pathogenicity of isaria fumosorosea to bemisia tabaci, with some observations on the fungal infection process and host immune response.isaria fumosorosea is an important pathogen of whiteflies, and is used as a mycoinsecticide against this pest in many regions of the world. we quantified the pathogenicity of the chinese isolate if-1106 against different life stages of sweetpotato whitefly, bemisia tabaci, on cucumber plants, and describe the infection process and aspects of the host immunological response in the laboratory. the second instar was the most susceptible life stage to infection, with mortality rates at 10(7)conidia/ ...201526264671
data in support of fumosorinone, a novel ptp1b inhibitor, activates insulin signaling in insulin-resistance hepg2 cells and shows anti-diabetic effect in diabetic kkay mice.this data article contains data related to the research article entitled "fumosorinone, a novel ptp1b inhibitor, activates insulin signaling in insulin-resistance hepg2 cells and shows anti-diabetic effect in diabetic kkay mice" in the toxicology and applied pharmacology [1]. fumosorinone (fu) is a new inhibitor of protein phosphatase 1b inhibitor, which was isolated from insect pathogenic fungi isaria fumosorosea. fu was found to inhibit ptp1b activity in our previous study [2]. ptp1b is the ph ...201526217781
expression of dsrna in recombinant isaria fumosorosea strain targets the tlr7 gene in bemisia tabaci.rna interference (rnai) technology shows a great potential in controlling agricultural pests, despite the difficulty of introducing exogenous dsrna/sirna into target pests. isaria fumosorosea is a common fungal pathogen of the b-biotype bemisia tabaci (whitefly), which is a widespread pest. entomopathogenic fungi directly penetrate the cuticle and invade insect hemocoel. application of i. fumosorosea expressing dsrna of whitefly immunity-related gene may aid in developing rnai technology to effe ...201526198409
critical values of porosity in rice cultures of isaria fumosorosea by adding water hyacinth: effect on conidial yields and quality.conidia of the entomopathogenic fungus isaria fumosorosea are used to control insect pests in crops. commercially available mycoinsecticides manufactured with this fungus are produced on a large scale via solid-state cultures (ssc). in order to favour gaseous exchange in scc, texturizers can be added to increase porosity fraction (ε). this work presents results of water hyacinth (eichhornia crassipes) as a novel texturizer. a mixture of parboiled rice (pr), with a ε = 0.23, was used as a substra ...201526184013
potent anti-calmodulin activity of cyclotetradepsipeptides isolated from isaria fumosorosea using a newly designed cyclotetradepsipeptides, namely beauverolides c (1), f (2), i (3), ja (4), l (5), m (6), and n (7), were isolated from the entomopathogenic fungus isaria fumosorosea. the beauverolides were evaluated as potential calmodulin (cam) inhibitors using the newly designed cam biosensor hcam m124c-af350; these peptides displayed high affinity to the protein with dissociation constants (kd) ranging from 0.078 μm to 3.44 μm. beauverolide ja, the only one containing a tryptophan residue in its struct ...201525920232
structure and biosynthesis of fumosorinone, a new protein tyrosine phosphatase 1b inhibitor firstly isolated from the entomogenous fungus isaria fumosorosea.fumosorinone, isolated from the entomogenous fungus isaria fumosorosea, is a new 2-pyridone alkaloid which is elucidated by hresims 1d and 2dnmr. fumosorinone is structurally similar to tenellin and desmethylbassianin but it differs in chain length and degree of methylation. it is characterized by a classic noncompetitive inhibitor of protein tyrosine phosphatase 1b (ic50 14.04μm) which was implicated as a negative regulator of insulin receptor signaling and a potential drug target for the treat ...201525857260
fumosorinone, a novel ptp1b inhibitor, activates insulin signaling in insulin-resistance hepg2 cells and shows anti-diabetic effect in diabetic kkay mice.insulin resistance is a characteristic feature of type 2 diabetes mellitus (t2dm) and is characterized by defects in insulin signaling. protein tyrosine phosphatase 1b (ptp1b) is a key negative regulator of the insulin signaling pathways, and its increased activity and expression are implicated in the pathogenesis of insulin resistance. therefore, the inhibition of ptp1b is anticipated to become a potential therapeutic strategy to treat t2dm. fumosorinone (fu), a new natural product isolated fro ...201525796170
harvesting of chlorella sorokiniana by co-culture with the filamentous fungus isaria fumosorosea: a potential sustainable feedstock for hydrothermal gasification.despite recent advances in down-stream processing, production of microalgae remains substantially limited because of economical reasons. harvesting and dewatering are the most energy-intensive processing steps in their production and contribute 20-30% of total operational cost. bio-flocculation of microalgae by co-cultivation with filamentous fungi relies on the development of large structures that facilitate cost effective harvesting. a yet unknown filamentous fungus was isolated as a contamina ...201525795450
efficacy of entomopathogenic fungi (ascomycetes: hypocreales) against adult haematobia irritans (diptera: muscidae) under stable conditions in the mexican dry tropics.the purpose of this paper is to evaluate the effect of five strains of metarhizium anisopliae (ma) and three strains of isaria fumosorosea (ifr) at a concentration of 1×10(8)colony-forming units/ml applied by spraying onto bovines with controlled infestation of haematobia irritans under stable conditions in the mexican dry tropics. four experiments were performed, in each of which three treatments (two fungal strains and one control) were evaluated with eight repetitions for each one, by carryin ...201525771932
optimization and synthesis of silver nanoparticles using isaria fumosorosea against human vector mosquitoes.the efficacy of silver generated larvicide with the help of entomopathogenic fungi, isaria fumosorosea (ifr) against major vector mosquitoes culex quinquefasciatus and aedes aegypti. the ifr-silver nanoparticles (agnps) were characterized structurally and functionally using uv-visible spectrophotometer followed by scanning electron microscopy (sem), energy-dispersive x-ray (edx) spectroscopy and fourier transform infrared (ftir) spectra. the optimum ph (alkaline), temperature (30 °c) and agitati ...201425085201
enhancing the thermotolerance of entomopathogenic isaria fumosorosea sfp-198 conidial powder by controlling the moisture content using drying and adjuvants.entomopathogenic fungi are promising pest-control agents but their industrial applicability is limited by their thermosusceptibility. with an aim to increase the thermotolerance of isaria fumosorosea sfp-198, moisture absorbents were added to dried conidial powder, and the relationship between its water potential and thermotolerance was investigated. mycotized rice grains were dried at 10℃, 20℃, 30℃, and 40℃ and the drying effect of each temperature for 24, 48, 96, and 140 hr was determined. dry ...201424808736
extracellular lipase of an entomopathogenic fungus effecting larvae of a scale insect.lipases play an important role in the infection process of entomopathogenic fungi by hydrolyzing the ester bonds of lipoproteins, fats and waxes present on the insect surface and in the body. here we report the purification and characterization of an extracellular lipase from isaria fumosorosea. the enzyme was purified (138.46-fold) in three steps using (nh4 )2 so4 precipitation followed by deae-cellulose and sephadex g-100 column chromatography. the molecular weight of purified enzyme was deter ...201424677050
enhanced fungicide resistance in isaria fumosorosea following ionizing radiation-induced mutagenesis.the application of entomopathogenic fungi such as isaria fumosorosea to combat insect pests on plants is complicated by their sensitivity to commonly used fungicides. in this study, i. fumosorosea mutants with enhanced resistance to the fungicide benomyl were induced by irradiation using either ion beams or gamma rays, or a combination of the two. when grown on agar containing benomyl, mycelial growth was observed for five of the six mutant isolates at benomyl concentrations that were more than ...201324164561
oxygen-rich culture conditions enhance the conidial infectivity and the quality of two strains of isaria fumosorosea for potentially improved biocontrol addition to high production levels of conidia, the success of entomopathogenic fungi as biological control agents depends both on their prevalence under the environmental conditions found in open fields (resistance to stress) and on the capacity of these conidia to infect pests. this study compares conidium production, infectivity and resistance to thermal and osmotic stress in two strains of isaria fumosorosea (arsef 3302 and cnrcb1) grown either under a normal atmosphere (21% o2) or using e ...201423847155
antioxidant enzyme influences germination, stress tolerance, and virulence of isaria fumosorosea.antioxidizing enzymes (superoxide dismutase, catalase, and glutathione peroxidae) are important enzymatic systems used to degrade hydrogen peroxide into water and oxygen, thereby lowering intracellular hydrogen peroxide levels. entomopathogenic fungi display increased activities of antioxidizing enzymes during growth and germination, which is necessary to counteract the hyperoxidant state produced by oxidative metabolism. we studied the influence of different carbon sources on antioxidizing enzy ...201323756951
an oil-based formulation of isaria fumosorosea blastospores for management of greenhouse whitefly trialeurodes vaporariorum (homoptera: aleyrodidae).to date, emphasis has been placed on studying the virulence of isaria fumosorosea conidia rather than the virulence of their blastospores. in the present study, a comparison was made of the efficacy of blastospores of i. fumosorosea (sfp-198) versus conidia against greenhouse whitefly (gwf) trialeurodes vaporarioum nymphs and thermotolerance at 50 °c. to improve thermotolerance and potency, the blastospores were suspended in corn oil with a wetting and an insect-filming agent.201323536463
control of key pecan insect pests using biorational pesticides.key pecan insect pests include the black pecan aphid, melanocallis caryaefoliae (davis), pecan weevil, curculio caryae (horn), and stink bugs (hemiptera: pentatomidae). alternative control tactics are needed for management of these pests in organic and conventional systems. our objective was to evaluate the potential utility of several alternative insecticides including three plant extract formulations, eucalyptus extract, citrus extract-8.92%, and citrus extract-19.4%, and two microbial insecti ...201323448039
compatibility of isaria fumosorosea (hypocreales: cordycipitaceae) blastospores with agricultural chemicals used for management of the asian citrus psyllid, diaphorina citri (hemiptera: liviidae).biorational insecticides are being increasingly emphasized for inclusion in integrated pest management programs for invasive insects. the entomopathogenic fungus, isaria fumosorosea, can be used to help manage the asian citrus psyllid with minimal impact on beneficial arthropods, but its effectiveness may be compromised by agrochemicals used to control concurrent arthropod pests and diseases. we evaluated the compatibility of i. fumosorosea blastospores with a range of spray oils and copper-base ...201326462531
performance of two isolates of isaria fumosorosea from hot climate zones in solid and submerged cultures and thermotolerance of their propagules.isaria fumosorosea frequently causes mycosis of agricultural pests in the hot semiarid and dry tropical regions of mexico. because temperature tolerance restricts the use of fungal biopesticides, we investigated two isolates from these areas for possible development into mycoinsecticides for use in hot weather agricultural zones. we studied the effects of culture system (solid or submerged cultures) and temperature on the fungal growth, extracellular enzyme production, pathogenicity, and thermot ...201323065378
characterization of paecilomycescinnamomeus from the camellia whitefly, aleurocanthus camelliae (hemiptera: aleyrodidae), infesting tea in japan.the whitefly, aleurocanthus camelliae kanmiya and kasai (hemiptera: aleyrodidae), is an invasive species in japan that was first discovered in 2004 on tea in kyoto. soon after its arrival epizootics of an entomopathogenic fungus were observed in populations of the whitefly in many tea-growing regions. here we identify this fungus as paecilomyces cinnamomeus (petch) samson and w. gams (hypocreales: clavicipitaceae) based on morphological characteristics and molecular analyses. this is the first r ...201222330093
laboratory evaluation of isaria fumosorosea against spodoptera littoralis.isaria fumosorosea (syn. paecilomyces fumosoroseus) is potentially useful for the biological control of economically important agricultural and forest insect pests. we evaluated efficacy of two strains of this entomopathogenic fungus against last instar larvae and pupae of egyptian cotton leafworm, spodoptera littoralis. the first strain was apopka 97 which is an active ingredient of commercial biopesticide preferal wg (biobest, belgium). the second strain was isolated from cameraria ohridella a ...201223885438
high resistance of isaria fumosorosea to carbendazim arises from the overexpression of an atp-binding cassette transporter (ift1) rather than tubulin mutation.probing possible mechanisms involved in the resistance of entomopathogenic fungus isaria fumosorosea to carbendazim fungicide.201222044666
retracted article: stress response of entomopathogenic fungus isaria fumosorosea to copper. 201121221837
complementary effect of phloxine b on the insecticidal efficacy of isaria fumosorosea sfp-198 wettable powder against greenhouse whitefly, trialeurodes vaporariorum west.insecticidal activities of five photoactive dyes against greenhouse whitefly (gwf), trialeurodes vaporariorum west., in tomatoes were investigated to improve the control efficacy of an entomopathogenic fungal product, isaria fumosorosea sfp-198 wettable powder (wp). azorubine, eosin b, erythrosine, brilliant green and phloxine b were used in this work, accompanying pyriproxyfen emulsifiable concentrate (ec) as a commercial standard for comparison.201020718026
persistence of isaria fumosorosea (hypocreales: cordycipitaceae) sfp-198 conidia in corn oil-based suspension.long-term persistence of entomopathogenic fungi as biopesticides is a major requirement for successful industrialization. corn oil carrier was superior in maintaining germination rates of isaria fumosorosea sfp-198 conidia during exposure to 50°c for 2 h, when compared with other oils, such as soybean oil, cottonseed oil, paraffin oil, and methyl oleate. the corn oil-based conidial suspension (91.6% germination) was also better in this regard than conidial powder (28.4% germination) after 50°c f ...201120571913
physiological effect of multi-walled carbon nanotubes (mwcnts) on conidia of the entomopathogenic fungus, paecilomyces fumosoroseus (deuteromycotina: hyphomycetes).the aim of the investigation was to assess the influence of commercial and carboxylated multi-walled carbon nanotubes (mwcnts) on conidia of the entomopathogenic fungus paecilomyces fumosoroseus. the commercial mwcnts had an external diameter of about 40-60 nm, a length of 300-600 nm, a specific density of 140 to 300 g/dm(3) and a carbon content of above 80%. carboxylated mwcnts were obtained by oxidizing commercial mwcnt by heating in hno(3), filtering, washing with water and drying. conidia af ...200920183518
production of thermotolerant entomopathogenic isaria fumosorosea sfp-198 conidia in corn-corn oil mixture.low thermotolerance of entomopathogenic fungi is a major impediment to long-term storage and effective application of these biopesticides under seasonal high temperatures. the effects of high temperatures on the viability of an entomopathogenic fungus, isaria fumosorosea sfp-198 (kctc 0499bp), produced on different substrates amended with various additives were explored. ground corn was found to be superior in producing the most thermotolerant conidia compared to yellow soybean, red kidney bean, ...201020157761
susceptibility of ceratitis capitata wiedemann (diptera: tephritidae) to entomopathogenic fungi.the mediterranean fruit fly ceratitis capitata (wiedemann) is one of the most serious pest of fruits in syria and present all year round. this fly is active on different host plants, such as mango, peach, fig, apple, pear, and citrus. the number of generations per year varies with local temperatures and host plant. the objective of this research was: to evaluate the pathogenicity of entomopathogenic fungi on adults of c. capitata under laboratory conditions. flies for experiments were obtained f ...200819226799
microsatellite variability in the entomopathogenic fungus paecilomyces fumosoroseus: genetic diversity and population structure.the hyphomycete paecilomyces fumosoroseus (pfr) is a geographically widespread fungus capable of infecting various insect hosts. the fungus has been used for the biological control of several important insect pests of agriculture. however knowledge of the fungus' genetic diversity and population structure is required for its sustainable use as a biological control agent. we investigated length and sequence polymorphisms of nine microsatellite loci for 33 pfr accessions sampled from various host ...200818268903
[intraspecific variations of paecilomyces fumosoroseus in temperature fitness and carbendazim resistance].a total of six wild strains of fungal biocontrol agent paecilomyces fumosoroseus were grown at 15 degrees c-35 degrees c to reveal their variations in colony growth, conidiation, and conidial viability. the results showed that the test three indices were the best at 25 degrees c, but differed significantly at different temperatures for a given strain or at a given temperature among test strains. the strains pfr116 and pfr6206 behaved better or more stably than other strains at 15 degrees c-35 de ...200717886653
virulence testing and extracellular subtilisin-like (pr1) and trypsin-like (pr2) activity during propagule production of paecilomyces fumosoroseus isolates from whiteflies (homoptera: aleyrodidae).to properly characterize several isolates of paecilomyces fumosoroseus, a fungal entomopathogen of whiteflies (homoptera: aleyrodidae) and other insect pests for biocontrol purposes, virulence towards trialeurodes vaporariorum, and subtilisin-like (pr1) and trypsin-like (pr2) protease activity during propagule production were investigated in monospore cultures (mcs). the virulence of three mcs towards second instar whiteflies was measured and expressed as lethal median concentration (lc50). numb ...200717592897
hydrophobic and electrostatic cell surface properties of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus.the physicochemical surface properties of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus were examined. contact angle measurements were performed on microbial lawns composed of blastospores of p. fumosoroseus to quantify their cell surface energy components. in addition, suspensions of the blastospores were characterized with the microbial adhesion to solvents assay. zeta potential measurements were used to quantify the surface charge and determine the zero potential of th ...200516388935
germination of conidia and blastospores of paecilomyces fumosoroseus on the cuticle of the silverleaf whitefly, bemisia argentifolii. 199916308757
the effect of selected allelochemicals on germination of conidia and blastospores and mycelial growth of the entomopathogenic fungus, paecilomyces fumosoroseus (deuteromycotina: hyphomycetes).selected allelochemicals that protect plants from invasion by plant pathogenic fungi were investigated for their activity against the entomopathogenic fungus, paecilomyces fumosoroseus. the alkaloids tomatine, solanine, and camptothecin; the furanocoumarin, xanthotoxin; and the phenolic, tannic acid were tested for their effects on germination of conidia and blastospores and growth of mycelia. the lc50 values (corresponding to 50% inhibition of germination) for tomatine, solanine, camptothecin, ...199816284855
impact of carbon and nitrogen nutrition on the quality, yield and composition of blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus.the impact of growing cultures of paecilomyces fumosoroseus in liquid media containing four combinations of glucose and casamino acids (8 g l(-1) or 80 g l(-1) glucose, 1.32 g l(-1) or 13.2 g l(-1) casamino acids) was evaluated, based on blastospore production, germination rate, viability after freeze-drying and short-term storage stability. when blastospores were produced using a high casamino acid concentration, blastospore yields and germination rates were significantly higher (13.2-18.5 x 10 ...200515887035
effect of different photoperiods on the growth, infectivity and colonization of trinidadian strains of paecilomyces fumosoroseus on the greenhouse whitefly, trialeurodes vaporariorum, using a glass slide bioassay.growth, infectivity and colonization rates for blastospores and conidia of trinidadian strains t, t10, and t11 of paecilomyces fumosoroseus (wize) brown and smith were assessed for activity against late fourth-instar nymphs of trialeurodes vaporariorum (westwood) (homoptera:aleyrodidae) under two different photoperiods (24 and 16 hour photophase). a glass-slide bioassay and a fungal development index, modified for both blastospores and conidia, were used to compare the development rates of the f ...200415861253
isolation of dipicolinic acid as an insecticidal toxin from paecilomyces fumosoroseus.several entomopathogenic fungi produce toxins that could be used as bioinsecticides in integrated pest management programs. paecilomyces fumosoroseus is currently used for the biological control of the whiteflies bemisia tabaci and b. argentifolii. supernatants from submerged batch culture, where the fungus produced abundant dispersed mycelium, conidia and blastospores, were toxic to the whitefly nymphs. the most abundant metabolite was purified by hplc and identified by mass spectrometry and nm ...200515696281
influence of temperature preferences of two paecilomyces fumosoroseus lineages on their co-infection order to clarify the epidemiological potential of entomopathogenic fungi for insect pest control, the role of the temperature as one environmental constraint was investigated on the pattern of co-infection of galleria mellonella by two distinct lineages of a hyphomycete, paecilomyces fumosoroseus. the distribution of conidial populations collected on cadavers of hosts co-infected under 20 regimes, ranging from 13 to 35 degrees c, was examined. the apparent temperature tolerance of both fungal ...200615579318
liquid-culture production of blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus using portable fermentation equipment.the production of fungal spores using on-site, non-sterile, portable fermentation equipment is technically constrained. very little information is available on the production requirements, such as medium concentration, inoculum stabilization, required fermentation times, and maintenance of axenic growth. in this study, we developed a two-part, liquid concentrate of the production medium that remains stable and soluble at room temperature. we also examined inoculum stability and showed that freez ...200415071762
effect of serial transfer of three strains of paecilomyces fumosoroseus on growth in vitro, virulence, and host specificity.serial passage of entomopathogenic hyphomycetes has been shown to alter virulence and host specificity. we evaluated virulence, host specificity, biomass production, conidiation, conidial germination, and a genetic fingerprint of 3 strains of paecilomyces fumosoroseus after passage in vitro or in vivo in diuraphis noxia or plutella xylostella. strain 4461 did not change in virulence toward d. noxia or p. xylostella after 30 passages in vitro nor after 15 passages in d. noxia. however, it lost vi ...200414992859
light-regulated asexual reproduction in paecilomyces fumosoroseus.the entomopathogenic fungus paecilomyces fumosoroseus has been successfully used in the control of several insect pests. asexually produced spores (conidia) are the means for dispersal and transmission of the entomopathogen; upon contact with the insect cuticle they germinate and penetrate the host. in model fungal systems it has been found that phototropism, resetting of the circadian rhythm, the induction of carotenogenesis and the development of reproductive structures are controlled by blue ...200414766909
combining azadirachtin and paecilomyces fumosoroseus (deuteromycotina: hyphomycetes) to control bemisia argentifolii (homoptera: aleyrodidae).both azadirachtin and paecilomyces fumosoroseus (wize) brown & smith have been used to control the whitefly bemisia argentifolii bellows & perring, but with only moderate effectiveness. azadirachtin is a botanical insecticide derived from the neem tree, and p. fumosoroseus is an entomopathogenic fungus. to test whether these two agents might be more effective for whitefly control if used together, different rates of each were combined in laboratory bioassays in factorial treatment. both tank mix ...200312650340
factors influencing the infectivity of isolates of paecilomyces fumosoroseus against diamondback moth, plutella xylostella.paecilomyces fumosoroseus isolate 1576 was isolated from an insect, but is avirulent against larvae of diamondback moth, plutella xylostella, and several other species. isolate 1576 grew faster and produced more conidia than isolate 4461 on potato dextrose agar. pregermination of conidia failed to increase the infectivity of isolate 1576, but the procedure did increase the infectivity of isolates 3682, 4461, and 4482. isolates 1576 and 4461 were both more infective when moisture was high during ...200111500091
influence of culture conditions on production and freeze-drying tolerance of paecilomyces fumosoroseus blastospores.with the goal of developing a defined medium for the production of desiccation-tolerant blastospores of the bioinsecticidal fungus paecilomyces fumosoroseus, we evaluated the impact of various media components such as amino acids, carbohydrates, trace metals and vitamins on hyphal growth and sporulation of p. fumosoroseus cultures and on the freeze-drying tolerance of blastospores produced under these conditions. a comparison of 13 amino acids as sole nitrogen sources showed that glutamate, aspa ...199910510486
use of the green fluorescent protein for investigations of paecilomyces fumosoroseus in insect hosts.the green fluorescent protein (gfp) from the jellyfish aequorea victoria has been expressed in a variety of prokaryotic and eukaryotic organisms and has been used extensively as a marker in the study of host-pathogen interactions. we have expressed gfp in the entomopathogenic fungus paecilomyces fumosoroseus through co-transformation with a vector that confers resistance to glufosinate ammonium. all cell types express gfp and were readily detected by fluorescence microscopy. no correlation was o ...199910486232
pathogenicity of paecilomyces fumosoroseus isolates to diamondback moth, plutella xylostella: correlation with spore size, germination speed, and attachment to cuticle.infectivity to larvae of the diamondback moth, plutella xylostella, was compared among eight paecilomyces fumosoroseus isolates. isolate infectivity was assessed for correlation with spore length and germination speed. four isolates applied to p. xylostella cuticle were also compared for number of spores remaining on the cuticle after washing and for percentage germination after 36 h. infection of larvae inoculated with the different isolates at an average dosage of 4000 conidia/cm2 ranged from ...199910222189
relative efficacy of blastospores and aerial conidia of paecilomyces fumosoroseus against the russian wheat aphid. 19989709022
high frequency gene transfer by microprojectile bombardment of intact conidia from the entomopathogenic fungus paecilomyces fumosoroseus.two different methods, (i) peg and (ii) biolistic, were employed to transform protoplasts and conidia of paecilomyces fumosoroseus using hygromycin resistance as selectable marker. transformation frequencies varied from 1.9 to 2.5 transformants microgram-1 of dna by the peg method, and from 33 to 153 transformants microgram-1 of dna by the biolistic procedure.19979368365
in vitro effects of secondary plant compounds on germination of blastospores of the entomopathogenic fungus paecilomyces fumosoroseus (deuteromycotina: hyphomycetes)seven secondary plant compounds (catechol, chlorogenic acid, gallic acid, salicylic acid, saponin, sinigrin, and tannic acid) mixed with noble agar at three concentrations (100, 500, and 1000 ppm) were tested for their effects on germination of blastospores of the fungal entomopathogen paecilomyces fumosoroseus. with individual allelochemicals incorporated at 100 ppm in noble agar, significant differences in time to 95% germination were found between two allelochemicals (catechol and salicylic a ...19979367728
genetic variability of paecilomyces fumosoroseus isolates revealed by molecular markers.paecilomyces fumosoroseus (deuteromycotina:hyphomycetes) is a fungus that is potentially useful for the bio-control of economically important agricultural pests, such as whitefly (bemisia tabaci). arbitrarily primed pcr and pcr with trna consensus primers were used to analyze genetic variability among 27 p. fumosoroseus isolates, 15 of which came from the same host, b. tabaci, one p. lilacinus isolate, used as an outgroup, 9 previously unidentified paecilomyces isolates. fifteen 10-mer oligonucl ...19957745282
beauverolides l and la from beauveria tenella and paecilomyces beauverolides l and la were isolated and identified from the entomopathogenic fungi, beauveria tenella and paecilomyces fumosoroseus. their structures, cyclo-[3-hydroxy-4-methyldecanoyl-l-phenylalanyl-l-alanyl-d-leucyl ], and cyclo-[3-hydroxy-4-methyldecanoyl-l-phenylalanyl-l-alanyl-d-allo-i soleucyl] were deduced from hplc and gc-mass spectrometric analyses of their hydrolysates and nmr and mass spectral data.19947765754
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