| experimental infection of lambs with an adenovirus isolated from sheep and related to bovine adenovirus type 2. i. virological studies. | | 1975 | 171937 |
| experimental infection of calves with an adenovirus isolated from sheep and related to bovine adenovirus type 2. ii. pathological and histopathological studies. | | 1978 | 207053 |
| multiple enlargements in the right inverted terminal repeat of the dna of canine adenovirus type 2. | the manhattan strain of canine adenovirus type 2 (cav 2) was examined. restriction endonuclease analysis and blot hybridization experiments revealed the heterogeneity of the viral dna. at least 9 unequally expanded species of the viral genome have been recognized. this diversity is caused by different enlargements in the right inverted terminal repeat (itr) of the virus. the differences between the individual enlargements were shown to be the different multiples of 150 base pairs. relatedness of ... | 1992 | 1307439 |
| sequence analysis of bovine adenovirus type 2 early region 1 and pix gene. | bovine adenoviruses (bavs) cause both respiratory and/or enteral diseases in cattle and are widespread throughout the world. we have sequenced the extreme left end (0 to 12.2 mu) of the bav2 genome. partial analysis of the nucleotide sequence revealed 12 potential open reading frames (orfs) which could encode for polypeptides of 50 or more amino acids. these orfs were compared to those of the early region 1 (e1) proteins and hexon-associated protein ix (pix) from other adenoviruses (ads). the 4 ... | 1994 | 7698885 |
| occurrence of some viruses and bacteria involved in respiratory diseases of ruminants in hungary. | serological surveys, virological and bacteriological investigations were carried out on large populations of lambs and calves kept in intensive fattening units on hungarian large-scale farms, in order to study the occurrence of viruses and bacteria involved in the respiratory disease complex. the investigations were carried out in stocks where 5-20% of the young animals succumbed to acute or chronic pneumonia every year. serological surveys revealed that seropositivity to bovine adenovirus type ... | 1998 | 9713141 |
| dna sequencing and phylogenetic analysis of the protease gene of ovine adenovirus 3 suggest that adenoviruses of sheep belong to two different genera. | until now, the only published ovine adenovirus dna sequence was the complete genome of ovine adenovirus isolate 287 (oav287) which, compared to other mammalian adenoviruses, possesses strikingly unique genomic organisation and should properly be classified into a new adenovirus genus. the protease gene sequence of ovine adenovirus type 3 (oadv-3) was determined and analysed. the results of phylogenetic analysis of the 205 residue long protein demonstrated that oadv-3 belongs to the genus mastade ... | 2000 | 10653919 |
| molecular comparison of the fibre proteins of bovine adenovirus subtype a and subtype b. | the fibre gene of the bovine adenovirus type 2 (badv2) subtype b was prepared for sequencing by using cloning, sub-cloning and pcr amplification techniques. the nucleotide sequence of the total fibre gene was determined, and it was found to consist of 1,647 nucleotides, coding for a polypeptide of 549 amino acids. the fibre gene regions of badv2 a and b subtypes were aligned. the nucleotide identity of the total fibre gene was found to be 60.5%; however, the homology showed great differences in ... | 2003 | 12837557 |
| pasteurella haemolytica complicated respiratory infections in sheep and goats. | respiratory infections which commonly occur in sheep and goats often result from adverse physical and physiological stress combined with viral and bacterial infections. inevitably, pasteurella haemolytica pneumonia occurs as a result of these interactions. in this review, we present recent advances in research on the complex etiology of pneumonia involving p. haemolytica. initially stress, induced by factors such as heat, overcrowding, exposure to inclement weather, poor ventilation, handling an ... | 1998 | 9689740 |
| cloning and sequencing of the bovine adenovirus type 2 early region 4. | bovine adenovirus type 2 (bav2) is a medium size double-stranded dna virus which infects both bovine and ovine species, resulting in mild respiratory and gastrointestinal disorders. to better understand the virus and its growth characteristics in madin-darby bovine kidney (mdbk) cells, we have cloned and sequenced the extreme right-end segment of the bav2 genome (90.5-100 map units). analysis of the nucleotide sequence revealed 40 potential open reading frames (orfs) with coding capacity for pol ... | 1997 | 9055813 |
| intertype specific epitopes on the hexons of adenovirus types 1, 2, 5 and 6. | the intertype specificities of the epitopes present on the surface of the hexon types belonging to human adenovirus (ad h) subgenus c were studied. the reactivity pattern (rp) and the titres of ascitic fluids containing monoclonal antibodies (mabs) were determined in elisa. from three panels of mabs 38 directed against ad h1, 17 directed against ad h 35 and 6 mabs directed against bovine adenovirus type 2 were studied. twelve of the mabs proved to be genus or type specific. the rest of the mabs ... | 1996 | 8806946 |
| experimental infection of weaned calves and lambs with two strains of bovine adenovirus type 2. | the pathogenicity of two genetically different strains of bovine adenovirus type 2 (bav-2) was tested in a double cross experimental infection of weaned lambs and calves. one of the virus strains was isolated from cattle and represented bav-2 subgroup "a", while the other was isolated from sheep and belonged to subgroup "b" (belák et al., 1983). the two virus strains caused infection both in lambs and calves; the viruses were repeatedly reisolated and definite seroconversion was found in all fou ... | 1993 | 8391744 |
| subcloning and restriction enzyme mapping of bovine adenovirus type 2. | bovine adenoviruses (bavs), causing both respiratory and/or enteral diseases in cattle, have been isolated in many countries all over the world. in this study we report on the molecular cloning of the internal ecori fragments spanning 20.6-90.5%, and the internal sali fragments spanning 3.1-65.2% of the bav type 2 (bav2; strain no. 19) genome into the plasmid vector puc19. moreover, the subcloning of the bav2 genome facilitated the construction of a detailed physical restriction endonuclease map ... | 1993 | 8294184 |
| sequence analysis of the putative e3 region of bovine adenovirus type 2. | adenoviruses are nonenveloped icosahedral-shaped particles. the double-stranded viral dna genome contains four major early transcription units, designated e1 (a and b), e2 (a and b), e3 and e4, which are expressed in a regulated manner soon after infection. the gene products of the region e3, shown to be nonessential for viral replication in vitro, are believed to be involved in counteracting host immunosurveillance. human adenovirus type 5 dna sequences of transcription units l4 and l5 adjacent ... | 1994 | 7698883 |
| antigenic homogeneity among the adenovirus hexon types of subgenus c. | antigenic relationships of hexons of human adenovirus (ad h) types 1, 2, 5 and 6 of subgenus c were studied with 61 monoclonal antibodies (mabs) raised against human ad h1, ad h35 and bovine adenovirus 2. the reactivity pattern (rp) and the titers of the mabs were determined in indirect elisa. in previous experiments with hexons of different subgenera 49 mabs displayed numerous different intertype specificities besides genus specific and type specific ones. with the four hexon types of subgenus ... | 1995 | 7646361 |
| dna structure, and hemagglutination properties of bovine adenovirus type 2 strains which bypass species specificity. | bovine adenovirus type 2 (ad bos 2) strains were examined which had been isolated during natural outbreaks among calves, and lambs in hungary [belák, s., pálfi, v.: arch. ges. virusforsch. 46,366-369 (1974)]. differences were detected in hemagglutination properties, and in the restriction site maps of the dna, which seem to be sufficient to group isolates of ad bos 2 into two subtypes (subspecies). some of the strains, recovered from cattle including prototype strain no. 19 are suggested to be s ... | 1983 | 6314935 |
| subtypes of bovine adenovirus type 2 exhibit major differences in region e3. | the genomes of two adenovirus type 2 strains which were isolated from different hosts have been investigated. one of these strains designated ort-111 was originally isolated from a lamb in hungary during an outbreak of pneumoenteritis. this isolate was typed as bovine adenovirus type 2 (ad bos 2) in a neutralization assay. the genome of ort-111 was compared to that of the prototype strain of ad bos 2, a virus which exclusively has been isolated from cattle. electron microscopic heteroduplex anal ... | 1986 | 3739229 |
| antigenic structure of mammalian adenoviruses studied by monoclonal antibodies against bovine adenovirus type 2. | the cross-reactivity pattern of eight monoclonal antibodies (mabs) raised against bovine adenovirus type 2 (bav2) hexon was studied by indirect elisa using seven different adenoviruses. all of the eight mabs reacted with the two bav2 subtypes (a and b), while the other adenoviruses, human adenovirus type 1 (hav1), ovine adenovirus type 3 (oav3), bav1, bav4 and bav8 gave different combinations of positive reactions. based on the results of cross-reactivity there is a close antigenic relationship ... | 1988 | 2469284 |
| antigenic relationship between human and animal adenovirus hexons determined by means of monoclonal antibodies directed against bovine adenovirus type 2 hexon. | eight monoclonal antibodies (mabs) directed against bovine adenovirus (bav) 2 subtype b hexon were studied with 12 different hexon types of human adenoviruses (av) belonging to 5 different subgenera using indirect elisa, passive hemagglutination (ha), and gel diffusion assays. two hexon types of animal origin (bav3 and sav16) were investigated, too. the reactivity of the mab iv.f3 was the broadest, i.e. in elisa and ha experiments it reacted with all hexon types studied. based on these results a ... | 1988 | 2455494 |
| replication of ovine adenoviruses. | the replication of ovine adenoviruses shows intertypic and intratypic variations, e.g. the extracellular and intracellular virus yield of ort/111, a strain related to bovine adenovirus type 2, reached its peak 40 to 46 hr postinfection, in contrast to gy/14, a strain classified as ovine adenovirus type 1, which required 52 to 58 hr to reach the highest yield. the replication cycle was not appreciably influenced either by rolling of the tube cultures or the age (between 0 and 7 days) of the suppo ... | 1978 | 382754 |
| experimental infection of calves with an adenovirus isolated from sheep and related to bovine adenovirus type 2 i. clinical and virological studies. | colostrum deprived calves were experimentally infected with an adenovirus isolated from sheep and related to bovine adenovirus type 2. the calves showed respiratory symptoms and mild diarrhoea from the third day after infection. laboratory tests revealed the development of leucopenia, lymphopenia, a drop of the ph of the urine and the appearance of pathological changes in the urine. the animals shed the virus in their nasal discharge, faeces and urine. comparing the clinical and virological find ... | 1977 | 199002 |
| characterisation of the fiber gene and partial sequence of the early region 4 of bovine adenovirus 2 (short communication). | the full sequence of the fiber gene and partial sequence of the putative 17 kd protein gene of bovine adenovirus-2 (badv-2) were determined. the size of the fiber gene of badv-2 proved to be 561 amino acids, of which the amino acids 37 to 385 form a typical shaft domain of 22 repetitive motifs. on the complementary strand, a gene homologous to the 17 kd protein coded in the e4 region of several human adenoviruses was found. the sequence analysis seems to confirm the presence of an intron in the ... | 2001 | 11402654 |
| identification and sequence analysis of the core protein genes of bovine adenovirus 2. | the dna sequence of the genome of bovine adenovirus type 2 (badv-2) was determined between map units 42.5 and 50. by sequence analysis and homology search, the genes of five structural proteins were identified within this region: the penton base protein (iii; partial sequence), the major core protein precursor (pvii), the minor core protein (v), the mu core protein precursor (px) and the hexon associated protein precursor (pvi; partial sequence). the putative polypeptides were compared to their ... | 2000 | 11074122 |