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diagnosis of intestinal parasites in a rural community of venezuela: advantages and disadvantages of using microscopy or rt-pcr.a cross-sectional study was carried out to determine the prevalence and diagnostic performance of microscopy and real time pcr (rt-pcr) for 14 intestinal parasites in a venezuelan rural community with a long history of persistent intestinal parasitic infections despite the implementation of regular anthelminthic treatments. a total of 228 participants were included in this study. a multiplex rt-pcr was used for the detection of dientamoeba fragilis, giardia intestinalis, cryptosporidium sp. and ...201728007484
a decade of intestinal protozoan epidemiology among settled immigrants in qatar.the world health organization estimates that about 3.5 billion people worldwide are affected by intestinal parasitic infections. reports have already emphasized the role of immigrants in outbreaks of parasitic diseases in industrialized countries. with the mass influx of immigrants to qatar, patent intestinal parasitic infections have been observed. herein, the prevalence of intestinal protozoan infections was analysed in 29,286 records of subjects referred for stool examination at the hamad med ...201627496143
detection of parasites in children with chronic diarrhea.the aim of this study was to investigate the frequency of intestinal parasites in patients with chronic diarrhea and clarify the importance of these parasitic pathogens in such cases. a total of 60 pediatric patients with chronic diarrhea between june 2012 and october 2014 were enrolled in the study. out of 60 stool samples, five were positive for giardia lamblia, two, dientamoeba fragilis, and one, blastocystis hominis. one stool sample was positive for entamoeba hartmanni and b. hominis, anoth ...201627322863
molecular identification of entamoeba species in savanna woodland chimpanzees (pan troglodytes schweinfurthii).to address the molecular diversity and occurrence of pathogenic species of the genus entamoeba spp. in wild non-human primates (nhp) we conducted molecular-phylogenetic analyses on entamoeba from wild chimpanzees living in the issa valley, tanzania. we compared the sensitivity of molecular [using a genus-specific polymerase chain reaction (pcr)] and coproscopic detection (merthiolate-iodine-formaldehyde concentration) of entamoeba spp. we identified entamoeba spp. in 72 chimpanzee fecal samples ...201626935395
frequency and molecular characterisation of entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, and entamoeba hartmanni in the context of water scarcity in northeastern brazil.this study aimed to estimate the frequency, associated factors, and molecular characterisation of entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, andentamoeba hartmanni infections. we performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. faecal samples were processed through flotation and centrifugation methods.e. histolytica, e. dispar, e. moshkovskii, and e. hartmanni were identified by nested-polymerase chain reaction (pcr). th ...201626841049
intestinal parasites in diabetic patients in sohag university hospitals, egypt.intestinal parasites usually create benign diseases, though they may induce complications with high morbidity and mortality to the immunocompromised, including diabetic patients. the study detected the prevalence of intestinal parasitic infections in diabetic patients, comparing to non-diabetic controls and other parameters. a total of 100 fecal samples were collected from diabetic patients at the outpatient clinic of sohag university hospitals and another 100 from cross matched controls. the sa ...201526485865
diversity and prevalence of gastrointestinal parasites in seven non-human primates of the taï national park, côte d'ivoire.parasites and infectious diseases are well-known threats to primate populations. the main objective of this study was to provide baseline data on fecal parasites in the cercopithecid monkeys inhabiting côte d'ivoire's taï national park. seven of eight cercopithecid species present in the park were sampled: cercopithecus diana, cercopithecus campbelli, cercopithecus petaurista, procolobus badius, procolobus verus, colobus polykomos, and cercocebus atys. we collected 3142 monkey stool samples betw ...201525619957
differential diagnosis of entamoeba spp. in clinical stool samples using sybr green real-time polymerase chain reaction.amoebiasis, a disease caused by entamoeba histolytica, is usually diagnosed by microscopic examination, which does not differentiate the morphologically identical species of the e. histolytica/e. dispar complex. furthermore, morphologically similar species such as entamoeba hartmanni contribute to misidentification. therefore, there is a need for more sensitive and specific methods. this study standardized a multiplex real-time pcr system for e. histolytica and e. dispar and a single real-time p ...201424693242
frequency of amoebiasis and other intestinal parasitoses in a settlement in ilhéus city, state of bahia, brazil.this study evaluated the frequency of intestinal parasites, emphasizing the identification and differentiation of entamoeba spp.201424553799
molecular detection of eukaryotes in a single human stool sample from senegal.microbial eukaryotes represent an important component of the human gut microbiome, with different beneficial or harmful roles; some species are commensal or mutualistic, whereas others are opportunistic or parasitic. the diversity of eukaryotes inhabiting humans remains relatively unexplored because of either the low abundance of these organisms in human gut or because they have received limited attention from a whole-community perspective.201222808282
[the distribution of intestinal parasites in people admitted to the yüzüncü yıl university parasitology laboratory of health research and training hospital, in 2009].this study was performed to present the distribution of intestinal parasites in parients admitted to the parasitology laboratory of the health research and training hospital of yüzüncü yıl university in 2009.201222801916
[frequency of intestinal parasites among administrators and workers in sanitary and non-sanitary institutions.]objective: transmission of parasites generally occurs through fecal-oral means directly from human to human or through receiving eggs and cysts by means of nourishment. the aim of the study was to investigate the frequency of intestinal parasites among administrators and workers in sanitary and non-sanitary institutions. methods: stool specimens were examined using native-lugol, trichrome and acid-fast stains methods. results: 23.7% of the 2443 fecal specimens were found to be positive. the freq ...201121618189
increased sampling reveals novel lineages of entamoeba: consequences of genetic diversity and host specificity for taxonomy and molecular detection.to expand the representation for phylogenetic analysis, ten additional complete entamoeba small-subunit rrna gene sequences were obtained from humans, non-human primates, cattle and a tortoise. for some novel sequences no corresponding morphological data were available, and we suggest that these organisms should be referred to as ribosomal lineages (rl) rather than being assigned species names at present. to investigate genetic diversity and host specificity of selected entamoeba species, a tota ...201121295520
[investigation of intestinal parasites in students of mustafa cengiz primary school in van].intestinal parasites still maintain as a major public health problem in our country. in this study, we aimed to investigate the distribution of intestinal parasites in 1st and 2nd grade students of mustafa cengiz primary school, aged between 7-9 and to contribute to the parasitological data of our province. for this purpose, stool examinations of a total of 195 students, including 82 boys and 113 girls, were performed. the results of the microscopic analysis of stool samples revealed one or more ...201020954118
[intestinal parasitoses detected in primary schoolchildren in the van province].this study was carried out on school children in primary schools in the van province in order to investigate the distribution of intestinal parasitoses. the study was conducted in 7-15 year-old primary schoolchildren during the years from 2003-2005. stool specimens were taken from 2975 school children; of these, 1289 were female and 1686, male. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of the health research and training ...200920101580
absence of opportunistic parasitic infestations in children living with hiv/aids in children's homes in jamaica: pilot investigations.many children living with hiv/aids in developing countries are infected with intestinal parasites. these infections add unnecessary morbidity to children already suffering the clinical insult of living with hiv/aids.200819583124
enteroparasites and commensals among children in four peripheral districts of uberlândia, state of minas gerais.the aim of this study was to determine the occurrence of intestinal parasites and commensals among children in four peripheral districts located in the northern, southern, eastern and western sectors of uberlândia, minas gerais, using the baermann methods as modified by moraes and lutz. out of 160 individuals studied, 93 (58.1% ci: 50.4-65.7) were infected, distributed among the sectors as follows: northern (72.5%), southern (47.5%), eastern (57.5%) and western (55%). the positive findings accor ...200819142436
[the prevalence of intestinal parasites in children brought to the kars maternal and children's hospital with complaints of gastrointestinal symptoms].this study was carried out to determine the prevalence of intestinal parasites in 2-6 year-old children who were brought to kars maternal and children's hospital with complaints of gastrointestinal symptoms during march-june 2007. fecal samples were taken from children and brought to the parasitology laboratory of the faculty of veterinary medicine to be examined for intestinal parasites. fecal samples were examined by centrifugal formalin ether, zinc-sulphate floatation, and modified acid fast ...200818985583
[prevalence of intestinal parasites among workers in food sector in van region].stool and cellophane tape specimens were taken for parasitological examination from 739 people who work in food sector and applied to the public health lab of the van health administration for porter examination. parasites were determined at 131 people (17.71%) of 739 worker whom samples were investigated. ninety-five people had one, 30 people had two, 5 people had three and one person had four parasite species. parasites determined in the study were 19.08% helminthes and 80.91% protozoon. in th ...200718224624
intestinal parasitic infection among children and neonatus admitted to ibn-sina hospital, sirt, libya.a total of 350 stool samples from 196 males and 154 female children and neonatus admitted in ibn-sina hospital, sirt, were examined from june 2001 to may 2002, to determine the prevalence of intestinal parasites. intestinal parasitic infections were identified in 196 (56%) of children and neonates. no intestinal helminthic parasites were detected but 13 intestinal protozoan parasites were detected. the most prevalent protozoan was entamoeba histolytica /e. dispar (36.57%); blastocystis hominis ( ...200717985574
laboratory diagnostic techniques for entamoeba species.the genus entamoeba contains many species, six of which (entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, entamoeba polecki, entamoeba coli, and entamoeba hartmanni) reside in the human intestinal lumen. entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. although recent studies highlight the recovery of e. dispar and e. moshkovskii from patients with gastrointestinal symptoms, there is still no convin ...200717630338
prevalence of enteric protozoa in human immunodeficiency virus (hiv)-positive and hiv-negative men who have sex with men from sydney, australia.a prospective, comparative study of the prevalence of enteric protozoa was determined among human immunodeficiency virus (hiv)- positive and hiv-negative men who have sex with men (msm) in sydney, australia. a total of 1,868 patients submitted stool specimens; 1,246 were from msm (628 hiv positive and 618 hiv positive) and 622 from non-msm were examined over a 36-month period. a total of 651 (52.2%) stool specimens from msm were positive for protozoa compared with 85 (13%) from non-msm. there wa ...200717360882
[enteric parasites and aids in haiti: utility of detection and treatment of intestinal parasites in family members].intestinal parasites and human immunodeficiency virus (hiv) are major health problems in haiti. both entities are known to interact strongly with cell-mediated immunity. the purpose of this study undertaken in port-au-prince, haiti was to evaluate the risk of enteric parasite transmission between hiv-infected patients and family members. routine examination of stool specimens for parasites was conducted in 90 hiv-infected undergoing treatment for intestinal disorders due mainly to cryptosporidiu ...200617201290
[incidence of intestinal parasites in municipal sanitary workers in malatya].the incidence of intestinal parasites is closely related to such factors as the socio-economic level of the society, nutritional and hygienic habits, climate, environmental conditions, infrastructure and degree of literacy. in this study, the municipal sanitary workers who are regarded as a high risk group in malatya were examined for intestinal parasites. cellophane slides and fecal samples from 241 workers were examined and intestinal parasites were found in 93 (39.0%). the most common parasit ...200617160847
[investigation of intestinal parasites in residents of the kayseri karpuzsekisi basin].in this study, we collected stool and cellophane tape samples from residents in four villages located in the kayseri-karpuzsekisi basin in order to investigate the distribution of intestinal parasites. a total of 240 samples collected from hanyeri (40), sariküklü (20), dokuzpinar (80) and karpuzsekisi (120) were investigated. the parasites detected were as follows: blastocystis hominis, 82 (34.16%); enterobius vermicularis, 37 (15.41%); entamoeba coli 26, (10.83%); entamoeba hartmanni, 10 (4.16% ...200617160846
[distribution of intestinal parasites among patients who presented at the department of parasitology of the erciyes university medical school.]in this study carried out from 2000-2004, a total of 34,883 stool samples were examined using native-lugol and flotation/sedimentation methods and 9,879 cellophane tape preparations were examined directly. intestinal parasites were found in 9,704 (27.8%) of the specimens. the parasites that were found and their prevalence is as follows: blastocystis hominis, 6,723 (19.3%); entamoeba coli, 1,007 (2.9%); giardia intestinalis, 892 (2.6%); entamoeba histolytica/e. dispar, 798 (2.3%); endolimax nana, ...200517124684
prevalence of enteric parasites in pet macaques in sulawesi, indonesia.on the indonesian island of sulawesi, nonhuman primate pets come into frequent contact with humans, presenting the possibility of zoonotic and anthropozoonotic disease transmission. we collected fecal samples from 88 pet macaques representing six of the seven macaque species currently recognized as endemic to sulawesi (macaca nigra, m. nigrescens, m. hecki, m. tonkeana, m. maura, and m. ochreata) as well as two non-endemic species (m. fascicularis and m. nemestrina) in order to determine the pre ...200414983465
detection and identification of entamoeba species in stool samples by a reverse line hybridization assay.classically, detection of entamoeba histolytica is performed by microscopic examination for characteristic cysts and/or trophozoites in fecal preparations. differentiation of e. histolytica cysts and those of nonpathogenic amoebic species is made on the basis of the appearance and the size of the cysts. however, by classical means objective tools for confirmation and quality control do not exist. therefore, a reverse line blot hybridization assay was developed to detect a variety of entamoeba sp ...200314605136
[entamoeba histolytica, schaudinn, 1903 and entamoeba hartmanni (prowazek, 1912)]. 196314338305
identification of entamoeba hartmanni trophozoites from nuclear structure. 196414114353
increase in size of entamoeba hartmanni trophozoites cultured on an enriched medium. 196314070761
size and nuclear morphology of entamoeba histolytica and entamoeba hartmanni trophozoites in cultures and in man. 196313948419
microfluorimetric evidence of antigenic difference between entamoeba histolytica and entamoeba hartmanni. 195913850706
morphological differentiation of entamoeba hartmanni and e. polecki from e. histolytica. 195913806300
[observations on entamoeba hartmanni (prowazek, 1912)]. 195713503514
prevalence of endoparasitic infection in children and its relation with cholera prevention efforts in mexico.to investigate whether increased knowledge and use of public health measures promoted for cholera prevention is reflected in lower prevalence of parasitic infection in households in a community in the state of tamaulipas, mexico, that is close to the border with the united states of america.200312952605
gastrointestinal parasites of mountain gorillas (gorilla gorilla beringei) in the parc national des volcans, rwanda.ninety-eight fecal samples were collected from 74 free-living mountain gorillas (gorilla gorilla beringei) from the parc national des volcans, rwanda, between july 1995 and january 1997 and examined for parasites by sheather's sugar and zinc sulfate flotation methods, trichrome staining, and larval cultures. all samples contained at least one parasite. seventeen endoparasites were identified, including eight protozoa, seven nematodes, one cestode, and one trematode. two species of arthropod mite ...200011237138
multiyear prospective study of intestinal parasitism in a cohort of peace corps volunteers in guatemala.we conducted a prospective, longitudinal study in a cohort of 36 peace corps volunteers (pcvs) in guatemala to study the incidence and natural history of intestinal parasitic infections during the pcvs' >2-year overseas stay. pcvs collected stool specimens at least monthly and when ill with gastrointestinal symptoms. of the 1,168 specimens tested, 453 (38.8%) were positive for at least one parasite and 48 (4.1%) were positive for a pathogenic parasite. a median interval of 187 days (range, 14 to ...200111136744
inhibition of enteric parasites by emulsified oil of oregano in vivo.oil of mediterranean oregano oreganum vulgare was orally administered to 14 adult patients whose stools tested positive for enteric parasites, blastocystis hominis, entamoeba hartmanni and endolimax nana. after 6 weeks of supplementation with 600 mg emulsified oil of oregano daily, there was complete disappearance of entamoeba hartmanni (four cases), endolimax nana (one case), and blastocystis hominis in eight cases. also, blastocystis hominis scores declined in three additional cases. gastroint ...200010815019
entamoeba hartmanni: a new causative agent in the pathogenesis of reactive arthritis? 19989672998
evaluation of intestinal protozoan morphology in human fecal specimens preserved in ecofix: comparison of wheatley's trichrome stain and ecostain.as a result of disposal problems related to the use of mercury compounds, many laboratories have switched from mercuric chloride-based schaudinn's and polyvinyl alcohol (pva) stool preservatives to other, non-mercury-based preservatives. a comparison of organism recoveries and morphologies of the intestinal protozoa was undertaken with pva containing the ecofix zinc-based schaudinn's preservative (meridian diagnostics, inc.); both wheatley's modification of gomori's trichrome stain (wt) and ecos ...19989650947
[intestinal parasitic infections in serbia].to determine the public health significance of intestinal parasitism in serbia today, systematic parasitologic examination of 16 regions (kragujevac, luchani, zhagubica, bor, sjenica, novi pazar, valjevo, aleksandrovac, pirot, bosilegrad, ivanjica, golubac, uzhice, kladovo, negotin, beograd) in central serbia were carried out over the period 1984-1993. the study involved a total of 5981 schoolchildren (2887 f, 3094 m), 7-11 years old representing 10% of the total age-matched population (n = 58,2 ...19989525075
[epidemiology of children's intestinal parasitism in the guadalquivir valley, spain].intestinal parasitism represents a public health problem that should be periodically assessed in each region. in the present paper, a study about prevalence of intestinal parasites, has been carried out in children from the natural region of the guadalquivir valley.19979477709
[prevalence of intestinal parasites in a student population].we performed a transversal descriptive study to know the prevalence of intestinal parasites of our children's community. a 891 fecal sample analyzed from an equal number of apparently healthy children, whose ages were between 5-14 years old, randomly selected by stratified sampling by years old.19958555305
the prevalence of giardia and other intestinal parasites in children, dogs and cats from aboriginal communities in the kimberley.to determine the prevalence of giardia duodenalis and other intestinal parasites in children, dogs and cats from aboriginal communities in the west kimberley region of western australia.19938450779
production and characterization of monoclonal antibodies against a highly immunogenic fraction of entamoeba histolytica (nih:200) and their application in the detection of current amoebic infection.six monoclonal antibodies (mabs) were produced against a highly immunogenic fraction derived by the chromatographic separation of the soluble preparation of axenic entamoeba histolytica (strain nih:200) trophozoites. isotype characterization of the six mabs revealed that four belonged to the igm class and one each to the igg1 and the igg2a subclasses. the immunoreactivity patterns and the specificity of the mabs with homologous and heterologous antigens were analyzed by the enzyme-linked immunot ...19938292992
human intestinal parasites in karakuak, west flores, indonesia and the effect of treatment with mebendazole and pyrantel pamoate.a survey for intestinal parasites and mass-treatment with a combination of mebendazole and pyrantel pamoate were conducted in karakuak, west flores in 1977. a total of 198 stool specimens from 104 males and 94 females ranging in age from less than 1 to 70 years were examined and 72% harbored one or more intestinal parasites. ascaris lumbricoides (43%) and entamoeba histolytica (21%) were the most common, followed by entamoeba coli (19%), hookworm (18%), iodamoeba bütschlii (8%), giardia lamblia ...19807444573
intestinal parasites of man in bukidnon, philippines, with emphasis on schistosomiasis.a total of 831 fecal specimens from malay-balay, mindanao, philippines were examined for schistosomiasis and common intestinal parasites. schistosoma japonicum eggs were found in 16% of the samples. infections were more common in males (20%) than in females (12%). age-wise, infections were infrequently diagnosed in children less than 10 years and infections in older age groups varied from 12-27%. hookworm, chiefly necator americanus, was the most common parasite found (44%) followed by ascaris l ...19817256354
intestinal parasites of man in northern bohol, philippines, with emphasis on schistosomiasis.a survey for intestinal parasites with emphasis on oriental schistosomiasis was conducted in the townships of trinidad and talibon, bohol province, philippines and approximately 1,700 stool samples were examined. schistosoma japonicum is still endemic to these areas of northern bohol but infection rates were much lower than expected, 5% rather than 30%. soil-transmitted helminths were the most common parasitic infections. hookworms were found in 71% of the samples tested, most infections were du ...19807221688
[contribution to the knowledge of intestinal protozoa infestation in the hospital population of barcelona (author's transl)].this report concerns the analysis of 650 fecal samples submitted for parasitic investigation to the microbiology service of the hospital de la santa creu i sant pau of barcelona. the samples were analyzed by the technique of fixation and direct observation of sapero and lawless (mifd), and with the biphasic concentration method of blagg et al (mifc). intestinal protozoa were detected in 183 samples (28.2%), with a total of 232 protozoosis uncovered. the specific analysis gave the following resul ...19817206888
safety and efficacy of diphetarsone in the treatment of amoebiasis, non-pathogenic amoebiasis and trichuriasis.eighty-nine patients infected with entamoeba histolytica, non-pathogenic amoebae or trichuris trichiura were studied prospectively to determine the safety and efficacy of diphetarsone therapy. an additional 75 patients were studied retrospectively to assess further the efficacy of diphetarsone in the treatment of e. histolytica cyst passers. side effects were noted in 9% and included gastrointestinal upset, lightheadedness and headache. transient liver function abnormalities were recorded in 5.6 ...19836304952
a zymodeme study of entamoeba histolytica in a group of south african schoolchildren.using a biphasic culture medium, stocks of intestinal amoebae were isolated from a group of children attending school in durban, south africa. these were compared with stocks collected in other areas of the world already characterized using the electrophoretic patterns of four enzymes: glucose phosphate isomerase (gpi), phosphoglucomutase (pgm) l-malate: nadp+ oxido-reductase (oxalacetate-decarboxylating) (me) and hexokinase (hk). 33% of 94 samples grew entamoeba histolytica, only one of which g ...19826287686
the epidemiology of entamoeba histolytica in a rural and an urban area of mexico. a pilot survey ii.stocks of intestinal amoebae grown in monoxenic culture, were compared against each other and against those previously reported from mexico city. these were isolated from subjects in san cristobal de las casas, chiapas (rural area) and hospital patients in merida, yucatan (urban area). electrophoretic patterns of the four enzymes: glucose phosphate isomerase (gpi), phosphoglucomutase (pgm), l-malate: nadp+ oxido-reductase (oxalacetate-decarboxylating) (me) and hexokinase (hk) demonstrated the pr ...19826285556
enteric pathogenic protozoa in homosexual men from san francisco.the prevalence of enteric protozoa was studied in a survey conducted among 150 male homosexual patients in san francisco. all patients were from a private practice in internal medicine. each was asked to complete a questionnaire and to submit multiple stool specimens for examination. of this group, 47% were positive for one or more potentially pathogenic intestinal protozoa; entamoeba histolytica was found in 36%, entamoeba hartmanni in 35%, giardia lamblia in 5%, and dientamoeba fragilis in 1.3 ...19846087479
human cysticercosis and intestinal parasitism amongst the ekari people of irian jaya.a random sample of 242 people showed that 42 had palpable cysts of taenia solium. faecal examination recovered eggs of t. solium in seven (3%), while trichuris (83%), ascaris (83%), hookworms (76%), strongyloides stercoralis (10%) and strongyloides sp. (29%), entamoeba histolytica (14%), entamoeba coli (22%), entamoeba hartmanni (7%), entamoeba polecki (7%), balantidium coli (9%) and dientamoeba fragilis (21%) were the most common other intestinal parasites encountered. elisa tests, using antige ...19873430662
gastrointestinal parasitoses discovered in agricultural workers in south bohemia, czechoslovakia.in the years from 1975 to 1982 1,750 persons, mostly employed by agricultural enterprises in the south bohemian region in czechoslovakia, were examined. we discovered seven species of parasites: taenia saginata in 0.3%, enterobius vermicularis in 10.1%, giardia lamblia in 1.0%, endolimax nana in 0.8%, entamoeba coli in 0.7%, entamoeba hartmanni in 0.2%, and chilomastix mesnili in 0.5%. the greatest number of parasites was found in students of the secondary agricultural and technical school. only ...19883169645
prevalence of enteric parasites in homosexual patients attending an outpatient clinic.a total of 372 pooled stool specimens from 274 homosexual men with diarrhea were submitted for parasitologic examination over a 2.5-year period. each two-vial pooled specimen set contained portions of stool from 3 consecutive days in formalin and polyvinyl alcohol. of the 274 patients, 133 (48.5%) harbored one or more intestinal protozoa, with 161 (43.3%) of the 372 specimens submitted being positive for one or more organisms. the parasites identified included entamoeba histolytica (71 patients) ...19862877006
coproantibody study in entamoeba histolytica, entamoeba hartmanni and other parasitic infections. 19852870983
use of a monoclonal antibody in an enzyme immunoassay for the detection of entamoeba histolytica in fecal specimens.an indirect enzyme-linked immunosorbent assay (elisa) was developed for the detection of entamoeba histolytica in human feces, using both a monoclonal antibody and rabbit antisera. it detected from less than 1 to 57 trophozoites of 6 e. histolytica strains. stool specimens were positive by elisa in 18 of 22 (82%) patients with e. histolytica and in 3 of 186 (2%) of patients without demonstrable e. histolytica in their stools. the latter included one from a child living near an asymptomatic cyst ...19852860814
entamoeba histolytica and other parasitic infections in south kanara district, karnataka.single stool specimens collected from 1,020 apparently healthy people of the south kanara district, were processed for intestinal parasites using three parasitological methods viz. (a) direct smear in physiological saline and d'antoni's iodine, (b) zinc sulfate concentration method and (c) by culture in modified boek and drbolhav medium. of these 781 (76.51 per cent) were found to be infected with parasites. the prevalence of various intestinal parasites was: ascaris lumbricoides (48.33 per cent ...19892559118
coincidence of deficient delayed hypersensitivity and intestinal protozoa in homosexual men.stool samples of 134 subjectively and apparently healthy male homosexual volunteers were studied, and in 86 men (64.2%) 190 parasite infestations, up to 6 per host, were identified. the most frequent species were endolimax nana (40), entamoeba coli (38), entamoeba histolytica (34), entamoeba hartmanni (32), and iodamoeba bütschlii (22); cryptosporidium was not found. all 11 isolates of entamoeba histolytica analysed belonged to a noninvasive zymodeme: 10 were of zymodeme i and i of zymodeme iii. ...19892555912
identification of a pathogenic isolate-specific 30,000-mr antigen of entamoeba histolytica by using a monoclonal antibody.a monoclonal antibody (mab) produced against trophozoites of entamoeba histolytica strain hm-1:imss, reacted with all of 42 isolates and 4 clones showing pathogenic zymodeme (z) patterns, i.e., z-ii, z-ii alpha-, z-ii (glucose phosphate isomerase: gamma +), z-vii, z-vii (glucose phosphate isomerase: alpha lack, gamma +), z-xi, z-xiv, and z-xix, regardless of culture conditions, geographical origins, or host symptoms in an indirect fluorescence antibody test. in contrast, the mab failed to react ...19902180826
entamoeba hartmanni: missing or misidentified? 19911869851
[blastocystis hominis in canavese: a retrospective study of samples received for fecal parasitological examination at the ivrea- castellamonte hospital over 42 months].in the last ten years the interest in the controversial blastocystis hominis microorganism of the intestine has greatly increased. the authors conducted a retrospective study of feces for parasitology investigations at the ivrea-castellamonte hospital. these investigations lasted 42 months (1/1/1988-31/6/1991) and the authors focused their attention on samples with 5 or more blastocystis hominis at the microscopically examination with a 40x phase objective. during the study 2,138 samples of fece ...19911844921
[entamoeba histolytica: a standing threat].the importance and limitations of microscopic differential diagnosis of entamoeba histolytica in asymptomatic carriers is reviewed. the possibility that some nonpathogenic strains of e. histolytica could be entamoeba hartmanni is discussed. the imperative necessity to encourage research in epidemiology and diagnosis is emphasized. the need to know the prevalence in mexico and distinguish e. histolytica from e. hartmanni is also discussed. this differential diagnosis is fundamental in the treatme ...19921615351
surveillance of some infectious diseases among aircrew personnel in southeast asia.a 2-year analysis of specimens among aircrew personnel in southeast asia is reported. stool specimens were examined for intestinal parasites and enteric bacteria, blood smears for blood parasites, and sera tested for transaminases (sgot) and antibodies to entamoeba histolytica, toxoplasma gondii, chikungunya and japanese encephalitis viruses, rickettsia tsutsugamushi, leptospira sp. and pseudomonas pseudomallei. one to four specimens each were obtained from 537 adult males and 20 females. there ...19751100040
intestinal and blood parasites of man in bireuen and takengon, aceh province, sumatra, indonesia.a survey for blood and intestinal parasites was carried out in aceh province of north sumatra, indonesia. a total of 348 stool specimens were obtained from 167 males and 181 females ranging in age from 6 months to 70 years. over 98% of the population sampled were found infected with at least one intestinal parasite. ascaris lumbricoides, trichuris trichiura, hookworm and entamoeba coli, in that order, were the most common parasites detected. other intestinal parasites found less frequently were ...19761030850
observations on the intestinal protozoa infecting man in rhodesia.humans in rhodesia harbour a wide range of intestinal protozoa. of the species included, entamoeba histolytica, entamoeba coli and giardia lamblia have previously been recorded. other species which are either rarely reported or which have previously never been reported from this country, include trichomonas hominis, chilomastix mesnili, enteromonas hominis, retortamonas intestinalis, balantidum coli,entamoeba hartmanni,entamoeba histolytica laredo. endolimax nana, dientamoeba fragilis and isospo ...1976988643
parasitology survey in northern sumatra, indonesia.a parasitology survey was conducted in five villages in north sumatra, indonesia. a total of 3,207 blood smears, 2,066 stool specimens and 969 sera were examined. sixty (1.9%) inhabitants had malaria (plasmodium vivax 41, p. falciparum 19), and 20 had brugia malayi microfilaraemia. the most common intestinal helminths were trichuris trichiura (87%), ascaris lumbricoides (75%) and hookworm (58%). other helminths found in low numbers were enterobius vermicularis, strongyloides stercoralis, taenia ...1976950682
parasitic infections in humans in west kalimantan (borneo), indonesia.a survey was carried out among inhabitants of eight villages in west kalimantan province (borneo), whereby blood smears were examined for malaria, stools examined for intestinal parasites and sera tested by the indirect hemagglutination test for antibodies to entamoeba histolytica and toxoplasma gondii. the prevalence of malaria among 3017 people examined was 5.6% (plasmodium vivax 2.8%, plasmodium falciparum 2.8%). brugia malayi microfilariae were found in 3.6% and wuchereria bancrofti in 0.3%. ...1976788263
electrophoretic isoenzyme patterns of the pathogenic and non-pathogenic intestinal amoebae of man.cultured stocks of entamoeba hartmanni, endolimax nana, iodamoeba buetschlli and dientamoeba fragilis were compared with the four entamoeba histolytical groups already described (sargeaunt et al., 1978), by the electrophoretic patterns of three enzymes: glucose phosphate isomerase (gpi), phosphoglucomutase (pgm) and l-malate: nadp+ oxidoreductase (oxalacetate-decarboxylating) (me). all the species were easily distinguished by their characteristic patterns.1979473310
human parasitoses of the malili area, south sulawesi (celebes) province, indonesia.a biomedical survey was conducted in 9 villages in the malili area of south sulawesi, indonesia. blood specimens were examined for malaria and microfilariae; stool specimens were examined for intestinal parasites. malaria parasitemias were rare; plasmodium falciparum was detected in 10 and p. vivax in 11 of 985 blood smears. malayan filariasis was endemic to all villages surveyed. the overall prevalence of detectable microfilaremias was 15%, varying from 34% in kawata to 1% in nuha. microfilaria ...1978364674
heat-labile enterotoxigenic escherichia coli and intestinal protozoa in asymptomatic travellers.thirty-two asymptomatic travellers who had recently journeyed in the near, middle, and far east and had experienced a high incidence of diarrhoeal disease were screened for heat-labile enterotoxigenic escherichia coli (ent+ e. coli) and other bacterial and parasitic pathogens. six percent were colonized with ent+ e. coli and while other bacterial pathogens were not found, the intestinal protozoa giardia lamblia (13%), entamoeba histolytica (6%), entamoeba coli (6%), endolimax nana (6%), and enta ...1977351820
biomedical survey in irian jaya (west irian), indonesia.a biomedical survey was conducted in several areas of irian jaya, indonesia in july 1972 in association with an investigation of reports of a cholera outbreak. stool specimens, blood smears and sera were collected and examined for evidence of parasitic as well as other infectious diseases. a total of 114 stools were examined and the most commonly found intestinal parasites were trichuris trichiura (94%), ascaris lumbricoides (74%), hookworm (58%), entamoeba coli (15%), endolimax nana (8%), entam ...1977208184
double-blind test of metronidazole and tinidazole in the treatment of asymptomatic entamoeba histolytica and entamoeba hartmanni carriers.one hundred and fifteen persons with asymptomatic entamoeba histolytica or e. hartmanni infection, or both, were given metronidazole (750 mg three times daily for 5 days), tinidazole (1 g twice daily on 2 consecutive days), or a starch placebo. three post-treatment stools were examined in the 2 weeks following initiation of treatment. cysts of e. histolytica reappeared in the stools of 37% of 30 given metronidazole, 62% of 34 given tinidazole, and 70% of 31 given placebo. cysts of e. hartmanni r ...1976183554
parasitology survey and seroepidemiology of amoebiasis in south kalimantan (borneo), indonesia.a parasitology surevy was conducted among inhabitants of 7 villages in 3 regencies in south kalimantan province, indonesia. a total of 2,169 stool specimens, 2,756 blood smears and 1,027 serum specimens were obtained, representing samples from approximately 10%, 12% and 5% of the population, respectively. one to 8 different intestinal parasitic infections were detected in 97% of the people. those parasites most frequently found were trichuris trichiura (83%), ascaris lumbricoides (79%), and hook ...1975167454
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