathogen associated with citrus greening disease, is transmitted by diaphorina citri, the asian citrus psyllid. interactions among d. citri and its microbial endosymbionts, including 'candidatus profftella armatura', are likely to impact transmission of clas. we used quantitative mass spectrometry to compare the proteomes of clas(+) and clas(-) populations of d. citri, and found that proteins involved in polyketide biosynthesis by the end ...201526580079
draft genome sequence of "candidatus liberibacter asiaticus" from diaphorina citri in guangdong, china.the draft genome sequence of "candidatus liberibacter asiaticus" strain ycpsy from an asian citrus psyllid (diaphorina citri) in guangdong, china, is reported here. the ycpsy strain has a genome size of 1,233,647 bp, 36.5% g+c content, 1,171 open reading frames (orfs), and 53 rnas.201526543132
morphological, molecular and virulence characterization of three lencanicillium species infecting asian citrus psyllids in huangyan citrus groves.citrus greening or huanglongbing (hlb) is caused by the infection of candidatus liberibacter spp. in citrus plants. since asian citrus psyllid is the primary vector of this bacterial pathogen, the spread of hlb can be mitigated by suppressing asian citrus psyllid populations in citrus groves using entomopathogens. to expand the current data on entomopathogens infecting asian citrus psyllids, we isolated and characterized three different entomopathogens. strains zjlsp07, zjla08, and zjlp09 infect ...201525593036
cloning and expressing a highly functional and substrate specific farnesoic acid o-methyltransferase from the asian citrus psyllid (diaphorina citri kuwayama).the asian citrus psyllid, diaphorina citri, transmits a phloem-limited bacterium, candidatus 'liberibacter' asiaticus that causes citrus greening disease. because juvenile hormone (jh) plays an important role in adult and nymphal development, we studied the final steps in jh biosynthesis in d. citri. a putative jh acid methyltransferase ortholog gene (jmtd) and its cognate cdna were identified by searching d. citri genome database. expression analysis shows expression in all life stages. in adul ...201525893162
infection of an insect vector with a bacterial plant pathogen increases its propensity for dispersal.the spread of vector-transmitted pathogens relies on complex interactions between host, vector and pathogen. in sessile plant pathosystems, the spread of a pathogen highly depends on the movement and mobility of the vector. however, questions remain as to whether and how pathogen-induced vector manipulations may affect the spread of a plant pathogen. here we report for the first time that infection with a bacterial plant pathogen increases the probability of vector dispersal, and that such movem ...201526083763
asian citrus psyllid expression profiles suggest candidatus liberibacter asiaticus-mediated alteration of adult nutrition and metabolism, and of nymphal development and immunity.the asian citrus psyllid (acp) diaphorina citri kuwayama (hemiptera: psyllidae) is the insect vector of the fastidious bacterium candidatus liberibacter asiaticus (clas), the causal agent of citrus greening disease, or huanglongbing (hlb). the widespread invasiveness of the psyllid vector and hlb in citrus trees worldwide has underscored the need for non-traditional approaches to manage the disease. one tenable solution is through the deployment of rna interference technology to silence protein- ...201526091106
acquisition, replication and inoculation of candidatus liberibacter asiaticus following various acquisition periods on huanglongbing-infected citrus by nymphs and adults of the asian citrus psyllid.the asian citrus psyllid, diaphorina citri (hemiptera: liviidae), is the primary vector of candidatus liberibacter asiaticus (las) implicated as causative agent of citrus huanglongbing (citrus greening), currently the most serious citrus disease worldwide. las is transmitted by d. citri in a persistent-circulative manner, but the question of replication of this bacterium in its psyllid vector has not been resolved. thus, we studied the effects of the acquisition access period (aap) by nymphs and ...201627441694
nucleotides, micro- and macro-nutrients, limonoids, flavonoids, and hydroxycinnamates composition in the phloem sap of sweet orange.currently, the global citrus production is declining due to the spread of huanglongbing (hlb). hlb, otherwise known as citrus greening, is caused by candidatus liberibacter asiaticus (clas) and is transmitted by the asian citrus psyllids (acp), diaphorina citri kuwayama. acp transmits clas bacterium while feeding on the citrus phloem sap. multiplication of clas in the phloem of citrus indicates that the sap contains all the essential nutrients needed for clas. in this study, we investigated the ...201627171979
invasive mutualisms between a plant pathogen and insect vectors in the middle east and brazil.complex multi-trophic interactions in vectorborne diseases limit our understanding and ability to predict outbreaks. arthropod-vectored pathogens are especially problematic, with the potential for novel interspecific interactions during invasions. variations and novelties in plant-arthropod-pathogen triumvirates present significant threats to global food security. we examined aspects of a phytoplasma pathogen of citrus across two continents. 'candidatus phytoplasma aurantifolia' causes witches' ...201628083099
oviposition behavior and survival of tamarixia radiata (hymenoptera: eulophidae), an ectoparasitoid of the asian citrus psyllid, diaphorina citri (hemiptera: liviidae), on hosts exposed to an entomopathogenic fungus, isaria fumosorosea (hypocreales: cordycipitaceae), under laboratory conditions.antagonistic interactions between the nymphal parasitoid, tamarixia radiata waterston (hymenoptera: eulophidae), and the arsef 3581 strain of the entomopathogenic fungus, isaria fumosorosea wize (hypocreales: cordycipitaceae), could disrupt biological control of the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). three interactions were evaluated under laboratory conditions at 25 °c: 1) parasitoid survival if parasitized hosts were exposed to arsef 3581 blastospores before ...201627435930
evolutionary conservation of candidate osmoregulation genes in plant phloem sap-feeding insects.the high osmotic pressure generated by sugars in plant phloem sap is reduced in phloem-feeding aphids by sugar transformations and facilitated water flux in the gut. the genes mediating these osmoregulatory functions have been identified and validated empirically in the pea aphid acyrthosiphon pisum: sucrase 1 (suc1), a sucrase in glycoside hydrolase family 13 (gh13), and aquaporin 1 (aqp1), a member of the drosophila integral protein (drip) family of aquaporins. here, we describe molecular anal ...201626896054
enhanced acquisition rates of 'candidatus liberibacter asiaticus' by the asian citrus psyllid (hemiptera: liviidae) in the presence of vegetative flush growth in citrus.the asian citrus psyllid preferentially feeds and exclusively reproduces on young, newly emerged flush shoots of citrus. asian citrus psyllid nymphs feed and complete their life stages on these flush shoots. recent studies conducted under greenhouse conditions have shown that the transmission rates of 'candidatus liberibacter asiaticus' (clas), the putative causal agent of huanglongbing disease of citrus, are enhanced when flush shoots are present. however, it is unclear if clas acquisition by m ...201627451998
repellent activity of botanical oils against asian citrus psyllid, diaphorina citri (hemiptera: liviidae).the asian citrus psyllid, diaphorina citri kuwayama, is the insect vector of the pathogen causing huanglongbing. we selected three botanical oils to evaluate behavioral activity against d. citri. in laboratory olfactometer assays, fir oil was repellent to d. citri females, while litsea and citronella oils elicited no response from d. citri females. in choice settling experiments, d. citri settled almost completely on control plants rather than on plants treated with fir oil at a 9.5 mg/day relea ...201627429006
host plant affects morphometric variation of diaphorina citri (hemiptera: liviidae).the asian citrus psyllid (acp), diaphorina citri kuwayama, is one of the most serious citrus pests worldwide due to its role as vector of huanglongbing or citrus greening disease. while some optimal plant species for acp oviposition and development have been identified, little is known of the influence of host plants on acp size and shape. our goal was to determine how size and shape of acp wing and body size varies when development occurs on different host plants in a controlled rearing environ ...201627833820
asian citrus psyllid rnai pathway - rnai evidence.diaphorina citri, known as the asian citrus psyllid, is an important pest of citrus because it transmits a phloem-limited bacteria strongly implicated in huanglongbing (citrus greening disease). emerging biotechnologies, such as rna interference, could provide a new sustainable and environmentally friendly strategy for the management of this pest. in this study, genome and functional analysis were performed to verify whether the rnai core genes are present in the asian psyllid genome and if the ...201627901078
morphological abnormalities and cell death in the asian citrus psyllid (diaphorina citri) midgut associated with candidatus liberibacter asiaticus.candidatus liberibacter asiaticus (clas) is a phloem-limited, gram-negative, fastidious bacterium that is associated with the development of citrus greening disease, also known as huanglongbing (hlb). clas is transmitted by the asian citrus psyllid (acp) diaphorina citri, in a circulative manner. two major barriers to transmission within the insect are the midgut and the salivary glands. we performed a thorough microscopic analysis within the insect midgut following exposure to clas-infected cit ...201627630042
antennal and abdominal transcriptomes reveal chemosensory genes in the asian citrus psyllid, diaphorina citri.the asian citrus psyllid, diaphorina citri is the principal vector of the highly destructive citrus disease called huanglongbing (hlb) or citrus greening, which is a major threat to citrus cultivation worldwide. more effective pest control strategies against this pest entail the identification of potential chemosensory proteins that could be used in the development of attractants or repellents. however, the molecular basis of olfaction in the asian citrus psyllid is not completely understood. th ...201627441376
species clarification of isaria isolates used as biocontrol agents against diaphorina citri (hemiptera: liviidae) in mexico.entomopathogenic fungi belonging to the genus isaria (hypocreales: cordycipitaceae) are promising candidates for microbial control of insect pests. currently, the mexican government is developing a biological control program based on extensive application of isaria isolates against diaphorina citri (hemiptera: liviidae), a vector of citrus huanglongbing disease. previous research identified three promising isaria isolates (che-cnrcb 303, 305, and 307; tentatively identified as isaria fumosorosea ...201626895870
annotation of the asian citrus psyllid genome reveals a reduced innate immune system.citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as huanglongbing. the citrus greening bacteria, candidatus liberibacter asiaticus (clas), is a persistent propagative pathogen transmitted by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). hemipterans characterized to date lack a number of insect immune genes, including those associated with the imd pathway targeting gram-negative bacteria. the d. citri draft ...201627965582
complete genome sequence of diaphorina citri-associated c virus, a novel putative rna virus of the asian citrus psyllid, diaphorina citri.we present here the complete nucleotide sequence and genome organization of a novel putative rna virus identified in field populations of the asian citrus psyllid, diaphorina citri, through sequencing of the transcriptome followed by reverse transcription-pcr (rt-pcr). we tentatively named this virus diaphorina citri-associated c virus (dcacv). dcacv is an unclassified positive-sense rna virus.201627445370
better together: association with 'candidatus liberibacter asiaticus' increases the reproductive fitness of its insect vector, diaphorina citri (hemiptera: liviidae).the duration of the evolutionary association between a pathogen and vector can be inferred based on the strength of their mutualistic interactions. a well-adapted pathogen is likely to confer some benefit or, at a minimum, exhibit low pathogenicity toward its host vector. coevolution of the two toward a mutually beneficial association appears to have occurred between the citrus greening disease pathogen, candidatus liberibacter asiaticus (las), and its insect vector, the asian citrus psyllid, di ...201627418697
essential oil variation from twenty two genotypes of citrus in brazil-chemometric approach and repellency against diaphorina citri kuwayama.the chemical composition of volatile oils from 22 genotypes of citrus and related genera was poorly differentiated, but chemometric techniques have clarified the relationships between the 22 genotypes, and allowed us to understand their resistance to d. citri. the most convincing similarities include the synthesis of (z)-β-ocimene and (e)-caryophyllene for all 11 genotypes of group a. genotypes of group b are not uniformly characterized by essential oil compounds. when stimulated with odor sourc ...201627338332
exposure to guava affects citrus olfactory cues and attractiveness to diaphorina citri (hemiptera: psyllidae).intercropping can reduce agricultural pest incidence, and represents an important sustainable alternative to conventional pest control methods. understanding the ecological mechanisms for intercropping could help optimize its use, particularly in tropical systems which present a large number of intercropping possibilities. citrus is threatened worldwide by greening disease (huanglongbing, hlb) vectored by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae). control of hlb ...201627247354
disruption of vector host preference with plant volatiles may reduce spread of insect-transmitted plant pathogens.plant pathogens can manipulate the odor of their host; the odor of an infected plant is often attractive to the plant pathogen vector. it has been suggested that this odor-mediated manipulation attracts vectors and may contribute to spread of disease; however, this requires further broad demonstration among vector-pathogen systems. in addition, disruption of this indirect chemical communication between the pathogen and the vector has not been attempted. we present a model that demonstrates how a ...201627193763
digital gene expression analysis of ponkan mandarin (citrus reticulata blanco) in response to asia citrus psyllid-vectored huanglongbing infection.citrus huanglongbing (hlb), the most destructive citrus disease, can be transmitted by psyllids and diseased budwoods. although the final symptoms of the two main hlb transmission ways were similar and hard to distinguish, the host responses might be different. in this study, the global gene changes in leaves of ponkan (citrus reticulata) mandarin trees following psyllid-transmission of hlb were analyzed at the early symptomatic stage (13 weeks post inoculation, wpi) and late symptomatic stage ( ...201627384559
imidacloprid extraction from citrus leaves and analysis by liquid chromatography-mass spectrometry (hplc-ms/ms).a procedure was developed to extract imidacloprid (imd) from newly-flushed and fully-expanded citrus leaves. the extraction was conducted in a bullet blender, using a small sample mass (0.5 g of fresh tissue), stainless-steel beads (24 g), and methanol as extractant (10 ml). the extracts did not require further clean-up before analysis by hplc-ms/ms. the method was validated with control samples from imd-untreated hamlin orange trees. the method limit of detection and limit of quantitation were ...201626988226
monitoring for insecticide resistance in asian citrus psyllid (hemiptera: psyllidae) populations in florida.the development of insecticide resistance in asian citrus psyllid, diaphorina citri kuwayama, populations is a serious threat to the citrus industry. as a contribution to a resistance management strategy, we developed a glass vial technique to monitor field populations of asian citrus psyllid for insecticide resistance. diagnostic concentrations needed to separate susceptible genotypes from resistant individuals were determined for cypermethrin (0.5 μg per vial), malathion (1.0 μg per vial), dia ...201626709293
transmission rates of ‘ca. liberibacter asiaticus’ by asian citrus psyllid are enhanced by the presence and developmental stage of citrus flush.asian citrus psyllid (diaphorina citri kuwayama) transmits a bacterium ‘candidatus liberibacter asiaticus’ (clas) putatively responsible for a devastating citrus disease known as asiatic huanglongbing (hlb) (citrus greening disease). the psyllid and disease have invaded many citrus-growing regions including the united states, where the disease is seriously jeopardizing the florida citrus industry. we recently concluded research that showed clas transmission rates are increased when citrus flush ...201626884596
thiamethoxam and imidacloprid drench applications on sweet orange nursery trees disrupt the feeding and settling behaviour of diaphorina citri (hemiptera: liviidae).chemical control is the method most used for management of diaphorina citri, the vector of the phloem-limited bacteria associated with citrus huanglongbing (hlb) disease. the objectives of this study were to determine the influence of soil-drench applications of neonicotinoids (thiamethoxam and imidacloprid) on the probing behaviour of d. citri on citrus nursery trees, using the electrical penetration graph (epg) technique, and to measure the d. citri settling behaviour after probing on citrus n ...201626694803
phenology of asian citrus psyllid (hemiptera: liviidae), with special reference to biological control by tamarixia radiata, in the residential landscape of southern california.since its discovery in 2008, the pestiferous asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), has become widely established in residential citrus trees throughout southern california. in 2011, tamarixia radiata (waterston) (hymenoptera: eulophidae), a host-specific parasitoid of d. citri, sourced from punjab pakistan, was introduced into california as part of a classical biological program aimed at suppressing d. citri populations in urban areas. despite these release effor ...201626957552
impact of different temperatures on survival and energy metabolism in the asian citrus psyllid, diaphorina citri kuwayama.temperature influences the life history and metabolic parameters of insects. asian citrus psyllid (acp), diaphorina citri is a tropical and subtropical pest. acp invaded new regions around the world and threatened the citrus industry as a vector for huanglongbing (hlb) disease. acp is widely distributed and can survive high (up to 45 °c) and low temperatures (as low as -6 °c). the precise mechanism of temperature tolerance in acp is poorly understood. we investigated adult survival, cellular ene ...201626603556
huanglongbing: an overview of a complex pathosystem ravaging the world's citrus.citrus huanglongbing (hlb) has become a major disease and limiting factor of production in citrus areas that have become infected. the destruction to the affected citrus industries has resulted in a tremendous increase to support research that in return has resulted in significant information on both applied and basic knowledge concerning this important disease to the global citrus industry. recent research indicates the relationship between citrus and the causal agent of hlb is shaped by multip ...201626466921
formic and acetic acids in degradation products of plant volatiles elicit olfactory and behavioral responses from an insect vector.volatile phytochemicals play a role in orientation by phytophagous insects. we studied antennal and behavioral responses of the asian citrus psyllid, diaphorina citri, vector of the citrus greening disease pathogen. little or no response to citrus leaf volatiles was detected by electroantennography. glass cartridges prepared with β-ocimene or citral produced no response initially but became stimulatory after several days. both compounds degraded completely in air to a number of smaller molecules ...201626857741
inter-population variability of endosymbiont densities in the asian citrus psyllid (diaphorina citri kuwayama).the asian citrus psyllid (diaphorina citri kuwayama) is an insect pest capable of transmitting candidatus liberibacter asiaticus (clas), the causal agent of citrus greening in north america. d. citri also harbors three endosymbionts, wolbachia, candidatus carsonella ruddii, and candidatus profftella armatura, which may influence d. citri physiology and fitness. although genomic researches on these bacteria have been conducted, much remains unclear regarding their ecology and inter-population var ...201626846216
transmission and propagation of 'candidatus liberibacter asiaticus' by grafting with individual citrus leaves.huanglongbing (hlb) is a chronic, progressive decline disease in citrus associated with a systemic infection by the bacterium 'candidatus liberibacter asiaticus'. transmission of the bacterium in the field is by the asian citrus psyllid, diaphorina citri kuwayama. experimental propagation of 'ca. l. asiaticus' is done primarily by grafting pieces of bud wood from an infected plant. to produce a small-scale model system for investigation of pathogen biology, we investigated grafting single leaves ...201626807818
disrupting mating behavior of diaphorina citri (liviidae).severe economic damage from citrus greening disease, caused by 'candidatus liberibacter asiaticus' bacteria, has stimulated development of methods to reduce mating and reproduction in populations of its insect vector, diaphorina citri (hemiptera: liviidae). male d. citri find mating partners by walking on host plants, intermittently producing vibrational calls that stimulate duetting replies by receptive females. the replies provide orientational feedback, assisting the search process. to test a ...201627707945
effects of candidatus liberibacter asiaticus on the fitness of the vector diaphorina citri.the asian citrus psyllid (acp), diaphorina citri kuwayama transmits the bacterium 'candidatus liberibacter asiaticus' (las), which causes citrus huanglongbing (hlb) disease. although many studies have been conducted on the biology of acp on different host plants, few have taken the plant, las bacteria and the vector insect within one context to evaluate the effects of las on the fitness of acp under field conditions. understanding the relationship between las and acp is critical for both acp and ...201627667799
two-spotted ladybeetle adalia bipunctata l. (coleoptera: coccinellidae): a commercially available predator to control asian citrus psyllid diaphorina citri (hemiptera: liviidae).the asian citrus psyllid diaphorina citri kuwayama (hemiptera: liviidae) is an economically important pest of citrus because it serves as a vector of the causal pathogens of huanglongbing (hlb) also known as citrus greening disease. the increased use of insecticides for control of d. citri negatively impacts several natural enemies including some effective ladybeetle species which are not available commercially. the two-spotted ladybeetle, adalia bipunctata (coleoptera: coccinellidae) is found i ...201627631730
complete genome sequence of the largest known flavi-like virus, diaphorina citri flavi-like virus, a novel virus of the asian citrus psyllid, diaphorina citri.a novel flavi-like virus tentatively named diaphorina citri flavi-like virus (dcflv) was identified in field populations of diaphorina citri through small rna and transcriptome sequencing followed by reverse transcription (rt)-pcr. we report here the complete nucleotide sequence and genome organization of dcflv, the largest flavi-like virus identified to date.201627609921
a review of techniques for detecting huanglongbing (greening) in citrus.huanglongbing (hlb) is the most destructive disease of citrus worldwide. monitoring of health and detection of diseases in trees is critical for sustainable agriculture. hlb symptoms are virtually the same wherever the disease occurs. the disease is caused by candidatus liberibacter spp., vectored by the psyllids diaphorina citri kuwayama and trioza erytreae. electron microscopy was the first technique used for hlb detection. nowadays, scientists are working on the development of new techniques ...201627590666
a phagostimulant blend for the asian citrus psyllid.chemical cues that elicit orientation by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), are of interest because it is the primary vector of the causal pathogen of citrus greening disease. non-pesticidal control methods for d. citri remain a high priority for the citrus industry. while searching for semiochemicals that may be involved in orientation to host plants, we previously identified a blend of formic and acetic acids that stimulated substrate probing by d. citri ...201627542089
complete genome sequence of a putative densovirus of the asian citrus psyllid, diaphorina citri.here, we report the complete genome sequence of a putative densovirus of the asian citrus psyllid, diaphorina citri diaphorina citri densovirus (dcdnv) was originally identified through metagenomics, and here, we obtained the complete nucleotide sequence using pcr-based approaches. phylogenetic analysis places dcdnv between viruses of the ambidensovirus and iteradensovirus genera.201627469948
the influence of learning on host plant preference in a significant phytopathogen vector, diaphorina citri.although specialist herbivorous insects are guided by innate responses to host plant cues, host plant preference may be influenced by experience and is not dictated by instinct alone. the effect of learning on host plant preference was examined in the asian citrus psyllid, diaphorina citri; vector of the causal agent of citrus greening disease or huanglongbing. we investigated: a) whether development on specific host plant species influenced host plant preference in mature d. citri; and b) the e ...201626930355
laser system for identification, tracking, and control of flying insects.flying insects are common vectors for transmission of pathogens and inflict significant harm to humans and agricultural production in many parts of the world. we present proof of principle for an optical system capable of highly specific vector control. this system utilizes a combination of optical sources, detectors, and sophisticated software to search, detect, and identify flying insects in real-time, with the capability of eradication using a lethal laser pulse. we present data on two insect ...201627410106
metabolomic comparative analysis of the phloem sap of curry leaf tree (bergera koenegii), orange jasmine (murraya paniculata), and valencia sweet orange (citrus sinensis) supports their differential responses to huanglongbing.orange jasmine, murraya paniculata and curry leaf tree, bergera koenegii are alternative hosts for diaphorina citri, the vector of candidatus liberibacter asiaticus (clas), the pathogen of huanglongbing (hlb) in citrus. d. citri feeds on the phloem sap where clas grows. it has been shown that orange jasmine was a better host than curry leaf tree to d. citri. in addition, clas can infect orange jasmine but not curry leaf tree. here, we compared the phloem sap composition of these 2 plants to the ...201627763819
quality matters: influences of citrus flush physicochemical characteristics on population dynamics of the asian citrus psyllid (hemiptera: liviidae).studies were conducted to relate the influence of the physical characteristics, leaf nutrient content and phloem sap amino acid concentration of citrus flush shoots on the densities of various diaphorina citri life stages. adult d. citri preferentially selected young shoots for feeding and numbers of d. citri immatures were positively correlated with flush shoot softness. young flush shoots had higher concentrations of macro and micro nutrients relative to mature ones and this was associated wit ...201628030637
diaphorina citri induces huanglongbing-infected citrus plant volatiles to repel and reduce the performance of propylaea japonica.transmission of plant pathogens through insect vectors is a complex biological process involving interactions between the host plants, insects, and pathogens. simultaneous impact of the insect damage and pathogenic bacteria in infected host plants induce volatiles that modify not only the behavior of its insect vector but also of their natural enemies, such as parasitoid wasps. therefore, it is essential to understand how insects such as the predator ladybird beetle responds to volatiles emitted ...201628083006
efficacy of essential oil of piper aduncum against nymphs and adults of diaphorina citri.insecticide application is the main way to control diaphorina citri. however, it causes environmental contamination, has a negative impact on beneficial organisms and leads to psyllid resistance. the essential oil of piper aduncum has low toxicity towards the environment and contains dillapiol, which has proven to be effective against several crop pests. here, we studied its efficacy against nymphs and adults of d. citri under laboratory conditions. oils with three concentrations of dillapiol (6 ...201626331551
effects of different temperature regimes on survival of diaphorina citri and its endosymbiotic bacterial communities.the asian citrus psyllid, diaphorina citri, is a major pest of citrus and vector of citrus greening (huanglongbing) in asian. in our field-collected psyllid samples, we discovered that fuzhou (china) and faisalabad (pakistan), populations harbored an obligate primary endosymbiont candidatus carsonella (gen. nov.) with a single species, candidatus carsonella ruddii (sp. nov.) and a secondary endosymbiont, wolbachia surface proteins (wsp) which are intracellular endosymbionts residing in the bacte ...201728618183
analyses of mitogenome sequences revealed that asian citrus psyllids (diaphorina citri) from california were related to those from florida.asian citrus psyllid (acp, diaphorina citri kuwayama) transmits "candidatus liberibacter asiaticus" (clas), an unculturable alpha-proteobacterium associated with citrus huanglongbing (hlb). clas has recently been found in california. understanding acp population diversity is necessary for hlb regulatory practices aimed at reducing clas spread. in this study, two circular acp mitogenome sequences from california (mt-capsy, ~15,027 bp) and florida (mt-flpsy, ~15,012 bp), usa, were acquired. each m ...201728860662
an assessment of interspecific competition between two introduced parasitoids of diaphorina citri (hemiptera: liviidae) on caged citrus plants.two parasitoids attacking nymphs of asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), tamarixia radiata (waterston) (hymenoptera: eulophidae) and diaphorencyrtus aligarhensis (shafee, alam & agarwal) (hymenoptera: encyrtidae) are being released in california, usa in a classical biological control program. to evaluate the effect of multiple parasitoid species on d. citri mortality, we conducted mesocosm experiments under controlled conditions using a complete block design wit ...201728590080
combining 'omics and microscopy to visualize interactions between the asian citrus psyllid vector and the huanglongbing pathogen candidatus liberibacter asiaticus in the insect gut.huanglongbing, or citrus greening disease, is an economically devastating bacterial disease of citrus. it is associated with infection by the gram-negative bacterium candidatus liberibacter asiaticus (clas). clas is transmitted by diaphorina citri, the asian citrus psyllid (acp). for insect transmission to occur, clas must be ingested during feeding on infected phloem sap and cross the gut barrier to gain entry into the insect vector. to investigate the effects of clas exposure at the gut-pathog ...201728632769
a small wolbachia protein directly represses phage lytic cycle genes in "candidatus liberibacter asiaticus" within psyllids.huanglongbing (hlb) is a severe disease of citrus caused by an uncultured alphaproteobacterium "candidatus liberibacter asiaticus" and transmitted by asian citrus psyllids (diaphorina citri). two prophage genomes, sc1 and sc2, integrated in "ca. liberibacter asiaticus" strain uf506 were described previously, and very similar prophages are found resident in the majority of "ca. liberibacter asiaticus" strains described worldwide. the sc1 lytic cycle is marked by upregulation of prophage late gene ...201728608866
ldtr is a master regulator of gene expression in liberibacter asiaticus.huanglongbing or citrus greening disease is causing devastation to the citrus industry. liberibacter asiaticus, an obligate intracellular pathogen of citrus, is one the causative agents of the disease. most of the knowledge about this bacterium has been deduced from the in silico exploration of its genomic sequence. l. asiaticus differentially expresses genes during its transmission from the psyllid vector, diaphorina citri, to the plant. however, the regulatory mechanisms for the adaptation of ...201728503858
draft whole-genome sequence of "candidatus liberibacter asiaticus" strain tx2351 isolated from asian citrus psyllids in texas, usa.we report here the draft genome sequence of "candidatus liberibacter asiaticus" strain tx2351, collected from asian citrus psyllids in south texas, usa. the tx2351 genome has a size of 1,252,043 bp, a g+c content of 36.5%, 1,184 predicted open reading frames, and 52 rna genes.201728408682
compatibility and efficacy of isaria fumosorosea with horticultural oils for mitigation of the asian citrus psyllid, diaphorina citri (hemiptera: liviidae).horticultural oils are an important component of integrated management programs of several phytophagous arthropods and pathogens affecting fruit, ornamentals and vegetables in greenhouse and field production systems. although effective against the target pest, their incompatibility with biological control agents can compromise efforts to develop eco-friendly management programs for important agricultural pests. in this study, we assessed the in vitro effect of selected refined petroleum oils use ...201729088086
rna interference of two glutathione s-transferase genes, diaphorina citri dcgste2 and dcgstd1, increases the susceptibility of asian citrus psyllid (hemiptera: liviidae) to the pesticides fenpropathrin and thiamethoxam.the asian citrus psyllid, diaphorina citri kuwayama, is an important agricultural pest of citrus globally. foliar application of chemical insecticides is the most widely used option for reducing d. citri populations. knockdown of glutathione s-transferase (gst) in several insect species leads to increased susceptibility to insecticides; however, information about the detoxifying role of gst genes in d. citri is unavailable.201728971568
induced preference improves offspring fitness in a phytopathogen vector.in the asian citrus psyllid (diaphorina citri kuwayama), learning facilitates host recognition and mate preference; however, it is unclear whether induced female oviposition preference occurs in this species. we investigated the influence of natal host experience on adult oviposition preference when reared on either 'valencia' orange (citrus x sinesis) or orange jasmine (murraya paniculata). psyllids reared on 'valencia' orange preferred 'valencia' orange as an oviposition host compared with ora ...201728961753
neonicotinoid-induced mortality of diaphorina citri (hemiptera: liviidae) is affected by route of exposure.the use of neonicotinoids in citrus (rutaceae) has increased substantially to help manage the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), a vector of the devastating citrus disease, huanglongbing (hlb). in citrus pest management programs, neonicotinoids are most often applied to the soil as a drench and move through xylem channels from the roots into the foliage. we developed a novel assay to quantify the dose required to kill d. citri following ingestion and compare i ...201728961723
concomitant loss of the glyoxalase system and glycolysis makes the uncultured pathogen "candidatus liberibacter asiaticus" an energy scavenger.methylglyoxal (mg) is a cytotoxic, non-enzymatic byproduct of glycolysis that readily glycates proteins and dna, resulting in carbonyl stress. glyoxalase i and ii (gloa and glob) sequentially convert mg into d-lactic acid using glutathione (gsh) as a cofactor. the glyoxalase system is essential for the mitigation of mg-induced carbonyl stress, preventing subsequent cell death, and recycling gsh for maintenance of cellular redox poise. all pathogenic liberibacters identified to date are unculture ...201728939611
resistance management for asian citrus psyllid, diaphorina citri kuwayama, in florida.the asian citrus psyllid, diaphorina citri kuwayma, is one of the most important pests in citrus production. the objective of this study was to evaluate d. citri resistance management with three insecticide rotations and compare them with no rotation and an untreated check. the different insecticides (modes of action) tested were: dimethoate, imidacloprid, diflubenzuron, abamectin 3% + thiamethoxam 13.9%, and fenpropathrin. eggs, nymph, and adult psyllids were counted weekly. five insecticide ap ...201728930170
imidacloprid transport and sorption nonequilibrium in single and multilayered columns of immokalee fine sand.imidacloprid (imd) is a neonicotinoid pesticide soil-drenched to many crops to control piercing-sucking insects such as the asian citrus psyllid (acp). neonicotinoids are persistent in the environment and transport analyses are helpful estimate leaching potential from soils that could result in groundwater pollution. the objective of this study was to analyze imd breakthrough under saturated water flow in soil columns packed with three horizons (a, e, bh) of immokalee fine sand (ifs). also, we u ...201728837702
wavelength and polarization affect phototaxis of the asian citrus psyllid.the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), is a primary pest of citrus due to its status as a vector of the citrus disease, huanglongbing. we evaluated the effects of light of specific wavelength and polarization on phototactic behavior of d. citri using a horizontal bioassay arena. wavelength-associated positive phototaxis was associated with short wavelength uv (350-405 nm) targets whereas little or no responses were seen in longer wavelength targets in the visi ...201728825636
'candidatus liberibacter asiaticus' accumulates inside endoplasmic reticulum associated vacuoles in the gut cells of diaphorina citri.citrus greening disease known also as huanglongbing (hlb) caused by the phloem-limited bacterium 'candidatus liberibacter asiaticus' (clas) has resulted in tremendous losses and the death of millions of trees worldwide. clas is transmitted by the asian citrus psyllid diaphorina citri. the closely-related bacteria 'candidatus liberibacter solanacearum' (clso), associated with vegetative disorders in carrots, is transmitted by the carrot psyllid bactericera trigonica. a promising approach to preve ...201729208900
genetic variation and potential co-infection of wolbachia among widespread asian citrus psyllid (diaphorina citri kuwayama) populations.wolbachia can profoundly influence the survival, reproduction, and defenses of insect hosts. these interactions could potentially be harnessed for managing pests or insect-transmitted diseases. diaphorina citri kuwayama is a phloem-feeding pest capable of transmitting the putative causal agent of citrus greening, candidatus liberibacter asiaticus (clas). like many insects, d. citri is also infected with wolbachia (wdi). recent studies indicate that the relative abundance of wdi could be associat ...201729286204
behavior of bacteriome symbionts during transovarial transmission and development of the asian citrus psyllid.the asian citrus psyllid diaphorina citri kuwayama (hemiptera: liviidae) is a serious pest worldwide, transmitting candidatus liberibacter spp. (alphaproteobacteria), the causative agents of a devastating citrus disease known as huanglongbing or greening disease. in a symbiotic organ called the bacteriome, d. citri possesses an organelle-like defensive symbiont, candidatus profftella armatura (betaproteobacteria), and a nutritional symbiont, ca. carsonella ruddii (gammaproteobacteria). drastical ...201729240843
Displaying items 101 - 200 of 242