| purification of oat necrotic mottle virus with silver nitrate as clarifying agent. | | 1971 | 5165853 |
| a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family. | a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ... | 2001 | 11402861 |
| phylogenetic relationships, strain diversity and biogeography of tritimoviruses. | north american and eurasian isolates of wheat streak mosaic virus (wsmv; genus tritimovirus) and oat necrotic mottle virus (onmv; genus rymovirus) were examined. nine wsmv isolates differentially infected oat, barley, inbred maize line sdp2 and sorghum line ks56. the wsmv isolates clustered into groups based on phylogenetic analyses of the capsid protein (cp) cistron and flanking regions. wsmv isolates from the united states (us) and turkey were closely related, suggesting recent movement betwee ... | 2002 | 11907340 |
| complete nucleotide sequence of oat necrotic mottle virus: a distinct tritimovirus species (family potyviridae) most closely related to wheat streak mosaic virus. | the rna genome (9346 nucleotides) of oat necrotic mottle virus (onmv) was cloned and sequenced. complete genome comparisons indicated that onmv, currently classified as a rymovirus, was most closely related ( approximately 73% nucleotide, approximately 79% amino acid identity) to the tritimovirus wheat streak mosaic virus (wsmv). onmv encoded a single polyprotein, with proteinase cleavage sites very similar to those of wsmv. pairwise comparison of onmv and wsmv cistrons revealed that p3 was most ... | 2004 | 14991449 |
| functional replacement of wheat streak mosaic virus hc-pro with the corresponding cistron from a diverse array of viruses in the family potyviridae. | helper component-proteinase (hc-pro) of wheat streak mosaic virus strain sidney 81 (wsmv-sidney 81) was systematically replaced with the corresponding cistron derived from four strains of wsmv (type, tk1, cz, and el batán 3), the tritimovirus oat necrotic mottle virus (onmv), the rymoviruses agropyron mosaic virus (agmv) and hordeum mosaic virus (homv), or the potyviruses tobacco etch virus (tev) and turnip mosaic virus (tumv). these hc-pro proteins varied in amino acid sequence identity shared ... | 2004 | 15193921 |
| plant virus hc-pro is a determinant of eriophyid mite transmission. | the eriophyid mite transmitted wheat streak mosaic virus (wsmv; genus tritimovirus, family potyviridae) shares a common genome organization with aphid transmitted species of the genus potyvirus. although both tritimoviruses and potyviruses encode helper component-proteinase (hc-pro) homologues (required for nonpersistent aphid transmission of potyviruses), sequence conservation is low (amino acid identity, approximately 16%), and a role for hc-pro in semipersistent transmission of wsmv by the wh ... | 2005 | 15994799 |
| analyses of the complete sequence of the genome of wheat eqlid mosaic virus, a novel species in the genus tritimovirus. | the complete nucleotide sequence of the genome of wheat eqlid mosaic virus (weqmv) (excluding the poly a tail) comprised 9636 nucleotides including 5' and 3' noncoding regions of 137 and 172 nt, respectively. it contained a single orf coding for a polyprotein of 3,109 amino acid residues and had a deduced genome organization typical of members of the family potyviridae and with proteinase cleavage sites very similar to those of the members of the genus tritimovirus. pairwise and multiple alignme ... | 2008 | 18648923 |