Publications

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complete nucleotide sequence of pelargonium zonate spot virus and its relationship with the family bromoviridae.the complete sequence of the pelargonium zonate spot virus (pzsv) genome was determined. it comprises 8477 nt, distributed in three positive-strand rna species encoding four proteins. rna-1 is 3383 nt long, with an orf that encodes a polypeptide with a molecular mass of 108 419 da (denoted protein 1a). this protein contains the conserved sequence motifs i-iii of type i methyltransferases and the seven consensus motifs of the helicases of superfamily 1. rna-2 is 2435 nt long and encodes a major p ...200314573820
evolutionary relationships among members of the bromoviridae deduced from whole proteome analysis.many molecular phylogenies of viruses build upon the analysis of single genes. the study of whole-genomes, however, might yield more reliable information to infer tree topologies and a better approach for drawing the evolutionary history of virus families. in this study, we apply a novel comparative proteomic approach to seek whether incorporating information from the entire proteome would support the actual taxonomy of the family bromoviridae. our results suggest that the current taxonomic clas ...200616172839
production of polyclonal antibodies against pelargonium zonate spot virus coat protein expressed in escherichia coli and application for immunodiagnosis.pelargonium zonate spot virus (pzsv) is identified recently in tomato plants in the united states. to develop serological diagnostic tools for the detection of this virus, the production of good quality antibodies is a necessity. the coat protein (cp) gene of a california isolate of pzsv was cloned into a bacterial expression vector (ptriex-4 ek/lic). the plasmid ptriex-4-pzsv-cp was transformed into escherichia coli rosetta 2(de3)placi and the recombinant pzsv-cp was expressed as a fusion prote ...200919397928
identification of plant viruses using one-dimensional gel electrophoresis and peptide mass fingerprints.a generic assay to detect and partially characterize unknown viruses from plants was developed. proteins extracted from virus-infected and uninfected plants were separated in one dimension by sds polyacrylamide gel electrophoresis. differentially expressed protein bands were eluted after trypsin digestion and resulting peptide fragments separated according to their mass by matrix-assisted laser-desorption ionization time-of-flight (maldi-tof) mass spectrometry. resulting peptide mass fingerprint ...201020170682
pelargonium zonate spot virus is transmitted vertically via seed and pollen in tomato.in autumn 2007, a new disease with unknown etiology was observed in open-field tomato (solanum lycopersicum) in the lachish region of israel. the symptoms included mild mosaic, leaf malformation, and severe stunting of the plants. the causal agent was readily transmitted mechanically from the sap of infected plants to indicator plants. viral particles were purified from infected plants and cdna was synthesized from rna isolated from the particles. cloning and sequencing of the cdna showed 95% id ...201020626283
highly specific detection of five exotic quarantine plant viruses using rt-pcr.to detect five plant viruses (beet black scorch virus, beet necrotic yellow vein virus, eggplant mottled dwarf virus, pelargonium zonate spot virus, and rice yellow mottle virus) for quarantine purposes, we designed 15 rt-pcr primer sets. primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. all but one primer set successfully amplified the targets, and gradient pcrs indicated that the optimal temperature for the 14 useful primer s ...201325288934
cassava ivorian bacilliform virus is a member of the genus anulavirus.the complete genomic sequence of cassava ivorian bacilliform virus (cibv) is described. the virus has a genomic organization similar to that of pelargonium zonate spot virus (pzsv), the type member of the genus anulavirus, but it is most closely related to a second, recently described, anulavirus, amazon lily mild mottle virus (alimmv).201424838850
characterization of a new anulavirus isolated from amazon lily plants.a quasi-spherical virus was isolated from a cultivated amazon lily plant (eucharis grandiflora) that could be mechanically transmitted to healthy e. grandiflora plants, subsequently producing mild mosaic or mottle symptoms on the leaves. the purified virus consisted of three quasi-spherical particles about 20 nm wide and 70, 40 and 30 nm in length, containing three segmented genomes of 3,169, 2,507 and 2,530 nucleotides, respectively. sequence analysis showed that the newly isolated virus is rel ...201322965580
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